AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99509


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99509.t1 MTR_7g070070 99.200 250 2 0 1 250 1 250 0.0 513
MS.gene99509.t1 MTR_5g016590 98.000 250 5 0 1 250 1 250 0.0 508
MS.gene99509.t1 MTR_1g112250 45.161 217 107 5 5 215 8 218 4.29e-58 184
MS.gene99509.t1 MTR_8g075320 42.500 200 112 3 4 202 3 200 2.44e-53 173
MS.gene99509.t1 MTR_2g016050 41.500 200 114 3 4 202 3 200 4.91e-51 167
MS.gene99509.t1 MTR_0167s0060 38.571 210 126 3 4 213 5 211 1.43e-47 157
MS.gene99509.t1 MTR_8g464460 38.136 236 138 4 5 237 9 239 3.36e-44 149
MS.gene99509.t1 MTR_1g022410 38.785 214 125 3 4 215 5 214 2.48e-43 148
MS.gene99509.t1 MTR_2g060830 37.815 238 136 6 5 237 9 239 2.87e-42 144
MS.gene99509.t1 MTR_6g024135 35.071 211 135 2 5 215 8 216 1.58e-37 132
MS.gene99509.t1 MTR_4g012740 28.968 252 135 6 4 215 236 483 5.74e-22 94.7
MS.gene99509.t1 MTR_6g013300 32.632 190 111 7 33 215 138 317 1.78e-16 77.8
MS.gene99509.t1 MTR_4g057090 29.947 187 119 5 26 207 110 289 2.00e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99509.t1 AT3G22110 90.000 250 25 0 1 250 1 250 1.51e-172 475
MS.gene99509.t1 AT4G15165 72.000 250 28 1 1 250 1 208 7.59e-127 358
MS.gene99509.t1 AT3G14290 45.161 217 107 5 5 215 8 218 2.76e-58 185
MS.gene99509.t1 AT1G53850 44.240 217 109 5 5 215 8 218 2.49e-57 182
MS.gene99509.t1 AT1G53850 44.240 217 109 5 5 215 8 218 2.49e-57 182
MS.gene99509.t1 AT5G66140 42.000 200 113 3 4 202 3 200 8.81e-53 171
MS.gene99509.t1 AT3G51260 42.500 200 112 3 4 202 3 200 1.12e-52 171
MS.gene99509.t1 AT3G51260 42.500 200 112 3 4 202 3 200 1.13e-52 171
MS.gene99509.t1 AT1G16470 37.264 212 130 3 4 215 5 213 9.89e-47 155
MS.gene99509.t1 AT1G16470 37.264 212 130 3 4 215 5 213 9.89e-47 155
MS.gene99509.t1 AT1G79210 37.264 212 130 3 4 215 5 213 1.18e-46 155
MS.gene99509.t1 AT1G79210 37.264 212 130 3 4 215 5 213 1.18e-46 155
MS.gene99509.t1 AT1G79210 37.264 212 130 3 4 215 5 213 1.18e-46 155
MS.gene99509.t1 AT5G42790 39.320 206 119 3 4 207 5 206 3.72e-44 150
MS.gene99509.t1 AT1G47250 38.350 206 121 3 4 207 5 206 2.55e-43 148
MS.gene99509.t1 AT5G35590 40.704 199 114 3 5 200 9 206 3.68e-42 144
MS.gene99509.t1 AT2G05840 38.191 199 119 3 5 200 9 206 8.34e-39 135
MS.gene99509.t1 AT2G05840 38.191 199 119 3 5 200 9 206 1.04e-38 135
MS.gene99509.t1 AT2G05840 38.235 204 115 4 5 205 9 204 5.05e-38 132
MS.gene99509.t1 AT2G27020 33.493 209 137 2 5 213 8 214 2.86e-36 129
MS.gene99509.t1 AT2G27020 33.175 211 139 2 5 215 110 318 3.52e-35 129

Find 62 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTCTCTTTCCTATTTGC+AGG 0.250981 7.1:+32151162 MS.gene99509:CDS
GTCAAAAGACGGGGTTGTTC+TGG 0.306994 7.1:+32150885 MS.gene99509:CDS
ACTAAGCTATTGGTGAAGTC+TGG 0.325538 7.1:+32151471 MS.gene99509:CDS
CTCTTTCCTATTTGCAGGCT+GGG 0.331080 7.1:+32151167 MS.gene99509:CDS
AGCAGTTCAACTTGCATTGA+AGG 0.356948 7.1:+32151332 MS.gene99509:CDS
GGATCCTAGCAGTATTGATT+AGG 0.366704 7.1:-32151026 None:intergenic
AGCAGTGTCTGTTGCTGGTT+GGG 0.371924 7.1:-32151499 None:intergenic
TTCGGTGGTCTCCGACCATT+TGG 0.375556 7.1:+32151141 MS.gene99509:CDS
AGAGAATTTGACTAAGCTAT+TGG 0.375935 7.1:+32151461 MS.gene99509:CDS
GATTGAACCAACTGCTCAAC+AGG 0.380070 7.1:-32151087 None:intergenic
TCTCTTTCCTATTTGCAGGC+TGG 0.384561 7.1:+32151166 MS.gene99509:CDS
GAAGGTTCTGAGTAAAACTA+TGG 0.403623 7.1:+32151350 MS.gene99509:CDS
GGTTGGAAAGCTGGTGCTAT+TGG 0.408203 7.1:+32151243 MS.gene99509:CDS
AGCTAAGCAGTGTCTGTTGC+TGG 0.410039 7.1:-32151504 None:intergenic
GGAAATGCTGGTACTGCAAT+AGG 0.415260 7.1:+32150856 MS.gene99509:CDS
TGACTGTGCTGCCTGGTTGT+TGG 0.416766 7.1:-32151268 None:intergenic
CATGTAGCATGTGCTGTCGC+TGG 0.433619 7.1:+32150982 MS.gene99509:CDS
AGTGATCCAAGCGGAAACTA+TGG 0.434323 7.1:+32151219 MS.gene99509:CDS
AGCACAGTCAATTCTAAAAC+AGG 0.450248 7.1:+32151281 MS.gene99509:CDS
GCTCAACAGGCATTGGCTCT+TGG 0.472402 7.1:-32151074 None:intergenic
TTAGAATTGACTGTGCTGCC+TGG 0.476900 7.1:-32151275 None:intergenic
AACTATGGTGGTTGGAAAGC+TGG 0.479761 7.1:+32151234 MS.gene99509:CDS
GCCAATGCCTGTTGAGCAGT+TGG 0.481970 7.1:+32151080 MS.gene99509:CDS
ACTTGGTACAGACGTCCTTC+AGG 0.486610 7.1:-32150811 None:intergenic
AAGCAAGGATACACACAATT+CGG 0.488228 7.1:+32151123 MS.gene99509:CDS
AAGCAGTGTCTGTTGCTGGT+TGG 0.492638 7.1:-32151500 None:intergenic
CTAAGCTATTGGTGAAGTCT+GGG 0.494770 7.1:+32151472 MS.gene99509:CDS
AGCAGTATTGATTAGGATGT+TGG 0.498265 7.1:-32151019 None:intergenic
TAGGAATCTTGTCAAAAGAC+GGG 0.510970 7.1:+32150875 MS.gene99509:CDS
CAAAGGAGTAACGTTGTGCT+TGG 0.511294 7.1:-32151047 None:intergenic
ATGGAGGCCATCGGAAATGC+TGG 0.514078 7.1:+32150844 MS.gene99509:CDS
CAACCACCATAGTTTCCGCT+TGG 0.517588 7.1:-32151225 None:intergenic
ACCAACTGCTCAACAGGCAT+TGG 0.521861 7.1:-32151081 None:intergenic
CAGGAGAGAATATTGTTGTA+CGG 0.539096 7.1:-32150792 None:intergenic
TGCTATTGGTGCCAACAACC+AGG 0.541336 7.1:+32151257 MS.gene99509:CDS
TGTTCTGGTTGGTGAAAAGA+AGG 0.542861 7.1:+32150900 MS.gene99509:CDS
CAAGCGGAAACTATGGTGGT+TGG 0.545365 7.1:+32151226 MS.gene99509:CDS
AGTTGAGGTTTGAAGCAGCT+TGG 0.546459 7.1:-32150929 None:intergenic
ATGTAGCATGTGCTGTCGCT+GGG 0.546740 7.1:+32150983 MS.gene99509:CDS
AATTCTAAAACAGGACTACA+AGG 0.553889 7.1:+32151290 MS.gene99509:CDS
CCTCCATTGCATACTCCACT+TGG 0.562163 7.1:-32150828 None:intergenic
ATAGGAATCTTGTCAAAAGA+CGG 0.567737 7.1:+32150874 MS.gene99509:CDS
AAAGACGGGGTTGTTCTGGT+TGG 0.578404 7.1:+32150889 MS.gene99509:CDS
ACATCCTAATCAATACTGCT+AGG 0.578961 7.1:+32151022 MS.gene99509:CDS
AATAGGAAAGAGACTCCAAA+TGG 0.587032 7.1:-32151156 None:intergenic
CCAAGTGGAGTATGCAATGG+AGG 0.592642 7.1:+32150828 MS.gene99509:CDS
CATTGGCTCTTGGTAAGCAA+AGG 0.596164 7.1:-32151064 None:intergenic
TGCAGTACCAGCATTTCCGA+TGG 0.599600 7.1:-32150851 None:intergenic
GTACCAAGTGGAGTATGCAA+TGG 0.605368 7.1:+32150825 MS.gene99509:CDS
ACAACAATATTCTCTCCTGA+AGG 0.618706 7.1:+32150796 MS.gene99509:CDS
GGAAAGAGACTCCAAATGGT+CGG 0.621181 7.1:-32151152 None:intergenic
GAGTATGCAATGGAGGCCAT+CGG 0.626386 7.1:+32150835 MS.gene99509:CDS
TCTCTCTGTGATACTAAGCA+AGG 0.640603 7.1:+32151108 MS.gene99509:CDS
GATCCAAGCGGAAACTATGG+TGG 0.659270 7.1:+32151222 MS.gene99509:CDS
CAAGGATACACACAATTCGG+TGG 0.675083 7.1:+32151126 MS.gene99509:CDS
TGAAGGACGTCTGTACCAAG+TGG 0.675276 7.1:+32150813 MS.gene99509:CDS
AGGAATCTTGTCAAAAGACG+GGG 0.680553 7.1:+32150876 MS.gene99509:CDS
ACTACAAGGATGATATCACA+AGG 0.683851 7.1:+32151304 MS.gene99509:CDS
TAGCTTAGTCAAATTCTCTG+AGG 0.695782 7.1:-32151457 None:intergenic
TAAGCTATTGGTGAAGTCTG+GGG 0.701098 7.1:+32151473 MS.gene99509:CDS
CTTTACATGAGTGATCCAAG+CGG 0.758885 7.1:+32151210 MS.gene99509:CDS
CTACAAGGATGATATCACAA+GGG 0.766354 7.1:+32151305 MS.gene99509:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAGAATTTGACTAAGCTAT+TGG + chr7.1:32151461-32151480 MS.gene99509:CDS 30.0%
ATAGGAATCTTGTCAAAAGA+CGG + chr7.1:32150874-32150893 MS.gene99509:CDS 30.0%
!! AATTCTAAAACAGGACTACA+AGG + chr7.1:32151290-32151309 MS.gene99509:CDS 30.0%
AAGCAAGGATACACACAATT+CGG + chr7.1:32151123-32151142 MS.gene99509:CDS 35.0%
AATAGGAAAGAGACTCCAAA+TGG - chr7.1:32151159-32151178 None:intergenic 35.0%
ACAACAATATTCTCTCCTGA+AGG + chr7.1:32150796-32150815 MS.gene99509:CDS 35.0%
ACATCCTAATCAATACTGCT+AGG + chr7.1:32151022-32151041 MS.gene99509:CDS 35.0%
ACTACAAGGATGATATCACA+AGG + chr7.1:32151304-32151323 MS.gene99509:CDS 35.0%
CAGGAGAGAATATTGTTGTA+CGG - chr7.1:32150795-32150814 None:intergenic 35.0%
CTACAAGGATGATATCACAA+GGG + chr7.1:32151305-32151324 MS.gene99509:CDS 35.0%
TAGCTTAGTCAAATTCTCTG+AGG - chr7.1:32151460-32151479 None:intergenic 35.0%
TAGGAATCTTGTCAAAAGAC+GGG + chr7.1:32150875-32150894 MS.gene99509:CDS 35.0%
! CATTTTCTCAGTTGAAGTTG+AGG - chr7.1:32150947-32150966 None:intergenic 35.0%
! TGATACTTGACTTTTCCAGA+GGG - chr7.1:32151429-32151448 None:intergenic 35.0%
! TTGATACTTGACTTTTCCAG+AGG - chr7.1:32151430-32151449 None:intergenic 35.0%
!! AGCACAGTCAATTCTAAAAC+AGG + chr7.1:32151281-32151300 MS.gene99509:CDS 35.0%
!! AGCAGTATTGATTAGGATGT+TGG - chr7.1:32151022-32151041 None:intergenic 35.0%
!! GAAGGTTCTGAGTAAAACTA+TGG + chr7.1:32151350-32151369 MS.gene99509:CDS 35.0%
ACTAAGCTATTGGTGAAGTC+TGG + chr7.1:32151471-32151490 MS.gene99509:CDS 40.0%
AGCAGTTCAACTTGCATTGA+AGG + chr7.1:32151332-32151351 MS.gene99509:CDS 40.0%
AGGAATCTTGTCAAAAGACG+GGG + chr7.1:32150876-32150895 MS.gene99509:CDS 40.0%
CTAAGCTATTGGTGAAGTCT+GGG + chr7.1:32151472-32151491 MS.gene99509:CDS 40.0%
CTTTACATGAGTGATCCAAG+CGG + chr7.1:32151210-32151229 MS.gene99509:CDS 40.0%
TAAGCTATTGGTGAAGTCTG+GGG + chr7.1:32151473-32151492 MS.gene99509:CDS 40.0%
TCTCTCTGTGATACTAAGCA+AGG + chr7.1:32151108-32151127 MS.gene99509:CDS 40.0%
! GAAGTTTTTCTTTCACCCTC+TGG + chr7.1:32151411-32151430 MS.gene99509:CDS 40.0%
! TGCAAGTTCAAGCTTTTCTG+AGG - chr7.1:32151391-32151410 None:intergenic 40.0%
!! GGATCCTAGCAGTATTGATT+AGG - chr7.1:32151029-32151048 None:intergenic 40.0%
!! TGTTCTGGTTGGTGAAAAGA+AGG + chr7.1:32150900-32150919 MS.gene99509:CDS 40.0%
AACTATGGTGGTTGGAAAGC+TGG + chr7.1:32151234-32151253 MS.gene99509:CDS 45.0%
AGTGATCCAAGCGGAAACTA+TGG + chr7.1:32151219-32151238 MS.gene99509:CDS 45.0%
CAAAGGAGTAACGTTGTGCT+TGG - chr7.1:32151050-32151069 None:intergenic 45.0%
CAAGGATACACACAATTCGG+TGG + chr7.1:32151126-32151145 MS.gene99509:CDS 45.0%
CTCTTTCCTATTTGCAGGCT+GGG + chr7.1:32151167-32151186 MS.gene99509:CDS 45.0%
GATTGAACCAACTGCTCAAC+AGG - chr7.1:32151090-32151109 None:intergenic 45.0%
GCAGGCTGGGACAAAAATTT+CGG + chr7.1:32151180-32151199 MS.gene99509:CDS 45.0%
GGAAAGAGACTCCAAATGGT+CGG - chr7.1:32151155-32151174 None:intergenic 45.0%
GGAAATGCTGGTACTGCAAT+AGG + chr7.1:32150856-32150875 MS.gene99509:CDS 45.0%
GGAGTCTCTTTCCTATTTGC+AGG + chr7.1:32151162-32151181 MS.gene99509:CDS 45.0%
GTACCAAGTGGAGTATGCAA+TGG + chr7.1:32150825-32150844 MS.gene99509:CDS 45.0%
TCTCTTTCCTATTTGCAGGC+TGG + chr7.1:32151166-32151185 MS.gene99509:CDS 45.0%
TTAGAATTGACTGTGCTGCC+TGG - chr7.1:32151278-32151297 None:intergenic 45.0%
! AGTTGAGGTTTGAAGCAGCT+TGG - chr7.1:32150932-32150951 None:intergenic 45.0%
! TTTTGTCCCAGCCTGCAAAT+AGG - chr7.1:32151176-32151195 None:intergenic 45.0%
!! CATTGGCTCTTGGTAAGCAA+AGG - chr7.1:32151067-32151086 None:intergenic 45.0%
AAAGACGGGGTTGTTCTGGT+TGG + chr7.1:32150889-32150908 MS.gene99509:CDS 50.0%
AAGCAGTGTCTGTTGCTGGT+TGG - chr7.1:32151503-32151522 None:intergenic 50.0%
ACCAACTGCTCAACAGGCAT+TGG - chr7.1:32151084-32151103 None:intergenic 50.0%
ACTTGGTACAGACGTCCTTC+AGG - chr7.1:32150814-32150833 None:intergenic 50.0%
ATGTAGCATGTGCTGTCGCT+GGG + chr7.1:32150983-32151002 MS.gene99509:CDS 50.0%
CAACCACCATAGTTTCCGCT+TGG - chr7.1:32151228-32151247 None:intergenic 50.0%
CAAGCGGAAACTATGGTGGT+TGG + chr7.1:32151226-32151245 MS.gene99509:CDS 50.0%
CCAAGTGGAGTATGCAATGG+AGG + chr7.1:32150828-32150847 MS.gene99509:CDS 50.0%
CCTCCATTGCATACTCCACT+TGG - chr7.1:32150831-32150850 None:intergenic 50.0%
GAGTATGCAATGGAGGCCAT+CGG + chr7.1:32150835-32150854 MS.gene99509:CDS 50.0%
GATCCAAGCGGAAACTATGG+TGG + chr7.1:32151222-32151241 MS.gene99509:CDS 50.0%
GTCAAAAGACGGGGTTGTTC+TGG + chr7.1:32150885-32150904 MS.gene99509:CDS 50.0%
TGAAGGACGTCTGTACCAAG+TGG + chr7.1:32150813-32150832 MS.gene99509:CDS 50.0%
TGCAGTACCAGCATTTCCGA+TGG - chr7.1:32150854-32150873 None:intergenic 50.0%
! AGCAGTGTCTGTTGCTGGTT+GGG - chr7.1:32151502-32151521 None:intergenic 50.0%
!! GGTTGGAAAGCTGGTGCTAT+TGG + chr7.1:32151243-32151262 MS.gene99509:CDS 50.0%
!! TGCTATTGGTGCCAACAACC+AGG + chr7.1:32151257-32151276 MS.gene99509:CDS 50.0%
ATGGAGGCCATCGGAAATGC+TGG + chr7.1:32150844-32150863 MS.gene99509:CDS 55.0%
CATGTAGCATGTGCTGTCGC+TGG + chr7.1:32150982-32151001 MS.gene99509:CDS 55.0%
GCCAATGCCTGTTGAGCAGT+TGG + chr7.1:32151080-32151099 MS.gene99509:CDS 55.0%
TGACTGTGCTGCCTGGTTGT+TGG - chr7.1:32151271-32151290 None:intergenic 55.0%
TTCGGTGGTCTCCGACCATT+TGG + chr7.1:32151141-32151160 MS.gene99509:CDS 55.0%
!! GCTCAACAGGCATTGGCTCT+TGG - chr7.1:32151077-32151096 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 32150772 32151524 32150772 ID=MS.gene99509
chr7.1 mRNA 32150772 32151524 32150772 ID=MS.gene99509.t1;Parent=MS.gene99509
chr7.1 exon 32150772 32151524 32150772 ID=MS.gene99509.t1.exon1;Parent=MS.gene99509.t1
chr7.1 CDS 32150772 32151524 32150772 ID=cds.MS.gene99509.t1;Parent=MS.gene99509.t1
Gene Sequence

>MS.gene99509

ATGTCTCGAAGATATGATAGCCGTACAACAATATTCTCTCCTGAAGGACGTCTGTACCAAGTGGAGTATGCAATGGAGGCCATCGGAAATGCTGGTACTGCAATAGGAATCTTGTCAAAAGACGGGGTTGTTCTGGTTGGTGAAAAGAAGGTGACATCCAAGCTGCTTCAAACCTCAACTTCAACTGAGAAAATGTACAAGATCGATGATCATGTAGCATGTGCTGTCGCTGGGATTATGTCTGACGCCAACATCCTAATCAATACTGCTAGGATCCAAGCACAACGTTACTCCTTTGCTTACCAAGAGCCAATGCCTGTTGAGCAGTTGGTTCAATCTCTCTGTGATACTAAGCAAGGATACACACAATTCGGTGGTCTCCGACCATTTGGAGTCTCTTTCCTATTTGCAGGCTGGGACAAAAATTTCGGCTTTCAACTTTACATGAGTGATCCAAGCGGAAACTATGGTGGTTGGAAAGCTGGTGCTATTGGTGCCAACAACCAGGCAGCACAGTCAATTCTAAAACAGGACTACAAGGATGATATCACAAGGGAAGAAGCAGTTCAACTTGCATTGAAGGTTCTGAGTAAAACTATGGACAGCACTAGTCTTACCTCAGAAAAGCTTGAACTTGCAGAAGTTTTTCTTTCACCCTCTGGAAAAGTCAAGTATCAAGTTTGCTCCTCAGAGAATTTGACTAAGCTATTGGTGAAGTCTGGGGTGACCCAACCAGCAACAGACACTGCTTAG

Protein sequence

>MS.gene99509.t1

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGNAGTAIGILSKDGVVLVGEKKVTSKLLQTSTSTEKMYKIDDHVACAVAGIMSDANILINTARIQAQRYSFAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNFGFQLYMSDPSGNYGGWKAGAIGANNQAAQSILKQDYKDDITREEAVQLALKVLSKTMDSTSLTSEKLELAEVFLSPSGKVKYQVCSSENLTKLLVKSGVTQPATDTA