Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30368.t1 | XP_024629113.1 | 98.2 | 114 | 2 | 0 | 1 | 114 | 1 | 114 | 5.20E-50 | 206.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30368.t1 | Q570U6 | 70.8 | 113 | 33 | 0 | 1 | 113 | 1 | 113 | 2.1e-36 | 152.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30368.t1 | C6ZKI2 | 98.2 | 114 | 2 | 0 | 1 | 114 | 1 | 114 | 3.7e-50 | 206.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene30368 | 0.841136 | 5.71E-58 | -1.69E-46 |
| MS.gene049272 | MS.gene30368 | 0.80764 | 4.58E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene30368 | 0.814223 | 1.72E-51 | -1.69E-46 |
| MS.gene049669 | MS.gene30368 | 0.813378 | 2.64E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene30368 | 0.800871 | 1.18E-48 | -1.69E-46 |
| MS.gene050105 | MS.gene30368 | 0.805251 | 1.46E-49 | -1.69E-46 |
| MS.gene050633 | MS.gene30368 | 0.80723 | 5.59E-50 | -1.69E-46 |
| MS.gene051122 | MS.gene30368 | 0.810079 | 1.38E-50 | -1.69E-46 |
| MS.gene051332 | MS.gene30368 | 0.826375 | 2.81E-54 | -1.69E-46 |
| MS.gene051368 | MS.gene30368 | 0.828665 | 7.93E-55 | -1.69E-46 |
| MS.gene051541 | MS.gene30368 | 0.814736 | 1.32E-51 | -1.69E-46 |
| MS.gene051924 | MS.gene30368 | -0.824951 | 6.11E-54 | -1.69E-46 |
| MS.gene051926 | MS.gene30368 | -0.830137 | 3.48E-55 | -1.69E-46 |
| MS.gene052190 | MS.gene30368 | 0.804352 | 2.25E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene30368 | 0.853873 | 1.79E-61 | -1.69E-46 |
| MS.gene052464 | MS.gene30368 | 0.814372 | 1.59E-51 | -1.69E-46 |
| MS.gene052493 | MS.gene30368 | 0.839919 | 1.19E-57 | -1.69E-46 |
| MS.gene052534 | MS.gene30368 | 0.801467 | 8.88E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene30368 | 0.871669 | 5.73E-67 | -1.69E-46 |
| MS.gene052674 | MS.gene30368 | 0.839417 | 1.60E-57 | -1.69E-46 |
| MS.gene052675 | MS.gene30368 | 0.834865 | 2.34E-56 | -1.69E-46 |
| MS.gene052677 | MS.gene30368 | 0.839234 | 1.79E-57 | -1.69E-46 |
| MS.gene052685 | MS.gene30368 | 0.801087 | 1.06E-48 | -1.69E-46 |
| MS.gene052954 | MS.gene30368 | 0.805044 | 1.61E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene30368 | 0.817869 | 2.64E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene30368 | -0.841279 | 5.23E-58 | -1.69E-46 |
| MS.gene054467 | MS.gene30368 | 0.812044 | 5.17E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene30368 | 0.868873 | 4.72E-66 | -1.69E-46 |
| MS.gene054479 | MS.gene30368 | 0.86196 | 7.11E-64 | -1.69E-46 |
| MS.gene054576 | MS.gene30368 | 0.816095 | 6.60E-52 | -1.69E-46 |
| MS.gene054641 | MS.gene30368 | 0.808887 | 2.48E-50 | -1.69E-46 |
| MS.gene055327 | MS.gene30368 | 0.814762 | 1.31E-51 | -1.69E-46 |
| MS.gene055586 | MS.gene30368 | 0.825444 | 4.67E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene30368 | 0.842927 | 1.92E-58 | -1.69E-46 |
| MS.gene055864 | MS.gene30368 | 0.82315 | 1.62E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene30368 | 0.805806 | 1.12E-49 | -1.69E-46 |
| MS.gene056480 | MS.gene30368 | 0.815019 | 1.15E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene30368 | 0.830264 | 3.24E-55 | -1.69E-46 |
| MS.gene056779 | MS.gene30368 | 0.815705 | 8.06E-52 | -1.69E-46 |
| MS.gene05690 | MS.gene30368 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene30368 | 0.854437 | 1.23E-61 | -1.69E-46 |
| MS.gene057156 | MS.gene30368 | -0.830249 | 3.27E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene30368 | 0.806724 | 7.16E-50 | -1.69E-46 |
| MS.gene057598 | MS.gene30368 | 0.806779 | 6.97E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene30368 | 0.809942 | 1.47E-50 | -1.69E-46 |
| MS.gene058223 | MS.gene30368 | -0.817714 | 2.86E-52 | -1.69E-46 |
| MS.gene058289 | MS.gene30368 | 0.807695 | 4.46E-50 | -1.69E-46 |
| MS.gene058427 | MS.gene30368 | 0.812277 | 4.60E-51 | -1.69E-46 |
| MS.gene058429 | MS.gene30368 | 0.82515 | 5.48E-54 | -1.69E-46 |
| MS.gene058502 | MS.gene30368 | 0.809735 | 1.63E-50 | -1.69E-46 |
| MS.gene058897 | MS.gene30368 | 0.808257 | 3.38E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene30368 | 0.81951 | 1.12E-52 | -1.69E-46 |
| MS.gene059440 | MS.gene30368 | 0.812258 | 4.64E-51 | -1.69E-46 |
| MS.gene059952 | MS.gene30368 | 0.830446 | 2.92E-55 | -1.69E-46 |
| MS.gene060011 | MS.gene30368 | 0.808485 | 3.03E-50 | -1.69E-46 |
| MS.gene060012 | MS.gene30368 | 0.81635 | 5.79E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene30368 | 0.802756 | 4.82E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene30368 | 0.832945 | 7.08E-56 | -1.69E-46 |
| MS.gene06052 | MS.gene30368 | 0.851737 | 7.30E-61 | -1.69E-46 |
| MS.gene060850 | MS.gene30368 | 0.809137 | 2.20E-50 | -1.69E-46 |
| MS.gene060851 | MS.gene30368 | 0.817968 | 2.50E-52 | -1.69E-46 |
| MS.gene060852 | MS.gene30368 | 0.816039 | 6.79E-52 | -1.69E-46 |
| MS.gene060879 | MS.gene30368 | 0.867518 | 1.29E-65 | -1.69E-46 |
| MS.gene060932 | MS.gene30368 | 0.805824 | 1.11E-49 | -1.69E-46 |
| MS.gene06121 | MS.gene30368 | 0.800119 | 1.67E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30368.t1 | MTR_8g020200 | 98.246 | 114 | 2 | 0 | 1 | 114 | 1 | 114 | 5.25e-76 | 220 |
| MS.gene30368.t1 | MTR_2g041310 | 71.053 | 114 | 33 | 0 | 1 | 114 | 1 | 114 | 6.06e-55 | 167 |
| MS.gene30368.t1 | MTR_4g125590 | 70.796 | 113 | 32 | 1 | 1 | 112 | 1 | 113 | 9.38e-54 | 164 |
| MS.gene30368.t1 | MTR_3g070490 | 46.250 | 80 | 43 | 0 | 6 | 85 | 36 | 115 | 1.41e-21 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30368.t1 | AT1G17455 | 70.796 | 113 | 33 | 0 | 1 | 113 | 1 | 113 | 4.47e-53 | 162 |
| MS.gene30368.t1 | AT1G17455 | 70.796 | 113 | 33 | 0 | 1 | 113 | 1 | 113 | 4.47e-53 | 162 |
| MS.gene30368.t1 | AT1G72630 | 66.667 | 114 | 37 | 1 | 1 | 113 | 5 | 118 | 5.50e-49 | 152 |
| MS.gene30368.t1 | AT2G06255 | 60.870 | 115 | 38 | 3 | 1 | 114 | 1 | 109 | 4.65e-42 | 134 |
| MS.gene30368.t1 | AT2G29950 | 52.113 | 71 | 34 | 0 | 16 | 86 | 34 | 104 | 1.36e-18 | 75.5 |
| MS.gene30368.t1 | AT2G40080 | 47.143 | 70 | 37 | 0 | 16 | 85 | 22 | 91 | 1.39e-18 | 75.1 |
Find 16 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCCTAGACTCATGATTT+TGG | 0.224769 | 8.1:-78933246 | None:intergenic |
| GGTGATATATTTGGAGAATT+AGG | 0.240535 | 8.1:+78933118 | MS.gene30368:CDS |
| AACTTGATCTAATTCTCTTC+TGG | 0.282924 | 8.1:-78933432 | None:intergenic |
| GAAATGGAAGGTGATATATT+TGG | 0.290473 | 8.1:+78933109 | None:intergenic |
| ACATTTCTACTCAGATTATC+AGG | 0.344511 | 8.1:-78933268 | None:intergenic |
| AGACGAAGCTTCCCGCGACT+TGG | 0.462270 | 8.1:-78933365 | None:intergenic |
| AGTGGAACTCTCAAATCTGA+TGG | 0.488717 | 8.1:+78933400 | MS.gene30368:CDS |
| TAAACCAAAATCATGAGTCT+AGG | 0.526715 | 8.1:+78933242 | MS.gene30368:CDS |
| TTATCTGGTTGATTAAAAGC+CGG | 0.545205 | 8.1:-78933222 | None:intergenic |
| TCGCGGGAAGCTTCGTCTGA+AGG | 0.551915 | 8.1:+78933370 | MS.gene30368:CDS |
| GATAATCTGAGTAGAAATGT+TGG | 0.559396 | 8.1:+78933271 | MS.gene30368:CDS |
| AATTGTAATTGAGAAATGGA+AGG | 0.598993 | 8.1:+78933097 | None:intergenic |
| GAGTTCGTTTACCAAGTCGC+GGG | 0.624410 | 8.1:+78933354 | MS.gene30368:CDS |
| CGAGTTCGTTTACCAAGTCG+CGG | 0.624955 | 8.1:+78933353 | MS.gene30368:CDS |
| TCGTCTGAAGGAGATTCAAG+TGG | 0.640398 | 8.1:+78933382 | MS.gene30368:CDS |
| GAATAGTAATATCAGAAGAG+TGG | 0.647250 | 8.1:+78933309 | MS.gene30368:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATATTTTGAATCAAAAC+CGG | + | chr8.1:78933203-78933222 | MS.gene30368:CDS | 20.0% |
| AACTTGATCTAATTCTCTTC+TGG | - | chr8.1:78933435-78933454 | None:intergenic | 30.0% | |
| ACATTTCTACTCAGATTATC+AGG | - | chr8.1:78933271-78933290 | None:intergenic | 30.0% | |
| GAATAGTAATATCAGAAGAG+TGG | + | chr8.1:78933309-78933328 | MS.gene30368:CDS | 30.0% | |
| GATAATCTGAGTAGAAATGT+TGG | + | chr8.1:78933271-78933290 | MS.gene30368:CDS | 30.0% | |
| GGTGATATATTTGGAGAATT+AGG | + | chr8.1:78933118-78933137 | MS.gene30368:CDS | 30.0% | |
| TAAACCAAAATCATGAGTCT+AGG | + | chr8.1:78933242-78933261 | MS.gene30368:CDS | 30.0% | |
| TTATCTGGTTGATTAAAAGC+CGG | - | chr8.1:78933225-78933244 | None:intergenic | 30.0% | |
| ! | AGTAGATAGTAGAGTTTTAC+AGG | + | chr8.1:78933153-78933172 | MS.gene30368:CDS | 30.0% |
| !!! | ACTCATGATTTTGGTTTATC+TGG | - | chr8.1:78933240-78933259 | None:intergenic | 30.0% |
| ! | GAATCCTAGACTCATGATTT+TGG | - | chr8.1:78933249-78933268 | None:intergenic | 35.0% |
| AGTGGAACTCTCAAATCTGA+TGG | + | chr8.1:78933400-78933419 | MS.gene30368:CDS | 40.0% | |
| TCGTCTGAAGGAGATTCAAG+TGG | + | chr8.1:78933382-78933401 | MS.gene30368:CDS | 45.0% | |
| CGAGTTCGTTTACCAAGTCG+CGG | + | chr8.1:78933353-78933372 | MS.gene30368:CDS | 50.0% | |
| GAGTTCGTTTACCAAGTCGC+GGG | + | chr8.1:78933354-78933373 | MS.gene30368:CDS | 50.0% | |
| AGACGAAGCTTCCCGCGACT+TGG | - | chr8.1:78933368-78933387 | None:intergenic | 60.0% | |
| TCGCGGGAAGCTTCGTCTGA+AGG | + | chr8.1:78933370-78933389 | MS.gene30368:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 78933112 | 78933456 | 78933112 | ID=MS.gene30368 |
| chr8.1 | mRNA | 78933112 | 78933456 | 78933112 | ID=MS.gene30368.t1;Parent=MS.gene30368 |
| chr8.1 | exon | 78933112 | 78933456 | 78933112 | ID=MS.gene30368.t1.exon1;Parent=MS.gene30368.t1 |
| chr8.1 | CDS | 78933112 | 78933456 | 78933112 | ID=cds.MS.gene30368.t1;Parent=MS.gene30368.t1 |
| Gene Sequence |
| Protein sequence |
