Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45567.t1 | RHN52554.1 | 88.3 | 436 | 38 | 3 | 1 | 432 | 1 | 427 | 1.50E-179 | 639 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45567.t1 | O81242 | 48.3 | 377 | 160 | 7 | 1 | 364 | 1 | 355 | 1.5e-74 | 281.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45567.t1 | A0A396HKZ0 | 88.3 | 436 | 38 | 3 | 1 | 432 | 1 | 427 | 1.1e-179 | 639.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene45567 | 0.808019 | 3.80E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene45567 | 0.80053 | 1.38E-48 | -1.69E-46 |
| MS.gene05016 | MS.gene45567 | 0.800848 | 1.19E-48 | -1.69E-46 |
| MS.gene050226 | MS.gene45567 | 0.826482 | 2.65E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene45567 | 0.82605 | 3.36E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene45567 | 0.828902 | 6.94E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene45567 | 0.803101 | 4.09E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene45567 | 0.823246 | 1.54E-53 | -1.69E-46 |
| MS.gene050559 | MS.gene45567 | 0.813931 | 1.99E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene45567 | 0.81895 | 1.50E-52 | -1.69E-46 |
| MS.gene050562 | MS.gene45567 | 0.805824 | 1.11E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene45567 | -0.811693 | 6.16E-51 | -1.69E-46 |
| MS.gene050719 | MS.gene45567 | 0.820177 | 7.87E-53 | -1.69E-46 |
| MS.gene050721 | MS.gene45567 | 0.816502 | 5.35E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene45567 | 0.8126 | 3.91E-51 | -1.69E-46 |
| MS.gene050780 | MS.gene45567 | 0.805941 | 1.05E-49 | -1.69E-46 |
| MS.gene050987 | MS.gene45567 | 0.802527 | 5.38E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene45567 | 0.805685 | 1.18E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene45567 | 0.818418 | 1.98E-52 | -1.69E-46 |
| MS.gene052012 | MS.gene45567 | 0.824815 | 6.58E-54 | -1.69E-46 |
| MS.gene052108 | MS.gene45567 | 0.805649 | 1.21E-49 | -1.69E-46 |
| MS.gene05215 | MS.gene45567 | 0.805308 | 1.42E-49 | -1.69E-46 |
| MS.gene052323 | MS.gene45567 | 0.826764 | 2.27E-54 | -1.69E-46 |
| MS.gene052858 | MS.gene45567 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene052860 | MS.gene45567 | 0.807929 | 3.98E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene45567 | 0.821304 | 4.34E-53 | -1.69E-46 |
| MS.gene053080 | MS.gene45567 | 0.835712 | 1.43E-56 | -1.69E-46 |
| MS.gene053226 | MS.gene45567 | 0.823227 | 1.55E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene45567 | 0.800494 | 1.40E-48 | -1.69E-46 |
| MS.gene054657 | MS.gene45567 | 0.838373 | 2.99E-57 | -1.69E-46 |
| MS.gene054658 | MS.gene45567 | 0.825778 | 3.89E-54 | -1.69E-46 |
| MS.gene054994 | MS.gene45567 | 0.805058 | 1.60E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene45567 | 0.8035 | 3.38E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene45567 | 0.80848 | 3.03E-50 | -1.69E-46 |
| MS.gene055222 | MS.gene45567 | 0.824354 | 8.45E-54 | -1.69E-46 |
| MS.gene055512 | MS.gene45567 | 0.837414 | 5.27E-57 | -1.69E-46 |
| MS.gene055623 | MS.gene45567 | 0.815931 | 7.18E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene45567 | 0.807991 | 3.86E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene45567 | 0.803322 | 3.68E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene45567 | 0.8614 | 1.05E-63 | -1.69E-46 |
| MS.gene056552 | MS.gene45567 | 0.80675 | 7.07E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene45567 | 0.817218 | 3.70E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene45567 | 0.814501 | 1.49E-51 | -1.69E-46 |
| MS.gene057140 | MS.gene45567 | 0.827421 | 1.58E-54 | -1.69E-46 |
| MS.gene057208 | MS.gene45567 | 0.809586 | 1.76E-50 | -1.69E-46 |
| MS.gene057360 | MS.gene45567 | 0.802603 | 5.19E-49 | -1.69E-46 |
| MS.gene057555 | MS.gene45567 | 0.808978 | 2.37E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene45567 | 0.805687 | 1.18E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene45567 | 0.83661 | 8.46E-57 | -1.69E-46 |
| MS.gene057915 | MS.gene45567 | 0.803674 | 3.11E-49 | -1.69E-46 |
| MS.gene05795 | MS.gene45567 | 0.814399 | 1.57E-51 | -1.69E-46 |
| MS.gene058126 | MS.gene45567 | 0.827742 | 1.32E-54 | -1.69E-46 |
| MS.gene058548 | MS.gene45567 | 0.819206 | 1.31E-52 | -1.69E-46 |
| MS.gene058595 | MS.gene45567 | 0.807023 | 6.19E-50 | -1.69E-46 |
| MS.gene059317 | MS.gene45567 | 0.822613 | 2.16E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene45567 | 0.812989 | 3.21E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene45567 | 0.807551 | 4.78E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene45567 | 0.806204 | 9.22E-50 | -1.69E-46 |
| MS.gene05978 | MS.gene45567 | 0.813204 | 2.88E-51 | -1.69E-46 |
| MS.gene05979 | MS.gene45567 | 0.809028 | 2.32E-50 | -1.69E-46 |
| MS.gene059830 | MS.gene45567 | 0.822237 | 2.64E-53 | -1.69E-46 |
| MS.gene060297 | MS.gene45567 | 0.821685 | 3.54E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene45567 | 0.807318 | 5.36E-50 | -1.69E-46 |
| MS.gene060619 | MS.gene45567 | 0.800044 | 1.73E-48 | -1.69E-46 |
| MS.gene060637 | MS.gene45567 | 0.802543 | 5.34E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene45567 | 0.800678 | 1.29E-48 | -1.69E-46 |
| MS.gene060907 | MS.gene45567 | 0.819666 | 1.03E-52 | -1.69E-46 |
| MS.gene060998 | MS.gene45567 | 0.803615 | 3.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45567.t1 | MTR_6g077860 | 93.807 | 436 | 11 | 3 | 1 | 432 | 1 | 424 | 0.0 | 806 |
| MS.gene45567.t1 | MTR_6g077860 | 93.766 | 401 | 10 | 2 | 1 | 397 | 1 | 390 | 0.0 | 749 |
| MS.gene45567.t1 | MTR_6g077860 | 93.516 | 401 | 10 | 3 | 1 | 397 | 1 | 389 | 0.0 | 743 |
| MS.gene45567.t1 | MTR_6g077860 | 93.277 | 357 | 9 | 2 | 1 | 353 | 1 | 346 | 0.0 | 667 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45567.t1 | AT1G48380 | 51.124 | 356 | 140 | 8 | 22 | 364 | 21 | 355 | 8.83e-99 | 300 |
| MS.gene45567.t1 | AT1G48380 | 47.028 | 387 | 140 | 9 | 22 | 364 | 21 | 386 | 4.92e-92 | 283 |
Find 78 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGCGTTCTATGGTTTC+TGG | 0.031621 | 6.4:+52441109 | None:intergenic |
| AATTCCAGTTTGGCTTCTTC+TGG | 0.132448 | 6.4:+52438473 | None:intergenic |
| AAGGGTCGAATGAAGTTATT+TGG | 0.166724 | 6.4:-52439010 | MS.gene45567:CDS |
| AAATATCCTTATAGGCATTT+TGG | 0.171132 | 6.4:-52437496 | MS.gene45567:CDS |
| CAAACTGAACATGACTTTAA+AGG | 0.229485 | 6.4:-52438329 | MS.gene45567:CDS |
| AACCAGCAGGCTGCGACTTC+TGG | 0.236228 | 6.4:+52435814 | None:intergenic |
| CCTGGTTTGTTTGCTCCTAT+TGG | 0.244363 | 6.4:-52440932 | MS.gene45567:CDS |
| GATTGGCGATCTTAAGGATT+TGG | 0.265316 | 6.4:-52440900 | MS.gene45567:CDS |
| ATTTCTTCTGGTGATGATTC+TGG | 0.283304 | 6.4:-52437597 | MS.gene45567:CDS |
| AGAGTCAAGTATCTATTCTT+TGG | 0.286324 | 6.4:+52438974 | None:intergenic |
| ATGAATTGAACTTCTAGTTT+TGG | 0.292180 | 6.4:+52441045 | None:intergenic |
| ACCAGCTCAAATATCCTTAT+AGG | 0.296418 | 6.4:-52437504 | MS.gene45567:CDS |
| TTTGAGTCTCTTGCGTTCTA+TGG | 0.301405 | 6.4:+52441102 | None:intergenic |
| CATCACGCTCTTTCCACCTT+TGG | 0.317633 | 6.4:+52438943 | None:intergenic |
| AAACTGAACATGACTTTAAA+GGG | 0.326016 | 6.4:-52438328 | MS.gene45567:CDS |
| TCCATGGGGAGTTGATCTTT+TGG | 0.326043 | 6.4:+52437154 | None:intergenic |
| ATTGGCGATCTTAAGGATTT+GGG | 0.348552 | 6.4:-52440899 | MS.gene45567:CDS |
| CTTAAAAGACACAACAGAAT+TGG | 0.355540 | 6.4:-52438183 | MS.gene45567:CDS |
| CGGGAAGATTGGCGATCTTA+AGG | 0.360205 | 6.4:-52440906 | MS.gene45567:CDS |
| AGATTTGCTGAAATTTCTTC+TGG | 0.361470 | 6.4:-52437609 | MS.gene45567:CDS |
| TTTCGGATGCATGGTGGATT+GGG | 0.364946 | 6.4:-52438511 | MS.gene45567:CDS |
| ATTCCAGTTTGGCTTCTTCT+GGG | 0.367130 | 6.4:+52438474 | None:intergenic |
| TTTAGGATTCTGACGAAGAT+TGG | 0.368574 | 6.4:-52433604 | MS.gene45567:intron |
| TCTTGCGTTCTATGGTTTCT+GGG | 0.375719 | 6.4:+52441110 | None:intergenic |
| TGATGGAGATTTATTTGAAT+TGG | 0.386109 | 6.4:+52435942 | None:intergenic |
| GGGATGCCAAAATGCCTATA+AGG | 0.391636 | 6.4:+52437490 | None:intergenic |
| CCAATCTTCCCGCCACCAAT+AGG | 0.401409 | 6.4:+52440917 | None:intergenic |
| GTCTCCGGTGACTCTGGCTC+TGG | 0.414618 | 6.4:+52438242 | None:intergenic |
| GAACCCAGAAGAAGCCAAAC+TGG | 0.414656 | 6.4:-52438477 | MS.gene45567:CDS |
| TTCCTTGGGAAATTCCAGTT+TGG | 0.420662 | 6.4:+52438463 | None:intergenic |
| CCTATTGGTGGCGGGAAGAT+TGG | 0.425962 | 6.4:-52440917 | MS.gene45567:CDS |
| CCTAACCTCATACAGTTCCT+TGG | 0.426084 | 6.4:+52438448 | None:intergenic |
| ATCCTCATTATTCAAGAAAG+TGG | 0.428728 | 6.4:-52437071 | MS.gene45567:CDS |
| GTTCTCGCTGACTGGCGAAC+TGG | 0.429471 | 6.4:+52438158 | None:intergenic |
| TTAGGATTCTGACGAAGATT+GGG | 0.441138 | 6.4:-52433603 | MS.gene45567:intron |
| TTCTTCATGTTCAGAAAGGT+CGG | 0.441717 | 6.4:+52437563 | None:intergenic |
| GGCCAGAAGTCGCAGCCTGC+TGG | 0.442610 | 6.4:-52435816 | MS.gene45567:CDS |
| TCCATTGTTGCTAAACATCA+TGG | 0.444208 | 6.4:-52441010 | MS.gene45567:CDS |
| CTAACCTCATACAGTTCCTT+GGG | 0.447574 | 6.4:+52438449 | None:intergenic |
| GGGAAATCGAGGTAAAGAAT+TGG | 0.450558 | 6.4:+52440866 | None:intergenic |
| ATTTCCCAAGGAACTGTATG+AGG | 0.460309 | 6.4:-52438453 | MS.gene45567:intron |
| CACGTTGTTTGAAAATGCTA+AGG | 0.461867 | 6.4:+52441078 | None:intergenic |
| TTCGAAAAGAAAGATTGAAC+TGG | 0.467814 | 6.4:-52435793 | MS.gene45567:intron |
| TTCCACTTTCTTGAATAATG+AGG | 0.470268 | 6.4:+52437069 | None:intergenic |
| AGGGTCGAATGAAGTTATTT+GGG | 0.471456 | 6.4:-52439009 | MS.gene45567:CDS |
| AGAGGTGTCTCCGGTGACTC+TGG | 0.472520 | 6.4:+52438236 | None:intergenic |
| TCTTCATGTTCAGAAAGGTC+GGG | 0.475296 | 6.4:+52437564 | None:intergenic |
| GGTTTCTGGGTTTGTTGCGT+CGG | 0.482601 | 6.4:+52441123 | None:intergenic |
| ATTCAAGAAAGTGGAAGTAA+AGG | 0.493741 | 6.4:-52437062 | MS.gene45567:intron |
| GGTTTGTTTGCTCCTATTGG+TGG | 0.497317 | 6.4:-52440929 | MS.gene45567:CDS |
| TCTTTACCTCGATTTCCCTC+AGG | 0.499443 | 6.4:-52440861 | MS.gene45567:intron |
| AATAAATCTCCATCATCAAA+AGG | 0.500680 | 6.4:-52435934 | MS.gene45567:intron |
| TGTCTGTTGTTGTAGGATGA+AGG | 0.507312 | 6.4:-52435690 | MS.gene45567:intron |
| AGGATGAAGGACCCAAGAAA+AGG | 0.510584 | 6.4:-52435677 | MS.gene45567:CDS |
| GCCTATAAGGATATTTGAGC+TGG | 0.523542 | 6.4:+52437503 | None:intergenic |
| CTTCATGTTCAGAAAGGTCG+GGG | 0.526407 | 6.4:+52437565 | None:intergenic |
| GGATGAAGGACCCAAGAAAA+GGG | 0.530586 | 6.4:-52435676 | MS.gene45567:CDS |
| ACGTCCAGAGCCAGAGTCAC+CGG | 0.540738 | 6.4:-52438246 | MS.gene45567:CDS |
| TCATCTTCAAGAGGTGTCTC+CGG | 0.541255 | 6.4:+52438227 | None:intergenic |
| TGTTTAGCAACAATGGAAGA+AGG | 0.545180 | 6.4:+52441016 | None:intergenic |
| TTGTTTGCTCCTATTGGTGG+CGG | 0.551217 | 6.4:-52440926 | MS.gene45567:CDS |
| ACCATGATGTTTAGCAACAA+TGG | 0.563611 | 6.4:+52441009 | None:intergenic |
| ACAAACCAGGAAACGAGAAA+AGG | 0.564488 | 6.4:+52440945 | None:intergenic |
| TGTTTGCTCCTATTGGTGGC+GGG | 0.569637 | 6.4:-52440925 | MS.gene45567:CDS |
| CCAATAGGAGCAAACAAACC+AGG | 0.575919 | 6.4:+52440932 | None:intergenic |
| ACTCTACAGTTCTCCAAAGG+TGG | 0.579477 | 6.4:-52438956 | MS.gene45567:CDS |
| TCTGATAAATCATCTTCAAG+AGG | 0.593258 | 6.4:+52438218 | None:intergenic |
| GAAAAGGGCAAGAAAGACCA+AGG | 0.609681 | 6.4:-52435661 | MS.gene45567:CDS |
| GCAGCTTCTGTAGTTAATCA+CGG | 0.627408 | 6.4:-52438290 | MS.gene45567:CDS |
| AGCCAAACTGGAATTTCCCA+AGG | 0.637198 | 6.4:-52438465 | MS.gene45567:CDS |
| TCCAAAAGATCAACTCCCCA+TGG | 0.637769 | 6.4:-52437155 | MS.gene45567:CDS |
| AACTGAACATGACTTTAAAG+GGG | 0.647674 | 6.4:-52438327 | MS.gene45567:CDS |
| TGGAAAGAGCGTGATGTGTG+AGG | 0.647978 | 6.4:-52438936 | MS.gene45567:CDS |
| GAACAACCTGAGGGAAATCG+AGG | 0.649397 | 6.4:+52440855 | None:intergenic |
| TTGACTCTACAGTTCTCCAA+AGG | 0.655390 | 6.4:-52438959 | MS.gene45567:CDS |
| ATATTATTAAGAAGTCTCAG+AGG | 0.677231 | 6.4:-52440982 | MS.gene45567:CDS |
| ACTGAACATGACTTTAAAGG+GGG | 0.698718 | 6.4:-52438326 | MS.gene45567:CDS |
| AGTTATTGACATTGACGATG+AGG | 0.704093 | 6.4:-52437185 | MS.gene45567:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 52433595 | 52441191 | 52433595 | ID=MS.gene45567 |
| chr6.4 | mRNA | 52433595 | 52441191 | 52433595 | ID=MS.gene45567.t1;Parent=MS.gene45567 |
| chr6.4 | exon | 52440862 | 52441191 | 52440862 | ID=MS.gene45567.t1.exon1;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52440862 | 52441191 | 52440862 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52438919 | 52439029 | 52438919 | ID=MS.gene45567.t1.exon2;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52438919 | 52439029 | 52438919 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52438454 | 52438540 | 52438454 | ID=MS.gene45567.t1.exon3;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52438454 | 52438540 | 52438454 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52438140 | 52438357 | 52438140 | ID=MS.gene45567.t1.exon4;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52438140 | 52438357 | 52438140 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52437489 | 52437629 | 52437489 | ID=MS.gene45567.t1.exon5;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52437489 | 52437629 | 52437489 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52437063 | 52437225 | 52437063 | ID=MS.gene45567.t1.exon6;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52437063 | 52437225 | 52437063 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52435935 | 52435974 | 52435935 | ID=MS.gene45567.t1.exon7;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52435935 | 52435974 | 52435935 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52435794 | 52435843 | 52435794 | ID=MS.gene45567.t1.exon8;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52435794 | 52435843 | 52435794 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52435649 | 52435697 | 52435649 | ID=MS.gene45567.t1.exon9;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52435649 | 52435697 | 52435649 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52435144 | 52435178 | 52435144 | ID=MS.gene45567.t1.exon10;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52435144 | 52435178 | 52435144 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52433773 | 52433820 | 52433773 | ID=MS.gene45567.t1.exon11;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52433773 | 52433820 | 52433773 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| chr6.4 | exon | 52433595 | 52433621 | 52433595 | ID=MS.gene45567.t1.exon12;Parent=MS.gene45567.t1 |
| chr6.4 | CDS | 52433595 | 52433621 | 52433595 | ID=cds.MS.gene45567.t1;Parent=MS.gene45567.t1 |
| Gene Sequence |
| Protein sequence |
