Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56488.t1 | XP_003607784.1 | 90.1 | 81 | 8 | 0 | 1 | 81 | 1 | 81 | 3.60E-13 | 84 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56488.t1 | G7JHG4 | 90.1 | 81 | 8 | 0 | 1 | 81 | 1 | 81 | 2.6e-13 | 84.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene56488 | 0.811426 | 7.04E-51 | -1.69E-46 |
| MS.gene049151 | MS.gene56488 | 0.820844 | 5.53E-53 | -1.69E-46 |
| MS.gene049153 | MS.gene56488 | 0.820354 | 7.17E-53 | -1.69E-46 |
| MS.gene049346 | MS.gene56488 | 0.816051 | 6.75E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene56488 | 0.812993 | 3.21E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene56488 | 0.85503 | 8.29E-62 | -1.69E-46 |
| MS.gene049910 | MS.gene56488 | 0.843369 | 1.46E-58 | -1.69E-46 |
| MS.gene049915 | MS.gene56488 | 0.814035 | 1.89E-51 | -1.69E-46 |
| MS.gene050397 | MS.gene56488 | 0.838616 | 2.59E-57 | -1.69E-46 |
| MS.gene050406 | MS.gene56488 | 0.82072 | 5.91E-53 | -1.69E-46 |
| MS.gene050426 | MS.gene56488 | -0.848745 | 5.04E-60 | -1.69E-46 |
| MS.gene050464 | MS.gene56488 | 0.83972 | 1.34E-57 | -1.69E-46 |
| MS.gene050529 | MS.gene56488 | 0.836814 | 7.51E-57 | -1.69E-46 |
| MS.gene050623 | MS.gene56488 | -0.82394 | 1.06E-53 | -1.69E-46 |
| MS.gene050642 | MS.gene56488 | 0.847063 | 1.47E-59 | -1.69E-46 |
| MS.gene050670 | MS.gene56488 | 0.801653 | 8.13E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene56488 | 0.814638 | 1.39E-51 | -1.69E-46 |
| MS.gene051535 | MS.gene56488 | 0.810178 | 1.31E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene56488 | 0.817982 | 2.49E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene56488 | 0.867266 | 1.55E-65 | -1.69E-46 |
| MS.gene052011 | MS.gene56488 | -0.806112 | 9.63E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene56488 | 0.900821 | 4.99E-78 | -1.69E-46 |
| MS.gene052135 | MS.gene56488 | 0.80102 | 1.10E-48 | -1.69E-46 |
| MS.gene052178 | MS.gene56488 | 0.831037 | 2.09E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene56488 | 0.833082 | 6.54E-56 | -1.69E-46 |
| MS.gene052310 | MS.gene56488 | 0.814944 | 1.19E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene56488 | 0.869817 | 2.33E-66 | -1.69E-46 |
| MS.gene053073 | MS.gene56488 | 0.829959 | 3.84E-55 | -1.69E-46 |
| MS.gene05316 | MS.gene56488 | 0.82422 | 9.08E-54 | -1.69E-46 |
| MS.gene053494 | MS.gene56488 | 0.831874 | 1.30E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene56488 | 0.843666 | 1.22E-58 | -1.69E-46 |
| MS.gene053836 | MS.gene56488 | 0.847878 | 8.75E-60 | -1.69E-46 |
| MS.gene053928 | MS.gene56488 | 0.807685 | 4.48E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene56488 | 0.800244 | 1.58E-48 | -1.69E-46 |
| MS.gene054349 | MS.gene56488 | 0.858701 | 6.87E-63 | -1.69E-46 |
| MS.gene054379 | MS.gene56488 | 0.804225 | 2.39E-49 | -1.69E-46 |
| MS.gene054542 | MS.gene56488 | 0.815493 | 8.99E-52 | -1.69E-46 |
| MS.gene054930 | MS.gene56488 | 0.835606 | 1.52E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene56488 | 0.829211 | 5.84E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene56488 | 0.821033 | 5.01E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene56488 | 0.854912 | 8.97E-62 | -1.69E-46 |
| MS.gene055701 | MS.gene56488 | 0.813935 | 1.99E-51 | -1.69E-46 |
| MS.gene055859 | MS.gene56488 | 0.814519 | 1.48E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene56488 | 0.809513 | 1.82E-50 | -1.69E-46 |
| MS.gene056447 | MS.gene56488 | 0.817303 | 3.54E-52 | -1.69E-46 |
| MS.gene056612 | MS.gene56488 | 0.814737 | 1.32E-51 | -1.69E-46 |
| MS.gene056659 | MS.gene56488 | 0.804598 | 2.00E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene56488 | -0.810699 | 1.01E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene56488 | 0.864933 | 8.51E-65 | -1.69E-46 |
| MS.gene05702 | MS.gene56488 | 0.853029 | 3.13E-61 | -1.69E-46 |
| MS.gene057396 | MS.gene56488 | 0.824348 | 8.47E-54 | -1.69E-46 |
| MS.gene057448 | MS.gene56488 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene56488 | 0.808071 | 3.71E-50 | -1.69E-46 |
| MS.gene057754 | MS.gene56488 | 0.817353 | 3.45E-52 | -1.69E-46 |
| MS.gene057927 | MS.gene56488 | 0.80261 | 5.17E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene56488 | 0.813375 | 2.64E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene56488 | 0.815138 | 1.08E-51 | -1.69E-46 |
| MS.gene058142 | MS.gene56488 | 0.809162 | 2.17E-50 | -1.69E-46 |
| MS.gene058276 | MS.gene56488 | 0.801426 | 9.06E-49 | -1.69E-46 |
| MS.gene058435 | MS.gene56488 | 0.820204 | 7.76E-53 | -1.69E-46 |
| MS.gene058710 | MS.gene56488 | 0.823052 | 1.70E-53 | -1.69E-46 |
| MS.gene058845 | MS.gene56488 | 0.809864 | 1.53E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene56488 | 0.812467 | 4.18E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene56488 | 0.835805 | 1.35E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene56488 | 0.848201 | 7.13E-60 | -1.69E-46 |
| MS.gene059294 | MS.gene56488 | 0.81582 | 7.60E-52 | -1.69E-46 |
| MS.gene059420 | MS.gene56488 | 0.818926 | 1.52E-52 | -1.69E-46 |
| MS.gene059421 | MS.gene56488 | 0.804252 | 2.36E-49 | -1.69E-46 |
| MS.gene059511 | MS.gene56488 | 0.829342 | 5.43E-55 | -1.69E-46 |
| MS.gene059807 | MS.gene56488 | 0.823154 | 1.61E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene56488 | 0.82846 | 8.88E-55 | -1.69E-46 |
| MS.gene059894 | MS.gene56488 | 0.866822 | 2.15E-65 | -1.69E-46 |
| MS.gene060084 | MS.gene56488 | 0.806788 | 6.94E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene56488 | 0.801939 | 7.11E-49 | -1.69E-46 |
| MS.gene060495 | MS.gene56488 | 0.806468 | 8.11E-50 | -1.69E-46 |
| MS.gene060940 | MS.gene56488 | 0.84424 | 8.55E-59 | -1.69E-46 |
| MS.gene060942 | MS.gene56488 | 0.818373 | 2.03E-52 | -1.69E-46 |
| MS.gene060943 | MS.gene56488 | 0.82373 | 1.18E-53 | -1.69E-46 |
| MS.gene061002 | MS.gene56488 | 0.842828 | 2.04E-58 | -1.69E-46 |
| MS.gene061049 | MS.gene56488 | 0.869394 | 3.20E-66 | -1.69E-46 |
| MS.gene061154 | MS.gene56488 | 0.818092 | 2.35E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene56488 | 0.858027 | 1.09E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56488.t1 | MTR_4g082900 | 83.951 | 81 | 13 | 0 | 1 | 81 | 1 | 81 | 6.92e-36 | 116 |
| MS.gene56488.t1 | MTR_4g082883 | 58.108 | 74 | 31 | 0 | 8 | 81 | 26 | 99 | 8.39e-21 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGGACCTAACGATCTTT+TGG | 0.317659 | 4.4:-30135166 | MS.gene56488:CDS |
| TTTGCCAATAGGATCTGGTT+TGG | 0.364938 | 4.4:+30134999 | None:intergenic |
| TTGCCAATAGGATCTGGTTT+GGG | 0.376061 | 4.4:+30135000 | None:intergenic |
| AGCATCATCAACCGCACCTT+TGG | 0.404914 | 4.4:+30134975 | None:intergenic |
| GTACTTGCCAAAAGATCGTT+AGG | 0.439536 | 4.4:+30135159 | None:intergenic |
| AAACCCAAACCAGATCCTAT+TGG | 0.473112 | 4.4:-30135003 | MS.gene56488:CDS |
| GATGATGCTACAAAGTCTGC+TGG | 0.547087 | 4.4:-30134961 | MS.gene56488:CDS |
| ACTGTAGGAAATCTTGCAGC+TGG | 0.550456 | 4.4:-30135108 | MS.gene56488:CDS |
| GCTGGTGTTCATAAGACTGT+AGG | 0.573068 | 4.4:-30135057 | MS.gene56488:CDS |
| GCATCAGCACAGAAAACTGT+AGG | 0.588939 | 4.4:-30135123 | MS.gene56488:CDS |
| GTAGGAAATCTTGCAGCTGG+TGG | 0.593110 | 4.4:-30135105 | MS.gene56488:CDS |
| GATCCTATTGGCAAAACCAA+AGG | 0.604323 | 4.4:-30134991 | MS.gene56488:CDS |
| AATGTGGGAAATGCTGCAGC+TGG | 0.619200 | 4.4:-30135075 | MS.gene56488:CDS |
| TATTGGCAAAACCAAAGGTG+CGG | 0.679241 | 4.4:-30134986 | MS.gene56488:CDS |
| GATGCTACAAAGTCTGCTGG+AGG | 0.702868 | 4.4:-30134958 | MS.gene56488:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTACAAAATCAGCAGCGT+TGG | + | chr4.4:30135093-30135112 | None:intergenic | 35.0% |
| AAACCCAAACCAGATCCTAT+TGG | - | chr4.4:30135110-30135129 | MS.gene56488:CDS | 40.0% | |
| GATCCTATTGGCAAAACCAA+AGG | - | chr4.4:30135122-30135141 | MS.gene56488:CDS | 40.0% | |
| GTACTTGCCAAAAGATCGTT+AGG | + | chr4.4:30134957-30134976 | None:intergenic | 40.0% | |
| TATTGGCAAAACCAAAGGTG+CGG | - | chr4.4:30135127-30135146 | MS.gene56488:CDS | 40.0% | |
| TTTGCCAATAGGATCTGGTT+TGG | + | chr4.4:30135117-30135136 | None:intergenic | 40.0% | |
| ! | CAGCATTTCCCACATTTTTC+TGG | + | chr4.4:30135034-30135053 | None:intergenic | 40.0% |
| ! | CGATCTTTTGGCAAGTACAA+AGG | - | chr4.4:30134959-30134978 | MS.gene56488:CDS | 40.0% |
| ! | TTGCCAATAGGATCTGGTTT+GGG | + | chr4.4:30135116-30135135 | None:intergenic | 40.0% |
| !!! | TTGGTTTTGCCAATAGGATC+TGG | + | chr4.4:30135122-30135141 | None:intergenic | 40.0% |
| ACTGTAGGAAATCTTGCAGC+TGG | - | chr4.4:30135005-30135024 | MS.gene56488:CDS | 45.0% | |
| GATGATGCTACAAAGTCTGC+TGG | - | chr4.4:30135152-30135171 | MS.gene56488:CDS | 45.0% | |
| GCATCAGCACAGAAAACTGT+AGG | - | chr4.4:30134990-30135009 | MS.gene56488:CDS | 45.0% | |
| GCTGGTGTTCATAAGACTGT+AGG | - | chr4.4:30135056-30135075 | MS.gene56488:CDS | 45.0% | |
| !!! | GCACCTTTGGTTTTGCCAAT+AGG | + | chr4.4:30135128-30135147 | None:intergenic | 45.0% |
| AATGTGGGAAATGCTGCAGC+TGG | - | chr4.4:30135038-30135057 | MS.gene56488:CDS | 50.0% | |
| AGCATCATCAACCGCACCTT+TGG | + | chr4.4:30135141-30135160 | None:intergenic | 50.0% | |
| AGCTGGTGGCCAGAAAAATG+TGG | - | chr4.4:30135022-30135041 | MS.gene56488:CDS | 50.0% | |
| GATGCTACAAAGTCTGCTGG+AGG | - | chr4.4:30135155-30135174 | MS.gene56488:CDS | 50.0% | |
| GCTGGTGGCCAGAAAAATGT+GGG | - | chr4.4:30135023-30135042 | MS.gene56488:CDS | 50.0% | |
| GTAGGAAATCTTGCAGCTGG+TGG | - | chr4.4:30135008-30135027 | MS.gene56488:CDS | 50.0% | |
| ! | GGCTGGACCTAACGATCTTT+TGG | - | chr4.4:30134947-30134966 | MS.gene56488:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 30134945 | 30135190 | 30134945 | ID=MS.gene56488 |
| chr4.4 | mRNA | 30134945 | 30135190 | 30134945 | ID=MS.gene56488.t1;Parent=MS.gene56488 |
| chr4.4 | exon | 30134945 | 30135190 | 30134945 | ID=MS.gene56488.t1.exon1;Parent=MS.gene56488.t1 |
| chr4.4 | CDS | 30134945 | 30135190 | 30134945 | ID=cds.MS.gene56488.t1;Parent=MS.gene56488.t1 |
| Gene Sequence |
| Protein sequence |
