Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68204.t1 | XP_024635707.1 | 88.3 | 264 | 30 | 1 | 1 | 264 | 1 | 263 | 2.10E-118 | 435.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68204.t1 | G7J762 | 88.3 | 264 | 30 | 1 | 1 | 264 | 1 | 263 | 1.5e-118 | 435.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene68204 | 0.878721 | 2.23E-69 | -1.69E-46 |
| MS.gene049159 | MS.gene68204 | 0.810228 | 1.28E-50 | -1.69E-46 |
| MS.gene04918 | MS.gene68204 | 0.810709 | 1.01E-50 | -1.69E-46 |
| MS.gene04920 | MS.gene68204 | 0.804407 | 2.19E-49 | -1.69E-46 |
| MS.gene049227 | MS.gene68204 | 0.824358 | 8.43E-54 | -1.69E-46 |
| MS.gene049254 | MS.gene68204 | 0.835269 | 1.85E-56 | -1.69E-46 |
| MS.gene049350 | MS.gene68204 | 0.83276 | 7.86E-56 | -1.69E-46 |
| MS.gene049368 | MS.gene68204 | 0.823211 | 1.56E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene68204 | 0.823593 | 1.27E-53 | -1.69E-46 |
| MS.gene049711 | MS.gene68204 | 0.830989 | 2.15E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene68204 | 0.83698 | 6.81E-57 | -1.69E-46 |
| MS.gene049983 | MS.gene68204 | 0.840586 | 7.95E-58 | -1.69E-46 |
| MS.gene050551 | MS.gene68204 | 0.84547 | 3.98E-59 | -1.69E-46 |
| MS.gene050646 | MS.gene68204 | 0.809034 | 2.31E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene68204 | 0.806964 | 6.37E-50 | -1.69E-46 |
| MS.gene050657 | MS.gene68204 | 0.815081 | 1.11E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene68204 | 0.801829 | 7.49E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene68204 | 0.804054 | 2.60E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene68204 | 0.804497 | 2.10E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene68204 | 0.816993 | 4.15E-52 | -1.69E-46 |
| MS.gene051700 | MS.gene68204 | 0.80937 | 1.96E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene68204 | 0.803803 | 2.93E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene68204 | 0.835195 | 1.93E-56 | -1.69E-46 |
| MS.gene051852 | MS.gene68204 | 0.814534 | 1.47E-51 | -1.69E-46 |
| MS.gene052037 | MS.gene68204 | 0.817241 | 3.65E-52 | -1.69E-46 |
| MS.gene052307 | MS.gene68204 | 0.860595 | 1.85E-63 | -1.69E-46 |
| MS.gene052463 | MS.gene68204 | 0.863677 | 2.10E-64 | -1.69E-46 |
| MS.gene052516 | MS.gene68204 | 0.842649 | 2.27E-58 | -1.69E-46 |
| MS.gene052518 | MS.gene68204 | 0.821297 | 4.35E-53 | -1.69E-46 |
| MS.gene052609 | MS.gene68204 | 0.834219 | 3.40E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene68204 | 0.807537 | 4.82E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene68204 | 0.80691 | 6.54E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene68204 | 0.810952 | 8.92E-51 | -1.69E-46 |
| MS.gene053079 | MS.gene68204 | 0.832718 | 8.06E-56 | -1.69E-46 |
| MS.gene053144 | MS.gene68204 | 0.826902 | 2.10E-54 | -1.69E-46 |
| MS.gene053164 | MS.gene68204 | 0.803453 | 3.46E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene68204 | 0.822245 | 2.63E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene68204 | 0.807222 | 5.62E-50 | -1.69E-46 |
| MS.gene054489 | MS.gene68204 | 0.802049 | 6.75E-49 | -1.69E-46 |
| MS.gene05454 | MS.gene68204 | 0.800759 | 1.24E-48 | -1.69E-46 |
| MS.gene054584 | MS.gene68204 | 0.822959 | 1.79E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene68204 | 0.853934 | 1.72E-61 | -1.69E-46 |
| MS.gene054641 | MS.gene68204 | 0.818325 | 2.08E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene68204 | 0.830489 | 2.85E-55 | -1.69E-46 |
| MS.gene054775 | MS.gene68204 | 0.808112 | 3.64E-50 | -1.69E-46 |
| MS.gene054991 | MS.gene68204 | 0.828066 | 1.10E-54 | -1.69E-46 |
| MS.gene055310 | MS.gene68204 | 0.800451 | 1.43E-48 | -1.69E-46 |
| MS.gene055465 | MS.gene68204 | 0.82833 | 9.55E-55 | -1.69E-46 |
| MS.gene055466 | MS.gene68204 | 0.820466 | 6.76E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene68204 | 0.817581 | 3.06E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene68204 | 0.816663 | 4.92E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene68204 | 0.873879 | 1.04E-67 | -1.69E-46 |
| MS.gene055580 | MS.gene68204 | -0.82254 | 2.24E-53 | -1.69E-46 |
| MS.gene055864 | MS.gene68204 | 0.828956 | 6.74E-55 | -1.69E-46 |
| MS.gene056043 | MS.gene68204 | -0.820541 | 6.50E-53 | -1.69E-46 |
| MS.gene056050 | MS.gene68204 | 0.812177 | 4.83E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene68204 | 0.814449 | 1.53E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene68204 | 0.806291 | 8.83E-50 | -1.69E-46 |
| MS.gene056308 | MS.gene68204 | 0.827995 | 1.15E-54 | -1.69E-46 |
| MS.gene056390 | MS.gene68204 | 0.803989 | 2.68E-49 | -1.69E-46 |
| MS.gene056507 | MS.gene68204 | 0.815591 | 8.55E-52 | -1.69E-46 |
| MS.gene056538 | MS.gene68204 | 0.822166 | 2.74E-53 | -1.69E-46 |
| MS.gene056595 | MS.gene68204 | 0.807451 | 5.02E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene68204 | 0.823652 | 1.23E-53 | -1.69E-46 |
| MS.gene05686 | MS.gene68204 | 0.849965 | 2.30E-60 | -1.69E-46 |
| MS.gene05690 | MS.gene68204 | 0.815311 | 9.87E-52 | -1.69E-46 |
| MS.gene057034 | MS.gene68204 | 0.814729 | 1.33E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene68204 | 0.830172 | 3.41E-55 | -1.69E-46 |
| MS.gene057132 | MS.gene68204 | 0.845329 | 4.35E-59 | -1.69E-46 |
| MS.gene057915 | MS.gene68204 | 0.825488 | 4.56E-54 | -1.69E-46 |
| MS.gene058010 | MS.gene68204 | 0.808975 | 2.38E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene68204 | 0.80165 | 8.15E-49 | -1.69E-46 |
| MS.gene058339 | MS.gene68204 | 0.800837 | 1.20E-48 | -1.69E-46 |
| MS.gene058427 | MS.gene68204 | 0.8062 | 9.23E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene68204 | 0.823283 | 1.51E-53 | -1.69E-46 |
| MS.gene058668 | MS.gene68204 | 0.802763 | 4.81E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene68204 | 0.819313 | 1.24E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene68204 | 0.809982 | 1.45E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene68204 | 0.810209 | 1.29E-50 | -1.69E-46 |
| MS.gene05897 | MS.gene68204 | 0.804951 | 1.69E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene68204 | 0.824271 | 8.84E-54 | -1.69E-46 |
| MS.gene059570 | MS.gene68204 | 0.848054 | 7.83E-60 | -1.69E-46 |
| MS.gene059625 | MS.gene68204 | 0.830129 | 3.49E-55 | -1.69E-46 |
| MS.gene059774 | MS.gene68204 | 0.807975 | 3.89E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene68204 | 0.809729 | 1.64E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene68204 | 0.816259 | 6.06E-52 | -1.69E-46 |
| MS.gene060435 | MS.gene68204 | 0.805534 | 1.27E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene68204 | 0.820588 | 6.34E-53 | -1.69E-46 |
| MS.gene060697 | MS.gene68204 | 0.804771 | 1.84E-49 | -1.69E-46 |
| MS.gene060785 | MS.gene68204 | 0.830724 | 2.50E-55 | -1.69E-46 |
| MS.gene060932 | MS.gene68204 | 0.826086 | 3.29E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene68204 | 0.811397 | 7.15E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene68204 | 0.829567 | 4.79E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene68204 | 0.83989 | 1.21E-57 | -1.69E-46 |
| MS.gene061100 | MS.gene68204 | 0.857923 | 1.17E-62 | -1.69E-46 |
| MS.gene061188 | MS.gene68204 | 0.85752 | 1.54E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68204.t1 | MTR_3g117050 | 92.424 | 264 | 19 | 1 | 1 | 264 | 1 | 263 | 2.62e-174 | 481 |
| MS.gene68204.t1 | MTR_4g070660 | 41.603 | 262 | 98 | 13 | 3 | 254 | 5 | 221 | 7.83e-32 | 117 |
| MS.gene68204.t1 | MTR_4g070640 | 39.370 | 254 | 92 | 10 | 10 | 254 | 9 | 209 | 1.89e-27 | 105 |
| MS.gene68204.t1 | MTR_4g070630 | 36.620 | 213 | 78 | 8 | 49 | 253 | 2 | 165 | 3.27e-25 | 98.6 |
| MS.gene68204.t1 | MTR_8g077085 | 30.612 | 245 | 143 | 10 | 13 | 255 | 5 | 224 | 4.39e-21 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68204.t1 | AT5G66440 | 27.473 | 273 | 147 | 7 | 1 | 256 | 1 | 239 | 1.36e-21 | 91.3 |
| MS.gene68204.t1 | AT4G34560 | 31.034 | 203 | 92 | 7 | 64 | 263 | 63 | 220 | 3.90e-15 | 72.8 |
| MS.gene68204.t1 | AT2G21560 | 26.740 | 273 | 172 | 11 | 1 | 254 | 1 | 264 | 5.87e-13 | 67.8 |
Find 29 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGATTAGCTAGGAATAAA+TGG | 0.252040 | 3.1:-92268636 | None:intergenic |
| AATGAAGTTGGAAGAGAAAC+AGG | 0.271425 | 3.1:+92268331 | MS.gene68204:CDS |
| TCGTTGCTCATTCCATCTTC+AGG | 0.330037 | 3.1:-92268545 | None:intergenic |
| GAAAGATTGCTAATTTGTTT+CGG | 0.382950 | 3.1:+92267922 | MS.gene68204:CDS |
| GTGATAACGATAAGAGTAAG+AGG | 0.395005 | 3.1:+92268477 | MS.gene68204:CDS |
| ATCACACTCATTGCTCAATC+TGG | 0.403087 | 3.1:+92268089 | MS.gene68204:CDS |
| TCAAAGCCAATGGTAGTTGT+TGG | 0.417819 | 3.1:-92267992 | None:intergenic |
| TGGAAGTGAAGGGATATAGA+AGG | 0.441385 | 3.1:+92268375 | MS.gene68204:CDS |
| AAAGTTGAGAACAACGTAAA+GGG | 0.443322 | 3.1:+92268236 | MS.gene68204:CDS |
| AGATGATAATAGAATGAAGT+TGG | 0.450200 | 3.1:+92268319 | MS.gene68204:CDS |
| TTCATTCCAACAACTACCAT+TGG | 0.464826 | 3.1:+92267986 | MS.gene68204:CDS |
| AGTTTATTATATCCTGAAGA+TGG | 0.470634 | 3.1:+92268533 | MS.gene68204:CDS |
| AGAGATGGGTTTGGAAGTGA+AGG | 0.496963 | 3.1:+92268364 | MS.gene68204:CDS |
| GGGATATAGAAGGTGTCAAT+CGG | 0.514951 | 3.1:+92268385 | MS.gene68204:CDS |
| CTTGAGTTCTTCAAAGCCAA+TGG | 0.531388 | 3.1:-92268002 | None:intergenic |
| AAAAGTTGAGAACAACGTAA+AGG | 0.543362 | 3.1:+92268235 | MS.gene68204:CDS |
| TAGAATAAACGATGGATGCA+TGG | 0.556637 | 3.1:+92268262 | MS.gene68204:CDS |
| ATCGGAAATTGAGTTGGTGA+GGG | 0.576160 | 3.1:+92268403 | MS.gene68204:CDS |
| AACATGTATCAGAAAATCCA+AGG | 0.585434 | 3.1:+92268188 | MS.gene68204:CDS |
| AGGATCAAAGAGTGCTGCAA+AGG | 0.598659 | 3.1:+92268447 | MS.gene68204:CDS |
| AATCGGAAATTGAGTTGGTG+AGG | 0.606720 | 3.1:+92268402 | MS.gene68204:CDS |
| GTGTCAATCGGAAATTGAGT+TGG | 0.619603 | 3.1:+92268397 | MS.gene68204:CDS |
| TCTTGCAATAAATGCCTCCA+CGG | 0.627372 | 3.1:-92268582 | None:intergenic |
| TGTGAATAGTGATGATGTGA+AGG | 0.638060 | 3.1:+92268427 | MS.gene68204:CDS |
| GAGATGGGTTTGGAAGTGAA+GGG | 0.651077 | 3.1:+92268365 | MS.gene68204:CDS |
| TCGGAAATTGAGTTGGTGAG+GGG | 0.662410 | 3.1:+92268404 | MS.gene68204:CDS |
| CGAATTTCGTCGCACCGTGG+AGG | 0.663215 | 3.1:+92268568 | MS.gene68204:CDS |
| AAGGGTCGTAGAATAAACGA+TGG | 0.676337 | 3.1:+92268254 | MS.gene68204:CDS |
| CGACGAATTTCGTCGCACCG+TGG | 0.721139 | 3.1:+92268565 | MS.gene68204:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGATGATAATAGAATGAAGT+TGG | + | chr3.1:92268319-92268338 | MS.gene68204:CDS | 25.0% |
| ! | AGTTTATTATATCCTGAAGA+TGG | + | chr3.1:92268533-92268552 | MS.gene68204:CDS | 25.0% |
| !!! | GAAAGATTGCTAATTTGTTT+CGG | + | chr3.1:92267922-92267941 | MS.gene68204:CDS | 25.0% |
| !!! | TTGTGTTGATTTTGATTTCA+AGG | + | chr3.1:92267961-92267980 | MS.gene68204:CDS | 25.0% |
| AAAAGTTGAGAACAACGTAA+AGG | + | chr3.1:92268235-92268254 | MS.gene68204:CDS | 30.0% | |
| AAAGTTGAGAACAACGTAAA+GGG | + | chr3.1:92268236-92268255 | MS.gene68204:CDS | 30.0% | |
| AACATGTATCAGAAAATCCA+AGG | + | chr3.1:92268188-92268207 | MS.gene68204:CDS | 30.0% | |
| ! | AACTCAAGTGAATATTTTCG+AGG | + | chr3.1:92268017-92268036 | MS.gene68204:CDS | 30.0% |
| !! | ACGATGTTTTTGAACTTTGT+TGG | - | chr3.1:92267895-92267914 | None:intergenic | 30.0% |
| !!! | GTTTTTTCTGATCAAAACCT+TGG | - | chr3.1:92268208-92268227 | None:intergenic | 30.0% |
| AAAAACAAAACCAGGACCAA+CGG | - | chr3.1:92268048-92268067 | None:intergenic | 35.0% | |
| AATGAAGTTGGAAGAGAAAC+AGG | + | chr3.1:92268331-92268350 | MS.gene68204:CDS | 35.0% | |
| ACAGGAGATAAAAACAGAGA+TGG | + | chr3.1:92268349-92268368 | MS.gene68204:CDS | 35.0% | |
| CAGGAGATAAAAACAGAGAT+GGG | + | chr3.1:92268350-92268369 | MS.gene68204:CDS | 35.0% | |
| CCAACAAGAAAAACAAAACC+AGG | - | chr3.1:92268056-92268075 | None:intergenic | 35.0% | |
| TAGAATAAACGATGGATGCA+TGG | + | chr3.1:92268262-92268281 | MS.gene68204:CDS | 35.0% | |
| TTCATTCCAACAACTACCAT+TGG | + | chr3.1:92267986-92268005 | MS.gene68204:CDS | 35.0% | |
| ! | GATAAAAACAGAGATGGGTT+TGG | + | chr3.1:92268355-92268374 | MS.gene68204:CDS | 35.0% |
| ! | GTGATAACGATAAGAGTAAG+AGG | + | chr3.1:92268477-92268496 | MS.gene68204:CDS | 35.0% |
| ! | TGTGAATAGTGATGATGTGA+AGG | + | chr3.1:92268427-92268446 | MS.gene68204:CDS | 35.0% |
| !!! | CCTGGTTTTGTTTTTCTTGT+TGG | + | chr3.1:92268053-92268072 | MS.gene68204:CDS | 35.0% |
| AAGGGTCGTAGAATAAACGA+TGG | + | chr3.1:92268254-92268273 | MS.gene68204:CDS | 40.0% | |
| ATCACACTCATTGCTCAATC+TGG | + | chr3.1:92268089-92268108 | MS.gene68204:CDS | 40.0% | |
| CTTGAGTTCTTCAAAGCCAA+TGG | - | chr3.1:92268005-92268024 | None:intergenic | 40.0% | |
| GTGTCAATCGGAAATTGAGT+TGG | + | chr3.1:92268397-92268416 | MS.gene68204:CDS | 40.0% | |
| TCAAAGCCAATGGTAGTTGT+TGG | - | chr3.1:92267995-92268014 | None:intergenic | 40.0% | |
| TCTTGCAATAAATGCCTCCA+CGG | - | chr3.1:92268585-92268604 | None:intergenic | 40.0% | |
| TGGAAGTGAAGGGATATAGA+AGG | + | chr3.1:92268375-92268394 | MS.gene68204:CDS | 40.0% | |
| !! | AATCGGAAATTGAGTTGGTG+AGG | + | chr3.1:92268402-92268421 | MS.gene68204:CDS | 40.0% |
| !! | ATCGGAAATTGAGTTGGTGA+GGG | + | chr3.1:92268403-92268422 | MS.gene68204:CDS | 40.0% |
| !! | GGGATATAGAAGGTGTCAAT+CGG | + | chr3.1:92268385-92268404 | MS.gene68204:CDS | 40.0% |
| !! | TATTTTCGAGGTTTTGCCGT+TGG | + | chr3.1:92268029-92268048 | MS.gene68204:CDS | 40.0% |
| TCGTTGCTCATTCCATCTTC+AGG | - | chr3.1:92268548-92268567 | None:intergenic | 45.0% | |
| ! | AGAGATGGGTTTGGAAGTGA+AGG | + | chr3.1:92268364-92268383 | MS.gene68204:CDS | 45.0% |
| ! | GAGATGGGTTTGGAAGTGAA+GGG | + | chr3.1:92268365-92268384 | MS.gene68204:CDS | 45.0% |
| !! | AGGATCAAAGAGTGCTGCAA+AGG | + | chr3.1:92268447-92268466 | MS.gene68204:CDS | 45.0% |
| !! | TCGGAAATTGAGTTGGTGAG+GGG | + | chr3.1:92268404-92268423 | MS.gene68204:CDS | 45.0% |
| CGAATTTCGTCGCACCGTGG+AGG | + | chr3.1:92268568-92268587 | MS.gene68204:CDS | 60.0% | |
| CGACGAATTTCGTCGCACCG+TGG | + | chr3.1:92268565-92268584 | MS.gene68204:CDS | 60.0% | |
| ! | CGAGGTTTTGCCGTTGGTCC+TGG | + | chr3.1:92268035-92268054 | MS.gene68204:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 92267852 | 92268649 | 92267852 | ID=MS.gene68204 |
| chr3.1 | mRNA | 92267852 | 92268649 | 92267852 | ID=MS.gene68204.t1;Parent=MS.gene68204 |
| chr3.1 | exon | 92267852 | 92268649 | 92267852 | ID=MS.gene68204.t1.exon1;Parent=MS.gene68204.t1 |
| chr3.1 | CDS | 92267852 | 92268649 | 92267852 | ID=cds.MS.gene68204.t1;Parent=MS.gene68204.t1 |
| Gene Sequence |
| Protein sequence |
