AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72286


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72286.t1 MTR_4g102220 96.889 225 7 0 1 225 1 225 9.53e-160 441
MS.gene72286.t1 MTR_5g011800 59.574 235 79 6 1 225 1 229 2.59e-86 255
MS.gene72286.t1 MTR_5g011820 57.021 235 85 7 1 225 4 232 2.77e-81 243
MS.gene72286.t1 MTR_4g024370 52.521 238 92 8 1 224 1 231 3.02e-72 219
MS.gene72286.t1 MTR_4g024370 52.521 238 92 8 1 224 1 231 3.02e-72 219
MS.gene72286.t1 MTR_4g023950 32.143 224 128 8 5 224 4 207 3.96e-23 93.2
MS.gene72286.t1 MTR_1g076800 31.556 225 132 7 5 224 4 211 9.67e-23 92.4
MS.gene72286.t1 MTR_6g060390 30.837 227 132 10 5 224 4 212 1.11e-22 92.0
MS.gene72286.t1 MTR_6g060440 28.444 225 138 7 5 224 4 210 2.21e-22 91.3
MS.gene72286.t1 MTR_7g117380 31.466 232 134 11 5 224 3 221 4.45e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72286.t1 AT2G45360 54.867 226 90 4 1 225 1 215 6.92e-72 218
MS.gene72286.t1 AT3G60780 51.316 228 98 5 1 225 1 218 4.50e-69 211
MS.gene72286.t1 AT1G62840 49.569 232 102 5 1 225 1 224 1.00e-60 190
MS.gene72286.t1 AT1G12320 47.788 226 99 5 1 223 1 210 1.50e-51 166
MS.gene72286.t1 AT5G62280 29.487 234 138 10 5 224 4 224 4.10e-16 75.1

Find 44 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGATTGTGAGGGAATTA+AGG 0.232972 8.2:+21400470 MS.gene72286:CDS
TAAGGTCACGTCGTGTTGTT+AGG 0.340628 8.2:+21400616 MS.gene72286:CDS
TAGCACATGTTTCAGCTGTT+GGG 0.366973 8.2:+21400673 MS.gene72286:CDS
GGTGGCTAAGTTAAGTGTTA+AGG 0.386848 8.2:+21400512 MS.gene72286:CDS
TTGTTGAGGAAGGAGGATTA+AGG 0.425189 8.2:+21400598 MS.gene72286:CDS
AGGTCGGTGTTTCTTCCTGT+TGG 0.430254 8.2:+21400636 MS.gene72286:CDS
TGCGTTCCATCCTGCTGCCA+TGG 0.440434 8.2:-21400220 None:intergenic
AAGCATGTTGATCAACGGTC+AGG 0.453278 8.2:+21400747 MS.gene72286:CDS
ATGTCAAGTCCCTTCCCAAC+AGG 0.460059 8.2:-21400651 None:intergenic
GGGAATTAAGGATTTGATGA+AGG 0.460412 8.2:+21400482 MS.gene72286:CDS
GGTCGGTGTTTCTTCCTGTT+GGG 0.463683 8.2:+21400637 MS.gene72286:CDS
GTGGCTAAGTTAAGTGTTAA+GGG 0.463703 8.2:+21400513 MS.gene72286:CDS
GTGTTTCTTCCTGTTGGGAA+GGG 0.468303 8.2:+21400642 MS.gene72286:CDS
ATAGCACATGTTTCAGCTGT+TGG 0.469433 8.2:+21400672 MS.gene72286:CDS
AAGAGAAAATGAAACTTGTC+TGG 0.470997 8.2:+21399915 None:intergenic
GGTGTTTCTTCCTGTTGGGA+AGG 0.479058 8.2:+21400641 MS.gene72286:CDS
CATTGAAGAGATAGATTTCG+TGG 0.482218 8.2:+21400446 MS.gene72286:CDS
CTGATTTCAGCCATGGCAGC+AGG 0.486556 8.2:+21400210 MS.gene72286:CDS
GATATTATTGTTGAGGAAGG+AGG 0.500593 8.2:+21400591 MS.gene72286:CDS
TGAAATCAGCTCCGCCATGC+CGG 0.504005 8.2:-21400196 None:intergenic
ACATCAACAGAAATCATTGT+AGG 0.506787 8.2:+21400396 MS.gene72286:CDS
GGTGTAATCGAAACAAGCTT+AGG 0.508623 8.2:+21400273 MS.gene72286:CDS
AGCACATGTTTCAGCTGTTG+GGG 0.510606 8.2:+21400674 MS.gene72286:CDS
GAGGATATTATTGTTGAGGA+AGG 0.517535 8.2:+21400588 MS.gene72286:CDS
GGCGGAGCTGATTTCAGCCA+TGG 0.524952 8.2:+21400203 MS.gene72286:CDS
GGAAAAGATGGTCATGGTCG+TGG 0.531446 8.2:+21400705 MS.gene72286:CDS
GTCACGTCGTGTTGTTAGGT+CGG 0.536069 8.2:+21400620 MS.gene72286:CDS
AGCATGTTGATCAACGGTCA+GGG 0.549561 8.2:+21400748 MS.gene72286:CDS
ATGGGAGGATATTATTGTTG+AGG 0.550906 8.2:+21400584 MS.gene72286:CDS
TTTCGTGGTGGTAGATTGTG+AGG 0.551539 8.2:+21400461 MS.gene72286:CDS
GTCGTGGTGGTAGTAAGAGA+TGG 0.579545 8.2:+21400721 MS.gene72286:CDS
TTTCAGCCATGGCAGCAGGA+TGG 0.596118 8.2:+21400214 MS.gene72286:CDS
ATAGTGGAAACATGGTCAGA+AGG 0.600619 8.2:+21400249 MS.gene72286:CDS
TTCGTGGTGGTAGATTGTGA+GGG 0.604870 8.2:+21400462 MS.gene72286:CDS
GGGAGGTGCATGTTATTAGA+CGG 0.626486 8.2:+21400769 MS.gene72286:CDS
GCATGTTGATCAACGGTCAG+GGG 0.633202 8.2:+21400749 MS.gene72286:CDS
GGATTAAGCATGTTGATCAA+CGG 0.643256 8.2:+21400742 MS.gene72286:CDS
TGTTGATCAACGGTCAGGGG+AGG 0.647427 8.2:+21400752 MS.gene72286:CDS
GTGGAAACATGGTCAGAAGG+AGG 0.667051 8.2:+21400252 MS.gene72286:CDS
AAAGATGGTCATGGTCGTGG+TGG 0.675881 8.2:+21400708 MS.gene72286:CDS
AATTTGGGAAAAGATGGTCA+TGG 0.694637 8.2:+21400699 MS.gene72286:CDS
GCACATGTTTCAGCTGTTGG+GGG 0.717069 8.2:+21400675 MS.gene72286:CDS
TGAAGAGATAGATTTCGTGG+TGG 0.721855 8.2:+21400449 MS.gene72286:CDS
TTTACGTGGATCCGGCATGG+CGG 0.735740 8.2:+21400185 MS.gene72286:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTATTCAAGATTAATA+TGG - chr8.2:21400042-21400061 None:intergenic 10.0%
!! ATTATTATTAACAACAGTAA+CGG - chr8.2:21400140-21400159 None:intergenic 15.0%
!!! TATGTAAGAATGTTAATTTT+AGG + chr8.2:21400547-21400566 MS.gene72286:CDS 15.0%
!! ACTAGAGTACTCTAAAAAAA+TGG + chr8.2:21400362-21400381 MS.gene72286:CDS 25.0%
!! CTAGAGTACTCTAAAAAAAT+GGG + chr8.2:21400363-21400382 MS.gene72286:CDS 25.0%
!!! AAGAATGTTAATTTTAGGAG+TGG + chr8.2:21400552-21400571 MS.gene72286:CDS 25.0%
!!! AGAATGTTAATTTTAGGAGT+GGG + chr8.2:21400553-21400572 MS.gene72286:CDS 25.0%
ACATCAACAGAAATCATTGT+AGG + chr8.2:21400396-21400415 MS.gene72286:CDS 30.0%
TCTAAAAAAATGGGTGAACA+AGG + chr8.2:21400372-21400391 MS.gene72286:CDS 30.0%
! ATAGCAAAAAAGCACACAAA+TGG + chr8.2:21400303-21400322 MS.gene72286:CDS 30.0%
! TAGCAAAAAAGCACACAAAT+GGG + chr8.2:21400304-21400323 MS.gene72286:CDS 30.0%
! TAGGAGTGGGAATTTTAAAT+GGG + chr8.2:21400566-21400585 MS.gene72286:CDS 30.0%
! TTAGGAGTGGGAATTTTAAA+TGG + chr8.2:21400565-21400584 MS.gene72286:CDS 30.0%
!! CAAAAATGATAGTGGAAACA+TGG + chr8.2:21400241-21400260 MS.gene72286:CDS 30.0%
!!! TCTAGTTTTGATATCTCGTT+TGG - chr8.2:21400348-21400367 None:intergenic 30.0%
AATTTGGGAAAAGATGGTCA+TGG + chr8.2:21400699-21400718 MS.gene72286:CDS 35.0%
ATGGAACGCAAAAATGATAG+TGG + chr8.2:21400233-21400252 MS.gene72286:CDS 35.0%
ATGGGAGGATATTATTGTTG+AGG + chr8.2:21400584-21400603 MS.gene72286:CDS 35.0%
CATGTATGTGTGTAAGGATT+TGG - chr8.2:21399994-21400013 None:intergenic 35.0%
CATTGAAGAGATAGATTTCG+TGG + chr8.2:21400446-21400465 MS.gene72286:CDS 35.0%
GAGGATATTATTGTTGAGGA+AGG + chr8.2:21400588-21400607 MS.gene72286:CDS 35.0%
GATATTATTGTTGAGGAAGG+AGG + chr8.2:21400591-21400610 MS.gene72286:CDS 35.0%
GGATTAAGCATGTTGATCAA+CGG + chr8.2:21400742-21400761 MS.gene72286:CDS 35.0%
GGGAATTAAGGATTTGATGA+AGG + chr8.2:21400482-21400501 MS.gene72286:CDS 35.0%
GTGGCTAAGTTAAGTGTTAA+GGG + chr8.2:21400513-21400532 MS.gene72286:CDS 35.0%
TTCATGCATGTATGTGTGTA+AGG - chr8.2:21400000-21400019 None:intergenic 35.0%
!! GGATTTGATGAAGGTTTTGA+AGG + chr8.2:21400491-21400510 MS.gene72286:CDS 35.0%
!!! ATGCTTTTGATGCTGTTTCA+GGG - chr8.2:21399943-21399962 None:intergenic 35.0%
!!! TATGCTTTTGATGCTGTTTC+AGG - chr8.2:21399944-21399963 None:intergenic 35.0%
!!! TTTGATGAAGGTTTTGAAGG+TGG + chr8.2:21400494-21400513 MS.gene72286:CDS 35.0%
ATAGCACATGTTTCAGCTGT+TGG + chr8.2:21400672-21400691 MS.gene72286:CDS 40.0%
GGTAGATTGTGAGGGAATTA+AGG + chr8.2:21400470-21400489 MS.gene72286:CDS 40.0%
GGTGGCTAAGTTAAGTGTTA+AGG + chr8.2:21400512-21400531 MS.gene72286:CDS 40.0%
GGTGTAATCGAAACAAGCTT+AGG + chr8.2:21400273-21400292 MS.gene72286:CDS 40.0%
TAGCACATGTTTCAGCTGTT+GGG + chr8.2:21400673-21400692 MS.gene72286:CDS 40.0%
TGAAGAGATAGATTTCGTGG+TGG + chr8.2:21400449-21400468 MS.gene72286:CDS 40.0%
TTGTTGAGGAAGGAGGATTA+AGG + chr8.2:21400598-21400617 MS.gene72286:CDS 40.0%
! ATAGTGGAAACATGGTCAGA+AGG + chr8.2:21400249-21400268 MS.gene72286:CDS 40.0%
! GAGTGGGAATTTTAAATGGG+AGG + chr8.2:21400569-21400588 MS.gene72286:CDS 40.0%
! GTAAAACCTTGCACTGATGT+AGG - chr8.2:21400170-21400189 None:intergenic 40.0%
!!! TGCTTTTGATGCTGTTTCAG+GGG - chr8.2:21399942-21399961 None:intergenic 40.0%
AAGCATGTTGATCAACGGTC+AGG + chr8.2:21400747-21400766 MS.gene72286:CDS 45.0%
AGCACATGTTTCAGCTGTTG+GGG + chr8.2:21400674-21400693 MS.gene72286:CDS 45.0%
AGCATGTTGATCAACGGTCA+GGG + chr8.2:21400748-21400767 MS.gene72286:CDS 45.0%
GGGGGTAATTTGGGAAAAGA+TGG + chr8.2:21400693-21400712 MS.gene72286:CDS 45.0%
GTGTTTCTTCCTGTTGGGAA+GGG + chr8.2:21400642-21400661 MS.gene72286:CDS 45.0%
TCAGCTGTTGGGGGTAATTT+GGG + chr8.2:21400684-21400703 MS.gene72286:CDS 45.0%
TTCAGCTGTTGGGGGTAATT+TGG + chr8.2:21400683-21400702 MS.gene72286:CDS 45.0%
TTCGTGGTGGTAGATTGTGA+GGG + chr8.2:21400462-21400481 MS.gene72286:CDS 45.0%
TTCTGACCTACATCAGTGCA+AGG + chr8.2:21400161-21400180 MS.gene72286:intron 45.0%
TTTCGTGGTGGTAGATTGTG+AGG + chr8.2:21400461-21400480 MS.gene72286:CDS 45.0%
! CATCAGTGCAAGGTTTTACG+TGG + chr8.2:21400171-21400190 MS.gene72286:intron 45.0%
! GGGAGGTGCATGTTATTAGA+CGG + chr8.2:21400769-21400788 MS.gene72286:CDS 45.0%
! TAAGGTCACGTCGTGTTGTT+AGG + chr8.2:21400616-21400635 MS.gene72286:CDS 45.0%
! TGCAAGGTTTTACGTGGATC+CGG + chr8.2:21400177-21400196 MS.gene72286:CDS 45.0%
AAAGATGGTCATGGTCGTGG+TGG + chr8.2:21400708-21400727 MS.gene72286:CDS 50.0%
ATGTCAAGTCCCTTCCCAAC+AGG - chr8.2:21400654-21400673 None:intergenic 50.0%
GCACATGTTTCAGCTGTTGG+GGG + chr8.2:21400675-21400694 MS.gene72286:CDS 50.0%
GCATGTTGATCAACGGTCAG+GGG + chr8.2:21400749-21400768 MS.gene72286:CDS 50.0%
GGAAAAGATGGTCATGGTCG+TGG + chr8.2:21400705-21400724 MS.gene72286:CDS 50.0%
GGTGTTTCTTCCTGTTGGGA+AGG + chr8.2:21400641-21400660 MS.gene72286:CDS 50.0%
GTCGTGGTGGTAGTAAGAGA+TGG + chr8.2:21400721-21400740 MS.gene72286:CDS 50.0%
GTGGAAACATGGTCAGAAGG+AGG + chr8.2:21400252-21400271 MS.gene72286:CDS 50.0%
! AGGTCGGTGTTTCTTCCTGT+TGG + chr8.2:21400636-21400655 MS.gene72286:CDS 50.0%
! GGTCGGTGTTTCTTCCTGTT+GGG + chr8.2:21400637-21400656 MS.gene72286:CDS 50.0%
! GTCACGTCGTGTTGTTAGGT+CGG + chr8.2:21400620-21400639 MS.gene72286:CDS 50.0%
CTGATTTCAGCCATGGCAGC+AGG + chr8.2:21400210-21400229 MS.gene72286:CDS 55.0%
TGAAATCAGCTCCGCCATGC+CGG - chr8.2:21400199-21400218 None:intergenic 55.0%
TGTTGATCAACGGTCAGGGG+AGG + chr8.2:21400752-21400771 MS.gene72286:CDS 55.0%
TTTACGTGGATCCGGCATGG+CGG + chr8.2:21400185-21400204 MS.gene72286:CDS 55.0%
TTTCAGCCATGGCAGCAGGA+TGG + chr8.2:21400214-21400233 MS.gene72286:CDS 55.0%
! GGTTTTACGTGGATCCGGCA+TGG + chr8.2:21400182-21400201 MS.gene72286:CDS 55.0%
TGCGTTCCATCCTGCTGCCA+TGG - chr8.2:21400223-21400242 None:intergenic 60.0%
! GGCGGAGCTGATTTCAGCCA+TGG + chr8.2:21400203-21400222 MS.gene72286:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 21399923 21400794 21399923 ID=MS.gene72286
chr8.2 mRNA 21399923 21400794 21399923 ID=MS.gene72286.t1;Parent=MS.gene72286
chr8.2 exon 21399923 21399982 21399923 ID=MS.gene72286.t1.exon1;Parent=MS.gene72286.t1
chr8.2 CDS 21399923 21399982 21399923 ID=cds.MS.gene72286.t1;Parent=MS.gene72286.t1
chr8.2 exon 21400177 21400794 21400177 ID=MS.gene72286.t1.exon2;Parent=MS.gene72286.t1
chr8.2 CDS 21400177 21400794 21400177 ID=cds.MS.gene72286.t1;Parent=MS.gene72286.t1
Gene Sequence

>MS.gene72286

ATGAAACTTGTCTGGTCCCCTGAAACAGCATCAAAAGCATACATCGACACAGTTCAATCTGTAAGCAACCAAATCCTTACACACATACATGCATGAATTAATCTTCTTTTTCATTTCCATATTAATCTTGAATAAATAAAATTTTCTCAGCTTAGCGATAAGAGTTTGACTTTACAAAACATAGTCAAAATCTCATCGCATGTTGTAAAAATAACCGTTACTGTTGTTAATAATAATTTTCTGACCTACATCAGTGCAAGGTTTTACGTGGATCCGGCATGGCGGAGCTGATTTCAGCCATGGCAGCAGGATGGAACGCAAAAATGATAGTGGAAACATGGTCAGAAGGAGGTGTAATCGAAACAAGCTTAGGTTTATCAATAGCAAAAAAGCACACAAATGGGAGACACGTATGCATAGTTCCAAACGAGATATCAAAACTAGAGTACTCTAAAAAAATGGGTGAACAAGGAACATCAACAGAAATCATTGTAGGAGAAGCAGAAGAAGTTATGAAAGATTTCATTGAAGAGATAGATTTCGTGGTGGTAGATTGTGAGGGAATTAAGGATTTGATGAAGGTTTTGAAGGTGGCTAAGTTAAGTGTTAAGGGTGCAGTTTTGATATGTAAGAATGTTAATTTTAGGAGTGGGAATTTTAAATGGGAGGATATTATTGTTGAGGAAGGAGGATTAAGGTCACGTCGTGTTGTTAGGTCGGTGTTTCTTCCTGTTGGGAAGGGACTTGACATAGCACATGTTTCAGCTGTTGGGGGTAATTTGGGAAAAGATGGTCATGGTCGTGGTGGTAGTAAGAGATGGATTAAGCATGTTGATCAACGGTCAGGGGAGGTGCATGTTATTAGACGGTGA

Protein sequence

>MS.gene72286.t1

MKLVWSPETASKAYIDTVQSCKVLRGSGMAELISAMAAGWNAKMIVETWSEGGVIETSLGLSIAKKHTNGRHVCIVPNEISKLEYSKKMGEQGTSTEIIVGEAEEVMKDFIEEIDFVVVDCEGIKDLMKVLKVAKLSVKGAVLICKNVNFRSGNFKWEDIIVEEGGLRSRRVVRSVFLPVGKGLDIAHVSAVGGNLGKDGHGRGGSKRWIKHVDQRSGEVHVIRR