AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73462


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73462.t1 MTR_1g108700 91.536 319 27 0 1 319 1 319 5.91e-83 253
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73462.t1 AT2G01100 48.947 190 90 3 1 190 1 183 5.71e-33 122
MS.gene73462.t1 AT2G01100 48.947 190 90 3 1 190 1 183 5.71e-33 122
MS.gene73462.t1 AT2G01100 48.947 190 90 3 1 190 1 183 5.71e-33 122

Find 54 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATCTGATTCTGATTATC+AGG 0.194332 1.4:+83395045 MS.gene73462:CDS
ACTCTGATGATGAGAGTAAA+AGG 0.293068 1.4:+83394528 MS.gene73462:CDS
ACGAAGTCAACAAGAGAAAA+AGG 0.339357 1.4:+83394795 MS.gene73462:CDS
GAAGAAAATATTGGAGAGGA+AGG 0.390822 1.4:+83394373 MS.gene73462:CDS
GAAGCTGGAAGCTGCTGCCA+AGG 0.414532 1.4:+83394418 MS.gene73462:CDS
TTTGAAGTGGGAGCAGAAGC+TGG 0.424827 1.4:+83394403 MS.gene73462:CDS
GTGATCTCCTATGATGCTTA+TGG 0.428176 1.4:-83395006 None:intergenic
CACTAGATTGCGAGTGGCGG+CGG 0.432339 1.4:-83394922 None:intergenic
GAAGGAGAAAGAAGAAGCAA+AGG 0.440557 1.4:+83394714 MS.gene73462:CDS
GGAAGCTGCTGCCAAGGCAA+AGG 0.452183 1.4:+83394424 MS.gene73462:CDS
CCGCTTCTTTGTCTTCCGCT+TGG 0.469488 1.4:-83394633 None:intergenic
TTCCGCATCAGCCTTTGCCT+TGG 0.479719 1.4:-83394435 None:intergenic
GTTCAAGTTTGTTATAATGA+TGG 0.482464 1.4:-83395177 None:intergenic
GAAGACAAAGAAGCGGTCTT+CGG 0.488136 1.4:+83394640 MS.gene73462:CDS
AGCATAAGAATCGGAATCAC+TGG 0.498286 1.4:-83394771 None:intergenic
GACGGCACCATAAGCATCAT+AGG 0.505450 1.4:+83394999 MS.gene73462:CDS
AGCTTCTGCTCCCACTTCAA+AGG 0.506519 1.4:-83394401 None:intergenic
GGGCTAGTAAAAGATCTCAT+AGG 0.508850 1.4:+83394549 MS.gene73462:CDS
TAACAAACTTGAACACCACT+TGG 0.521006 1.4:+83395186 MS.gene73462:CDS
GAGGAGGAATTAGCTAAGAC+TGG 0.524826 1.4:+83395262 MS.gene73462:CDS
GCAGCTCTGAGGAGGAGAGA+AGG 0.526452 1.4:+83394696 MS.gene73462:CDS
GACTTCGTCAGCATAAGAAT+CGG 0.530959 1.4:-83394780 None:intergenic
TGCCAAGGCAAAGGCTGATG+CGG 0.532483 1.4:+83394433 MS.gene73462:CDS
CTCTGATGATGAGAGTAAAA+GGG 0.536453 1.4:+83394529 MS.gene73462:CDS
AACAAGCTGCATGAACCGCT+TGG 0.536708 1.4:-83394339 None:intergenic
CCGATCATCAGAGAAGTCCT+TGG 0.538645 1.4:+83395084 MS.gene73462:CDS
GGAAGGAAGCTCCTTTGAAG+TGG 0.539988 1.4:+83394390 MS.gene73462:CDS
TATAATGATGGCGGCGGTGA+TGG 0.554188 1.4:-83395165 None:intergenic
GATCACGTGATACAGAGGTT+AGG 0.554904 1.4:+83395023 MS.gene73462:CDS
CCAAGGACTTCTCTGATGAT+CGG 0.562981 1.4:-83395084 None:intergenic
TCAGATCGGAAGAGAGGTCA+TGG 0.565374 1.4:+83394884 MS.gene73462:CDS
AAGCATTCTCACATTGACTC+TGG 0.568109 1.4:+83394581 MS.gene73462:CDS
ATGAAAGTGATGATATTCAC+AGG 0.568814 1.4:+83394963 MS.gene73462:CDS
GATTCTGATTATCAGGGTCG+TGG 0.571788 1.4:+83395052 MS.gene73462:CDS
TGTCCAGAGTGTTTATGCAC+TGG 0.574279 1.4:-83395136 None:intergenic
AAAAGAAGAAAATATTGGAG+AGG 0.578009 1.4:+83394369 MS.gene73462:CDS
CAAGTTTGTTATAATGATGG+CGG 0.579411 1.4:-83395174 None:intergenic
ATCTGAAAGCAGCTCTGAGG+AGG 0.584163 1.4:+83394688 MS.gene73462:CDS
GATGCTTATGGTGCCGTCTG+TGG 0.584587 1.4:-83394994 None:intergenic
GTTTGTTATAATGATGGCGG+CGG 0.587426 1.4:-83395171 None:intergenic
AAACCAGTGCATAAACACTC+TGG 0.597676 1.4:+83395133 MS.gene73462:CDS
ACATCTGATTCTGATTATCA+GGG 0.599493 1.4:+83395046 MS.gene73462:CDS
GAAGGAAGCTCCTTTGAAGT+GGG 0.601553 1.4:+83394391 MS.gene73462:CDS
GCGGTTCATGCAGCTTGTTG+AGG 0.609325 1.4:+83394343 MS.gene73462:CDS
AGATTTCTATAGTGACGAAG+AGG 0.614614 1.4:+83394856 MS.gene73462:CDS
TCTGATTATCAGGGTCGTGG+TGG 0.626264 1.4:+83395055 MS.gene73462:CDS
AACAAATGGAAAGCATGAGG+AGG 0.638640 1.4:+83395246 MS.gene73462:CDS
CAGATTCACTAGATTGCGAG+TGG 0.650396 1.4:-83394928 None:intergenic
ACAGGAAGAGAAGCCACAGA+CGG 0.662940 1.4:+83394981 MS.gene73462:CDS
ATTCACTAGATTGCGAGTGG+CGG 0.666042 1.4:-83394925 None:intergenic
GATTTCTCTCGTCAACCAAG+TGG 0.668379 1.4:-83395201 None:intergenic
TAGGAGATCACGTGATACAG+AGG 0.677230 1.4:+83395018 MS.gene73462:CDS
TGAATCTGAAAGCAGCTCTG+AGG 0.683084 1.4:+83394685 MS.gene73462:CDS
CCAAGCGGAAGACAAAGAAG+CGG 0.705691 1.4:+83394633 MS.gene73462:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAAGAAAATATTGGAG+AGG + chr1.4:83394369-83394388 MS.gene73462:CDS 25.0%
! GCAAAACATAAAAAAAGATC+AGG + chr1.4:83394482-83394501 MS.gene73462:CDS 25.0%
! GGAGAAAAAGAAGAAAATAT+TGG + chr1.4:83394364-83394383 MS.gene73462:CDS 25.0%
!! TCTGAATCACTAAATTTTGA+TGG - chr1.4:83394839-83394858 None:intergenic 25.0%
!!! GTTCAAGTTTGTTATAATGA+TGG - chr1.4:83395180-83395199 None:intergenic 25.0%
ACATCTGATTCTGATTATCA+GGG + chr1.4:83395046-83395065 MS.gene73462:CDS 30.0%
ATGAAAGTGATGATATTCAC+AGG + chr1.4:83394963-83394982 MS.gene73462:CDS 30.0%
! AAAAACAAATGGAAAGCATG+AGG + chr1.4:83395243-83395262 MS.gene73462:CDS 30.0%
! TTCTTCAGAATTTTCATCCA+AGG - chr1.4:83395104-83395123 None:intergenic 30.0%
!! CAAGTTTGTTATAATGATGG+CGG - chr1.4:83395177-83395196 None:intergenic 30.0%
!! TCATGCTTTCCATTTGTTTT+TGG - chr1.4:83395244-83395263 None:intergenic 30.0%
AAGTCAACAAGAGAAAAAGG+CGG + chr1.4:83394798-83394817 MS.gene73462:CDS 35.0%
ACGAAGTCAACAAGAGAAAA+AGG + chr1.4:83394795-83394814 MS.gene73462:CDS 35.0%
ACTCTGGTGATCATGAAAAA+AGG + chr1.4:83394597-83394616 MS.gene73462:CDS 35.0%
AGATTTCTATAGTGACGAAG+AGG + chr1.4:83394856-83394875 MS.gene73462:CDS 35.0%
GAAGAAAATATTGGAGAGGA+AGG + chr1.4:83394373-83394392 MS.gene73462:CDS 35.0%
GACATCTGATTCTGATTATC+AGG + chr1.4:83395045-83395064 MS.gene73462:CDS 35.0%
GGAAAGAAAAAAGTTCCAAG+CGG + chr1.4:83394618-83394637 MS.gene73462:CDS 35.0%
TAACAAACTTGAACACCACT+TGG + chr1.4:83395186-83395205 MS.gene73462:CDS 35.0%
! ACGAAGAGGAATTTTCAGAT+CGG + chr1.4:83394870-83394889 MS.gene73462:CDS 35.0%
! ACTCTGATGATGAGAGTAAA+AGG + chr1.4:83394528-83394547 MS.gene73462:CDS 35.0%
! CTCTGATGATGAGAGTAAAA+GGG + chr1.4:83394529-83394548 MS.gene73462:CDS 35.0%
!!! ATTTGTTTTTGGTGAGAGGT+GGG - chr1.4:83395233-83395252 None:intergenic 35.0%
!!! TTCCATTTGTTTTTGGTGAG+AGG - chr1.4:83395237-83395256 None:intergenic 35.0%
AAACCAGTGCATAAACACTC+TGG + chr1.4:83395133-83395152 MS.gene73462:CDS 40.0%
AGCATAAGAATCGGAATCAC+TGG - chr1.4:83394774-83394793 None:intergenic 40.0%
CACCTCTCACCAAAAACAAA+TGG + chr1.4:83395232-83395251 MS.gene73462:CDS 40.0%
GAAGGAGAAAGAAGAAGCAA+AGG + chr1.4:83394714-83394733 MS.gene73462:CDS 40.0%
GACTTCGTCAGCATAAGAAT+CGG - chr1.4:83394783-83394802 None:intergenic 40.0%
GTGATCTCCTATGATGCTTA+TGG - chr1.4:83395009-83395028 None:intergenic 40.0%
! AACAAATGGAAAGCATGAGG+AGG + chr1.4:83395246-83395265 MS.gene73462:CDS 40.0%
! GAATTTTCAGATCGGAAGAG+AGG + chr1.4:83394878-83394897 MS.gene73462:CDS 40.0%
! GGGCTAGTAAAAGATCTCAT+AGG + chr1.4:83394549-83394568 MS.gene73462:CDS 40.0%
!! AAGCATTCTCACATTGACTC+TGG + chr1.4:83394581-83394600 MS.gene73462:CDS 40.0%
!! GTTTGTTATAATGATGGCGG+CGG - chr1.4:83395174-83395193 None:intergenic 40.0%
!!! CATTTGTTTTTGGTGAGAGG+TGG - chr1.4:83395234-83395253 None:intergenic 40.0%
!!! TTTGTTTTTGGTGAGAGGTG+GGG - chr1.4:83395232-83395251 None:intergenic 40.0%
ATTCACTAGATTGCGAGTGG+CGG - chr1.4:83394928-83394947 None:intergenic 45.0%
CAGATTCACTAGATTGCGAG+TGG - chr1.4:83394931-83394950 None:intergenic 45.0%
CCAAGGACTTCTCTGATGAT+CGG - chr1.4:83395087-83395106 None:intergenic 45.0%
GAAGACAAAGAAGCGGTCTT+CGG + chr1.4:83394640-83394659 MS.gene73462:CDS 45.0%
GAGGAGGAATTAGCTAAGAC+TGG + chr1.4:83395262-83395281 MS.gene73462:CDS 45.0%
GATCACGTGATACAGAGGTT+AGG + chr1.4:83395023-83395042 MS.gene73462:CDS 45.0%
GATTCTGATTATCAGGGTCG+TGG + chr1.4:83395052-83395071 MS.gene73462:CDS 45.0%
GATTTCTCTCGTCAACCAAG+TGG - chr1.4:83395204-83395223 None:intergenic 45.0%
TAGGAGATCACGTGATACAG+AGG + chr1.4:83395018-83395037 MS.gene73462:CDS 45.0%
TGAATCTGAAAGCAGCTCTG+AGG + chr1.4:83394685-83394704 MS.gene73462:CDS 45.0%
! TGTCCAGAGTGTTTATGCAC+TGG - chr1.4:83395139-83395158 None:intergenic 45.0%
!! GAAGGAAGCTCCTTTGAAGT+GGG + chr1.4:83394391-83394410 MS.gene73462:CDS 45.0%
AACAAGCTGCATGAACCGCT+TGG - chr1.4:83394342-83394361 None:intergenic 50.0%
ACAAGAGAAAAAGGCGGCAC+AGG + chr1.4:83394804-83394823 MS.gene73462:CDS 50.0%
ACAGGAAGAGAAGCCACAGA+CGG + chr1.4:83394981-83395000 MS.gene73462:CDS 50.0%
AGCTTCTGCTCCCACTTCAA+AGG - chr1.4:83394404-83394423 None:intergenic 50.0%
CCAAGCGGAAGACAAAGAAG+CGG + chr1.4:83394633-83394652 MS.gene73462:CDS 50.0%
CCGATCATCAGAGAAGTCCT+TGG + chr1.4:83395084-83395103 MS.gene73462:CDS 50.0%
GACGGCACCATAAGCATCAT+AGG + chr1.4:83394999-83395018 MS.gene73462:CDS 50.0%
TCAGATCGGAAGAGAGGTCA+TGG + chr1.4:83394884-83394903 MS.gene73462:CDS 50.0%
TCTGATTATCAGGGTCGTGG+TGG + chr1.4:83395055-83395074 MS.gene73462:CDS 50.0%
! GGAAGGAAGCTCCTTTGAAG+TGG + chr1.4:83394390-83394409 MS.gene73462:CDS 50.0%
! TTTGAAGTGGGAGCAGAAGC+TGG + chr1.4:83394403-83394422 MS.gene73462:CDS 50.0%
!! ATCTGAAAGCAGCTCTGAGG+AGG + chr1.4:83394688-83394707 MS.gene73462:CDS 50.0%
!! TATAATGATGGCGGCGGTGA+TGG - chr1.4:83395168-83395187 None:intergenic 50.0%
CCGCTTCTTTGTCTTCCGCT+TGG - chr1.4:83394636-83394655 None:intergenic 55.0%
TGCCAAGGCAAAGGCTGATG+CGG + chr1.4:83394433-83394452 MS.gene73462:CDS 55.0%
TTCCGCATCAGCCTTTGCCT+TGG - chr1.4:83394438-83394457 None:intergenic 55.0%
!! GATGCTTATGGTGCCGTCTG+TGG - chr1.4:83394997-83395016 None:intergenic 55.0%
!! GCGGTTCATGCAGCTTGTTG+AGG + chr1.4:83394343-83394362 MS.gene73462:CDS 55.0%
CACTAGATTGCGAGTGGCGG+CGG - chr1.4:83394925-83394944 None:intergenic 60.0%
GAAGCTGGAAGCTGCTGCCA+AGG + chr1.4:83394418-83394437 MS.gene73462:CDS 60.0%
GGAAGCTGCTGCCAAGGCAA+AGG + chr1.4:83394424-83394443 MS.gene73462:CDS 60.0%
!! GCAGCTCTGAGGAGGAGAGA+AGG + chr1.4:83394696-83394715 MS.gene73462:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 83394332 83395291 83394332 ID=MS.gene73462
chr1.4 mRNA 83394332 83395291 83394332 ID=MS.gene73462.t1;Parent=MS.gene73462
chr1.4 exon 83394332 83395291 83394332 ID=MS.gene73462.t1.exon1;Parent=MS.gene73462.t1
chr1.4 CDS 83394332 83395291 83394332 ID=cds.MS.gene73462.t1;Parent=MS.gene73462.t1
Gene Sequence

>MS.gene73462

ATGGACACCAAGCGGTTCATGCAGCTTGTTGAGGAGAAAAAGAAGAAAATATTGGAGAGGAAGGAAGCTCCTTTGAAGTGGGAGCAGAAGCTGGAAGCTGCTGCCAAGGCAAAGGCTGATGCGGAAGCTAAAGAGAAGAAGCTGAAGACTGCAAAACATAAAAAAAGATCAGGCTCTGATACTGATAGTGATCGTGACTCTGATGATGAGAGTAAAAGGGCTAGTAAAAGATCTCATAGGAAGCACAAGAAGCATTCTCACATTGACTCTGGTGATCATGAAAAAAGGAAAGAAAAAAGTTCCAAGCGGAAGACAAAGAAGCGGTCTTCGGAGTCTAGTGATTTTAGTAGTGATGAATCTGAAAGCAGCTCTGAGGAGGAGAGAAGGAGAAAGAAGAAGCAAAGGAAGAAGATAAGAAATCAAGATTCAAGATCAAATTCCAGTGATTCCGATTCTTATGCTGACGAAGTCAACAAGAGAAAAAGGCGGCACAGGCGTCAGCGTCCATCAAAATTTAGTGATTCAGATTTCTATAGTGACGAAGAGGAATTTTCAGATCGGAAGAGAGGTCATGGAAAGCATCACAAACGCCGCCGCCACTCGCAATCTAGTGAATCTGATTTGTCAAGTGATGAAAGTGATGATATTCACAGGAAGAGAAGCCACAGACGGCACCATAAGCATCATAGGAGATCACGTGATACAGAGGTTAGGACATCTGATTCTGATTATCAGGGTCGTGGTGGAAGAAGCCGATCATCAGAGAAGTCCTTGGATGAAAATTCTGAAGAAGAAAGCAAAAAACCAGTGCATAAACACTCTGGACATCATCACCATCACCGCCGCCATCATTATAACAAACTTGAACACCACTTGGTTGACGAGAGAAATCACTCTCCCCACCTCTCACCAAAAACAAATGGAAAGCATGAGGAGGAATTAGCTAAGACTGGAACTTAA

Protein sequence

>MS.gene73462.t1

MDTKRFMQLVEEKKKKILERKEAPLKWEQKLEAAAKAKADAEAKEKKLKTAKHKKRSGSDTDSDRDSDDESKRASKRSHRKHKKHSHIDSGDHEKRKEKSSKRKTKKRSSESSDFSSDESESSSEEERRRKKKQRKKIRNQDSRSNSSDSDSYADEVNKRKRRHRRQRPSKFSDSDFYSDEEEFSDRKRGHGKHHKRRRHSQSSESDLSSDESDDIHRKRSHRRHHKHHRRSRDTEVRTSDSDYQGRGGRSRSSEKSLDENSEEESKKPVHKHSGHHHHHRRHHYNKLEHHLVDERNHSPHLSPKTNGKHEEELAKTGT