Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75517.t1 | XP_003601907.1 | 94.5 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 7.90E-168 | 599.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75517.t1 | Q8L936 | 49.0 | 345 | 149 | 8 | 6 | 327 | 9 | 349 | 2.2e-73 | 277.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75517.t1 | G7J365 | 94.5 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 5.7e-168 | 599.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene75517 | 0.825543 | 4.43E-54 | -1.69E-46 |
| MS.gene049217 | MS.gene75517 | -0.800514 | 1.39E-48 | -1.69E-46 |
| MS.gene049222 | MS.gene75517 | -0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene049232 | MS.gene75517 | -0.800532 | 1.38E-48 | -1.69E-46 |
| MS.gene049281 | MS.gene75517 | 0.801406 | 9.14E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene75517 | -0.817337 | 3.48E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene75517 | -0.836088 | 1.15E-56 | -1.69E-46 |
| MS.gene049483 | MS.gene75517 | 0.815153 | 1.07E-51 | -1.69E-46 |
| MS.gene049634 | MS.gene75517 | 0.812415 | 4.29E-51 | -1.69E-46 |
| MS.gene04986 | MS.gene75517 | 0.841389 | 4.90E-58 | -1.69E-46 |
| MS.gene05010 | MS.gene75517 | -0.83047 | 2.88E-55 | -1.69E-46 |
| MS.gene050163 | MS.gene75517 | -0.806006 | 1.01E-49 | -1.69E-46 |
| MS.gene050165 | MS.gene75517 | -0.82648 | 2.65E-54 | -1.69E-46 |
| MS.gene050173 | MS.gene75517 | 0.827324 | 1.67E-54 | -1.69E-46 |
| MS.gene050216 | MS.gene75517 | -0.838306 | 3.11E-57 | -1.69E-46 |
| MS.gene050333 | MS.gene75517 | -0.825687 | 4.09E-54 | -1.69E-46 |
| MS.gene050381 | MS.gene75517 | 0.81267 | 3.77E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene75517 | -0.806513 | 7.93E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene75517 | -0.833079 | 6.55E-56 | -1.69E-46 |
| MS.gene050518 | MS.gene75517 | -0.815664 | 8.23E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene75517 | -0.85469 | 1.04E-61 | -1.69E-46 |
| MS.gene050675 | MS.gene75517 | -0.801841 | 7.44E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene75517 | -0.805496 | 1.30E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene75517 | -0.803892 | 2.81E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene75517 | 0.804479 | 2.12E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene75517 | -0.821974 | 3.03E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene75517 | 0.805507 | 1.29E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene75517 | 0.806693 | 7.27E-50 | -1.69E-46 |
| MS.gene051364 | MS.gene75517 | 0.845526 | 3.84E-59 | -1.69E-46 |
| MS.gene051366 | MS.gene75517 | 0.854885 | 9.13E-62 | -1.69E-46 |
| MS.gene051367 | MS.gene75517 | 0.8477 | 9.80E-60 | -1.69E-46 |
| MS.gene051810 | MS.gene75517 | -0.8169 | 4.36E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene75517 | 0.831727 | 1.42E-55 | -1.69E-46 |
| MS.gene053561 | MS.gene75517 | 0.871814 | 5.13E-67 | -1.69E-46 |
| MS.gene054056 | MS.gene75517 | 0.841972 | 3.43E-58 | -1.69E-46 |
| MS.gene054057 | MS.gene75517 | 0.806452 | 8.17E-50 | -1.69E-46 |
| MS.gene054616 | MS.gene75517 | 0.848809 | 4.84E-60 | -1.69E-46 |
| MS.gene054642 | MS.gene75517 | -0.822782 | 1.97E-53 | -1.69E-46 |
| MS.gene054708 | MS.gene75517 | 0.819679 | 1.02E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene75517 | -0.833634 | 4.76E-56 | -1.69E-46 |
| MS.gene055154 | MS.gene75517 | 0.851629 | 7.84E-61 | -1.69E-46 |
| MS.gene055202 | MS.gene75517 | 0.816978 | 4.18E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene75517 | -0.829789 | 4.23E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene75517 | -0.81062 | 1.05E-50 | -1.69E-46 |
| MS.gene055577 | MS.gene75517 | -0.802553 | 5.31E-49 | -1.69E-46 |
| MS.gene055634 | MS.gene75517 | -0.841327 | 5.08E-58 | -1.69E-46 |
| MS.gene055638 | MS.gene75517 | -0.808681 | 2.75E-50 | -1.69E-46 |
| MS.gene055776 | MS.gene75517 | -0.854274 | 1.37E-61 | -1.69E-46 |
| MS.gene055942 | MS.gene75517 | 0.859161 | 5.01E-63 | -1.69E-46 |
| MS.gene056185 | MS.gene75517 | 0.835961 | 1.24E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene75517 | -0.846332 | 2.32E-59 | -1.69E-46 |
| MS.gene056550 | MS.gene75517 | 0.822662 | 2.10E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene75517 | -0.826824 | 2.19E-54 | -1.69E-46 |
| MS.gene056761 | MS.gene75517 | 0.835475 | 1.64E-56 | -1.69E-46 |
| MS.gene057014 | MS.gene75517 | 0.81321 | 2.87E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene75517 | 0.821318 | 4.30E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene75517 | 0.81691 | 4.33E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene75517 | -0.810408 | 1.17E-50 | -1.69E-46 |
| MS.gene058493 | MS.gene75517 | 0.815477 | 9.06E-52 | -1.69E-46 |
| MS.gene058494 | MS.gene75517 | 0.807637 | 4.59E-50 | -1.69E-46 |
| MS.gene058773 | MS.gene75517 | -0.834988 | 2.18E-56 | -1.69E-46 |
| MS.gene05884 | MS.gene75517 | 0.819686 | 1.02E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene75517 | -0.851823 | 6.90E-61 | -1.69E-46 |
| MS.gene059677 | MS.gene75517 | -0.834871 | 2.33E-56 | -1.69E-46 |
| MS.gene059690 | MS.gene75517 | 0.818394 | 2.01E-52 | -1.69E-46 |
| MS.gene059694 | MS.gene75517 | 0.814576 | 1.44E-51 | -1.69E-46 |
| MS.gene059697 | MS.gene75517 | 0.805261 | 1.45E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene75517 | -0.844633 | 6.70E-59 | -1.69E-46 |
| MS.gene060337 | MS.gene75517 | 0.836775 | 7.68E-57 | -1.69E-46 |
| MS.gene060703 | MS.gene75517 | -0.846186 | 2.54E-59 | -1.69E-46 |
| MS.gene060762 | MS.gene75517 | -0.824485 | 7.87E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene75517 | -0.815386 | 9.49E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene75517 | -0.800413 | 1.46E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75517.t1 | MTR_3g086670 | 94.495 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 0.0 | 634 |
| MS.gene75517.t1 | MTR_8g089850 | 62.462 | 325 | 112 | 6 | 7 | 328 | 9 | 326 | 2.38e-131 | 377 |
| MS.gene75517.t1 | MTR_3g099670 | 36.237 | 287 | 172 | 4 | 2 | 279 | 5 | 289 | 1.26e-55 | 184 |
| MS.gene75517.t1 | MTR_4g115420 | 26.875 | 320 | 204 | 12 | 4 | 306 | 9 | 315 | 3.14e-17 | 82.8 |
| MS.gene75517.t1 | MTR_3g106900 | 34.014 | 147 | 92 | 3 | 4 | 147 | 6 | 150 | 2.65e-16 | 80.1 |
| MS.gene75517.t1 | MTR_2g037980 | 29.902 | 204 | 125 | 6 | 4 | 192 | 7 | 207 | 4.05e-16 | 79.3 |
| MS.gene75517.t1 | MTR_4g127440 | 28.571 | 196 | 122 | 5 | 5 | 185 | 8 | 200 | 5.62e-16 | 79.0 |
| MS.gene75517.t1 | MTR_8g008860 | 35.135 | 148 | 90 | 3 | 4 | 147 | 6 | 151 | 9.86e-15 | 75.1 |
| MS.gene75517.t1 | MTR_2g008170 | 33.108 | 148 | 93 | 3 | 4 | 147 | 6 | 151 | 3.06e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75517.t1 | AT4G40080 | 48.286 | 350 | 154 | 8 | 1 | 327 | 4 | 349 | 3.14e-100 | 300 |
| MS.gene75517.t1 | AT5G10410 | 31.875 | 320 | 182 | 9 | 7 | 301 | 8 | 316 | 1.36e-34 | 129 |
| MS.gene75517.t1 | AT5G65370 | 31.046 | 306 | 173 | 9 | 1 | 280 | 1 | 294 | 1.23e-31 | 120 |
| MS.gene75517.t1 | AT5G57200 | 30.049 | 203 | 125 | 6 | 4 | 192 | 7 | 206 | 9.80e-17 | 81.3 |
| MS.gene75517.t1 | AT4G25940 | 28.641 | 206 | 123 | 6 | 1 | 185 | 4 | 206 | 1.05e-16 | 81.3 |
| MS.gene75517.t1 | AT2G01600 | 30.667 | 225 | 137 | 8 | 1 | 210 | 4 | 224 | 8.50e-16 | 78.6 |
| MS.gene75517.t1 | AT4G02650 | 34.000 | 150 | 92 | 3 | 3 | 147 | 5 | 152 | 1.90e-14 | 74.3 |
| MS.gene75517.t1 | AT2G25430 | 31.973 | 147 | 95 | 3 | 4 | 147 | 5 | 149 | 5.23e-14 | 73.2 |
| MS.gene75517.t1 | AT1G14910 | 27.675 | 271 | 166 | 11 | 31 | 284 | 36 | 293 | 6.22e-14 | 73.2 |
| MS.gene75517.t1 | AT5G35200 | 29.947 | 187 | 121 | 6 | 4 | 185 | 11 | 192 | 1.09e-13 | 72.0 |
| MS.gene75517.t1 | AT5G35200 | 29.947 | 187 | 121 | 6 | 4 | 185 | 11 | 192 | 1.09e-13 | 72.0 |
| MS.gene75517.t1 | AT4G32285 | 31.293 | 147 | 96 | 3 | 4 | 147 | 5 | 149 | 1.33e-13 | 72.0 |
| MS.gene75517.t1 | AT4G32285 | 31.293 | 147 | 96 | 3 | 4 | 147 | 5 | 149 | 1.33e-13 | 72.0 |
| MS.gene75517.t1 | AT1G03050 | 32.667 | 150 | 94 | 3 | 3 | 147 | 5 | 152 | 6.72e-13 | 69.7 |
Find 95 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACGGCGATTAAACATTTA+AGG | 0.163434 | 3.3:+72323894 | None:intergenic |
| TTGGTTAGAGTTAAAGAATT+TGG | 0.219230 | 3.3:-72323476 | MS.gene75517:CDS |
| TGGTTAGAGTTAAAGAATTT+GGG | 0.246190 | 3.3:-72323475 | MS.gene75517:CDS |
| GTCAAGAGGTGTTTGTGTTT+TGG | 0.268343 | 3.3:+72324013 | None:intergenic |
| AAACTTGGTTGTCTTGGTTT+TGG | 0.268735 | 3.3:-72323443 | MS.gene75517:CDS |
| TGATTCTTTGTTGGCCTTGA+TGG | 0.273576 | 3.3:-72323606 | MS.gene75517:CDS |
| CAAGGTGATTGTTGAGATAA+TGG | 0.276764 | 3.3:-72323540 | MS.gene75517:CDS |
| GGAGAAGAAATTCTGGGATT+TGG | 0.296571 | 3.3:-72323339 | MS.gene75517:CDS |
| CTGTGTGCTTCTAGAACTCT+TGG | 0.303930 | 3.3:-72323701 | MS.gene75517:CDS |
| TGGTTGAGGAGAAGAAATTC+TGG | 0.306064 | 3.3:-72323346 | MS.gene75517:CDS |
| TATCTTGGGAACTCTCTTCT+TGG | 0.310691 | 3.3:-72323757 | MS.gene75517:CDS |
| AGAACTCTTGGTTTCTTCCT+TGG | 0.315271 | 3.3:-72323689 | MS.gene75517:CDS |
| CTCTTGACTCTTCTTTCCTC+TGG | 0.318672 | 3.3:-72323998 | MS.gene75517:CDS |
| CCATTAGGTTTCCTTCTTCA+AGG | 0.324011 | 3.3:-72323184 | MS.gene75517:CDS |
| TGTTCACTCATTGGAGTATT+AGG | 0.324515 | 3.3:+72323566 | None:intergenic |
| TGGGAGGATGAAGGTGTATA+GGG | 0.331362 | 3.3:-72323294 | MS.gene75517:CDS |
| ACAAAACAAGTTCAATATCT+TGG | 0.345547 | 3.3:-72323772 | MS.gene75517:CDS |
| TTCTCTTAACAGATCACTAT+TGG | 0.348060 | 3.3:+72323631 | None:intergenic |
| TATGGTGATGAATGAAGTTT+TGG | 0.357854 | 3.3:-72323495 | MS.gene75517:CDS |
| ATCTTGGGAACTCTCTTCTT+GGG | 0.358410 | 3.3:-72323756 | MS.gene75517:CDS |
| TTAGGTCTGTTGATTCCATT+AGG | 0.360896 | 3.3:-72323199 | MS.gene75517:CDS |
| ACCTTGTTCTGTTCACTCAT+TGG | 0.361918 | 3.3:+72323557 | None:intergenic |
| GGTGGAAGATGATGGGGTTA+TGG | 0.363211 | 3.3:-72323513 | MS.gene75517:CDS |
| TTGGGAGGATGAAGGTGTAT+AGG | 0.379157 | 3.3:-72323295 | MS.gene75517:CDS |
| GATTGTTGAGATAATGGATT+TGG | 0.380186 | 3.3:-72323534 | MS.gene75517:CDS |
| AAGAGAAACTGATTCTTTGT+TGG | 0.385719 | 3.3:-72323615 | MS.gene75517:CDS |
| AGTTAAAGAATTTGGGGAGA+GGG | 0.391874 | 3.3:-72323468 | MS.gene75517:CDS |
| ATGAAGGTGTATAGGGAAGA+AGG | 0.403447 | 3.3:-72323287 | MS.gene75517:CDS |
| TTGGCCTTGATGGAAGGAAT+TGG | 0.410057 | 3.3:-72323596 | MS.gene75517:CDS |
| AGGGAGAAACTTGGTTGTCT+TGG | 0.426496 | 3.3:-72323449 | MS.gene75517:CDS |
| CTCTGAAGTTTGAGAGGTTG+AGG | 0.434153 | 3.3:+72323795 | None:intergenic |
| GGTTTGATGACAGAGTTCTT+AGG | 0.440749 | 3.3:-72323217 | MS.gene75517:CDS |
| CTGCTGAGTTGTGAGTGGTT+TGG | 0.441669 | 3.3:+72323921 | None:intergenic |
| ACCACCAGTGTAAGGGTAAA+CGG | 0.442204 | 3.3:+72323826 | None:intergenic |
| TGGATTTGGTGGAAGATGAT+GGG | 0.446048 | 3.3:-72323520 | MS.gene75517:CDS |
| CAAAACAAGTTCAATATCTT+GGG | 0.446855 | 3.3:-72323771 | MS.gene75517:CDS |
| TAGTTTCTACCACCAGTGTA+AGG | 0.447417 | 3.3:+72323818 | None:intergenic |
| AACTCTCTTCTTGGGTTCGA+TGG | 0.450996 | 3.3:-72323748 | MS.gene75517:CDS |
| AAGAAACTGAAAGAGGTGAT+AGG | 0.455772 | 3.3:-72324136 | MS.gene75517:CDS |
| AACGGCGATTAAACATTTAA+GGG | 0.462073 | 3.3:+72323895 | None:intergenic |
| CCTTGAAGAAGGAAACCTAA+TGG | 0.462290 | 3.3:+72323184 | None:intergenic |
| AGAGAGAATGATGATGATGA+TGG | 0.463274 | 3.3:-72323372 | MS.gene75517:CDS |
| TTTGGGGAGAGGGAGAAACT+TGG | 0.467834 | 3.3:-72323458 | MS.gene75517:CDS |
| AGAGAATTGAAGGAAAAGGT+TGG | 0.470401 | 3.3:-72323314 | MS.gene75517:CDS |
| GATGATGATGGAGGTGGTTG+AGG | 0.470627 | 3.3:-72323360 | MS.gene75517:CDS |
| CTCTGCGGTGGAGCTTCTCA+TGG | 0.472704 | 3.3:-72323951 | MS.gene75517:CDS |
| GGATTTGGTGAGAGAATTGA+AGG | 0.473257 | 3.3:-72323324 | MS.gene75517:CDS |
| ATGGATTTGGTGGAAGATGA+TGG | 0.474899 | 3.3:-72323521 | MS.gene75517:CDS |
| GAGTTAAAGAATTTGGGGAG+AGG | 0.480788 | 3.3:-72323469 | MS.gene75517:CDS |
| TGGCCTTGATGGAAGGAATT+GGG | 0.482108 | 3.3:-72323595 | MS.gene75517:CDS |
| TGAAGGTGTATAGGGAAGAA+GGG | 0.482642 | 3.3:-72323286 | MS.gene75517:CDS |
| GGTGAGAGAATTGAAGGAAA+AGG | 0.483133 | 3.3:-72323318 | MS.gene75517:CDS |
| AAGCTCCACCGCAGAGGATG+CGG | 0.484245 | 3.3:+72323958 | None:intergenic |
| ACTCTTCTTTCCTCTGGAGA+CGG | 0.488107 | 3.3:-72323992 | MS.gene75517:CDS |
| GAGAATTGAAGGAAAAGGTT+GGG | 0.493649 | 3.3:-72323313 | MS.gene75517:CDS |
| TTTCCCAATTCCTTCCATCA+AGG | 0.501907 | 3.3:+72323592 | None:intergenic |
| GGTTGAGGAGAAGAAATTCT+GGG | 0.518899 | 3.3:-72323345 | MS.gene75517:CDS |
| ATTTACAAGAACCTTGAAGA+AGG | 0.539232 | 3.3:+72323173 | None:intergenic |
| AGAGGTGATAGGATTAATGA+AGG | 0.539540 | 3.3:-72324125 | MS.gene75517:CDS |
| TGTTGAGATAATGGATTTGG+TGG | 0.546687 | 3.3:-72323531 | MS.gene75517:CDS |
| GGTGAAACGACGTCAGAGAA+AGG | 0.547020 | 3.3:-72323668 | MS.gene75517:CDS |
| GTCATCAAACCGATCAGACT+CGG | 0.547520 | 3.3:+72323229 | None:intergenic |
| GGATTTGGTGGAAGATGATG+GGG | 0.552285 | 3.3:-72323519 | MS.gene75517:CDS |
| GGAAAAGGTTGGGAGGATGA+AGG | 0.554877 | 3.3:-72323303 | MS.gene75517:CDS |
| GAGCTGATCACGGAGGATGA+AGG | 0.556396 | 3.3:+72323850 | None:intergenic |
| AGAAGGGAGGCTTAGCAGTA+CGG | 0.556866 | 3.3:-72323270 | MS.gene75517:CDS |
| AGTTGTGAGTGGTTTGGAGA+CGG | 0.568196 | 3.3:+72323927 | None:intergenic |
| TCTTTGTTGGCCTTGATGGA+AGG | 0.570838 | 3.3:-72323602 | MS.gene75517:CDS |
| ACAGAAGAACCGAGTCTGAT+CGG | 0.577525 | 3.3:-72323238 | MS.gene75517:CDS |
| TTCAATGAAGAAACTGAAAG+AGG | 0.579832 | 3.3:-72324143 | None:intergenic |
| TCCAATGAGTGAACAGAACA+AGG | 0.581482 | 3.3:-72323558 | MS.gene75517:CDS |
| TGCCACCGCATCCTCTGCGG+TGG | 0.581913 | 3.3:-72323963 | MS.gene75517:CDS |
| TCCGTTTACCCTTACACTGG+TGG | 0.585451 | 3.3:-72323827 | MS.gene75517:CDS |
| GAGTTCTAGAAGCACACAGA+AGG | 0.587402 | 3.3:+72323705 | None:intergenic |
| CTCCACCGCAGAGGATGCGG+TGG | 0.587882 | 3.3:+72323961 | None:intergenic |
| GGCTACTGCTGAGTTGTGAG+TGG | 0.589218 | 3.3:+72323916 | None:intergenic |
| GGTTAGAGTTAAAGAATTTG+GGG | 0.590495 | 3.3:-72323474 | MS.gene75517:CDS |
| CATGTGTTGTGGCACGTAGG+AGG | 0.596737 | 3.3:+72324050 | None:intergenic |
| AATTGAAGGAAAAGGTTGGG+AGG | 0.600206 | 3.3:-72323310 | MS.gene75517:CDS |
| CTCTCCGTTTACCCTTACAC+TGG | 0.601794 | 3.3:-72323830 | MS.gene75517:CDS |
| TTCTTGGGTTCGATGGTACG+CGG | 0.608953 | 3.3:-72323741 | MS.gene75517:CDS |
| AGTTTCTACCACCAGTGTAA+GGG | 0.622840 | 3.3:+72323819 | None:intergenic |
| AGGTACCGTGTTTGATGATG+TGG | 0.633958 | 3.3:+72323870 | None:intergenic |
| GGCACGTGAACCGTCTCCAG+AGG | 0.642214 | 3.3:+72323982 | None:intergenic |
| AGGTGTATAGGGAAGAAGGG+AGG | 0.646656 | 3.3:-72323283 | MS.gene75517:CDS |
| CATGAGAAGCTCCACCGCAG+AGG | 0.658489 | 3.3:+72323952 | None:intergenic |
| CAGAGGAAAGAAGAGTCAAG+AGG | 0.661118 | 3.3:+72323999 | None:intergenic |
| GTTGAAAGAGTCATGTGTTG+TGG | 0.662998 | 3.3:+72324039 | None:intergenic |
| AGTCATGTGTTGTGGCACGT+AGG | 0.663095 | 3.3:+72324047 | None:intergenic |
| GGTAAACGGAGAGCTGATCA+CGG | 0.664071 | 3.3:+72323840 | None:intergenic |
| GTGTTTGATGATGTGGTGAA+CGG | 0.664912 | 3.3:+72323877 | None:intergenic |
| ACGTGCCACCGCATCCTCTG+CGG | 0.680106 | 3.3:-72323966 | MS.gene75517:CDS |
| TCTCTGACGTCGTTTCACCA+AGG | 0.687518 | 3.3:+72323672 | None:intergenic |
| GTTCACCACATCATCAAACA+CGG | 0.734907 | 3.3:-72323875 | MS.gene75517:CDS |
| AAACGGAGAGCTGATCACGG+AGG | 0.785713 | 3.3:+72323843 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAACAAGTTCAATATCT+TGG | - | chr3.3:72323542-72323561 | MS.gene75517:CDS | 25.0% |
| ! | CAAAACAAGTTCAATATCTT+GGG | - | chr3.3:72323543-72323562 | MS.gene75517:CDS | 25.0% |
| ! | TGGTTAGAGTTAAAGAATTT+GGG | - | chr3.3:72323839-72323858 | MS.gene75517:CDS | 25.0% |
| ! | TTGGTTAGAGTTAAAGAATT+TGG | - | chr3.3:72323838-72323857 | MS.gene75517:CDS | 25.0% |
| AACGGCGATTAAACATTTAA+GGG | + | chr3.3:72323422-72323441 | None:intergenic | 30.0% | |
| GGTTAGAGTTAAAGAATTTG+GGG | - | chr3.3:72323840-72323859 | MS.gene75517:CDS | 30.0% | |
| TTCTCTTAACAGATCACTAT+TGG | + | chr3.3:72323686-72323705 | None:intergenic | 30.0% | |
| ! | AAGAGAAACTGATTCTTTGT+TGG | - | chr3.3:72323699-72323718 | MS.gene75517:CDS | 30.0% |
| !! | GATTGTTGAGATAATGGATT+TGG | - | chr3.3:72323780-72323799 | MS.gene75517:CDS | 30.0% |
| !! | TATGGTGATGAATGAAGTTT+TGG | - | chr3.3:72323819-72323838 | MS.gene75517:CDS | 30.0% |
| AAGAAACTGAAAGAGGTGAT+AGG | - | chr3.3:72323178-72323197 | MS.gene75517:CDS | 35.0% | |
| AGAGAATTGAAGGAAAAGGT+TGG | - | chr3.3:72324000-72324019 | MS.gene75517:CDS | 35.0% | |
| AGAGGTGATAGGATTAATGA+AGG | - | chr3.3:72323189-72323208 | MS.gene75517:CDS | 35.0% | |
| AGTTAAAGAATTTGGGGAGA+GGG | - | chr3.3:72323846-72323865 | MS.gene75517:CDS | 35.0% | |
| CAAGGTGATTGTTGAGATAA+TGG | - | chr3.3:72323774-72323793 | MS.gene75517:CDS | 35.0% | |
| GAACGGCGATTAAACATTTA+AGG | + | chr3.3:72323423-72323442 | None:intergenic | 35.0% | |
| GAGAATTGAAGGAAAAGGTT+GGG | - | chr3.3:72324001-72324020 | MS.gene75517:CDS | 35.0% | |
| ! | AAACTTGGTTGTCTTGGTTT+TGG | - | chr3.3:72323871-72323890 | MS.gene75517:CDS | 35.0% |
| ! | AGAGAGAATGATGATGATGA+TGG | - | chr3.3:72323942-72323961 | MS.gene75517:CDS | 35.0% |
| ! | TGTTCACTCATTGGAGTATT+AGG | + | chr3.3:72323751-72323770 | None:intergenic | 35.0% |
| !! | TGTTGAGATAATGGATTTGG+TGG | - | chr3.3:72323783-72323802 | MS.gene75517:CDS | 35.0% |
| !! | TTAGGTCTGTTGATTCCATT+AGG | - | chr3.3:72324115-72324134 | MS.gene75517:CDS | 35.0% |
| !!! | GGTTTGTGTTTTGAAGAGAT+TGG | - | chr3.3:72323909-72323928 | MS.gene75517:CDS | 35.0% |
| !!! | TTTTGTCTCTGAAGTTTGAG+AGG | + | chr3.3:72323528-72323547 | None:intergenic | 35.0% |
| AATTGAAGGAAAAGGTTGGG+AGG | - | chr3.3:72324004-72324023 | MS.gene75517:CDS | 40.0% | |
| ACCTTGTTCTGTTCACTCAT+TGG | + | chr3.3:72323760-72323779 | None:intergenic | 40.0% | |
| AGAACTCTTGGTTTCTTCCT+TGG | - | chr3.3:72323625-72323644 | MS.gene75517:CDS | 40.0% | |
| AGTTTCTACCACCAGTGTAA+GGG | + | chr3.3:72323498-72323517 | None:intergenic | 40.0% | |
| ATCTTGGGAACTCTCTTCTT+GGG | - | chr3.3:72323558-72323577 | MS.gene75517:CDS | 40.0% | |
| CCATTAGGTTTCCTTCTTCA+AGG | - | chr3.3:72324130-72324149 | MS.gene75517:CDS | 40.0% | |
| CCTTGAAGAAGGAAACCTAA+TGG | + | chr3.3:72324133-72324152 | None:intergenic | 40.0% | |
| GAGTTAAAGAATTTGGGGAG+AGG | - | chr3.3:72323845-72323864 | MS.gene75517:CDS | 40.0% | |
| GGTGAGAGAATTGAAGGAAA+AGG | - | chr3.3:72323996-72324015 | MS.gene75517:CDS | 40.0% | |
| GGTTGAGGAGAAGAAATTCT+GGG | - | chr3.3:72323969-72323988 | MS.gene75517:CDS | 40.0% | |
| GTTCACCACATCATCAAACA+CGG | - | chr3.3:72323439-72323458 | MS.gene75517:CDS | 40.0% | |
| GTTGAAAGAGTCATGTGTTG+TGG | + | chr3.3:72323278-72323297 | None:intergenic | 40.0% | |
| TAGTTTCTACCACCAGTGTA+AGG | + | chr3.3:72323499-72323518 | None:intergenic | 40.0% | |
| TATCTTGGGAACTCTCTTCT+TGG | - | chr3.3:72323557-72323576 | MS.gene75517:CDS | 40.0% | |
| TCCAATGAGTGAACAGAACA+AGG | - | chr3.3:72323756-72323775 | MS.gene75517:CDS | 40.0% | |
| TGGTTGAGGAGAAGAAATTC+TGG | - | chr3.3:72323968-72323987 | MS.gene75517:CDS | 40.0% | |
| TTTCCCAATTCCTTCCATCA+AGG | + | chr3.3:72323725-72323744 | None:intergenic | 40.0% | |
| ! | ATGAAGGTGTATAGGGAAGA+AGG | - | chr3.3:72324027-72324046 | MS.gene75517:CDS | 40.0% |
| ! | GGTTTGATGACAGAGTTCTT+AGG | - | chr3.3:72324097-72324116 | MS.gene75517:CDS | 40.0% |
| ! | TGAAGGTGTATAGGGAAGAA+GGG | - | chr3.3:72324028-72324047 | MS.gene75517:CDS | 40.0% |
| !! | AATGATGATGATGATGGAGG+TGG | - | chr3.3:72323948-72323967 | MS.gene75517:CDS | 40.0% |
| !! | ATGGATTTGGTGGAAGATGA+TGG | - | chr3.3:72323793-72323812 | MS.gene75517:CDS | 40.0% |
| !! | GAGAATGATGATGATGATGG+AGG | - | chr3.3:72323945-72323964 | MS.gene75517:CDS | 40.0% |
| !! | GGAGAAGAAATTCTGGGATT+TGG | - | chr3.3:72323975-72323994 | MS.gene75517:CDS | 40.0% |
| !! | GGATTTGGTGAGAGAATTGA+AGG | - | chr3.3:72323990-72324009 | MS.gene75517:CDS | 40.0% |
| !! | GTGTTTGATGATGTGGTGAA+CGG | + | chr3.3:72323440-72323459 | None:intergenic | 40.0% |
| !! | TGATTCTTTGTTGGCCTTGA+TGG | - | chr3.3:72323708-72323727 | MS.gene75517:CDS | 40.0% |
| !! | TGGATTTGGTGGAAGATGAT+GGG | - | chr3.3:72323794-72323813 | MS.gene75517:CDS | 40.0% |
| !! | TTTTGGAGAAGTGGTGGAAT+TGG | - | chr3.3:72323888-72323907 | MS.gene75517:CDS | 40.0% |
| !!! | AAGAGGTGTTTGTGTTTTGG+AGG | + | chr3.3:72323301-72323320 | None:intergenic | 40.0% |
| !!! | AGAGGTGTTTGTGTTTTGGA+GGG | + | chr3.3:72323300-72323319 | None:intergenic | 40.0% |
| !!! | GTCAAGAGGTGTTTGTGTTT+TGG | + | chr3.3:72323304-72323323 | None:intergenic | 40.0% |
| !!! | TTGTCTTGGTTTTGGAGAAG+TGG | - | chr3.3:72323879-72323898 | MS.gene75517:CDS | 40.0% |
| AACTCTCTTCTTGGGTTCGA+TGG | - | chr3.3:72323566-72323585 | MS.gene75517:CDS | 45.0% | |
| ACAGAAGAACCGAGTCTGAT+CGG | - | chr3.3:72324076-72324095 | MS.gene75517:CDS | 45.0% | |
| ACCACCAGTGTAAGGGTAAA+CGG | + | chr3.3:72323491-72323510 | None:intergenic | 45.0% | |
| AGGGAGAAACTTGGTTGTCT+TGG | - | chr3.3:72323865-72323884 | MS.gene75517:CDS | 45.0% | |
| CAGAGGAAAGAAGAGTCAAG+AGG | + | chr3.3:72323318-72323337 | None:intergenic | 45.0% | |
| CTCTTGACTCTTCTTTCCTC+TGG | - | chr3.3:72323316-72323335 | MS.gene75517:CDS | 45.0% | |
| GTCATCAAACCGATCAGACT+CGG | + | chr3.3:72324088-72324107 | None:intergenic | 45.0% | |
| ! | ACTCTTCTTTCCTCTGGAGA+CGG | - | chr3.3:72323322-72323341 | MS.gene75517:CDS | 45.0% |
| ! | AGTTGTGAGTGGTTTGGAGA+CGG | + | chr3.3:72323390-72323409 | None:intergenic | 45.0% |
| ! | TGGCCTTGATGGAAGGAATT+GGG | - | chr3.3:72323719-72323738 | MS.gene75517:CDS | 45.0% |
| ! | TGGGAGGATGAAGGTGTATA+GGG | - | chr3.3:72324020-72324039 | MS.gene75517:CDS | 45.0% |
| ! | TTGGGAGGATGAAGGTGTAT+AGG | - | chr3.3:72324019-72324038 | MS.gene75517:CDS | 45.0% |
| !! | AGGTACCGTGTTTGATGATG+TGG | + | chr3.3:72323447-72323466 | None:intergenic | 45.0% |
| !! | CTCTGAAGTTTGAGAGGTTG+AGG | + | chr3.3:72323522-72323541 | None:intergenic | 45.0% |
| !! | CTGTGTGCTTCTAGAACTCT+TGG | - | chr3.3:72323613-72323632 | MS.gene75517:CDS | 45.0% |
| !! | GAGTTCTAGAAGCACACAGA+AGG | + | chr3.3:72323612-72323631 | None:intergenic | 45.0% |
| !! | GGATTTGGTGGAAGATGATG+GGG | - | chr3.3:72323795-72323814 | MS.gene75517:CDS | 45.0% |
| !! | TCTTTGTTGGCCTTGATGGA+AGG | - | chr3.3:72323712-72323731 | MS.gene75517:CDS | 45.0% |
| !! | TTGGCCTTGATGGAAGGAAT+TGG | - | chr3.3:72323718-72323737 | MS.gene75517:CDS | 45.0% |
| !!! | TCTTGGTTTTGGAGAAGTGG+TGG | - | chr3.3:72323882-72323901 | MS.gene75517:CDS | 45.0% |
| AGAAGGGAGGCTTAGCAGTA+CGG | - | chr3.3:72324044-72324063 | MS.gene75517:CDS | 50.0% | |
| AGGTGTATAGGGAAGAAGGG+AGG | - | chr3.3:72324031-72324050 | MS.gene75517:CDS | 50.0% | |
| AGTCATGTGTTGTGGCACGT+AGG | + | chr3.3:72323270-72323289 | None:intergenic | 50.0% | |
| CTCTCCGTTTACCCTTACAC+TGG | - | chr3.3:72323484-72323503 | MS.gene75517:CDS | 50.0% | |
| CTGCTGAGTTGTGAGTGGTT+TGG | + | chr3.3:72323396-72323415 | None:intergenic | 50.0% | |
| GGAAAAGGTTGGGAGGATGA+AGG | - | chr3.3:72324011-72324030 | MS.gene75517:CDS | 50.0% | |
| GGTAAACGGAGAGCTGATCA+CGG | + | chr3.3:72323477-72323496 | None:intergenic | 50.0% | |
| GGTGAAACGACGTCAGAGAA+AGG | - | chr3.3:72323646-72323665 | MS.gene75517:CDS | 50.0% | |
| TCCGTTTACCCTTACACTGG+TGG | - | chr3.3:72323487-72323506 | MS.gene75517:CDS | 50.0% | |
| TCTCTGACGTCGTTTCACCA+AGG | + | chr3.3:72323645-72323664 | None:intergenic | 50.0% | |
| TTCTTGGGTTCGATGGTACG+CGG | - | chr3.3:72323573-72323592 | MS.gene75517:CDS | 50.0% | |
| TTTGGGGAGAGGGAGAAACT+TGG | - | chr3.3:72323856-72323875 | MS.gene75517:CDS | 50.0% | |
| !! | GATGATGATGGAGGTGGTTG+AGG | - | chr3.3:72323954-72323973 | MS.gene75517:CDS | 50.0% |
| !! | GGTGGAAGATGATGGGGTTA+TGG | - | chr3.3:72323801-72323820 | MS.gene75517:CDS | 50.0% |
| AAACGGAGAGCTGATCACGG+AGG | + | chr3.3:72323474-72323493 | None:intergenic | 55.0% | |
| CATGTGTTGTGGCACGTAGG+AGG | + | chr3.3:72323267-72323286 | None:intergenic | 55.0% | |
| GAGCTGATCACGGAGGATGA+AGG | + | chr3.3:72323467-72323486 | None:intergenic | 55.0% | |
| GGCTACTGCTGAGTTGTGAG+TGG | + | chr3.3:72323401-72323420 | None:intergenic | 55.0% | |
| AAGCTCCACCGCAGAGGATG+CGG | + | chr3.3:72323359-72323378 | None:intergenic | 60.0% | |
| CATGAGAAGCTCCACCGCAG+AGG | + | chr3.3:72323365-72323384 | None:intergenic | 60.0% | |
| CTCTGCGGTGGAGCTTCTCA+TGG | - | chr3.3:72323363-72323382 | MS.gene75517:CDS | 60.0% | |
| ACGTGCCACCGCATCCTCTG+CGG | - | chr3.3:72323348-72323367 | MS.gene75517:CDS | 65.0% | |
| GGCACGTGAACCGTCTCCAG+AGG | + | chr3.3:72323335-72323354 | None:intergenic | 65.0% | |
| CTCCACCGCAGAGGATGCGG+TGG | + | chr3.3:72323356-72323375 | None:intergenic | 70.0% | |
| TGCCACCGCATCCTCTGCGG+TGG | - | chr3.3:72323351-72323370 | MS.gene75517:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 72323175 | 72324161 | 72323175 | ID=MS.gene75517 |
| chr3.3 | mRNA | 72323175 | 72324161 | 72323175 | ID=MS.gene75517.t1;Parent=MS.gene75517 |
| chr3.3 | exon | 72323175 | 72324161 | 72323175 | ID=MS.gene75517.t1.exon1;Parent=MS.gene75517.t1 |
| chr3.3 | CDS | 72323175 | 72324161 | 72323175 | ID=cds.MS.gene75517.t1;Parent=MS.gene75517.t1 |
| Gene Sequence |
| Protein sequence |
