Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77971.t1 | XP_003590374.1 | 93.8 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 2.90E-53 | 217.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77971.t1 | G7I999 | 93.8 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 2.1e-53 | 217.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene77971 | -0.830972 | 2.17E-55 | -1.69E-46 |
| MS.gene049264 | MS.gene77971 | -0.829486 | 5.01E-55 | -1.69E-46 |
| MS.gene049378 | MS.gene77971 | -0.882891 | 7.12E-71 | -1.69E-46 |
| MS.gene049419 | MS.gene77971 | 0.807157 | 5.80E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene77971 | -0.809003 | 2.35E-50 | -1.69E-46 |
| MS.gene04991 | MS.gene77971 | 0.877716 | 5.03E-69 | -1.69E-46 |
| MS.gene050105 | MS.gene77971 | -0.854157 | 1.48E-61 | -1.69E-46 |
| MS.gene050226 | MS.gene77971 | -0.816419 | 5.59E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene77971 | -0.823307 | 1.49E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene77971 | -0.829543 | 4.85E-55 | -1.69E-46 |
| MS.gene050623 | MS.gene77971 | 0.859027 | 5.49E-63 | -1.69E-46 |
| MS.gene051064 | MS.gene77971 | -0.824909 | 6.25E-54 | -1.69E-46 |
| MS.gene051122 | MS.gene77971 | -0.869469 | 3.03E-66 | -1.69E-46 |
| MS.gene051368 | MS.gene77971 | -0.820015 | 8.57E-53 | -1.69E-46 |
| MS.gene051541 | MS.gene77971 | -0.813511 | 2.47E-51 | -1.69E-46 |
| MS.gene051655 | MS.gene77971 | -0.805454 | 1.32E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene77971 | -0.808931 | 2.43E-50 | -1.69E-46 |
| MS.gene05216 | MS.gene77971 | -0.832515 | 9.05E-56 | -1.69E-46 |
| MS.gene052176 | MS.gene77971 | -0.812486 | 4.14E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene77971 | -0.808837 | 2.55E-50 | -1.69E-46 |
| MS.gene052389 | MS.gene77971 | -0.849926 | 2.36E-60 | -1.69E-46 |
| MS.gene052420 | MS.gene77971 | -0.834661 | 2.63E-56 | -1.69E-46 |
| MS.gene052464 | MS.gene77971 | -0.826857 | 2.16E-54 | -1.69E-46 |
| MS.gene052586 | MS.gene77971 | -0.833623 | 4.79E-56 | -1.69E-46 |
| MS.gene052674 | MS.gene77971 | -0.833734 | 4.50E-56 | -1.69E-46 |
| MS.gene052675 | MS.gene77971 | -0.828282 | 9.80E-55 | -1.69E-46 |
| MS.gene052677 | MS.gene77971 | -0.833405 | 5.43E-56 | -1.69E-46 |
| MS.gene053298 | MS.gene77971 | -0.80163 | 8.22E-49 | -1.69E-46 |
| MS.gene053340 | MS.gene77971 | -0.809512 | 1.82E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene77971 | -0.804852 | 1.77E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene77971 | 0.804874 | 1.75E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene77971 | -0.801971 | 7.00E-49 | -1.69E-46 |
| MS.gene05439 | MS.gene77971 | -0.801943 | 7.09E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene77971 | -0.814167 | 1.77E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene77971 | -0.869671 | 2.60E-66 | -1.69E-46 |
| MS.gene054576 | MS.gene77971 | -0.803463 | 3.44E-49 | -1.69E-46 |
| MS.gene054826 | MS.gene77971 | -0.807469 | 4.98E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene77971 | -0.812724 | 3.67E-51 | -1.69E-46 |
| MS.gene054950 | MS.gene77971 | 0.811273 | 7.60E-51 | -1.69E-46 |
| MS.gene055063 | MS.gene77971 | 0.814733 | 1.33E-51 | -1.69E-46 |
| MS.gene055225 | MS.gene77971 | -0.82791 | 1.20E-54 | -1.69E-46 |
| MS.gene055442 | MS.gene77971 | -0.814818 | 1.27E-51 | -1.69E-46 |
| MS.gene055505 | MS.gene77971 | -0.810731 | 9.96E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene77971 | -0.886605 | 2.96E-72 | -1.69E-46 |
| MS.gene05595 | MS.gene77971 | -0.825754 | 3.94E-54 | -1.69E-46 |
| MS.gene056480 | MS.gene77971 | -0.805377 | 1.37E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene77971 | -0.810914 | 9.09E-51 | -1.69E-46 |
| MS.gene05659 | MS.gene77971 | -0.800418 | 1.45E-48 | -1.69E-46 |
| MS.gene056779 | MS.gene77971 | -0.837858 | 4.06E-57 | -1.69E-46 |
| MS.gene056849 | MS.gene77971 | -0.808651 | 2.79E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene77971 | -0.806734 | 7.12E-50 | -1.69E-46 |
| MS.gene057155 | MS.gene77971 | -0.805149 | 1.53E-49 | -1.69E-46 |
| MS.gene057156 | MS.gene77971 | 0.843932 | 1.03E-58 | -1.69E-46 |
| MS.gene05761 | MS.gene77971 | -0.861414 | 1.04E-63 | -1.69E-46 |
| MS.gene058081 | MS.gene77971 | -0.816459 | 5.47E-52 | -1.69E-46 |
| MS.gene058223 | MS.gene77971 | 0.874461 | 6.63E-68 | -1.69E-46 |
| MS.gene058429 | MS.gene77971 | -0.821359 | 4.21E-53 | -1.69E-46 |
| MS.gene05843 | MS.gene77971 | 0.828889 | 6.99E-55 | -1.69E-46 |
| MS.gene059952 | MS.gene77971 | -0.818424 | 1.97E-52 | -1.69E-46 |
| MS.gene060011 | MS.gene77971 | -0.800873 | 1.17E-48 | -1.69E-46 |
| MS.gene060297 | MS.gene77971 | -0.80959 | 1.75E-50 | -1.69E-46 |
| MS.gene060520 | MS.gene77971 | -0.826331 | 2.88E-54 | -1.69E-46 |
| MS.gene060850 | MS.gene77971 | -0.862901 | 3.65E-64 | -1.69E-46 |
| MS.gene060851 | MS.gene77971 | -0.885175 | 1.02E-71 | -1.69E-46 |
| MS.gene060852 | MS.gene77971 | -0.884625 | 1.63E-71 | -1.69E-46 |
| MS.gene060879 | MS.gene77971 | -0.846344 | 2.30E-59 | -1.69E-46 |
| MS.gene060940 | MS.gene77971 | -0.81865 | 1.75E-52 | -1.69E-46 |
| MS.gene060998 | MS.gene77971 | -0.804679 | 1.92E-49 | -1.69E-46 |
| MS.gene06121 | MS.gene77971 | -0.817948 | 2.53E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77971.t1 | MTR_1g059290 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 4.90e-66 | 195 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77971.t1 | AT2G42310 | 68.932 | 103 | 29 | 1 | 2 | 104 | 14 | 113 | 7.69e-50 | 154 |
| MS.gene77971.t1 | AT3G57785 | 71.000 | 100 | 28 | 1 | 6 | 104 | 14 | 113 | 2.51e-49 | 152 |
Find 44 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCGTCTCCGCGGCGGTTC+AGG | 0.224776 | 1.4:+55198557 | MS.gene77971:CDS |
| TCAAATCAGGCTTAGCATTA+AGG | 0.309891 | 1.4:-55198742 | None:intergenic |
| AGCCCTTGAACGTCTCAAAT+TGG | 0.351342 | 1.4:+55198790 | MS.gene77971:CDS |
| CTGATTTGACTATTGAAACT+TGG | 0.354374 | 1.4:+55198756 | MS.gene77971:CDS |
| TAGGTCTTTCTTCGTGGCCT+GGG | 0.366840 | 1.4:-55198604 | None:intergenic |
| TTAGGTCTTTCTTCGTGGCC+TGG | 0.383907 | 1.4:-55198605 | None:intergenic |
| TGGTTGAAGAGGTAGCCTTT+AGG | 0.396876 | 1.4:-55198623 | None:intergenic |
| CAAGTTTCAATAGTCAAATC+AGG | 0.400055 | 1.4:-55198755 | None:intergenic |
| CTCCGCGGCGGTTCAGGAAC+CGG | 0.401455 | 1.4:+55198563 | MS.gene77971:CDS |
| CACCTGGAGCGAGTGGTGTT+TGG | 0.403944 | 1.4:-55198643 | None:intergenic |
| AATCACGCAAATGGGAAGAT+TGG | 0.415297 | 1.4:+55198666 | MS.gene77971:CDS |
| CTCCAGGTGAATCACGCAAA+TGG | 0.420212 | 1.4:+55198657 | MS.gene77971:CDS |
| CTTCCAATTTGAGACGTTCA+AGG | 0.438923 | 1.4:-55198793 | None:intergenic |
| TCCCATTTGCGTGATTCACC+TGG | 0.448242 | 1.4:-55198659 | None:intergenic |
| ATCACGCAAATGGGAAGATT+GGG | 0.466913 | 1.4:+55198667 | MS.gene77971:CDS |
| CGAGTGGTGTTTGGTTGAAG+AGG | 0.470232 | 1.4:-55198634 | None:intergenic |
| CGTGGCCTGGGCTTGTCCAT+CGG | 0.482648 | 1.4:-55198592 | None:intergenic |
| GCTTGTCCATCGGCTTGCAC+CGG | 0.486213 | 1.4:-55198582 | None:intergenic |
| TCCAGGTGAATCACGCAAAT+GGG | 0.489078 | 1.4:+55198658 | MS.gene77971:CDS |
| TGATTTGACTATTGAAACTT+GGG | 0.489933 | 1.4:+55198757 | MS.gene77971:CDS |
| AACCAAACACCACTCGCTCC+AGG | 0.505049 | 1.4:+55198641 | MS.gene77971:CDS |
| AGCCTTTAGGTCTTTCTTCG+TGG | 0.508189 | 1.4:-55198610 | None:intergenic |
| GCAAGCCGATGGACAAGCCC+AGG | 0.525769 | 1.4:+55198587 | MS.gene77971:CDS |
| GAGGAGACGTTGGCGGAGCA+TGG | 0.529777 | 1.4:-55198528 | None:intergenic |
| TTCCTGAACCGCCGCGGAGA+CGG | 0.531358 | 1.4:-55198559 | None:intergenic |
| TTCCTCACCATCGTTATCCT+CGG | 0.531600 | 1.4:+55198713 | MS.gene77971:CDS |
| TCAAAATCATCTCTATTCAT+CGG | 0.537178 | 1.4:-55198858 | None:intergenic |
| ACCATCGTTATCCTCGGTGT+TGG | 0.550397 | 1.4:+55198719 | MS.gene77971:CDS |
| CAGGAACCGGTGCAAGCCGA+TGG | 0.563436 | 1.4:+55198576 | MS.gene77971:CDS |
| TTCCAATTTGAGACGTTCAA+GGG | 0.567951 | 1.4:-55198792 | None:intergenic |
| GGAGAGAGGTGAGGAGACGT+TGG | 0.578698 | 1.4:-55198538 | None:intergenic |
| GAGAGGTGAGGAGACGTTGG+CGG | 0.579804 | 1.4:-55198535 | None:intergenic |
| GGCCACGAAGAAAGACCTAA+AGG | 0.583150 | 1.4:+55198608 | MS.gene77971:CDS |
| CGCGGAGACGGAGAGAGGTG+AGG | 0.590890 | 1.4:-55198547 | None:intergenic |
| ACCTCTCTCCGTCTCCGCGG+CGG | 0.593678 | 1.4:+55198551 | MS.gene77971:CDS |
| ACCGCCGCGGAGACGGAGAG+AGG | 0.601010 | 1.4:-55198552 | None:intergenic |
| CGTGATTCACCTGGAGCGAG+TGG | 0.616656 | 1.4:-55198650 | None:intergenic |
| AGGAAGCTAGTGATGTAGCA+AGG | 0.627360 | 1.4:-55198695 | None:intergenic |
| CACCGAGGATAACGATGGTG+AGG | 0.629292 | 1.4:-55198715 | None:intergenic |
| CACCGGTTCCTGAACCGCCG+CGG | 0.646325 | 1.4:-55198565 | None:intergenic |
| GGAATCATCAACGACGACAG+CGG | 0.672278 | 1.4:-55198837 | None:intergenic |
| GCCAACACCGAGGATAACGA+TGG | 0.718051 | 1.4:-55198720 | None:intergenic |
| TAGCATTAAGGCCAACACCG+AGG | 0.728768 | 1.4:-55198730 | None:intergenic |
| CTCACCTCTCTCCGTCTCCG+CGG | 0.734494 | 1.4:+55198548 | MS.gene77971:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATTTGACTATTGAAACTT+GGG | + | chr1.4:55198757-55198776 | MS.gene77971:CDS | 25.0% |
| CAAGTTTCAATAGTCAAATC+AGG | - | chr1.4:55198758-55198777 | None:intergenic | 30.0% | |
| ! | CTGATTTGACTATTGAAACT+TGG | + | chr1.4:55198756-55198775 | MS.gene77971:CDS | 30.0% |
| TCAAATCAGGCTTAGCATTA+AGG | - | chr1.4:55198745-55198764 | None:intergenic | 35.0% | |
| ! | TTCCAATTTGAGACGTTCAA+GGG | - | chr1.4:55198795-55198814 | None:intergenic | 35.0% |
| AATCACGCAAATGGGAAGAT+TGG | + | chr1.4:55198666-55198685 | MS.gene77971:CDS | 40.0% | |
| ATCACGCAAATGGGAAGATT+GGG | + | chr1.4:55198667-55198686 | MS.gene77971:CDS | 40.0% | |
| ! | CTTCCAATTTGAGACGTTCA+AGG | - | chr1.4:55198796-55198815 | None:intergenic | 40.0% |
| AGCCCTTGAACGTCTCAAAT+TGG | + | chr1.4:55198790-55198809 | MS.gene77971:CDS | 45.0% | |
| AGCCTTTAGGTCTTTCTTCG+TGG | - | chr1.4:55198613-55198632 | None:intergenic | 45.0% | |
| AGGAAGCTAGTGATGTAGCA+AGG | - | chr1.4:55198698-55198717 | None:intergenic | 45.0% | |
| TCCAGGTGAATCACGCAAAT+GGG | + | chr1.4:55198658-55198677 | MS.gene77971:CDS | 45.0% | |
| TGGTTGAAGAGGTAGCCTTT+AGG | - | chr1.4:55198626-55198645 | None:intergenic | 45.0% | |
| TTCCTCACCATCGTTATCCT+CGG | + | chr1.4:55198713-55198732 | MS.gene77971:CDS | 45.0% | |
| CGAGTGGTGTTTGGTTGAAG+AGG | - | chr1.4:55198637-55198656 | None:intergenic | 50.0% | |
| CTCCAGGTGAATCACGCAAA+TGG | + | chr1.4:55198657-55198676 | MS.gene77971:CDS | 50.0% | |
| GGAATCATCAACGACGACAG+CGG | - | chr1.4:55198840-55198859 | None:intergenic | 50.0% | |
| GGCCACGAAGAAAGACCTAA+AGG | + | chr1.4:55198608-55198627 | MS.gene77971:CDS | 50.0% | |
| TAGCATTAAGGCCAACACCG+AGG | - | chr1.4:55198733-55198752 | None:intergenic | 50.0% | |
| TAGGTCTTTCTTCGTGGCCT+GGG | - | chr1.4:55198607-55198626 | None:intergenic | 50.0% | |
| TCCCATTTGCGTGATTCACC+TGG | - | chr1.4:55198662-55198681 | None:intergenic | 50.0% | |
| TTAGGTCTTTCTTCGTGGCC+TGG | - | chr1.4:55198608-55198627 | None:intergenic | 50.0% | |
| ! | ACCATCGTTATCCTCGGTGT+TGG | + | chr1.4:55198719-55198738 | MS.gene77971:CDS | 50.0% |
| AACCAAACACCACTCGCTCC+AGG | + | chr1.4:55198641-55198660 | MS.gene77971:CDS | 55.0% | |
| CACCGAGGATAACGATGGTG+AGG | - | chr1.4:55198718-55198737 | None:intergenic | 55.0% | |
| GCCAACACCGAGGATAACGA+TGG | - | chr1.4:55198723-55198742 | None:intergenic | 55.0% | |
| CACCTGGAGCGAGTGGTGTT+TGG | - | chr1.4:55198646-55198665 | None:intergenic | 60.0% | |
| CGTGATTCACCTGGAGCGAG+TGG | - | chr1.4:55198653-55198672 | None:intergenic | 60.0% | |
| GAGAGGTGAGGAGACGTTGG+CGG | - | chr1.4:55198538-55198557 | None:intergenic | 60.0% | |
| GGAGAGAGGTGAGGAGACGT+TGG | - | chr1.4:55198541-55198560 | None:intergenic | 60.0% | |
| !! | GCTTGTCCATCGGCTTGCAC+CGG | - | chr1.4:55198585-55198604 | None:intergenic | 60.0% |
| CAGGAACCGGTGCAAGCCGA+TGG | + | chr1.4:55198576-55198595 | MS.gene77971:CDS | 65.0% | |
| CGTGGCCTGGGCTTGTCCAT+CGG | - | chr1.4:55198595-55198614 | None:intergenic | 65.0% | |
| CTCACCTCTCTCCGTCTCCG+CGG | + | chr1.4:55198548-55198567 | MS.gene77971:CDS | 65.0% | |
| GCAAGCCGATGGACAAGCCC+AGG | + | chr1.4:55198587-55198606 | MS.gene77971:CDS | 65.0% | |
| TTCCTGAACCGCCGCGGAGA+CGG | - | chr1.4:55198562-55198581 | None:intergenic | 65.0% | |
| ACCTCTCTCCGTCTCCGCGG+CGG | + | chr1.4:55198551-55198570 | MS.gene77971:CDS | 70.0% | |
| CGCGGAGACGGAGAGAGGTG+AGG | - | chr1.4:55198550-55198569 | None:intergenic | 70.0% | |
| CTCCGCGGCGGTTCAGGAAC+CGG | + | chr1.4:55198563-55198582 | MS.gene77971:CDS | 70.0% | |
| !! | CACCGGTTCCTGAACCGCCG+CGG | - | chr1.4:55198568-55198587 | None:intergenic | 70.0% |
| ACCGCCGCGGAGACGGAGAG+AGG | - | chr1.4:55198555-55198574 | None:intergenic | 75.0% | |
| CTCCGTCTCCGCGGCGGTTC+AGG | + | chr1.4:55198557-55198576 | MS.gene77971:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 55198530 | 55198868 | 55198530 | ID=MS.gene77971 |
| chr1.4 | mRNA | 55198530 | 55198868 | 55198530 | ID=MS.gene77971.t1;Parent=MS.gene77971 |
| chr1.4 | exon | 55198530 | 55198868 | 55198530 | ID=MS.gene77971.t1.exon1;Parent=MS.gene77971.t1 |
| chr1.4 | CDS | 55198530 | 55198868 | 55198530 | ID=cds.MS.gene77971.t1;Parent=MS.gene77971.t1 |
| Gene Sequence |
| Protein sequence |
