Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81086.t1 | XP_013444538.1 | 96.3 | 326 | 12 | 0 | 1 | 326 | 12 | 337 | 3.00E-159 | 571.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81086.t1 | Q700E4 | 46.4 | 323 | 152 | 8 | 10 | 326 | 25 | 332 | 1.7e-54 | 214.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81086.t1 | A0A072TNY8 | 96.3 | 326 | 12 | 0 | 1 | 326 | 12 | 337 | 2.2e-159 | 571.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene81086.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene81086 | 0.804985 | 1.66E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene81086 | 0.810219 | 1.28E-50 | -1.69E-46 |
| MS.gene049389 | MS.gene81086 | 0.810494 | 1.12E-50 | -1.69E-46 |
| MS.gene049491 | MS.gene81086 | 0.865379 | 6.16E-65 | -1.69E-46 |
| MS.gene049661 | MS.gene81086 | 0.821894 | 3.17E-53 | -1.69E-46 |
| MS.gene049662 | MS.gene81086 | 0.816775 | 4.65E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene81086 | 0.882996 | 6.52E-71 | -1.69E-46 |
| MS.gene050076 | MS.gene81086 | 0.859687 | 3.48E-63 | -1.69E-46 |
| MS.gene050099 | MS.gene81086 | 0.84336 | 1.47E-58 | -1.69E-46 |
| MS.gene050128 | MS.gene81086 | 0.84873 | 5.09E-60 | -1.69E-46 |
| MS.gene050301 | MS.gene81086 | 0.872662 | 2.68E-67 | -1.69E-46 |
| MS.gene050397 | MS.gene81086 | 0.84852 | 5.82E-60 | -1.69E-46 |
| MS.gene050404 | MS.gene81086 | 0.805709 | 1.17E-49 | -1.69E-46 |
| MS.gene050406 | MS.gene81086 | 0.810182 | 1.31E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene81086 | 0.834261 | 3.32E-56 | -1.69E-46 |
| MS.gene050529 | MS.gene81086 | 0.827348 | 1.64E-54 | -1.69E-46 |
| MS.gene050767 | MS.gene81086 | 0.818597 | 1.80E-52 | -1.69E-46 |
| MS.gene050770 | MS.gene81086 | 0.821081 | 4.88E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene81086 | 0.856683 | 2.73E-62 | -1.69E-46 |
| MS.gene051471 | MS.gene81086 | 0.857804 | 1.27E-62 | -1.69E-46 |
| MS.gene051662 | MS.gene81086 | 0.817637 | 2.97E-52 | -1.69E-46 |
| MS.gene051663 | MS.gene81086 | 0.829725 | 4.38E-55 | -1.69E-46 |
| MS.gene051860 | MS.gene81086 | 0.828942 | 6.79E-55 | -1.69E-46 |
| MS.gene051877 | MS.gene81086 | 0.814408 | 1.56E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene81086 | 0.888591 | 5.15E-73 | -1.69E-46 |
| MS.gene052178 | MS.gene81086 | 0.874078 | 8.94E-68 | -1.69E-46 |
| MS.gene052188 | MS.gene81086 | 0.815307 | 9.89E-52 | -1.69E-46 |
| MS.gene052287 | MS.gene81086 | 0.847086 | 1.44E-59 | -1.69E-46 |
| MS.gene052290 | MS.gene81086 | 0.80487 | 1.76E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene81086 | 0.846695 | 1.85E-59 | -1.69E-46 |
| MS.gene052326 | MS.gene81086 | 0.861076 | 1.32E-63 | -1.69E-46 |
| MS.gene052340 | MS.gene81086 | 0.843548 | 1.31E-58 | -1.69E-46 |
| MS.gene052485 | MS.gene81086 | 0.823395 | 1.42E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene81086 | 0.803269 | 3.78E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene81086 | 0.829591 | 4.73E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene81086 | 0.868318 | 7.14E-66 | -1.69E-46 |
| MS.gene053836 | MS.gene81086 | 0.871516 | 6.44E-67 | -1.69E-46 |
| MS.gene054123 | MS.gene81086 | 0.816275 | 6.02E-52 | -1.69E-46 |
| MS.gene054214 | MS.gene81086 | 0.804609 | 1.99E-49 | -1.69E-46 |
| MS.gene054376 | MS.gene81086 | 0.82451 | 7.76E-54 | -1.69E-46 |
| MS.gene054473 | MS.gene81086 | 0.818027 | 2.43E-52 | -1.69E-46 |
| MS.gene05455 | MS.gene81086 | 0.834415 | 3.04E-56 | -1.69E-46 |
| MS.gene054802 | MS.gene81086 | 0.816656 | 4.94E-52 | -1.69E-46 |
| MS.gene054803 | MS.gene81086 | 0.817056 | 4.02E-52 | -1.69E-46 |
| MS.gene054804 | MS.gene81086 | 0.814995 | 1.16E-51 | -1.69E-46 |
| MS.gene055116 | MS.gene81086 | 0.808616 | 2.84E-50 | -1.69E-46 |
| MS.gene055254 | MS.gene81086 | 0.828677 | 7.87E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene81086 | 0.889138 | 3.17E-73 | -1.69E-46 |
| MS.gene055390 | MS.gene81086 | 0.851129 | 1.09E-60 | -1.69E-46 |
| MS.gene055422 | MS.gene81086 | 0.889817 | 1.72E-73 | -1.69E-46 |
| MS.gene055428 | MS.gene81086 | 0.875463 | 3.02E-68 | -1.69E-46 |
| MS.gene055604 | MS.gene81086 | 0.807414 | 5.12E-50 | -1.69E-46 |
| MS.gene055712 | MS.gene81086 | 0.812626 | 3.86E-51 | -1.69E-46 |
| MS.gene055896 | MS.gene81086 | 0.8183 | 2.11E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene81086 | 0.841538 | 4.47E-58 | -1.69E-46 |
| MS.gene056314 | MS.gene81086 | 0.837774 | 4.26E-57 | -1.69E-46 |
| MS.gene056407 | MS.gene81086 | 0.808081 | 3.69E-50 | -1.69E-46 |
| MS.gene056410 | MS.gene81086 | 0.835136 | 2.00E-56 | -1.69E-46 |
| MS.gene056609 | MS.gene81086 | 0.821478 | 3.95E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene81086 | 0.842445 | 2.58E-58 | -1.69E-46 |
| MS.gene056796 | MS.gene81086 | 0.805871 | 1.08E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene81086 | 0.859634 | 3.61E-63 | -1.69E-46 |
| MS.gene057121 | MS.gene81086 | 0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene057123 | MS.gene81086 | 0.814141 | 1.79E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene81086 | 0.801685 | 8.01E-49 | -1.69E-46 |
| MS.gene057295 | MS.gene81086 | 0.829033 | 6.45E-55 | -1.69E-46 |
| MS.gene05733 | MS.gene81086 | 0.850168 | 2.02E-60 | -1.69E-46 |
| MS.gene057439 | MS.gene81086 | 0.832005 | 1.21E-55 | -1.69E-46 |
| MS.gene057448 | MS.gene81086 | 0.8166 | 5.09E-52 | -1.69E-46 |
| MS.gene057449 | MS.gene81086 | 0.804429 | 2.17E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene81086 | 0.826396 | 2.78E-54 | -1.69E-46 |
| MS.gene057794 | MS.gene81086 | 0.803732 | 3.03E-49 | -1.69E-46 |
| MS.gene057796 | MS.gene81086 | 0.824428 | 8.12E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene81086 | 0.860279 | 2.31E-63 | -1.69E-46 |
| MS.gene058082 | MS.gene81086 | 0.813538 | 2.43E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene81086 | 0.876723 | 1.11E-68 | -1.69E-46 |
| MS.gene058233 | MS.gene81086 | 0.800358 | 1.50E-48 | -1.69E-46 |
| MS.gene058293 | MS.gene81086 | 0.829821 | 4.15E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene81086 | 0.861971 | 7.05E-64 | -1.69E-46 |
| MS.gene058845 | MS.gene81086 | 0.815873 | 7.40E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene81086 | 0.806123 | 9.58E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene81086 | 0.861705 | 8.50E-64 | -1.69E-46 |
| MS.gene059228 | MS.gene81086 | 0.80913 | 2.20E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene81086 | 0.823263 | 1.52E-53 | -1.69E-46 |
| MS.gene059326 | MS.gene81086 | 0.857023 | 2.16E-62 | -1.69E-46 |
| MS.gene059342 | MS.gene81086 | 0.808602 | 2.86E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene81086 | 0.869519 | 2.91E-66 | -1.69E-46 |
| MS.gene059597 | MS.gene81086 | 0.869299 | 3.44E-66 | -1.69E-46 |
| MS.gene059676 | MS.gene81086 | 0.827589 | 1.44E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene81086 | 0.83405 | 3.75E-56 | -1.69E-46 |
| MS.gene059692 | MS.gene81086 | 0.861037 | 1.36E-63 | -1.69E-46 |
| MS.gene059695 | MS.gene81086 | 0.853235 | 2.73E-61 | -1.69E-46 |
| MS.gene059708 | MS.gene81086 | 0.826241 | 3.02E-54 | -1.69E-46 |
| MS.gene05984 | MS.gene81086 | 0.859788 | 3.24E-63 | -1.69E-46 |
| MS.gene059894 | MS.gene81086 | 0.886393 | 3.56E-72 | -1.69E-46 |
| MS.gene059974 | MS.gene81086 | 0.832308 | 1.02E-55 | -1.69E-46 |
| MS.gene060005 | MS.gene81086 | 0.835591 | 1.53E-56 | -1.69E-46 |
| MS.gene06037 | MS.gene81086 | 0.881193 | 2.94E-70 | -1.69E-46 |
| MS.gene060407 | MS.gene81086 | 0.804057 | 2.59E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene81086 | 0.852944 | 3.31E-61 | -1.69E-46 |
| MS.gene060559 | MS.gene81086 | 0.807101 | 5.96E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene81086 | 0.880224 | 6.54E-70 | -1.69E-46 |
| MS.gene061051 | MS.gene81086 | -0.823943 | 1.05E-53 | -1.69E-46 |
| MS.gene061112 | MS.gene81086 | 0.823257 | 1.53E-53 | -1.69E-46 |
| MS.gene061265 | MS.gene81086 | 0.825419 | 4.74E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81086.t1 | MTR_8g024210 | 98.160 | 326 | 6 | 0 | 1 | 326 | 12 | 337 | 0.0 | 654 |
| MS.gene81086.t1 | MTR_3g099620 | 50.000 | 214 | 89 | 4 | 131 | 326 | 75 | 288 | 1.45e-63 | 204 |
| MS.gene81086.t1 | MTR_0194s0050 | 56.186 | 194 | 67 | 4 | 151 | 326 | 165 | 358 | 4.75e-59 | 194 |
| MS.gene81086.t1 | MTR_7g113830 | 51.309 | 191 | 83 | 2 | 146 | 326 | 92 | 282 | 2.71e-57 | 188 |
| MS.gene81086.t1 | MTR_8g066770 | 51.955 | 179 | 71 | 3 | 160 | 326 | 104 | 279 | 1.36e-53 | 178 |
| MS.gene81086.t1 | MTR_5g064950 | 46.970 | 198 | 83 | 4 | 146 | 326 | 76 | 268 | 1.89e-53 | 177 |
| MS.gene81086.t1 | MTR_5g064950 | 46.970 | 198 | 83 | 4 | 146 | 326 | 76 | 268 | 3.53e-53 | 177 |
| MS.gene81086.t1 | MTR_1g082480 | 51.685 | 178 | 72 | 3 | 163 | 326 | 129 | 306 | 1.32e-51 | 174 |
| MS.gene81086.t1 | MTR_1g082480 | 51.685 | 178 | 72 | 3 | 163 | 326 | 129 | 306 | 1.63e-51 | 173 |
| MS.gene81086.t1 | MTR_2g022280 | 45.455 | 165 | 82 | 1 | 170 | 326 | 1 | 165 | 7.29e-39 | 136 |
| MS.gene81086.t1 | MTR_3g090850 | 39.831 | 236 | 85 | 7 | 147 | 326 | 74 | 308 | 2.04e-38 | 139 |
| MS.gene81086.t1 | MTR_1g028470 | 36.098 | 205 | 102 | 4 | 151 | 326 | 68 | 272 | 1.62e-32 | 122 |
| MS.gene81086.t1 | MTR_1g072320 | 30.986 | 142 | 91 | 3 | 172 | 308 | 471 | 610 | 9.97e-13 | 69.3 |
| MS.gene81086.t1 | MTR_8g027495 | 27.160 | 162 | 95 | 5 | 170 | 326 | 457 | 600 | 3.44e-12 | 67.4 |
| MS.gene81086.t1 | MTR_5g014520 | 28.188 | 149 | 102 | 2 | 172 | 320 | 158 | 301 | 1.70e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81086.t1 | AT2G46810 | 42.818 | 362 | 133 | 12 | 4 | 326 | 22 | 348 | 1.79e-71 | 226 |
| MS.gene81086.t1 | AT2G46810 | 42.897 | 359 | 131 | 12 | 7 | 326 | 13 | 336 | 5.46e-71 | 224 |
| MS.gene81086.t1 | AT3G61950 | 42.815 | 341 | 138 | 8 | 10 | 326 | 25 | 332 | 5.37e-69 | 219 |
| MS.gene81086.t1 | AT3G61950 | 42.815 | 341 | 138 | 8 | 10 | 326 | 25 | 332 | 1.22e-68 | 218 |
| MS.gene81086.t1 | AT2G46810 | 43.408 | 311 | 117 | 9 | 40 | 326 | 1 | 276 | 5.34e-64 | 204 |
| MS.gene81086.t1 | AT2G46810 | 43.408 | 311 | 117 | 9 | 40 | 326 | 1 | 276 | 1.33e-63 | 204 |
| MS.gene81086.t1 | AT3G61950 | 42.765 | 311 | 124 | 7 | 40 | 326 | 1 | 281 | 7.40e-62 | 199 |
| MS.gene81086.t1 | AT3G61950 | 42.765 | 311 | 124 | 7 | 40 | 326 | 1 | 281 | 1.53e-61 | 199 |
| MS.gene81086.t1 | AT4G01460 | 42.718 | 309 | 117 | 8 | 29 | 326 | 20 | 279 | 5.66e-57 | 187 |
| MS.gene81086.t1 | AT4G01460 | 42.718 | 309 | 117 | 8 | 29 | 326 | 20 | 279 | 5.66e-57 | 187 |
| MS.gene81086.t1 | AT3G24140 | 52.792 | 197 | 73 | 2 | 150 | 326 | 177 | 373 | 1.44e-55 | 186 |
| MS.gene81086.t1 | AT3G24140 | 52.792 | 197 | 73 | 2 | 150 | 326 | 177 | 373 | 2.32e-55 | 186 |
| MS.gene81086.t1 | AT1G22490 | 52.247 | 178 | 68 | 3 | 160 | 326 | 105 | 276 | 2.00e-52 | 175 |
| MS.gene81086.t1 | AT1G22490 | 51.685 | 178 | 69 | 3 | 160 | 326 | 105 | 276 | 1.80e-51 | 173 |
| MS.gene81086.t1 | AT1G72210 | 50.568 | 176 | 77 | 3 | 160 | 326 | 115 | 289 | 5.78e-51 | 171 |
| MS.gene81086.t1 | AT1G72210 | 50.568 | 176 | 77 | 3 | 160 | 326 | 115 | 289 | 8.13e-51 | 171 |
| MS.gene81086.t1 | AT3G06120 | 44.186 | 172 | 81 | 3 | 170 | 326 | 1 | 172 | 1.16e-37 | 133 |
| MS.gene81086.t1 | AT5G46690 | 45.455 | 198 | 77 | 4 | 160 | 326 | 78 | 275 | 5.53e-37 | 134 |
| MS.gene81086.t1 | AT5G46690 | 45.455 | 198 | 77 | 4 | 160 | 326 | 78 | 275 | 1.96e-36 | 134 |
| MS.gene81086.t1 | AT3G61950 | 38.967 | 213 | 81 | 5 | 10 | 201 | 25 | 209 | 2.22e-32 | 120 |
| MS.gene81086.t1 | AT3G61950 | 38.967 | 213 | 81 | 5 | 10 | 201 | 25 | 209 | 2.22e-32 | 120 |
| MS.gene81086.t1 | AT5G65320 | 43.023 | 172 | 89 | 3 | 164 | 326 | 96 | 267 | 5.18e-32 | 120 |
| MS.gene81086.t1 | AT5G65320 | 43.023 | 172 | 89 | 3 | 164 | 326 | 96 | 267 | 9.73e-32 | 120 |
| MS.gene81086.t1 | AT5G53210 | 63.889 | 72 | 25 | 1 | 161 | 232 | 94 | 164 | 9.78e-25 | 102 |
| MS.gene81086.t1 | AT5G53210 | 63.889 | 72 | 25 | 1 | 161 | 232 | 94 | 164 | 1.14e-24 | 102 |
| MS.gene81086.t1 | AT1G12860 | 31.677 | 161 | 93 | 5 | 172 | 323 | 268 | 420 | 1.62e-12 | 68.2 |
| MS.gene81086.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 172 | 323 | 308 | 464 | 1.80e-12 | 68.2 |
| MS.gene81086.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 172 | 323 | 308 | 464 | 1.80e-12 | 68.2 |
| MS.gene81086.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 172 | 323 | 308 | 464 | 1.80e-12 | 68.2 |
| MS.gene81086.t1 | AT1G12860 | 31.677 | 161 | 93 | 5 | 172 | 323 | 268 | 420 | 1.88e-12 | 68.2 |
| MS.gene81086.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 172 | 323 | 308 | 464 | 1.95e-12 | 68.2 |
| MS.gene81086.t1 | AT2G16910 | 29.775 | 178 | 96 | 6 | 172 | 326 | 315 | 486 | 5.47e-11 | 63.9 |
| MS.gene81086.t1 | AT1G01260 | 26.178 | 191 | 108 | 7 | 136 | 326 | 404 | 561 | 8.28e-11 | 63.5 |
| MS.gene81086.t1 | AT1G01260 | 26.178 | 191 | 108 | 7 | 136 | 326 | 404 | 561 | 8.28e-11 | 63.5 |
| MS.gene81086.t1 | AT1G01260 | 26.178 | 191 | 108 | 7 | 136 | 326 | 404 | 561 | 8.28e-11 | 63.5 |
Find 39 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGTTGTGAATCCATTCT+AGG | 0.226170 | 8.4:-70850297 | None:intergenic |
| ACATCTTCCTTATTCTTAAC+TGG | 0.278715 | 8.4:-70850540 | None:intergenic |
| AACAAGACCAGTTAAGAATA+AGG | 0.304290 | 8.4:+70850533 | MS.gene81086:CDS |
| TTGTTGCTTTGGAAAATCTT+AGG | 0.306717 | 8.4:+70851359 | MS.gene81086:CDS |
| CTTTGAAGCATTTGAAGAAA+TGG | 0.310096 | 8.4:-70850174 | None:intergenic |
| GATAAAGGTTATTGTTGCTT+TGG | 0.325174 | 8.4:+70851348 | MS.gene81086:CDS |
| TGTTGTTCCTTGTTGAGATT+TGG | 0.330133 | 8.4:-70850487 | None:intergenic |
| GGTGACCAAGCATCAATAAT+TGG | 0.351169 | 8.4:+70851073 | MS.gene81086:CDS |
| ACTCATAAGATGTGATGTTT+AGG | 0.372626 | 8.4:-70851396 | None:intergenic |
| ATGACCACCTTAGTGTTCTT+AGG | 0.380570 | 8.4:+70850619 | MS.gene81086:CDS |
| CACTAAGGTGGTCATTCATT+TGG | 0.381725 | 8.4:-70850611 | None:intergenic |
| TTTGTTCATCAAGTAATGTT+TGG | 0.386937 | 8.4:-70850095 | None:intergenic |
| ATATTGCTGTTGAACGTAAT+AGG | 0.401299 | 8.4:+70850583 | MS.gene81086:CDS |
| CTCCTCCAATTATTGATGCT+TGG | 0.405749 | 8.4:-70851078 | None:intergenic |
| AAAGGCTGGTCAATTGATAA+AGG | 0.417902 | 8.4:+70851333 | MS.gene81086:CDS |
| CATAGCTTACATTCCTAGAA+TGG | 0.419943 | 8.4:+70850284 | MS.gene81086:CDS |
| TCATCATCACCACCTAGTTA+TGG | 0.465171 | 8.4:+70851193 | MS.gene81086:CDS |
| TGCCATCTTCATATGTTCAA+AGG | 0.473511 | 8.4:+70850649 | MS.gene81086:CDS |
| GAATAATACCTTGAGATTGA+AGG | 0.480462 | 8.4:-70851434 | None:intergenic |
| GCCATCTTCATATGTTCAAA+GGG | 0.484046 | 8.4:+70850650 | MS.gene81086:CDS |
| ACAAAGTTTGAGATTTGAAG+AGG | 0.495050 | 8.4:+70850113 | MS.gene81086:CDS |
| TTGTTGCTTTCTGATTTCAC+TGG | 0.503766 | 8.4:-70850381 | None:intergenic |
| TTGTGAAGAAAATGAGGTAA+AGG | 0.510975 | 8.4:+70851231 | MS.gene81086:CDS |
| GATAATTTCATTCCATAACT+AGG | 0.511449 | 8.4:-70851205 | None:intergenic |
| ATGGATTCACAACAAGTTCA+AGG | 0.515635 | 8.4:+70850303 | MS.gene81086:CDS |
| ACCCTTTGAACATATGAAGA+TGG | 0.543479 | 8.4:-70850651 | None:intergenic |
| ATCACCTTGTGAAGAAAATG+AGG | 0.546176 | 8.4:+70851225 | MS.gene81086:CDS |
| CTGTGTAACACAAGAAATGT+TGG | 0.548016 | 8.4:+70850347 | MS.gene81086:CDS |
| ATGTAAGCTATGTCTTCCCA+TGG | 0.586787 | 8.4:-70850273 | None:intergenic |
| ACAAGTTCAAGGCGCAACAT+TGG | 0.592711 | 8.4:+70850314 | MS.gene81086:CDS |
| TAAGAGACCTAAGAACACTA+AGG | 0.612418 | 8.4:-70850626 | None:intergenic |
| ACTCTACTCCTTCAATCTCA+AGG | 0.617899 | 8.4:+70851426 | MS.gene81086:CDS |
| GACCAAGCATCAATAATTGG+AGG | 0.640978 | 8.4:+70851076 | MS.gene81086:CDS |
| AATCTCAACAAGGAACAACA+AGG | 0.648093 | 8.4:+70850490 | MS.gene81086:CDS |
| AGGTGGTGATGATGATGATG+AGG | 0.648803 | 8.4:-70851185 | None:intergenic |
| ATCTCAACAAGGAACAACAA+GGG | 0.668773 | 8.4:+70850491 | MS.gene81086:CDS |
| AATTTCATTCCATAACTAGG+TGG | 0.671528 | 8.4:-70851202 | None:intergenic |
| GAGACCTAAGAACACTAAGG+TGG | 0.679484 | 8.4:-70850623 | None:intergenic |
| TCATGTTCCAAATCTCAACA+AGG | 0.700363 | 8.4:+70850480 | MS.gene81086:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAAAAGAATGAAGATTTT+GGG | + | chr8.4:70851161-70851180 | MS.gene81086:CDS | 15.0% |
| !!! | TTATGTTTTAAAAATCACTT+AGG | + | chr8.4:70850999-70851018 | MS.gene81086:intron | 15.0% |
| !! | TATACAAAACCAAAAAAGAT+TGG | - | chr8.4:70850771-70850790 | None:intergenic | 20.0% |
| !! | TGTTTAATGTTTATGTGATA+GGG | + | chr8.4:70851052-70851071 | MS.gene81086:intron | 20.0% |
| !! | TTGTTTAATGTTTATGTGAT+AGG | + | chr8.4:70851051-70851070 | MS.gene81086:intron | 20.0% |
| !!! | AAAACTTTCAAACACTTTTA+AGG | + | chr8.4:70850232-70850251 | MS.gene81086:CDS | 20.0% |
| !!! | GTAAAAAAGAATGAAGATTT+TGG | + | chr8.4:70851160-70851179 | MS.gene81086:CDS | 20.0% |
| !!! | GTTAAAAATCCAATCTTTTT+TGG | + | chr8.4:70850759-70850778 | MS.gene81086:intron | 20.0% |
| !!! | TTTTTTGGTTTTGTATACAA+GGG | + | chr8.4:70850774-70850793 | MS.gene81086:intron | 20.0% |
| ! | ACATGAAAATCTCAAAAGAA+AGG | - | chr8.4:70850741-70850760 | None:intergenic | 25.0% |
| ! | GAAAATTGAGTGCAAAAAAA+AGG | + | chr8.4:70851315-70851334 | MS.gene81086:CDS | 25.0% |
| ! | GATAATTTCATTCCATAACT+AGG | - | chr8.4:70851208-70851227 | None:intergenic | 25.0% |
| ! | TCAACATATGAAAAAACCAT+GGG | + | chr8.4:70850257-70850276 | MS.gene81086:CDS | 25.0% |
| ! | TTCAACATATGAAAAAACCA+TGG | + | chr8.4:70850256-70850275 | MS.gene81086:CDS | 25.0% |
| ! | TTTGTTCATCAAGTAATGTT+TGG | - | chr8.4:70850098-70850117 | None:intergenic | 25.0% |
| !!! | AAGAAAGGATTTTGAAGAAA+AGG | - | chr8.4:70850726-70850745 | None:intergenic | 25.0% |
| !!! | AATTGATTTTGTGAAAGAGT+TGG | + | chr8.4:70851102-70851121 | MS.gene81086:CDS | 25.0% |
| !!! | AGAAAGGATTTTGAAGAAAA+GGG | - | chr8.4:70850725-70850744 | None:intergenic | 25.0% |
| !!! | CTTTTTTGGTTTTGTATACA+AGG | + | chr8.4:70850773-70850792 | MS.gene81086:intron | 25.0% |
| !!! | TGAAAGTTTTGTTCTTTCAA+AGG | - | chr8.4:70850222-70850241 | None:intergenic | 25.0% |
| AACAAGACCAGTTAAGAATA+AGG | + | chr8.4:70850533-70850552 | MS.gene81086:CDS | 30.0% | |
| AATCTCTTGAAGCACAAAAA+AGG | + | chr8.4:70851137-70851156 | MS.gene81086:CDS | 30.0% | |
| AATTTCATTCCATAACTAGG+TGG | - | chr8.4:70851205-70851224 | None:intergenic | 30.0% | |
| ACATCTTCCTTATTCTTAAC+TGG | - | chr8.4:70850543-70850562 | None:intergenic | 30.0% | |
| ACTCATAAGATGTGATGTTT+AGG | - | chr8.4:70851399-70851418 | None:intergenic | 30.0% | |
| ATCTCTTGAAGCACAAAAAA+GGG | + | chr8.4:70851138-70851157 | MS.gene81086:CDS | 30.0% | |
| TCTTTCAAAGGAAAAAACTG+TGG | - | chr8.4:70850210-70850229 | None:intergenic | 30.0% | |
| TTGTGAAGAAAATGAGGTAA+AGG | + | chr8.4:70851231-70851250 | MS.gene81086:CDS | 30.0% | |
| ! | ACAAAGTTTGAGATTTGAAG+AGG | + | chr8.4:70850113-70850132 | MS.gene81086:CDS | 30.0% |
| ! | ATATTGCTGTTGAACGTAAT+AGG | + | chr8.4:70850583-70850602 | MS.gene81086:CDS | 30.0% |
| ! | GATAAAGGTTATTGTTGCTT+TGG | + | chr8.4:70851348-70851367 | MS.gene81086:CDS | 30.0% |
| ! | TTTACCTCATTTTCTTCACA+AGG | - | chr8.4:70851232-70851251 | None:intergenic | 30.0% |
| !! | CTTTGAAGCATTTGAAGAAA+TGG | - | chr8.4:70850177-70850196 | None:intergenic | 30.0% |
| !! | TTGTTGCTTTGGAAAATCTT+AGG | + | chr8.4:70851359-70851378 | MS.gene81086:CDS | 30.0% |
| !!! | ACTCAATTTTCAAGTTCACA+TGG | - | chr8.4:70851306-70851325 | None:intergenic | 30.0% |
| !!! | CTCAATTTTCAAGTTCACAT+GGG | - | chr8.4:70851305-70851324 | None:intergenic | 30.0% |
| !!! | CTGTTTTTGTTTCTTCTTGT+TGG | - | chr8.4:70850455-70850474 | None:intergenic | 30.0% |
| AATCTCAACAAGGAACAACA+AGG | + | chr8.4:70850490-70850509 | MS.gene81086:CDS | 35.0% | |
| ACCCTTTGAACATATGAAGA+TGG | - | chr8.4:70850654-70850673 | None:intergenic | 35.0% | |
| ATCACCTTGTGAAGAAAATG+AGG | + | chr8.4:70851225-70851244 | MS.gene81086:CDS | 35.0% | |
| ATCTCAACAAGGAACAACAA+GGG | + | chr8.4:70850491-70850510 | MS.gene81086:CDS | 35.0% | |
| ATGGATTCACAACAAGTTCA+AGG | + | chr8.4:70850303-70850322 | MS.gene81086:CDS | 35.0% | |
| ATTGAGTGCAAAAAAAAGGC+TGG | + | chr8.4:70851319-70851338 | MS.gene81086:CDS | 35.0% | |
| CATAGCTTACATTCCTAGAA+TGG | + | chr8.4:70850284-70850303 | MS.gene81086:CDS | 35.0% | |
| CTGTGTAACACAAGAAATGT+TGG | + | chr8.4:70850347-70850366 | MS.gene81086:CDS | 35.0% | |
| GCCATCTTCATATGTTCAAA+GGG | + | chr8.4:70850650-70850669 | MS.gene81086:CDS | 35.0% | |
| TAAGAGACCTAAGAACACTA+AGG | - | chr8.4:70850629-70850648 | None:intergenic | 35.0% | |
| TCATGTTCCAAATCTCAACA+AGG | + | chr8.4:70850480-70850499 | MS.gene81086:CDS | 35.0% | |
| TGCCATCTTCATATGTTCAA+AGG | + | chr8.4:70850649-70850668 | MS.gene81086:CDS | 35.0% | |
| TGTTGTTCCTTGTTGAGATT+TGG | - | chr8.4:70850490-70850509 | None:intergenic | 35.0% | |
| ! | AAAGGCTGGTCAATTGATAA+AGG | + | chr8.4:70851333-70851352 | MS.gene81086:CDS | 35.0% |
| ! | TTTGTATACAAGGGCATGTT+TGG | + | chr8.4:70850783-70850802 | MS.gene81086:intron | 35.0% |
| !! | ACTTGTTGTGAATCCATTCT+AGG | - | chr8.4:70850300-70850319 | None:intergenic | 35.0% |
| !! | TTGTTGCTTTCTGATTTCAC+TGG | - | chr8.4:70850384-70850403 | None:intergenic | 35.0% |
| ATGACCACCTTAGTGTTCTT+AGG | + | chr8.4:70850619-70850638 | MS.gene81086:CDS | 40.0% | |
| ATGTAAGCTATGTCTTCCCA+TGG | - | chr8.4:70850276-70850295 | None:intergenic | 40.0% | |
| CACTAAGGTGGTCATTCATT+TGG | - | chr8.4:70850614-70850633 | None:intergenic | 40.0% | |
| CTCCTCCAATTATTGATGCT+TGG | - | chr8.4:70851081-70851100 | None:intergenic | 40.0% | |
| GACCAAGCATCAATAATTGG+AGG | + | chr8.4:70851076-70851095 | MS.gene81086:CDS | 40.0% | |
| GGTGACCAAGCATCAATAAT+TGG | + | chr8.4:70851073-70851092 | MS.gene81086:CDS | 40.0% | |
| TCATCATCACCACCTAGTTA+TGG | + | chr8.4:70851193-70851212 | MS.gene81086:CDS | 40.0% | |
| ACAAGTTCAAGGCGCAACAT+TGG | + | chr8.4:70850314-70850333 | MS.gene81086:CDS | 45.0% | |
| GAGACCTAAGAACACTAAGG+TGG | - | chr8.4:70850626-70850645 | None:intergenic | 45.0% | |
| ! | AGGTGGTGATGATGATGATG+AGG | - | chr8.4:70851188-70851207 | None:intergenic | 45.0% |
| ! | GGTGGTGATGATGATGATGA+GGG | - | chr8.4:70851187-70851206 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 70850069 | 70851447 | 70850069 | ID=MS.gene81086 |
| chr8.4 | mRNA | 70850069 | 70851447 | 70850069 | ID=MS.gene81086.t1;Parent=MS.gene81086 |
| chr8.4 | exon | 70850069 | 70850671 | 70850069 | ID=MS.gene81086.t1.exon1;Parent=MS.gene81086.t1 |
| chr8.4 | CDS | 70850069 | 70850671 | 70850069 | ID=cds.MS.gene81086.t1;Parent=MS.gene81086.t1 |
| chr8.4 | exon | 70851073 | 70851447 | 70851073 | ID=MS.gene81086.t1.exon2;Parent=MS.gene81086.t1 |
| chr8.4 | CDS | 70851073 | 70851447 | 70851073 | ID=cds.MS.gene81086.t1;Parent=MS.gene81086.t1 |
| Gene Sequence |
| Protein sequence |
