AlfalfaGEDB | MS.gene81388

M. sativa cultivar XinJiangDaYe / MS.gene81388

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81388.t1	XP_003617709.1	94.2	138	8	0	1	138	1	138	3.00E-68	267.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81388.t1	G7K4F2	94.2	138	8	0	1	138	1	138	2.2e-68	267.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049149	MS.gene81388	0.808842	2.54E-50	-1.69E-46
MS.gene049150	MS.gene81388	0.820946	5.24E-53	-1.69E-46
MS.gene049151	MS.gene81388	0.810841	9.43E-51	-1.69E-46
MS.gene049153	MS.gene81388	0.813566	2.40E-51	-1.69E-46
MS.gene049170	MS.gene81388	0.803048	4.20E-49	-1.69E-46
MS.gene049378	MS.gene81388	0.812385	4.35E-51	-1.69E-46
MS.gene049758	MS.gene81388	0.840614	7.82E-58	-1.69E-46
MS.gene049910	MS.gene81388	0.837099	6.35E-57	-1.69E-46
MS.gene049915	MS.gene81388	0.827816	1.27E-54	-1.69E-46
MS.gene050060	MS.gene81388	0.808497	3.01E-50	-1.69E-46
MS.gene050128	MS.gene81388	0.820027	8.52E-53	-1.69E-46
MS.gene050226	MS.gene81388	0.813363	2.66E-51	-1.69E-46
MS.gene050227	MS.gene81388	0.811958	5.40E-51	-1.69E-46
MS.gene050228	MS.gene81388	0.813228	2.85E-51	-1.69E-46
MS.gene050301	MS.gene81388	0.820832	5.57E-53	-1.69E-46
MS.gene050397	MS.gene81388	0.803712	3.06E-49	-1.69E-46
MS.gene050398	MS.gene81388	0.816073	6.67E-52	-1.69E-46
MS.gene050406	MS.gene81388	0.815103	1.10E-51	-1.69E-46
MS.gene050426	MS.gene81388	-0.844925	5.59E-59	-1.69E-46
MS.gene050464	MS.gene81388	0.824605	7.37E-54	-1.69E-46
MS.gene050529	MS.gene81388	0.814941	1.19E-51	-1.69E-46
MS.gene050623	MS.gene81388	-0.825477	4.59E-54	-1.69E-46
MS.gene050642	MS.gene81388	0.843651	1.23E-58	-1.69E-46
MS.gene050670	MS.gene81388	0.809264	2.06E-50	-1.69E-46
MS.gene050971	MS.gene81388	0.809799	1.58E-50	-1.69E-46
MS.gene051535	MS.gene81388	0.809593	1.75E-50	-1.69E-46
MS.gene051860	MS.gene81388	0.830639	2.62E-55	-1.69E-46
MS.gene051983	MS.gene81388	0.865609	5.22E-65	-1.69E-46
MS.gene052077	MS.gene81388	0.900391	7.68E-78	-1.69E-46
MS.gene052178	MS.gene81388	0.817508	3.18E-52	-1.69E-46
MS.gene052188	MS.gene81388	0.857604	1.46E-62	-1.69E-46
MS.gene052253	MS.gene81388	0.818592	1.81E-52	-1.69E-46
MS.gene052310	MS.gene81388	0.810772	9.76E-51	-1.69E-46
MS.gene052340	MS.gene81388	0.866775	2.23E-65	-1.69E-46
MS.gene053073	MS.gene81388	0.829622	4.64E-55	-1.69E-46
MS.gene05316	MS.gene81388	0.83984	1.25E-57	-1.69E-46
MS.gene053494	MS.gene81388	0.837562	4.83E-57	-1.69E-46
MS.gene053557	MS.gene81388	0.838483	2.80E-57	-1.69E-46
MS.gene053577	MS.gene81388	0.80164	8.18E-49	-1.69E-46
MS.gene053836	MS.gene81388	0.846969	1.55E-59	-1.69E-46
MS.gene053928	MS.gene81388	0.843723	1.18E-58	-1.69E-46
MS.gene054183	MS.gene81388	0.805071	1.59E-49	-1.69E-46
MS.gene054349	MS.gene81388	0.851721	7.38E-61	-1.69E-46
MS.gene054542	MS.gene81388	0.820331	7.26E-53	-1.69E-46
MS.gene054556	MS.gene81388	0.804261	2.35E-49	-1.69E-46
MS.gene054930	MS.gene81388	0.850339	1.81E-60	-1.69E-46
MS.gene054983	MS.gene81388	0.802314	5.95E-49	-1.69E-46
MS.gene055116	MS.gene81388	0.826424	2.73E-54	-1.69E-46
MS.gene055422	MS.gene81388	0.817835	2.68E-52	-1.69E-46
MS.gene05553	MS.gene81388	0.843334	1.49E-58	-1.69E-46
MS.gene055701	MS.gene81388	0.807056	6.09E-50	-1.69E-46
MS.gene056447	MS.gene81388	0.813149	2.96E-51	-1.69E-46
MS.gene056659	MS.gene81388	0.818411	1.99E-52	-1.69E-46
MS.gene056979	MS.gene81388	0.855155	7.63E-62	-1.69E-46
MS.gene05702	MS.gene81388	0.825886	3.67E-54	-1.69E-46
MS.gene057396	MS.gene81388	0.860599	1.85E-63	-1.69E-46
MS.gene057448	MS.gene81388	0.813632	2.32E-51	-1.69E-46
MS.gene057449	MS.gene81388	0.803816	2.91E-49	-1.69E-46
MS.gene057450	MS.gene81388	0.815199	1.04E-51	-1.69E-46
MS.gene057754	MS.gene81388	0.822756	2.00E-53	-1.69E-46
MS.gene058002	MS.gene81388	0.833135	6.35E-56	-1.69E-46
MS.gene05811	MS.gene81388	0.835489	1.63E-56	-1.69E-46
MS.gene058142	MS.gene81388	0.847637	1.02E-59	-1.69E-46
MS.gene058276	MS.gene81388	0.820669	6.07E-53	-1.69E-46
MS.gene058710	MS.gene81388	0.823961	1.04E-53	-1.69E-46
MS.gene058845	MS.gene81388	0.800073	1.71E-48	-1.69E-46
MS.gene05907	MS.gene81388	0.840042	1.10E-57	-1.69E-46
MS.gene059247	MS.gene81388	0.802491	5.47E-49	-1.69E-46
MS.gene059285	MS.gene81388	0.855486	6.11E-62	-1.69E-46
MS.gene059294	MS.gene81388	0.810755	9.84E-51	-1.69E-46
MS.gene059420	MS.gene81388	0.807021	6.20E-50	-1.69E-46
MS.gene059597	MS.gene81388	0.804457	2.14E-49	-1.69E-46
MS.gene059688	MS.gene81388	0.800606	1.33E-48	-1.69E-46
MS.gene059692	MS.gene81388	0.809413	1.92E-50	-1.69E-46
MS.gene059695	MS.gene81388	0.805286	1.44E-49	-1.69E-46
MS.gene059788	MS.gene81388	0.839697	1.36E-57	-1.69E-46
MS.gene059807	MS.gene81388	0.812655	3.80E-51	-1.69E-46
MS.gene05984	MS.gene81388	0.852112	5.71E-61	-1.69E-46
MS.gene059894	MS.gene81388	0.834177	3.49E-56	-1.69E-46
MS.gene059899	MS.gene81388	0.801211	1.00E-48	-1.69E-46
MS.gene060084	MS.gene81388	0.807077	6.03E-50	-1.69E-46
MS.gene060403	MS.gene81388	0.801749	7.78E-49	-1.69E-46
MS.gene060495	MS.gene81388	0.845219	4.65E-59	-1.69E-46
MS.gene060940	MS.gene81388	0.815511	8.91E-52	-1.69E-46
MS.gene060942	MS.gene81388	0.818822	1.60E-52	-1.69E-46
MS.gene061002	MS.gene81388	0.825485	4.57E-54	-1.69E-46
MS.gene061049	MS.gene81388	0.858387	8.53E-63	-1.69E-46
MS.gene061154	MS.gene81388	0.817969	2.50E-52	-1.69E-46
MS.gene061159	MS.gene81388	0.851123	1.09E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81388.t1	MTR_5g094600	94.203	138	8	0	1	138	1	138	1.18e-77	226
MS.gene81388.t1	MTR_5g094600	89.899	99	10	0	1	99	1	99	4.18e-43	138

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81388.t1	AT5G55570	53.957	139	62	1	1	137	4	142	6.78e-45	144
MS.gene81388.t1	AT5G55570	51.562	128	60	1	1	126	4	131	1.22e-34	117

Find 18 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTTGTACAATACATTAAC+TGG	0.326787	5.2:+79844976	None:intergenic
TGTTACTATGTTCCTAGCTT+TGG	0.346506	5.2:+79845829	None:intergenic
CAAATTGTGGTTGGAACTAT+AGG	0.347370	5.2:-79845656	MS.gene81388:intron
TCAATAAACATACCACATTC+TGG	0.351241	5.2:+79845222	None:intergenic
TTTCAGGTGGATTTCTTCCT+TGG	0.372047	5.2:-79845021	MS.gene81388:intron
GATTTCTTCCTTGGCAATCC+TGG	0.380191	5.2:-79845012	MS.gene81388:CDS
TCAATCCCTGCCACCACAAA+AGG	0.427439	5.2:+79845428	None:intergenic
TGTGAGATGTGTGGAGGATC+AGG	0.462362	5.2:-79845279	MS.gene81388:CDS
CATGTGATTTCCACAGGAGC+CGG	0.482809	5.2:+79845680	None:intergenic
ATTTGCCATGTGATTTCCAC+AGG	0.496879	5.2:+79845674	None:intergenic
CTTTAACTATTGCCAAAGCT+AGG	0.525649	5.2:-79845841	MS.gene81388:CDS
ATCACATGGCAAATTGTGGT+TGG	0.532719	5.2:-79845665	MS.gene81388:CDS
GCTTTGGCAATAGTTAAAGA+AGG	0.548231	5.2:+79845845	None:intergenic
AGTACTTGATCCGGCTCCTG+TGG	0.548369	5.2:-79845690	MS.gene81388:CDS
TGTAACCACAGTACTTGATC+CGG	0.578314	5.2:-79845699	MS.gene81388:CDS
CGGCTCCTGTGGAAATCACA+TGG	0.593483	5.2:-79845679	MS.gene81388:CDS
GGAAATCACATGGCAAATTG+TGG	0.664563	5.2:-79845669	MS.gene81388:CDS
AGGAGCCGGATCAAGTACTG+TGG	0.738031	5.2:+79845694	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAACACAATACAATACTA+AGG	+	chr5.2:79845361-79845380	None:intergenic	20.0%
!!!	TTTTGGTTCTCAAATATTTA+AGG	-	chr5.2:79845759-79845778	MS.gene81388:CDS	20.0%
!	AACAACAAAAAGAAGAACAA+GGG	-	chr5.2:79845119-79845138	MS.gene81388:intron	25.0%
!	CTTCATATGCTATATATTTC+AGG	-	chr5.2:79845826-79845845	MS.gene81388:CDS	25.0%
!!	ATATGAAGAAATGATTTGTG+AGG	+	chr5.2:79845814-79845833	None:intergenic	25.0%
!!	TTAGGGATAAGAACTATTTT+CGG	-	chr5.2:79845615-79845634	MS.gene81388:intron	25.0%
!!!	ATTGTTTTGAAATTTGTAGC+TGG	-	chr5.2:79845405-79845424	MS.gene81388:intron	25.0%
!!!	CTACTTCTCATTGATTTTTT+TGG	-	chr5.2:79845742-79845761	MS.gene81388:CDS	25.0%
!!!	TTGTTTTGAAATTTGTAGCT+GGG	-	chr5.2:79845406-79845425	MS.gene81388:intron	25.0%
	CAAAAAAAATGTGAGATGTG+TGG	-	chr5.2:79845575-79845594	MS.gene81388:intron	30.0%
	CAACAACAAAAAGAAGAACA+AGG	-	chr5.2:79845118-79845137	MS.gene81388:intron	30.0%
	CATATGCTATATATTTCAGG+TGG	-	chr5.2:79845829-79845848	MS.gene81388:CDS	30.0%
	TCAATAAACATACCACATTC+TGG	+	chr5.2:79845644-79845663	None:intergenic	30.0%
!	TCTAGTTAATTTAGCTCACA+TGG	-	chr5.2:79845280-79845299	MS.gene81388:CDS	30.0%
!	TGAGAAAAAGCTGTGATTTT+GGG	-	chr5.2:79845079-79845098	MS.gene81388:intron	30.0%
	AAAAAATGTGAGATGTGTGG+AGG	-	chr5.2:79845578-79845597	MS.gene81388:intron	35.0%
	ACAAAAAGAAGAACAAGGGT+AGG	-	chr5.2:79845123-79845142	MS.gene81388:intron	35.0%
	CAAATTGTGGTTGGAACTAT+AGG	-	chr5.2:79845207-79845226	MS.gene81388:intron	35.0%
	CTTTAACTATTGCCAAAGCT+AGG	-	chr5.2:79845022-79845041	MS.gene81388:intron	35.0%
	GCTTTGGCAATAGTTAAAGA+AGG	+	chr5.2:79845021-79845040	None:intergenic	35.0%
	TGTTACTATGTTCCTAGCTT+TGG	+	chr5.2:79845037-79845056	None:intergenic	35.0%
!	GAGAAAAAGCTGTGATTTTG+GGG	-	chr5.2:79845080-79845099	MS.gene81388:intron	35.0%
!	GTGAGAAAAAGCTGTGATTT+TGG	-	chr5.2:79845078-79845097	MS.gene81388:intron	35.0%
!!	AAATCACAGCTTTTTCTCAC+TGG	+	chr5.2:79845078-79845097	None:intergenic	35.0%
!!!	AGGATCAGGACTTGTTTTTA+GGG	-	chr5.2:79845598-79845617	MS.gene81388:intron	35.0%
	ATATTCGATATGTCGAGACG+CGG	-	chr5.2:79845319-79845338	MS.gene81388:intron	40.0%
	ATCACATGGCAAATTGTGGT+TGG	-	chr5.2:79845198-79845217	MS.gene81388:intron	40.0%
	ATTTGCCATGTGATTTCCAC+AGG	+	chr5.2:79845192-79845211	None:intergenic	40.0%
	GGAAATCACATGGCAAATTG+TGG	-	chr5.2:79845194-79845213	MS.gene81388:intron	40.0%
	TGTAACCACAGTACTTGATC+CGG	-	chr5.2:79845164-79845183	MS.gene81388:intron	40.0%
!	AATCTTTTCCAGGATTGCCA+AGG	+	chr5.2:79845862-79845881	None:intergenic	40.0%
!	ACTGGAGAAGAATCTTTTCC+AGG	+	chr5.2:79845872-79845891	None:intergenic	40.0%
!	TTTCAGGTGGATTTCTTCCT+TGG	-	chr5.2:79845842-79845861	MS.gene81388:CDS	40.0%
!!	ACAGCTTTTTCTCACTGGTA+AGG	+	chr5.2:79845073-79845092	None:intergenic	40.0%
!!	GAGGATCAGGACTTGTTTTT+AGG	-	chr5.2:79845597-79845616	MS.gene81388:intron	40.0%
!!!	TATTTTCGGTGTCCAGAATG+TGG	-	chr5.2:79845629-79845648	MS.gene81388:intron	40.0%
	GATTTCTTCCTTGGCAATCC+TGG	-	chr5.2:79845851-79845870	MS.gene81388:CDS	45.0%
	TATTCGATATGTCGAGACGC+GGG	-	chr5.2:79845320-79845339	MS.gene81388:intron	45.0%
!	AGCTGGGATTACACCTTTTG+TGG	-	chr5.2:79845422-79845441	MS.gene81388:CDS	45.0%
!	ATTACACCTTTTGTGGTGGC+AGG	-	chr5.2:79845429-79845448	MS.gene81388:CDS	45.0%
!	TGGGATTACACCTTTTGTGG+TGG	-	chr5.2:79845425-79845444	MS.gene81388:CDS	45.0%
!	TTACACCTTTTGTGGTGGCA+GGG	-	chr5.2:79845430-79845449	MS.gene81388:CDS	45.0%
	CATGTGATTTCCACAGGAGC+CGG	+	chr5.2:79845186-79845205	None:intergenic	50.0%
	TCAATCCCTGCCACCACAAA+AGG	+	chr5.2:79845438-79845457	None:intergenic	50.0%
	TGTGAGATGTGTGGAGGATC+AGG	-	chr5.2:79845584-79845603	MS.gene81388:intron	50.0%
	AGTACTTGATCCGGCTCCTG+TGG	-	chr5.2:79845173-79845192	MS.gene81388:intron	55.0%
	CGGCTCCTGTGGAAATCACA+TGG	-	chr5.2:79845184-79845203	MS.gene81388:intron	55.0%
!	AGGAGCCGGATCAAGTACTG+TGG	+	chr5.2:79845172-79845191	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	79844991	79845894	79844991	ID=MS.gene81388
chr5.2	mRNA	79844991	79845894	79844991	ID=MS.gene81388.t1;Parent=MS.gene81388
chr5.2	exon	79845657	79845894	79845657	ID=MS.gene81388.t1.exon1;Parent=MS.gene81388.t1
chr5.2	CDS	79845657	79845894	79845657	ID=cds.MS.gene81388.t1;Parent=MS.gene81388.t1
chr5.2	exon	79845412	79845461	79845412	ID=MS.gene81388.t1.exon2;Parent=MS.gene81388.t1
chr5.2	CDS	79845412	79845461	79845412	ID=cds.MS.gene81388.t1;Parent=MS.gene81388.t1
chr5.2	exon	79845235	79845316	79845235	ID=MS.gene81388.t1.exon3;Parent=MS.gene81388.t1
chr5.2	CDS	79845235	79845316	79845235	ID=cds.MS.gene81388.t1;Parent=MS.gene81388.t1
chr5.2	exon	79844991	79845037	79844991	ID=MS.gene81388.t1.exon4;Parent=MS.gene81388.t1
chr5.2	CDS	79844991	79845037	79844991	ID=cds.MS.gene81388.t1;Parent=MS.gene81388.t1

Gene Sequence

>MS.gene81388

ATGGAAGCTTTAGTTTGTTGTTCTCTGCCTTCTTTAACTATTGCCAAAGCTAGGAACATAGTAACACATAATTGTTGTTCCTTACCAGTGAGAAAAAGCTGTGATTTTGGGGTTAAGAATGCAAGAACAACAACAAAAAGAAGAACAAGGGTAGGTGTTGTGTCTGTGAGTGATGTAACCACAGTACTTGATCCGGCTCCTGTGGAAATCACATGGCAAATTGTGGTTGGAACTATAGGTAATATCTTGTTTACTTTAATGTTAATCTATTTAGAAAGAGAATGACTAGTCTAGTTAATTTAGCTCACATGGTAAAAAAATTGTATAGATATTCGATATGTCGAGACGCGGGTTCTAACTCGTAAGTCCTTAGTATTGTATTGTGTTATTTGAGTTTTTGATTTTGAGTGTTTTATTGTTTTGAAATTTGTAGCTGGGATTACACCTTTTGTGGTGGCAGGGATTGAATTTAGCAAGAGAATTGTAAGTCACTTTATTTGCTTGATTATTGCTTTCTCATAAATTATGAATCTCATTCTTGTGTGTTTTTTGTCATCAATTTTGAATATTTGTTACAGATTGCTCAAAAAAAATGTGAGATGTGTGGAGGATCAGGACTTGTTTTTAGGGATAAGAACTATTTTCGGTGTCCAGAATGTGGTATGTTTATTGAATTCTCTTAATCTTGATTATAAGCATTATTATCATCATTTTGATCATGCTTTAGTTTAAAATGACAATTAGAAACTTGCTACTTCTCATTGATTTTTTTGGTTCTCAAATATTTAAGGTAAGTTAATTACTTGTGTGAAATCGATTGCCTCACAAATCATTTCTTCATATGCTATATATTTCAGGTGGATTTCTTCCTTGGCAATCCTGGAAAAGATTCTTCTCCAGTTAA

Protein sequence

>MS.gene81388.t1

MEALVCCSLPSLTIAKARNIVTHNCCSLPVRKSCDFGVKNARTTTKRRTRVGVVSVSDVTTVLDPAPVEITWQIVVGTIAGITPFVVAGIEFSKRIIAQKKCEMCGGSGLVFRDKNYFRCPECGGFLPWQSWKRFFSS