AlfalfaGEDB | MS.gene86350

M. sativa cultivar XinJiangDaYe / MS.gene86350

KEGG: K02948
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00411.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86350.t1	XP_003597842.2	98	151	3	0	1	151	88	238	1.30E-77	298.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86350.t1	Q8VZT8	58.6	152	62	1	1	151	163	314	1.0e-46	187.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86350.t1	G7IMH8	98.0	151	3	0	1	151	88	238	9.6e-78	298.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene86350	0.814125	1.81E-51	-1.69E-46
MS.gene049341	MS.gene86350	0.808804	2.59E-50	-1.69E-46
MS.gene049382	MS.gene86350	0.80349	3.40E-49	-1.69E-46
MS.gene049756	MS.gene86350	0.811975	5.35E-51	-1.69E-46
MS.gene050277	MS.gene86350	0.895836	6.56E-76	-1.69E-46
MS.gene050303	MS.gene86350	0.812613	3.88E-51	-1.69E-46
MS.gene050719	MS.gene86350	0.815626	8.39E-52	-1.69E-46
MS.gene050721	MS.gene86350	0.820644	6.15E-53	-1.69E-46
MS.gene050724	MS.gene86350	0.803	4.30E-49	-1.69E-46
MS.gene050807	MS.gene86350	0.800093	1.69E-48	-1.69E-46
MS.gene050815	MS.gene86350	0.826793	2.23E-54	-1.69E-46
MS.gene050838	MS.gene86350	0.803643	3.16E-49	-1.69E-46
MS.gene051063	MS.gene86350	0.841722	4.00E-58	-1.69E-46
MS.gene051243	MS.gene86350	0.821699	3.51E-53	-1.69E-46
MS.gene051245	MS.gene86350	0.831318	1.79E-55	-1.69E-46
MS.gene051328	MS.gene86350	0.818088	2.35E-52	-1.69E-46
MS.gene051349	MS.gene86350	0.813821	2.11E-51	-1.69E-46
MS.gene05134	MS.gene86350	0.81514	1.08E-51	-1.69E-46
MS.gene051451	MS.gene86350	0.802676	5.01E-49	-1.69E-46
MS.gene051633	MS.gene86350	0.847579	1.06E-59	-1.69E-46
MS.gene051746	MS.gene86350	0.856257	3.63E-62	-1.69E-46
MS.gene051748	MS.gene86350	0.846517	2.07E-59	-1.69E-46
MS.gene051757	MS.gene86350	0.81792	2.57E-52	-1.69E-46
MS.gene051850	MS.gene86350	0.806526	7.88E-50	-1.69E-46
MS.gene051903	MS.gene86350	0.836877	7.23E-57	-1.69E-46
MS.gene052256	MS.gene86350	0.811188	7.93E-51	-1.69E-46
MS.gene052286	MS.gene86350	0.840778	7.08E-58	-1.69E-46
MS.gene052302	MS.gene86350	0.846151	2.60E-59	-1.69E-46
MS.gene052309	MS.gene86350	-0.803591	3.24E-49	-1.69E-46
MS.gene052376	MS.gene86350	0.843522	1.33E-58	-1.69E-46
MS.gene052665	MS.gene86350	0.800912	1.15E-48	-1.69E-46
MS.gene052856	MS.gene86350	0.840619	7.80E-58	-1.69E-46
MS.gene052858	MS.gene86350	0.810697	1.01E-50	-1.69E-46
MS.gene052860	MS.gene86350	0.814494	1.50E-51	-1.69E-46
MS.gene052947	MS.gene86350	0.842064	3.25E-58	-1.69E-46
MS.gene053109	MS.gene86350	0.810114	1.35E-50	-1.69E-46
MS.gene053132	MS.gene86350	0.803078	4.14E-49	-1.69E-46
MS.gene053216	MS.gene86350	0.820912	5.34E-53	-1.69E-46
MS.gene053437	MS.gene86350	0.815857	7.46E-52	-1.69E-46
MS.gene055162	MS.gene86350	0.812812	3.51E-51	-1.69E-46
MS.gene055174	MS.gene86350	0.8326	8.62E-56	-1.69E-46
MS.gene055424	MS.gene86350	0.821995	3.00E-53	-1.69E-46
MS.gene055441	MS.gene86350	0.824602	7.38E-54	-1.69E-46
MS.gene055512	MS.gene86350	0.802056	6.72E-49	-1.69E-46
MS.gene055808	MS.gene86350	0.803562	3.29E-49	-1.69E-46
MS.gene056069	MS.gene86350	-0.834028	3.80E-56	-1.69E-46
MS.gene056091	MS.gene86350	0.801568	8.47E-49	-1.69E-46
MS.gene056432	MS.gene86350	0.828031	1.13E-54	-1.69E-46
MS.gene056580	MS.gene86350	0.81862	1.78E-52	-1.69E-46
MS.gene056601	MS.gene86350	0.84792	8.52E-60	-1.69E-46
MS.gene056631	MS.gene86350	0.854097	1.54E-61	-1.69E-46
MS.gene056760	MS.gene86350	0.831473	1.64E-55	-1.69E-46
MS.gene057057	MS.gene86350	0.860064	2.68E-63	-1.69E-46
MS.gene057079	MS.gene86350	0.820876	5.44E-53	-1.69E-46
MS.gene057094	MS.gene86350	0.820999	5.10E-53	-1.69E-46
MS.gene057140	MS.gene86350	0.844119	9.21E-59	-1.69E-46
MS.gene057234	MS.gene86350	0.876819	1.03E-68	-1.69E-46
MS.gene057260	MS.gene86350	0.81729	3.56E-52	-1.69E-46
MS.gene057705	MS.gene86350	0.801104	1.05E-48	-1.69E-46
MS.gene05795	MS.gene86350	0.837619	4.67E-57	-1.69E-46
MS.gene058054	MS.gene86350	0.807263	5.50E-50	-1.69E-46
MS.gene058055	MS.gene86350	0.859476	4.03E-63	-1.69E-46
MS.gene058084	MS.gene86350	0.841864	3.67E-58	-1.69E-46
MS.gene058182	MS.gene86350	0.844609	6.80E-59	-1.69E-46
MS.gene058238	MS.gene86350	0.834447	2.98E-56	-1.69E-46
MS.gene058798	MS.gene86350	0.812987	3.21E-51	-1.69E-46
MS.gene058906	MS.gene86350	0.883566	4.02E-71	-1.69E-46
MS.gene059898	MS.gene86350	0.850996	1.18E-60	-1.69E-46
MS.gene06001	MS.gene86350	0.874601	5.94E-68	-1.69E-46
MS.gene060165	MS.gene86350	0.814564	1.44E-51	-1.69E-46
MS.gene060297	MS.gene86350	0.803653	3.15E-49	-1.69E-46
MS.gene060586	MS.gene86350	0.807783	4.27E-50	-1.69E-46
MS.gene060619	MS.gene86350	0.826439	2.71E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86350.t1	MTR_2g103160	98.013	151	3	0	1	151	88	238	8.92e-109	309
MS.gene86350.t1	MTR_6g004800	94.040	151	8	1	1	151	88	237	2.18e-104	298

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86350.t1	AT1G31817	58.553	152	62	1	1	151	163	314	6.88e-63	195

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGCAGTCTGCAGCCATTA+TGG	0.260348	2.3:+1745896	None:intergenic
AATCAGGGCAAAAGCTTTCT+AGG	0.277751	2.3:-1746116	MS.gene86350:CDS
CCCTGATTTCATATCTTTCA+AGG	0.313093	2.3:+1746131	None:intergenic
GGACGAAGATCTAGAAGTTT+GGG	0.313205	2.3:-1746063	MS.gene86350:CDS
AACTAGATACGTCGTTTCTT+TGG	0.319042	2.3:+1745877	None:intergenic
TGGCAGTCTGCAGCCATTAT+GGG	0.322578	2.3:+1745897	None:intergenic
TGGACGAAGATCTAGAAGTT+TGG	0.345854	2.3:-1746064	MS.gene86350:CDS
GAAAATGAAAAGGGTGTTAT+GGG	0.347824	2.3:-1746285	MS.gene86350:CDS
TCCTTGAAAGATATGAAATC+AGG	0.348721	2.3:-1746132	MS.gene86350:CDS
AGAAAATGAAAAGGGTGTTA+TGG	0.348875	2.3:-1746286	MS.gene86350:CDS
GTATCTTCAATGTATACAAT+TGG	0.413544	2.3:+1745931	None:intergenic
GTAAAACTTAGTGGCTCTGC+TGG	0.418753	2.3:-1746156	MS.gene86350:CDS
ACAACTCGTCGTCCCCATAA+TGG	0.428444	2.3:-1745910	MS.gene86350:CDS
AAAGGAAATGTAAAACTTAG+TGG	0.428546	2.3:-1746165	MS.gene86350:CDS
GTTACAGTGACCGATTCTAA+AGG	0.474490	2.3:-1746183	MS.gene86350:CDS
AAGTGTAGAAGAAAATGAAA+AGG	0.475421	2.3:-1746295	MS.gene86350:CDS
AGTGTAGAAGAAAATGAAAA+GGG	0.484709	2.3:-1746294	MS.gene86350:CDS
AGCAATATTAAGCTGGAGAG+AGG	0.511053	2.3:-1745983	MS.gene86350:CDS
AGAATCGGTCACTGTAACAA+AGG	0.511601	2.3:+1746188	None:intergenic
TCTGTTGTCATGAAAGTGAA+TGG	0.517166	2.3:-1746033	MS.gene86350:CDS
TGCTGAAGCAACTGCAGAAG+TGG	0.550397	2.3:-1746088	MS.gene86350:CDS
AAATGAAAAGGGTGTTATGG+GGG	0.550474	2.3:-1746283	MS.gene86350:CDS
AAAGGCAAGCAATATTAAGC+TGG	0.550856	2.3:-1745990	MS.gene86350:CDS
GGCAGTCTGCAGCCATTATG+GGG	0.556364	2.3:+1745898	None:intergenic
CCTTGAAAGATATGAAATCA+GGG	0.577321	2.3:-1746131	MS.gene86350:CDS
GAAGCAACTGCAGAAGTGGT+TGG	0.594231	2.3:-1746084	MS.gene86350:CDS
GCAATATTAAGCTGGAGAGA+GGG	0.596295	2.3:-1745982	MS.gene86350:CDS
AATGAAAAGGGTGTTATGGG+GGG	0.597424	2.3:-1746282	MS.gene86350:CDS
AGGAAGATGGACTTTGTGAG+AGG	0.603497	2.3:-1746318	None:intergenic
AAAATGAAAAGGGTGTTATG+GGG	0.604711	2.3:-1746284	MS.gene86350:CDS
GACGAAGATCTAGAAGTTTG+GGG	0.621557	2.3:-1746062	MS.gene86350:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTACATTTCCTTTAGAAT+CGG	+	chr2.3:1746021-1746040	None:intergenic	20.0%
!	AAAGGAAATGTAAAACTTAG+TGG	-	chr2.3:1746026-1746045	MS.gene86350:CDS	25.0%
!	AAGTGTAGAAGAAAATGAAA+AGG	-	chr2.3:1745896-1745915	MS.gene86350:CDS	25.0%
!	AGTGTAGAAGAAAATGAAAA+GGG	-	chr2.3:1745897-1745916	MS.gene86350:CDS	25.0%
!	GTATCTTCAATGTATACAAT+TGG	+	chr2.3:1746263-1746282	None:intergenic	25.0%
!!!	TGAATGGATTTACTCATTTT+CGG	-	chr2.3:1746174-1746193	MS.gene86350:CDS	25.0%
	CCTTGAAAGATATGAAATCA+GGG	-	chr2.3:1746060-1746079	MS.gene86350:CDS	30.0%
	TCCTTGAAAGATATGAAATC+AGG	-	chr2.3:1746059-1746078	MS.gene86350:CDS	30.0%
!!	AAAATGAAAAGGGTGTTATG+GGG	-	chr2.3:1745907-1745926	MS.gene86350:CDS	30.0%
!!	AGAAAATGAAAAGGGTGTTA+TGG	-	chr2.3:1745905-1745924	MS.gene86350:CDS	30.0%
!!	GAAAATGAAAAGGGTGTTAT+GGG	-	chr2.3:1745906-1745925	MS.gene86350:CDS	30.0%
	AAAGGCAAGCAATATTAAGC+TGG	-	chr2.3:1746201-1746220	MS.gene86350:CDS	35.0%
	CCCTGATTTCATATCTTTCA+AGG	+	chr2.3:1746063-1746082	None:intergenic	35.0%
	TCTGTTGTCATGAAAGTGAA+TGG	-	chr2.3:1746158-1746177	MS.gene86350:CDS	35.0%
!	ATGGATTTACTCATTTTCGG+AGG	-	chr2.3:1746177-1746196	MS.gene86350:CDS	35.0%
!	TTACTCATTTTCGGAGGAAA+AGG	-	chr2.3:1746183-1746202	MS.gene86350:CDS	35.0%
!!	AAATGAAAAGGGTGTTATGG+GGG	-	chr2.3:1745908-1745927	MS.gene86350:CDS	35.0%
	AATCAGGGCAAAAGCTTTCT+AGG	-	chr2.3:1746075-1746094	MS.gene86350:CDS	40.0%
	AGAATCGGTCACTGTAACAA+AGG	+	chr2.3:1746006-1746025	None:intergenic	40.0%
	AGCAATATTAAGCTGGAGAG+AGG	-	chr2.3:1746208-1746227	MS.gene86350:CDS	40.0%
	GACGAAGATCTAGAAGTTTG+GGG	-	chr2.3:1746129-1746148	MS.gene86350:CDS	40.0%
	GCAATATTAAGCTGGAGAGA+GGG	-	chr2.3:1746209-1746228	MS.gene86350:CDS	40.0%
	GGACGAAGATCTAGAAGTTT+GGG	-	chr2.3:1746128-1746147	MS.gene86350:CDS	40.0%
	TGGACGAAGATCTAGAAGTT+TGG	-	chr2.3:1746127-1746146	MS.gene86350:CDS	40.0%
!	GTTACAGTGACCGATTCTAA+AGG	-	chr2.3:1746008-1746027	MS.gene86350:CDS	40.0%
!!	AATGAAAAGGGTGTTATGGG+GGG	-	chr2.3:1745909-1745928	MS.gene86350:CDS	40.0%
!	GAGGGATTTTCTGATTCTCG+AGG	-	chr2.3:1746227-1746246	MS.gene86350:CDS	45.0%
!	GTAAAACTTAGTGGCTCTGC+TGG	-	chr2.3:1746035-1746054	MS.gene86350:CDS	45.0%
	ACAACTCGTCGTCCCCATAA+TGG	-	chr2.3:1746281-1746300	MS.gene86350:CDS	50.0%
	GAAGCAACTGCAGAAGTGGT+TGG	-	chr2.3:1746107-1746126	MS.gene86350:CDS	50.0%
	TGCTGAAGCAACTGCAGAAG+TGG	-	chr2.3:1746103-1746122	MS.gene86350:CDS	50.0%
	TGGCAGTCTGCAGCCATTAT+GGG	+	chr2.3:1746297-1746316	None:intergenic	50.0%
	TTGGCAGTCTGCAGCCATTA+TGG	+	chr2.3:1746298-1746317	None:intergenic	50.0%
	GGCAGTCTGCAGCCATTATG+GGG	+	chr2.3:1746296-1746315	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	1745879	1746334	1745879	ID=MS.gene86350
chr2.3	mRNA	1745879	1746334	1745879	ID=MS.gene86350.t1;Parent=MS.gene86350
chr2.3	exon	1745879	1746334	1745879	ID=MS.gene86350.t1.exon1;Parent=MS.gene86350.t1
chr2.3	CDS	1745879	1746334	1745879	ID=cds.MS.gene86350.t1;Parent=MS.gene86350.t1

Gene Sequence

>MS.gene86350

ATGGACTTTGTGAGAGGAAGTGTAGAAGAAAATGAAAAGGGTGTTATGGGGGGTTATCTATACAATCAGTATCATTTTCAGCATGATGCTGATTTTGTTCATATCAAGATGCGTCGTAACAATACCTTTGTTACAGTGACCGATTCTAAAGGAAATGTAAAACTTAGTGGCTCTGCTGGTTCCTTGAAAGATATGAAATCAGGGCAAAAGCTTTCTAGGTATGCTGCTGAAGCAACTGCAGAAGTGGTTGGACGAAGATCTAGAAGTTTGGGGTTGAAATCTGTTGTCATGAAAGTGAATGGATTTACTCATTTTCGGAGGAAAAGGCAAGCAATATTAAGCTGGAGAGAGGGATTTTCTGATTCTCGAGGAGATAGAAATCCAATTGTATACATTGAAGATACAACTCGTCGTCCCCATAATGGCTGCAGACTGCCAAAGAAACGACGTATCTAG

Protein sequence

>MS.gene86350.t1

MDFVRGSVEENEKGVMGGYLYNQYHFQHDADFVHIKMRRNNTFVTVTDSKGNVKLSGSAGSLKDMKSGQKLSRYAAEATAEVVGRRSRSLGLKSVVMKVNGFTHFRRKRQAILSWREGFSDSRGDRNPIVYIEDTTRRPHNGCRLPKKRRI