Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87377.t1 | XP_013446424.1 | 88 | 490 | 49 | 4 | 1 | 480 | 1 | 490 | 8.10E-201 | 709.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87377.t1 | O64827 | 26.1 | 517 | 269 | 11 | 43 | 448 | 409 | 923 | 8.9e-31 | 136.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87377.t1 | A0A072TTG4 | 88.0 | 490 | 49 | 4 | 1 | 480 | 1 | 490 | 5.9e-201 | 709.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene87377.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene87377 | 0.803706 | 3.07E-49 | -1.69E-46 |
| MS.gene049272 | MS.gene87377 | 0.806901 | 6.57E-50 | -1.69E-46 |
| MS.gene049343 | MS.gene87377 | 0.80307 | 4.15E-49 | -1.69E-46 |
| MS.gene049824 | MS.gene87377 | 0.805938 | 1.05E-49 | -1.69E-46 |
| MS.gene049825 | MS.gene87377 | 0.834975 | 2.20E-56 | -1.69E-46 |
| MS.gene049873 | MS.gene87377 | 0.839717 | 1.34E-57 | -1.69E-46 |
| MS.gene050026 | MS.gene87377 | 0.801941 | 7.10E-49 | -1.69E-46 |
| MS.gene050156 | MS.gene87377 | 0.828552 | 8.44E-55 | -1.69E-46 |
| MS.gene050201 | MS.gene87377 | 0.822154 | 2.76E-53 | -1.69E-46 |
| MS.gene050227 | MS.gene87377 | 0.81123 | 7.77E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene87377 | 0.819432 | 1.17E-52 | -1.69E-46 |
| MS.gene050237 | MS.gene87377 | 0.802295 | 6.01E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene87377 | 0.829062 | 6.35E-55 | -1.69E-46 |
| MS.gene050355 | MS.gene87377 | 0.822287 | 2.57E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene87377 | 0.802731 | 4.88E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene87377 | 0.860371 | 2.16E-63 | -1.69E-46 |
| MS.gene050721 | MS.gene87377 | 0.859444 | 4.12E-63 | -1.69E-46 |
| MS.gene050724 | MS.gene87377 | 0.827168 | 1.82E-54 | -1.69E-46 |
| MS.gene050780 | MS.gene87377 | 0.824631 | 7.27E-54 | -1.69E-46 |
| MS.gene050799 | MS.gene87377 | 0.819705 | 1.01E-52 | -1.69E-46 |
| MS.gene050824 | MS.gene87377 | 0.814439 | 1.54E-51 | -1.69E-46 |
| MS.gene050844 | MS.gene87377 | 0.802096 | 6.60E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene87377 | 0.802427 | 5.64E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene87377 | 0.823527 | 1.32E-53 | -1.69E-46 |
| MS.gene051265 | MS.gene87377 | 0.800794 | 1.22E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene87377 | 0.802456 | 5.56E-49 | -1.69E-46 |
| MS.gene051935 | MS.gene87377 | 0.800385 | 1.48E-48 | -1.69E-46 |
| MS.gene052020 | MS.gene87377 | 0.804075 | 2.57E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene87377 | 0.82374 | 1.18E-53 | -1.69E-46 |
| MS.gene052190 | MS.gene87377 | 0.826248 | 3.01E-54 | -1.69E-46 |
| MS.gene052208 | MS.gene87377 | 0.827963 | 1.17E-54 | -1.69E-46 |
| MS.gene052333 | MS.gene87377 | 0.812952 | 3.27E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene87377 | -0.848163 | 7.30E-60 | -1.69E-46 |
| MS.gene052519 | MS.gene87377 | 0.823663 | 1.23E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene87377 | 0.808391 | 3.17E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene87377 | 0.817869 | 2.64E-52 | -1.69E-46 |
| MS.gene052586 | MS.gene87377 | 0.818472 | 1.93E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene87377 | 0.808481 | 3.03E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene87377 | 0.807022 | 6.19E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene87377 | 0.825554 | 4.40E-54 | -1.69E-46 |
| MS.gene053006 | MS.gene87377 | 0.817206 | 3.72E-52 | -1.69E-46 |
| MS.gene053054 | MS.gene87377 | -0.818289 | 2.12E-52 | -1.69E-46 |
| MS.gene053221 | MS.gene87377 | 0.83657 | 8.66E-57 | -1.69E-46 |
| MS.gene053226 | MS.gene87377 | 0.819803 | 9.59E-53 | -1.69E-46 |
| MS.gene053294 | MS.gene87377 | 0.808278 | 3.35E-50 | -1.69E-46 |
| MS.gene05365 | MS.gene87377 | 0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene053966 | MS.gene87377 | 0.810712 | 1.01E-50 | -1.69E-46 |
| MS.gene05414 | MS.gene87377 | 0.821956 | 3.07E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene87377 | 0.834914 | 2.28E-56 | -1.69E-46 |
| MS.gene054291 | MS.gene87377 | -0.811508 | 6.76E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene87377 | 0.818794 | 1.63E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene87377 | 0.83966 | 1.39E-57 | -1.69E-46 |
| MS.gene055818 | MS.gene87377 | 0.847339 | 1.23E-59 | -1.69E-46 |
| MS.gene056091 | MS.gene87377 | 0.830214 | 3.33E-55 | -1.69E-46 |
| MS.gene056366 | MS.gene87377 | 0.802325 | 5.92E-49 | -1.69E-46 |
| MS.gene056373 | MS.gene87377 | 0.803348 | 3.64E-49 | -1.69E-46 |
| MS.gene056498 | MS.gene87377 | 0.803154 | 3.99E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene87377 | 0.831406 | 1.70E-55 | -1.69E-46 |
| MS.gene05690 | MS.gene87377 | 0.807393 | 5.17E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene87377 | 0.845966 | 2.92E-59 | -1.69E-46 |
| MS.gene057027 | MS.gene87377 | 0.818367 | 2.03E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene87377 | 0.817593 | 3.04E-52 | -1.69E-46 |
| MS.gene057314 | MS.gene87377 | 0.804259 | 2.35E-49 | -1.69E-46 |
| MS.gene057414 | MS.gene87377 | 0.800979 | 1.12E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene87377 | 0.806757 | 7.04E-50 | -1.69E-46 |
| MS.gene05795 | MS.gene87377 | 0.801424 | 9.06E-49 | -1.69E-46 |
| MS.gene057967 | MS.gene87377 | 0.821493 | 3.92E-53 | -1.69E-46 |
| MS.gene057980 | MS.gene87377 | 0.818187 | 2.23E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene87377 | 0.830856 | 2.32E-55 | -1.69E-46 |
| MS.gene058625 | MS.gene87377 | 0.834989 | 2.18E-56 | -1.69E-46 |
| MS.gene058635 | MS.gene87377 | 0.800734 | 1.25E-48 | -1.69E-46 |
| MS.gene058636 | MS.gene87377 | 0.827907 | 1.21E-54 | -1.69E-46 |
| MS.gene058829 | MS.gene87377 | 0.828833 | 7.21E-55 | -1.69E-46 |
| MS.gene058979 | MS.gene87377 | 0.816368 | 5.73E-52 | -1.69E-46 |
| MS.gene059249 | MS.gene87377 | 0.801532 | 8.62E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene87377 | 0.801634 | 8.21E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene87377 | 0.841492 | 4.60E-58 | -1.69E-46 |
| MS.gene059789 | MS.gene87377 | 0.83151 | 1.60E-55 | -1.69E-46 |
| MS.gene060322 | MS.gene87377 | 0.812808 | 3.52E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene87377 | 0.810903 | 9.15E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene87377 | 0.875095 | 4.04E-68 | -1.69E-46 |
| MS.gene060586 | MS.gene87377 | 0.808635 | 2.81E-50 | -1.69E-46 |
| MS.gene060619 | MS.gene87377 | 0.803528 | 3.34E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene87377 | -0.813283 | 2.77E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene87377 | -0.862792 | 3.94E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87377.t1 | MTR_8g078700 | 87.143 | 490 | 53 | 4 | 1 | 480 | 1 | 490 | 0.0 | 870 |
| MS.gene87377.t1 | MTR_8g078490 | 62.039 | 461 | 148 | 7 | 33 | 475 | 387 | 838 | 0.0 | 547 |
| MS.gene87377.t1 | MTR_4g132610 | 59.236 | 314 | 103 | 3 | 39 | 345 | 367 | 662 | 3.19e-114 | 361 |
| MS.gene87377.t1 | MTR_5g018850 | 58.271 | 266 | 100 | 5 | 218 | 475 | 817 | 1079 | 5.10e-91 | 303 |
| MS.gene87377.t1 | MTR_5g018850 | 41.096 | 146 | 79 | 1 | 56 | 194 | 507 | 652 | 3.31e-26 | 113 |
| MS.gene87377.t1 | MTR_8g078505 | 59.350 | 246 | 52 | 2 | 20 | 228 | 6 | 240 | 4.49e-87 | 277 |
| MS.gene87377.t1 | MTR_4g132670 | 42.276 | 246 | 74 | 4 | 78 | 322 | 210 | 388 | 3.05e-50 | 176 |
| MS.gene87377.t1 | MTR_4g132670 | 56.897 | 58 | 25 | 0 | 58 | 115 | 55 | 112 | 6.29e-14 | 73.9 |
| MS.gene87377.t1 | MTR_8g442480 | 51.685 | 89 | 40 | 2 | 63 | 148 | 2 | 90 | 4.38e-20 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87377.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 722 | 916 | 3.68e-55 | 199 |
| MS.gene87377.t1 | AT2G23740 | 41.353 | 133 | 70 | 2 | 45 | 169 | 404 | 536 | 5.41e-21 | 97.4 |
| MS.gene87377.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.05e-55 | 199 |
| MS.gene87377.t1 | AT2G23740 | 41.353 | 133 | 70 | 2 | 45 | 169 | 411 | 543 | 5.98e-21 | 97.4 |
| MS.gene87377.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.05e-55 | 199 |
| MS.gene87377.t1 | AT2G23740 | 41.353 | 133 | 70 | 2 | 45 | 169 | 411 | 543 | 5.98e-21 | 97.4 |
| MS.gene87377.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.05e-55 | 199 |
| MS.gene87377.t1 | AT2G23740 | 41.353 | 133 | 70 | 2 | 45 | 169 | 411 | 543 | 5.98e-21 | 97.4 |
| MS.gene87377.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.56e-55 | 199 |
| MS.gene87377.t1 | AT2G23740 | 41.353 | 133 | 70 | 2 | 45 | 169 | 411 | 543 | 5.38e-21 | 97.4 |
| MS.gene87377.t1 | AT2G23740 | 50.254 | 197 | 96 | 2 | 252 | 448 | 729 | 923 | 4.56e-55 | 199 |
| MS.gene87377.t1 | AT2G23740 | 41.353 | 133 | 70 | 2 | 45 | 169 | 411 | 543 | 5.38e-21 | 97.4 |
Find 93 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTCAGATAGAGCCTTTC+TGG | 0.146763 | 8.3:-25608511 | None:intergenic |
| TCCAAACCTCTTTCAAATTT+AGG | 0.154205 | 8.3:-25608324 | None:intergenic |
| GGCGAAACGCTCAAGCTTTC+CGG | 0.154625 | 8.3:+25607646 | MS.gene87377:CDS |
| ATTCCTTGTGGTTGTCATTT+TGG | 0.199979 | 8.3:+25607958 | MS.gene87377:CDS |
| TTGATCTCCAATGGACTTTC+CGG | 0.248032 | 8.3:-25607571 | None:intergenic |
| CCAGCCTCATGCAGATAAAT+TGG | 0.273277 | 8.3:+25608440 | MS.gene87377:CDS |
| TTGGAAATATAGAGGAATTA+TGG | 0.274132 | 8.3:+25607977 | MS.gene87377:CDS |
| GGCAAATAAGTTGCTTGAAA+TGG | 0.279485 | 8.3:+25608404 | MS.gene87377:CDS |
| CCTCTTTCAAATTTAGGATT+AGG | 0.281407 | 8.3:-25608318 | None:intergenic |
| TTCTTCGCAAAGAACATCTT+TGG | 0.285228 | 8.3:+25607625 | MS.gene87377:CDS |
| ATGCAAATATGTTCTTCATT+AGG | 0.299340 | 8.3:-25607130 | None:intergenic |
| CTTGACATTGTCGACCCTTT+TGG | 0.303194 | 8.3:-25607237 | None:intergenic |
| TCCTAAATTTGAAAGAGGTT+TGG | 0.365758 | 8.3:+25608323 | MS.gene87377:CDS |
| TGTACGAGATATTGAATTTG+AGG | 0.372830 | 8.3:-25607476 | None:intergenic |
| CCATCATCAAGCTGCTCATA+TGG | 0.373746 | 8.3:+25608212 | MS.gene87377:CDS |
| GCCTGTCCACTGATCATTAC+CGG | 0.388651 | 8.3:-25607665 | None:intergenic |
| GCAGTTGTACCTCCACAAAA+TGG | 0.390067 | 8.3:-25607370 | None:intergenic |
| TCCAATGGTTGCCAAGTGTT+TGG | 0.392168 | 8.3:-25607783 | None:intergenic |
| GCAAATAAGTTGCTTGAAAT+GGG | 0.399729 | 8.3:+25608405 | MS.gene87377:CDS |
| AAGAGACACTATGGGCAGTT+TGG | 0.400556 | 8.3:+25607910 | MS.gene87377:CDS |
| AGTCTTTGCAAATCAGGCTC+TGG | 0.401375 | 8.3:-25607534 | None:intergenic |
| CGATCTAGCCAGCAGGCGCT+TGG | 0.413605 | 8.3:+25608239 | MS.gene87377:CDS |
| ACACCAAGTCTTTGCAAATC+AGG | 0.417723 | 8.3:-25607540 | None:intergenic |
| GGAAGAATCTCAATGCTTAG+AGG | 0.420258 | 8.3:+25608461 | MS.gene87377:CDS |
| AGTTATCGAAAGCTTAAATT+CGG | 0.424504 | 8.3:-25608025 | None:intergenic |
| CCTAATCCTAAATTTGAAAG+AGG | 0.427935 | 8.3:+25608318 | MS.gene87377:CDS |
| GTTTGGAAGAAGCATCAAAT+AGG | 0.428853 | 8.3:+25608340 | MS.gene87377:CDS |
| AGACTTGGTGTTTGTACATC+CGG | 0.429428 | 8.3:+25607552 | MS.gene87377:CDS |
| ATCGAAAGCTTAAATTCGGC+AGG | 0.430592 | 8.3:-25608021 | None:intergenic |
| TTTCATGACTTTCCATCCAA+TGG | 0.430775 | 8.3:-25607798 | None:intergenic |
| CCAATTTATCTGCATGAGGC+TGG | 0.431124 | 8.3:-25608440 | None:intergenic |
| ACTCTTTGCCAAGCGCCTGC+TGG | 0.443750 | 8.3:-25608247 | None:intergenic |
| CAGTGACAGTTCATCTGTAA+TGG | 0.451819 | 8.3:+25607195 | MS.gene87377:CDS |
| GTTCGCGGAAGTTTAGTTGC+AGG | 0.454226 | 8.3:+25608148 | MS.gene87377:CDS |
| TAATTTCAAGAATAGAAATC+AGG | 0.462085 | 8.3:+25608383 | MS.gene87377:CDS |
| TTCTCCTAAAATGATTGAGC+AGG | 0.465200 | 8.3:+25608080 | MS.gene87377:CDS |
| TGATGATGGCGACCGAGATC+AGG | 0.469915 | 8.3:-25608198 | None:intergenic |
| GTCTTTGCAAATCAGGCTCT+GGG | 0.470035 | 8.3:-25607533 | None:intergenic |
| ACCAAACACTTGGCAACCAT+TGG | 0.477256 | 8.3:+25607782 | MS.gene87377:CDS |
| GACTCTTATAGAAAACCAAA+AGG | 0.481717 | 8.3:+25607222 | MS.gene87377:CDS |
| CCATATGAGCAGCTTGATGA+TGG | 0.488631 | 8.3:-25608212 | None:intergenic |
| TGAGAGTGCTGTTAAGTGTA+AGG | 0.490329 | 8.3:+25607735 | MS.gene87377:CDS |
| TTGGATGGAAAGTCATGAAA+AGG | 0.491304 | 8.3:+25607801 | MS.gene87377:CDS |
| TCATCATTGACGATTGACTC+TGG | 0.492547 | 8.3:-25607595 | None:intergenic |
| AGTGAAGGAATCAAAACAAA+AGG | 0.493161 | 8.3:-25607857 | None:intergenic |
| CATGTTCAGAAGAGACACTA+TGG | 0.495523 | 8.3:+25607901 | MS.gene87377:CDS |
| ACATCTGCACAAAGCTGAGA+TGG | 0.500567 | 8.3:+25607447 | MS.gene87377:CDS |
| GAGATATTGAATTTGAGGTC+TGG | 0.501478 | 8.3:-25607471 | None:intergenic |
| CAGTGCGTGAGAACCGCAAT+TGG | 0.508352 | 8.3:+25607283 | MS.gene87377:CDS |
| GTTTCGTTTATCTACCAGAA+AGG | 0.508712 | 8.3:+25608497 | MS.gene87377:CDS |
| TATGCTCAGAGACTAAAATC+TGG | 0.515386 | 8.3:+25608282 | MS.gene87377:CDS |
| GAGCCTGATTTGCAAAGACT+TGG | 0.521361 | 8.3:+25607537 | MS.gene87377:CDS |
| TTCAAATTTAGGATTAGGAC+GGG | 0.522702 | 8.3:-25608313 | None:intergenic |
| ATTAGCTTTAGCCCTTCTCC+TGG | 0.523241 | 8.3:-25608364 | None:intergenic |
| TCCGGTAATGATCAGTGGAC+AGG | 0.523358 | 8.3:+25607664 | MS.gene87377:CDS |
| TGGAGCGCGATCTAGCCAGC+AGG | 0.523628 | 8.3:+25608232 | MS.gene87377:CDS |
| AGCAGGCGCTTGGCAAAGAG+TGG | 0.523735 | 8.3:+25608249 | MS.gene87377:CDS |
| ACAAATCAAGGAGCAGCTTA+CGG | 0.524363 | 8.3:+25607106 | MS.gene87377:CDS |
| CAATATCTCGTACATTAAAG+AGG | 0.524991 | 8.3:+25607485 | MS.gene87377:CDS |
| GACTCTGGATTGATCTCCAA+TGG | 0.529884 | 8.3:-25607580 | None:intergenic |
| CTGAGAATCGAAGTGGTTCG+CGG | 0.530378 | 8.3:+25608133 | MS.gene87377:CDS |
| GTGTATATTGTTACCAATTG+CGG | 0.530995 | 8.3:-25607296 | None:intergenic |
| TGTACATCCGGAAAGTCCAT+TGG | 0.533385 | 8.3:+25607564 | MS.gene87377:CDS |
| CATCAAATAGGACCAGGAGA+AGG | 0.534319 | 8.3:+25608352 | MS.gene87377:CDS |
| TTTGATTTACTACCTGATCT+CGG | 0.536142 | 8.3:+25608186 | MS.gene87377:CDS |
| AACACTTGGCAACCATTGGA+TGG | 0.536290 | 8.3:+25607786 | MS.gene87377:CDS |
| TTTCAAATTTAGGATTAGGA+CGG | 0.543514 | 8.3:-25608314 | None:intergenic |
| ATCAAATAGGACCAGGAGAA+GGG | 0.546017 | 8.3:+25608353 | MS.gene87377:CDS |
| TTTATCTGCATGAGGCTGGA+TGG | 0.546716 | 8.3:-25608436 | None:intergenic |
| TCTTCCAATTTATCTGCATG+AGG | 0.563398 | 8.3:-25608444 | None:intergenic |
| GATTGCTCTGAGACAAATCA+AGG | 0.567116 | 8.3:+25607094 | MS.gene87377:CDS |
| GACAGTTCATCTGTAATGGA+AGG | 0.570153 | 8.3:+25607199 | MS.gene87377:CDS |
| ACCAGAAAGGCTCTATCTGA+AGG | 0.579442 | 8.3:+25608510 | MS.gene87377:CDS |
| CATCTGCACAAAGCTGAGAT+GGG | 0.579947 | 8.3:+25607448 | MS.gene87377:CDS |
| ACTCTTATAGAAAACCAAAA+GGG | 0.580021 | 8.3:+25607223 | MS.gene87377:CDS |
| TGGACAGGCTCTCCTAGCTA+TGG | 0.583120 | 8.3:+25607679 | MS.gene87377:CDS |
| TTATGATCATTACCATAGCT+AGG | 0.583706 | 8.3:-25607691 | None:intergenic |
| TGTGGAGGTACAACTGCTGC+TGG | 0.586893 | 8.3:+25607376 | MS.gene87377:CDS |
| TCTCCTAAAATGATTGAGCA+GGG | 0.608885 | 8.3:+25608081 | MS.gene87377:CDS |
| GAAGCACAGTGGTTGTTCAG+AGG | 0.608970 | 8.3:+25607823 | MS.gene87377:CDS |
| GATAATTCTGAGAATCGAAG+TGG | 0.614192 | 8.3:+25608126 | MS.gene87377:CDS |
| AGCTTTCCGGTAATGATCAG+TGG | 0.616263 | 8.3:+25607659 | MS.gene87377:CDS |
| CATATATCGAAGACAAGGGA+AGG | 0.617551 | 8.3:+25607260 | MS.gene87377:CDS |
| GAGCAACTAACATTGTCTAC+CGG | 0.626990 | 8.3:+25608051 | MS.gene87377:CDS |
| TCAAGCATATATCGAAGACA+AGG | 0.630094 | 8.3:+25607255 | MS.gene87377:CDS |
| ACAGTTCATCTGTAATGGAA+GGG | 0.632090 | 8.3:+25607200 | MS.gene87377:CDS |
| AAGAAGCATCAAATAGGACC+AGG | 0.634670 | 8.3:+25608346 | MS.gene87377:CDS |
| ATGTTCAGAAGAGACACTAT+GGG | 0.654163 | 8.3:+25607902 | MS.gene87377:CDS |
| TCGAAAGCTTAAATTCGGCA+GGG | 0.657128 | 8.3:-25608020 | None:intergenic |
| AAAGCTGAACAAAATTGCAG+TGG | 0.668916 | 8.3:+25607508 | MS.gene87377:CDS |
| CAAGCATATATCGAAGACAA+GGG | 0.692685 | 8.3:+25607256 | MS.gene87377:CDS |
| TTTCCAAAATGACAACCACA+AGG | 0.715599 | 8.3:-25607961 | None:intergenic |
| GTCATGAAAAGGAAGCACAG+TGG | 0.767507 | 8.3:+25607812 | MS.gene87377:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCTTAAGTAAAAAAGAA+AGG | + | chr8.3:25607329-25607348 | MS.gene87377:CDS | 15.0% |
| !! | TAATTTCAAGAATAGAAATC+AGG | + | chr8.3:25608383-25608402 | MS.gene87377:CDS | 20.0% |
| !! | TTCTTACAAAAAGATAATCT+GGG | - | chr8.3:25607427-25607446 | None:intergenic | 20.0% |
| !! | TTTCTTACAAAAAGATAATC+TGG | - | chr8.3:25607428-25607447 | None:intergenic | 20.0% |
| ! | ACTCTTATAGAAAACCAAAA+GGG | + | chr8.3:25607223-25607242 | MS.gene87377:CDS | 25.0% |
| ! | AGTTATCGAAAGCTTAAATT+CGG | - | chr8.3:25608028-25608047 | None:intergenic | 25.0% |
| ! | ATGCAAATATGTTCTTCATT+AGG | - | chr8.3:25607133-25607152 | None:intergenic | 25.0% |
| ! | CTTCACTAACAAAAAACTAT+TGG | + | chr8.3:25607873-25607892 | MS.gene87377:CDS | 25.0% |
| ! | TTGGAAATATAGAGGAATTA+TGG | + | chr8.3:25607977-25607996 | MS.gene87377:CDS | 25.0% |
| !! | TTTCAAATTTAGGATTAGGA+CGG | - | chr8.3:25608317-25608336 | None:intergenic | 25.0% |
| !!! | CAATAGTTTTTTGTTAGTGA+AGG | - | chr8.3:25607875-25607894 | None:intergenic | 25.0% |
| !!! | TTGTCATTTTGGAAATATAG+AGG | + | chr8.3:25607969-25607988 | MS.gene87377:CDS | 25.0% |
| ACAAAAAGATAATCTGGGAA+GGG | - | chr8.3:25607422-25607441 | None:intergenic | 30.0% | |
| AGTGAAGGAATCAAAACAAA+AGG | - | chr8.3:25607860-25607879 | None:intergenic | 30.0% | |
| CAATATCTCGTACATTAAAG+AGG | + | chr8.3:25607485-25607504 | MS.gene87377:CDS | 30.0% | |
| CCTAATCCTAAATTTGAAAG+AGG | + | chr8.3:25608318-25608337 | MS.gene87377:CDS | 30.0% | |
| GACTCTTATAGAAAACCAAA+AGG | + | chr8.3:25607222-25607241 | MS.gene87377:CDS | 30.0% | |
| GCAAATAAGTTGCTTGAAAT+GGG | + | chr8.3:25608405-25608424 | MS.gene87377:CDS | 30.0% | |
| GTGTATATTGTTACCAATTG+CGG | - | chr8.3:25607299-25607318 | None:intergenic | 30.0% | |
| TACAAAAAGATAATCTGGGA+AGG | - | chr8.3:25607423-25607442 | None:intergenic | 30.0% | |
| TCCAAACCTCTTTCAAATTT+AGG | - | chr8.3:25608327-25608346 | None:intergenic | 30.0% | |
| TCCTAAATTTGAAAGAGGTT+TGG | + | chr8.3:25608323-25608342 | MS.gene87377:CDS | 30.0% | |
| TGTACGAGATATTGAATTTG+AGG | - | chr8.3:25607479-25607498 | None:intergenic | 30.0% | |
| TTATGATCATTACCATAGCT+AGG | - | chr8.3:25607694-25607713 | None:intergenic | 30.0% | |
| ! | CCTCTTTCAAATTTAGGATT+AGG | - | chr8.3:25608321-25608340 | None:intergenic | 30.0% |
| ! | CTTTTTCTATGTATTCCTTG+TGG | + | chr8.3:25607946-25607965 | MS.gene87377:CDS | 30.0% |
| ! | TTCAAATTTAGGATTAGGAC+GGG | - | chr8.3:25608316-25608335 | None:intergenic | 30.0% |
| ! | TTTGATTTACTACCTGATCT+CGG | + | chr8.3:25608186-25608205 | MS.gene87377:CDS | 30.0% |
| ! | TTTTCTGATAACCAAACACT+TGG | + | chr8.3:25607772-25607791 | MS.gene87377:CDS | 30.0% |
| AAAGCTGAACAAAATTGCAG+TGG | + | chr8.3:25607508-25607527 | MS.gene87377:CDS | 35.0% | |
| ATGTTCAGAAGAGACACTAT+GGG | + | chr8.3:25607902-25607921 | MS.gene87377:CDS | 35.0% | |
| CAAGCATATATCGAAGACAA+GGG | + | chr8.3:25607256-25607275 | MS.gene87377:CDS | 35.0% | |
| GGCAAATAAGTTGCTTGAAA+TGG | + | chr8.3:25608404-25608423 | MS.gene87377:CDS | 35.0% | |
| GTTTCGTTTATCTACCAGAA+AGG | + | chr8.3:25608497-25608516 | MS.gene87377:CDS | 35.0% | |
| TATGCTCAGAGACTAAAATC+TGG | + | chr8.3:25608282-25608301 | MS.gene87377:CDS | 35.0% | |
| TCAAGCATATATCGAAGACA+AGG | + | chr8.3:25607255-25607274 | MS.gene87377:CDS | 35.0% | |
| TCTCCTAAAATGATTGAGCA+GGG | + | chr8.3:25608081-25608100 | MS.gene87377:CDS | 35.0% | |
| TCTTCCAATTTATCTGCATG+AGG | - | chr8.3:25608447-25608466 | None:intergenic | 35.0% | |
| TTCTCCTAAAATGATTGAGC+AGG | + | chr8.3:25608080-25608099 | MS.gene87377:CDS | 35.0% | |
| TTCTTCGCAAAGAACATCTT+TGG | + | chr8.3:25607625-25607644 | MS.gene87377:CDS | 35.0% | |
| TTGGATGGAAAGTCATGAAA+AGG | + | chr8.3:25607801-25607820 | MS.gene87377:CDS | 35.0% | |
| TTTCATGACTTTCCATCCAA+TGG | - | chr8.3:25607801-25607820 | None:intergenic | 35.0% | |
| TTTCCAAAATGACAACCACA+AGG | - | chr8.3:25607964-25607983 | None:intergenic | 35.0% | |
| ! | AAAGCAATCACTCCATTTTG+TGG | + | chr8.3:25607358-25607377 | MS.gene87377:CDS | 35.0% |
| ! | ACAGTTCATCTGTAATGGAA+GGG | + | chr8.3:25607200-25607219 | MS.gene87377:CDS | 35.0% |
| ! | GAGATATTGAATTTGAGGTC+TGG | - | chr8.3:25607474-25607493 | None:intergenic | 35.0% |
| ! | GATAATTCTGAGAATCGAAG+TGG | + | chr8.3:25608126-25608145 | MS.gene87377:CDS | 35.0% |
| ! | TTTCCCTGCTCAATCATTTT+AGG | - | chr8.3:25608087-25608106 | None:intergenic | 35.0% |
| !! | ATTCCTTGTGGTTGTCATTT+TGG | + | chr8.3:25607958-25607977 | MS.gene87377:CDS | 35.0% |
| !! | CATTTTAGGAGAATCATCAC+CGG | - | chr8.3:25608073-25608092 | None:intergenic | 35.0% |
| !! | GTTTGGAAGAAGCATCAAAT+AGG | + | chr8.3:25608340-25608359 | MS.gene87377:CDS | 35.0% |
| ACAAATCAAGGAGCAGCTTA+CGG | + | chr8.3:25607106-25607125 | MS.gene87377:CDS | 40.0% | |
| ACACCAAGTCTTTGCAAATC+AGG | - | chr8.3:25607543-25607562 | None:intergenic | 40.0% | |
| ATCAAATAGGACCAGGAGAA+GGG | + | chr8.3:25608353-25608372 | MS.gene87377:CDS | 40.0% | |
| ATCGAAAGCTTAAATTCGGC+AGG | - | chr8.3:25608024-25608043 | None:intergenic | 40.0% | |
| CAGTGACAGTTCATCTGTAA+TGG | + | chr8.3:25607195-25607214 | MS.gene87377:CDS | 40.0% | |
| CATATATCGAAGACAAGGGA+AGG | + | chr8.3:25607260-25607279 | MS.gene87377:CDS | 40.0% | |
| CATGTTCAGAAGAGACACTA+TGG | + | chr8.3:25607901-25607920 | MS.gene87377:CDS | 40.0% | |
| GAGCAACTAACATTGTCTAC+CGG | + | chr8.3:25608051-25608070 | MS.gene87377:CDS | 40.0% | |
| GGAAGAATCTCAATGCTTAG+AGG | + | chr8.3:25608461-25608480 | MS.gene87377:CDS | 40.0% | |
| TCGAAAGCTTAAATTCGGCA+GGG | - | chr8.3:25608023-25608042 | None:intergenic | 40.0% | |
| ! | GACAGTTCATCTGTAATGGA+AGG | + | chr8.3:25607199-25607218 | MS.gene87377:CDS | 40.0% |
| ! | TCATCATTGACGATTGACTC+TGG | - | chr8.3:25607598-25607617 | None:intergenic | 40.0% |
| ! | TTGATCTCCAATGGACTTTC+CGG | - | chr8.3:25607574-25607593 | None:intergenic | 40.0% |
| !! | AAGAAGCATCAAATAGGACC+AGG | + | chr8.3:25608346-25608365 | MS.gene87377:CDS | 40.0% |
| !! | AGACTTGGTGTTTGTACATC+CGG | + | chr8.3:25607552-25607571 | MS.gene87377:CDS | 40.0% |
| !! | GATTGCTCTGAGACAAATCA+AGG | + | chr8.3:25607094-25607113 | MS.gene87377:CDS | 40.0% |
| !! | TGAGAGTGCTGTTAAGTGTA+AGG | + | chr8.3:25607735-25607754 | MS.gene87377:CDS | 40.0% |
| AACACTTGGCAACCATTGGA+TGG | + | chr8.3:25607786-25607805 | MS.gene87377:CDS | 45.0% | |
| AAGAGACACTATGGGCAGTT+TGG | + | chr8.3:25607910-25607929 | MS.gene87377:CDS | 45.0% | |
| ACATCTGCACAAAGCTGAGA+TGG | + | chr8.3:25607447-25607466 | MS.gene87377:CDS | 45.0% | |
| ACCAAACACTTGGCAACCAT+TGG | + | chr8.3:25607782-25607801 | MS.gene87377:CDS | 45.0% | |
| ACCAGAAAGGCTCTATCTGA+AGG | + | chr8.3:25608510-25608529 | MS.gene87377:CDS | 45.0% | |
| AGCTTTCCGGTAATGATCAG+TGG | + | chr8.3:25607659-25607678 | MS.gene87377:CDS | 45.0% | |
| AGTCTTTGCAAATCAGGCTC+TGG | - | chr8.3:25607537-25607556 | None:intergenic | 45.0% | |
| CATCAAATAGGACCAGGAGA+AGG | + | chr8.3:25608352-25608371 | MS.gene87377:CDS | 45.0% | |
| CATCTGCACAAAGCTGAGAT+GGG | + | chr8.3:25607448-25607467 | MS.gene87377:CDS | 45.0% | |
| CCAATTTATCTGCATGAGGC+TGG | - | chr8.3:25608443-25608462 | None:intergenic | 45.0% | |
| CCAGCCTCATGCAGATAAAT+TGG | + | chr8.3:25608440-25608459 | MS.gene87377:CDS | 45.0% | |
| CCATATGAGCAGCTTGATGA+TGG | - | chr8.3:25608215-25608234 | None:intergenic | 45.0% | |
| CCATCATCAAGCTGCTCATA+TGG | + | chr8.3:25608212-25608231 | MS.gene87377:CDS | 45.0% | |
| GACTCTGGATTGATCTCCAA+TGG | - | chr8.3:25607583-25607602 | None:intergenic | 45.0% | |
| GCAGTTGTACCTCCACAAAA+TGG | - | chr8.3:25607373-25607392 | None:intergenic | 45.0% | |
| GTCTTTGCAAATCAGGCTCT+GGG | - | chr8.3:25607536-25607555 | None:intergenic | 45.0% | |
| TCCAATGGTTGCCAAGTGTT+TGG | - | chr8.3:25607786-25607805 | None:intergenic | 45.0% | |
| TGTACATCCGGAAAGTCCAT+TGG | + | chr8.3:25607564-25607583 | MS.gene87377:CDS | 45.0% | |
| TTTATCTGCATGAGGCTGGA+TGG | - | chr8.3:25608439-25608458 | None:intergenic | 45.0% | |
| ! | CTTGACATTGTCGACCCTTT+TGG | - | chr8.3:25607240-25607259 | None:intergenic | 45.0% |
| ! | GAGCCTGATTTGCAAAGACT+TGG | + | chr8.3:25607537-25607556 | MS.gene87377:CDS | 45.0% |
| ! | GCAATCACTCCATTTTGTGG+AGG | + | chr8.3:25607361-25607380 | MS.gene87377:CDS | 45.0% |
| ! | GTCATGAAAAGGAAGCACAG+TGG | + | chr8.3:25607812-25607831 | MS.gene87377:CDS | 45.0% |
| !! | ATTAGCTTTAGCCCTTCTCC+TGG | - | chr8.3:25608367-25608386 | None:intergenic | 45.0% |
| CTGAGAATCGAAGTGGTTCG+CGG | + | chr8.3:25608133-25608152 | MS.gene87377:CDS | 50.0% | |
| GAAGCACAGTGGTTGTTCAG+AGG | + | chr8.3:25607823-25607842 | MS.gene87377:CDS | 50.0% | |
| GCCTGTCCACTGATCATTAC+CGG | - | chr8.3:25607668-25607687 | None:intergenic | 50.0% | |
| GCCTTCAGATAGAGCCTTTC+TGG | - | chr8.3:25608514-25608533 | None:intergenic | 50.0% | |
| GTTCGCGGAAGTTTAGTTGC+AGG | + | chr8.3:25608148-25608167 | MS.gene87377:CDS | 50.0% | |
| TCCGGTAATGATCAGTGGAC+AGG | + | chr8.3:25607664-25607683 | MS.gene87377:CDS | 50.0% | |
| CAGTGCGTGAGAACCGCAAT+TGG | + | chr8.3:25607283-25607302 | MS.gene87377:CDS | 55.0% | |
| GGCGAAACGCTCAAGCTTTC+CGG | + | chr8.3:25607646-25607665 | MS.gene87377:CDS | 55.0% | |
| TGGACAGGCTCTCCTAGCTA+TGG | + | chr8.3:25607679-25607698 | MS.gene87377:CDS | 55.0% | |
| ! | TGTGGAGGTACAACTGCTGC+TGG | + | chr8.3:25607376-25607395 | MS.gene87377:CDS | 55.0% |
| !! | TGATGATGGCGACCGAGATC+AGG | - | chr8.3:25608201-25608220 | None:intergenic | 55.0% |
| ACTCTTTGCCAAGCGCCTGC+TGG | - | chr8.3:25608250-25608269 | None:intergenic | 60.0% | |
| AGCAGGCGCTTGGCAAAGAG+TGG | + | chr8.3:25608249-25608268 | MS.gene87377:CDS | 60.0% | |
| CGATCTAGCCAGCAGGCGCT+TGG | + | chr8.3:25608239-25608258 | MS.gene87377:CDS | 65.0% | |
| TGGAGCGCGATCTAGCCAGC+AGG | + | chr8.3:25608232-25608251 | MS.gene87377:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 25607076 | 25608551 | 25607076 | ID=MS.gene87377 |
| chr8.3 | mRNA | 25607076 | 25608551 | 25607076 | ID=MS.gene87377.t1;Parent=MS.gene87377 |
| chr8.3 | exon | 25607076 | 25608551 | 25607076 | ID=MS.gene87377.t1.exon1;Parent=MS.gene87377.t1 |
| chr8.3 | CDS | 25607076 | 25608551 | 25607076 | ID=cds.MS.gene87377.t1;Parent=MS.gene87377.t1 |
| Gene Sequence |
| Protein sequence |
