AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017178.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017178.01.T01 MTR_3g102500 98.137 161 3 0 1 161 1 161 6.07e-110 310
MsG0380017178.01.T01 MTR_1g018710 57.851 121 42 3 1 121 1 112 1.12e-37 126
MsG0380017178.01.T01 MTR_1g072250 44.318 88 48 1 36 122 17 104 5.53e-18 80.1
MsG0380017178.01.T01 MTR_1g105040 38.596 114 65 3 26 136 6 117 3.03e-16 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017178.01.T01 AT1G47200 62.500 96 35 1 26 121 50 144 9.04e-38 127
MsG0380017178.01.T01 AT5G43070 47.761 134 61 2 1 126 1 133 6.55e-36 122
MsG0380017178.01.T01 AT5G27940 40.385 104 56 2 27 127 41 141 2.93e-21 84.7
MsG0380017178.01.T01 AT3G63130 39.000 100 59 2 36 133 17 116 2.23e-15 72.8
MsG0380017178.01.T01 AT3G63130 39.000 100 59 2 36 133 17 116 2.23e-15 72.8
MsG0380017178.01.T01 AT5G19320 33.981 103 67 1 27 128 7 109 1.10e-13 68.2
MsG0380017178.01.T01 AT5G19320 33.981 103 67 1 27 128 7 109 1.10e-13 68.2

Find 65 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCGGTGACTCCGCCGTTCC+TGG 0.273963 3:-93038806 MsG0380017178.01.T01:CDS
CATCTTCTTACTGATTTCCT+TGG 0.339205 3:+93038748 None:intergenic
GACGGCGTCGCGGGTGCGTT+GGG 0.359562 3:+93038949 None:intergenic
AACGGTAGGTCTAGCCTTAA+CGG 0.377656 3:+93038718 None:intergenic
AAGGACGGAGGGAGTGGTTA+GGG 0.381916 3:+93038907 None:intergenic
TATGCTGAATCCGGCGGAGG+GGG 0.384348 3:+93038982 None:intergenic
CCGTTCCTGGCGGTGGTGAC+GGG 0.385812 3:-93038793 MsG0380017178.01.T01:CDS
GAAGATGCCTTCTCTGTTGC+CGG 0.386460 3:-93038824 MsG0380017178.01.T01:CDS
GCCGTTCCTGGCGGTGGTGA+CGG 0.396124 3:-93038794 MsG0380017178.01.T01:CDS
AAAGGACGGAGGGAGTGGTT+AGG 0.399888 3:+93038906 None:intergenic
TGATTCTAGCGCCGTTGATA+AGG 0.405504 3:-93038696 MsG0380017178.01.T01:CDS
CCTCCGCCGGATTCAGCATA+TGG 0.414915 3:-93038979 MsG0380017178.01.T01:CDS
GGGCAATGTGCCGTAGCGTT+TGG 0.420216 3:+93038883 None:intergenic
GTTAGAGTCCGATGAGGTTA+CGG 0.425356 3:+93039045 None:intergenic
CCATATGCTGAATCCGGCGG+AGG 0.431909 3:+93038979 None:intergenic
GGCGTCGCGGGTGCGTTGGG+TGG 0.444388 3:+93038952 None:intergenic
TGACGGCGTCGCGGGTGCGT+TGG 0.444623 3:+93038948 None:intergenic
TGACGGGATTGAGATCCTTC+AGG 0.459478 3:-93038777 MsG0380017178.01.T01:CDS
CATATGCTGAATCCGGCGGA+GGG 0.462008 3:+93038980 None:intergenic
GCGAGTGATGACGGCGTCGC+GGG 0.464948 3:+93038940 None:intergenic
GCGTCGCGGGTGCGTTGGGT+GGG 0.477005 3:+93038953 None:intergenic
GGGTGGCCATATGCTGAATC+CGG 0.477190 3:+93038973 None:intergenic
AGCGGAGGCGGCTTCGTCGG+AGG 0.481716 3:+93038862 None:intergenic
TCGCGGGTGCGTTGGGTGGG+TGG 0.491919 3:+93038956 None:intergenic
GCCAGGAACGGCGGAGTCAC+CGG 0.492843 3:+93038805 None:intergenic
AGCAGCGGAGGCGGCTTCGT+CGG 0.495524 3:+93038859 None:intergenic
GAAGATGCTTGAAACCGTTA+AGG 0.498552 3:-93038732 MsG0380017178.01.T01:CDS
GACTCCGCCGTTCCTGGCGG+TGG 0.499089 3:-93038800 MsG0380017178.01.T01:CDS
GCGGAGGCGGCTTCGTCGGA+GGG 0.515254 3:+93038863 None:intergenic
ATGCTGAATCCGGCGGAGGG+GGG 0.517309 3:+93038983 None:intergenic
CTCGATCTGACGAGCAGCGG+AGG 0.518920 3:+93038847 None:intergenic
TTTGGAAAGGACGGAGGGAG+TGG 0.526920 3:+93038901 None:intergenic
CCCGTCACCACCGCCAGGAA+CGG 0.545413 3:+93038793 None:intergenic
TTCGATTAAGCGAGTGATGA+CGG 0.547064 3:+93038931 None:intergenic
ATATGCTGAATCCGGCGGAG+GGG 0.550066 3:+93038981 None:intergenic
GATCCTTCAGGTGTATTCCA+AGG 0.552260 3:-93038765 MsG0380017178.01.T01:CDS
GATCTGACGAGCAGCGGAGG+CGG 0.560720 3:+93038850 None:intergenic
GGAGTCACCGGCAACAGAGA+AGG 0.560862 3:+93038817 None:intergenic
TCAATCCCGTCACCACCGCC+AGG 0.562207 3:+93038788 None:intergenic
TACGGTTGTGACTTCTGAGT+CGG 0.571822 3:+93039063 None:intergenic
TGATAAGGACGCTGCTGCTG+TGG 0.577989 3:-93038681 MsG0380017178.01.T01:CDS
AGCAGCAGCGTCCTTATCAA+CGG 0.578380 3:+93038685 None:intergenic
CTCCGCTGCTCGTCAGATCG+AGG 0.582950 3:-93038846 MsG0380017178.01.T01:CDS
GCCGTAGCGTTTGGAAAGGA+CGG 0.591975 3:+93038892 None:intergenic
AGTCTCCGTCTCGATCTTCG+CGG 0.594787 3:+93038613 None:intergenic
TCAGCAGCATCCTCTGCAGA+AGG 0.602802 3:+93038638 None:intergenic
ATCAACGGCGCTAGAATCAA+CGG 0.603734 3:+93038700 None:intergenic
TTTCCTTGGAATACACCTGA+AGG 0.605904 3:+93038762 None:intergenic
TTCCTCGATCTGACGAGCAG+CGG 0.613011 3:+93038844 None:intergenic
GTCACCACCGCCAGGAACGG+CGG 0.615098 3:+93038796 None:intergenic
AGCGAGTGATGACGGCGTCG+CGG 0.615225 3:+93038939 None:intergenic
AGTCACAACCGTAACCTCAT+CGG 0.620023 3:-93039053 MsG0380017178.01.T01:CDS
TGGCCATATGCTGAATCCGG+CGG 0.621199 3:+93038976 None:intergenic
TGATGATCCTCCTTCTGCAG+AGG 0.625146 3:-93038648 MsG0380017178.01.T01:CDS
ATGTGCCGTAGCGTTTGGAA+AGG 0.630318 3:+93038888 None:intergenic
GCAGCATCCTCTGCAGAAGG+AGG 0.632854 3:+93038641 None:intergenic
TCCGTCCTTTCCAAACGCTA+CGG 0.635061 3:-93038893 MsG0380017178.01.T01:CDS
TAGCGTTTGGAAAGGACGGA+GGG 0.638385 3:+93038896 None:intergenic
GAAGGAGGATCATCAACGGT+CGG 0.638649 3:+93038656 None:intergenic
TGCAGAAGGAGGATCATCAA+CGG 0.659742 3:+93038652 None:intergenic
GTAGCGTTTGGAAAGGACGG+AGG 0.678784 3:+93038895 None:intergenic
TGAATCCGCGAAGATCGAGA+CGG 0.689865 3:-93038618 MsG0380017178.01.T01:CDS
ACGGCGCTAGAATCAACGGT+AGG 0.692601 3:+93038704 None:intergenic
GGTGACTCCGCCGTTCCTGG+CGG 0.703703 3:-93038803 MsG0380017178.01.T01:CDS
AACGGAGTTAGAGTCCGATG+AGG 0.729175 3:+93039039 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATCTTCTTACTGATTTCCT+TGG + Chr3:93038926-93038945 None:intergenic 35.0%
GAAGATGCTTGAAACCGTTA+AGG - Chr3:93038939-93038958 MsG0380017178.01.T01:CDS 40.0%
TTCGATTAAGCGAGTGATGA+CGG + Chr3:93038743-93038762 None:intergenic 40.0%
TTTCCTTGGAATACACCTGA+AGG + Chr3:93038912-93038931 None:intergenic 40.0%
AGTCACAACCGTAACCTCAT+CGG - Chr3:93038618-93038637 MsG0380017178.01.T01:CDS 45.0%
ATCAACGGCGCTAGAATCAA+CGG + Chr3:93038974-93038993 None:intergenic 45.0%
GATCCTTCAGGTGTATTCCA+AGG - Chr3:93038906-93038925 MsG0380017178.01.T01:CDS 45.0%
GTTAGAGTCCGATGAGGTTA+CGG + Chr3:93038629-93038648 None:intergenic 45.0%
TACGGTTGTGACTTCTGAGT+CGG + Chr3:93038611-93038630 None:intergenic 45.0%
TGATTCTAGCGCCGTTGATA+AGG - Chr3:93038975-93038994 MsG0380017178.01.T01:CDS 45.0%
TGCAGAAGGAGGATCATCAA+CGG + Chr3:93039022-93039041 None:intergenic 45.0%
!! AACGGTAGGTCTAGCCTTAA+CGG + Chr3:93038956-93038975 None:intergenic 45.0%
AACGGAGTTAGAGTCCGATG+AGG + Chr3:93038635-93038654 None:intergenic 50.0%
AGCAGCAGCGTCCTTATCAA+CGG + Chr3:93038989-93039008 None:intergenic 50.0%
ATGTGCCGTAGCGTTTGGAA+AGG + Chr3:93038786-93038805 None:intergenic 50.0%
GAAGATGCCTTCTCTGTTGC+CGG - Chr3:93038847-93038866 MsG0380017178.01.T01:CDS 50.0%
GAAGGAGGATCATCAACGGT+CGG + Chr3:93039018-93039037 None:intergenic 50.0%
TAGCGTTTGGAAAGGACGGA+GGG + Chr3:93038778-93038797 None:intergenic 50.0%
TCCGTCCTTTCCAAACGCTA+CGG - Chr3:93038778-93038797 MsG0380017178.01.T01:CDS 50.0%
TGAATCCGCGAAGATCGAGA+CGG - Chr3:93039053-93039072 MsG0380017178.01.T01:CDS 50.0%
TGACGGGATTGAGATCCTTC+AGG - Chr3:93038894-93038913 MsG0380017178.01.T01:CDS 50.0%
TGATGATCCTCCTTCTGCAG+AGG - Chr3:93039023-93039042 MsG0380017178.01.T01:CDS 50.0%
!! GTTTGGTCGCCGTTTTGTTG+CGG + Chr3:93038664-93038683 None:intergenic 50.0%
AAAGGACGGAGGGAGTGGTT+AGG + Chr3:93038768-93038787 None:intergenic 55.0%
AAGGACGGAGGGAGTGGTTA+GGG + Chr3:93038767-93038786 None:intergenic 55.0%
ACGGCGCTAGAATCAACGGT+AGG + Chr3:93038970-93038989 None:intergenic 55.0%
AGTCTCCGTCTCGATCTTCG+CGG + Chr3:93039061-93039080 None:intergenic 55.0%
ATATGCTGAATCCGGCGGAG+GGG + Chr3:93038693-93038712 None:intergenic 55.0%
CATATGCTGAATCCGGCGGA+GGG + Chr3:93038694-93038713 None:intergenic 55.0%
GCCGTAGCGTTTGGAAAGGA+CGG + Chr3:93038782-93038801 None:intergenic 55.0%
GGGTGGCCATATGCTGAATC+CGG + Chr3:93038701-93038720 None:intergenic 55.0%
GTAGCGTTTGGAAAGGACGG+AGG + Chr3:93038779-93038798 None:intergenic 55.0%
GTTCCCCCACCGCAACAAAA+CGG - Chr3:93038652-93038671 MsG0380017178.01.T01:CDS 55.0%
TCAGCAGCATCCTCTGCAGA+AGG + Chr3:93039036-93039055 None:intergenic 55.0%
TGATAAGGACGCTGCTGCTG+TGG - Chr3:93038990-93039009 MsG0380017178.01.T01:CDS 55.0%
TGGCCATATGCTGAATCCGG+CGG + Chr3:93038698-93038717 None:intergenic 55.0%
TTCCTCGATCTGACGAGCAG+CGG + Chr3:93038830-93038849 None:intergenic 55.0%
TTTGGAAAGGACGGAGGGAG+TGG + Chr3:93038773-93038792 None:intergenic 55.0%
!! GTTTTGTTGCGGTGGGGGAA+CGG + Chr3:93038653-93038672 None:intergenic 55.0%
CCATATGCTGAATCCGGCGG+AGG + Chr3:93038695-93038714 None:intergenic 60.0%
CCTCCGCCGGATTCAGCATA+TGG - Chr3:93038692-93038711 MsG0380017178.01.T01:CDS 60.0%
GCAGCATCCTCTGCAGAAGG+AGG + Chr3:93039033-93039052 None:intergenic 60.0%
GGAGTCACCGGCAACAGAGA+AGG + Chr3:93038857-93038876 None:intergenic 60.0%
GGGCAATGTGCCGTAGCGTT+TGG + Chr3:93038791-93038810 None:intergenic 60.0%
TATGCTGAATCCGGCGGAGG+GGG + Chr3:93038692-93038711 None:intergenic 60.0%
!! GGTCGCCGTTTTGTTGCGGT+GGG + Chr3:93038660-93038679 None:intergenic 60.0%
!! GTCGCCGTTTTGTTGCGGTG+GGG + Chr3:93038659-93038678 None:intergenic 60.0%
!! TCGCCGTTTTGTTGCGGTGG+GGG + Chr3:93038658-93038677 None:intergenic 60.0%
!! TGGTCGCCGTTTTGTTGCGG+TGG + Chr3:93038661-93038680 None:intergenic 60.0%
ATGCTGAATCCGGCGGAGGG+GGG + Chr3:93038691-93038710 None:intergenic 65.0%
CAAACCAAACCCCCCTCCGC+CGG - Chr3:93038679-93038698 MsG0380017178.01.T01:CDS 65.0%
CTCCGCTGCTCGTCAGATCG+AGG - Chr3:93038825-93038844 MsG0380017178.01.T01:CDS 65.0%
CTCGATCTGACGAGCAGCGG+AGG + Chr3:93038827-93038846 None:intergenic 65.0%
GATCTGACGAGCAGCGGAGG+CGG + Chr3:93038824-93038843 None:intergenic 65.0%
TCAATCCCGTCACCACCGCC+AGG + Chr3:93038886-93038905 None:intergenic 65.0%
!! AGCGAGTGATGACGGCGTCG+CGG + Chr3:93038735-93038754 None:intergenic 65.0%
AGCAGCGGAGGCGGCTTCGT+CGG + Chr3:93038815-93038834 None:intergenic 70.0%
CCCGTCACCACCGCCAGGAA+CGG + Chr3:93038881-93038900 None:intergenic 70.0%
CCGTTCCTGGCGGTGGTGAC+GGG - Chr3:93038878-93038897 MsG0380017178.01.T01:CDS 70.0%
GAATCCGGCGGAGGGGGGTT+TGG + Chr3:93038686-93038705 None:intergenic 70.0%
GCCAGGAACGGCGGAGTCAC+CGG + Chr3:93038869-93038888 None:intergenic 70.0%
GCCGTTCCTGGCGGTGGTGA+CGG - Chr3:93038877-93038896 MsG0380017178.01.T01:CDS 70.0%
GGTGACTCCGCCGTTCCTGG+CGG - Chr3:93038868-93038887 MsG0380017178.01.T01:CDS 70.0%
GTCACCACCGCCAGGAACGG+CGG + Chr3:93038878-93038897 None:intergenic 70.0%
! CGGCGGAGGGGGGTTTGGTT+TGG + Chr3:93038681-93038700 None:intergenic 70.0%
!! GCGAGTGATGACGGCGTCGC+GGG + Chr3:93038734-93038753 None:intergenic 70.0%
AGCGGAGGCGGCTTCGTCGG+AGG + Chr3:93038812-93038831 None:intergenic 75.0%
GACGGCGTCGCGGGTGCGTT+GGG + Chr3:93038725-93038744 None:intergenic 75.0%
GACTCCGCCGTTCCTGGCGG+TGG - Chr3:93038871-93038890 MsG0380017178.01.T01:CDS 75.0%
GCCGGTGACTCCGCCGTTCC+TGG - Chr3:93038865-93038884 MsG0380017178.01.T01:CDS 75.0%
GCGGAGGCGGCTTCGTCGGA+GGG + Chr3:93038811-93038830 None:intergenic 75.0%
GCGTCGCGGGTGCGTTGGGT+GGG + Chr3:93038721-93038740 None:intergenic 75.0%
TCGCGGGTGCGTTGGGTGGG+TGG + Chr3:93038718-93038737 None:intergenic 75.0%
TGACGGCGTCGCGGGTGCGT+TGG + Chr3:93038726-93038745 None:intergenic 75.0%
! GGCGTCGCGGGTGCGTTGGG+TGG + Chr3:93038722-93038741 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr3 gene 93038604 93039089 93038604 ID=MsG0380017178.01;Name=MsG0380017178.01
Chr3 mRNA 93038604 93039089 93038604 ID=MsG0380017178.01.T01;Parent=MsG0380017178.01;Name=MsG0380017178.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|161
Chr3 exon 93038604 93039089 93038604 ID=MsG0380017178.01.T01:exon:5354;Parent=MsG0380017178.01.T01
Chr3 CDS 93038604 93039089 93038604 ID=MsG0380017178.01.T01:cds;Parent=MsG0380017178.01.T01
Gene Sequence

>MsG0380017178.01.T01

ATGTCCGACTCAGAAGTCACAACCGTAACCTCATCGGACTCTAACTCCGTTCCCCCACCGCAACAAAACGGCGACCAAACCAAACCCCCCTCCGCCGGATTCAGCATATGGCCACCCACCCAACGCACCCGCGACGCCGTCATCACTCGCTTAATCGAAACCCTAACCACTCCCTCCGTCCTTTCCAAACGCTACGGCACATTGCCCTCCGACGAAGCCGCCTCCGCTGCTCGTCAGATCGAGGAAGATGCCTTCTCTGTTGCCGGTGACTCCGCCGTTCCTGGCGGTGGTGACGGGATTGAGATCCTTCAGGTGTATTCCAAGGAAATCAGTAAGAAGATGCTTGAAACCGTTAAGGCTAGACCTACCGTTGATTCTAGCGCCGTTGATAAGGACGCTGCTGCTGTGGCACCGACCGTTGATGATCCTCCTTCTGCAGAGGATGCTGCTGAATCCGCGAAGATCGAGACGGAGACTGAAACCTGA

Protein sequence

>MsG0380017178.01.T01

MSDSEVTTVTSSDSNSVPPPQQNGDQTKPPSAGFSIWPPTQRTRDAVITRLIETLTTPSVLSKRYGTLPSDEAASAARQIEEDAFSVAGDSAVPGGGDGIEILQVYSKEISKKMLETVKARPTVDSSAVDKDAAAVAPTVDDPPSAEDAAESAKIETETET*