AlfalfaGEDB | MsG0380016669.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016669.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016669.01.T01	AFK46302.1	95.312	64	3	0	1	64	1	64	2.25E-12	69.7

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016669.01.T01	I3T1B0	95.312	64	3	0	1	64	1	64	1.08e-12	69.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049040.01	MsG0380016669.01	0.802389	5.741139e-49	2.808973e-46
MsG0080049068.01	MsG0380016669.01	0.822520	2.266825e-53	1.901902e-50
MsG0180000354.01	MsG0380016669.01	0.813920	2.004497e-51	1.326214e-48
MsG0180000355.01	MsG0380016669.01	0.820172	7.892059e-53	6.199106e-50
MsG0180000356.01	MsG0380016669.01	0.807205	5.663792e-50	3.135580e-47
MsG0180001085.01	MsG0380016669.01	0.827424	1.576437e-54	1.521372e-51
MsG0180001610.01	MsG0380016669.01	0.837253	5.796949e-57	7.486490e-54
MsG0180002024.01	MsG0380016669.01	0.801685	8.014285e-49	3.851100e-46
MsG0180002800.01	MsG0380016669.01	0.801109	1.051175e-48	4.978805e-46
MsG0180003353.01	MsG0380016669.01	0.845818	3.204175e-59	5.406291e-56
MsG0180004046.01	MsG0380016669.01	0.816842	4.490157e-52	3.217052e-49
MsG0180004428.01	MsG0380016669.01	0.805231	1.474875e-49	7.761448e-47
MsG0180004448.01	MsG0380016669.01	0.815999	6.933110e-52	4.854093e-49
MsG0180004466.01	MsG0380016669.01	0.863084	3.202758e-64	9.591521e-61
MsG0180004493.01	MsG0380016669.01	0.817224	3.684674e-52	2.667631e-49
MsG0180004564.01	MsG0380016669.01	0.801929	7.140022e-49	3.452438e-46
MsG0180004735.01	MsG0380016669.01	0.803660	3.135464e-49	1.584604e-46
MsG0180004759.01	MsG0380016669.01	-0.840477	8.492414e-58	1.211085e-54
MsG0180006031.01	MsG0380016669.01	0.821725	3.465533e-53	2.842240e-50
MsG0180006136.01	MsG0380016669.01	0.804833	1.786478e-49	9.306053e-47
MsG0380014907.01	MsG0380016669.01	0.824589	7.436737e-54	6.616644e-51
MsG0380014940.01	MsG0380016669.01	0.822092	2.850416e-53	2.362201e-50
MsG0380015352.01	MsG0380016669.01	0.846092	2.698706e-59	4.593182e-56
MsG0380015372.01	MsG0380016669.01	0.813330	2.703891e-51	1.760541e-48
MsG0380015743.01	MsG0380016669.01	0.800487	1.408350e-48	6.565858e-46
MsG0380015870.01	MsG0380016669.01	0.810552	1.088890e-50	6.583559e-48
MsG0380016540.01	MsG0380016669.01	0.840031	1.110407e-57	1.562107e-54
MsG0380016669.01	MsG0380016671.01	0.860348	2.199760e-63	5.994323e-60
MsG0380016669.01	MsG0380017659.01	0.814906	1.213510e-51	8.245555e-49
MsG0380016669.01	MsG0380017785.01	0.821312	4.317065e-53	3.499809e-50
MsG0380016669.01	MsG0380017968.01	0.841342	5.035144e-58	7.375931e-55
MsG0380016669.01	MsG0380018037.01	0.806409	8.342985e-50	4.524890e-47
MsG0380016669.01	MsG0380018061.01	0.810847	9.402530e-51	5.730126e-48
MsG0380016669.01	MsG0380018069.01	0.821810	3.312965e-53	2.723823e-50
MsG0380016669.01	MsG0480018577.01	0.841062	5.968309e-58	8.666479e-55
MsG0380016669.01	MsG0480018644.01	0.820288	7.423022e-53	5.849106e-50
MsG0380016669.01	MsG0480020690.01	0.817445	3.285395e-52	2.393149e-49
MsG0380016669.01	MsG0480020758.01	0.848047	7.862676e-60	1.424632e-56
MsG0380016669.01	MsG0480021803.01	0.800604	1.333174e-48	6.233773e-46
MsG0380016669.01	MsG0480022149.01	0.843117	1.706750e-58	2.643162e-55
MsG0380016669.01	MsG0480022260.01	0.828148	1.055868e-54	1.040708e-51
MsG0380016669.01	MsG0480022586.01	0.814947	1.188562e-51	8.085038e-49
MsG0380016669.01	MsG0480022587.01	0.801249	9.843711e-49	4.678688e-46
MsG0380016669.01	MsG0480023314.01	0.826072	3.316192e-54	3.077610e-51
MsG0380016669.01	MsG0580025366.01	0.810460	1.140095e-50	6.876163e-48
MsG0380016669.01	MsG0580025627.01	0.818173	2.250861e-52	1.672566e-49
MsG0380016669.01	MsG0580026175.01	0.806112	9.633862e-50	5.185730e-47
MsG0380016669.01	MsG0580026206.01	0.809398	1.929509e-50	1.131710e-47
MsG0380016669.01	MsG0580027394.01	0.829066	6.338549e-55	6.416228e-52
MsG0380016669.01	MsG0580028630.01	0.808605	2.852596e-50	1.638099e-47
MsG0380016669.01	MsG0680030313.01	0.803631	3.179852e-49	1.605858e-46
MsG0380016669.01	MsG0680030516.01	0.843253	1.569856e-58	2.441858e-55
MsG0380016669.01	MsG0680030627.01	0.823254	1.529136e-53	1.309760e-50
MsG0380016669.01	MsG0680030858.01	0.808428	3.111737e-50	1.778817e-47
MsG0380016669.01	MsG0680031939.01	0.815193	1.048036e-51	7.176880e-49
MsG0380016669.01	MsG0680033377.01	0.844180	8.869608e-59	1.420438e-55
MsG0380016669.01	MsG0680035731.01	0.818599	1.801978e-52	1.354801e-49
MsG0380016669.01	MsG0680035791.01	0.835167	1.964045e-56	2.382290e-53
MsG0380016669.01	MsG0680035859.01	0.810400	1.174558e-50	7.072693e-48
MsG0380016669.01	MsG0780036385.01	0.823473	1.359219e-53	1.171469e-50
MsG0380016669.01	MsG0780037395.01	0.804539	2.057701e-49	1.063696e-46
MsG0380016669.01	MsG0780038351.01	0.822973	1.778809e-53	1.511653e-50
MsG0380016669.01	MsG0780038616.01	0.819356	1.212417e-52	9.307873e-50
MsG0380016669.01	MsG0780038744.01	0.838032	3.659044e-57	4.840972e-54
MsG0380016669.01	MsG0780038784.01	0.809732	1.636039e-50	9.681397e-48
MsG0380016669.01	MsG0780039043.01	0.801128	1.041907e-48	4.937272e-46
MsG0380016669.01	MsG0780039746.01	0.804133	2.500330e-49	1.279108e-46
MsG0380016669.01	MsG0780039853.01	0.804583	2.015213e-49	1.042926e-46
MsG0380016669.01	MsG0780040388.01	0.815104	1.096563e-51	7.491021e-49
MsG0380016669.01	MsG0780040443.01	0.813488	2.495248e-51	1.631802e-48
MsG0380016669.01	MsG0780041138.01	0.839138	1.895143e-57	2.593030e-54
MsG0380016669.01	MsG0880041947.01	0.822457	2.345005e-53	1.963853e-50
MsG0380016669.01	MsG0880041954.01	0.842384	2.672492e-58	4.045554e-55
MsG0380016669.01	MsG0880042044.01	0.807522	4.850827e-50	2.707790e-47
MsG0380016669.01	MsG0880043277.01	0.839103	1.935447e-57	2.645381e-54
MsG0380016669.01	MsG0880044005.01	0.836441	9.342520e-57	1.177493e-53
MsG0380016669.01	MsG0880044142.01	0.856378	3.350064e-62	7.980440e-59
MsG0380016669.01	MsG0880044487.01	0.815713	8.030248e-52	5.578508e-49
MsG0380016669.01	MsG0880045101.01	0.822883	1.866681e-53	1.582291e-50
MsG0380016669.01	MsG0880045158.01	0.841309	5.139555e-58	7.520762e-55
MsG0380016669.01	MsG0880046054.01	0.837767	4.280879e-57	5.618141e-54
MsG0380016669.01	MsG0880046382.01	0.847544	1.081385e-59	1.928782e-56
MsG0380016669.01	MsG0880047754.01	0.806073	9.821352e-50	5.281287e-47
MsG0280006996.01	MsG0380016669.01	0.821354	4.222792e-53	3.427372e-50
MsG0280007309.01	MsG0380016669.01	0.801151	1.030626e-48	4.886625e-46
MsG0280007502.01	MsG0380016669.01	0.847722	9.662384e-60	1.733034e-56
MsG0280008388.01	MsG0380016669.01	0.807549	4.788768e-50	2.674864e-47
MsG0280008394.01	MsG0380016669.01	0.823648	1.237132e-53	1.071626e-50
MsG0280008451.01	MsG0380016669.01	0.850019	2.225640e-60	4.300238e-57
MsG0280008591.01	MsG0380016669.01	0.808361	3.216397e-50	1.835445e-47
MsG0280008942.01	MsG0380016669.01	0.824449	8.022130e-54	7.107410e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016669.01.T01	MTR_3g092980	95.312	64	3	0	1	64	1	64	3.20e-16	69.3
MsG0380016669.01.T01	MTR_3g092980	95.238	63	3	0	1	63	1	63	3.67e-15	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGTTATCATCCTCGACAAT+AGG	0.425509	3:-86417907	None:intergenic
CGTCGACGGAGGAAAGTTGT+TGG	0.457138	3:-86417760	None:intergenic
TTGTTGGTCGGTGGCTTCGT+CGG	0.466936	3:-86417744	None:intergenic
CTTGAGCGTTGTTTCGTCGA+CGG	0.472022	3:-86417774	None:intergenic
GTCGGCGGCAGCGTCAATGA+TGG	0.479255	3:-86417726	None:intergenic
CAAGAACAAACCACTTCCTC+AGG	0.481007	3:+86417793	MsG0380016669.01.T01:CDS
AAATGGAAAACCTGAGGAAG+TGG	0.520595	3:-86417803	None:intergenic
TCGGCGGCAGCGTCAATGAT+GGG	0.536510	3:-86417725	None:intergenic
GCAGCGTCAATGATGGGACC+TGG	0.558575	3:-86417719	None:intergenic
ATATCGTTAGCCATGTCTCC+AGG	0.560621	3:+86417701	None:intergenic
GGAGGAAAGTTGTTGGTCGG+TGG	0.573416	3:-86417753	None:intergenic
GAGCGTTGTTTCGTCGACGG+AGG	0.583352	3:-86417771	None:intergenic
TTGGTCGGTGGCTTCGTCGG+CGG	0.592563	3:-86417741	None:intergenic
GACGGAGGAAAGTTGTTGGT+CGG	0.601617	3:-86417756	None:intergenic
AATGATGGGACCTGGAGACA+TGG	0.605793	3:-86417711	None:intergenic
TACTATAAGATTCTACCTTG+AGG	0.646527	3:-86417973	None:intergenic
TGACGACAAAGACGAATCTG+AGG	0.650663	3:+86417930	MsG0380016669.01.T01:CDS
AGATGATGCTCCTATTGTCG+AGG	0.659014	3:+86417897	MsG0380016669.01.T01:CDS
GAAGACGACGAATCTCCTCA+AGG	0.684211	3:+86417958	MsG0380016669.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAAATAGAGAATAAATATA+TGG	-	Chr3:86417840-86417859	None:intergenic	15.0%
!!!	CATATATTTATTCTCTATTT+TGG	+	Chr3:86417838-86417857	MsG0380016669.01.T01:intron	15.0%
!	AATAAATATATGGAATCCGA+TGG	-	Chr3:86417830-86417849	None:intergenic	25.0%
!!!	CTGTTTTCTGTTTTTTCCTT+AGG	+	Chr3:86417870-86417889	MsG0380016669.01.T01:intron	30.0%
	ATATGGAATCCGATGGAAAA+TGG	-	Chr3:86417823-86417842	None:intergenic	35.0%
!	TCAGGTTTTCCATTTTCCAT+CGG	+	Chr3:86417811-86417830	MsG0380016669.01.T01:intron	35.0%
	AAATGGAAAACCTGAGGAAG+TGG	-	Chr3:86417806-86417825	None:intergenic	40.0%
	GATGGAAAATGGAAAACCTG+AGG	-	Chr3:86417812-86417831	None:intergenic	40.0%
	TCGTTATCATCCTCGACAAT+AGG	-	Chr3:86417910-86417929	None:intergenic	40.0%
	AGATGATGCTCCTATTGTCG+AGG	+	Chr3:86417897-86417916	MsG0380016669.01.T01:CDS	45.0%
	CAAGAACAAACCACTTCCTC+AGG	+	Chr3:86417793-86417812	MsG0380016669.01.T01:CDS	45.0%
	TGACGACAAAGACGAATCTG+AGG	+	Chr3:86417930-86417949	MsG0380016669.01.T01:CDS	45.0%
!	GGAGCATCATCTTCTTCCTA+AGG	-	Chr3:86417889-86417908	None:intergenic	45.0%
	GAAGACGACGAATCTCCTCA+AGG	+	Chr3:86417958-86417977	MsG0380016669.01.T01:CDS	50.0%
	GACGGAGGAAAGTTGTTGGT+CGG	-	Chr3:86417759-86417778	None:intergenic	50.0%
!	CTTGAGCGTTGTTTCGTCGA+CGG	-	Chr3:86417777-86417796	None:intergenic	50.0%
	CGTCGACGGAGGAAAGTTGT+TGG	-	Chr3:86417763-86417782	None:intergenic	55.0%
	GGAGGAAAGTTGTTGGTCGG+TGG	-	Chr3:86417756-86417775	None:intergenic	55.0%
	TTGTTGGTCGGTGGCTTCGT+CGG	-	Chr3:86417747-86417766	None:intergenic	55.0%
	TCGGCGGCAGCGTCAATGAT+GGG	-	Chr3:86417728-86417747	None:intergenic	60.0%
!	GAGCGTTGTTTCGTCGACGG+AGG	-	Chr3:86417774-86417793	None:intergenic	60.0%
!	GCAGCGTCAATGATGGGACC+TGG	-	Chr3:86417722-86417741	None:intergenic	60.0%
	GTCGGCGGCAGCGTCAATGA+TGG	-	Chr3:86417729-86417748	None:intergenic	65.0%
	TTGGTCGGTGGCTTCGTCGG+CGG	-	Chr3:86417744-86417763	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	86417713	86417993	86417713	ID=MsG0380016669.01;Name=MsG0380016669.01
Chr3	mRNA	86417713	86417993	86417713	ID=MsG0380016669.01.T01;Parent=MsG0380016669.01;Name=MsG0380016669.01.T01;_AED=0.50;_eAED=0.75;_QI=0\|0\|0\|1\|1\|1\|2\|0\|67
Chr3	exon	86417713	86417814	86417713	ID=MsG0380016669.01.T01:exon:22253;Parent=MsG0380016669.01.T01
Chr3	exon	86417892	86417993	86417892	ID=MsG0380016669.01.T01:exon:22254;Parent=MsG0380016669.01.T01
Chr3	CDS	86417713	86417814	86417713	ID=MsG0380016669.01.T01:cds;Parent=MsG0380016669.01.T01
Chr3	CDS	86417892	86417993	86417892	ID=MsG0380016669.01.T01:cds;Parent=MsG0380016669.01.T01

Gene Sequence	>MsG0380016669.01.T01 ATGTCTCCAGGTCCCATCATTGACGCTGCCGCCGACGAAGCCACCGACCAACAACTTTCCTCCGTCGACGAAACAACGCTCAAGAACAAACCACTTCCTCAGGAAGAAGATGATGCTCCTATTGTCGAGGATGATAACGATGACGACAAAGACGAATCTGAGGACGAAGAAGACGACGAATCTCCTCAAGGTAGAATCTTATAG
Protein sequence	>MsG0380016669.01.T01 MSPGPIIDAAADEATDQQLSSVDETTLKNKPLPQEEDDAPIVEDDNDDDKDESEDEEDDESPQGRIL*