AlfalfaGEDB | MsG0280007309.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007309.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007309.01.T01	XP_004494745.1	77.941	68	10	1	18	85	31	93	4.61E-26	107

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007309.01.T01	A0A1S2XWI5	77.941	68	10	1	18	85	31	93	2.20e-26	107

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048065.01	MsG0280007309.01	0.825132	5.538121e-54	5.004369e-51
MsG0080048727.01	MsG0280007309.01	0.827984	1.156522e-54	1.134503e-51
MsG0080048773.01	MsG0280007309.01	0.811306	7.479938e-51	4.613708e-48
MsG0080048826.01	MsG0280007309.01	0.808087	3.679589e-50	2.084552e-47
MsG0080048849.01	MsG0280007309.01	0.811877	5.620550e-51	3.519691e-48
MsG0080048878.01	MsG0280007309.01	0.814593	1.423612e-51	9.592483e-49
MsG0080049002.01	MsG0280007309.01	0.831849	1.322189e-55	1.452579e-52
MsG0080049068.01	MsG0280007309.01	0.829088	6.258046e-55	6.339478e-52
MsG0180000218.01	MsG0280007309.01	-0.803219	3.870098e-49	1.933887e-46
MsG0180000292.01	MsG0280007309.01	0.805481	1.307726e-49	6.926141e-47
MsG0180000354.01	MsG0280007309.01	0.832932	7.127283e-56	8.084553e-53
MsG0180000355.01	MsG0280007309.01	0.826035	3.383997e-54	3.137312e-51
MsG0180000534.01	MsG0280007309.01	0.803135	4.027676e-49	2.008268e-46
MsG0180000785.01	MsG0280007309.01	0.816051	6.747731e-52	4.731388e-49
MsG0180001064.01	MsG0280007309.01	0.808779	2.618767e-50	1.510668e-47
MsG0180001085.01	MsG0280007309.01	0.810115	1.353096e-50	8.087727e-48
MsG0180001105.01	MsG0280007309.01	0.807160	5.788415e-50	3.200805e-47
MsG0180001610.01	MsG0280007309.01	0.851978	6.240023e-61	1.284613e-57
MsG0180002024.01	MsG0280007309.01	0.821013	5.058295e-53	4.067349e-50
MsG0180002399.01	MsG0280007309.01	0.825845	3.754118e-54	3.461959e-51
MsG0180003353.01	MsG0280007309.01	0.859189	4.913344e-63	1.286503e-59
MsG0180003885.01	MsG0280007309.01	0.840840	6.822643e-58	9.840560e-55
MsG0180004062.01	MsG0280007309.01	0.809494	1.840441e-50	1.082170e-47
MsG0180004103.01	MsG0280007309.01	0.850828	1.319156e-60	2.617518e-57
MsG0180004192.01	MsG0280007309.01	0.825290	5.080125e-54	4.611212e-51
MsG0180004246.01	MsG0280007309.01	0.835475	1.641847e-56	2.009942e-53
MsG0180004428.01	MsG0280007309.01	0.840918	6.510707e-58	9.413191e-55
MsG0180004448.01	MsG0280007309.01	0.802744	4.852112e-49	2.395434e-46
MsG0180004466.01	MsG0280007309.01	0.867429	1.377491e-65	4.807814e-62
MsG0180004493.01	MsG0280007309.01	0.837015	6.670004e-57	8.553101e-54
MsG0180004500.01	MsG0280007309.01	0.802210	6.251565e-49	3.044483e-46
MsG0180004605.01	MsG0280007309.01	-0.826895	2.110052e-54	2.005033e-51
MsG0180004759.01	MsG0280007309.01	-0.817327	3.493791e-52	2.536591e-49
MsG0180005169.01	MsG0280007309.01	0.814904	1.214496e-51	8.251974e-49
MsG0180005807.01	MsG0280007309.01	0.828354	9.421501e-55	9.341344e-52
MsG0180006031.01	MsG0280007309.01	0.854028	1.616360e-61	3.562061e-58
MsG0180006096.01	MsG0280007309.01	0.807554	4.774940e-50	2.667574e-47
MsG0180006136.01	MsG0280007309.01	0.806125	9.576381e-50	5.156532e-47
MsG0280006309.01	MsG0280007309.01	0.801261	9.787579e-49	4.653407e-46
MsG0280006996.01	MsG0280007309.01	0.842846	2.014305e-58	3.093232e-55
MsG0280007075.01	MsG0280007309.01	0.801258	9.802284e-49	4.660027e-46
MsG0280007118.01	MsG0280007309.01	0.803412	3.530235e-49	1.772825e-46
MsG0280007307.01	MsG0280007309.01	0.845696	3.456544e-59	5.809007e-56
MsG0280007309.01	MsG0280007310.01	0.878367	2.975000e-69	1.549272e-65
MsG0280007309.01	MsG0280007432.01	0.829616	4.660391e-55	4.794602e-52
MsG0280007309.01	MsG0280007502.01	0.836259	1.039055e-56	1.302627e-53
MsG0280007309.01	MsG0280007647.01	0.804194	2.428431e-49	1.244249e-46
MsG0280007309.01	MsG0280007771.01	0.821427	4.061496e-53	3.303348e-50
MsG0280007309.01	MsG0280007814.01	0.818116	2.318585e-52	1.720192e-49
MsG0280007309.01	MsG0280007903.01	0.810686	1.018920e-50	6.182762e-48
MsG0280007309.01	MsG0280008451.01	0.815061	1.121336e-51	7.651399e-49
MsG0280007309.01	MsG0280008709.01	0.830897	2.267416e-55	2.421473e-52
MsG0280007309.01	MsG0280008942.01	0.834899	2.294495e-56	2.760718e-53
MsG0280007309.01	MsG0280009256.01	0.858017	1.099404e-62	2.766701e-59
MsG0280007309.01	MsG0280009691.01	0.805251	1.460806e-49	7.691095e-47
MsG0280007309.01	MsG0280009756.01	0.803316	3.694765e-49	1.850844e-46
MsG0280007309.01	MsG0280010353.01	0.804047	2.605341e-49	1.329914e-46
MsG0280007309.01	MsG0280010517.01	0.804134	2.498967e-49	1.278453e-46
MsG0280007309.01	MsG0280010564.01	0.810329	1.216562e-50	7.312105e-48
MsG0280007309.01	MsG0280010916.01	0.815833	7.549295e-52	5.261277e-49
MsG0280007309.01	MsG0280011027.01	0.826365	2.823232e-54	2.642219e-51
MsG0280007309.01	MsG0280011255.01	0.842710	2.189791e-58	3.348314e-55
MsG0280007309.01	MsG0380012005.01	0.802189	6.313472e-49	3.072972e-46
MsG0280007309.01	MsG0380012011.01	0.834685	2.598685e-56	3.106364e-53
MsG0280007309.01	MsG0380012012.01	0.816537	5.255164e-52	3.733742e-49
MsG0280007309.01	MsG0380013295.01	-0.830390	3.016419e-55	3.173704e-52
MsG0280007309.01	MsG0380014240.01	0.800935	1.140951e-48	5.380236e-46
MsG0280007309.01	MsG0380014383.01	0.821786	3.355476e-53	2.756837e-50
MsG0280007309.01	MsG0380014926.01	0.801265	9.768136e-49	4.644608e-46
MsG0280007309.01	MsG0380015352.01	0.800194	1.615892e-48	7.477435e-46
MsG0280007309.01	MsG0380015372.01	0.827383	1.613068e-54	1.554947e-51
MsG0280007309.01	MsG0380015603.01	0.813540	2.430363e-51	1.591594e-48
MsG0280007309.01	MsG0380015703.01	0.802102	6.578784e-49	3.195090e-46
MsG0280007309.01	MsG0380015784.01	0.844794	6.064294e-59	9.901515e-56
MsG0280007309.01	MsG0380015870.01	0.848139	7.412722e-60	1.347051e-56
MsG0280007309.01	MsG0380015885.01	0.803552	3.301312e-49	1.663925e-46
MsG0280007309.01	MsG0380015896.01	0.822479	2.317526e-53	1.942082e-50
MsG0280007309.01	MsG0380016036.01	0.802413	5.678351e-49	2.779814e-46
MsG0280007309.01	MsG0380016192.01	0.808287	3.335493e-50	1.899680e-47
MsG0280007309.01	MsG0380016294.01	0.850203	1.975960e-60	3.840800e-57
MsG0280007309.01	MsG0380016479.01	0.806889	6.605569e-50	3.627106e-47
MsG0280007309.01	MsG0380016540.01	0.844561	7.007509e-59	1.135786e-55
MsG0280007309.01	MsG0380016669.01	0.801151	1.030626e-48	4.886625e-46
MsG0280007309.01	MsG0380016671.01	0.818744	1.670226e-52	1.260828e-49
MsG0280007309.01	MsG0380016764.01	0.814944	1.190102e-51	8.094963e-49
MsG0280007309.01	MsG0380016820.01	0.834903	2.289471e-56	2.755029e-53
MsG0280007309.01	MsG0380016879.01	0.829085	6.269309e-55	6.350036e-52
MsG0280007309.01	MsG0380017202.01	0.819301	1.247767e-52	9.564735e-50
MsG0280007309.01	MsG0380017244.01	0.816870	4.423807e-52	3.172132e-49
MsG0280007309.01	MsG0380017293.01	0.801991	6.933279e-49	3.357716e-46
MsG0280007309.01	MsG0380017931.01	0.837099	6.348148e-57	8.160495e-54
MsG0280007309.01	MsG0380018037.01	0.836318	1.003909e-56	1.260793e-53
MsG0280007309.01	MsG0380018061.01	0.819110	1.379083e-52	1.051646e-49
MsG0280007309.01	MsG0380018069.01	0.829053	6.384127e-55	6.459720e-52
MsG0280007309.01	MsG0480018584.01	0.867233	1.591249e-65	5.515433e-62
MsG0280007309.01	MsG0480018653.01	0.817595	3.039506e-52	2.223039e-49
MsG0280007309.01	MsG0480018911.01	0.824415	8.172374e-54	7.233443e-51
MsG0280007309.01	MsG0480019007.01	0.823539	1.311929e-53	1.132796e-50
MsG0280007309.01	MsG0480019711.01	0.808393	3.165917e-50	1.808132e-47
MsG0280007309.01	MsG0480020024.01	0.836946	6.946484e-57	8.888993e-54
MsG0280007309.01	MsG0480020690.01	0.824137	9.501813e-54	8.344305e-51
MsG0280007309.01	MsG0480020889.01	0.807013	6.217712e-50	3.425137e-47
MsG0280007309.01	MsG0480020935.01	-0.813572	2.392211e-51	1.567892e-48
MsG0280007309.01	MsG0480021060.01	0.815753	7.864716e-52	5.469725e-49
MsG0280007309.01	MsG0480021248.01	0.821254	4.452017e-53	3.603340e-50
MsG0280007309.01	MsG0480022149.01	0.820552	6.457012e-53	5.125287e-50
MsG0280007309.01	MsG0480022200.01	0.806207	9.199371e-50	4.964186e-47
MsG0280007309.01	MsG0480022260.01	0.841263	5.281758e-58	7.718400e-55
MsG0280007309.01	MsG0480022355.01	0.801910	7.206080e-49	3.482749e-46
MsG0280007309.01	MsG0480022491.01	-0.824945	6.128990e-54	5.508935e-51
MsG0280007309.01	MsG0480022586.01	0.811413	7.087414e-51	4.384392e-48
MsG0280007309.01	MsG0480022624.01	0.860823	1.578531e-63	4.372550e-60
MsG0280007309.01	MsG0480022994.01	0.841580	4.360294e-58	6.435329e-55
MsG0280007309.01	MsG0480023073.01	0.825856	3.732086e-54	3.442748e-51
MsG0280007309.01	MsG0480023190.01	0.804572	2.025679e-49	1.048051e-46
MsG0280007309.01	MsG0480023384.01	0.809264	2.062464e-50	1.205327e-47
MsG0280007309.01	MsG0480023565.01	0.808671	2.761330e-50	1.588470e-47
MsG0280007309.01	MsG0480023845.01	0.811450	6.959815e-51	4.309603e-48
MsG0280007309.01	MsG0480024030.01	0.810487	1.124644e-50	6.788108e-48
MsG0280007309.01	MsG0580024115.01	0.815810	7.640186e-52	5.321561e-49
MsG0280007309.01	MsG0580024790.01	-0.830545	2.764335e-55	2.921480e-52
MsG0280007309.01	MsG0580024997.01	0.810240	1.271869e-50	7.626771e-48
MsG0280007309.01	MsG0580025059.01	0.822817	1.933747e-53	1.636106e-50
MsG0280007309.01	MsG0580025407.01	-0.804333	2.272316e-49	1.168423e-46
MsG0280007309.01	MsG0580025502.01	0.808754	2.651610e-50	1.528646e-47
MsG0280007309.01	MsG0580025588.01	0.807272	5.480337e-50	3.039402e-47
MsG0280007309.01	MsG0580025816.01	0.802297	5.998829e-49	2.928091e-46
MsG0280007309.01	MsG0580025845.01	0.823473	1.359312e-53	1.171540e-50
MsG0280007309.01	MsG0580025859.01	0.820545	6.482721e-53	5.144642e-50
MsG0280007309.01	MsG0580026175.01	0.842551	2.414052e-58	3.673284e-55
MsG0280007309.01	MsG0580027394.01	0.850057	2.172231e-60	4.202574e-57
MsG0280007309.01	MsG0580027763.01	0.804495	2.101942e-49	1.085322e-46
MsG0280007309.01	MsG0580027928.01	-0.801151	1.030932e-48	4.888014e-46
MsG0280007309.01	MsG0580028033.01	0.817990	2.474941e-52	1.829885e-49
MsG0280007309.01	MsG0580028059.01	0.804544	2.053446e-49	1.061614e-46
MsG0280007309.01	MsG0580028551.01	0.807071	6.044695e-50	3.334778e-47
MsG0280007309.01	MsG0580028570.01	0.802617	5.154063e-49	2.536244e-46
MsG0280007309.01	MsG0580028598.01	0.802447	5.587449e-49	2.737617e-46
MsG0280007309.01	MsG0580028630.01	0.819036	1.434183e-52	1.091472e-49
MsG0280007309.01	MsG0580029236.01	0.808951	2.405941e-50	1.394278e-47
MsG0280007309.01	MsG0580029529.01	0.810733	9.953706e-51	6.047544e-48
MsG0280007309.01	MsG0580029862.01	0.803683	3.101379e-49	1.568322e-46
MsG0280007309.01	MsG0580029879.01	0.800640	1.310904e-48	6.135318e-46
MsG0280007309.01	MsG0680030313.01	0.867665	1.157214e-65	4.072927e-62
MsG0280007309.01	MsG0680030373.01	0.852714	3.850555e-61	8.118123e-58
MsG0280007309.01	MsG0680030514.01	0.803458	3.452544e-49	1.735900e-46
MsG0280007309.01	MsG0680030515.01	0.824542	7.627412e-54	6.776634e-51
MsG0280007309.01	MsG0680030516.01	0.837401	5.313098e-57	6.893190e-54
MsG0280007309.01	MsG0680030599.01	0.809896	1.508420e-50	8.964126e-48
MsG0280007309.01	MsG0680030858.01	0.841611	4.278807e-58	6.321528e-55
MsG0280007309.01	MsG0680031146.01	0.813861	2.065628e-51	1.364485e-48
MsG0280007309.01	MsG0680031640.01	0.804662	1.939853e-49	1.005991e-46
MsG0280007309.01	MsG0680031939.01	0.806844	6.753883e-50	3.704302e-47
MsG0280007309.01	MsG0680032827.01	0.820597	6.305972e-53	5.011173e-50
MsG0280007309.01	MsG0680032830.01	0.835460	1.656376e-56	2.026855e-53
MsG0280007309.01	MsG0680032832.01	0.802423	5.649316e-49	2.766404e-46
MsG0280007309.01	MsG0680033377.01	0.848308	6.659239e-60	1.216968e-56
MsG0280007309.01	MsG0680033465.01	0.801573	8.448528e-49	4.048457e-46
MsG0280007309.01	MsG0680034766.01	0.806930	6.476642e-50	3.560051e-47
MsG0280007309.01	MsG0680034838.01	0.810526	1.103234e-50	6.665566e-48
MsG0280007309.01	MsG0680035731.01	0.809578	1.765205e-50	1.040331e-47
MsG0280007309.01	MsG0680035791.01	0.806578	7.686729e-50	4.186944e-47
MsG0280007309.01	MsG0680035859.01	0.843929	1.035222e-58	1.645017e-55
MsG0280007309.01	MsG0780036307.01	0.801611	8.298160e-49	3.980094e-46
MsG0280007309.01	MsG0780036385.01	0.809763	1.611312e-50	9.543009e-48
MsG0280007309.01	MsG0780036517.01	0.850536	1.593843e-60	3.131876e-57
MsG0280007309.01	MsG0780036593.01	0.803727	3.037162e-49	1.537603e-46
MsG0280007309.01	MsG0780036659.01	0.835512	1.607463e-56	1.970020e-53
MsG0280007309.01	MsG0780036660.01	0.822734	2.021890e-53	1.706704e-50
MsG0280007309.01	MsG0780036757.01	0.823016	1.737545e-53	1.478470e-50
MsG0280007309.01	MsG0780037093.01	0.801378	9.261504e-49	4.416268e-46
MsG0280007309.01	MsG0780037157.01	0.805845	1.096552e-49	5.862483e-47
MsG0280007309.01	MsG0780037281.01	0.817045	4.042336e-52	2.912412e-49
MsG0280007309.01	MsG0780037341.01	0.813529	2.444758e-51	1.600545e-48
MsG0280007309.01	MsG0780037395.01	0.838518	2.742422e-57	3.682161e-54
MsG0280007309.01	MsG0780037701.01	0.804402	2.198020e-49	1.132213e-46
MsG0280007309.01	MsG0780038378.01	0.831138	1.978420e-55	2.127840e-52
MsG0280007309.01	MsG0780038616.01	0.812919	3.327205e-51	2.142385e-48
MsG0280007309.01	MsG0780038744.01	0.811151	8.078925e-51	4.963225e-48
MsG0280007309.01	MsG0780038784.01	0.811334	7.374954e-51	4.552340e-48
MsG0280007309.01	MsG0780039155.01	0.827596	1.433584e-54	1.390204e-51
MsG0280007309.01	MsG0780039186.01	0.811076	8.387382e-51	5.142565e-48
MsG0280007309.01	MsG0780039429.01	0.815405	9.400931e-52	6.475642e-49
MsG0280007309.01	MsG0780039439.01	0.802166	6.383503e-49	3.105324e-46
MsG0280007309.01	MsG0780039853.01	0.813108	3.024455e-51	1.957272e-48
MsG0280007309.01	MsG0780040388.01	0.855203	7.386159e-62	1.691766e-58
MsG0280007309.01	MsG0780040763.01	0.812586	3.934398e-51	2.511372e-48
MsG0280007309.01	MsG0780041091.01	0.832796	7.704277e-56	8.703507e-53
MsG0280007309.01	MsG0780041138.01	0.848332	6.557863e-60	1.199322e-56
MsG0280007309.01	MsG0780041634.01	0.857885	1.203496e-62	3.015048e-59
MsG0280007309.01	MsG0780041673.01	0.818183	2.239273e-52	1.664432e-49
MsG0280007309.01	MsG0780041747.01	0.804614	1.984888e-49	1.028065e-46
MsG0280007309.01	MsG0880041861.01	0.806758	7.041090e-50	3.853079e-47
MsG0280007309.01	MsG0880041947.01	0.840790	7.033411e-58	1.012865e-54
MsG0280007309.01	MsG0880041954.01	0.806476	8.074935e-50	4.387161e-47
MsG0280007309.01	MsG0880042022.01	0.835385	1.730870e-56	2.113306e-53
MsG0280007309.01	MsG0880043157.01	0.828036	1.123880e-54	1.104133e-51
MsG0280007309.01	MsG0880043277.01	0.834971	2.200880e-56	2.654074e-53
MsG0280007309.01	MsG0880043955.01	0.813028	3.149649e-51	2.034019e-48
MsG0280007309.01	MsG0880043965.01	0.812051	5.150680e-51	3.240641e-48
MsG0280007309.01	MsG0880044005.01	0.837416	5.266383e-57	6.835960e-54
MsG0280007309.01	MsG0880044036.01	0.819170	1.336911e-52	1.021129e-49
MsG0280007309.01	MsG0880044142.01	0.830008	3.739906e-55	3.891539e-52
MsG0280007309.01	MsG0880044392.01	0.823405	1.410016e-53	1.212807e-50
MsG0280007309.01	MsG0880044487.01	0.833370	5.544037e-56	6.372078e-53
MsG0280007309.01	MsG0880044691.01	0.813414	2.590755e-51	1.690832e-48
MsG0280007309.01	MsG0880045158.01	0.840134	1.043887e-57	1.473106e-54
MsG0280007309.01	MsG0880045668.01	0.813562	2.403768e-51	1.575029e-48
MsG0280007309.01	MsG0880045722.01	0.809663	1.692988e-50	1.000007e-47
MsG0280007309.01	MsG0880045992.01	0.829754	4.311866e-55	4.454576e-52
MsG0280007309.01	MsG0880046054.01	0.806006	1.014312e-49	5.445372e-47
MsG0280007309.01	MsG0880046382.01	0.820111	8.149305e-53	6.391074e-50
MsG0280007309.01	MsG0880046765.01	0.812145	4.912756e-51	3.099069e-48
MsG0280007309.01	MsG0880046867.01	0.802164	6.388470e-49	3.107618e-46
MsG0280007309.01	MsG0880047394.01	0.841319	5.106023e-58	7.474157e-55
MsG0280007309.01	MsG0880047422.01	-0.816565	5.179639e-52	3.682923e-49
MsG0280007309.01	MsG0880047481.01	-0.802622	5.141742e-49	2.530475e-46
MsG0280007309.01	MsG0880047538.01	0.846592	1.971615e-59	3.410597e-56
MsG0280007309.01	MsG0880047696.01	0.810364	1.195873e-50	7.194450e-48
MsG0280007309.01	MsG0880047703.01	0.832683	8.218186e-56	9.253071e-53
MsG0280007309.01	MsG0880047754.01	0.814269	1.678728e-51	1.121300e-48
MsG0280007309.01	MsG0880047770.01	0.835666	1.469296e-56	1.808977e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007309.01.T01	MTR_7g115670	85.507	69	7	1	18	86	34	99	3.67e-28	102

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007309.01.T01	AT1G08640	45.833	72	34	1	14	85	41	107	3.80e-13	63.2

Find 16 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAAGCAGAGATGATTAT+TGG	0.153927	2:-13358553	None:intergenic
AAAGAAGGAAGCTGAGATTA+AGG	0.221315	2:+13358790	MsG0280007309.01.T01:CDS
ATCGTGTCAAATTTCTCAAT+AGG	0.342314	2:-13358746	None:intergenic
TCGTGTCAAATTTCTCAATA+GGG	0.363102	2:-13358745	None:intergenic
AAGAAGCAGAGATGATTATT+GGG	0.389670	2:-13358552	None:intergenic
TCTTCTTCTGCATCTGCTGC+AGG	0.410429	2:+13358580	MsG0280007309.01.T01:CDS
ATTAGTTAGTTAACATACTC+TGG	0.448720	2:-13358830	None:intergenic
AAGAAGGAAGCTGAGATTAA+GGG	0.453722	2:+13358791	MsG0280007309.01.T01:CDS
TATTGAGTAGCTACCTCATA+AGG	0.456840	2:-13358631	None:intergenic
CGGTGACTTCAATCCTTATG+AGG	0.484013	2:+13358618	MsG0280007309.01.T01:CDS
AGGAACAAGAAGTGTTGGAG+TGG	0.521969	2:+13358256	MsG0280007309.01.T01:CDS
TAAGGATTGAAGTCACCGTT+AGG	0.528101	2:-13358613	None:intergenic
TGTTCAGGAACAAGAAGTGT+TGG	0.539296	2:+13358251	MsG0280007309.01.T01:CDS
GGTTTCCCAAGAAAACATCA+TGG	0.552983	2:+13358524	MsG0280007309.01.T01:intron
GGAACAAGAAGTGTTGGAGT+GGG	0.628937	2:+13358257	MsG0280007309.01.T01:CDS
GCAGGAAGCACTAATCCTAA+CGG	0.643511	2:+13358598	MsG0280007309.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTAATATGATAGAATAATA+AGG	-	Chr2:13358686-13358705	None:intergenic	15.0%
!!!	AAAAAAAAACATTTTCTTAC+TGG	+	Chr2:13358484-13358503	MsG0280007309.01.T01:intron	15.0%
!!!	AAAAAAAACATTTTCTTACT+GGG	+	Chr2:13358485-13358504	MsG0280007309.01.T01:intron	15.0%
!!!	ATTATTGTTGTATAGATTTT+AGG	+	Chr2:13358716-13358735	MsG0280007309.01.T01:intron	15.0%
!!	AAATTCAATTCAAAGAGTTT+AGG	-	Chr2:13358329-13358348	None:intergenic	20.0%
!!	CTTTGAATTGAATTTGAAAA+TGG	+	Chr2:13358334-13358353	MsG0280007309.01.T01:intron	20.0%
!!!	TGTTTTTTTTTAGAAGAAGA+AGG	-	Chr2:13358475-13358494	None:intergenic	20.0%
!!	TGTTGTTGTTGTTGAATTTA+GGG	-	Chr2:13358387-13358406	None:intergenic	25.0%
!!	TTATTCCATGATGTTTTCTT+GGG	-	Chr2:13358532-13358551	None:intergenic	25.0%
!!	TTGTTGTTGTTGTTGAATTT+AGG	-	Chr2:13358388-13358407	None:intergenic	25.0%
	AAGAAGCAGAGATGATTATT+GGG	-	Chr2:13358555-13358574	None:intergenic	30.0%
	AGCATATACCAAGAAAAAGA+AGG	+	Chr2:13358775-13358794	MsG0280007309.01.T01:CDS	30.0%
	ATCGTGTCAAATTTCTCAAT+AGG	-	Chr2:13358749-13358768	None:intergenic	30.0%
	TCGTGTCAAATTTCTCAATA+GGG	-	Chr2:13358748-13358767	None:intergenic	30.0%
!	AAACATTTTCTTACTGGGTT+TGG	+	Chr2:13358490-13358509	MsG0280007309.01.T01:intron	30.0%
!	GTTATTCCATGATGTTTTCT+TGG	-	Chr2:13358533-13358552	None:intergenic	30.0%
	AAAGAAGGAAGCTGAGATTA+AGG	+	Chr2:13358790-13358809	MsG0280007309.01.T01:CDS	35.0%
	AAATTGGGAACGGAAATAGT+TGG	-	Chr2:13358422-13358441	None:intergenic	35.0%
	AAGAAGGAAGCTGAGATTAA+GGG	+	Chr2:13358791-13358810	MsG0280007309.01.T01:CDS	35.0%
	GAAGAAGCAGAGATGATTAT+TGG	-	Chr2:13358556-13358575	None:intergenic	35.0%
	TATTGAGTAGCTACCTCATA+AGG	-	Chr2:13358634-13358653	None:intergenic	35.0%
	ACCTGAGAGAGGTTGAAAAT+TGG	-	Chr2:13358438-13358457	None:intergenic	40.0%
	CCTGAGAGAGGTTGAAAATT+GGG	-	Chr2:13358437-13358456	None:intergenic	40.0%
	GAGAGGTTGAAAATTGGGAA+CGG	-	Chr2:13358432-13358451	None:intergenic	40.0%
	GGTTTCCCAAGAAAACATCA+TGG	+	Chr2:13358524-13358543	MsG0280007309.01.T01:intron	40.0%
	TAAGGATTGAAGTCACCGTT+AGG	-	Chr2:13358616-13358635	None:intergenic	40.0%
!	CTCAGCTTCCTTCTTTTTCT+TGG	-	Chr2:13358786-13358805	None:intergenic	40.0%
!	TGTTCAGGAACAAGAAGTGT+TGG	+	Chr2:13358251-13358270	MsG0280007309.01.T01:CDS	40.0%
!!	CTGGGTTTGGTTCAATTTTC+AGG	+	Chr2:13358503-13358522	MsG0280007309.01.T01:intron	40.0%
	CGGTGACTTCAATCCTTATG+AGG	+	Chr2:13358618-13358637	MsG0280007309.01.T01:CDS	45.0%
!	CCCAATTTTCAACCTCTCTC+AGG	+	Chr2:13358434-13358453	MsG0280007309.01.T01:intron	45.0%
!!	AGGAACAAGAAGTGTTGGAG+TGG	+	Chr2:13358256-13358275	MsG0280007309.01.T01:CDS	45.0%
!!	GCAGGAAGCACTAATCCTAA+CGG	+	Chr2:13358598-13358617	MsG0280007309.01.T01:CDS	45.0%
!!	GGAACAAGAAGTGTTGGAGT+GGG	+	Chr2:13358257-13358276	MsG0280007309.01.T01:CDS	45.0%
	GAGTGAGTGTTACCTGAGAG+AGG	-	Chr2:13358449-13358468	None:intergenic	50.0%
	TCTTCTTCTGCATCTGCTGC+AGG	+	Chr2:13358580-13358599	MsG0280007309.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	13358246	13358847	13358246	ID=MsG0280007309.01;Name=MsG0280007309.01
Chr2	mRNA	13358246	13358847	13358246	ID=MsG0280007309.01.T01;Parent=MsG0280007309.01;Name=MsG0280007309.01.T01;_AED=0.48;_eAED=0.60;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|0\|93
Chr2	exon	13358246	13358295	13358246	ID=MsG0280007309.01.T01:exon:2508;Parent=MsG0280007309.01.T01
Chr2	exon	13358525	13358639	13358525	ID=MsG0280007309.01.T01:exon:2509;Parent=MsG0280007309.01.T01
Chr2	exon	13358731	13358847	13358731	ID=MsG0280007309.01.T01:exon:2510;Parent=MsG0280007309.01.T01
Chr2	CDS	13358246	13358295	13358246	ID=MsG0280007309.01.T01:cds;Parent=MsG0280007309.01.T01
Chr2	CDS	13358525	13358639	13358525	ID=MsG0280007309.01.T01:cds;Parent=MsG0280007309.01.T01
Chr2	CDS	13358731	13358847	13358731	ID=MsG0280007309.01.T01:cds;Parent=MsG0280007309.01.T01

Gene Sequence	>MsG0280007309.01.T01 ATGTATGTTCAGGAACAAGAAGTGTTGGAGTGGGAAATTGCAGTTGTGTTGTTTCCCAAGAAAACATCATGGAATAACCCAATAATCATCTCTGCTTCTTCTTCTTCTTCTTCTGCATCTGCTGCAGGAAGCACTAATCCTAACGGTGACTTCAATCCTTATGAGATTTTAGGTGTAAGCCCTATTGAGAAATTTGACACGATCAAAGCAGCATATACCAAGAAAAAGAAGGAAGCTGAGATTAAGGGTGATGAACAACTGCTTCCAGAGTATGTTAACTAA
Protein sequence	>MsG0280007309.01.T01 MYVQEQEVLEWEIAVVLFPKKTSWNNPIIISASSSSSSASAAGSTNPNGDFNPYEILGVSPIEKFDTIKAAYTKKKKEAEIKGDEQLLPEYVN*