AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023163.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023163.01.T01 MTR_8g088380 98.400 125 2 0 5 129 3 127 1.60e-87 250
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023163.01.T01 AT3G29130 64.103 117 42 0 13 129 3 119 3.34e-55 168
MsG0480023163.01.T01 AT3G29130 64.103 117 42 0 13 129 3 119 3.34e-55 168
MsG0480023163.01.T01 AT3G29130 64.103 117 42 0 13 129 3 119 3.34e-55 168
MsG0480023163.01.T01 AT3G29130 64.103 117 42 0 13 129 52 168 1.02e-54 169
MsG0480023163.01.T01 AT3G29130 65.000 80 28 0 50 129 12 91 1.04e-36 120
MsG0480023163.01.T01 AT3G29130 65.000 80 28 0 50 129 12 91 1.04e-36 120

Find 16 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATAAGTTGTTGAAAGTT+TGG 0.311208 4:-81630732 None:intergenic
GAATCAGAATCGATGAAATT+AGG 0.315765 4:+81628734 MsG0480023163.01.T01:CDS
TAGTGTGTTGTAAATGGTTA+AGG 0.342554 4:-81628814 None:intergenic
TTTCAGATTGGGAAGGTTGC+AGG 0.352781 4:+81628918 MsG0480023163.01.T01:intron
CATACATAGTGTGTTGTAAA+TGG 0.357309 4:-81628820 None:intergenic
TACTTAGGCATTTCAAGATC+CGG 0.393355 4:-81630800 None:intergenic
GAATCTTCAGGAAATGCATC+TGG 0.446985 4:-81630752 None:intergenic
GATAACAGCAATAGTTACTT+AGG 0.475331 4:-81630815 None:intergenic
ACGTTGCTCATAGAATCTTC+AGG 0.493306 4:-81630764 None:intergenic
AGTGTGTTGTAAATGGTTAA+GGG 0.496258 4:-81628813 None:intergenic
CTATGAGCAACGTTATTGAC+AGG 0.505789 4:+81630774 MsG0480023163.01.T01:CDS
CGTGTGTTTCTAGCCATGTC+AGG 0.515825 4:-81629003 None:intergenic
TACTCAGATTTCTCCTGACA+TGG 0.531893 4:+81628990 MsG0480023163.01.T01:CDS
CAACGTTATTGACAGGAGAC+CGG 0.580638 4:+81630781 MsG0480023163.01.T01:CDS
AATTGAATTTCAGATTGGGA+AGG 0.586674 4:+81628911 MsG0480023163.01.T01:intron
GTTAAGGGAACGAAGATCAT+CGG 0.608518 4:-81628798 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACATCAAATTATATAAT+TGG + Chr4:81629119-81629138 MsG0480023163.01.T01:intron 10.0%
!! GAATATATTGATATTAATTT+GGG + Chr4:81629194-81629213 MsG0480023163.01.T01:intron 10.0%
!! TGAATATATTGATATTAATT+TGG + Chr4:81629193-81629212 MsG0480023163.01.T01:intron 10.0%
!!! AATATATTGATATTAATTTG+GGG + Chr4:81629195-81629214 MsG0480023163.01.T01:intron 10.0%
!!! ATTATATTTTTCTTAAACTT+AGG + Chr4:81629055-81629074 MsG0480023163.01.T01:CDS 10.0%
!!! TTATTGAAAATTCTTAATTT+TGG + Chr4:81629482-81629501 MsG0480023163.01.T01:intron 10.0%
!!! AATTTTCAATAAATAGAATC+AGG - Chr4:81629474-81629493 None:intergenic 15.0%
!!! ATATATTGATATTAATTTGG+GGG + Chr4:81629196-81629215 MsG0480023163.01.T01:intron 15.0%
!!! ATTAAATCTTATGATTTTCT+TGG + Chr4:81629603-81629622 MsG0480023163.01.T01:intron 15.0%
!! AATTGAATTGAATTTCAGAT+TGG + Chr4:81628906-81628925 MsG0480023163.01.T01:intron 20.0%
!! ATCATACTTACTAACTAAAA+GGG + Chr4:81629877-81629896 MsG0480023163.01.T01:intron 20.0%
!! ATTGAATTGAATTTCAGATT+GGG + Chr4:81628907-81628926 MsG0480023163.01.T01:intron 20.0%
!! CAAAAAAAATAAGCAATACA+AGG + Chr4:81629170-81629189 MsG0480023163.01.T01:intron 20.0%
!! CATAAATCTCAATAAGTTAA+AGG - Chr4:81629819-81629838 None:intergenic 20.0%
!! TCTTGTAAACTAACAAAATT+TGG + Chr4:81629961-81629980 MsG0480023163.01.T01:intron 20.0%
!! TTAATAAAAAAAGAGAGAAG+AGG - Chr4:81628852-81628871 None:intergenic 20.0%
!! TTTACAATTGGATAATAACA+AGG + Chr4:81630295-81630314 MsG0480023163.01.T01:intron 20.0%
!!! ATGTTTAGACAATATAGTTT+TGG + Chr4:81629914-81629933 MsG0480023163.01.T01:intron 20.0%
!!! TAAATAGAATCAGGTATTTT+TGG - Chr4:81629465-81629484 None:intergenic 20.0%
!!! TTTTAGTTAGTAAGTATGAT+GGG - Chr4:81629877-81629896 None:intergenic 20.0%
! ACTCGTATTTCTTTGATTAT+GGG - Chr4:81629648-81629667 None:intergenic 25.0%
! CATCATACTTACTAACTAAA+AGG + Chr4:81629876-81629895 MsG0480023163.01.T01:intron 25.0%
! GATAAACATAACCAAATTTG+AGG - Chr4:81629554-81629573 None:intergenic 25.0%
! TCATACTTACTAACTAAAAG+GGG + Chr4:81629878-81629897 MsG0480023163.01.T01:intron 25.0%
!! AAAAGTTGCTGAAATTTTTC+TGG - Chr4:81629735-81629754 None:intergenic 25.0%
!! AAATAAGTTCAAGTTACCTA+AGG - Chr4:81630437-81630456 None:intergenic 25.0%
!! TATTCTGAGAGAATACAATT+CGG - Chr4:81629371-81629390 None:intergenic 25.0%
!! TTTTACTAATGTAACCTAGA+TGG + Chr4:81630461-81630480 MsG0480023163.01.T01:intron 25.0%
!!! CTTTTAGTTAGTAAGTATGA+TGG - Chr4:81629878-81629897 None:intergenic 25.0%
AAATGCCATTGTTACTGTAA+TGG - Chr4:81630198-81630217 None:intergenic 30.0%
AATTGAATTTCAGATTGGGA+AGG + Chr4:81628911-81628930 MsG0480023163.01.T01:intron 30.0%
ACTTCTTATTAAACCAAGAG+TGG + Chr4:81630370-81630389 MsG0480023163.01.T01:intron 30.0%
AGATGAACAGTAGAAGAAAA+AGG + Chr4:81630561-81630580 MsG0480023163.01.T01:intron 30.0%
AGTGTGTTGTAAATGGTTAA+GGG - Chr4:81628816-81628835 None:intergenic 30.0%
AGTTGAAAAACATATGCTGA+TGG + Chr4:81630527-81630546 MsG0480023163.01.T01:intron 30.0%
ATAAGCTTACATTCAAGAGT+TGG + Chr4:81629406-81629425 MsG0480023163.01.T01:intron 30.0%
ATCTCAGACATTAATTCCTT+AGG + Chr4:81630418-81630437 MsG0480023163.01.T01:intron 30.0%
ATTACAGTAACAATGGCATT+TGG + Chr4:81630197-81630216 MsG0480023163.01.T01:intron 30.0%
ATTTATGTTCTCGCTAAGTT+TGG + Chr4:81629832-81629851 MsG0480023163.01.T01:intron 30.0%
CATACATAGTGTGTTGTAAA+TGG - Chr4:81628823-81628842 None:intergenic 30.0%
CATCTATATCACCTCAAATT+TGG + Chr4:81629540-81629559 MsG0480023163.01.T01:intron 30.0%
CTAACAGAGATAGAAATAGA+AGG - Chr4:81630629-81630648 None:intergenic 30.0%
CTTCTTATTAAACCAAGAGT+GGG + Chr4:81630371-81630390 MsG0480023163.01.T01:intron 30.0%
GAATCAGAATCGATGAAATT+AGG + Chr4:81628734-81628753 MsG0480023163.01.T01:CDS 30.0%
GACTCGTATTTCTTTGATTA+TGG - Chr4:81629649-81629668 None:intergenic 30.0%
GATGAACAGTAGAAGAAAAA+GGG + Chr4:81630562-81630581 MsG0480023163.01.T01:intron 30.0%
TAAGCTTACATTCAAGAGTT+GGG + Chr4:81629407-81629426 MsG0480023163.01.T01:intron 30.0%
TAGTGTGTTGTAAATGGTTA+AGG - Chr4:81628817-81628836 None:intergenic 30.0%
TGGATAAGTTGTTGAAAGTT+TGG - Chr4:81630735-81630754 None:intergenic 30.0%
TTACATGCGCTACATTATTA+GGG + Chr4:81629766-81629785 MsG0480023163.01.T01:intron 30.0%
! AACAGTTATGGATTGACATT+AGG + Chr4:81630062-81630081 MsG0480023163.01.T01:intron 30.0%
! AACATCTTCAATGTTTTGCT+TGG - Chr4:81630137-81630156 None:intergenic 30.0%
! ACAGTTATGGATTGACATTA+GGG + Chr4:81630063-81630082 MsG0480023163.01.T01:intron 30.0%
! ACTTTTGTACCTAACCAAAA+TGG - Chr4:81630510-81630529 None:intergenic 30.0%
! ATCATTGGTGATAACAGTTA+TGG + Chr4:81630050-81630069 MsG0480023163.01.T01:intron 30.0%
! CATTATTAGGGATTCTTTTG+TGG + Chr4:81629778-81629797 MsG0480023163.01.T01:intron 30.0%
! CTTTTGTACCTAACCAAAAT+GGG - Chr4:81630509-81630528 None:intergenic 30.0%
! GAAAGTGAAGCATTTACAAT+TGG + Chr4:81630283-81630302 MsG0480023163.01.T01:intron 30.0%
! TGATTACTGATTGACATTGA+AGG + Chr4:81630168-81630187 MsG0480023163.01.T01:intron 30.0%
!! ACCTAGATGGTTTGTTTATT+AGG + Chr4:81630474-81630493 MsG0480023163.01.T01:intron 30.0%
!! CCTAGATGGTTTGTTTATTA+GGG + Chr4:81630475-81630494 MsG0480023163.01.T01:intron 30.0%
!! GATATAGGTGTTTTTGAACA+TGG + Chr4:81630014-81630033 MsG0480023163.01.T01:intron 30.0%
!!! ACTCAAGTTTTAGTTTCGTT+AGG - Chr4:81629285-81629304 None:intergenic 30.0%
!!! AGTTTTGGAAAAGCAAAAGA+AGG + Chr4:81629314-81629333 MsG0480023163.01.T01:intron 30.0%
AATGGTAAAGTTGTCTTTGG+TGG + Chr4:81630256-81630275 MsG0480023163.01.T01:intron 35.0%
ACACTAAAAACCAAGCCAAA+TGG + Chr4:81630238-81630257 MsG0480023163.01.T01:intron 35.0%
ACAGTAGAAGAAAAAGGGTT+AGG + Chr4:81630567-81630586 MsG0480023163.01.T01:intron 35.0%
ATTGAAGATGTTGTCGTCTT+TGG + Chr4:81630145-81630164 MsG0480023163.01.T01:intron 35.0%
CCAAAGACAACTTTACCATT+TGG - Chr4:81630256-81630275 None:intergenic 35.0%
CCAAATGGTAAAGTTGTCTT+TGG + Chr4:81630253-81630272 MsG0480023163.01.T01:intron 35.0%
CCCTAATAAACAAACCATCT+AGG - Chr4:81630478-81630497 None:intergenic 35.0%
CGTAGCTTATAATCTAGCTA+AGG - Chr4:81629248-81629267 None:intergenic 35.0%
GAGTTCCATTACAGTAACAA+TGG + Chr4:81630190-81630209 MsG0480023163.01.T01:intron 35.0%
GGATTTCAGAGCTATTATCA+AGG - Chr4:81629350-81629369 None:intergenic 35.0%
GTTACATGCGCTACATTATT+AGG + Chr4:81629765-81629784 MsG0480023163.01.T01:intron 35.0%
TACTTAGGCATTTCAAGATC+CGG - Chr4:81630803-81630822 None:intergenic 35.0%
TGGATAATAACAAGGCCATT+TGG + Chr4:81630303-81630322 MsG0480023163.01.T01:intron 35.0%
TTTGGAGAAAACGTAGATAG+TGG + Chr4:81629979-81629998 MsG0480023163.01.T01:intron 35.0%
! ATTCTTTTGTGGTTCATGCA+AGG + Chr4:81629789-81629808 MsG0480023163.01.T01:intron 35.0%
! CAGTTATGGATTGACATTAG+GGG + Chr4:81630064-81630083 MsG0480023163.01.T01:intron 35.0%
! CTTTTGCCACAAACTAAAGT+AGG - Chr4:81629687-81629706 None:intergenic 35.0%
! TCATGTTTCTGCAAAAGGAA+AGG - Chr4:81630709-81630728 None:intergenic 35.0%
! TTCTTTTGTGGTTCATGCAA+GGG + Chr4:81629790-81629809 MsG0480023163.01.T01:intron 35.0%
! TTGAGTCATTGAGTGAGTTT+TGG + Chr4:81629299-81629318 MsG0480023163.01.T01:intron 35.0%
!! CTACTTTAGTTTGTGGCAAA+AGG + Chr4:81629685-81629704 MsG0480023163.01.T01:intron 35.0%
AAACATATGCTGATGGAGTG+AGG + Chr4:81630534-81630553 MsG0480023163.01.T01:intron 40.0%
AACATATGCTGATGGAGTGA+GGG + Chr4:81630535-81630554 MsG0480023163.01.T01:intron 40.0%
ACGTTGCTCATAGAATCTTC+AGG - Chr4:81630767-81630786 None:intergenic 40.0%
AGAAGAAAAAGGGTTAGGAG+AGG + Chr4:81630572-81630591 MsG0480023163.01.T01:intron 40.0%
CATGCTTCTCTAACACCAAA+TGG - Chr4:81630321-81630340 None:intergenic 40.0%
CATGTAAACACAGCAGCAAA+AGG + Chr4:81630337-81630356 MsG0480023163.01.T01:intron 40.0%
CCTAACCAAAATGGGTAAGA+TGG - Chr4:81630501-81630520 None:intergenic 40.0%
CTATGAGCAACGTTATTGAC+AGG + Chr4:81630774-81630793 MsG0480023163.01.T01:CDS 40.0%
GAATCTTCAGGAAATGCATC+TGG - Chr4:81630755-81630774 None:intergenic 40.0%
GACAACTTTACCATTTGGCT+TGG - Chr4:81630251-81630270 None:intergenic 40.0%
GACATTAGGGGTGATAACAA+GGG + Chr4:81630076-81630095 MsG0480023163.01.T01:intron 40.0%
GTTAAGGGAACGAAGATCAT+CGG - Chr4:81628801-81628820 None:intergenic 40.0%
TACTCAGATTTCTCCTGACA+TGG + Chr4:81628990-81629009 MsG0480023163.01.T01:CDS 40.0%
TGACATTAGGGGTGATAACA+AGG + Chr4:81630075-81630094 MsG0480023163.01.T01:intron 40.0%
TGGAAGAACACAGAGGATAT+AGG + Chr4:81629999-81630018 MsG0480023163.01.T01:intron 40.0%
! GGTCTTCATGTTTCTGCAAA+AGG - Chr4:81630714-81630733 None:intergenic 40.0%
! TCAGCAACTTTTGCAAGTTG+AGG + Chr4:81629743-81629762 MsG0480023163.01.T01:intron 40.0%
! TCTTTTGTGGTTCATGCAAG+GGG + Chr4:81629791-81629810 MsG0480023163.01.T01:intron 40.0%
!! AAAAGGTGCTACAATTGCTG+AGG + Chr4:81629702-81629721 MsG0480023163.01.T01:intron 40.0%
!! ACTTCGCCTACTTTAGTTTG+TGG + Chr4:81629678-81629697 MsG0480023163.01.T01:intron 40.0%
!! GGTGTTGGTGAAGTTATCAT+TGG + Chr4:81630035-81630054 MsG0480023163.01.T01:intron 40.0%
!! GGTGTTTTTGAACATGGTGT+TGG + Chr4:81630020-81630039 MsG0480023163.01.T01:intron 40.0%
!! TAGATAGTGGAAGAACACAG+AGG + Chr4:81629992-81630011 MsG0480023163.01.T01:intron 40.0%
!!! CCATCTTACCCATTTTGGTT+AGG + Chr4:81630498-81630517 MsG0480023163.01.T01:intron 40.0%
CAACGTTATTGACAGGAGAC+CGG + Chr4:81630781-81630800 MsG0480023163.01.T01:CDS 45.0%
GCTTACATTCAAGAGTTGGG+AGG + Chr4:81629410-81629429 MsG0480023163.01.T01:intron 45.0%
GTGGGAAGTGATGACAAGTA+TGG + Chr4:81630389-81630408 MsG0480023163.01.T01:intron 45.0%
GTTATGACTTCTGCACGTTG+TGG + Chr4:81629222-81629241 MsG0480023163.01.T01:intron 45.0%
TTGTCATCACTTCCCACTCT+TGG - Chr4:81630386-81630405 None:intergenic 45.0%
TTTCAGATTGGGAAGGTTGC+AGG + Chr4:81628918-81628937 MsG0480023163.01.T01:intron 45.0%
! CTTTTGTGGTTCATGCAAGG+GGG + Chr4:81629792-81629811 MsG0480023163.01.T01:intron 45.0%
! TAGGGCCATCTTACCCATTT+TGG + Chr4:81630493-81630512 MsG0480023163.01.T01:intron 45.0%
!!! TCAGGTATTTTTGGCTCGTG+CGG - Chr4:81629456-81629475 None:intergenic 45.0%
!!! TTTCTATATATATATTTTTT+AGG + Chr4:81629147-81629166 MsG0480023163.01.T01:intron 5.0%
ACATTCAAGAGTTGGGAGGC+AGG + Chr4:81629414-81629433 MsG0480023163.01.T01:intron 50.0%
GTGCTACAATTGCTGAGGCA+AGG + Chr4:81629707-81629726 MsG0480023163.01.T01:intron 50.0%
!! CGTGTGTTTCTAGCCATGTC+AGG - Chr4:81629006-81629025 None:intergenic 50.0%
!!! GTATTTTTGGCTCGTGCGGA+GGG - Chr4:81629452-81629471 None:intergenic 50.0%
!!! GGTATTTTTGGCTCGTGCGG+AGG - Chr4:81629453-81629472 None:intergenic 55.0%
GAGTTGGGAGGCAGGCAGTA+TGG + Chr4:81629422-81629441 MsG0480023163.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 81628704 81630823 81628704 ID=MsG0480023163.01;Name=MsG0480023163.01
Chr4 mRNA 81628704 81630823 81628704 ID=MsG0480023163.01.T01;Parent=MsG0480023163.01;Name=MsG0480023163.01.T01;_AED=0.43;_eAED=0.45;_QI=0|0|0|1|1|1|3|0|129
Chr4 exon 81628704 81628837 81628704 ID=MsG0480023163.01.T01:exon:700;Parent=MsG0480023163.01.T01
Chr4 exon 81628924 81629076 81628924 ID=MsG0480023163.01.T01:exon:701;Parent=MsG0480023163.01.T01
Chr4 exon 81630721 81630823 81630721 ID=MsG0480023163.01.T01:exon:702;Parent=MsG0480023163.01.T01
Chr4 CDS 81628704 81628837 81628704 ID=MsG0480023163.01.T01:cds;Parent=MsG0480023163.01.T01
Chr4 CDS 81628924 81629076 81628924 ID=MsG0480023163.01.T01:cds;Parent=MsG0480023163.01.T01
Chr4 CDS 81630721 81630823 81630721 ID=MsG0480023163.01.T01:cds;Parent=MsG0480023163.01.T01
Gene Sequence

>MsG0480023163.01.T01

ATGTCAGAGAAAGAATCAGAATCAGAATCAGAATCAGAATCGATGAAATTAGGTTCAGAAGAGCTTTCGAGTGAGTTTAAAACTCTCGTTAGTTCCGATGATCTTCGTTCCCTTAACCATTTACAACACACTATATTGGGAAGGTTGCAGGACAGTAATGCAGTGTTATCACATTTCAATGATTTCTCTCAACATTGTTTTACTCAGATTTCTCCTGACATGGCTAGAAACACACGTGTCTTGAAGTCTATCAAATCTGATCTTGATTATATTTTTCTTAAACTTAGAAACATGAAGACCAAACTTTCAACAACTTATCCAGATGCATTTCCTGAAGATTCTATGAGCAACGTTATTGACAGGAGACCGGATCTTGAAATGCCTAAGTAA

Protein sequence

>MsG0480023163.01.T01

MSEKESESESESESMKLGSEELSSEFKTLVSSDDLRSLNHLQHTILGRLQDSNAVLSHFNDFSQHCFTQISPDMARNTRVLKSIKSDLDYIFLKLRNMKTKLSTTYPDAFPEDSMSNVIDRRPDLEMPK*