AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040914.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040914.01.T01 MTR_7g100905 100.000 73 0 0 1 73 1 73 9.91e-47 143
MsG0780040914.01.T01 MTR_7g100905 100.000 66 0 0 8 73 3 68 3.43e-41 129
MsG0780040914.01.T01 MTR_7g100880 85.897 78 6 1 1 73 7 84 2.90e-18 71.6
MsG0780040914.01.T01 MTR_7g100890 90.411 73 6 1 1 73 426 497 2.91e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040914.01.T01 AT2G37120 76.190 63 15 0 11 73 14 76 5.53e-15 63.2
MsG0780040914.01.T01 AT3G53370 80.000 60 12 0 14 73 17 76 1.77e-12 57.0

Find 16 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTGTTCTCCTGGTTAT+TGG 0.231388 7:-84208247 MsG0780040914.01.T01:CDS
CCCAGGCTTGATTGTTCTCC+TGG 0.358351 7:-84208254 MsG0780040914.01.T01:CDS
TGGGGAAATGTTTAGAATGC+TGG 0.422488 7:-84208292 MsG0780040914.01.T01:intron
GTTTAGAATGCTGGATCTCA+AGG 0.468079 7:-84208283 MsG0780040914.01.T01:intron
ACCAGGAGAACAATCAAGCC+TGG 0.470447 7:+84208253 None:intergenic
ATTGTTCTCCTGGTTATTGG+TGG 0.489076 7:-84208244 MsG0780040914.01.T01:CDS
TTGCTGTTGACATTTCTCAT+TGG 0.497549 7:-84208220 MsG0780040914.01.T01:CDS
AAGCAAGGAGTCTCTGCACC+TGG 0.518649 7:-84208106 MsG0780040914.01.T01:CDS
GTACAGTGCCTCTACTCTCC+AGG 0.535553 7:+84208088 None:intergenic
TTGTTCTCCTGGTTATTGGT+GGG 0.546594 7:-84208243 MsG0780040914.01.T01:CDS
GCTTTACGTTTATGCACAGA+AGG 0.577488 7:-84208188 MsG0780040914.01.T01:CDS
ACAGCAACCCACCAATAACC+AGG 0.598951 7:+84208236 None:intergenic
CCAGGAGAACAATCAAGCCT+GGG 0.608678 7:+84208254 None:intergenic
GGATCTCAAGGGTTCAACCC+AGG 0.613072 7:-84208271 MsG0780040914.01.T01:CDS
TTTAGAATGCTGGATCTCAA+GGG 0.619147 7:-84208282 MsG0780040914.01.T01:intron
TCTCTGCACCTGGAGAGTAG+AGG 0.653251 7:-84208096 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAATATGAAGAATG+TGG + Chr7:84208170-84208189 None:intergenic 15.0%
!! AAAATAAATATGAAGAATGT+GGG + Chr7:84208169-84208188 None:intergenic 15.0%
!! CAATAAAAAAATATTTCCAA+AGG + Chr7:84209138-84209157 None:intergenic 15.0%
!! GATCTATTCATAATTAAATT+AGG - Chr7:84208340-84208359 MsG0780040914.01.T01:intron 15.0%
!! TTATTTGAGAATAACAATAA+GGG + Chr7:84208729-84208748 None:intergenic 15.0%
!!! GATTTTTTTTTTTCACATTT+AGG - Chr7:84208362-84208381 MsG0780040914.01.T01:intron 15.0%
!! AGATTCTACTTTGAATATAA+TGG - Chr7:84208953-84208972 MsG0780040914.01.T01:intron 20.0%
!! CTTATTTGAGAATAACAATA+AGG + Chr7:84208730-84208749 None:intergenic 20.0%
!!! ACAAAAAAAGCACTTAAATA+AGG + Chr7:84209181-84209200 None:intergenic 20.0%
!!! TTTTCAGATCTTAAAATTCA+TGG - Chr7:84208187-84208206 MsG0780040914.01.T01:CDS 20.0%
!!! TTTTTTTCACATTTAGGTTA+AGG - Chr7:84208368-84208387 MsG0780040914.01.T01:intron 20.0%
! CATATTACTATTTGCATACA+TGG - Chr7:84209070-84209089 MsG0780040914.01.T01:intron 25.0%
! CTCAAAAAAGAAGATGAAAA+AGG - Chr7:84209362-84209381 MsG0780040914.01.T01:intron 25.0%
! GGTTACAAATAACATCAATA+TGG + Chr7:84208708-84208727 None:intergenic 25.0%
!! TTTACATGTCAATTTTCCTT+TGG - Chr7:84209119-84209138 MsG0780040914.01.T01:intron 25.0%
!!! GGAATTGATTTGAAGAAAAT+CGG + Chr7:84208148-84208167 None:intergenic 25.0%
!!! TGCTTTATTAGACACTTTTA+AGG - Chr7:84208441-84208460 MsG0780040914.01.T01:intron 25.0%
!!! TTAATTTGAGAGTGTTTTTG+TGG + Chr7:84208891-84208910 None:intergenic 25.0%
AAAAGAAGATGAAAAAGGAG+AGG - Chr7:84209367-84209386 MsG0780040914.01.T01:intron 30.0%
AACCATTCAACACAAATTGA+TGG + Chr7:84208661-84208680 None:intergenic 30.0%
ATTCTCAAATAAGTCTGTGT+AGG - Chr7:84208737-84208756 MsG0780040914.01.T01:intron 30.0%
ATTGCAGAGCAAATAAAGAA+TGG + Chr7:84209049-84209068 None:intergenic 30.0%
GAAAATGCATATGATAAACC+TGG + Chr7:84208686-84208705 None:intergenic 30.0%
TATAACTGAAAAACCAGTGA+TGG + Chr7:84208305-84208324 None:intergenic 30.0%
TATGAATAGATCCACAAGTA+AGG + Chr7:84208332-84208351 None:intergenic 30.0%
! TTAGATCATGATGATGATGA+TGG - Chr7:84208552-84208571 MsG0780040914.01.T01:intron 30.0%
! TTTTGTGGAGACATACAAAA+GGG + Chr7:84208876-84208895 None:intergenic 30.0%
! TTTTTGTGGAGACATACAAA+AGG + Chr7:84208877-84208896 None:intergenic 30.0%
!!! TAATTTTTTACCTCCATCAC+TGG - Chr7:84208289-84208308 MsG0780040914.01.T01:intron 30.0%
!!! TTCATCTTCTTTTTTGAGAC+TGG + Chr7:84209360-84209379 None:intergenic 30.0%
AGAAAATCGGATCTAAGAAG+AGG + Chr7:84208135-84208154 None:intergenic 35.0%
AGTTATATACGCCTTACTTG+TGG - Chr7:84208318-84208337 MsG0780040914.01.T01:intron 35.0%
ATTGTATTGTACACGCGTTA+AGG - Chr7:84208777-84208796 MsG0780040914.01.T01:intron 35.0%
CGTAGAATATGAGAAAGGTA+AGG + Chr7:84208228-84208247 None:intergenic 35.0%
TGCCATCAATTTGTGTTGAA+TGG - Chr7:84208656-84208675 MsG0780040914.01.T01:intron 35.0%
TTGATTGTTCTCCTGGTTAT+TGG - Chr7:84209252-84209271 MsG0780040914.01.T01:intron 35.0%
! AAGTAGAATCTCTGATTTGC+AGG + Chr7:84208944-84208963 None:intergenic 35.0%
! AGTAGAATCTCTGATTTGCA+GGG + Chr7:84208943-84208962 None:intergenic 35.0%
! CCTTTGTTTCAAAGAATCTC+TGG - Chr7:84208991-84209010 MsG0780040914.01.T01:intron 35.0%
! TCAACACAAATTGATGGCAT+TGG + Chr7:84208655-84208674 None:intergenic 35.0%
! TGAAAGTATGAACGAGCAAA+GGG + Chr7:84208251-84208270 None:intergenic 35.0%
! TTGAAAGTATGAACGAGCAA+AGG + Chr7:84208252-84208271 None:intergenic 35.0%
! TTGCTGTTGACATTTCTCAT+TGG - Chr7:84209279-84209298 MsG0780040914.01.T01:intron 35.0%
! TTTAGAATGCTGGATCTCAA+GGG - Chr7:84209217-84209236 MsG0780040914.01.T01:intron 35.0%
! TTTGAAACAAAGGAGAGAAG+TGG + Chr7:84208984-84209003 None:intergenic 35.0%
! TTTGTGTTGAATGGTTTTCC+AGG - Chr7:84208665-84208684 MsG0780040914.01.T01:intron 35.0%
!! CCAGAGATTCTTTGAAACAA+AGG + Chr7:84208994-84209013 None:intergenic 35.0%
!!! AGTGCTTTTTTTGTTGCTCT+TGG - Chr7:84209187-84209206 MsG0780040914.01.T01:intron 35.0%
!!! GTGCTTTTTTTGTTGCTCTT+GGG - Chr7:84209188-84209207 MsG0780040914.01.T01:intron 35.0%
!!! TGCTTTTTTTGTTGCTCTTG+GGG - Chr7:84209189-84209208 MsG0780040914.01.T01:intron 35.0%
AACTGAAAAACCAGTGATGG+AGG + Chr7:84208302-84208321 None:intergenic 40.0%
AGAAGAGGAGAGAAGAATAC+CGG + Chr7:84208120-84208139 None:intergenic 40.0%
ATTGTTCTCCTGGTTATTGG+TGG - Chr7:84209255-84209274 MsG0780040914.01.T01:intron 40.0%
CATACATGGATGATTGTGAG+TGG - Chr7:84209084-84209103 MsG0780040914.01.T01:intron 40.0%
GCTTTACGTTTATGCACAGA+AGG - Chr7:84209311-84209330 MsG0780040914.01.T01:intron 40.0%
GGGACTGTGAATCATAAATC+AGG + Chr7:84208856-84208875 None:intergenic 40.0%
TTGTTCTCCTGGTTATTGGT+GGG - Chr7:84209256-84209275 MsG0780040914.01.T01:intron 40.0%
! AAGGGCGTAGAATATGAGAA+AGG + Chr7:84208233-84208252 None:intergenic 40.0%
! ATGATGGTGATACTGCAGAA+TGG - Chr7:84208568-84208587 MsG0780040914.01.T01:intron 40.0%
! GTTTAGAATGCTGGATCTCA+AGG - Chr7:84209216-84209235 MsG0780040914.01.T01:intron 40.0%
! TGGGGAAATGTTTAGAATGC+TGG - Chr7:84209207-84209226 MsG0780040914.01.T01:intron 40.0%
!!! ACTGGCTTCTTTTTTCTAGG+AGG + Chr7:84209342-84209361 None:intergenic 40.0%
!!! CTGGCTTCTTTTTTCTAGGA+GGG + Chr7:84209341-84209360 None:intergenic 40.0%
!!! GAGACTGGCTTCTTTTTTCT+AGG + Chr7:84209345-84209364 None:intergenic 40.0%
AAAAAGGAGAGGCTGAAGCA+AGG - Chr7:84209378-84209397 MsG0780040914.01.T01:intron 45.0%
AAGAGGAGAGAAGAATACCG+GGG + Chr7:84208118-84208137 None:intergenic 45.0%
ACATGGATGATTGTGAGTGG+TGG - Chr7:84209087-84209106 MsG0780040914.01.T01:intron 45.0%
GAAGAGGAGAGAAGAATACC+GGG + Chr7:84208119-84208138 None:intergenic 45.0%
GAGAAAGGTAAGGCATGCAA+GGG + Chr7:84208218-84208237 None:intergenic 45.0%
TGAGAAAGGTAAGGCATGCA+AGG + Chr7:84208219-84208238 None:intergenic 45.0%
ACAGCAACCCACCAATAACC+AGG + Chr7:84209266-84209285 None:intergenic 50.0%
ACCAGGAGAACAATCAAGCC+TGG + Chr7:84209249-84209268 None:intergenic 50.0%
AGAGGAGAGAAGAATACCGG+GGG + Chr7:84208117-84208136 None:intergenic 50.0%
CCAGGAGAACAATCAAGCCT+GGG + Chr7:84209248-84209267 None:intergenic 50.0%
GAATACCGGGGGGACTTTAT+CGG + Chr7:84208106-84208125 None:intergenic 50.0%
AAGCAAGGAGTCTCTGCACC+TGG - Chr7:84209393-84209412 MsG0780040914.01.T01:intron 55.0%
CCCAGGCTTGATTGTTCTCC+TGG - Chr7:84209245-84209264 MsG0780040914.01.T01:intron 55.0%
GAGGAGAGAAGAATACCGGG+GGG + Chr7:84208116-84208135 None:intergenic 55.0%
GGATCTCAAGGGTTCAACCC+AGG - Chr7:84209228-84209247 MsG0780040914.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 84208099 84209422 84208099 ID=MsG0780040914.01;Name=MsG0780040914.01
Chr7 mRNA 84208099 84209422 84208099 ID=MsG0780040914.01.T01;Parent=MsG0780040914.01;Name=MsG0780040914.01.T01;_AED=0.43;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|73
Chr7 exon 84208099 84208299 84208099 ID=MsG0780040914.01.T01:exon:40685;Parent=MsG0780040914.01.T01
Chr7 exon 84209402 84209422 84209402 ID=MsG0780040914.01.T01:exon:40684;Parent=MsG0780040914.01.T01
Chr7 CDS 84209402 84209422 84209402 ID=MsG0780040914.01.T01:cds;Parent=MsG0780040914.01.T01
Chr7 CDS 84208099 84208299 84208099 ID=MsG0780040914.01.T01:cds;Parent=MsG0780040914.01.T01
Chr7 mRNA 84208143 84208214 84208143 ID=MsG0780040914.01.T02;Parent=MsG0780040914.01;Name=MsG0780040914.01.T02;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|23
Chr7 exon 84208143 84208214 84208143 ID=MsG0780040914.01.T02:exon:40686;Parent=MsG0780040914.01.T02
Chr7 CDS 84208143 84208214 84208143 ID=MsG0780040914.01.T02:cds;Parent=MsG0780040914.01.T02
Gene Sequence

>MsG0780040914.01.T01

ATGGCCGATAAAGTCCCCCCGAATGCTGGATCTCAAGGGTTCAACCCAGGCTTGATTGTTCTCCTGGTTATTGGTGGGTTGCTGTTGACATTTCTCATTGGAAATTATGCGCTTTACGTTTATGCACAGAAGGCTCTCCCTCCTAGAAAAAAGAAGCCAGTCTCAAAAAAGAAGATGAAAAAGGAGAGGCTGAAGCAAGGAGTCTCTGCACCTGGAGAGTAG

>MsG0780040914.01.T02

ATGCGCTTTACGTTTATGCACAGAAGGCTCTCCCTCCTAGAAAAAAGAAGCCAGTCTCAAAAAAGAAGATGA

Protein sequence

>MsG0780040914.01.T01

MADKVPPNAGSQGFNPGLIVLLVIGGLLLTFLIGNYALYVYAQKALPPRKKKPVSKKKMKKERLKQGVSAPGE*

>MsG0780040914.01.T02

MRFTFMHRRLSLLEKRSQSQKRR*