Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008288.01.T02 | XP_003595113.1 | 98.649 | 74 | 1 | 0 | 1 | 74 | 102 | 175 | 2.15E-42 | 145 |
| MsG0280008288.01.T01 | XP_003595113.1 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 6.93E-119 | 343 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008288.01.T01 | Q7ZWJ3 | 44.366 | 142 | 77 | 2 | 34 | 174 | 7 | 147 | 3.89E-37 | 128 |
| MsG0280008288.01.T02 | Q7ZWJ3 | 42.857 | 70 | 40 | 0 | 4 | 73 | 78 | 147 | 1.41E-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008288.01.T01 | G7IIC1 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 3.31e-119 | 343 |
| MsG0280008288.01.T02 | G7IIC1 | 98.649 | 74 | 1 | 0 | 1 | 74 | 102 | 175 | 1.03e-42 | 145 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048507.01 | MsG0280008288.01 | 0.816318 | 5.881834e-52 | 4.154372e-49 |
| MsG0080048748.01 | MsG0280008288.01 | -0.820059 | 8.378076e-53 | 6.560346e-50 |
| MsG0080048845.01 | MsG0280008288.01 | -0.805238 | 1.470403e-49 | 7.739235e-47 |
| MsG0080048938.01 | MsG0280008288.01 | -0.826402 | 2.766256e-54 | 2.591668e-51 |
| MsG0080049002.01 | MsG0280008288.01 | -0.804157 | 2.471826e-49 | 1.265318e-46 |
| MsG0080049130.01 | MsG0280008288.01 | -0.811539 | 6.656329e-51 | 4.131366e-48 |
| MsG0180000017.01 | MsG0280008288.01 | -0.818516 | 1.881914e-52 | 1.411743e-49 |
| MsG0180000489.01 | MsG0280008288.01 | -0.856656 | 2.775816e-62 | 6.672758e-59 |
| MsG0180000490.01 | MsG0280008288.01 | -0.800162 | 1.640262e-48 | 7.583794e-46 |
| MsG0180000491.01 | MsG0280008288.01 | -0.835309 | 1.809202e-56 | 2.204093e-53 |
| MsG0180000549.01 | MsG0280008288.01 | -0.801704 | 7.943074e-49 | 3.818716e-46 |
| MsG0180000599.01 | MsG0280008288.01 | -0.852239 | 5.257978e-61 | 1.091709e-57 |
| MsG0180000843.01 | MsG0280008288.01 | -0.832234 | 1.061860e-55 | 1.179636e-52 |
| MsG0180000942.01 | MsG0280008288.01 | -0.855975 | 4.398473e-62 | 1.033717e-58 |
| MsG0180000977.01 | MsG0280008288.01 | -0.817369 | 3.417147e-52 | 2.483890e-49 |
| MsG0180001371.01 | MsG0280008288.01 | -0.804756 | 1.854294e-49 | 9.639981e-47 |
| MsG0180001654.01 | MsG0280008288.01 | -0.801515 | 8.682657e-49 | 4.154620e-46 |
| MsG0180002700.01 | MsG0280008288.01 | -0.844106 | 9.281971e-59 | 1.483081e-55 |
| MsG0180004103.01 | MsG0280008288.01 | -0.819965 | 8.803699e-53 | 6.875331e-50 |
| MsG0180004106.01 | MsG0280008288.01 | -0.821234 | 4.499843e-53 | 3.640177e-50 |
| MsG0180004518.01 | MsG0280008288.01 | -0.807382 | 5.193518e-50 | 2.888582e-47 |
| MsG0180004934.01 | MsG0280008288.01 | -0.819564 | 1.087083e-52 | 8.394911e-50 |
| MsG0180005130.01 | MsG0280008288.01 | 0.803565 | 3.280892e-49 | 1.654216e-46 |
| MsG0180005339.01 | MsG0280008288.01 | -0.860297 | 2.278589e-63 | 6.198048e-60 |
| MsG0180005803.01 | MsG0280008288.01 | -0.822566 | 2.211616e-53 | 1.858050e-50 |
| MsG0180006136.01 | MsG0280008288.01 | -0.804107 | 2.532212e-49 | 1.294552e-46 |
| MsG0280006553.01 | MsG0280008288.01 | -0.807106 | 5.943307e-50 | 3.281717e-47 |
| MsG0280006706.01 | MsG0280008288.01 | -0.802418 | 5.664312e-49 | 2.773374e-46 |
| MsG0280007874.01 | MsG0280008288.01 | 0.805133 | 1.546394e-49 | 8.117431e-47 |
| MsG0280008288.01 | MsG0280008419.01 | -0.805683 | 1.185777e-49 | 6.313305e-47 |
| MsG0280008288.01 | MsG0280008709.01 | -0.814483 | 1.505487e-51 | 1.011442e-48 |
| MsG0280008288.01 | MsG0280009435.01 | -0.834120 | 3.602178e-56 | 4.234093e-53 |
| MsG0280008288.01 | MsG0280009436.01 | -0.823825 | 1.124392e-53 | 9.788412e-51 |
| MsG0280008288.01 | MsG0280009756.01 | -0.815462 | 9.133314e-52 | 6.301342e-49 |
| MsG0280008288.01 | MsG0280009757.01 | -0.802209 | 6.253896e-49 | 3.045548e-46 |
| MsG0280008288.01 | MsG0280009953.01 | -0.801087 | 1.062154e-48 | 5.028104e-46 |
| MsG0280008288.01 | MsG0280011073.01 | -0.837301 | 5.635740e-57 | 7.289345e-54 |
| MsG0280008288.01 | MsG0280011110.01 | 0.836449 | 9.295118e-57 | 1.171820e-53 |
| MsG0280008288.01 | MsG0380011662.01 | -0.841732 | 3.976109e-58 | 5.895474e-55 |
| MsG0280008288.01 | MsG0380012567.01 | -0.817953 | 2.523560e-52 | 1.863862e-49 |
| MsG0280008288.01 | MsG0380012868.01 | -0.835051 | 2.101875e-56 | 2.540479e-53 |
| MsG0280008288.01 | MsG0380014386.01 | -0.819741 | 9.902385e-53 | 7.685122e-50 |
| MsG0280008288.01 | MsG0380014517.01 | -0.808724 | 2.691177e-50 | 1.550177e-47 |
| MsG0280008288.01 | MsG0380014909.01 | -0.828208 | 1.021598e-54 | 1.008689e-51 |
| MsG0280008288.01 | MsG0380015847.01 | 0.832544 | 8.898682e-56 | 9.977716e-53 |
| MsG0280008288.01 | MsG0380016057.01 | -0.820163 | 7.929892e-53 | 6.227240e-50 |
| MsG0280008288.01 | MsG0380016188.01 | 0.803852 | 2.860151e-49 | 1.452708e-46 |
| MsG0280008288.01 | MsG0380016527.01 | -0.828691 | 7.809078e-55 | 7.819125e-52 |
| MsG0280008288.01 | MsG0380017338.01 | -0.814772 | 1.299418e-51 | 8.797531e-49 |
| MsG0280008288.01 | MsG0380017437.01 | 0.820492 | 6.665670e-53 | 5.282103e-50 |
| MsG0280008288.01 | MsG0480018226.01 | -0.806760 | 7.033295e-50 | 3.849006e-47 |
| MsG0280008288.01 | MsG0480018525.01 | 0.819689 | 1.018123e-52 | 7.889584e-50 |
| MsG0280008288.01 | MsG0480018951.01 | -0.808400 | 3.155229e-50 | 1.802365e-47 |
| MsG0280008288.01 | MsG0480019065.01 | -0.807405 | 5.137310e-50 | 2.858995e-47 |
| MsG0280008288.01 | MsG0480020229.01 | -0.812264 | 4.626358e-51 | 2.927893e-48 |
| MsG0280008288.01 | MsG0480020473.01 | -0.814224 | 1.718034e-51 | 1.146079e-48 |
| MsG0280008288.01 | MsG0480021207.01 | -0.822652 | 2.112316e-53 | 1.778890e-50 |
| MsG0280008288.01 | MsG0480021406.01 | -0.813211 | 2.871283e-51 | 1.863402e-48 |
| MsG0280008288.01 | MsG0480022065.01 | -0.811739 | 6.022851e-51 | 3.757904e-48 |
| MsG0280008288.01 | MsG0480022162.01 | -0.815581 | 8.591624e-52 | 5.946715e-49 |
| MsG0280008288.01 | MsG0480022617.01 | -0.826822 | 2.196658e-54 | 2.082817e-51 |
| MsG0280008288.01 | MsG0480022664.01 | -0.815965 | 7.054029e-52 | 4.934153e-49 |
| MsG0280008288.01 | MsG0480022918.01 | -0.826956 | 2.040262e-54 | 1.942189e-51 |
| MsG0280008288.01 | MsG0480022954.01 | 0.803727 | 3.035857e-49 | 1.536973e-46 |
| MsG0280008288.01 | MsG0480023190.01 | -0.829626 | 4.634182e-55 | 4.768971e-52 |
| MsG0280008288.01 | MsG0580024201.01 | -0.808865 | 2.510561e-50 | 1.451599e-47 |
| MsG0280008288.01 | MsG0580024285.01 | -0.820885 | 5.414380e-53 | 4.337810e-50 |
| MsG0280008288.01 | MsG0580024340.01 | -0.816147 | 6.423959e-52 | 4.515911e-49 |
| MsG0280008288.01 | MsG0580024608.01 | -0.807973 | 3.890483e-50 | 2.197390e-47 |
| MsG0280008288.01 | MsG0580024717.01 | -0.821026 | 5.025146e-53 | 4.042080e-50 |
| MsG0280008288.01 | MsG0580024930.01 | 0.808345 | 3.242360e-50 | 1.849429e-47 |
| MsG0280008288.01 | MsG0580025301.01 | -0.800025 | 1.748529e-48 | 8.056731e-46 |
| MsG0280008288.01 | MsG0580025644.01 | -0.814219 | 1.722441e-51 | 1.148848e-48 |
| MsG0280008288.01 | MsG0580026498.01 | -0.820733 | 5.869394e-53 | 4.682063e-50 |
| MsG0280008288.01 | MsG0580027394.01 | -0.802903 | 4.497172e-49 | 2.229257e-46 |
| MsG0280008288.01 | MsG0580028093.01 | -0.809198 | 2.129913e-50 | 1.242611e-47 |
| MsG0280008288.01 | MsG0580028118.01 | 0.856063 | 4.143971e-62 | 9.768158e-59 |
| MsG0280008288.01 | MsG0580028419.01 | -0.828515 | 8.613781e-55 | 8.581095e-52 |
| MsG0280008288.01 | MsG0580028422.01 | -0.815458 | 9.150991e-52 | 6.312863e-49 |
| MsG0280008288.01 | MsG0580028495.01 | -0.823594 | 1.273623e-53 | 1.101480e-50 |
| MsG0280008288.01 | MsG0580028925.01 | -0.819850 | 9.353347e-53 | 7.280856e-50 |
| MsG0280008288.01 | MsG0580029262.01 | 0.803037 | 4.219577e-49 | 2.098689e-46 |
| MsG0280008288.01 | MsG0580029734.01 | -0.803733 | 3.028293e-49 | 1.533382e-46 |
| MsG0280008288.01 | MsG0580029912.01 | -0.821380 | 4.163279e-53 | 3.381511e-50 |
| MsG0280008288.01 | MsG0680030464.01 | -0.800696 | 1.276835e-48 | 5.984157e-46 |
| MsG0280008288.01 | MsG0680030514.01 | -0.815100 | 1.098756e-51 | 7.505212e-49 |
| MsG0280008288.01 | MsG0680030680.01 | -0.828825 | 7.247927e-55 | 7.285376e-52 |
| MsG0280008288.01 | MsG0680031996.01 | -0.832440 | 9.439817e-56 | 1.055192e-52 |
| MsG0280008288.01 | MsG0680031997.01 | -0.807936 | 3.961994e-50 | 2.235534e-47 |
| MsG0280008288.01 | MsG0680032000.01 | -0.806463 | 8.127720e-50 | 4.414397e-47 |
| MsG0280008288.01 | MsG0680032002.01 | -0.819058 | 1.417756e-52 | 1.079580e-49 |
| MsG0280008288.01 | MsG0680032006.01 | -0.804100 | 2.539862e-49 | 1.298257e-46 |
| MsG0280008288.01 | MsG0680032254.01 | -0.830646 | 2.612645e-55 | 2.769428e-52 |
| MsG0280008288.01 | MsG0680032413.01 | -0.831684 | 1.451519e-55 | 1.586802e-52 |
| MsG0280008288.01 | MsG0680034759.01 | 0.804688 | 1.915331e-49 | 9.939714e-47 |
| MsG0280008288.01 | MsG0680035664.01 | -0.850184 | 2.000999e-60 | 3.887102e-57 |
| MsG0280008288.01 | MsG0780035965.01 | -0.806003 | 1.015657e-49 | 5.452182e-47 |
| MsG0280008288.01 | MsG0780036509.01 | -0.815853 | 7.472277e-52 | 5.210396e-49 |
| MsG0280008288.01 | MsG0780036620.01 | -0.804417 | 2.182522e-49 | 1.124667e-46 |
| MsG0280008288.01 | MsG0780036792.01 | -0.822826 | 1.924056e-53 | 1.628416e-50 |
| MsG0280008288.01 | MsG0780037052.01 | 0.809677 | 1.681096e-50 | 9.933374e-48 |
| MsG0280008288.01 | MsG0780037351.01 | -0.815672 | 8.201359e-52 | 5.690921e-49 |
| MsG0280008288.01 | MsG0780037363.01 | -0.820969 | 5.177875e-53 | 4.158304e-50 |
| MsG0280008288.01 | MsG0780039161.01 | -0.810244 | 1.269364e-50 | 7.612544e-48 |
| MsG0280008288.01 | MsG0780039795.01 | -0.837278 | 5.711542e-57 | 7.382122e-54 |
| MsG0280008288.01 | MsG0780039806.01 | -0.827838 | 1.254352e-54 | 1.225062e-51 |
| MsG0280008288.01 | MsG0780039964.01 | -0.819686 | 1.019748e-52 | 7.901487e-50 |
| MsG0280008288.01 | MsG0780040278.01 | -0.820521 | 6.564238e-53 | 5.205676e-50 |
| MsG0280008288.01 | MsG0780040881.01 | 0.811919 | 5.502216e-51 | 3.449417e-48 |
| MsG0280008288.01 | MsG0780040914.01 | 0.840480 | 8.479538e-58 | 1.209356e-54 |
| MsG0280008288.01 | MsG0780041159.01 | -0.843744 | 1.160484e-58 | 1.833230e-55 |
| MsG0280008288.01 | MsG0880042000.01 | -0.826119 | 3.231565e-54 | 3.003155e-51 |
| MsG0280008288.01 | MsG0880042086.01 | -0.851385 | 9.185137e-61 | 1.856039e-57 |
| MsG0280008288.01 | MsG0880042256.01 | -0.820518 | 6.574590e-53 | 5.213447e-50 |
| MsG0280008288.01 | MsG0880042415.01 | -0.826728 | 2.313062e-54 | 2.187209e-51 |
| MsG0280008288.01 | MsG0880042416.01 | -0.835190 | 1.938121e-56 | 2.352537e-53 |
| MsG0280008288.01 | MsG0880042417.01 | -0.803963 | 2.713001e-49 | 1.381891e-46 |
| MsG0280008288.01 | MsG0880043107.01 | -0.851962 | 6.304600e-61 | 1.297306e-57 |
| MsG0280008288.01 | MsG0880043235.01 | -0.835598 | 1.528997e-56 | 1.878628e-53 |
| MsG0280008288.01 | MsG0880043250.01 | -0.821069 | 4.911558e-53 | 3.955412e-50 |
| MsG0280008288.01 | MsG0880043278.01 | -0.827150 | 1.834250e-54 | 1.755905e-51 |
| MsG0280008288.01 | MsG0880044105.01 | -0.817920 | 2.566913e-52 | 1.894282e-49 |
| MsG0280008288.01 | MsG0880044304.01 | -0.813574 | 2.389625e-51 | 1.566289e-48 |
| MsG0280008288.01 | MsG0880044619.01 | -0.810760 | 9.819276e-51 | 5.970271e-48 |
| MsG0280008288.01 | MsG0880044625.01 | -0.809935 | 1.479782e-50 | 8.803279e-48 |
| MsG0280008288.01 | MsG0880045031.01 | -0.807444 | 5.039409e-50 | 2.807305e-47 |
| MsG0280008288.01 | MsG0880045927.01 | -0.804344 | 2.260157e-49 | 1.162532e-46 |
| MsG0280008288.01 | MsG0880046048.01 | -0.800475 | 1.416253e-48 | 6.600524e-46 |
| MsG0280008288.01 | MsG0880046324.01 | -0.811883 | 5.601437e-51 | 3.508287e-48 |
| MsG0280008288.01 | MsG0880046334.01 | 0.822485 | 2.310497e-53 | 1.936484e-50 |
| MsG0280008288.01 | MsG0880046434.01 | 0.826994 | 1.998042e-54 | 1.904065e-51 |
| MsG0280008288.01 | MsG0880046533.01 | -0.819883 | 9.192919e-53 | 7.162643e-50 |
| MsG0280008288.01 | MsG0880047091.01 | 0.802491 | 5.471531e-49 | 2.683756e-46 |
| MsG0280008288.01 | MsG0880047111.01 | -0.811910 | 5.526598e-51 | 3.463878e-48 |
| MsG0280008288.01 | MsG0880047394.01 | -0.804109 | 2.529458e-49 | 1.293213e-46 |
| MsG0280008288.01 | MsG0880047481.01 | 0.881287 | 2.720051e-70 | 1.584139e-66 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008288.01.T02 | MTR_2g038390 | 98.649 | 74 | 1 | 0 | 1 | 74 | 102 | 175 | 2.60e-46 | 145 |
| MsG0280008288.01.T01 | MTR_2g038390 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 8.39e-123 | 343 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008288.01.T02 | AT4G29870 | 87.671 | 73 | 9 | 0 | 1 | 73 | 99 | 171 | 3.97e-41 | 132 |
| MsG0280008288.01.T02 | AT2G19340 | 83.562 | 73 | 12 | 0 | 1 | 73 | 100 | 172 | 1.58e-39 | 128 |
| MsG0280008288.01.T02 | AT2G19340 | 83.607 | 61 | 10 | 0 | 1 | 61 | 100 | 160 | 3.44e-31 | 108 |
| MsG0280008288.01.T01 | AT4G29870 | 78.788 | 165 | 35 | 0 | 10 | 174 | 7 | 171 | 2.85e-92 | 266 |
| MsG0280008288.01.T01 | AT2G19340 | 74.138 | 174 | 43 | 1 | 1 | 174 | 1 | 172 | 7.49e-92 | 265 |
| MsG0280008288.01.T01 | AT2G19340 | 76.471 | 153 | 36 | 0 | 10 | 162 | 8 | 160 | 1.32e-83 | 245 |
Find 80 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTTCGTGCTTGGTGGAAT+TGG | 0.312895 | 2:-28711268 | MsG0280008288.01.T01:CDS |
| ATCTTGCTCTTGATCGGAAC+CGG | 0.323803 | 2:-28711231 | MsG0280008288.01.T01:CDS |
| CGTCCTCCTCACCTACTTCA+TGG | 0.335652 | 2:-28711440 | MsG0280008288.01.T01:CDS |
| ACGGTGGTGGTTTGTTGAAC+CGG | 0.340478 | 2:+28711604 | None:intergenic |
| CGAAGACGAGGTTGGAGGAG+GGG | 0.345915 | 2:+28711467 | None:intergenic |
| GGAAGGAGAAGGGGAGGATC+CGG | 0.355642 | 2:+28711533 | None:intergenic |
| TGGCTTAACGGCGCCGGTGT+AGG | 0.406356 | 2:+28711354 | None:intergenic |
| AGGGGAGGATCCGGAGGAGG+TGG | 0.408799 | 2:+28711542 | None:intergenic |
| GAGGAGGGGAGGGAGAAGGA+TGG | 0.411678 | 2:+28711481 | None:intergenic |
| CATGAAGACGACTGGCTTAA+CGG | 0.420596 | 2:+28711342 | None:intergenic |
| AGGAGAAGGGGAGGATCCGG+AGG | 0.425705 | 2:+28711536 | None:intergenic |
| TGTTCGTGCTTGGTGGAATT+GGG | 0.427782 | 2:-28711267 | MsG0280008288.01.T01:CDS |
| GATCCGGAGGAGGTGGAAGA+TGG | 0.429609 | 2:+28711549 | None:intergenic |
| TCTTGCATTGAGCCGATGCC+GGG | 0.432581 | 2:+28711379 | None:intergenic |
| ATCTTGCATTGAGCCGATGC+CGG | 0.434052 | 2:+28711378 | None:intergenic |
| GACGGAGGCGTGGTGGGCGG+AGG | 0.434820 | 2:+28711512 | None:intergenic |
| AGGAGGGGAGGGAGAAGGAT+GGG | 0.438169 | 2:+28711482 | None:intergenic |
| TTCATGTCCGGACGTGTTAA+CGG | 0.438277 | 2:-28711325 | MsG0280008288.01.T01:CDS |
| GGCTTAACGGCGCCGGTGTA+GGG | 0.445049 | 2:+28711355 | None:intergenic |
| ATCATTGAAGGTCTCTCGTC+AGG | 0.449642 | 2:-28711295 | MsG0280008288.01.T01:CDS |
| GTTGGAGGAGGGGAGGGAGA+AGG | 0.450711 | 2:+28711477 | None:intergenic |
| ATCCCATCTTCCACCTCCTC+CGG | 0.450876 | 2:-28711552 | MsG0280008288.01.T01:CDS |
| CGGTGGTGGTTTGTTGAACC+GGG | 0.452712 | 2:+28711605 | None:intergenic |
| TCAGGATTCATGTTCGTGCT+TGG | 0.455117 | 2:-28711277 | MsG0280008288.01.T01:CDS |
| TGGAATTGGGATTATTATGC+TGG | 0.456292 | 2:-28711254 | MsG0280008288.01.T01:CDS |
| GTGGGCGGAGGAAGGAGAAG+GGG | 0.459226 | 2:+28711524 | None:intergenic |
| GGCGAAGACGAGGTTGGAGG+AGG | 0.461934 | 2:+28711465 | None:intergenic |
| GACGAGGTTGGAGGAGGGGA+GGG | 0.466931 | 2:+28711471 | None:intergenic |
| GGCGGAGGAAGGAGAAGGGG+AGG | 0.466959 | 2:+28711527 | None:intergenic |
| GGATTCATGTTCGTGCTTGG+TGG | 0.470744 | 2:-28711274 | MsG0280008288.01.T01:CDS |
| GTTTGAGACGGAGGCGTGGT+GGG | 0.471062 | 2:+28711506 | None:intergenic |
| AGAAGGGGAGGATCCGGAGG+AGG | 0.471530 | 2:+28711539 | None:intergenic |
| GCGAAGACGAGGTTGGAGGA+GGG | 0.471961 | 2:+28711466 | None:intergenic |
| AAGCCAGTCGTCTTCATGTC+CGG | 0.477097 | 2:-28711337 | MsG0280008288.01.T01:CDS |
| TGCTGGATCTTGCTCTTGAT+CGG | 0.479498 | 2:-28711237 | MsG0280008288.01.T01:CDS |
| AGACGAGGTTGGAGGAGGGG+AGG | 0.481721 | 2:+28711470 | None:intergenic |
| GGATGGATCGGTGACGGTGG+TGG | 0.485863 | 2:+28711591 | None:intergenic |
| AAGGTTTCTTATGCAACTGC+TGG | 0.491757 | 2:-28711196 | MsG0280008288.01.T01:CDS |
| CAAATTCAACTGAGATAAGC+CGG | 0.494114 | 2:+28711115 | None:intergenic |
| TGGTGGGCGGAGGAAGGAGA+AGG | 0.498639 | 2:+28711522 | None:intergenic |
| TCGATTGATGCGGCGGAGGA+TGG | 0.505246 | 2:+28711574 | None:intergenic |
| GAGGCGTGGTGGGCGGAGGA+AGG | 0.507826 | 2:+28711516 | None:intergenic |
| AGAAGGATGGGAGTTTGAGA+CGG | 0.508005 | 2:+28711494 | None:intergenic |
| AAACCTTGACAGAGCGATCC+CGG | 0.511299 | 2:+28711212 | None:intergenic |
| ATCCGGAGGAGGTGGAAGAT+GGG | 0.511311 | 2:+28711550 | None:intergenic |
| GACGACTGGCTTAACGGCGC+CGG | 0.515557 | 2:+28711348 | None:intergenic |
| ATCGTCGAACCACCCGGCAT+CGG | 0.518980 | 2:-28711391 | MsG0280008288.01.T01:CDS |
| ACCTACTTCATGGTCGTCTC+CGG | 0.520194 | 2:-28711430 | MsG0280008288.01.T01:CDS |
| TGAAGTAGGTGAGGAGGACG+AGG | 0.520328 | 2:+28711443 | None:intergenic |
| CTTCGTTCGTATCAAGATCC+CGG | 0.526209 | 2:-28711134 | MsG0280008288.01.T01:CDS |
| GACGAGGGCGAAGACGAGGT+TGG | 0.528267 | 2:+28711459 | None:intergenic |
| TCTTGCTCTTGATCGGAACC+GGG | 0.540417 | 2:-28711230 | MsG0280008288.01.T01:CDS |
| AACGGTCAGTACATCATTGA+AGG | 0.541670 | 2:-28711307 | MsG0280008288.01.T01:CDS |
| GGTGGGCGGAGGAAGGAGAA+GGG | 0.547643 | 2:+28711523 | None:intergenic |
| GTACTGACCGTTAACACGTC+CGG | 0.550984 | 2:+28711318 | None:intergenic |
| GGGATCGATTGATGCGGCGG+AGG | 0.552918 | 2:+28711570 | None:intergenic |
| TGATGCGGCGGAGGATGGAT+CGG | 0.563992 | 2:+28711579 | None:intergenic |
| TGAGACGGAGGCGTGGTGGG+CGG | 0.564410 | 2:+28711509 | None:intergenic |
| AGTTTGAGACGGAGGCGTGG+TGG | 0.568521 | 2:+28711505 | None:intergenic |
| GGCGGAGGATGGATCGGTGA+CGG | 0.572830 | 2:+28711585 | None:intergenic |
| GGGAGTTTGAGACGGAGGCG+TGG | 0.580940 | 2:+28711502 | None:intergenic |
| GACATCATCGTCGAACCACC+CGG | 0.581154 | 2:-28711397 | MsG0280008288.01.T01:CDS |
| GAGGGCGAAGACGAGGTTGG+AGG | 0.581282 | 2:+28711462 | None:intergenic |
| GATGATGTCGTAGACGATGC+CGG | 0.583805 | 2:+28711411 | None:intergenic |
| GAACCGGGATCGCTCTGTCA+AGG | 0.595385 | 2:-28711215 | MsG0280008288.01.T01:CDS |
| CGTCCGGACATGAAGACGAC+TGG | 0.599107 | 2:+28711334 | None:intergenic |
| GGAGGACGAGGGCGAAGACG+AGG | 0.607340 | 2:+28711455 | None:intergenic |
| AAATTCAACTGAGATAAGCC+GGG | 0.608585 | 2:+28711116 | None:intergenic |
| GAAATCCAAATCAGAAAACC+CGG | 0.611081 | 2:-28711623 | MsG0280008288.01.T01:CDS |
| GATGGGATCGATTGATGCGG+CGG | 0.623752 | 2:+28711567 | None:intergenic |
| CGACCATGAAGTAGGTGAGG+AGG | 0.625138 | 2:+28711437 | None:intergenic |
| GCCGGAGACGACCATGAAGT+AGG | 0.628375 | 2:+28711429 | None:intergenic |
| TGCATTGAGCCGATGCCGGG+TGG | 0.630436 | 2:+28711382 | None:intergenic |
| GGAGGATGGATCGGTGACGG+TGG | 0.635929 | 2:+28711588 | None:intergenic |
| AACATAAGCAATGACAACAC+TGG | 0.647800 | 2:+28711168 | None:intergenic |
| AGACGACCATGAAGTAGGTG+AGG | 0.650471 | 2:+28711434 | None:intergenic |
| AGGATGGGAGTTTGAGACGG+AGG | 0.662863 | 2:+28711497 | None:intergenic |
| TCAATGCAAGATCCCTACAC+CGG | 0.666564 | 2:-28711367 | MsG0280008288.01.T01:CDS |
| GAAGATGGGATCGATTGATG+CGG | 0.694236 | 2:+28711564 | None:intergenic |
| GAAGTAGGTGAGGAGGACGA+GGG | 0.704348 | 2:+28711444 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATAAGCAATGACAACAC+TGG | + | Chr2:28711580-28711599 | None:intergenic | 35.0% | |
| GAAATCCAAATCAGAAAACC+CGG | - | Chr2:28711122-28711141 | MsG0280008288.01.T01:CDS | 35.0% | |
| TGGAATTGGGATTATTATGC+TGG | - | Chr2:28711491-28711510 | MsG0280008288.01.T01:CDS | 35.0% | |
| AACGGTCAGTACATCATTGA+AGG | - | Chr2:28711438-28711457 | MsG0280008288.01.T01:CDS | 40.0% | |
| AAGGTTTCTTATGCAACTGC+TGG | - | Chr2:28711549-28711568 | MsG0280008288.01.T01:CDS | 40.0% | |
| ! | TTGAACCGGGTTTTCTGATT+TGG | + | Chr2:28711130-28711149 | None:intergenic | 40.0% |
| AGAAGGATGGGAGTTTGAGA+CGG | + | Chr2:28711254-28711273 | None:intergenic | 45.0% | |
| ATCATTGAAGGTCTCTCGTC+AGG | - | Chr2:28711450-28711469 | MsG0280008288.01.T01:CDS | 45.0% | |
| ATCTTGCTCTTGATCGGAAC+CGG | - | Chr2:28711514-28711533 | MsG0280008288.01.T01:CDS | 45.0% | |
| CTTCGTTCGTATCAAGATCC+CGG | - | Chr2:28711611-28711630 | MsG0280008288.01.T01:CDS | 45.0% | |
| GAAGATGGGATCGATTGATG+CGG | + | Chr2:28711184-28711203 | None:intergenic | 45.0% | |
| TCAATGCAAGATCCCTACAC+CGG | - | Chr2:28711378-28711397 | MsG0280008288.01.T01:CDS | 45.0% | |
| TCAGGATTCATGTTCGTGCT+TGG | - | Chr2:28711468-28711487 | MsG0280008288.01.T01:CDS | 45.0% | |
| TGCTGGATCTTGCTCTTGAT+CGG | - | Chr2:28711508-28711527 | MsG0280008288.01.T01:CDS | 45.0% | |
| TTCATGTCCGGACGTGTTAA+CGG | - | Chr2:28711420-28711439 | MsG0280008288.01.T01:CDS | 45.0% | |
| ! | ATGTTCGTGCTTGGTGGAAT+TGG | - | Chr2:28711477-28711496 | MsG0280008288.01.T01:CDS | 45.0% |
| ! | TGTTCGTGCTTGGTGGAATT+GGG | - | Chr2:28711478-28711497 | MsG0280008288.01.T01:CDS | 45.0% |
| !! | CATGAAGACGACTGGCTTAA+CGG | + | Chr2:28711406-28711425 | None:intergenic | 45.0% |
| AAACCTTGACAGAGCGATCC+CGG | + | Chr2:28711536-28711555 | None:intergenic | 50.0% | |
| AAGCCAGTCGTCTTCATGTC+CGG | - | Chr2:28711408-28711427 | MsG0280008288.01.T01:CDS | 50.0% | |
| ACCTACTTCATGGTCGTCTC+CGG | - | Chr2:28711315-28711334 | MsG0280008288.01.T01:CDS | 50.0% | |
| AGACGACCATGAAGTAGGTG+AGG | + | Chr2:28711314-28711333 | None:intergenic | 50.0% | |
| ATCTTGCATTGAGCCGATGC+CGG | + | Chr2:28711370-28711389 | None:intergenic | 50.0% | |
| GATGATGTCGTAGACGATGC+CGG | + | Chr2:28711337-28711356 | None:intergenic | 50.0% | |
| GGATTCATGTTCGTGCTTGG+TGG | - | Chr2:28711471-28711490 | MsG0280008288.01.T01:CDS | 50.0% | |
| TCTTGCTCTTGATCGGAACC+GGG | - | Chr2:28711515-28711534 | MsG0280008288.01.T01:CDS | 50.0% | |
| ! | GTACTGACCGTTAACACGTC+CGG | + | Chr2:28711430-28711449 | None:intergenic | 50.0% |
| !! | ACGGTGGTGGTTTGTTGAAC+CGG | + | Chr2:28711144-28711163 | None:intergenic | 50.0% |
| ATCCCATCTTCCACCTCCTC+CGG | - | Chr2:28711193-28711212 | MsG0280008288.01.T01:CDS | 55.0% | |
| ATCCGGAGGAGGTGGAAGAT+GGG | + | Chr2:28711198-28711217 | None:intergenic | 55.0% | |
| CGACCATGAAGTAGGTGAGG+AGG | + | Chr2:28711311-28711330 | None:intergenic | 55.0% | |
| CGTCCTCCTCACCTACTTCA+TGG | - | Chr2:28711305-28711324 | MsG0280008288.01.T01:CDS | 55.0% | |
| GAAGTAGGTGAGGAGGACGA+GGG | + | Chr2:28711304-28711323 | None:intergenic | 55.0% | |
| GACATCATCGTCGAACCACC+CGG | - | Chr2:28711348-28711367 | MsG0280008288.01.T01:CDS | 55.0% | |
| GATGGGATCGATTGATGCGG+CGG | + | Chr2:28711181-28711200 | None:intergenic | 55.0% | |
| TCTTGCATTGAGCCGATGCC+GGG | + | Chr2:28711369-28711388 | None:intergenic | 55.0% | |
| TGAAGTAGGTGAGGAGGACG+AGG | + | Chr2:28711305-28711324 | None:intergenic | 55.0% | |
| ! | AGGATGGGAGTTTGAGACGG+AGG | + | Chr2:28711251-28711270 | None:intergenic | 55.0% |
| !! | CGGTGGTGGTTTGTTGAACC+GGG | + | Chr2:28711143-28711162 | None:intergenic | 55.0% |
| AGGAGGGGAGGGAGAAGGAT+GGG | + | Chr2:28711266-28711285 | None:intergenic | 60.0% | |
| ATCGTCGAACCACCCGGCAT+CGG | - | Chr2:28711354-28711373 | MsG0280008288.01.T01:CDS | 60.0% | |
| CGAAGACGAGGTTGGAGGAG+GGG | + | Chr2:28711281-28711300 | None:intergenic | 60.0% | |
| CGTCCGGACATGAAGACGAC+TGG | + | Chr2:28711414-28711433 | None:intergenic | 60.0% | |
| GAACCGGGATCGCTCTGTCA+AGG | - | Chr2:28711530-28711549 | MsG0280008288.01.T01:CDS | 60.0% | |
| GATCCGGAGGAGGTGGAAGA+TGG | + | Chr2:28711199-28711218 | None:intergenic | 60.0% | |
| GCCGGAGACGACCATGAAGT+AGG | + | Chr2:28711319-28711338 | None:intergenic | 60.0% | |
| GCGAAGACGAGGTTGGAGGA+GGG | + | Chr2:28711282-28711301 | None:intergenic | 60.0% | |
| GGAAGGAGAAGGGGAGGATC+CGG | + | Chr2:28711215-28711234 | None:intergenic | 60.0% | |
| TCGATTGATGCGGCGGAGGA+TGG | + | Chr2:28711174-28711193 | None:intergenic | 60.0% | |
| TGATGCGGCGGAGGATGGAT+CGG | + | Chr2:28711169-28711188 | None:intergenic | 60.0% | |
| ! | AGTTTGAGACGGAGGCGTGG+TGG | + | Chr2:28711243-28711262 | None:intergenic | 60.0% |
| ! | GTTTGAGACGGAGGCGTGGT+GGG | + | Chr2:28711242-28711261 | None:intergenic | 60.0% |
| AGAAGGGGAGGATCCGGAGG+AGG | + | Chr2:28711209-28711228 | None:intergenic | 65.0% | |
| AGACGAGGTTGGAGGAGGGG+AGG | + | Chr2:28711278-28711297 | None:intergenic | 65.0% | |
| AGGAGAAGGGGAGGATCCGG+AGG | + | Chr2:28711212-28711231 | None:intergenic | 65.0% | |
| GACGAGGGCGAAGACGAGGT+TGG | + | Chr2:28711289-28711308 | None:intergenic | 65.0% | |
| GACGAGGTTGGAGGAGGGGA+GGG | + | Chr2:28711277-28711296 | None:intergenic | 65.0% | |
| GAGGAGGGGAGGGAGAAGGA+TGG | + | Chr2:28711267-28711286 | None:intergenic | 65.0% | |
| GAGGGCGAAGACGAGGTTGG+AGG | + | Chr2:28711286-28711305 | None:intergenic | 65.0% | |
| GGCGAAGACGAGGTTGGAGG+AGG | + | Chr2:28711283-28711302 | None:intergenic | 65.0% | |
| GGCGGAGGATGGATCGGTGA+CGG | + | Chr2:28711163-28711182 | None:intergenic | 65.0% | |
| GGGATCGATTGATGCGGCGG+AGG | + | Chr2:28711178-28711197 | None:intergenic | 65.0% | |
| GGTGGGCGGAGGAAGGAGAA+GGG | + | Chr2:28711225-28711244 | None:intergenic | 65.0% | |
| GTGGGCGGAGGAAGGAGAAG+GGG | + | Chr2:28711224-28711243 | None:intergenic | 65.0% | |
| GTTGGAGGAGGGGAGGGAGA+AGG | + | Chr2:28711271-28711290 | None:intergenic | 65.0% | |
| TGCATTGAGCCGATGCCGGG+TGG | + | Chr2:28711366-28711385 | None:intergenic | 65.0% | |
| ! | GGAGGATGGATCGGTGACGG+TGG | + | Chr2:28711160-28711179 | None:intergenic | 65.0% |
| ! | GGATGGATCGGTGACGGTGG+TGG | + | Chr2:28711157-28711176 | None:intergenic | 65.0% |
| ! | GGGAGTTTGAGACGGAGGCG+TGG | + | Chr2:28711246-28711265 | None:intergenic | 65.0% |
| ! | TGGTGGGCGGAGGAAGGAGA+AGG | + | Chr2:28711226-28711245 | None:intergenic | 65.0% |
| !! | GACGACTGGCTTAACGGCGC+CGG | + | Chr2:28711400-28711419 | None:intergenic | 65.0% |
| !! | GGCTTAACGGCGCCGGTGTA+GGG | + | Chr2:28711393-28711412 | None:intergenic | 65.0% |
| !! | TGGCTTAACGGCGCCGGTGT+AGG | + | Chr2:28711394-28711413 | None:intergenic | 65.0% |
| AGGGGAGGATCCGGAGGAGG+TGG | + | Chr2:28711206-28711225 | None:intergenic | 70.0% | |
| GGAGGACGAGGGCGAAGACG+AGG | + | Chr2:28711293-28711312 | None:intergenic | 70.0% | |
| GGCGGAGGAAGGAGAAGGGG+AGG | + | Chr2:28711221-28711240 | None:intergenic | 70.0% | |
| ! | TGAGACGGAGGCGTGGTGGG+CGG | + | Chr2:28711239-28711258 | None:intergenic | 70.0% |
| !! | GAGGCGTGGTGGGCGGAGGA+AGG | + | Chr2:28711232-28711251 | None:intergenic | 75.0% |
| !!! | GACGGAGGCGTGGTGGGCGG+AGG | + | Chr2:28711236-28711255 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 28711120 | 28711647 | 28711120 | ID=MsG0280008288.01;Name=MsG0280008288.01 |
| Chr2 | mRNA | 28711120 | 28711647 | 28711120 | ID=MsG0280008288.01.T01;Parent=MsG0280008288.01;Name=MsG0280008288.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|175 |
| Chr2 | exon | 28711120 | 28711647 | 28711120 | ID=MsG0280008288.01.T01:exon:35209;Parent=MsG0280008288.01.T01 |
| Chr2 | CDS | 28711120 | 28711647 | 28711120 | ID=MsG0280008288.01.T01:cds;Parent=MsG0280008288.01.T01 |
| Chr2 | mRNA | 28711120 | 28711344 | 28711120 | ID=MsG0280008288.01.T02;Parent=MsG0280008288.01;Name=MsG0280008288.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|74 |
| Chr2 | exon | 28711120 | 28711344 | 28711120 | ID=MsG0280008288.01.T02:exon:35210;Parent=MsG0280008288.01.T02 |
| Chr2 | CDS | 28711120 | 28711344 | 28711120 | ID=MsG0280008288.01.T02:cds;Parent=MsG0280008288.01.T02 |
| Gene Sequence |
| Protein sequence |
