Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041175.01.T01 | KEH37700.1 | 91.837 | 49 | 4 | 0 | 1 | 49 | 26 | 74 | 1.66E-23 | 93.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041175.01.T01 | A0A072VHS5 | 91.837 | 49 | 4 | 0 | 1 | 49 | 26 | 74 | 7.95e-24 | 93.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048727.01 | MsG0780041175.01 | 0.808579 | 2.889439e-50 | 1.658178e-47 |
| MsG0080048876.01 | MsG0780041175.01 | 0.821815 | 3.304216e-53 | 2.717044e-50 |
| MsG0080049039.01 | MsG0780041175.01 | 0.835202 | 1.925384e-56 | 2.337912e-53 |
| MsG0080049040.01 | MsG0780041175.01 | 0.809159 | 2.171612e-50 | 1.265592e-47 |
| MsG0080049054.01 | MsG0780041175.01 | 0.806614 | 7.551910e-50 | 4.117319e-47 |
| MsG0080049068.01 | MsG0780041175.01 | 0.821431 | 4.053445e-53 | 3.297123e-50 |
| MsG0180000878.01 | MsG0780041175.01 | 0.838066 | 3.585163e-57 | 4.747745e-54 |
| MsG0180001085.01 | MsG0780041175.01 | 0.825640 | 4.197995e-54 | 3.848474e-51 |
| MsG0180001175.01 | MsG0780041175.01 | 0.811436 | 7.009102e-51 | 4.338552e-48 |
| MsG0180001270.01 | MsG0780041175.01 | 0.825953 | 3.537939e-54 | 3.272641e-51 |
| MsG0180003526.01 | MsG0780041175.01 | 0.816469 | 5.441448e-52 | 3.858864e-49 |
| MsG0180004163.01 | MsG0780041175.01 | 0.805675 | 1.190612e-49 | 6.337495e-47 |
| MsG0180004276.01 | MsG0780041175.01 | 0.803643 | 3.160974e-49 | 1.596835e-46 |
| MsG0180004448.01 | MsG0780041175.01 | 0.806152 | 9.451467e-50 | 5.092744e-47 |
| MsG0180004733.01 | MsG0780041175.01 | 0.829558 | 4.812990e-55 | 4.943013e-52 |
| MsG0180004739.01 | MsG0780041175.01 | 0.813499 | 2.481795e-51 | 1.623470e-48 |
| MsG0180005349.01 | MsG0780041175.01 | 0.811932 | 5.466150e-51 | 3.427995e-48 |
| MsG0180005492.01 | MsG0780041175.01 | 0.818149 | 2.279209e-52 | 1.692499e-49 |
| MsG0180005961.01 | MsG0780041175.01 | 0.866069 | 3.733224e-65 | 1.241846e-61 |
| MsG0780040284.01 | MsG0780041175.01 | 0.839028 | 2.023726e-57 | 2.759760e-54 |
| MsG0780040331.01 | MsG0780041175.01 | 0.843200 | 1.621734e-58 | 2.518134e-55 |
| MsG0780041174.01 | MsG0780041175.01 | 0.838582 | 2.639270e-57 | 3.550802e-54 |
| MsG0780041175.01 | MsG0780041480.01 | 0.827931 | 1.191275e-54 | 1.166682e-51 |
| MsG0780041175.01 | MsG0880042890.01 | 0.813277 | 2.777234e-51 | 1.805673e-48 |
| MsG0780041175.01 | MsG0880044392.01 | 0.824651 | 7.190610e-54 | 6.409159e-51 |
| MsG0780041175.01 | MsG0880044414.01 | 0.841037 | 6.057134e-58 | 8.789304e-55 |
| MsG0780041175.01 | MsG0880045101.01 | 0.826115 | 3.237552e-54 | 3.008431e-51 |
| MsG0780041175.01 | MsG0880046267.01 | 0.851379 | 9.220748e-61 | 1.862952e-57 |
| MsG0780041175.01 | MsG0880046765.01 | 0.821298 | 4.349616e-53 | 3.524811e-50 |
| MsG0780041175.01 | MsG0880047168.01 | 0.800822 | 1.203496e-48 | 5.658522e-46 |
| MsG0780041175.01 | MsG0880047587.01 | 0.859384 | 4.293032e-63 | 1.131806e-59 |
| MsG0780041175.01 | MsG0880047656.01 | 0.806094 | 9.720391e-50 | 5.229905e-47 |
| MsG0280010661.01 | MsG0780041175.01 | 0.811482 | 6.848299e-51 | 4.244199e-48 |
| MsG0380012801.01 | MsG0780041175.01 | 0.809264 | 2.061976e-50 | 1.205053e-47 |
| MsG0380014588.01 | MsG0780041175.01 | 0.814381 | 1.585821e-51 | 1.062481e-48 |
| MsG0680032057.01 | MsG0780041175.01 | 0.822449 | 2.355450e-53 | 1.972197e-50 |
| MsG0680033377.01 | MsG0780041175.01 | 0.835658 | 1.475795e-56 | 1.816596e-53 |
| MsG0680034064.01 | MsG0780041175.01 | 0.800799 | 1.216425e-48 | 5.715998e-46 |
| MsG0480018644.01 | MsG0780041175.01 | 0.810399 | 1.175164e-50 | 7.076159e-48 |
| MsG0480019221.01 | MsG0780041175.01 | 0.800947 | 1.134456e-48 | 5.351279e-46 |
| MsG0480019751.01 | MsG0780041175.01 | 0.836704 | 8.007225e-57 | 1.017154e-53 |
| MsG0480019956.01 | MsG0780041175.01 | 0.818103 | 2.334397e-52 | 1.731232e-49 |
| MsG0480020408.01 | MsG0780041175.01 | 0.805305 | 1.423543e-49 | 7.505467e-47 |
| MsG0480020690.01 | MsG0780041175.01 | 0.823771 | 1.157686e-53 | 1.006360e-50 |
| MsG0480020753.01 | MsG0780041175.01 | 0.829838 | 4.115253e-55 | 4.262045e-52 |
| MsG0480021682.01 | MsG0780041175.01 | 0.843849 | 1.087590e-58 | 1.723911e-55 |
| MsG0480021754.01 | MsG0780041175.01 | 0.800917 | 1.150939e-48 | 5.424809e-46 |
| MsG0480022647.01 | MsG0780041175.01 | 0.805515 | 1.285911e-49 | 6.816436e-47 |
| MsG0480023314.01 | MsG0780041175.01 | 0.846372 | 2.263225e-59 | 3.887097e-56 |
| MsG0480023444.01 | MsG0780041175.01 | 0.803625 | 3.188953e-49 | 1.610223e-46 |
| MsG0480023661.01 | MsG0780041175.01 | 0.804386 | 2.215096e-49 | 1.140564e-46 |
| MsG0580024873.01 | MsG0780041175.01 | 0.821321 | 4.297257e-53 | 3.484611e-50 |
| MsG0380015603.01 | MsG0780041175.01 | 0.825668 | 4.135615e-54 | 3.794362e-51 |
| MsG0380016192.01 | MsG0780041175.01 | 0.812155 | 4.887758e-51 | 3.084155e-48 |
| MsG0380016836.01 | MsG0780041175.01 | 0.800310 | 1.530071e-48 | 7.101757e-46 |
| MsG0380017028.01 | MsG0780041175.01 | 0.805666 | 1.195713e-49 | 6.363206e-47 |
| MsG0380017703.01 | MsG0780041175.01 | 0.858737 | 6.706739e-63 | 1.729092e-59 |
| MsG0580027887.01 | MsG0780041175.01 | 0.832816 | 7.615043e-56 | 8.607821e-53 |
| MsG0580028335.01 | MsG0780041175.01 | 0.811193 | 7.911456e-51 | 4.865711e-48 |
| MsG0680030627.01 | MsG0780041175.01 | 0.834091 | 3.662216e-56 | 4.300945e-53 |
| MsG0280006805.01 | MsG0780041175.01 | 0.819437 | 1.162064e-52 | 8.942087e-50 |
| MsG0280007831.01 | MsG0780041175.01 | 0.824731 | 6.887649e-54 | 6.153014e-51 |
| MsG0280008451.01 | MsG0780041175.01 | 0.824051 | 9.952586e-54 | 8.718451e-51 |
| MsG0280008591.01 | MsG0780041175.01 | 0.844735 | 6.288434e-59 | 1.024818e-55 |
| MsG0280010376.01 | MsG0780041175.01 | 0.817077 | 3.975544e-52 | 2.866687e-49 |
| MsG0680035731.01 | MsG0780041175.01 | 0.804703 | 1.901792e-49 | 9.873020e-47 |
| MsG0680035791.01 | MsG0780041175.01 | 0.810301 | 1.233667e-50 | 7.409219e-48 |
| MsG0780037184.01 | MsG0780041175.01 | 0.822473 | 2.325058e-53 | 1.948060e-50 |
| MsG0780038784.01 | MsG0780041175.01 | 0.804014 | 2.646439e-49 | 1.349771e-46 |
| MsG0780038807.01 | MsG0780041175.01 | 0.825086 | 5.677875e-54 | 5.123999e-51 |
| MsG0780039675.01 | MsG0780041175.01 | 0.823313 | 1.481472e-53 | 1.271073e-50 |
| MsG0780039746.01 | MsG0780041175.01 | 0.817252 | 3.630364e-52 | 2.630331e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041175.01.T01 | MTR_2g045305 | 91.837 | 49 | 4 | 0 | 1 | 49 | 26 | 74 | 2.02e-27 | 93.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCGGATGACCCGTAATT+TGG | 0.230833 | 7:-87586100 | None:intergenic |
| GCTTGTTTAAATTATGCTTT+TGG | 0.265699 | 7:+87586179 | MsG0780041175.01.T01:CDS |
| TGGGATAAACTCCATGTATT+GGG | 0.296146 | 7:-87586131 | None:intergenic |
| TTAAACAAGCATTAATGCTT+TGG | 0.325309 | 7:-87586166 | None:intergenic |
| GATGACCCGTAATTTGGATT+TGG | 0.339971 | 7:-87586094 | None:intergenic |
| CCACAAAATCCAACTGCTCC+TGG | 0.380071 | 7:+87586058 | MsG0780041175.01.T01:CDS |
| AAAGTTCCAAATCCAAATTA+CGG | 0.387715 | 7:+87586088 | MsG0780041175.01.T01:CDS |
| CATTAATGCTTTGGAAATCC+TGG | 0.397775 | 7:-87586157 | None:intergenic |
| AAGTTCCAAATCCAAATTAC+GGG | 0.421179 | 7:+87586089 | MsG0780041175.01.T01:CDS |
| TGCTTTGGAAATCCTGGAGT+TGG | 0.430753 | 7:-87586151 | None:intergenic |
| TTGGGATAAACTCCATGTAT+TGG | 0.431348 | 7:-87586132 | None:intergenic |
| TTTGGAACTTTAATAACTCC+AGG | 0.479422 | 7:-87586076 | None:intergenic |
| TTAATAACTCCAGGAGCAGT+TGG | 0.480346 | 7:-87586067 | None:intergenic |
| GCTTTGGAAATCCTGGAGTT+GGG | 0.481865 | 7:-87586150 | None:intergenic |
| ATGGAGTTTATCCCAACTCC+AGG | 0.524184 | 7:+87586139 | MsG0780041175.01.T01:CDS |
| TTCTACTCAACCCCAATACA+TGG | 0.535611 | 7:+87586120 | MsG0780041175.01.T01:CDS |
| GGGATAAACTCCATGTATTG+GGG | 0.600281 | 7:-87586130 | None:intergenic |
| GTATTGGGGTTGAGTAGAAG+CGG | 0.629007 | 7:-87586116 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGTTCCAAATCCAAATTA+CGG | + | Chr7:87586088-87586107 | MsG0780041175.01.T01:CDS | 25.0% |
| ! | TTAAACAAGCATTAATGCTT+TGG | - | Chr7:87586169-87586188 | None:intergenic | 25.0% |
| !! | GCTTGTTTAAATTATGCTTT+TGG | + | Chr7:87586179-87586198 | MsG0780041175.01.T01:CDS | 25.0% |
| AAGTTCCAAATCCAAATTAC+GGG | + | Chr7:87586089-87586108 | MsG0780041175.01.T01:CDS | 30.0% | |
| ! | TTTGGAACTTTAATAACTCC+AGG | - | Chr7:87586079-87586098 | None:intergenic | 30.0% |
| TGGGATAAACTCCATGTATT+GGG | - | Chr7:87586134-87586153 | None:intergenic | 35.0% | |
| TTGGGATAAACTCCATGTAT+TGG | - | Chr7:87586135-87586154 | None:intergenic | 35.0% | |
| !! | CATTAATGCTTTGGAAATCC+TGG | - | Chr7:87586160-87586179 | None:intergenic | 35.0% |
| GATGACCCGTAATTTGGATT+TGG | - | Chr7:87586097-87586116 | None:intergenic | 40.0% | |
| GGGATAAACTCCATGTATTG+GGG | - | Chr7:87586133-87586152 | None:intergenic | 40.0% | |
| TTAATAACTCCAGGAGCAGT+TGG | - | Chr7:87586070-87586089 | None:intergenic | 40.0% | |
| TTCTACTCAACCCCAATACA+TGG | + | Chr7:87586120-87586139 | MsG0780041175.01.T01:CDS | 40.0% | |
| ATGGAGTTTATCCCAACTCC+AGG | + | Chr7:87586139-87586158 | MsG0780041175.01.T01:CDS | 45.0% | |
| GTATTGGGGTTGAGTAGAAG+CGG | - | Chr7:87586119-87586138 | None:intergenic | 45.0% | |
| !! | GCTTTGGAAATCCTGGAGTT+GGG | - | Chr7:87586153-87586172 | None:intergenic | 45.0% |
| !! | TGCTTTGGAAATCCTGGAGT+TGG | - | Chr7:87586154-87586173 | None:intergenic | 45.0% |
| CCACAAAATCCAACTGCTCC+TGG | + | Chr7:87586058-87586077 | MsG0780041175.01.T01:CDS | 50.0% | |
| GAAGCGGATGACCCGTAATT+TGG | - | Chr7:87586103-87586122 | None:intergenic | 50.0% | |
| ! | CCAGGAGCAGTTGGATTTTG+TGG | - | Chr7:87586061-87586080 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 87586053 | 87586211 | 87586053 | ID=MsG0780041175.01;Name=MsG0780041175.01 |
| Chr7 | mRNA | 87586053 | 87586211 | 87586053 | ID=MsG0780041175.01.T01;Parent=MsG0780041175.01;Name=MsG0780041175.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|52 |
| Chr7 | exon | 87586053 | 87586211 | 87586053 | ID=MsG0780041175.01.T01:exon:52254;Parent=MsG0780041175.01.T01 |
| Chr7 | CDS | 87586053 | 87586211 | 87586053 | ID=MsG0780041175.01.T01:cds;Parent=MsG0780041175.01.T01 |
| Gene Sequence |
| Protein sequence |
