AlfalfaGEDB | MsG0580025231.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025231.01

KEGG: K15102
GO: -
PFAM: PF00153

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025231.01.T01	XP_024639264.1	99.656	291	1	0	6	296	1	291	0	599

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025231.01.T01	Q7DNC3	65.753	292	99	1	6	296	1	292	6.38E-150	425

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025231.01.T01	A0A396HM95	99.656	291	1	0	6	296	1	291	0.0	599

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048773.01	MsG0580025231.01	0.830092	3.567423e-55	3.720909e-52
MsG0080048845.01	MsG0580025231.01	0.813880	2.045809e-51	1.352134e-48
MsG0080048849.01	MsG0580025231.01	0.802362	5.816322e-49	2.843769e-46
MsG0080048938.01	MsG0580025231.01	0.821918	3.126446e-53	2.578331e-50
MsG0080049002.01	MsG0580025231.01	0.816473	5.431160e-52	3.851978e-49
MsG0080049068.01	MsG0580025231.01	0.803617	3.200739e-49	1.615855e-46
MsG0180000114.01	MsG0580025231.01	0.800665	1.295292e-48	6.066196e-46
MsG0180000599.01	MsG0580025231.01	0.807327	5.335819e-50	2.963495e-47
MsG0180000784.01	MsG0580025231.01	0.822863	1.886633e-53	1.598350e-50
MsG0180000785.01	MsG0580025231.01	0.812413	4.293484e-51	2.728188e-48

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PPI

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Gene1	Gene2	Type
MsG0780040054.01	MsG0580025231.01	PPI
MsG0780040054.01	MsG0580025231.01	PPI
MsG0780040638.01	MsG0580025231.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025231.01.T01	MTR_5g024770	92.096	291	5	1	6	296	1	273	0.0	540
MsG0580025231.01.T01	MTR_4g094578	62.411	282	100	2	21	296	24	305	1.11e-129	371
MsG0580025231.01.T01	MTR_8g099910	49.296	284	140	1	17	296	64	347	1.65e-99	296
MsG0580025231.01.T01	MTR_7g083790	49.470	283	139	1	18	296	74	356	1.14e-98	295
MsG0580025231.01.T01	MTR_6g033280	47.857	280	142	1	21	296	58	337	4.28e-95	285
MsG0580025231.01.T01	MTR_5g073040	27.143	280	181	5	27	291	40	311	9.34e-20	88.2
MsG0580025231.01.T01	MTR_4g057850	25.163	306	193	11	8	290	7	299	8.05e-16	76.6
MsG0580025231.01.T01	MTR_7g064980	26.437	174	120	2	27	192	135	308	2.79e-13	69.3
MsG0580025231.01.T01	MTR_5g009500	26.129	310	178	11	27	294	39	339	4.11e-13	69.3
MsG0580025231.01.T01	MTR_8g027975	24.927	341	202	10	1	296	24	355	6.25e-13	68.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025231.01.T01	AT2G17270	65.753	292	99	1	6	296	1	292	6.51e-151	425
MsG0580025231.01.T01	AT3G48850	48.410	283	142	1	18	296	63	345	9.81e-102	302
MsG0580025231.01.T01	AT5G14040	49.158	297	146	2	5	296	60	356	3.62e-101	301
MsG0580025231.01.T01	AT1G07030	26.786	280	183	6	27	291	41	313	6.07e-19	85.9
MsG0580025231.01.T01	AT5G01340	26.936	297	189	9	14	290	7	295	1.71e-18	84.3
MsG0580025231.01.T01	AT2G30160	26.246	301	186	8	14	291	31	318	1.88e-17	81.6
MsG0580025231.01.T01	AT3G53940	28.161	174	117	2	27	192	181	354	1.27e-15	76.6
MsG0580025231.01.T01	AT2G37890	27.586	174	118	2	27	192	153	326	1.60e-14	73.2
MsG0580025231.01.T01	AT3G20240	24.742	291	186	9	27	291	53	336	1.73e-12	67.4
MsG0580025231.01.T01	AT4G01100	26.728	217	125	6	11	198	83	294	2.67e-12	66.6

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Find 73 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCCGAGAGTCTTGTTC+TGG	0.432484	5:+15594978	MsG0580025231.01.T01:CDS
AAAGCTACCAACGGATCCAG+CGG	0.665155	5:-15594911	None:intergenic
AAATACCAGAACAAGACTCT+CGG	0.618357	5:-15594983	None:intergenic
AAATACTGTAATACTTAATA+GGG	0.401083	5:-15593523	None:intergenic
AAATGGACACAGAGCTAAAT+TGG	0.274987	5:-15593750	None:intergenic
AACAAGGGCCTTCTGTCCTG+TGG	0.579264	5:+15593573	MsG0580025231.01.T01:CDS
AACATGCCCTTAGCATAACT+AGG	0.649459	5:-15593803	None:intergenic
AACCATTGCAAGGTTATAGA+AGG	0.364536	5:-15595153	None:intergenic
AAGACTCCTGGTAAATAAAT+TGG	0.271424	5:-15595106	None:intergenic
AATATACAAGACTTCTGTGC+TGG	0.548042	5:-15593697	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTTCCGGACATTAAGT+AGG	-	Chr5:15594288-15594307	None:intergenic	30.0%
AAAGATGTTTAGTGGGAAAT+TGG	+	Chr5:15594167-15594186	MsG0580025231.01.T01:intron	30.0%
AAATCATTCTCATCCATGAA+AGG	-	Chr5:15592899-15592918	None:intergenic	30.0%
AACATAACTCTCAAGCATAA+AGG	-	Chr5:15592985-15593004	None:intergenic	30.0%
AACTGTCCAACAAAAAAAAC+AGG	-	Chr5:15594767-15594786	None:intergenic	30.0%
AAGACTCCTGGTAAATAAAT+TGG	-	Chr5:15595109-15595128	None:intergenic	30.0%
ACATAACTCTCAAGCATAAA+GGG	-	Chr5:15592984-15593003	None:intergenic	30.0%
ACTTGATTGATCAGGTTATA+GGG	+	Chr5:15594366-15594385	MsG0580025231.01.T01:intron	30.0%
AGAGTTATGTTTCACACTAA+TGG	+	Chr5:15592994-15593013	MsG0580025231.01.T01:intron	30.0%
AGCTATAAGTCTATAACTCA+TGG	+	Chr5:15594710-15594729	MsG0580025231.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	15592418	15595212	15592418	ID=MsG0580025231.01;Name=MsG0580025231.01
Chr5	mRNA	15592418	15595212	15592418	ID=MsG0580025231.01.T01;Parent=MsG0580025231.01;Name=MsG0580025231.01.T01;_AED=0.49;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|5\|0\|296
Chr5	exon	15592418	15592570	15592418	ID=MsG0580025231.01.T01:exon:6257;Parent=MsG0580025231.01.T01
Chr5	exon	15593518	15593867	15593518	ID=MsG0580025231.01.T01:exon:6258;Parent=MsG0580025231.01.T01
Chr5	exon	15593968	15594011	15593968	ID=MsG0580025231.01.T01:exon:6259;Parent=MsG0580025231.01.T01
Chr5	exon	15594785	15594999	15594785	ID=MsG0580025231.01.T01:exon:6260;Parent=MsG0580025231.01.T01
Chr5	exon	15595084	15595212	15595084	ID=MsG0580025231.01.T01:exon:6261;Parent=MsG0580025231.01.T01
Chr5	CDS	15592418	15592570	15592418	ID=MsG0580025231.01.T01:cds;Parent=MsG0580025231.01.T01
Chr5	CDS	15593518	15593867	15593518	ID=MsG0580025231.01.T01:cds;Parent=MsG0580025231.01.T01
Chr5	CDS	15593968	15594011	15593968	ID=MsG0580025231.01.T01:cds;Parent=MsG0580025231.01.T01
Chr5	CDS	15594785	15594999	15594785	ID=MsG0580025231.01.T01:cds;Parent=MsG0580025231.01.T01
Chr5	CDS	15595084	15595212	15595084	ID=MsG0580025231.01.T01:cds;Parent=MsG0580025231.01.T01

Gene Sequence

>MsG0580025231.01.T01

CTGAAGAAAGAGATCATGGCGGCCATGGAAGGAGGAATCTCACAGGAATTAACGCCAAGGTATTATGCCCTTTGCACCATTGGAGGAATGCTCAGTGCTGGTACCACTCATCTTGCTACAACTCCTCTTGATGTTTTGAAAGTTAACATGCAGGTGCACCCTATTAAGTATTACAGTATTTCGTCATGCTTTACTACGTTATTGAGGGAACAAGGGCCTTCTGTCCTGTGGAAAGGATGGACAGGCAAGTTCTTTGGCTATGGTGCTCAAGGAGGATGTAGATTTGGTCTGTATGAATATTTTAAGGGAGTTTACTCCAATGTATTGGTAGACCAGCACAGAAGTCTTGTATATTTTCTTAGTAGTGCATCTGCTGAAGTATTTGCCAATTTAGCTCTGTGTCCATTTGAAGCTGTTAAAGTCAGGGTTCAAGCACAACCTAGTTATGCTAAGGGCATGTTTGACGGCTTTCCAAAGGTATATGCAGCAGAAGGCGCACGAGGGTTCTACCGTGGACTTGTTCCACTTTTGGGTCGAAACATTCCATTTTCCATGGTTATGTTCTCAACATTCGAGCATTCTGTTGATTTTTTGTATCGCAATGTTGTCAAAAGAAAAAAGGAGGAATGTTCAAAAGCTCAACAGCTAGGTGTGACATGTTTAGCTGGTTATACCGCTGGATCCGTTGGTAGCTTTGTTTCTAATCCTGCTGACAATATAGTAGCTTCTCTTTATAACAGAAAAGCCGAGAGTCTTGTTCTGGCTATCAGAAAAATTGGGCTAGCCAATTTATTTACCAGGAGTCTTCCTATTAGAATGTTGCTGGTTGGCCCTTCTATAACCTTGCAATGGTTTTTCTACGACACTATCAAAGTTTTAGGTGGACTGTAA

Protein sequence

>MsG0580025231.01.T01

LKKEIMAAMEGGISQELTPRYYALCTIGGMLSAGTTHLATTPLDVLKVNMQVHPIKYYSISSCFTTLLREQGPSVLWKGWTGKFFGYGAQGGCRFGLYEYFKGVYSNVLVDQHRSLVYFLSSASAEVFANLALCPFEAVKVRVQAQPSYAKGMFDGFPKVYAAEGARGFYRGLVPLLGRNIPFSMVMFSTFEHSVDFLYRNVVKRKKEECSKAQQLGVTCLAGYTAGSVGSFVSNPADNIVASLYNRKAESLVLAIRKIGLANLFTRSLPIRMLLVGPSITLQWFFYDTIKVLGGL*