AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1466590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466590 MtrunA17_Chr5g0394301 100.000 84 0 0 1 84 1 84 1.79e-55 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466590 AT5G26240.2 61.176 85 28 3 1 84 1 81 1.66e-26 101
Msa1466590 AT5G26240.1 61.176 85 28 3 1 84 1 81 1.78e-26 101

Find 26 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCGATGATGACGGCGTT+GGG 0.366873 tig0047679:+718 Msa1466590:CDS
TTTCACCACCCGCACATGTT+TGG 0.374368 tig0047679:+411 Msa1466590:five_prime_UTR
GCTGTATGTGAGTTACTTGC+TGG 0.459222 tig0047679:+954 Msa1466590:CDS
CGTTTGGATTAAGTTGTGTT+TGG 0.471687 tig0047679:-560 Msa1466590:intergenic
CATAAGATTAGAAACTCTTA+AGG 0.471949 tig0047679:+363 Msa1466590:five_prime_UTR
GTCCTCGATGATGACGGCGT+TGG 0.474922 tig0047679:+717 Msa1466590:CDS
GTTACTTGCTGGTGGTGAAA+TGG 0.478899 tig0047679:+965 Msa1466590:CDS
AAAGGTGATTCATCGGAGTT+CGG 0.503410 tig0047679:-693 Msa1466590:intergenic
CAGCATCGTCGTTGGTCGAT+CGG 0.515921 tig0047679:-616 Msa1466590:intergenic
CGAACGAACGACACTGTTAG+TGG 0.517956 tig0047679:+495 Msa1466590:five_prime_UTR
CTGTCGAATCATATTCAAAA+CGG 0.522595 tig0047679:+636 Msa1466590:CDS
TCGAATCTGCGAGACTCGCT+TGG 0.526282 tig0047679:+661 Msa1466590:CDS
TTGAGGTTCCAAACATGTGC+GGG 0.543848 tig0047679:-419 Msa1466590:intergenic
ACACTGTTAGTGGTGGATAG+AGG 0.548071 tig0047679:+505 Msa1466590:five_prime_UTR
TGATTCGACAGCATCGTCGT+TGG 0.579753 tig0047679:-624 Msa1466590:intergenic
CCGTCATCATCGAGGACGAA+AGG 0.595112 tig0047679:-711 Msa1466590:intergenic
TTCATAGTCAAGACTCTCAA+CGG 0.598773 tig0047679:-766 Msa1466590:intergenic
CAGAATGAAAGAAAGAATTG+AGG 0.606881 tig0047679:-436 Msa1466590:intergenic
CCTTTCGTCCTCGATGATGA+CGG 0.613284 tig0047679:+711 Msa1466590:CDS
GAGGACGAAAGGTGATTCAT+CGG 0.613288 tig0047679:-700 Msa1466590:intergenic
GTATGTGAGTTACTTGCTGG+TGG 0.618042 tig0047679:+957 Msa1466590:CDS
GGGATTCTTAAGAAGAACGA+TGG 0.624518 tig0047679:+738 Msa1466590:CDS
ATTGAGGTTCCAAACATGTG+CGG 0.657226 tig0047679:-420 Msa1466590:intergenic
ACGAACGACACTGTTAGTGG+TGG 0.665127 tig0047679:+498 Msa1466590:five_prime_UTR
TCCCAACGCCGTCATCATCG+AGG 0.707360 tig0047679:-719 Msa1466580:intergenic
AGGTTCCAAACATGTGCGGG+TGG 0.707945 tig0047679:-416 Msa1466580:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTGTTCAATGATAT+TGG + tig0047679:893-912 Msa1466590:intron 15.0%
! CATAAGATTAGAAACTCTTA+AGG + tig0047679:363-382 Msa1466590:five_prime_UTR 25.0%
!!! TAGATTCTCATTTTGAGTTT+TGG + tig0047679:591-610 Msa1466590:five_prime_UTR 25.0%
!!! GAGTTTGTTTTGTGATTTTT+AGG + tig0047679:915-934 Msa1466590:intron 25.0%
CAGAATGAAAGAAAGAATTG+AGG - tig0047679:439-458 Msa1466590:intergenic 30.0%
! AGAATCTAAAAAAGTGCGTT+TGG - tig0047679:579-598 Msa1466590:intergenic 30.0%
CTGTCGAATCATATTCAAAA+CGG + tig0047679:636-655 Msa1466590:CDS 30.0%
!!! GGAACAGGTTTAGTTTTTTT+TGG + tig0047679:815-834 Msa1466590:intron 30.0%
! CGTTTGGATTAAGTTGTGTT+TGG - tig0047679:563-582 Msa1466590:intergenic 35.0%
TTCATAGTCAAGACTCTCAA+CGG - tig0047679:769-788 Msa1466590:intergenic 35.0%
! TGAGAATTTTGCTTATCGAG+AGG + tig0047679:794-813 Msa1466590:CDS 35.0%
!! ATTTTTAGGCGCATAGAAGA+AGG + tig0047679:929-948 Msa1466590:intron 35.0%
!! GATCGTTTTTCTTGTTGACG+TGG - tig0047679:391-410 Msa1466590:intergenic 40.0%
ATTGAGGTTCCAAACATGTG+CGG - tig0047679:423-442 Msa1466590:intergenic 40.0%
AAAGGTGATTCATCGGAGTT+CGG - tig0047679:696-715 Msa1466590:intergenic 40.0%
GGGATTCTTAAGAAGAACGA+TGG + tig0047679:738-757 Msa1466590:CDS 40.0%
! TTTTGCTTATCGAGAGGAAC+AGG + tig0047679:800-819 Msa1466590:CDS 40.0%
TTGAGGTTCCAAACATGTGC+GGG - tig0047679:422-441 Msa1466590:intergenic 45.0%
!! ACACTGTTAGTGGTGGATAG+AGG + tig0047679:505-524 Msa1466590:five_prime_UTR 45.0%
GAGGACGAAAGGTGATTCAT+CGG - tig0047679:703-722 Msa1466590:intergenic 45.0%
GCTGTATGTGAGTTACTTGC+TGG + tig0047679:954-973 Msa1466590:CDS 45.0%
! GTATGTGAGTTACTTGCTGG+TGG + tig0047679:957-976 Msa1466590:CDS 45.0%
! GTTACTTGCTGGTGGTGAAA+TGG + tig0047679:965-984 Msa1466590:CDS 45.0%
TTTCACCACCCGCACATGTT+TGG + tig0047679:411-430 Msa1466590:five_prime_UTR 50.0%
CGAACGAACGACACTGTTAG+TGG + tig0047679:495-514 Msa1466590:five_prime_UTR 50.0%
ACGAACGACACTGTTAGTGG+TGG + tig0047679:498-517 Msa1466590:five_prime_UTR 50.0%
TGATTCGACAGCATCGTCGT+TGG - tig0047679:627-646 Msa1466590:intergenic 50.0%
CCTTTCGTCCTCGATGATGA+CGG + tig0047679:711-730 Msa1466590:CDS 50.0%
AGGTTCCAAACATGTGCGGG+TGG - tig0047679:419-438 Msa1466590:intergenic 55.0%
CAGCATCGTCGTTGGTCGAT+CGG - tig0047679:619-638 Msa1466590:intergenic 55.0%
TCGAATCTGCGAGACTCGCT+TGG + tig0047679:661-680 Msa1466590:CDS 55.0%
CCGTCATCATCGAGGACGAA+AGG - tig0047679:714-733 Msa1466590:intergenic 55.0%
!! TCCTCGATGATGACGGCGTT+GGG + tig0047679:718-737 Msa1466590:CDS 55.0%
CTGCGAGACTCGCTTGGTCA+CGG + tig0047679:667-686 Msa1466590:CDS 60.0%
!! GTCCTCGATGATGACGGCGT+TGG + tig0047679:717-736 Msa1466590:CDS 60.0%
TCCCAACGCCGTCATCATCG+AGG - tig0047679:722-741 Msa1466590:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0047679 gene 358 999 358 ID=Msa1466590;Name=Msa1466590
tig0047679 mRNA 358 999 358 ID=Msa1466590-mRNA-1;Parent=Msa1466590;Name=Msa1466590-mRNA-1;_AED=0.00;_eAED=0.00;_QI=275|1|0.5|1|1|1|2|0|84
tig0047679 exon 358 821 358 ID=Msa1466590-mRNA-1:exon:25101;Parent=Msa1466590-mRNA-1
tig0047679 exon 937 999 937 ID=Msa1466590-mRNA-1:exon:25102;Parent=Msa1466590-mRNA-1
tig0047679 five_prime_UTR 358 632 358 ID=Msa1466590-mRNA-1:five_prime_utr;Parent=Msa1466590-mRNA-1
tig0047679 CDS 633 821 633 ID=Msa1466590-mRNA-1:cds;Parent=Msa1466590-mRNA-1
tig0047679 CDS 937 999 937 ID=Msa1466590-mRNA-1:cds;Parent=Msa1466590-mRNA-1
Gene Sequence

>Msa1466590

ATGCTGTCGAATCATATTCAAAACGGCATCGAATCTGCGAGACTCGCTTGGTCACGGATTCCGAACTCCGATGAATCACCTTTCGTCCTCGATGATGACGGCGTTGGGATTCTTAAGAAGAACGATGGAAGCGCCGTTGAGAGTCTTGACTATGAAGTCATTGAGAATTTTGCTTATCGAGAGGAACAGGCGCATAGAAGAAGGAAGCTGTATGTGAGTTACTTGCTGGTGGTGAAATGGTTCTTTGCATTG

Protein sequence

>Msa1466590

MLSNHIQNGIESARLAWSRIPNSDESPFVLDDDGVGILKKNDGSAVESLDYEVIENFAYREEQAHRRRKLYVSYLLVVKWFFAL