AlfalfaGEDB | Msa0118960

M. sativa cultivar zhongmu-4 / Msa0118960

KEGG: K07024
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016757
PFAM: PF05116.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0118960	sp\|Q9SJ66\|SPP2_ARATH	77.848	316	69	1	1	316	1	315	0.0	510

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0118960	Q9FQ10	98.738	317	4	0	1	317	1	317	0.0	643

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0023190	Msa0118960	0.806534	7.852774e-50	-8.615850e-47
Msa0035090	Msa0118960	0.819818	9.509636e-53	-8.615850e-47
Msa0046670	Msa0118960	0.803090	4.114623e-49	-8.615850e-47
Msa0071020	Msa0118960	0.820878	5.436098e-53	-8.615850e-47
Msa0083570	Msa0118960	0.808117	3.626104e-50	-8.615850e-47
Msa0086700	Msa0118960	0.811428	7.035093e-51	-8.615850e-47
Msa0092750	Msa0118960	0.802376	5.777288e-49	-8.615850e-47
Msa0093830	Msa0118960	0.800255	1.570351e-48	-8.615850e-47
Msa0118960	Msa0128840	0.815709	8.046542e-52	-8.615850e-47
Msa0118960	Msa0130120	0.823617	1.257907e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0118960	MtrunA17_Chr1g0176471	98.738	317	4	0	1	317	1	317	0.0	643
Msa0118960	MtrunA17_Chr1g0170411	68.889	315	96	1	1	315	1	313	3.02e-160	454

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0118960	AT2G35840.1	77.848	316	69	1	1	316	1	315	0.0	510
Msa0118960	AT2G35840.3	77.848	316	69	1	1	316	1	315	0.0	510
Msa0118960	AT2G35840.2	77.848	316	69	1	1	316	1	315	0.0	510
Msa0118960	AT2G35840.4	77.848	316	69	1	1	316	1	315	0.0	510
Msa0118960	AT1G51420.1	68.454	317	98	2	1	316	1	316	1.27e-158	450
Msa0118960	AT1G51420.3	68.454	317	98	2	1	316	2	317	1.28e-158	450
Msa0118960	AT1G51420.4	68.454	317	98	2	1	316	2	317	1.28e-158	450
Msa0118960	AT1G51420.2	68.454	317	98	2	1	316	2	317	1.28e-158	450
Msa0118960	AT3G52340.2	63.323	319	115	2	1	318	1	318	1.21e-148	424
Msa0118960	AT3G52340.7	63.323	319	115	2	1	318	1	318	1.21e-148	424

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Find 98 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCGATGGTTCCCGATGA+TGG	0.491125	1_3:-42243367	Msa0118960:CDS
AAGAAGGGTGGTGAGTGGTG+AGG	0.622953	1_3:+42244456	None:intergenic
AAGAGATATCAATCATACAG+TGG	0.672174	1_3:+42242242	None:intergenic
AAGGAATGGGAGAAGAAGAA+GGG	0.492050	1_3:+42244441	None:intergenic
AAGTTAGGGATTGTACATCA+TGG	0.577235	1_3:-42243883	Msa0118960:exon
AATCGATGGTTCCCGATGAT+GGG	0.556521	1_3:-42243366	Msa0118960:CDS
AATGTCTGAAACATCTCTTG+GGG	0.696343	1_3:+42242392	None:intergenic
ACACACATTAGTAGAAGAAG+CGG	0.567175	1_3:-42244541	Msa0118960:five_prime_UTR
ACAGACATGATGGTTATATC+TGG	0.375892	1_3:+42243412	None:intergenic
ACATCTCTTGGGGACAGGTT+TGG	0.339450	1_3:+42242402	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTAAATTGGCGAGATTAAA+TGG	+	chr1_3:42242707-42242726	None:intergenic	30.0%
AAGTACAATTGGGACTAAAT+TGG	-	chr1_3:42242768-42242787	Msa0118960:CDS	30.0%
ATTTCTGTTTAGGAAGAGAA+AGG	-	chr1_3:42242840-42242859	Msa0118960:CDS	30.0%
TTTCTGTTTAGGAAGAGAAA+GGG	-	chr1_3:42242841-42242860	Msa0118960:CDS	30.0%
GATTACTTATGGAAAATCGA+TGG	-	chr1_3:42243447-42243466	Msa0118960:CDS	30.0%
TTCAAGAATTGAACCAGAAA+TGG	-	chr1_3:42243488-42243507	Msa0118960:CDS	30.0%
TCAAGAATTGAACCAGAAAT+GGG	-	chr1_3:42243489-42243508	Msa0118960:CDS	30.0%
TTTGTCTGTTAAGTGAAAAG+TGG	-	chr1_3:42244510-42244529	Msa0118960:five_prime_UTR	30.0%
ACTAAATTGGCGAGATTAAA+TGG	+	chr1_3:42242707-42242726	None:intergenic	30.0%
AAGTACAATTGGGACTAAAT+TGG	-	chr1_3:42242768-42242787	Msa0118960:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	42242254	42244595	42242254	ID=Msa0118960;Name=Msa0118960
chr1_3	mRNA	42242254	42244595	42242254	ID=Msa0118960-mRNA-1;Parent=Msa0118960;Name=Msa0118960-mRNA-1;_AED=0.11;_eAED=0.11;_QI=197\|1\|0.83\|1\|0.8\|0.66\|6\|0\|318
chr1_3	exon	42242254	42242553	42242254	ID=Msa0118960-mRNA-1:exon:12781;Parent=Msa0118960-mRNA-1
chr1_3	exon	42242680	42242874	42242680	ID=Msa0118960-mRNA-1:exon:12780;Parent=Msa0118960-mRNA-1
chr1_3	exon	42243015	42243116	42243015	ID=Msa0118960-mRNA-1:exon:12779;Parent=Msa0118960-mRNA-1
chr1_3	exon	42243285	42243584	42243285	ID=Msa0118960-mRNA-1:exon:12778;Parent=Msa0118960-mRNA-1
chr1_3	exon	42243827	42243909	42243827	ID=Msa0118960-mRNA-1:exon:12777;Parent=Msa0118960-mRNA-1
chr1_3	exon	42244422	42244595	42244422	ID=Msa0118960-mRNA-1:exon:12776;Parent=Msa0118960-mRNA-1
chr1_3	five_prime_UTR	42244422	42244595	42244422	ID=Msa0118960-mRNA-1:five_prime_utr;Parent=Msa0118960-mRNA-1
chr1_3	five_prime_UTR	42243887	42243909	42243887	ID=Msa0118960-mRNA-1:five_prime_utr;Parent=Msa0118960-mRNA-1
chr1_3	CDS	42243827	42243886	42243827	ID=Msa0118960-mRNA-1:cds;Parent=Msa0118960-mRNA-1
chr1_3	CDS	42243285	42243584	42243285	ID=Msa0118960-mRNA-1:cds;Parent=Msa0118960-mRNA-1
chr1_3	CDS	42243015	42243116	42243015	ID=Msa0118960-mRNA-1:cds;Parent=Msa0118960-mRNA-1
chr1_3	CDS	42242680	42242874	42242680	ID=Msa0118960-mRNA-1:cds;Parent=Msa0118960-mRNA-1
chr1_3	CDS	42242254	42242553	42242254	ID=Msa0118960-mRNA-1:cds;Parent=Msa0118960-mRNA-1

Gene Sequence

>Msa0118960

ATGGATAGACTCAAATCTTCTGCGCGGCTCATGATTGTTTCCGATCTCGATCACACAATGGTTGATCACCATGACGCAGAGAATAGTTCGCTTTTGAGGTTCAATGCCCTTTGGGAAGCCTCATATCGCCACGATTCTTTGCTGGTGTTCTCAACTGGCAGGTCGCCTTTACTCTACAAACAACTGAGGAAAGAGAAGCCTATGATAACTCCAGATATAACCATCATGTCTGTGGGAACTGAGATTACTTATGGAAAATCGATGGTTCCCGATGATGGGTGGGTTCAAGAATTGAACCAGAAATGGGACAAGGACATTGTCATTGAGGAAGCAAGCAAGTTTCCTGAACTTAAGCCTCAGGCAGAAACAGAACAACGGGCACACAAAGTTAGCTTTTATGTAGAGAAAGACAATGCTAAGCAAGTGACAGAGGCTCTTTCTAAGATTTTGGAACAGCGTGGGTTGGATGTTAAAATAATATATAGCGGAGGAGTTGACATGGACATATTACCACAAGGTGCTGGCAAAGGTCAAGCTCTTGCCTATTTGCTTAAAAAGTTTGAGACTGAGGGAAAGCTACCTGGTAATACTCTAGTTTGTGGTGACTCCGGAAATGATGCCGAGCTATTCAGCATTCCAGGAGTTTATGGTGTCATGGTAAGCAACGCCCAAGAGGAATTACTGCAGTGGCATGCCGAAAATGCAAAAGATAACCCTAAGATTCTGCATGCCTCAGAGCGCTGTGCATCTGGGATTATACAAGCCATAGGTCATTTTAACTTGGGCCCAAACCTGTCCCCAAGAGATGTTTCAGACATTGGACAAGAACAGAGTGTTGAAAATGTGTCTGCAGTTCAAGAAATAGTGAACTTTTGTCTGTTAAGTGAAAAGTGGAGACGTGCAGAAGTTGAGAACTCGGAGCTGTTTATTGCTGCTATAAAGGCATCCACTGTATGA

Protein sequence

>Msa0118960

MDRLKSSARLMIVSDLDHTMVDHHDAENSSLLRFNALWEASYRHDSLLVFSTGRSPLLYKQLRKEKPMITPDITIMSVGTEITYGKSMVPDDGWVQELNQKWDKDIVIEEASKFPELKPQAETEQRAHKVSFYVEKDNAKQVTEALSKILEQRGLDVKIIYSGGVDMDILPQGAGKGQALAYLLKKFETEGKLPGNTLVCGDSGNDAELFSIPGVYGVMVSNAQEELLQWHAENAKDNPKILHASERCASGIIQAIGHFNLGPNLSPRDVSDIGQEQSVENVSAVQEIVNFCLLSEKWRRAEVENSELFIAAIKASTV*