AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0465480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0465480 MtrunA17_Chr3g0094731 87.097 31 4 0 64 94 2 32 1.98e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 138 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACTTGGTTTGCCCTTAT+TGG 0.113834 3_3:+65889092 Msa0465480:three_prime_UTR
CCAAAGATTTGATGCTTTAA+AGG 0.137745 3_3:+65888190 Msa0465480:exon
CCATTAGTTCTGATTCTATT+TGG 0.139282 3_3:+65888711 Msa0465480:three_prime_UTR
CATAACTGGTTTATATGTTT+TGG 0.181603 3_3:+65888661 Msa0465480:three_prime_UTR
TCAATCTCTGCCTCCATTTC+CGG 0.200373 3_3:+65886547 Msa0465480:CDS
GATGTAAGTCTTCTCGTTTC+AGG 0.212723 3_3:+65888560 Msa0465480:three_prime_UTR
CCTTTAAAGCATCAAATCTT+TGG 0.214941 3_3:-65888190 None:intergenic
TTTCTTTCTTCAAATCATTT+AGG 0.225821 3_3:-65889413 None:intergenic
TTTGCCCTTATTGGCTTATA+AGG 0.252639 3_3:+65889101 Msa0465480:three_prime_UTR
TTATTGGCTTATAAGGATTC+AGG 0.266337 3_3:+65889108 Msa0465480:three_prime_UTR
TGAATCCTTATAAGCCAATA+AGG 0.269795 3_3:-65889106 None:intergenic
AAAGCATTTCTTGAGTAAAA+AGG 0.276516 3_3:-65888595 None:intergenic
CTTTGGGTATATGCAATTAA+AGG 0.278656 3_3:-65888173 None:intergenic
CCTTACAAGTTACAACAAAT+AGG 0.282293 3_3:-65887170 None:intergenic
ATGGTTATTTGTTGAGAAAT+TGG 0.286770 3_3:+65889205 Msa0465480:three_prime_UTR
TGTGCTGCTACTTAGTTTGT+TGG 0.298882 3_3:+65889063 Msa0465480:three_prime_UTR
GCGGCGGGCGGCACCGGAAA+TGG 0.300903 3_3:-65886560 None:intergenic
CTCAGATCGTTGTTAGGTTA+AGG 0.312433 3_3:+65886624 Msa0465480:CDS
TTCTTGCTGTTCAAAGTTAG+AGG 0.315918 3_3:+65889303 Msa0465480:three_prime_UTR
TTGCTCTTGTTGTCTTGGTC+TGG 0.321036 3_3:+65888489 Msa0465480:three_prime_UTR
AGAGAAGCGGCGGGCGGCAC+CGG 0.324219 3_3:-65886566 None:intergenic
CATCATGTTATTCTCGTTGC+AGG 0.326129 3_3:+65888913 Msa0465480:three_prime_UTR
CATTGTTGCTCTTGTTGTCT+TGG 0.328685 3_3:+65888484 Msa0465480:three_prime_UTR
CATTAGTTCTGATTCTATTT+GGG 0.331805 3_3:+65888712 Msa0465480:three_prime_UTR
CTTACAAGTTACAACAAATA+GGG 0.340146 3_3:-65887169 None:intergenic
TTGATGCTTTAAAGGACAAT+TGG 0.341171 3_3:+65888198 Msa0465480:exon
CTTTAAAGCATCAAATCTTT+GGG 0.343852 3_3:-65888189 None:intergenic
TAAGGTTCGGTCGCTTGCTT+TGG 0.353148 3_3:-65887015 None:intergenic
CCTATTTGTTGTAACTTGTA+AGG 0.353633 3_3:+65887170 Msa0465480:CDS
ATTGGAGTGCCCTATGGTTT+TGG 0.356794 3_3:+65889223 Msa0465480:three_prime_UTR
TCTGATTCTGCCGTTGCATC+TGG 0.359311 3_3:+65888239 Msa0465480:three_prime_UTR
CCCTCTCTCAGATCGTTGTT+AGG 0.372410 3_3:+65886618 Msa0465480:CDS
TATTGTAATGGATGTCAAAA+TGG 0.373197 3_3:+65888274 Msa0465480:three_prime_UTR
ACCGATGAAACCTTTCTTGA+AGG 0.377730 3_3:+65888380 Msa0465480:three_prime_UTR
TGAATGCTAGTGTTGTAAAA+AGG 0.384855 3_3:+65888411 Msa0465480:three_prime_UTR
CGCCGGCGGTGGTGTGGTGG+TGG 0.392155 3_3:-65887041 None:intergenic
TTGAGGAGTAACAGATAAGA+GGG 0.401120 3_3:-65886528 None:intergenic
ATTGAGGAGTAACAGATAAG+AGG 0.403291 3_3:-65886529 None:intergenic
CATGACCATTCCTTCAAGAA+AGG 0.407072 3_3:-65888390 None:intergenic
ATGTAACCAGTGGAGCAAAA+AGG 0.410867 3_3:-65888940 None:intergenic
TTTGTTGGTCTTTGTCAACT+TGG 0.414907 3_3:+65889078 Msa0465480:three_prime_UTR
TAATGGACCTCGTAAGAGTT+TGG 0.421250 3_3:-65888694 None:intergenic
GCAAACACATGTAGTCTTGA+TGG 0.423869 3_3:-65888890 None:intergenic
AACTAAGTAGCAGCACAAAT+AGG 0.429506 3_3:-65889057 None:intergenic
CCAAATAGAATCAGAACTAA+TGG 0.429636 3_3:-65888711 None:intergenic
CTGAATAATCATGCAGGAAA+AGG 0.430468 3_3:-65888763 None:intergenic
CCGCTTCTCTCTCCCTCTTC+CGG 0.430738 3_3:+65886579 Msa0465480:CDS
GTGGTGTGGTGGTGGATGTA+AGG 0.435643 3_3:-65887033 None:intergenic
TTGATCTAAATTTAGACGAT+TGG 0.435971 3_3:+65888104 Msa0465480:CDS
TTCGGTCGCTTGCTTTGGTC+GGG 0.441888 3_3:-65887010 None:intergenic
GTTCGGTCGCTTGCTTTGGT+CGG 0.444976 3_3:-65887011 None:intergenic
TGATGCTTTAAAGGACAATT+GGG 0.445203 3_3:+65888199 Msa0465480:exon
AATTAAATAAGTTATTGTAA+TGG 0.447242 3_3:+65888262 Msa0465480:three_prime_UTR
AGAAGGAGATGAAACTGAAT+AGG 0.450844 3_3:-65886946 None:intergenic
TGGTCGCGGCTCGCCGGCGG+TGG 0.462159 3_3:-65887052 None:intergenic
GCAGGGTTCTTCAAAGCCCC+TGG 0.463363 3_3:-65888803 None:intergenic
AATGCATAGATATTCAGAAA+TGG 0.466377 3_3:-65888465 None:intergenic
AGCATTTACAGATATACAAC+TGG 0.476097 3_3:-65888831 None:intergenic
CAGTGTGTACACAAAACTTA+AGG 0.477379 3_3:-65889026 None:intergenic
CTTCTCCGCCTCGAGCCAAA+TGG 0.488538 3_3:+65888309 Msa0465480:three_prime_UTR
GAATCCACTGTCAAAGCCCC+AGG 0.489514 3_3:+65888785 Msa0465480:three_prime_UTR
CGTTCTCAGATGAAAGAGAA+AGG 0.497516 3_3:-65886971 None:intergenic
GCGGCTCGCCGGCGGTGGTG+TGG 0.498620 3_3:-65887047 None:intergenic
GAATATAAGTTTGAGATCAA+TGG 0.499009 3_3:+65889130 Msa0465480:three_prime_UTR
AGAGAGTGGTCGCGGCTCGC+CGG 0.499272 3_3:-65887058 None:intergenic
GCCTATGGTGGTATGCAACC+TGG 0.501903 3_3:+65888341 Msa0465480:three_prime_UTR
GAACATGCTAAAGGTGATGC+TGG 0.502172 3_3:+65888433 Msa0465480:three_prime_UTR
GAATCCTTATAAGCCAATAA+GGG 0.505222 3_3:-65889105 None:intergenic
GATATACAACTGGTTAATGC+AGG 0.505761 3_3:-65888821 None:intergenic
AGCCCCTGGGGCTTTGACAG+TGG 0.507837 3_3:-65888789 None:intergenic
CAGGGTTCTTCAAAGCCCCT+GGG 0.509672 3_3:-65888802 None:intergenic
GATGAAAGAGAAAGGGAAGA+AGG 0.513109 3_3:-65886963 None:intergenic
GGAGTAGGATGCGGCGGTGA+CGG 0.516011 3_3:-65887090 None:intergenic
ACAAAGCTTAGCCTCGTCCC+AGG 0.517211 3_3:-65889155 None:intergenic
ACAGAGGTAATCGCCGGAAG+AGG 0.519134 3_3:-65886592 None:intergenic
TGAAACCTTTCTTGAAGGAA+TGG 0.519707 3_3:+65888385 Msa0465480:three_prime_UTR
GCTCGCCGGCGGTGGTGTGG+TGG 0.523397 3_3:-65887044 None:intergenic
TCCTTCAAGAAAGGTTTCAT+CGG 0.524359 3_3:-65888381 None:intergenic
GGAAATGGAGGCAGAGATTG+AGG 0.527436 3_3:-65886545 None:intergenic
TATACTTCCACCAAAACCAT+AGG 0.528424 3_3:-65889233 None:intergenic
TGAAGAATCGAAACAGAAGA+TGG 0.530010 3_3:-65887111 None:intergenic
TAGGATGCGGCGGTGACGGA+TGG 0.530865 3_3:-65887086 None:intergenic
AAGAGGGAGAGAGAAGCGGC+GGG 0.533281 3_3:-65886575 None:intergenic
CTCGTAAGAGTTTGGTAGAT+AGG 0.533723 3_3:-65888686 None:intergenic
GCGGGCGGCACCGGAAATGG+AGG 0.536841 3_3:-65886557 None:intergenic
GTATGATTGTCATCATATCC+AGG 0.540147 3_3:-65888359 None:intergenic
AGATATTGAGGGCATAATGA+AGG 0.541095 3_3:-65888631 None:intergenic
TGCTGTTCAAAGTTAGAGGA+GGG 0.542437 3_3:+65889307 Msa0465480:three_prime_UTR
AAATGCTTCTGTAGTCGCCT+CGG 0.545200 3_3:+65888847 Msa0465480:three_prime_UTR
GAAGAGGGAGAGAGAAGCGG+CGG 0.547894 3_3:-65886576 None:intergenic
TGAGAAATTGGAGTGCCCTA+TGG 0.553196 3_3:+65889217 Msa0465480:three_prime_UTR
ATCCACCACCACACCACCGC+CGG 0.554325 3_3:+65887039 Msa0465480:CDS
TGCGGCGGTGACGGATGGAA+AGG 0.554826 3_3:-65887081 None:intergenic
AAATGGAGAAAAGTAGCCTA+TGG 0.557172 3_3:+65888326 Msa0465480:three_prime_UTR
TTAGGATTAAGTTGATGGGA+AGG 0.560136 3_3:+65889280 Msa0465480:three_prime_UTR
TGGATTCTGAATAATCATGC+AGG 0.568914 3_3:-65888769 None:intergenic
CAGAGGTAATCGCCGGAAGA+GGG 0.570916 3_3:-65886591 None:intergenic
GTGGTGGTGGATGTAAGGTT+CGG 0.573274 3_3:-65887028 None:intergenic
GTTCTCAGATGAAAGAGAAA+GGG 0.575776 3_3:-65886970 None:intergenic
GGGTATATGCAATTAAAGGT+AGG 0.576748 3_3:-65888169 None:intergenic
TGCAATTAAAGGTAGGAGGT+AGG 0.581209 3_3:-65888162 None:intergenic
GAGTGGTCGCGGCTCGCCGG+CGG 0.582631 3_3:-65887055 None:intergenic
AGGGAGAGAGAAGCGGCGGG+CGG 0.584521 3_3:-65886572 None:intergenic
AAAGCATATGTGTAAGCAGA+AGG 0.584702 3_3:-65888981 None:intergenic
GATCAATGGACCCTGGGACG+AGG 0.585309 3_3:+65889144 Msa0465480:three_prime_UTR
TTAATACAGTATGTAACCAG+TGG 0.586025 3_3:-65888950 None:intergenic
TTGCTGTTCAAAGTTAGAGG+AGG 0.587504 3_3:+65889306 Msa0465480:three_prime_UTR
TTTAGACGATTGGAAGACAA+TGG 0.588598 3_3:+65888114 Msa0465480:CDS
TGGAAAGGAGAGAGTGGTCG+CGG 0.593298 3_3:-65887066 None:intergenic
TAATTAAGATTGAAAACATG+AGG 0.595355 3_3:-65889442 None:intergenic
TTATTTAATTCCAGATGCAA+CGG 0.595595 3_3:-65888249 None:intergenic
CCGGAAGAGGGAGAGAGAAG+CGG 0.598353 3_3:-65886579 None:intergenic
ACAGAAGATGGAGTAGGATG+CGG 0.598733 3_3:-65887099 None:intergenic
GTTTGAGATCAATGGACCCT+GGG 0.600084 3_3:+65889138 Msa0465480:three_prime_UTR
AGTTTGAGATCAATGGACCC+TGG 0.600417 3_3:+65889137 Msa0465480:three_prime_UTR
TATATGCAATTAAAGGTAGG+AGG 0.601265 3_3:-65888166 None:intergenic
TCCAGGTTGCATACCACCAT+AGG 0.605048 3_3:-65888342 None:intergenic
CTTAATCCTAAAACTAGACC+AGG 0.605573 3_3:-65889268 None:intergenic
ACCTAACAACGATCTGAGAG+AGG 0.609167 3_3:-65886619 None:intergenic
ATACTTCCACCAAAACCATA+GGG 0.611580 3_3:-65889232 None:intergenic
AATCCACTGTCAAAGCCCCA+GGG 0.616730 3_3:+65888786 Msa0465480:three_prime_UTR
GGGAACACAGAGGTAATCGC+CGG 0.617254 3_3:-65886598 None:intergenic
TTTCTCCATTTGGCTCGAGG+CGG 0.617404 3_3:-65888314 None:intergenic
CAAAGCTTAGCCTCGTCCCA+GGG 0.620911 3_3:-65889154 None:intergenic
CTGCAACGAGAATAACATGA+TGG 0.622923 3_3:-65888912 None:intergenic
CCTAACAACGATCTGAGAGA+GGG 0.640920 3_3:-65886618 None:intergenic
GACGGATGGAAAGGAGAGAG+TGG 0.640972 3_3:-65887072 None:intergenic
ATCGAAACAGAAGATGGAGT+AGG 0.642445 3_3:-65887105 None:intergenic
AGTATAATTTACTCACTACC+TGG 0.643620 3_3:+65889250 Msa0465480:three_prime_UTR
CTATCTACCAAACTCTTACG+AGG 0.647536 3_3:+65888687 Msa0465480:three_prime_UTR
TGGAGAAAAGTAGCCTATGG+TGG 0.650401 3_3:+65888329 Msa0465480:three_prime_UTR
TTTAGGTAAATATACACCCA+TGG 0.653298 3_3:-65889396 None:intergenic
GAAGATGGAGTAGGATGCGG+CGG 0.656692 3_3:-65887096 None:intergenic
ATATACAACTGGTTAATGCA+GGG 0.660347 3_3:-65888820 None:intergenic
AGGGTTCTTCAAAGCCCCTG+GGG 0.663398 3_3:-65888801 None:intergenic
TACAGACTGAAATCAGAACA+TGG 0.698765 3_3:+65889186 Msa0465480:three_prime_UTR
ATCCACTGTCAAAGCCCCAG+GGG 0.705854 3_3:+65888787 Msa0465480:three_prime_UTR
ATCTGAGAGAGGGAACACAG+AGG 0.785629 3_3:-65886608 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTAAAATTACATAAAA+AGG - chr3_3:65888066-65888085 None:intergenic 10.0%
!! AATTAAATAAGTTATTGTAA+TGG + chr3_3:65888262-65888281 Msa0465480:three_prime_UTR 10.0%
!! ATACAAACAAATAAATAAAG+AGG - chr3_3:65886821-65886840 None:intergenic 15.0%
!! AATTAACAAAATATTATCAC+AGG - chr3_3:65887429-65887448 None:intergenic 15.0%
!!! TATATTTTAGTGTATTTTGA+TGG + chr3_3:65887587-65887606 Msa0465480:intron 15.0%
!!! TTTAGAATTTTATGTAATGA+AGG + chr3_3:65889356-65889375 Msa0465480:three_prime_UTR 15.0%
!! AAATGAATTAAAGGAGAAAA+GGG - chr3_3:65886737-65886756 None:intergenic 20.0%
!! AAAATGAATTAAAGGAGAAA+AGG - chr3_3:65886738-65886757 None:intergenic 20.0%
!! ACGAAAAGAAAATGAATTAA+AGG - chr3_3:65886746-65886765 None:intergenic 20.0%
!! AGTAAAATTACATAAAAAGG+AGG - chr3_3:65888063-65888082 None:intergenic 20.0%
!!! TAATTTTACTTTTCACAGTT+CGG + chr3_3:65888073-65888092 Msa0465480:intron 20.0%
!! TATGAAAAATGAGATATTGA+GGG - chr3_3:65888645-65888664 None:intergenic 20.0%
!! TTATGAAAAATGAGATATTG+AGG - chr3_3:65888646-65888665 None:intergenic 20.0%
!!! AATATCTCATTTTTCATAAC+TGG + chr3_3:65888647-65888666 Msa0465480:three_prime_UTR 20.0%
!! TTTCTTTCTTCAAATCATTT+AGG - chr3_3:65889416-65889435 None:intergenic 20.0%
!! TAATTAAGATTGAAAACATG+AGG - chr3_3:65889445-65889464 None:intergenic 20.0%
!!! AAATGTTATACTGTTTTTAC+AGG - chr3_3:65889489-65889508 None:intergenic 20.0%
!! TCACAAGTTGGAAATTTATT+AGG + chr3_3:65886867-65886886 Msa0465480:intron 25.0%
!!! ATTGTTGCTATTGTTTTGTA+TGG + chr3_3:65886892-65886911 Msa0465480:intron 25.0%
!!! TTGTTGCTATTGTTTTGTAT+GGG + chr3_3:65886893-65886912 Msa0465480:intron 25.0%
! GAATTCTGCAAATTAATGAA+GGG - chr3_3:65886927-65886946 None:intergenic 25.0%
! CTTACAAGTTACAACAAATA+GGG - chr3_3:65887172-65887191 None:intergenic 25.0%
! CAAATCAGAATCATAATTGA+AGG - chr3_3:65887219-65887238 None:intergenic 25.0%
!!! TTTTAGTGTATTTTGATGGT+CGG + chr3_3:65887591-65887610 Msa0465480:intron 25.0%
! AAAACTAAGCTAATCCAAAT+GGG + chr3_3:65887686-65887705 Msa0465480:intron 25.0%
! TGTAAAGATCAAAACAAAGT+TGG - chr3_3:65887935-65887954 None:intergenic 25.0%
! TTGATCTAAATTTAGACGAT+TGG + chr3_3:65888104-65888123 Msa0465480:CDS 25.0%
!!! CTTTAAAGCATCAAATCTTT+GGG - chr3_3:65888192-65888211 None:intergenic 25.0%
! TTATTTAATTCCAGATGCAA+CGG - chr3_3:65888252-65888271 None:intergenic 25.0%
!!! TATTGTAATGGATGTCAAAA+TGG + chr3_3:65888274-65888293 Msa0465480:three_prime_UTR 25.0%
! AATGCATAGATATTCAGAAA+TGG - chr3_3:65888468-65888487 None:intergenic 25.0%
!! AAAGCATTTCTTGAGTAAAA+AGG - chr3_3:65888598-65888617 None:intergenic 25.0%
!!! CATAACTGGTTTATATGTTT+TGG + chr3_3:65888661-65888680 Msa0465480:three_prime_UTR 25.0%
!!! CATTAGTTCTGATTCTATTT+GGG + chr3_3:65888712-65888731 Msa0465480:three_prime_UTR 25.0%
!!! ATGCTTTTCTATTAGTTTGA+TGG + chr3_3:65888997-65889016 Msa0465480:three_prime_UTR 25.0%
!! GAATATAAGTTTGAGATCAA+TGG + chr3_3:65889130-65889149 Msa0465480:three_prime_UTR 25.0%
!! ATGGTTATTTGTTGAGAAAT+TGG + chr3_3:65889205-65889224 Msa0465480:three_prime_UTR 25.0%
!!! TAGTTTTAGGATTAAGTTGA+TGG + chr3_3:65889275-65889294 Msa0465480:three_prime_UTR 25.0%
!!! AGTTTTAGGATTAAGTTGAT+GGG + chr3_3:65889276-65889295 Msa0465480:three_prime_UTR 25.0%
!! AATGAAGGTTTCACAATTTT+AGG + chr3_3:65889371-65889390 Msa0465480:three_prime_UTR 25.0%
! AAAAAAGTGGTATTGGAGAA+AGG - chr3_3:65886657-65886676 None:intergenic 30.0%
AATGCATGTTGCATTGAATT+TGG - chr3_3:65886696-65886715 None:intergenic 30.0%
TCAGTTTCCAAATTCAAGAA+CGG - chr3_3:65886795-65886814 None:intergenic 30.0%
TGTTTGTATGTGTTTACTCA+TGG + chr3_3:65886832-65886851 Msa0465480:intron 30.0%
GGAATTCTGCAAATTAATGA+AGG - chr3_3:65886928-65886947 None:intergenic 30.0%
CCTTACAAGTTACAACAAAT+AGG - chr3_3:65887173-65887192 None:intergenic 30.0%
!! CCTATTTGTTGTAACTTGTA+AGG + chr3_3:65887170-65887189 Msa0465480:CDS 30.0%
ATTACCCTAAAAACAACAGA+TGG - chr3_3:65887281-65887300 None:intergenic 30.0%
!!! GTTTTTAGGGTAATTTGTAG+CGG + chr3_3:65887287-65887306 Msa0465480:intron 30.0%
!!! TTTTTAGGGTAATTTGTAGC+GGG + chr3_3:65887288-65887307 Msa0465480:intron 30.0%
! GGATCATTTTCTTCAATTCT+TGG - chr3_3:65887532-65887551 None:intergenic 30.0%
AGAACTCTATTAACCTTTGA+AGG - chr3_3:65887559-65887578 None:intergenic 30.0%
TAGCTTATGCAAATAAACAG+TGG - chr3_3:65887652-65887671 None:intergenic 30.0%
CAAAACTAAGCTAATCCAAA+TGG + chr3_3:65887685-65887704 Msa0465480:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG - chr3_3:65887712-65887731 None:intergenic 30.0%
ATTTGAGCTTATATCTCTAC+TGG + chr3_3:65887726-65887745 Msa0465480:intron 30.0%
TATATGCAATTAAAGGTAGG+AGG - chr3_3:65888169-65888188 None:intergenic 30.0%
CTTTGGGTATATGCAATTAA+AGG - chr3_3:65888176-65888195 None:intergenic 30.0%
!! CCTTTAAAGCATCAAATCTT+TGG - chr3_3:65888193-65888212 None:intergenic 30.0%
CCAAAGATTTGATGCTTTAA+AGG + chr3_3:65888190-65888209 Msa0465480:exon 30.0%
!! TTGATGCTTTAAAGGACAAT+TGG + chr3_3:65888198-65888217 Msa0465480:exon 30.0%
!! TGATGCTTTAAAGGACAATT+GGG + chr3_3:65888199-65888218 Msa0465480:exon 30.0%
TGAATGCTAGTGTTGTAAAA+AGG + chr3_3:65888411-65888430 Msa0465480:three_prime_UTR 30.0%
TGTAAAAAGGAACATGCTAA+AGG + chr3_3:65888424-65888443 Msa0465480:three_prime_UTR 30.0%
CCAAATAGAATCAGAACTAA+TGG - chr3_3:65888714-65888733 None:intergenic 30.0%
!! CCATTAGTTCTGATTCTATT+TGG + chr3_3:65888711-65888730 Msa0465480:three_prime_UTR 30.0%
ATATACAACTGGTTAATGCA+GGG - chr3_3:65888823-65888842 None:intergenic 30.0%
AGCATTTACAGATATACAAC+TGG - chr3_3:65888834-65888853 None:intergenic 30.0%
TTAATACAGTATGTAACCAG+TGG - chr3_3:65888953-65888972 None:intergenic 30.0%
GAATCCTTATAAGCCAATAA+GGG - chr3_3:65889108-65889127 None:intergenic 30.0%
TGAATCCTTATAAGCCAATA+AGG - chr3_3:65889109-65889128 None:intergenic 30.0%
!! TTATTGGCTTATAAGGATTC+AGG + chr3_3:65889108-65889127 Msa0465480:three_prime_UTR 30.0%
AGTATAATTTACTCACTACC+TGG + chr3_3:65889250-65889269 Msa0465480:three_prime_UTR 30.0%
!! TTTCACAATTTTAGGTACCA+TGG + chr3_3:65889379-65889398 Msa0465480:three_prime_UTR 30.0%
!! TTCACAATTTTAGGTACCAT+GGG + chr3_3:65889380-65889399 Msa0465480:three_prime_UTR 30.0%
TTTAGGTAAATATACACCCA+TGG - chr3_3:65889399-65889418 None:intergenic 30.0%
TTGAGGAGTAACAGATAAGA+GGG - chr3_3:65886531-65886550 None:intergenic 35.0%
ATTGAGGAGTAACAGATAAG+AGG - chr3_3:65886532-65886551 None:intergenic 35.0%
AGCGACAAAAAAAGTGGTAT+TGG - chr3_3:65886664-65886683 None:intergenic 35.0%
ATGAACAGCGACAAAAAAAG+TGG - chr3_3:65886670-65886689 None:intergenic 35.0%
AATGTACCCGTTCTTGAATT+TGG + chr3_3:65886785-65886804 Msa0465480:intron 35.0%
CAGTTTCCAAATTCAAGAAC+GGG - chr3_3:65886794-65886813 None:intergenic 35.0%
AGAAGGAGATGAAACTGAAT+AGG - chr3_3:65886949-65886968 None:intergenic 35.0%
GTTCTCAGATGAAAGAGAAA+GGG - chr3_3:65886973-65886992 None:intergenic 35.0%
TGAAGAATCGAAACAGAAGA+TGG - chr3_3:65887114-65887133 None:intergenic 35.0%
!!! TCCTTCCATCTGTTGTTTTT+AGG + chr3_3:65887273-65887292 Msa0465480:intron 35.0%
!!! CCTTCCATCTGTTGTTTTTA+GGG + chr3_3:65887274-65887293 Msa0465480:intron 35.0%
!! CTCATGAACTTGTTAAGGTT+CGG - chr3_3:65887506-65887525 None:intergenic 35.0%
!! GGATTCTCATGAACTTGTTA+AGG - chr3_3:65887511-65887530 None:intergenic 35.0%
! TCTATTAACCTTTGAAGGAC+GGG - chr3_3:65887554-65887573 None:intergenic 35.0%
! CTCTATTAACCTTTGAAGGA+CGG - chr3_3:65887555-65887574 None:intergenic 35.0%
CTTATGCAAATAAACAGTGG+CGG - chr3_3:65887649-65887668 None:intergenic 35.0%
GCATATGCTATACACTATCT+TGG - chr3_3:65887814-65887833 None:intergenic 35.0%
TAGCATAAGCGCTTATGTAT+AGG - chr3_3:65887879-65887898 None:intergenic 35.0%
TTTAGACGATTGGAAGACAA+TGG + chr3_3:65888114-65888133 Msa0465480:CDS 35.0%
GGGTATATGCAATTAAAGGT+AGG - chr3_3:65888172-65888191 None:intergenic 35.0%
!!! ATAGGCTACTTTTCTCCATT+TGG - chr3_3:65888327-65888346 None:intergenic 35.0%
AAATGGAGAAAAGTAGCCTA+TGG + chr3_3:65888326-65888345 Msa0465480:three_prime_UTR 35.0%
GTATGATTGTCATCATATCC+AGG - chr3_3:65888362-65888381 None:intergenic 35.0%
TCCTTCAAGAAAGGTTTCAT+CGG - chr3_3:65888384-65888403 None:intergenic 35.0%
TGAAACCTTTCTTGAAGGAA+TGG + chr3_3:65888385-65888404 Msa0465480:three_prime_UTR 35.0%
! AGATATTGAGGGCATAATGA+AGG - chr3_3:65888634-65888653 None:intergenic 35.0%
CTGAATAATCATGCAGGAAA+AGG - chr3_3:65888766-65888785 None:intergenic 35.0%
TGGATTCTGAATAATCATGC+AGG - chr3_3:65888772-65888791 None:intergenic 35.0%
GATATACAACTGGTTAATGC+AGG - chr3_3:65888824-65888843 None:intergenic 35.0%
AAAGCATATGTGTAAGCAGA+AGG - chr3_3:65888984-65889003 None:intergenic 35.0%
CAGTGTGTACACAAAACTTA+AGG - chr3_3:65889029-65889048 None:intergenic 35.0%
AACTAAGTAGCAGCACAAAT+AGG - chr3_3:65889060-65889079 None:intergenic 35.0%
!! TTTGTTGGTCTTTGTCAACT+TGG + chr3_3:65889078-65889097 Msa0465480:three_prime_UTR 35.0%
!! TTTGCCCTTATTGGCTTATA+AGG + chr3_3:65889101-65889120 Msa0465480:three_prime_UTR 35.0%
TACAGACTGAAATCAGAACA+TGG + chr3_3:65889186-65889205 Msa0465480:three_prime_UTR 35.0%
ATACTTCCACCAAAACCATA+GGG - chr3_3:65889235-65889254 None:intergenic 35.0%
TATACTTCCACCAAAACCAT+AGG - chr3_3:65889236-65889255 None:intergenic 35.0%
CTTAATCCTAAAACTAGACC+AGG - chr3_3:65889271-65889290 None:intergenic 35.0%
!! TTAGGATTAAGTTGATGGGA+AGG + chr3_3:65889280-65889299 Msa0465480:three_prime_UTR 35.0%
!! TTCTTGCTGTTCAAAGTTAG+AGG + chr3_3:65889303-65889322 Msa0465480:three_prime_UTR 35.0%
CTCAGATCGTTGTTAGGTTA+AGG + chr3_3:65886624-65886643 Msa0465480:CDS 40.0%
AGAGTGATGTGATCACAAGT+TGG + chr3_3:65886855-65886874 Msa0465480:intron 40.0%
GATGAAAGAGAAAGGGAAGA+AGG - chr3_3:65886966-65886985 None:intergenic 40.0%
CGTTCTCAGATGAAAGAGAA+AGG - chr3_3:65886974-65886993 None:intergenic 40.0%
ATCGAAACAGAAGATGGAGT+AGG - chr3_3:65887108-65887127 None:intergenic 40.0%
ACAGATGGAAGGAAAACTCA+TGG - chr3_3:65887266-65887285 None:intergenic 40.0%
CCCTAAAAACAACAGATGGA+AGG - chr3_3:65887277-65887296 None:intergenic 40.0%
!!! AGCGGGATTGTGTTGTTTTT+AGG + chr3_3:65887305-65887324 Msa0465480:intron 40.0%
!!! GCGGGATTGTGTTGTTTTTA+GGG + chr3_3:65887306-65887325 Msa0465480:intron 40.0%
CGCTGGATATTACAAGCTTA+TGG - chr3_3:65887370-65887389 None:intergenic 40.0%
! CTATTAACCTTTGAAGGACG+GGG - chr3_3:65887553-65887572 None:intergenic 40.0%
GAGCCAGACAAAAAAGTTTG+GGG - chr3_3:65887627-65887646 None:intergenic 40.0%
GGAGCCAGACAAAAAAGTTT+GGG - chr3_3:65887628-65887647 None:intergenic 40.0%
! TCTACTGCTGCTTATTGTGT+TGG + chr3_3:65888024-65888043 Msa0465480:intron 40.0%
TGCAATTAAAGGTAGGAGGT+AGG - chr3_3:65888165-65888184 None:intergenic 40.0%
ACCGATGAAACCTTTCTTGA+AGG + chr3_3:65888380-65888399 Msa0465480:three_prime_UTR 40.0%
CATGACCATTCCTTCAAGAA+AGG - chr3_3:65888393-65888412 None:intergenic 40.0%
! CATTGTTGCTCTTGTTGTCT+TGG + chr3_3:65888484-65888503 Msa0465480:three_prime_UTR 40.0%
GATGTAAGTCTTCTCGTTTC+AGG + chr3_3:65888560-65888579 Msa0465480:three_prime_UTR 40.0%
CTCGTAAGAGTTTGGTAGAT+AGG - chr3_3:65888689-65888708 None:intergenic 40.0%
CTATCTACCAAACTCTTACG+AGG + chr3_3:65888687-65888706 Msa0465480:three_prime_UTR 40.0%
! TAATGGACCTCGTAAGAGTT+TGG - chr3_3:65888697-65888716 None:intergenic 40.0%
ACGAGAAGCAATAAAAACCG+AGG - chr3_3:65888867-65888886 None:intergenic 40.0%
! GCAAACACATGTAGTCTTGA+TGG - chr3_3:65888893-65888912 None:intergenic 40.0%
CTGCAACGAGAATAACATGA+TGG - chr3_3:65888915-65888934 None:intergenic 40.0%
CATCATGTTATTCTCGTTGC+AGG + chr3_3:65888913-65888932 Msa0465480:three_prime_UTR 40.0%
ATGTAACCAGTGGAGCAAAA+AGG - chr3_3:65888943-65888962 None:intergenic 40.0%
!! TGTGCTGCTACTTAGTTTGT+TGG + chr3_3:65889063-65889082 Msa0465480:three_prime_UTR 40.0%
!! TCAACTTGGTTTGCCCTTAT+TGG + chr3_3:65889092-65889111 Msa0465480:three_prime_UTR 40.0%
!!! TCACTACCTGGTCTAGTTTT+AGG + chr3_3:65889262-65889281 Msa0465480:three_prime_UTR 40.0%
! TTGCTGTTCAAAGTTAGAGG+AGG + chr3_3:65889306-65889325 Msa0465480:three_prime_UTR 40.0%
TGCTGTTCAAAGTTAGAGGA+GGG + chr3_3:65889307-65889326 Msa0465480:three_prime_UTR 40.0%
TCAATCTCTGCCTCCATTTC+CGG + chr3_3:65886547-65886566 Msa0465480:CDS 45.0%
CCTAACAACGATCTGAGAGA+GGG - chr3_3:65886621-65886640 None:intergenic 45.0%
ACCTAACAACGATCTGAGAG+AGG - chr3_3:65886622-65886641 None:intergenic 45.0%
ACAGAAGATGGAGTAGGATG+CGG - chr3_3:65887102-65887121 None:intergenic 45.0%
AAATGATCCCCGTCCTTCAA+AGG + chr3_3:65887543-65887562 Msa0465480:intron 45.0%
!!! GTATTTTGATGGTCGGATGC+CGG + chr3_3:65887598-65887617 Msa0465480:intron 45.0%
! ACAAAAAAGTTTGGGGTGGC+CGG - chr3_3:65887620-65887639 None:intergenic 45.0%
CCAGACAAAAAAGTTTGGGG+TGG - chr3_3:65887624-65887643 None:intergenic 45.0%
!!! CCACCCCAAACTTTTTTGTC+TGG + chr3_3:65887621-65887640 Msa0465480:intron 45.0%
CGGAGCCAGACAAAAAAGTT+TGG - chr3_3:65887629-65887648 None:intergenic 45.0%
CTAATCCAAATGGGCCTGTT+TGG + chr3_3:65887695-65887714 Msa0465480:intron 45.0%
TCAATCCAAACAGGCCCATT+TGG - chr3_3:65887703-65887722 None:intergenic 45.0%
GTATAAGCACCTGTGACACT+GGG + chr3_3:65887748-65887767 Msa0465480:intron 45.0%
ATAAGTTCTCCCAGTGTCAC+AGG - chr3_3:65887760-65887779 None:intergenic 45.0%
!! ACTTTTCTCCATTTGGCTCG+AGG - chr3_3:65888320-65888339 None:intergenic 45.0%
TGGAGAAAAGTAGCCTATGG+TGG + chr3_3:65888329-65888348 Msa0465480:three_prime_UTR 45.0%
GAACATGCTAAAGGTGATGC+TGG + chr3_3:65888433-65888452 Msa0465480:three_prime_UTR 45.0%
! TTGCTCTTGTTGTCTTGGTC+TGG + chr3_3:65888489-65888508 Msa0465480:three_prime_UTR 45.0%
AAATGCTTCTGTAGTCGCCT+CGG + chr3_3:65888847-65888866 Msa0465480:three_prime_UTR 45.0%
! GGTTTTCCTTTTTGCTCCAC+TGG + chr3_3:65888934-65888953 Msa0465480:three_prime_UTR 45.0%
! AGTTTGAGATCAATGGACCC+TGG + chr3_3:65889137-65889156 Msa0465480:three_prime_UTR 45.0%
! GTTTGAGATCAATGGACCCT+GGG + chr3_3:65889138-65889157 Msa0465480:three_prime_UTR 45.0%
! TGAGAAATTGGAGTGCCCTA+TGG + chr3_3:65889217-65889236 Msa0465480:three_prime_UTR 45.0%
!! ATTGGAGTGCCCTATGGTTT+TGG + chr3_3:65889223-65889242 Msa0465480:three_prime_UTR 45.0%
GGAAATGGAGGCAGAGATTG+AGG - chr3_3:65886548-65886567 None:intergenic 50.0%
ATCTGAGAGAGGGAACACAG+AGG - chr3_3:65886611-65886630 None:intergenic 50.0%
CCCTCTCTCAGATCGTTGTT+AGG + chr3_3:65886618-65886637 Msa0465480:CDS 50.0%
! TAAGGTTCGGTCGCTTGCTT+TGG - chr3_3:65887018-65887037 None:intergenic 50.0%
!! GTGGTGGTGGATGTAAGGTT+CGG - chr3_3:65887031-65887050 None:intergenic 50.0%
TGTCACATTCTTTGCAGCGC+TGG - chr3_3:65887387-65887406 None:intergenic 50.0%
GGTATAAGCACCTGTGACAC+TGG + chr3_3:65887747-65887766 Msa0465480:intron 50.0%
TCTGATTCTGCCGTTGCATC+TGG + chr3_3:65888239-65888258 Msa0465480:three_prime_UTR 50.0%
! TTTCTCCATTTGGCTCGAGG+CGG - chr3_3:65888317-65888336 None:intergenic 50.0%
TCCAGGTTGCATACCACCAT+AGG - chr3_3:65888345-65888364 None:intergenic 50.0%
AATCCACTGTCAAAGCCCCA+GGG + chr3_3:65888786-65888805 Msa0465480:three_prime_UTR 50.0%
CAGAGGTAATCGCCGGAAGA+GGG - chr3_3:65886594-65886613 None:intergenic 55.0%
ACAGAGGTAATCGCCGGAAG+AGG - chr3_3:65886595-65886614 None:intergenic 55.0%
GGGAACACAGAGGTAATCGC+CGG - chr3_3:65886601-65886620 None:intergenic 55.0%
TTCGGTCGCTTGCTTTGGTC+GGG - chr3_3:65887013-65887032 None:intergenic 55.0%
GTTCGGTCGCTTGCTTTGGT+CGG - chr3_3:65887014-65887033 None:intergenic 55.0%
!! GTGGTGTGGTGGTGGATGTA+AGG - chr3_3:65887036-65887055 None:intergenic 55.0%
TGGAAAGGAGAGAGTGGTCG+CGG - chr3_3:65887069-65887088 None:intergenic 55.0%
GACGGATGGAAAGGAGAGAG+TGG - chr3_3:65887075-65887094 None:intergenic 55.0%
GAAGATGGAGTAGGATGCGG+CGG - chr3_3:65887099-65887118 None:intergenic 55.0%
GCCTATGGTGGTATGCAACC+TGG + chr3_3:65888341-65888360 Msa0465480:three_prime_UTR 55.0%
GAATCCACTGTCAAAGCCCC+AGG + chr3_3:65888785-65888804 Msa0465480:three_prime_UTR 55.0%
ATCCACTGTCAAAGCCCCAG+GGG + chr3_3:65888787-65888806 Msa0465480:three_prime_UTR 55.0%
AGGGTTCTTCAAAGCCCCTG+GGG - chr3_3:65888804-65888823 None:intergenic 55.0%
CAGGGTTCTTCAAAGCCCCT+GGG - chr3_3:65888805-65888824 None:intergenic 55.0%
CAAAGCTTAGCCTCGTCCCA+GGG - chr3_3:65889157-65889176 None:intergenic 55.0%
ACAAAGCTTAGCCTCGTCCC+AGG - chr3_3:65889158-65889177 None:intergenic 55.0%
!!! GGAGTGCCCTATGGTTTTGG+TGG + chr3_3:65889226-65889245 Msa0465480:three_prime_UTR 55.0%
AAGAGGGAGAGAGAAGCGGC+GGG - chr3_3:65886578-65886597 None:intergenic 60.0%
GAAGAGGGAGAGAGAAGCGG+CGG - chr3_3:65886579-65886598 None:intergenic 60.0%
CCGGAAGAGGGAGAGAGAAG+CGG - chr3_3:65886582-65886601 None:intergenic 60.0%
CCGCTTCTCTCTCCCTCTTC+CGG + chr3_3:65886579-65886598 Msa0465480:CDS 60.0%
CTTCTCCGCCTCGAGCCAAA+TGG + chr3_3:65888309-65888328 Msa0465480:three_prime_UTR 60.0%
GCAGGGTTCTTCAAAGCCCC+TGG - chr3_3:65888806-65888825 None:intergenic 60.0%
GATCAATGGACCCTGGGACG+AGG + chr3_3:65889144-65889163 Msa0465480:three_prime_UTR 60.0%
ATCCACCACCACACCACCGC+CGG + chr3_3:65887039-65887058 Msa0465480:CDS 65.0%
!! TGCGGCGGTGACGGATGGAA+AGG - chr3_3:65887084-65887103 None:intergenic 65.0%
!! TAGGATGCGGCGGTGACGGA+TGG - chr3_3:65887089-65887108 None:intergenic 65.0%
GGAGTAGGATGCGGCGGTGA+CGG - chr3_3:65887093-65887112 None:intergenic 65.0%
! AGCCCCTGGGGCTTTGACAG+TGG - chr3_3:65888792-65888811 None:intergenic 65.0%
AGGGAGAGAGAAGCGGCGGG+CGG - chr3_3:65886575-65886594 None:intergenic 70.0%
AGAGAGTGGTCGCGGCTCGC+CGG - chr3_3:65887061-65887080 None:intergenic 70.0%
! GCGGGCGGCACCGGAAATGG+AGG - chr3_3:65886560-65886579 None:intergenic 75.0%
AGAGAAGCGGCGGGCGGCAC+CGG - chr3_3:65886569-65886588 None:intergenic 75.0%
!! GCGGCGGGCGGCACCGGAAA+TGG - chr3_3:65886563-65886582 None:intergenic 80.0%
! CGCCGGCGGTGGTGTGGTGG+TGG - chr3_3:65887044-65887063 None:intergenic 80.0%
! GCTCGCCGGCGGTGGTGTGG+TGG - chr3_3:65887047-65887066 None:intergenic 80.0%
! GAGTGGTCGCGGCTCGCCGG+CGG - chr3_3:65887058-65887077 None:intergenic 80.0%
! GCGGCTCGCCGGCGGTGGTG+TGG - chr3_3:65887050-65887069 None:intergenic 85.0%
! TGGTCGCGGCTCGCCGGCGG+TGG - chr3_3:65887055-65887074 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 65886509 65889524 65886509 ID=Msa0465480;Name=Msa0465480
chr3_3 mRNA 65886509 65889524 65886509 ID=Msa0465480-mRNA-1;Parent=Msa0465480;Name=Msa0465480-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|1|1|1|0.5|0.66|3|1323|165
chr3_3 exon 65886509 65886645 65886509 ID=Msa0465480-mRNA-1:exon:14677;Parent=Msa0465480-mRNA-1
chr3_3 exon 65886942 65887191 65886942 ID=Msa0465480-mRNA-1:exon:14678;Parent=Msa0465480-mRNA-1
chr3_3 exon 65888091 65889524 65888091 ID=Msa0465480-mRNA-1:exon:14679;Parent=Msa0465480-mRNA-1
chr3_3 CDS 65886509 65886645 65886509 ID=Msa0465480-mRNA-1:cds;Parent=Msa0465480-mRNA-1
chr3_3 CDS 65886942 65887191 65886942 ID=Msa0465480-mRNA-1:cds;Parent=Msa0465480-mRNA-1
chr3_3 CDS 65888091 65888201 65888091 ID=Msa0465480-mRNA-1:cds;Parent=Msa0465480-mRNA-1
chr3_3 three_prime_UTR 65888202 65889524 65888202 ID=Msa0465480-mRNA-1:three_prime_utr;Parent=Msa0465480-mRNA-1
Gene Sequence

>Msa0465480

ATATTTACAAATTACACAACCCTCTTATCTGTTACTCCTCAATCTCTGCCTCCATTTCCGGTGCCGCCCGCCGCTTCTCTCTCCCTCTTCCGGCGATTACCTCTGTGTTCCCTCTCTCAGATCGTTGTTAGGTTAAGAATTCCTATTCAGTTTCATCTCCTTCTTCCCTTTCTCTTTCATCTGAGAACGCGATCTCTATTCTTCTCCCGACCAAAGCAAGCGACCGAACCTTACATCCACCACCACACCACCGCCGGCGAGCCGCGACCACTCTCTCCTTTCCATCCGTCACCGCCGCATCCTACTCCATCTTCTGTTTCGATTCTTCAGTTTAGAGAGCTATCAACATCTCTCTCACAACAAACCCTATTTGTTGTAACTTGTAAGTTCGGAGATGCATTTGATCTAAATTTAGACGATTGGAAGACAATGGAGTGTGCAATGAAAATATCATGTCGCCTACCTCCTACCTTTAATTGCATATACCCAAAGATTTGA
Protein sequence

>Msa0465480

IFTNYTTLLSVTPQSLPPFPVPPAASLSLFRRLPLCSLSQIVVRLRIPIQFHLLLPFLFHLRTRSLFFSRPKQATEPYIHHHTTAGEPRPLSPFHPSPPHPTPSSVSILQFRELSTSLSQQTLFVVTCKFGDAFDLNLDDWKTMECAMKISCRLPPTFNCIYPKI*