AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0946080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0946080 MtrunA17_Chr2g0333051 82.963 135 5 1 1 117 1 135 3.02e-67 199
Msa0946080 MtrunA17_Chr4g0003391 46.565 131 47 4 1 116 1 123 1.17e-23 88.2
Msa0946080 MtrunA17_Chr4g0063791 34.058 138 63 4 5 115 35 171 5.98e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0946080 AT1G52565.1 39.535 129 60 3 1 117 4 126 5.19e-14 63.9
Msa0946080 AT3G15760.1 38.686 137 59 6 1 117 1 132 1.34e-13 63.2

Find 28 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCATCATTTCAAATTCT+TGG 0.191389 6_3:+26403202 Msa0946080:CDS
CAAAATATCATTTCTCTTTG+TGG 0.282402 6_3:+26404137 Msa0946080:three_prime_UTR
TACAAAGCCATTCTATTTGC+AGG 0.298881 6_3:-26403808 None:intergenic
CATCACTAGTTATTTGAGAT+TGG 0.337453 6_3:+26404247 Msa0946080:three_prime_UTR
CTTCCAAGAATTTGAAATGA+TGG 0.348228 6_3:-26403205 None:intergenic
GATAAAATACACTGCTTCAT+AGG 0.352001 6_3:-26403904 None:intergenic
TGATGGGTTAATCTTGTGTT+TGG 0.357672 6_3:-26403188 None:intergenic
TATCATTTCTCTTTGTGGTA+AGG 0.404566 6_3:+26404142 Msa0946080:three_prime_UTR
ATAGAATGGCTTTGTATAAC+GGG 0.410953 6_3:+26403815 Msa0946080:CDS
TCAACAAAGTTGTCATCAAA+TGG 0.417779 6_3:-26404019 None:intergenic
TTGAATGAAACTTGGGAAAG+AGG 0.436103 6_3:+26403877 Msa0946080:three_prime_UTR
ATCATTTCTCTTTGTGGTAA+GGG 0.442617 6_3:+26404143 Msa0946080:three_prime_UTR
TCAAATAACTAGTGATGAGT+TGG 0.448071 6_3:-26404241 None:intergenic
AATAGAATGGCTTTGTATAA+CGG 0.457196 6_3:+26403814 Msa0946080:CDS
TTCCAAGAATTTGAAATGAT+GGG 0.463098 6_3:-26403204 None:intergenic
GATTCTTGTTGAATGAAACT+TGG 0.465140 6_3:+26403869 Msa0946080:exon
ACAAATGACTTCTCTCTCAC+AGG 0.497730 6_3:+26403108 None:intergenic
CTGTTGTGTGGTTGTAGTGT+GGG 0.501190 6_3:+26404183 Msa0946080:three_prime_UTR
TAGATTTGAAGAAAGTGTGA+AGG 0.513088 6_3:+26403684 Msa0946080:CDS
TGGAATTCCTGCAAATAGAA+TGG 0.533064 6_3:+26403801 Msa0946080:CDS
ATTCTTGTTGAATGAAACTT+GGG 0.544886 6_3:+26403870 Msa0946080:exon
TTTCTCAGAGGAAAAGAAGA+GGG 0.546313 6_3:+26403624 Msa0946080:intron
ACTGTTGTGTGGTTGTAGTG+TGG 0.565398 6_3:+26404182 Msa0946080:three_prime_UTR
CAAATGACTTCTCTCTCACA+GGG 0.592780 6_3:+26403109 None:intergenic
GATAATGTCAAATCTAGTTG+TGG 0.616027 6_3:-26404216 None:intergenic
AGTGAAACAACGAAAACTCG+TGG 0.634965 6_3:+26403781 Msa0946080:CDS
TGTTGTGTGGTTGTAGTGTG+GGG 0.661609 6_3:+26404184 Msa0946080:three_prime_UTR
ACACATACACAACTGTTGTG+TGG 0.669472 6_3:+26404171 Msa0946080:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCGATATTGATATTAATTA+AGG - chr6_3:26403508-26403527 None:intergenic 15.0%
!! ATCGATATTGATATTAATTA+AGG - chr6_3:26403508-26403527 None:intergenic 15.0%
!! TCTACATACAAAATAAAGTA+AGG - chr6_3:26403312-26403331 None:intergenic 20.0%
!!! TTACTTTATTTTGTATGTAG+AGG + chr6_3:26403311-26403330 Msa0946080:intron 20.0%
!!! ATATCGATATTGTTATTGTT+AGG - chr6_3:26403428-26403447 None:intergenic 20.0%
!! AATATCAATATCGATATTGT+CGG + chr6_3:26403513-26403532 Msa0946080:intron 20.0%
!! AAAATACACACAATATAGTA+AGG - chr6_3:26403604-26403623 None:intergenic 20.0%
!! TCTACATACAAAATAAAGTA+AGG - chr6_3:26403312-26403331 None:intergenic 20.0%
!!! TTACTTTATTTTGTATGTAG+AGG + chr6_3:26403311-26403330 Msa0946080:intron 20.0%
!!! ATATCGATATTGTTATTGTT+AGG - chr6_3:26403428-26403447 None:intergenic 20.0%
!! AATATCAATATCGATATTGT+CGG + chr6_3:26403513-26403532 Msa0946080:intron 20.0%
!! AAAATACACACAATATAGTA+AGG - chr6_3:26403604-26403623 None:intergenic 20.0%
!! TTCCAAGAATTTGAAATGAT+GGG - chr6_3:26403207-26403226 None:intergenic 25.0%
!!! TTTTTTATGACCTAACTCAA+CGG - chr6_3:26403454-26403473 None:intergenic 25.0%
!!! AATAGAATGGCTTTGTATAA+CGG + chr6_3:26403814-26403833 Msa0946080:CDS 25.0%
! ATTCTTGTTGAATGAAACTT+GGG + chr6_3:26403870-26403889 Msa0946080:exon 25.0%
! TCAAAGAAAAACAAAAACAG+AGG - chr6_3:26403947-26403966 None:intergenic 25.0%
!!! TGTTTTTGTTTTTCTTTGAG+TGG + chr6_3:26403948-26403967 Msa0946080:three_prime_UTR 25.0%
!!! TGAATTCTAGTTTAGTTTTG+TGG + chr6_3:26403974-26403993 Msa0946080:three_prime_UTR 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr6_3:26404048-26404067 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr6_3:26404047-26404066 Msa0946080:three_prime_UTR 25.0%
! CAAAATATCATTTCTCTTTG+TGG + chr6_3:26404137-26404156 Msa0946080:three_prime_UTR 25.0%
!! ATATAGTGAGTGATATTTTG+TGG + chr6_3:26404305-26404324 Msa0946080:three_prime_UTR 25.0%
!! TTCCAAGAATTTGAAATGAT+GGG - chr6_3:26403207-26403226 None:intergenic 25.0%
!!! TTTTTTATGACCTAACTCAA+CGG - chr6_3:26403454-26403473 None:intergenic 25.0%
!!! AATAGAATGGCTTTGTATAA+CGG + chr6_3:26403814-26403833 Msa0946080:CDS 25.0%
! ATTCTTGTTGAATGAAACTT+GGG + chr6_3:26403870-26403889 Msa0946080:exon 25.0%
! TCAAAGAAAAACAAAAACAG+AGG - chr6_3:26403947-26403966 None:intergenic 25.0%
!!! TGTTTTTGTTTTTCTTTGAG+TGG + chr6_3:26403948-26403967 Msa0946080:three_prime_UTR 25.0%
!!! TGAATTCTAGTTTAGTTTTG+TGG + chr6_3:26403974-26403993 Msa0946080:three_prime_UTR 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr6_3:26404048-26404067 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr6_3:26404047-26404066 Msa0946080:three_prime_UTR 25.0%
! CAAAATATCATTTCTCTTTG+TGG + chr6_3:26404137-26404156 Msa0946080:three_prime_UTR 25.0%
!! ATATAGTGAGTGATATTTTG+TGG + chr6_3:26404305-26404324 Msa0946080:three_prime_UTR 25.0%
AACCCATCATTTCAAATTCT+TGG + chr6_3:26403202-26403221 Msa0946080:CDS 30.0%
! CTTCCAAGAATTTGAAATGA+TGG - chr6_3:26403208-26403227 None:intergenic 30.0%
ATAGTAAGGACTAAGAACTA+AGG - chr6_3:26403590-26403609 None:intergenic 30.0%
!!! TGTGTGTATTTTTTTCTCAG+AGG + chr6_3:26403612-26403631 Msa0946080:intron 30.0%
! TAGATTTGAAGAAAGTGTGA+AGG + chr6_3:26403684-26403703 Msa0946080:CDS 30.0%
TAGCAAAAAAAGAGAAACAG+AGG + chr6_3:26403728-26403747 Msa0946080:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG + chr6_3:26403815-26403834 Msa0946080:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG + chr6_3:26403825-26403844 Msa0946080:CDS 30.0%
GATTCTTGTTGAATGAAACT+TGG + chr6_3:26403869-26403888 Msa0946080:exon 30.0%
GATAAAATACACTGCTTCAT+AGG - chr6_3:26403907-26403926 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr6_3:26404022-26404041 None:intergenic 30.0%
!!! AACCACTTTTTGACAACTTT+TGG - chr6_3:26404115-26404134 None:intergenic 30.0%
TATCATTTCTCTTTGTGGTA+AGG + chr6_3:26404142-26404161 Msa0946080:three_prime_UTR 30.0%
ATCATTTCTCTTTGTGGTAA+GGG + chr6_3:26404143-26404162 Msa0946080:three_prime_UTR 30.0%
GATAATGTCAAATCTAGTTG+TGG - chr6_3:26404219-26404238 None:intergenic 30.0%
TCAAATAACTAGTGATGAGT+TGG - chr6_3:26404244-26404263 None:intergenic 30.0%
! CATCACTAGTTATTTGAGAT+TGG + chr6_3:26404247-26404266 Msa0946080:three_prime_UTR 30.0%
AACCCATCATTTCAAATTCT+TGG + chr6_3:26403202-26403221 Msa0946080:CDS 30.0%
! CTTCCAAGAATTTGAAATGA+TGG - chr6_3:26403208-26403227 None:intergenic 30.0%
ATAGTAAGGACTAAGAACTA+AGG - chr6_3:26403590-26403609 None:intergenic 30.0%
!!! TGTGTGTATTTTTTTCTCAG+AGG + chr6_3:26403612-26403631 Msa0946080:intron 30.0%
! TAGATTTGAAGAAAGTGTGA+AGG + chr6_3:26403684-26403703 Msa0946080:CDS 30.0%
TAGCAAAAAAAGAGAAACAG+AGG + chr6_3:26403728-26403747 Msa0946080:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG + chr6_3:26403815-26403834 Msa0946080:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG + chr6_3:26403825-26403844 Msa0946080:CDS 30.0%
GATTCTTGTTGAATGAAACT+TGG + chr6_3:26403869-26403888 Msa0946080:exon 30.0%
GATAAAATACACTGCTTCAT+AGG - chr6_3:26403907-26403926 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr6_3:26404022-26404041 None:intergenic 30.0%
!!! AACCACTTTTTGACAACTTT+TGG - chr6_3:26404115-26404134 None:intergenic 30.0%
TATCATTTCTCTTTGTGGTA+AGG + chr6_3:26404142-26404161 Msa0946080:three_prime_UTR 30.0%
ATCATTTCTCTTTGTGGTAA+GGG + chr6_3:26404143-26404162 Msa0946080:three_prime_UTR 30.0%
GATAATGTCAAATCTAGTTG+TGG - chr6_3:26404219-26404238 None:intergenic 30.0%
TCAAATAACTAGTGATGAGT+TGG - chr6_3:26404244-26404263 None:intergenic 30.0%
! CATCACTAGTTATTTGAGAT+TGG + chr6_3:26404247-26404266 Msa0946080:three_prime_UTR 30.0%
GGAAAAAGCTAGATAAAGCA+TGG - chr6_3:26403170-26403189 None:intergenic 35.0%
!! TGATGGGTTAATCTTGTGTT+TGG - chr6_3:26403191-26403210 None:intergenic 35.0%
AGCGCAAAATTTGTGTGTTA+GGG - chr6_3:26403246-26403265 None:intergenic 35.0%
! TTGCGCTCTTGTTTATATTC+AGG + chr6_3:26403259-26403278 Msa0946080:intron 35.0%
AGGTTAATCAAAAGAAGAGC+TGG - chr6_3:26403292-26403311 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr6_3:26403484-26403503 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr6_3:26403484-26403503 None:intergenic 35.0%
! TTTTCTCAGAGGAAAAGAAG+AGG + chr6_3:26403623-26403642 Msa0946080:intron 35.0%
TTTCTCAGAGGAAAAGAAGA+GGG + chr6_3:26403624-26403643 Msa0946080:intron 35.0%
TGGAATTCCTGCAAATAGAA+TGG + chr6_3:26403801-26403820 Msa0946080:CDS 35.0%
TACAAAGCCATTCTATTTGC+AGG - chr6_3:26403811-26403830 None:intergenic 35.0%
!! CTTTGTATAACGGGATTTTG+AGG + chr6_3:26403824-26403843 Msa0946080:CDS 35.0%
TTGAATGAAACTTGGGAAAG+AGG + chr6_3:26403877-26403896 Msa0946080:three_prime_UTR 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG + chr6_3:26404110-26404129 Msa0946080:three_prime_UTR 35.0%
! GAGTGATATTTTGTGGTAAG+TGG + chr6_3:26404312-26404331 Msa0946080:three_prime_UTR 35.0%
GGAAAAAGCTAGATAAAGCA+TGG - chr6_3:26403170-26403189 None:intergenic 35.0%
!! TGATGGGTTAATCTTGTGTT+TGG - chr6_3:26403191-26403210 None:intergenic 35.0%
AGCGCAAAATTTGTGTGTTA+GGG - chr6_3:26403246-26403265 None:intergenic 35.0%
! TTGCGCTCTTGTTTATATTC+AGG + chr6_3:26403259-26403278 Msa0946080:intron 35.0%
AGGTTAATCAAAAGAAGAGC+TGG - chr6_3:26403292-26403311 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr6_3:26403484-26403503 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr6_3:26403484-26403503 None:intergenic 35.0%
! TTTTCTCAGAGGAAAAGAAG+AGG + chr6_3:26403623-26403642 Msa0946080:intron 35.0%
TTTCTCAGAGGAAAAGAAGA+GGG + chr6_3:26403624-26403643 Msa0946080:intron 35.0%
TGGAATTCCTGCAAATAGAA+TGG + chr6_3:26403801-26403820 Msa0946080:CDS 35.0%
TACAAAGCCATTCTATTTGC+AGG - chr6_3:26403811-26403830 None:intergenic 35.0%
!! CTTTGTATAACGGGATTTTG+AGG + chr6_3:26403824-26403843 Msa0946080:CDS 35.0%
TTGAATGAAACTTGGGAAAG+AGG + chr6_3:26403877-26403896 Msa0946080:three_prime_UTR 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG + chr6_3:26404110-26404129 Msa0946080:three_prime_UTR 35.0%
! GAGTGATATTTTGTGGTAAG+TGG + chr6_3:26404312-26404331 Msa0946080:three_prime_UTR 35.0%
GAGCGCAAAATTTGTGTGTT+AGG - chr6_3:26403247-26403266 None:intergenic 40.0%
TACTGAAACTCACACGCATA+AGG + chr6_3:26403345-26403364 Msa0946080:intron 40.0%
TCGATATTGTCCGTTGAGTT+AGG + chr6_3:26403441-26403460 Msa0946080:intron 40.0%
! TCGATATTGTCGGTTAAGCT+AGG + chr6_3:26403523-26403542 Msa0946080:intron 40.0%
AGTGAAACAACGAAAACTCG+TGG + chr6_3:26403781-26403800 Msa0946080:CDS 40.0%
ACACATACACAACTGTTGTG+TGG + chr6_3:26404171-26404190 Msa0946080:three_prime_UTR 40.0%
GAGCGCAAAATTTGTGTGTT+AGG - chr6_3:26403247-26403266 None:intergenic 40.0%
TACTGAAACTCACACGCATA+AGG + chr6_3:26403345-26403364 Msa0946080:intron 40.0%
TCGATATTGTCCGTTGAGTT+AGG + chr6_3:26403441-26403460 Msa0946080:intron 40.0%
! TCGATATTGTCGGTTAAGCT+AGG + chr6_3:26403523-26403542 Msa0946080:intron 40.0%
AGTGAAACAACGAAAACTCG+TGG + chr6_3:26403781-26403800 Msa0946080:CDS 40.0%
ACACATACACAACTGTTGTG+TGG + chr6_3:26404171-26404190 Msa0946080:three_prime_UTR 40.0%
ACACACAACTGTGTGGTACA+GGG + chr6_3:26403377-26403396 Msa0946080:intron 45.0%
!! TTTTGAGGGATCGTGTGCAT+AGG + chr6_3:26403839-26403858 Msa0946080:CDS 45.0%
ACTGTTGTGTGGTTGTAGTG+TGG + chr6_3:26404182-26404201 Msa0946080:three_prime_UTR 45.0%
CTGTTGTGTGGTTGTAGTGT+GGG + chr6_3:26404183-26404202 Msa0946080:three_prime_UTR 45.0%
TGTTGTGTGGTTGTAGTGTG+GGG + chr6_3:26404184-26404203 Msa0946080:three_prime_UTR 45.0%
ACACACAACTGTGTGGTACA+GGG + chr6_3:26403377-26403396 Msa0946080:intron 45.0%
!! TTTTGAGGGATCGTGTGCAT+AGG + chr6_3:26403839-26403858 Msa0946080:CDS 45.0%
ACTGTTGTGTGGTTGTAGTG+TGG + chr6_3:26404182-26404201 Msa0946080:three_prime_UTR 45.0%
CTGTTGTGTGGTTGTAGTGT+GGG + chr6_3:26404183-26404202 Msa0946080:three_prime_UTR 45.0%
TGTTGTGTGGTTGTAGTGTG+GGG + chr6_3:26404184-26404203 Msa0946080:three_prime_UTR 45.0%
CGAACTCACACACAACTGTG+TGG + chr6_3:26403370-26403389 Msa0946080:intron 50.0%
CACACACAACTGTGTGGTAC+AGG + chr6_3:26403376-26403395 Msa0946080:intron 50.0%
TGTGGTACAGGGTTCGAACT+CGG + chr6_3:26403388-26403407 Msa0946080:intron 50.0%
CGAACTCACACACAACTGTG+TGG + chr6_3:26403370-26403389 Msa0946080:intron 50.0%
CACACACAACTGTGTGGTAC+AGG + chr6_3:26403376-26403395 Msa0946080:intron 50.0%
TGTGGTACAGGGTTCGAACT+CGG + chr6_3:26403388-26403407 Msa0946080:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 26403112 26404344 26403112 ID=Msa0946080;Name=Msa0946080
chr6_3 mRNA 26403112 26404344 26403112 ID=Msa0946080-mRNA-1;Parent=Msa0946080;Name=Msa0946080-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0.5|1|0|0.5|2|474|117
chr6_3 exon 26403112 26403226 26403112 ID=Msa0946080-mRNA-1:exon:7291;Parent=Msa0946080-mRNA-1
chr6_3 exon 26403632 26404344 26403632 ID=Msa0946080-mRNA-1:exon:7292;Parent=Msa0946080-mRNA-1
chr6_3 CDS 26403112 26403226 26403112 ID=Msa0946080-mRNA-1:cds;Parent=Msa0946080-mRNA-1
chr6_3 CDS 26403632 26403870 26403632 ID=Msa0946080-mRNA-1:cds;Parent=Msa0946080-mRNA-1
chr6_3 three_prime_UTR 26403871 26404344 26403871 ID=Msa0946080-mRNA-1:three_prime_utr;Parent=Msa0946080-mRNA-1
Gene Sequence

>Msa0946080

ATGACTTCTCTCTCACAGGGTCTTGTTTTCACTACAGCTATGCTTGTATCAAGCACCATGCTTTATCTAGCTTTTTCCAAACACAAGATTAACCCATCATTTCAAATTCTTGGAAAGGAAAAGAAGAGGGAGAGAAAGATGAATACAAAGAAAAATAAAAAGAAAGTTAGATTTGAAGAAAGTGTGAAGGAAGTAAGAGAAAAGAGTGAGATAGCAAAAAAAGAGAAACAGAGGAAACGAAACAGAGTACATAGCAACTGCAGAAGTGAAACAACGAAAACTCGTGGAATTCCTGCAAATAGAATGGCTTTGTATAACGGGATTTTGAGGGATCGTGTGCATAGGATTGAGTGA

Protein sequence

>Msa0946080

MTSLSQGLVFTTAMLVSSTMLYLAFSKHKINPSFQILGKEKKRERKMNTKKNKKKVRFEESVKEVREKSEIAKKEKQRKRNRVHSNCRSETTKTRGIPANRMALYNGILRDRVHRIE*