AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0727720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0727720 MtrunA17_Chr5g0420191 94.932 592 24 4 1 591 1 587 0.0 1066
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0727720 AT1G69060.1 60.479 167 62 2 420 585 462 625 1.03e-53 194

Find 138 sgRNAs with CRISPR-Local

Find 380 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATCCAGCCATTAAATT+TGG 0.111745 5_1:+31033771 None:intergenic
TACAGCCCATCTTCAAAATT+TGG 0.160934 5_1:+31033704 None:intergenic
CCCTTTCGCCTGACCAGTTT+TGG 0.189270 5_1:+31038406 None:intergenic
AGAAATCGTTATGGTTTATA+TGG 0.200313 5_1:-31037972 Msa0727720:CDS
TTGAAATTGGAATTGAGATT+TGG 0.225692 5_1:+31039318 None:intergenic
ATTCTTCCATGTCGTGTATT+TGG 0.231268 5_1:-31033679 Msa0727720:three_prime_UTR
AATGAGCATGTCAGTTCTTC+TGG 0.236888 5_1:-31037849 Msa0727720:CDS
AAATTTAATGGCTGGATAAT+AGG 0.237961 5_1:-31033769 Msa0727720:three_prime_UTR
ATTATCCAGCCATTAAATTT+GGG 0.259959 5_1:+31033772 None:intergenic
TTATATAGTCGGACTGGATA+TGG 0.265009 5_1:-31033891 Msa0727720:three_prime_UTR
AGAGGATACCGCAATGAAAA+AGG 0.281900 5_1:-31037883 Msa0727720:CDS
TGAAATTGGAATTGAGATTT+GGG 0.294981 5_1:+31039319 None:intergenic
TGATGACGAGTCTACATTTA+AGG 0.306104 5_1:-31037589 Msa0727720:CDS
CTTAAGCATAGCATGTTTAT+AGG 0.316113 5_1:+31034033 None:intergenic
CGATTTCTTTCTTTAGCGTT+TGG 0.328724 5_1:+31037987 None:intergenic
CAGCCCCGAACATGTTCTTC+GGG 0.342474 5_1:-31038440 Msa0727720:CDS
GGCTGAGAATAGCCGATTGC+TGG 0.345114 5_1:-31035389 Msa0727720:CDS
ATAACATAGAAGCGGAAAAT+AGG 0.354976 5_1:-31037806 Msa0727720:CDS
GACTGTGATGTCTATGAAAA+TGG 0.355128 5_1:-31038077 Msa0727720:CDS
AGAAAGGACAACTAAGAAAA+AGG 0.357996 5_1:-31037691 Msa0727720:CDS
GTCATCACCCAGTTCATTCC+TGG 0.362711 5_1:+31037605 None:intergenic
CTTCTGCTTCTTCGCTTTCA+AGG 0.363881 5_1:-31039263 Msa0727720:intron
GTGTCTCTCTCACTTCTTCA+AGG 0.364573 5_1:+31035341 None:intergenic
AATTTCTGTAACGGTGTTGC+TGG 0.370750 5_1:-31037513 Msa0727720:CDS
CTTCCCCGAAGAACATGTTC+GGG 0.372830 5_1:+31038436 None:intergenic
AAAGAAGCAGCTCAATCATT+TGG 0.376881 5_1:-31034990 Msa0727720:CDS
TAAACACAACTTGCCTCCTT+TGG 0.390211 5_1:+31034893 None:intergenic
ACAGAGACAATTGCAAATTC+AGG 0.403793 5_1:-31037139 Msa0727720:intron
TTCAAAGTCCAGGAATGAAC+TGG 0.404582 5_1:-31037613 Msa0727720:CDS
ACCTCGCTGCTTCGTGGCTT+AGG 0.405340 5_1:-31034947 Msa0727720:CDS
AATTTCTGTAACGATGGTAT+TGG 0.406873 5_1:-31037639 Msa0727720:CDS
GGGAAGAATGCATCCAAAAC+TGG 0.409192 5_1:-31038419 Msa0727720:CDS
GGATGCCATTTCCTCTTCCA+TGG 0.411553 5_1:+31037164 None:intergenic
AGATGAAGTGCGAGAGAAAG+AGG 0.411726 5_1:+31039291 None:intergenic
TCAGCCCCGAACATGTTCTT+CGG 0.412628 5_1:-31038441 Msa0727720:CDS
GTCGATGTTCTATCTTTACT+TGG 0.415664 5_1:+31038308 None:intergenic
AATTTGCAATTGTCTCTGTC+TGG 0.419574 5_1:+31037143 None:intergenic
ATTTCTGAAGAGAATTCTAA+TGG 0.431168 5_1:-31037327 Msa0727720:CDS
TTCTTTCAATGTCCAGCAAT+CGG 0.431241 5_1:+31035377 None:intergenic
AAAAGCCATGGAAGAGGAAA+TGG 0.433668 5_1:-31037169 Msa0727720:CDS
CATGGGAAAGGTCCTTTATA+TGG 0.433963 5_1:-31037774 Msa0727720:CDS
TTGTCATCTTTACTCCTCAA+TGG 0.439565 5_1:+31037351 None:intergenic
TCTTCCCCGAAGAACATGTT+CGG 0.440558 5_1:+31038435 None:intergenic
CATGTAAAGGTTACAAACTT+AGG 0.445286 5_1:-31033732 Msa0727720:three_prime_UTR
AATTATATTATATATGATGA+TGG 0.449700 5_1:+31039343 None:intergenic
CCAAAACTGGTCAGGCGAAA+GGG 0.450590 5_1:-31038406 Msa0727720:CDS
GAAATCGTTATGGTTTATAT+GGG 0.451015 5_1:-31037971 Msa0727720:CDS
CATGTCATGTGCACCTTGGT+TGG 0.453848 5_1:+31037437 None:intergenic
GATATGCCTTTCATCAAAAG+AGG 0.454831 5_1:+31037302 None:intergenic
TATGATGATGTAGAAATCAT+AGG 0.455972 5_1:-31038341 Msa0727720:CDS
ATATGGTCCTAGTTGTAGTA+AGG 0.458017 5_1:-31037757 Msa0727720:CDS
AAGAACTAGGGGCTAGCAAA+AGG 0.458708 5_1:-31033633 Msa0727720:three_prime_UTR
CTACAACTAGGACCATATAA+AGG 0.459702 5_1:+31037762 None:intergenic
TGTTGATGGTGTTGGAGAGT+TGG 0.461044 5_1:-31038177 Msa0727720:CDS
GCGATGCCTCTTCAAACAAA+AGG 0.465308 5_1:-31038151 Msa0727720:CDS
GCTAAAGAAAGAAATCGTTA+TGG 0.468168 5_1:-31037981 Msa0727720:CDS
TCTATCTTTACTTGGCCCAC+TGG 0.468887 5_1:+31038316 None:intergenic
GGAATTGATGTTGATGGTGT+TGG 0.481618 5_1:-31038185 Msa0727720:CDS
GATATGACCTCGCTGCTTCG+TGG 0.484325 5_1:-31034953 Msa0727720:CDS
GATTTCCACGCGGAACTTGA+GGG 0.489022 5_1:+31038119 None:intergenic
TCAAGTTTGAAGCAAGGACT+AGG 0.490468 5_1:-31037486 Msa0727720:CDS
GAATGCATCCAAAACTGGTC+AGG 0.491520 5_1:-31038414 Msa0727720:CDS
TCACTGGTGAGGATGATATT+CGG 0.494559 5_1:-31037719 Msa0727720:CDS
AGATTTCCACGCGGAACTTG+AGG 0.495937 5_1:+31038118 None:intergenic
TTTGATGAAAGGCATATCAA+TGG 0.497644 5_1:-31037297 Msa0727720:CDS
TGTCCGTCATGTGCATTCAA+TGG 0.499716 5_1:+31037393 None:intergenic
TCGCGAGCAGGTTGAGGCTG+AGG 0.502540 5_1:-31033978 Msa0727720:CDS
AACTGTAATTTCTGTAACGA+TGG 0.503873 5_1:-31037645 Msa0727720:CDS
GAGAATCAATCTTCCTTCAC+TGG 0.508407 5_1:-31037735 Msa0727720:CDS
CAGGTATAGATGCTTGGAAA+AGG 0.509013 5_1:-31038482 Msa0727720:exon
CAGTCTGGAATTGATGTTGA+TGG 0.512375 5_1:-31038191 Msa0727720:CDS
TGACATTCGCGAGCAGGTTG+AGG 0.523174 5_1:-31033984 Msa0727720:CDS
CGTGGCTTAGGAGTAAATGT+GGG 0.525737 5_1:-31034935 Msa0727720:CDS
TCCAAAACTGGTCAGGCGAA+AGG 0.525772 5_1:-31038407 Msa0727720:CDS
ATGATATTCGGAATGGAGAA+AGG 0.529183 5_1:-31037707 Msa0727720:CDS
GATGAAGTGCGAGAGAAAGA+GGG 0.534206 5_1:+31039292 None:intergenic
GATAAATTATATAGTCGGAC+TGG 0.534486 5_1:-31033897 Msa0727720:three_prime_UTR
GTGATGCAGGTATAGATGCT+TGG 0.534613 5_1:-31038488 Msa0727720:intron
AGTGATGATGTGGATCAGTC+TGG 0.534934 5_1:-31038206 Msa0727720:CDS
TAAAGAACAAGAACATGTAA+AGG 0.536962 5_1:-31033745 Msa0727720:three_prime_UTR
AGCAAAAGGGATTGAAACAA+AGG 0.537014 5_1:-31033619 Msa0727720:three_prime_UTR
GATGATGATGACGACGATGA+TGG 0.539323 5_1:-31038230 Msa0727720:CDS
CAACTTGCCTCCTTTGGCGA+TGG 0.541317 5_1:+31034899 None:intergenic
TACTGTCATATGAAAGAACT+AGG 0.543049 5_1:-31033646 Msa0727720:three_prime_UTR
GTATTGGTTCTTCAAAGTCC+AGG 0.548601 5_1:-31037623 Msa0727720:CDS
ATTATATTATATATGATGAT+GGG 0.549783 5_1:+31039344 None:intergenic
AGAACTAGGGGCTAGCAAAA+GGG 0.551554 5_1:-31033632 Msa0727720:three_prime_UTR
CGCTAGATAAATTATATAGT+CGG 0.552534 5_1:-31033902 Msa0727720:exon
CAAGCTCATCTCACGCATGA+AGG 0.554012 5_1:-31033948 Msa0727720:CDS
GAAAATAGGTCTGAAAAGCA+TGG 0.554736 5_1:-31037792 Msa0727720:CDS
AAAACGTGAACAGTGGGAAG+AGG 0.555343 5_1:-31037901 Msa0727720:CDS
TATCCAGCCATTAAATTTGG+GGG 0.556909 5_1:+31033774 None:intergenic
TCGTGGCTTAGGAGTAAATG+TGG 0.557564 5_1:-31034936 Msa0727720:CDS
TTTAAGGATGATATTCAGCA+TGG 0.562013 5_1:-31037573 Msa0727720:CDS
CAAAACTGGTCAGGCGAAAG+GGG 0.562765 5_1:-31038405 Msa0727720:CDS
GCAAAAGGGATTGAAACAAA+GGG 0.567756 5_1:-31033618 Msa0727720:three_prime_UTR
AGTGAGAGAGACACTAAAGA+AGG 0.569017 5_1:-31035332 Msa0727720:intron
AATATCATCCTCACCAGTGA+AGG 0.573976 5_1:+31037722 None:intergenic
GGTTCTTCAAGTTTGAAGCA+AGG 0.574153 5_1:-31037492 Msa0727720:CDS
GGATCTGTGATATACTAACT+TGG 0.575711 5_1:-31033597 None:intergenic
GAAAACTCTAATTTCTGTAA+CGG 0.577066 5_1:-31037522 Msa0727720:CDS
TTATCCAGCCATTAAATTTG+GGG 0.582697 5_1:+31033773 None:intergenic
CTTGCATGTCATGTGCACCT+TGG 0.589414 5_1:+31037433 None:intergenic
GAACACGAAAGATGAACTGC+GGG 0.589520 5_1:-31035020 Msa0727720:CDS
GGTGAGGATGATATTCGGAA+TGG 0.591451 5_1:-31037714 Msa0727720:CDS
TGATTCTCCTTACTACAACT+AGG 0.592848 5_1:+31037750 None:intergenic
AAAATAGGTCTGAAAAGCAT+GGG 0.595327 5_1:-31037791 Msa0727720:CDS
TCAATCTTCCTTCACTGGTG+AGG 0.595363 5_1:-31037730 Msa0727720:CDS
AGGTCTGAAAAGCATGGGAA+AGG 0.602874 5_1:-31037786 Msa0727720:CDS
GATTGCTGGACATTGAAAGA+AGG 0.617880 5_1:-31035375 Msa0727720:CDS
ACTGTCATATGAAAGAACTA+GGG 0.619402 5_1:-31033645 Msa0727720:three_prime_UTR
TCTTAGACCATCGCCAAAGG+AGG 0.621225 5_1:-31034906 Msa0727720:CDS
CGATGATGGAAGTGATGATG+TGG 0.622416 5_1:-31038216 Msa0727720:CDS
GTGGCTTAGGAGTAAATGTG+GGG 0.622737 5_1:-31034934 Msa0727720:CDS
CTGTCATATGAAAGAACTAG+GGG 0.623929 5_1:-31033644 Msa0727720:three_prime_UTR
GGTCATAAAACGTGAACAGT+GGG 0.631375 5_1:-31037907 Msa0727720:CDS
AAGTCTTAGACCATCGCCAA+AGG 0.637737 5_1:-31034909 Msa0727720:CDS
TAATTACAATAACATAGAAG+CGG 0.637745 5_1:-31037814 Msa0727720:CDS
TCAAAGTCCAGGAATGAACT+GGG 0.638802 5_1:-31037612 Msa0727720:CDS
AGGTCATAAAACGTGAACAG+TGG 0.641789 5_1:-31037908 Msa0727720:CDS
GTACTACCAAATACACGACA+TGG 0.642905 5_1:+31033673 None:intergenic
TCTCCATTGAATGCACATGA+CGG 0.644527 5_1:-31037396 Msa0727720:CDS
AAATTTATGAGCTCCAACCA+AGG 0.645106 5_1:-31037450 Msa0727720:CDS
AAGCTTCTGCTTCTAACGTG+CGG 0.646962 5_1:-31038025 Msa0727720:CDS
TGACATGCTCATTAAACACA+CGG 0.647259 5_1:+31037859 None:intergenic
ACAATGATGAATCTCCATTG+AGG 0.653343 5_1:-31037365 Msa0727720:CDS
TTCCCCGAAGAACATGTTCG+GGG 0.662747 5_1:+31038437 None:intergenic
GTAGAAATCATAGGATCCAG+TGG 0.663165 5_1:-31038332 Msa0727720:CDS
TAGAAATCATAGGATCCAGT+GGG 0.671951 5_1:-31038331 Msa0727720:CDS
AAAATGGTCCTGTTTCGAAG+AGG 0.678343 5_1:-31038061 Msa0727720:CDS
TAGCGATTGTTCTGATTGTG+AGG 0.680578 5_1:-31037928 Msa0727720:CDS
AGCCCCGAACATGTTCTTCG+GGG 0.683401 5_1:-31038439 Msa0727720:CDS
TGAACACGAAAGATGAACTG+CGG 0.691989 5_1:-31035021 Msa0727720:CDS
TCCTAAGCCACGAAGCAGCG+AGG 0.703909 5_1:+31034946 None:intergenic
TCAATGCTGATAACACTCTG+AGG 0.705556 5_1:+31038284 None:intergenic
CTATGCCCTCAAGTTCCGCG+TGG 0.708335 5_1:-31038124 Msa0727720:CDS
TGGCTTAGGAGTAAATGTGG+GGG 0.719417 5_1:-31034933 Msa0727720:CDS
TACGTATACAGATTTCCACG+CGG 0.870448 5_1:+31038109 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATTATATATGATGAT+GGG + chr5_1:31033736-31033755 None:intergenic 10.0%
!! AATTATATTATATATGATGA+TGG + chr5_1:31033737-31033756 None:intergenic 10.0%
!!! TTGTTTTTAACTTTTAAATT+TGG - chr5_1:31034301-31034320 Msa0727720:intron 10.0%
!! ATTTAAATTGAAATTTGAAA+AGG - chr5_1:31034420-31034439 Msa0727720:intron 10.0%
!! TATCATTTATTAAAATTACT+AGG + chr5_1:31037311-31037330 None:intergenic 10.0%
!!! AGTTAATTTTGTTATTGATT+AGG - chr5_1:31033905-31033924 Msa0727720:exon 15.0%
!!! GTTAATTTTGTTATTGATTA+GGG - chr5_1:31033906-31033925 Msa0727720:exon 15.0%
!!! ATACATAAATTGATTTTGAT+CGG - chr5_1:31034052-31034071 Msa0727720:intron 15.0%
!! ACATATATACCATTATATAA+AGG + chr5_1:31034528-31034547 None:intergenic 15.0%
!!! TTTTTTCTTTGTAATTGTTA+TGG - chr5_1:31037437-31037456 Msa0727720:CDS 15.0%
!!! TATTCTCCTTTATTTAAATT+TGG - chr5_1:31037852-31037871 Msa0727720:CDS 15.0%
!! AATATATTTGAATGTAAATG+TGG + chr5_1:31038838-31038857 None:intergenic 15.0%
!! AAAACAATAGCAATGATTAA+CGG + chr5_1:31033882-31033901 None:intergenic 20.0%
!!! ACCATATATAGTAGAATTTT+TGG - chr5_1:31034136-31034155 Msa0727720:intron 20.0%
!!! CCATATATAGTAGAATTTTT+GGG - chr5_1:31034137-31034156 Msa0727720:intron 20.0%
!! ATTGTTATGAGTAAATTTGT+TGG - chr5_1:31034337-31034356 Msa0727720:intron 20.0%
!! TTGGAAAAAAAATTCAACTA+CGG - chr5_1:31034356-31034375 Msa0727720:intron 20.0%
!!! ATTTTCAAACTTCCTAATTT+AGG - chr5_1:31034386-31034405 Msa0727720:intron 20.0%
!!! TCTAAATTTTTGCCTAAATT+AGG + chr5_1:31034401-31034420 None:intergenic 20.0%
!! ATTGAAATTTGAAAAGGAAT+TGG - chr5_1:31034426-31034445 Msa0727720:intron 20.0%
!! TATAATGGTATATATGTTTG+AGG - chr5_1:31034531-31034550 Msa0727720:intron 20.0%
!! TAATTACAATAACATAGAAG+CGG - chr5_1:31035263-31035282 Msa0727720:intron 20.0%
!!! AAATTACAGTTTTTTGACTT+GGG + chr5_1:31035423-31035442 None:intergenic 20.0%
!!! TATATTTTTCTCATTTGAAG+CGG + chr5_1:31035847-31035866 None:intergenic 20.0%
!! ATGATATAATGTGAAACTAT+AGG - chr5_1:31036149-31036168 Msa0727720:intron 20.0%
!! ACATATCCATTTAATAATCA+TGG + chr5_1:31036242-31036261 None:intergenic 20.0%
!!! TTAGCATATTTTTCTTTTCA+TGG + chr5_1:31036293-31036312 None:intergenic 20.0%
!! ACATCACACTTATAAAATTT+AGG + chr5_1:31036611-31036630 None:intergenic 20.0%
!!! TATTTTGAGACAACTATTTT+AGG - chr5_1:31038615-31038634 Msa0727720:intron 20.0%
!! ATGCCAATGAAAAATTAAAT+AGG - chr5_1:31038662-31038681 Msa0727720:intron 20.0%
!! TGCCAATGAAAAATTAAATA+GGG - chr5_1:31038663-31038682 Msa0727720:intron 20.0%
!! ACAAAAATAAAATGAAGCAT+AGG + chr5_1:31039246-31039265 None:intergenic 20.0%
! TGAAATTGGAATTGAGATTT+GGG + chr5_1:31033761-31033780 None:intergenic 25.0%
! TTGAAATTGGAATTGAGATT+TGG + chr5_1:31033762-31033781 None:intergenic 25.0%
! TATGCATAATTCATATTCTG+TGG - chr5_1:31034084-31034103 Msa0727720:intron 25.0%
!! ATTCTGTGGTGATATTTTAT+TGG - chr5_1:31034098-31034117 Msa0727720:intron 25.0%
! CCCAAAAATTCTACTATATA+TGG + chr5_1:31034140-31034159 None:intergenic 25.0%
!!! GGGTAATTTTTTCTAGAAAA+AGG - chr5_1:31034457-31034476 Msa0727720:intron 25.0%
! ACGAAAAAGTAGTAGTTAAT+TGG - chr5_1:31034484-31034503 Msa0727720:intron 25.0%
!! AGTTAATTGGTAGTTAACTT+TGG - chr5_1:31034497-31034516 Msa0727720:intron 25.0%
!!! GTTTTGTTGAGTTGTTATTT+AGG - chr5_1:31034556-31034575 Msa0727720:intron 25.0%
!! TATGATGATGTAGAAATCAT+AGG - chr5_1:31034736-31034755 Msa0727720:intron 25.0%
! AGAAATCGTTATGGTTTATA+TGG - chr5_1:31035105-31035124 Msa0727720:intron 25.0%
! GAAATCGTTATGGTTTATAT+GGG - chr5_1:31035106-31035125 Msa0727720:intron 25.0%
!!! AATTACAGTTTTTTGACTTG+GGG + chr5_1:31035422-31035441 None:intergenic 25.0%
!!! GAAATTACAGTTTTTTGACT+TGG + chr5_1:31035424-31035443 None:intergenic 25.0%
!! AAATTAGAGTTTTCAGAGTT+GGG + chr5_1:31035549-31035568 None:intergenic 25.0%
! GAAAACTCTAATTTCTGTAA+CGG - chr5_1:31035555-31035574 Msa0727720:intron 25.0%
!! ATTTCTGAAGAGAATTCTAA+TGG - chr5_1:31035750-31035769 Msa0727720:intron 25.0%
! TGATTTATACAAAAAAGCCA+TGG - chr5_1:31035896-31035915 Msa0727720:intron 25.0%
! TAAATACACATGCATTTACA+AGG + chr5_1:31036076-31036095 None:intergenic 25.0%
!!! ACTGAAGCATTTATAGTTTT+AGG - chr5_1:31036120-31036139 Msa0727720:intron 25.0%
! TCATCTCCATGATTATTAAA+TGG - chr5_1:31036233-31036252 Msa0727720:intron 25.0%
! TTATTAAATGGATATGTGTG+TGG - chr5_1:31036245-31036264 Msa0727720:intron 25.0%
! TATTAAATGGATATGTGTGT+GGG - chr5_1:31036246-31036265 Msa0727720:intron 25.0%
!! ATTTTTCATCTATCTGCTAA+AGG + chr5_1:31036475-31036494 None:intergenic 25.0%
! TTACATACATTAGTAATAGG+AGG + chr5_1:31036571-31036590 None:intergenic 25.0%
! CACTTACATACATTAGTAAT+AGG + chr5_1:31036574-31036593 None:intergenic 25.0%
! AAATTTAGGCCTAAATTACA+AGG + chr5_1:31036597-31036616 None:intergenic 25.0%
! AATATCCAGATGTATAACAT+TGG + chr5_1:31036881-31036900 None:intergenic 25.0%
! GTTATACATCTGGATATTAA+TGG - chr5_1:31036883-31036902 Msa0727720:intron 25.0%
!!! GGTGATGGATTTTTTTTAAA+AGG - chr5_1:31036972-31036991 Msa0727720:intron 25.0%
!! CTCTATCAATTTTCAATCAA+TGG + chr5_1:31037183-31037202 None:intergenic 25.0%
!! AACCATCAATTTTCAATCAA+TGG + chr5_1:31037248-31037267 None:intergenic 25.0%
! TACAAGAAACCATAACATAT+TGG + chr5_1:31037467-31037486 None:intergenic 25.0%
! TGGAATAAATTGAAGTCTAT+AGG + chr5_1:31037517-31037536 None:intergenic 25.0%
! AAAAATGCGCAAAAGAAAAA+AGG - chr5_1:31037667-31037686 Msa0727720:CDS 25.0%
! TGCAATTATCTCTTCTAAAT+TGG - chr5_1:31037769-31037788 Msa0727720:CDS 25.0%
!! TCTCCTTTATTTAAATTTGG+TGG - chr5_1:31037855-31037874 Msa0727720:CDS 25.0%
! AGTCCACCAAATTTAAATAA+AGG + chr5_1:31037861-31037880 None:intergenic 25.0%
!!! TTATTTAAATTTGGTGGACT+TGG - chr5_1:31037861-31037880 Msa0727720:CDS 25.0%
! TTAATTCTTTGTCCTGAATT+TGG + chr5_1:31038036-31038055 None:intergenic 25.0%
!! AGAATCTAAAATATGAAGTG+AGG + chr5_1:31038242-31038261 None:intergenic 25.0%
! AATGTTATGCTACAATCTTT+AGG - chr5_1:31038274-31038293 Msa0727720:CDS 25.0%
! TAGTTGTCTCAAAATATGAT+AGG + chr5_1:31038611-31038630 None:intergenic 25.0%
!!! TGCCCTATTTAATTTTTCAT+TGG + chr5_1:31038668-31038687 None:intergenic 25.0%
! AATGAAAAATTAAATAGGGC+AGG - chr5_1:31038667-31038686 Msa0727720:intron 25.0%
! ATGAAAAATTAAATAGGGCA+GGG - chr5_1:31038668-31038687 Msa0727720:intron 25.0%
! CGCTAGATAAATTATATAGT+CGG - chr5_1:31039175-31039194 Msa0727720:intron 25.0%
! ATTATCCAGCCATTAAATTT+GGG + chr5_1:31039308-31039327 None:intergenic 25.0%
! TATTATCCAGCCATTAAATT+TGG + chr5_1:31039309-31039328 None:intergenic 25.0%
! AAATTTAATGGCTGGATAAT+AGG - chr5_1:31039308-31039327 Msa0727720:five_prime_UTR 25.0%
! TAAAGAACAAGAACATGTAA+AGG - chr5_1:31039332-31039351 Msa0727720:five_prime_UTR 25.0%
!!! AAATGCCAAATTTTGAAGAT+GGG - chr5_1:31039368-31039387 Msa0727720:five_prime_UTR 25.0%
TGCTTGAATGTGTGAAAAAA+CGG - chr5_1:31033600-31033619 Msa0727720:three_prime_UTR 30.0%
!!! AGAAAGAGGGATTTTGAAAT+TGG + chr5_1:31033775-31033794 None:intergenic 30.0%
TTAACGGTTATGGAACAATT+GGG + chr5_1:31033866-31033885 None:intergenic 30.0%
ATTAACGGTTATGGAACAAT+TGG + chr5_1:31033867-31033886 None:intergenic 30.0%
ATAGCAATGATTAACGGTTA+TGG + chr5_1:31033876-31033895 None:intergenic 30.0%
! GTTATTGATTAGGGTTTCTT+AGG - chr5_1:31033915-31033934 Msa0727720:exon 30.0%
ATTAGGGTTTCTTAGGAATT+GGG - chr5_1:31033922-31033941 Msa0727720:CDS 30.0%
TGCTTTATGCTTTCAGAATA+TGG - chr5_1:31033968-31033987 Msa0727720:CDS 30.0%
GCTTTATGCTTTCAGAATAT+GGG - chr5_1:31033969-31033988 Msa0727720:CDS 30.0%
CTTTATGCTTTCAGAATATG+GGG - chr5_1:31033970-31033989 Msa0727720:CDS 30.0%
! CGTTATTCTTCTTGATTTTC+AGG - chr5_1:31033996-31034015 Msa0727720:CDS 30.0%
!!! AGTAGAATTTTTGGGTATTG+TGG - chr5_1:31034145-31034164 Msa0727720:intron 30.0%
GTTTACTTGATTTATACGAG+CGG - chr5_1:31034211-31034230 Msa0727720:intron 30.0%
AATAGAAAAATGCAGTAGAC+GGG + chr5_1:31034255-31034274 None:intergenic 30.0%
CAATAGAAAAATGCAGTAGA+CGG + chr5_1:31034256-31034275 None:intergenic 30.0%
AAAAGGAATTGGATTGCTAA+GGG - chr5_1:31034437-31034456 Msa0727720:intron 30.0%
TTGGAGTTGCCTTTATATAA+TGG - chr5_1:31034516-31034535 Msa0727720:intron 30.0%
!! AGAAAACCTTTTGTTTGAAG+AGG + chr5_1:31034935-31034954 None:intergenic 30.0%
GCTAAAGAAAGAAATCGTTA+TGG - chr5_1:31035096-31035115 Msa0727720:intron 30.0%
ATAACATAGAAGCGGAAAAT+AGG - chr5_1:31035271-31035290 Msa0727720:intron 30.0%
! AAAATAGGTCTGAAAAGCAT+GGG - chr5_1:31035286-31035305 Msa0727720:intron 30.0%
AGAAAGGACAACTAAGAAAA+AGG - chr5_1:31035386-31035405 Msa0727720:CDS 30.0%
AACTGTAATTTCTGTAACGA+TGG - chr5_1:31035432-31035451 Msa0727720:intron 30.0%
AATTTCTGTAACGATGGTAT+TGG - chr5_1:31035438-31035457 Msa0727720:intron 30.0%
TTTAAGGATGATATTCAGCA+TGG - chr5_1:31035504-31035523 Msa0727720:intron 30.0%
! AATTAGAGTTTTCAGAGTTG+GGG + chr5_1:31035548-31035567 None:intergenic 30.0%
! GAAATTAGAGTTTTCAGAGT+TGG + chr5_1:31035550-31035569 None:intergenic 30.0%
! TTTGATGAAAGGCATATCAA+TGG - chr5_1:31035780-31035799 Msa0727720:intron 30.0%
AAATTCAGGTGATTCACATA+TGG - chr5_1:31035952-31035971 Msa0727720:intron 30.0%
! TGTTATGCCTTTACATGTTT+TGG - chr5_1:31036003-31036022 Msa0727720:intron 30.0%
TAAAGAGCCAAAACATGTAA+AGG + chr5_1:31036013-31036032 None:intergenic 30.0%
TAGTTCTCTTACATGTTGAA+GGG - chr5_1:31036043-31036062 Msa0727720:intron 30.0%
ACTATAGGAGTTTCAAATCA+AGG - chr5_1:31036164-31036183 Msa0727720:intron 30.0%
GAATAACAATAGTAGATCTC+AGG + chr5_1:31036215-31036234 None:intergenic 30.0%
ACTAAATGAGAGAACAAAAC+AGG - chr5_1:31036358-31036377 Msa0727720:intron 30.0%
AACTTAGTGGAACTCAATTT+GGG + chr5_1:31036508-31036527 None:intergenic 30.0%
TAACTTAGTGGAACTCAATT+TGG + chr5_1:31036509-31036528 None:intergenic 30.0%
ATGTAAGTGCCTTGTAATTT+AGG - chr5_1:31036585-31036604 Msa0727720:intron 30.0%
AGAGGTAGTTACAGAAAATA+GGG + chr5_1:31036765-31036784 None:intergenic 30.0%
GATATTATTGATCTTCTCTG+TGG + chr5_1:31036800-31036819 None:intergenic 30.0%
AATTCCATATACGATCAGAA+TGG - chr5_1:31036849-31036868 Msa0727720:intron 30.0%
TAAACCATTCTGATCGTATA+TGG + chr5_1:31036856-31036875 None:intergenic 30.0%
ATATACGATCAGAATGGTTT+AGG - chr5_1:31036855-31036874 Msa0727720:intron 30.0%
AAAGGCTTCAAATTGAGTAA+TGG - chr5_1:31036990-31037009 Msa0727720:intron 30.0%
AAGGCTTCAAATTGAGTAAT+GGG - chr5_1:31036991-31037010 Msa0727720:intron 30.0%
AATACAGAACTTACGCATAA+AGG + chr5_1:31037040-31037059 None:intergenic 30.0%
! TTAGCTTCATTTTCTTTCAG+AGG + chr5_1:31037159-31037178 None:intergenic 30.0%
! AGCCATTGATTGAAAATTGA+TGG - chr5_1:31037178-31037197 Msa0727720:CDS 30.0%
! TGATTGAAAATTGATGGTTG+AGG - chr5_1:31037249-31037268 Msa0727720:CDS 30.0%
AATAACAAGAATGACATTGG+TGG + chr5_1:31037395-31037414 None:intergenic 30.0%
AGCAATAACAAGAATGACAT+TGG + chr5_1:31037398-31037417 None:intergenic 30.0%
TATGGCTAACCAATATGTTA+TGG - chr5_1:31037455-31037474 Msa0727720:CDS 30.0%
! AAAAATTTGCTCAAGCGAAA+GGG + chr5_1:31037602-31037621 None:intergenic 30.0%
GTATGCATATTCTCAGTTTA+CGG + chr5_1:31037628-31037647 None:intergenic 30.0%
CATGAAAGAAGACATTCAAA+TGG + chr5_1:31037937-31037956 None:intergenic 30.0%
CAAAGTTCTATGACTAAACT+AGG + chr5_1:31037972-31037991 None:intergenic 30.0%
AGGTAGAGAATGATATGATA+TGG + chr5_1:31038222-31038241 None:intergenic 30.0%
TTTCATGCAATAGTCATCAA+AGG + chr5_1:31038538-31038557 None:intergenic 30.0%
GATTGAATATGTTACACGTT+AGG + chr5_1:31038565-31038584 None:intergenic 30.0%
!! TCTTTTGAATGTGTGCTATT+AGG + chr5_1:31038730-31038749 None:intergenic 30.0%
! ATGTTACACATGAAGCATTT+GGG + chr5_1:31038867-31038886 None:intergenic 30.0%
ATGTTATTAAGCATGAACAC+AGG + chr5_1:31038989-31039008 None:intergenic 30.0%
! TGTCATCTGATCATCATTTT+AGG - chr5_1:31039012-31039031 Msa0727720:intron 30.0%
CTTAAGCATAGCATGTTTAT+AGG + chr5_1:31039047-31039066 None:intergenic 30.0%
GATAAATTATATAGTCGGAC+TGG - chr5_1:31039180-31039199 Msa0727720:intron 30.0%
TTATCCAGCCATTAAATTTG+GGG + chr5_1:31039307-31039326 None:intergenic 30.0%
CATGTAAAGGTTACAAACTT+AGG - chr5_1:31039345-31039364 Msa0727720:five_prime_UTR 30.0%
!! GAAATGCCAAATTTTGAAGA+TGG - chr5_1:31039367-31039386 Msa0727720:five_prime_UTR 30.0%
TACTGTCATATGAAAGAACT+AGG - chr5_1:31039431-31039450 Msa0727720:five_prime_UTR 30.0%
ACTGTCATATGAAAGAACTA+GGG - chr5_1:31039432-31039451 Msa0727720:five_prime_UTR 30.0%
GATTAGGGTTTCTTAGGAAT+TGG - chr5_1:31033921-31033940 Msa0727720:CDS 35.0%
GAAAAGGAATTGGATTGCTA+AGG - chr5_1:31034436-31034455 Msa0727720:intron 35.0%
TAGAAATCATAGGATCCAGT+GGG - chr5_1:31034746-31034765 Msa0727720:intron 35.0%
GTCGATGTTCTATCTTTACT+TGG + chr5_1:31034772-31034791 None:intergenic 35.0%
GACTGTGATGTCTATGAAAA+TGG - chr5_1:31035000-31035019 Msa0727720:CDS 35.0%
CGATTTCTTTCTTTAGCGTT+TGG + chr5_1:31035093-31035112 None:intergenic 35.0%
TGACATGCTCATTAAACACA+CGG + chr5_1:31035221-31035240 None:intergenic 35.0%
GAAAATAGGTCTGAAAAGCA+TGG - chr5_1:31035285-31035304 Msa0727720:intron 35.0%
CTACAACTAGGACCATATAA+AGG + chr5_1:31035318-31035337 None:intergenic 35.0%
ATATGGTCCTAGTTGTAGTA+AGG - chr5_1:31035320-31035339 Msa0727720:intron 35.0%
TGATTCTCCTTACTACAACT+AGG + chr5_1:31035330-31035349 None:intergenic 35.0%
ATGATATTCGGAATGGAGAA+AGG - chr5_1:31035370-31035389 Msa0727720:CDS 35.0%
! TGATGACGAGTCTACATTTA+AGG - chr5_1:31035488-31035507 Msa0727720:intron 35.0%
AAATTTATGAGCTCCAACCA+AGG - chr5_1:31035627-31035646 Msa0727720:intron 35.0%
ACAATGATGAATCTCCATTG+AGG - chr5_1:31035712-31035731 Msa0727720:intron 35.0%
TTGTCATCTTTACTCCTCAA+TGG + chr5_1:31035729-31035748 None:intergenic 35.0%
GATATGCCTTTCATCAAAAG+AGG + chr5_1:31035778-31035797 None:intergenic 35.0%
ATACAAAAAAGCCATGGAAG+AGG - chr5_1:31035902-31035921 Msa0727720:intron 35.0%
AATTTGCAATTGTCTCTGTC+TGG + chr5_1:31035937-31035956 None:intergenic 35.0%
ACAGAGACAATTGCAAATTC+AGG - chr5_1:31035938-31035957 Msa0727720:intron 35.0%
! CATGTAAAGGCATAACAGAA+AGG + chr5_1:31036000-31036019 None:intergenic 35.0%
!!! CATGTTTTGGCTCTTTAAAC+AGG - chr5_1:31036016-31036035 Msa0727720:intron 35.0%
GTAGTTCTCTTACATGTTGA+AGG - chr5_1:31036042-31036061 Msa0727720:intron 35.0%
ATAGGAGTTTCAAATCAAGG+CGG - chr5_1:31036167-31036186 Msa0727720:intron 35.0%
! GGCTCATAAGATTTTCAATG+AGG + chr5_1:31036454-31036473 None:intergenic 35.0%
GAACTCAATTTGGGAACAAT+AGG + chr5_1:31036499-31036518 None:intergenic 35.0%
!! TTTGTTGAGTGGTTTGGTTT+GGG - chr5_1:31036663-31036682 Msa0727720:intron 35.0%
!! ACTAACAAACCTCTAGGATA+TGG + chr5_1:31036701-31036720 None:intergenic 35.0%
GAGGTAGTTACAGAAAATAG+GGG + chr5_1:31036764-31036783 None:intergenic 35.0%
CAGAGGTAGTTACAGAAAAT+AGG + chr5_1:31036766-31036785 None:intergenic 35.0%
! TTTTCTGTAACTACCTCTGT+TGG - chr5_1:31036767-31036786 Msa0727720:intron 35.0%
TTAGGCCAATGTTATACATC+TGG - chr5_1:31036873-31036892 Msa0727720:intron 35.0%
! ATCTGGATATTAATGGACGA+AGG - chr5_1:31036890-31036909 Msa0727720:intron 35.0%
CTGAGTTTATGTTCTCATGA+AGG - chr5_1:31036951-31036970 Msa0727720:intron 35.0%
TTATGTTCTCATGAAGGTGA+TGG - chr5_1:31036957-31036976 Msa0727720:intron 35.0%
AGGCTTCAAATTGAGTAATG+GGG - chr5_1:31036992-31037011 Msa0727720:intron 35.0%
ATTGTACCTACAAGTCAGTA+TGG - chr5_1:31037057-31037076 Msa0727720:intron 35.0%
ACCAACTATTTCCACAGTTT+GGG + chr5_1:31037083-31037102 None:intergenic 35.0%
AACCAACTATTTCCACAGTT+TGG + chr5_1:31037084-31037103 None:intergenic 35.0%
! ATAGTTGGTTTCACCAACAT+AGG - chr5_1:31037094-31037113 Msa0727720:intron 35.0%
! TTGTAGAATTTTCCTCAGAC+TGG + chr5_1:31037124-31037143 None:intergenic 35.0%
ATTGATAGAGTGAACCATGA+CGG - chr5_1:31037193-31037212 Msa0727720:CDS 35.0%
AACAAAGCTTAGTGAAAGAG+CGG + chr5_1:31037568-31037587 None:intergenic 35.0%
! CAAAAATTTGCTCAAGCGAA+AGG + chr5_1:31037603-31037622 None:intergenic 35.0%
ACTGAGAATATGCATACTAC+AGG - chr5_1:31037631-31037650 Msa0727720:CDS 35.0%
TTCTTTCAATGTCCAGCAAT+CGG + chr5_1:31037703-31037722 None:intergenic 35.0%
TGTACTTTCATGCCAAATTC+AGG - chr5_1:31038021-31038040 Msa0727720:CDS 35.0%
AAAGAAGCAGCTCAATCATT+TGG - chr5_1:31038087-31038106 Msa0727720:CDS 35.0%
ATCAAAGGAAGGATGATTAG+TGG + chr5_1:31038523-31038542 None:intergenic 35.0%
ATGCAATAGTCATCAAAGGA+AGG + chr5_1:31038534-31038553 None:intergenic 35.0%
AAAAATTAAATAGGGCAGGG+AGG - chr5_1:31038671-31038690 Msa0727720:intron 35.0%
AGTTTACAATACCACCATAC+TGG + chr5_1:31038701-31038720 None:intergenic 35.0%
GTATGGTGGTATTGTAAACT+CGG - chr5_1:31038701-31038720 Msa0727720:intron 35.0%
AGACATTATCCTTACGAGAA+AGG - chr5_1:31038747-31038766 Msa0727720:intron 35.0%
GACATTATCCTTACGAGAAA+GGG - chr5_1:31038748-31038767 Msa0727720:intron 35.0%
AGAAGGGGATGGAATAAAAT+GGG - chr5_1:31038773-31038792 Msa0727720:intron 35.0%
! TGTTACACATGAAGCATTTG+GGG + chr5_1:31038866-31038885 None:intergenic 35.0%
! GATGTTACACATGAAGCATT+TGG + chr5_1:31038868-31038887 None:intergenic 35.0%
!!! AGAAGTTTTGTTCGACTTCA+TGG - chr5_1:31039152-31039171 Msa0727720:intron 35.0%
! TTATATAGTCGGACTGGATA+TGG - chr5_1:31039186-31039205 Msa0727720:intron 35.0%
CATTAAATTTGGGGGAAGAT+AGG + chr5_1:31039298-31039317 None:intergenic 35.0%
CTATCTTCCCCCAAATTTAA+TGG - chr5_1:31039296-31039315 Msa0727720:five_prime_UTR 35.0%
TATCCAGCCATTAAATTTGG+GGG + chr5_1:31039306-31039325 None:intergenic 35.0%
TACAGCCCATCTTCAAAATT+TGG + chr5_1:31039376-31039395 None:intergenic 35.0%
ATTCTTCCATGTCGTGTATT+TGG - chr5_1:31039398-31039417 Msa0727720:five_prime_UTR 35.0%
CTGTCATATGAAAGAACTAG+GGG - chr5_1:31039433-31039452 Msa0727720:five_prime_UTR 35.0%
AGCAAAAGGGATTGAAACAA+AGG - chr5_1:31039458-31039477 Msa0727720:five_prime_UTR 35.0%
GCAAAAGGGATTGAAACAAA+GGG - chr5_1:31039459-31039478 Msa0727720:five_prime_UTR 35.0%
! ACGATTTTGCTTCGAATCGA+AGG + chr5_1:31033699-31033718 None:intergenic 40.0%
GTTTCTTAGGAATTGGGACA+CGG - chr5_1:31033928-31033947 Msa0727720:CDS 40.0%
! AGGTGTGATTTTTGTGATGC+AGG - chr5_1:31034576-31034595 Msa0727720:intron 40.0%
CAGGTATAGATGCTTGGAAA+AGG - chr5_1:31034595-31034614 Msa0727720:intron 40.0%
GTAGAAATCATAGGATCCAG+TGG - chr5_1:31034745-31034764 Msa0727720:intron 40.0%
TCAATGCTGATAACACTCTG+AGG + chr5_1:31034796-31034815 None:intergenic 40.0%
! CAGTCTGGAATTGATGTTGA+TGG - chr5_1:31034886-31034905 Msa0727720:intron 40.0%
!! GGAATTGATGTTGATGGTGT+TGG - chr5_1:31034892-31034911 Msa0727720:intron 40.0%
TACGTATACAGATTTCCACG+CGG + chr5_1:31034971-31034990 None:intergenic 40.0%
AAAATGGTCCTGTTTCGAAG+AGG - chr5_1:31035016-31035035 Msa0727720:CDS 40.0%
!!! CTTTTTTGCCTCTTCGAAAC+AGG + chr5_1:31035027-31035046 None:intergenic 40.0%
TAGCGATTGTTCTGATTGTG+AGG - chr5_1:31035149-31035168 Msa0727720:intron 40.0%
AGGTCATAAAACGTGAACAG+TGG - chr5_1:31035169-31035188 Msa0727720:intron 40.0%
GGTCATAAAACGTGAACAGT+GGG - chr5_1:31035170-31035189 Msa0727720:intron 40.0%
AGAGGATACCGCAATGAAAA+AGG - chr5_1:31035194-31035213 Msa0727720:intron 40.0%
GAGGATACCGCAATGAAAAA+GGG - chr5_1:31035195-31035214 Msa0727720:intron 40.0%
AATGAGCATGTCAGTTCTTC+TGG - chr5_1:31035228-31035247 Msa0727720:intron 40.0%
CATGGGAAAGGTCCTTTATA+TGG - chr5_1:31035303-31035322 Msa0727720:intron 40.0%
GAGAATCAATCTTCCTTCAC+TGG - chr5_1:31035342-31035361 Msa0727720:CDS 40.0%
AATATCATCCTCACCAGTGA+AGG + chr5_1:31035358-31035377 None:intergenic 40.0%
TCACTGGTGAGGATGATATT+CGG - chr5_1:31035358-31035377 Msa0727720:CDS 40.0%
!! GTATTGGTTCTTCAAAGTCC+AGG - chr5_1:31035454-31035473 Msa0727720:intron 40.0%
TTCAAAGTCCAGGAATGAAC+TGG - chr5_1:31035464-31035483 Msa0727720:intron 40.0%
TCAAAGTCCAGGAATGAACT+GGG - chr5_1:31035465-31035484 Msa0727720:intron 40.0%
!! AATTTCTGTAACGGTGTTGC+TGG - chr5_1:31035564-31035583 Msa0727720:intron 40.0%
! GGTTCTTCAAGTTTGAAGCA+AGG - chr5_1:31035585-31035604 Msa0727720:intron 40.0%
! TCAAGTTTGAAGCAAGGACT+AGG - chr5_1:31035591-31035610 Msa0727720:intron 40.0%
TCTCCATTGAATGCACATGA+CGG - chr5_1:31035681-31035700 Msa0727720:intron 40.0%
! ATGGTGCCTCTTTTGATGAA+AGG - chr5_1:31035769-31035788 Msa0727720:intron 40.0%
AAAAGCCATGGAAGAGGAAA+TGG - chr5_1:31035908-31035927 Msa0727720:intron 40.0%
TACACATGCATTTACAAGGC+AGG + chr5_1:31036072-31036091 None:intergenic 40.0%
TAGGAGTTTCAAATCAAGGC+GGG - chr5_1:31036168-31036187 Msa0727720:intron 40.0%
GGTTTCTCCAATAGATACGA+GGG + chr5_1:31036433-31036452 None:intergenic 40.0%
AGGTTTCTCCAATAGATACG+AGG + chr5_1:31036434-31036453 None:intergenic 40.0%
CTTTGACGCATCTAACTTAG+TGG + chr5_1:31036521-31036540 None:intergenic 40.0%
!! GAACAAGAAAGCACCTCATT+CGG - chr5_1:31036640-31036659 Msa0727720:intron 40.0%
!! ATTCGGTTTGTTGAGTGGTT+TGG - chr5_1:31036657-31036676 Msa0727720:intron 40.0%
!! GTTTGTTGAGTGGTTTGGTT+TGG - chr5_1:31036662-31036681 Msa0727720:intron 40.0%
CAGGAAACTAACAAACCTCT+AGG + chr5_1:31036707-31036726 None:intergenic 40.0%
AAACTGACACAAACTGCAGA+TGG + chr5_1:31036736-31036755 None:intergenic 40.0%
! ATATTAATGGACGAAGGTGC+TGG - chr5_1:31036896-31036915 Msa0727720:intron 40.0%
GGCTTCAAATTGAGTAATGG+GGG - chr5_1:31036993-31037012 Msa0727720:intron 40.0%
TTGGTTTCACCAACATAGGA+TGG - chr5_1:31037098-31037117 Msa0727720:intron 40.0%
ACAATTATCACCCACTGCAA+TGG - chr5_1:31037278-31037297 Msa0727720:CDS 40.0%
CAATTATCACCCACTGCAAT+GGG - chr5_1:31037279-31037298 Msa0727720:CDS 40.0%
AATATGCATACTACAGGCAG+AGG - chr5_1:31037637-31037656 Msa0727720:CDS 40.0%
GATTGCTGGACATTGAAAGA+AGG - chr5_1:31037702-31037721 Msa0727720:CDS 40.0%
AGTGAGAGAGACACTAAAGA+AGG - chr5_1:31037745-31037764 Msa0727720:CDS 40.0%
TGAACACGAAAGATGAACTG+CGG - chr5_1:31038056-31038075 Msa0727720:CDS 40.0%
TAAACACAACTTGCCTCCTT+TGG + chr5_1:31038187-31038206 None:intergenic 40.0%
GAAGGAAAGCGATTTAATGG+TGG + chr5_1:31038470-31038489 None:intergenic 40.0%
AGCGAAGGAAAGCGATTTAA+TGG + chr5_1:31038473-31038492 None:intergenic 40.0%
GAGAAGGGGATGGAATAAAA+TGG - chr5_1:31038772-31038791 Msa0727720:intron 40.0%
AATGAACTCAACCGTGAGAA+GGG + chr5_1:31038901-31038920 None:intergenic 40.0%
AAATGAACTCAACCGTGAGA+AGG + chr5_1:31038902-31038921 None:intergenic 40.0%
GTAGCTCACAAATTTACTGC+AGG + chr5_1:31038935-31038954 None:intergenic 40.0%
AAGCATGAACACAGGACAAT+AGG + chr5_1:31038981-31039000 None:intergenic 40.0%
! TTTTTGATGCTCGATCTGGA+TGG + chr5_1:31039072-31039091 None:intergenic 40.0%
! TCAGTTTTTGATGCTCGATC+TGG + chr5_1:31039076-31039095 None:intergenic 40.0%
GTACTACCAAATACACGACA+TGG + chr5_1:31039407-31039426 None:intergenic 40.0%
GATGAAGTGCGAGAGAAAGA+GGG + chr5_1:31033788-31033807 None:intergenic 45.0%
AGATGAAGTGCGAGAGAAAG+AGG + chr5_1:31033789-31033808 None:intergenic 45.0%
CTTCTGCTTCTTCGCTTTCA+AGG - chr5_1:31033814-31033833 Msa0727720:three_prime_UTR 45.0%
TGCTTCTTCGCTTTCAAGGT+AGG - chr5_1:31033818-31033837 Msa0727720:three_prime_UTR 45.0%
GCTTCTTCGCTTTCAAGGTA+GGG - chr5_1:31033819-31033838 Msa0727720:three_prime_UTR 45.0%
GTGATGCAGGTATAGATGCT+TGG - chr5_1:31034589-31034608 Msa0727720:intron 45.0%
TCTTCCCCGAAGAACATGTT+CGG + chr5_1:31034645-31034664 None:intergenic 45.0%
GGGAAGAATGCATCCAAAAC+TGG - chr5_1:31034658-31034677 Msa0727720:intron 45.0%
GAATGCATCCAAAACTGGTC+AGG - chr5_1:31034663-31034682 Msa0727720:intron 45.0%
TCTATCTTTACTTGGCCCAC+TGG + chr5_1:31034764-31034783 None:intergenic 45.0%
! GATGATGATGACGACGATGA+TGG - chr5_1:31034847-31034866 Msa0727720:intron 45.0%
!! CGATGATGGAAGTGATGATG+TGG - chr5_1:31034861-31034880 Msa0727720:intron 45.0%
! AGTGATGATGTGGATCAGTC+TGG - chr5_1:31034871-31034890 Msa0727720:intron 45.0%
!! TGTTGATGGTGTTGGAGAGT+TGG - chr5_1:31034900-31034919 Msa0727720:CDS 45.0%
GCGATGCCTCTTCAAACAAA+AGG - chr5_1:31034926-31034945 Msa0727720:CDS 45.0%
AAGCTTCTGCTTCTAACGTG+CGG - chr5_1:31035052-31035071 Msa0727720:intron 45.0%
AAAACGTGAACAGTGGGAAG+AGG - chr5_1:31035176-31035195 Msa0727720:intron 45.0%
! AGGTCTGAAAAGCATGGGAA+AGG - chr5_1:31035291-31035310 Msa0727720:intron 45.0%
TCAATCTTCCTTCACTGGTG+AGG - chr5_1:31035347-31035366 Msa0727720:CDS 45.0%
GGTGAGGATGATATTCGGAA+TGG - chr5_1:31035363-31035382 Msa0727720:CDS 45.0%
!!! CAGTTTTTTGACTTGGGGCA+GGG + chr5_1:31035417-31035436 None:intergenic 45.0%
!!! ACAGTTTTTTGACTTGGGGC+AGG + chr5_1:31035418-31035437 None:intergenic 45.0%
TGTCCGTCATGTGCATTCAA+TGG + chr5_1:31035687-31035706 None:intergenic 45.0%
GCGAATACCCTCGTATCTAT+TGG - chr5_1:31036423-31036442 Msa0727720:intron 45.0%
!! ACCTCATTCGGTTTGTTGAG+TGG - chr5_1:31036652-31036671 Msa0727720:intron 45.0%
ACCACTCAACAAACCGAATG+AGG + chr5_1:31036656-31036675 None:intergenic 45.0%
AAACTGCAGATGGTAACTGC+AGG + chr5_1:31036726-31036745 None:intergenic 45.0%
CTGTGGAAACTTTCCAACAG+AGG + chr5_1:31036783-31036802 None:intergenic 45.0%
!! TTTGGGCCATACTGACTTGT+AGG + chr5_1:31037066-31037085 None:intergenic 45.0%
GCCCAAACTGTGGAAATAGT+TGG - chr5_1:31037079-31037098 Msa0727720:intron 45.0%
!! TCTTTCAGAGGTGCTCAACA+AGG + chr5_1:31037147-31037166 None:intergenic 45.0%
! ATAGAGTGAACCATGACGGA+AGG - chr5_1:31037197-31037216 Msa0727720:CDS 45.0%
GTTAGCAGTAAGCACTTGCA+TGG + chr5_1:31037537-31037556 None:intergenic 45.0%
GTGTCTCTCTCACTTCTTCA+AGG + chr5_1:31037739-31037758 None:intergenic 45.0%
AGTATATCCCCTCACTTCAG+AGG - chr5_1:31037893-31037912 Msa0727720:CDS 45.0%
!! ATCTAGCTCCTCTGAAGTGA+GGG + chr5_1:31037904-31037923 None:intergenic 45.0%
GAACACGAAAGATGAACTGC+GGG - chr5_1:31038057-31038076 Msa0727720:CDS 45.0%
TCGTGGCTTAGGAGTAAATG+TGG - chr5_1:31038141-31038160 Msa0727720:CDS 45.0%
CGTGGCTTAGGAGTAAATGT+GGG - chr5_1:31038142-31038161 Msa0727720:CDS 45.0%
GTGGCTTAGGAGTAAATGTG+GGG - chr5_1:31038143-31038162 Msa0727720:CDS 45.0%
TGGCTTAGGAGTAAATGTGG+GGG - chr5_1:31038144-31038163 Msa0727720:CDS 45.0%
AAGTCTTAGACCATCGCCAA+AGG - chr5_1:31038168-31038187 Msa0727720:CDS 45.0%
CTTACGAGAAAGGGAGAGAA+GGG - chr5_1:31038757-31038776 Msa0727720:intron 45.0%
TTACGAGAAAGGGAGAGAAG+GGG - chr5_1:31038758-31038777 Msa0727720:intron 45.0%
ATGAACTCAACCGTGAGAAG+GGG + chr5_1:31038900-31038919 None:intergenic 45.0%
AAAAACTGACATTCGCGAGC+AGG - chr5_1:31039087-31039106 Msa0727720:intron 45.0%
CTTCCCCCAAATTTAATGGC+TGG - chr5_1:31039300-31039319 Msa0727720:five_prime_UTR 45.0%
! AAGAACTAGGGGCTAGCAAA+AGG - chr5_1:31039444-31039463 Msa0727720:five_prime_UTR 45.0%
! AGAACTAGGGGCTAGCAAAA+GGG - chr5_1:31039445-31039464 Msa0727720:five_prime_UTR 45.0%
!!! AATTTTAATAAATGATAAAA+TGG - chr5_1:31037314-31037333 Msa0727720:CDS 5.0%
TCAGCCCCGAACATGTTCTT+CGG - chr5_1:31034636-31034655 Msa0727720:intron 50.0%
TTCCCCGAAGAACATGTTCG+GGG + chr5_1:31034643-31034662 None:intergenic 50.0%
CTTCCCCGAAGAACATGTTC+GGG + chr5_1:31034644-31034663 None:intergenic 50.0%
TCCAAAACTGGTCAGGCGAA+AGG - chr5_1:31034670-31034689 Msa0727720:intron 50.0%
CCAAAACTGGTCAGGCGAAA+GGG - chr5_1:31034671-31034690 Msa0727720:intron 50.0%
CAAAACTGGTCAGGCGAAAG+GGG - chr5_1:31034672-31034691 Msa0727720:intron 50.0%
GATTTCCACGCGGAACTTGA+GGG + chr5_1:31034961-31034980 None:intergenic 50.0%
AGATTTCCACGCGGAACTTG+AGG + chr5_1:31034962-31034981 None:intergenic 50.0%
!! CACACGGCCCTTTTTCATTG+CGG + chr5_1:31035205-31035224 None:intergenic 50.0%
GTCATCACCCAGTTCATTCC+TGG + chr5_1:31035475-31035494 None:intergenic 50.0%
! AGAGTTTTCAGAGTTGGGGC+AGG + chr5_1:31035544-31035563 None:intergenic 50.0%
CATGTCATGTGCACCTTGGT+TGG + chr5_1:31035643-31035662 None:intergenic 50.0%
CTTGCATGTCATGTGCACCT+TGG + chr5_1:31035647-31035666 None:intergenic 50.0%
! GGATGCCATTTCCTCTTCCA+TGG + chr5_1:31035916-31035935 None:intergenic 50.0%
!! GTGGTTTGGTTTGGGAGAGT+AGG - chr5_1:31036671-31036690 Msa0727720:intron 50.0%
GTAGGCTCTCCATATCCTAG+AGG - chr5_1:31036689-31036708 Msa0727720:intron 50.0%
!!! CGAAGGTGCTGGAGATGTTT+TGG - chr5_1:31036907-31036926 Msa0727720:intron 50.0%
! GGGGGATAGCGATTTAGAAC+AGG - chr5_1:31037011-31037030 Msa0727720:intron 50.0%
AGTCAGTATGGCCCAAACTG+TGG - chr5_1:31037069-31037088 Msa0727720:intron 50.0%
! TCAGACTGGCCATCCTATGT+TGG + chr5_1:31037110-31037129 None:intergenic 50.0%
AACATAGGATGGCCAGTCTG+AGG - chr5_1:31037109-31037128 Msa0727720:intron 50.0%
GTCACTATCTCCTTCCGTCA+TGG + chr5_1:31037210-31037229 None:intergenic 50.0%
!! TCTAGCTCCTCTGAAGTGAG+GGG + chr5_1:31037903-31037922 None:intergenic 50.0%
!! GATCTAGCTCCTCTGAAGTG+AGG + chr5_1:31037905-31037924 None:intergenic 50.0%
TCTTAGACCATCGCCAAAGG+AGG - chr5_1:31038171-31038190 Msa0727720:CDS 50.0%
CCTTCTCTCCCTTTCTCGTA+AGG + chr5_1:31038759-31038778 None:intergenic 50.0%
CCTTACGAGAAAGGGAGAGA+AGG - chr5_1:31038756-31038775 Msa0727720:intron 50.0%
CTGATGTCATCCCCTTCTCA+CGG - chr5_1:31038887-31038906 Msa0727720:intron 50.0%
CAAGCTCATCTCACGCATGA+AGG - chr5_1:31039129-31039148 Msa0727720:intron 50.0%
AAATGCAGTAGACGGGCACG+TGG + chr5_1:31034248-31034267 None:intergenic 55.0%
CAGCCCCGAACATGTTCTTC+GGG - chr5_1:31034637-31034656 Msa0727720:intron 55.0%
AGCCCCGAACATGTTCTTCG+GGG - chr5_1:31034638-31034657 Msa0727720:intron 55.0%
! CCCTTTCGCCTGACCAGTTT+TGG + chr5_1:31034674-31034693 None:intergenic 55.0%
AGGCTGAAGCCCATTGCAGT+GGG + chr5_1:31037291-31037310 None:intergenic 55.0%
TAGGCTGAAGCCCATTGCAG+TGG + chr5_1:31037292-31037311 None:intergenic 55.0%
GGCTGAGAATAGCCGATTGC+TGG - chr5_1:31037688-31037707 Msa0727720:CDS 55.0%
GATATGACCTCGCTGCTTCG+TGG - chr5_1:31038124-31038143 Msa0727720:CDS 55.0%
CAACTTGCCTCCTTTGGCGA+TGG + chr5_1:31038181-31038200 None:intergenic 55.0%
GCTTTCCTTCGCTGATGCTG+TGG - chr5_1:31038480-31038499 Msa0727720:exon 55.0%
AGCATCCACAGCATCAGCGA+AGG + chr5_1:31038488-31038507 None:intergenic 55.0%
AGGGAGGTGAACCAGTATGG+TGG - chr5_1:31038687-31038706 Msa0727720:intron 55.0%
GAGAAAGGGAGAGAAGGGGA+TGG - chr5_1:31038762-31038781 Msa0727720:intron 55.0%
TGACATTCGCGAGCAGGTTG+AGG - chr5_1:31039093-31039112 Msa0727720:intron 55.0%
CTATGCCCTCAAGTTCCGCG+TGG - chr5_1:31034953-31034972 Msa0727720:CDS 60.0%
ACCTCGCTGCTTCGTGGCTT+AGG - chr5_1:31038130-31038149 Msa0727720:CDS 60.0%
TCCTAAGCCACGAAGCAGCG+AGG + chr5_1:31038134-31038153 None:intergenic 60.0%
GGCAGGGAGGTGAACCAGTA+TGG - chr5_1:31038684-31038703 Msa0727720:intron 60.0%
! TCGCGAGCAGGTTGAGGCTG+AGG - chr5_1:31039099-31039118 Msa0727720:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 31033598 31039501 31033598 ID=Msa0727720;Name=Msa0727720
chr5_1 mRNA 31033598 31039501 31033598 ID=Msa0727720-mRNA-1;Parent=Msa0727720;Name=Msa0727720-mRNA-1;_AED=0.10;_eAED=0.10;_QI=244|0.75|0.8|1|0.5|0.4|5|321|591
chr5_1 exon 31033598 31034065 31033598 ID=Msa0727720-mRNA-1:exon:10285;Parent=Msa0727720-mRNA-1
chr5_1 exon 31034898 31035056 31034898 ID=Msa0727720-mRNA-1:exon:10284;Parent=Msa0727720-mRNA-1
chr5_1 exon 31035333 31035446 31035333 ID=Msa0727720-mRNA-1:exon:10283;Parent=Msa0727720-mRNA-1
chr5_1 exon 31037140 31038501 31037140 ID=Msa0727720-mRNA-1:exon:10282;Parent=Msa0727720-mRNA-1
chr5_1 exon 31039264 31039501 31039264 ID=Msa0727720-mRNA-1:exon:10281;Parent=Msa0727720-mRNA-1
chr5_1 five_prime_UTR 31039264 31039501 31039264 ID=Msa0727720-mRNA-1:five_prime_utr;Parent=Msa0727720-mRNA-1
chr5_1 five_prime_UTR 31038496 31038501 31038496 ID=Msa0727720-mRNA-1:five_prime_utr;Parent=Msa0727720-mRNA-1
chr5_1 CDS 31037140 31038495 31037140 ID=Msa0727720-mRNA-1:cds;Parent=Msa0727720-mRNA-1
chr5_1 CDS 31035333 31035446 31035333 ID=Msa0727720-mRNA-1:cds;Parent=Msa0727720-mRNA-1
chr5_1 CDS 31034898 31035056 31034898 ID=Msa0727720-mRNA-1:cds;Parent=Msa0727720-mRNA-1
chr5_1 CDS 31033919 31034065 31033919 ID=Msa0727720-mRNA-1:cds;Parent=Msa0727720-mRNA-1
chr5_1 three_prime_UTR 31033598 31033918 31033598 ID=Msa0727720-mRNA-1:three_prime_utr;Parent=Msa0727720-mRNA-1
Gene Sequence

>Msa0727720

ATGCTTGGAAAAGGTGTTTCGAGAGAACATTTTCAGCCCCGAACATGTTCTTCGGGGAAGAATGCATCCAAAACTGGTCAGGCGAAAGGGGATTTAGAGAATATTGTTTTCATTGACTTAGATAATGATCAATATGATGATGTAGAAATCATAGGATCCAGTGGGCCAAGTAAAGATAGAACATCGACGCCTCAGAGTGTTATCAGCATTGATGACGACGACGACGACGACGATGACGATGATGATGATGATGACGACGATGATGGAAGTGATGATGTGGATCAGTCTGGAATTGATGTTGATGGTGTTGGAGAGTTGGATAGCGATGCCTCTTCAAACAAAAGGTTTTCTATGCCCTCAAGTTCCGCGTGGAAATCTGTATACGTAGATGTTGATGACTGTGATGTCTATGAAAATGGTCCTGTTTCGAAGAGGCAAAAAAGTAATAAAGCTTCTGCTTCTAACGTGCGGAAGAAAGTATTCTCTCCAAACGCTAAAGAAAGAAATCGTTATGGTTTATATGGGTCTGAAAGTGACTCATCTGATAGCGATTGTTCTGATTGTGAGGTCATAAAACGTGAACAGTGGGAAGAGGATACCGCAATGAAAAAGGGCCGTGTGTTTAATGAGCATGTCAGTTCTTCTGGTTTACATACAAATAATTACAATAACATAGAAGCGGAAAATAGGTCTGAAAAGCATGGGAAAGGTCCTTTATATGGTCCTAGTTGTAGTAAGGAGAATCAATCTTCCTTCACTGGTGAGGATGATATTCGGAATGGAGAAAGGACAACTAAGAAAAAGGTTAATCCCTGCCCCAAGTCAAAAAACTGTAATTTCTGTAACGATGGTATTGGTTCTTCAAAGTCCAGGAATGAACTGGGTGATGACGAGTCTACATTTAAGGATGATATTCAGCATGGAGAAAAAGTTTCTGCCTGCCCCAACTCTGAAAACTCTAATTTCTGTAACGGTGTTGCTGGTTCTTCAAGTTTGAAGCAAGGACTAGGTGATAAAGAGTCTAAATTTATGAGCTCCAACCAAGGTGCACATGACATGCAAGTTGACGATGATGAATCTCCATTGAATGCACATGACGGACAAGTTGACAATGATGAATCTCCATTGAGGAGTAAAGATGACAATATTTCTGAAGAGAATTCTAATGGTGCCTCTTTTGATGAAAGGCATATCAATGGTCATGATGAGCTTGCTTTACATACTCAAGATGCTGATCTTACCGCTTCAAATGAGAAAAATATAATTAATCAAAAAGAAAAGCATAAGCAAACTGATTTATACAAAAAAGCCATGGAAGAGGAAATGGCATCCAGACAGAGACAATTGCAAATTCAGGCAGAGGAAGCACAAAAAATGCGCAAAAGAAAAAAGGCTGAGAATAGCCGATTGCTGGACATTGAAAGAAGGTCAAAAGAACGCCTTGAAGAAGTGAGAGAGACACTAAAGAAGGACAAAGAATTAATGAACACGAAAGATGAACTGCGGGTCGAAATAAAGAAGCAGCTCAATCATTTGGAGAATCAATGCATAGATATGACCTCGCTGCTTCGTGGCTTAGGAGTAAATGTGGGGGCAAGTCTTAGACCATCGCCAAAGGAGGCAAGTTGTGTGCATACTGCCTATAAACATGCTATGCTTAAGTTCCATCCAGATCGAGCATCAAAAACTGACATTCGCGAGCAGGTTGAGGCTGAGGAGACGTGCAAGCTCATCTCACGCATGAAGGAGAAGTTTTGTTCGACTTCATGGCGCTAG
Protein sequence

>Msa0727720

MLGKGVSREHFQPRTCSSGKNASKTGQAKGDLENIVFIDLDNDQYDDVEIIGSSGPSKDRTSTPQSVISIDDDDDDDDDDDDDDDDDDGSDDVDQSGIDVDGVGELDSDASSNKRFSMPSSSAWKSVYVDVDDCDVYENGPVSKRQKSNKASASNVRKKVFSPNAKERNRYGLYGSESDSSDSDCSDCEVIKREQWEEDTAMKKGRVFNEHVSSSGLHTNNYNNIEAENRSEKHGKGPLYGPSCSKENQSSFTGEDDIRNGERTTKKKVNPCPKSKNCNFCNDGIGSSKSRNELGDDESTFKDDIQHGEKVSACPNSENSNFCNGVAGSSSLKQGLGDKESKFMSSNQGAHDMQVDDDESPLNAHDGQVDNDESPLRSKDDNISEENSNGASFDERHINGHDELALHTQDADLTASNEKNIINQKEKHKQTDLYKKAMEEEMASRQRQLQIQAEEAQKMRKRKKAENSRLLDIERRSKERLEEVRETLKKDKELMNTKDELRVEIKKQLNHLENQCIDMTSLLRGLGVNVGASLRPSPKEASCVHTAYKHAMLKFHPDRASKTDIREQVEAEETCKLISRMKEKFCSTSWR*