AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1068480


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1068480 MtrunA17_Chr7g0249931 92.982 114 8 0 19 132 246 359 7.35e-73 226
Msa1068480 MtrunA17_Chr7g0250021 77.064 109 25 0 19 127 218 326 7.38e-60 188
Msa1068480 MtrunA17_Chr7g0249961 63.934 122 43 1 18 138 21 142 1.64e-49 160
Msa1068480 MtrunA17_Chr7g0249981 63.636 121 43 1 19 138 229 349 1.73e-48 163
Msa1068480 MtrunA17_Chr7g0250001 63.636 121 43 1 19 138 210 330 2.15e-47 159
Msa1068480 MtrunA17_Chr6g0464871 57.391 115 46 2 20 132 230 343 8.37e-38 134
Msa1068480 MtrunA17_Chr6g0464841 54.472 123 53 2 19 139 217 338 3.15e-37 132
Msa1068480 MtrunA17_Chr6g0464881 53.913 115 50 2 20 132 218 331 4.34e-37 132
Msa1068480 MtrunA17_Chr6g0464921 54.310 116 49 3 20 132 218 332 7.06e-36 129
Msa1068480 MtrunA17_Chr6g0464861 51.639 122 56 2 20 139 217 337 4.15e-34 124
Msa1068480 MtrunA17_Chr5g0414331 47.573 103 51 2 33 132 228 330 3.29e-23 94.4
Msa1068480 MtrunA17_Chr5g0414321 46.602 103 52 2 33 132 226 328 6.39e-22 90.9
Msa1068480 MtrunA17_Chr8g0380531 40.678 118 65 3 19 132 218 334 1.03e-17 79.0
Msa1068480 MtrunA17_Chr8g0377921 40.594 101 56 1 31 127 229 329 3.05e-17 77.8
Msa1068480 MtrunA17_Chr3g0122001 43.750 80 42 1 35 111 717 796 2.01e-16 75.5
Msa1068480 MtrunA17_Chr3g0122001 42.500 80 43 1 35 111 237 316 2.32e-15 72.4
Msa1068480 MtrunA17_Chr3g0121991 42.500 80 43 1 35 111 310 389 1.69e-15 72.8
Msa1068480 MtrunA17_Chr1g0207981 39.806 103 56 3 35 132 248 349 5.03e-15 71.2
Msa1068480 MtrunA17_Chr8g0377941 42.708 96 42 3 35 127 248 333 3.34e-14 68.9
Msa1068480 MtrunA17_Chr1g0161351 38.655 119 60 4 23 132 258 372 5.62e-14 68.2
Msa1068480 MtrunA17_Chr1g0207861 42.202 109 50 5 33 132 239 343 1.85e-13 67.0
Msa1068480 MtrunA17_Chr7g0243611 35.537 121 67 3 26 139 101 217 2.91e-13 65.9
Msa1068480 MtrunA17_Chr3g0098111 40.367 109 52 4 33 132 240 344 3.84e-13 65.9
Msa1068480 MtrunA17_Chr7g0243801 41.379 87 42 3 26 111 236 314 5.78e-13 65.5
Msa1068480 MtrunA17_Chr7g0243771 41.379 87 42 3 26 111 235 313 6.07e-13 65.5
Msa1068480 MtrunA17_Chr5g0401721 33.019 106 64 2 34 132 242 347 7.96e-13 65.1
Msa1068480 MtrunA17_Chr7g0243631 37.113 97 57 1 26 122 242 334 7.98e-13 65.1
Msa1068480 MtrunA17_Chr1g0207931 40.367 109 52 4 33 132 268 372 1.41e-12 64.3
Msa1068480 MtrunA17_Chr7g0243681 37.190 121 60 4 26 139 240 351 1.64e-12 63.9
Msa1068480 MtrunA17_Chr8g0365811 38.318 107 52 4 18 122 713 807 1.76e-12 64.3
Msa1068480 MtrunA17_Chr8g0365811 34.653 101 56 3 18 116 231 323 4.22e-12 63.2
Msa1068480 MtrunA17_Chr1g0207951 40.777 103 52 4 33 132 887 983 2.67e-12 63.5
Msa1068480 MtrunA17_Chr1g0207951 46.377 69 33 2 65 132 382 447 8.97e-11 59.3
Msa1068480 MtrunA17_Chr5g0442601 42.308 52 30 0 60 111 657 708 2.90e-12 63.5
Msa1068480 MtrunA17_Chr7g0235051 37.143 105 59 2 35 132 215 319 3.48e-12 63.2
Msa1068480 MtrunA17_Chr5g0393591 51.852 54 26 0 62 115 252 305 3.77e-12 63.2
Msa1068480 MtrunA17_Chr7g0243781 39.535 86 40 3 26 111 238 311 3.80e-12 63.2
Msa1068480 MtrunA17_Chr4g0044481 41.026 78 42 2 59 132 259 336 3.98e-12 62.8
Msa1068480 MtrunA17_Chr8g0361371 39.130 92 38 2 63 136 254 345 4.20e-12 62.8
Msa1068480 MtrunA17_Chr7g0243811 39.535 86 40 3 26 111 213 286 4.43e-12 62.8
Msa1068480 MtrunA17_Chr4g0053501 41.975 81 37 2 62 132 261 341 4.77e-12 62.8
Msa1068480 MtrunA17_Chr8g0334831 53.061 49 23 0 63 111 253 301 4.87e-12 62.8
Msa1068480 MtrunA17_Chr7g0243711 38.961 77 47 0 35 111 78 154 5.30e-12 62.4
Msa1068480 MtrunA17_Chr4g0053321 41.975 81 37 2 62 132 276 356 5.46e-12 62.8
Msa1068480 MtrunA17_Chr5g0442521 32.877 73 49 0 39 111 235 307 5.94e-12 62.4
Msa1068480 MtrunA17_Chr7g0243951 37.363 91 52 2 26 115 240 326 7.19e-12 62.4
Msa1068480 MtrunA17_Chr7g0243861 35.922 103 61 2 26 127 243 341 1.21e-11 61.6
Msa1068480 MtrunA17_Chr5g0401851 50.980 51 25 0 65 115 276 326 1.58e-11 61.2
Msa1068480 MtrunA17_Chr5g0409591 33.962 106 63 3 35 135 240 343 2.45e-11 60.8
Msa1068480 MtrunA17_Chr7g0243871 38.211 123 54 8 19 132 229 338 3.20e-11 60.5
Msa1068480 MtrunA17_Chr4g0042891 38.532 109 48 4 34 132 243 342 6.16e-11 59.7
Msa1068480 MtrunA17_Chr7g0243761 35.088 114 63 3 26 132 240 349 8.27e-11 59.3
Msa1068480 MtrunA17_Chr1g0207971 38.095 105 54 5 33 132 232 330 8.55e-11 59.3
Msa1068480 MtrunA17_Chr8g0366391 38.750 80 45 2 57 132 269 348 9.57e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1068480 AT1G05680.1 42.735 117 63 3 19 132 214 329 3.72e-24 97.4
Msa1068480 AT2G31750.1 43.103 116 62 3 20 132 215 329 8.07e-24 96.7
Msa1068480 AT2G31750.2 43.103 116 62 3 20 132 215 329 8.84e-24 96.7
Msa1068480 AT1G05675.1 42.735 117 63 3 19 132 214 329 2.57e-23 95.1
Msa1068480 AT2G31790.1 40.336 119 64 4 20 132 216 333 1.02e-22 93.6
Msa1068480 AT2G43820.1 47.826 92 43 3 34 122 221 310 9.56e-20 85.1
Msa1068480 AT2G43840.1 43.478 92 47 2 34 122 221 310 1.64e-19 84.3
Msa1068480 AT2G43840.2 43.478 92 47 2 34 122 221 310 1.69e-19 84.3
Msa1068480 AT1G24100.1 42.157 102 57 1 35 134 234 335 4.00e-19 83.6
Msa1068480 AT4G15480.1 37.864 103 54 3 33 129 250 348 1.41e-13 67.4
Msa1068480 AT2G28080.1 32.000 125 68 5 18 132 235 352 2.83e-12 63.9
Msa1068480 AT1G07250.1 33.645 107 60 3 32 131 244 346 4.31e-12 63.2
Msa1068480 AT3G16520.3 33.043 115 61 4 32 136 234 342 8.43e-12 62.4
Msa1068480 AT3G16520.2 32.432 111 59 4 32 132 234 338 1.02e-11 62.0
Msa1068480 AT3G16520.1 32.432 111 59 4 32 132 234 338 1.06e-11 62.0
Msa1068480 AT2G29710.1 40.541 74 40 2 62 131 262 335 2.58e-11 60.8
Msa1068480 AT1G07260.1 31.967 122 71 4 18 131 228 345 5.92e-11 60.1
Msa1068480 AT4G34135.1 36.250 80 47 2 57 132 272 351 5.99e-11 60.1
Msa1068480 AT4G15500.1 35.294 102 56 3 31 126 237 334 6.57e-11 59.7
Msa1068480 AT3G21560.1 37.037 81 51 0 34 114 246 326 8.60e-11 59.7

Find 18 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGTTTCATTTGGGAGTA+TGG 0.241418 7_2:-63424442 Msa1068480:CDS
TCTGTTGTTTATGTTTCATT+TGG 0.269067 7_2:-63424450 Msa1068480:CDS
TGAAGATTTGGTCCAACTTT+AGG 0.275771 7_2:-63424596 Msa1068480:CDS
TTCTGATAGAAAACTGCTTC+TGG 0.320806 7_2:+63424238 None:intergenic
CTGTTGTTTATGTTTCATTT+GGG 0.362285 7_2:-63424449 Msa1068480:CDS
CTGATTGGACAGTGAAGATT+TGG 0.396882 7_2:-63424608 Msa1068480:CDS
GGCTAAATAATAAGCCAAAA+AGG 0.404240 7_2:-63424473 Msa1068480:CDS
ATGCTTTGTAGGTGGCTGAT+TGG 0.463116 7_2:-63424623 Msa1068480:intron
ACTCAATGAGGAACAAATAG+AGG 0.483111 7_2:-63424415 Msa1068480:CDS
TTTGAAAAGAAATCAGAGAA+TGG 0.483547 7_2:-63424309 Msa1068480:CDS
GCACATTGTTTGAAAGATTG+TGG 0.490834 7_2:-63424387 Msa1068480:CDS
AGGCCCTACGGTCCTAAAGT+TGG 0.523956 7_2:+63424584 None:intergenic
TGGTCCAACTTTAGGACCGT+AGG 0.528491 7_2:-63424588 Msa1068480:CDS
TGGGAGCTTAATCTCTTCAG+AGG 0.533679 7_2:+63424338 None:intergenic
GGTCCAACTTTAGGACCGTA+GGG 0.573039 7_2:-63424587 Msa1068480:CDS
AAAGTGATGAGAGTATAGAA+TGG 0.588142 7_2:-63424494 Msa1068480:CDS
ATATGGTAGGCAAGGCCCTA+CGG 0.606429 7_2:+63424572 None:intergenic
GAGTATGGCATCACTCAATG+AGG 0.672707 7_2:-63424427 Msa1068480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACAAACAATTTAAATATA+TGG + chr7_2:63424393-63424412 None:intergenic 10.0%
!!! ATTTCTTTTCAAAGTTTTTT+GGG + chr7_2:63424811-63424830 None:intergenic 15.0%
!!! TATTTTTAGGTCCAAAATTA+GGG - chr7_2:63424276-63424295 Msa1068480:CDS 20.0%
!! TATCTAAAAATGTATATGGT+AGG + chr7_2:63424571-63424590 None:intergenic 20.0%
!!! GATTTCTTTTCAAAGTTTTT+TGG + chr7_2:63424812-63424831 None:intergenic 20.0%
!! AGAATTGAGCAAGTATTTTT+AGG - chr7_2:63424263-63424282 Msa1068480:CDS 25.0%
!!! GTATTTTTAGGTCCAAAATT+AGG - chr7_2:63424275-63424294 Msa1068480:CDS 25.0%
!! TACATGTTATACCCTAATTT+TGG + chr7_2:63424290-63424309 None:intergenic 25.0%
!!! GTCTTTAGTTATGCTATTTT+TGG + chr7_2:63424426-63424445 None:intergenic 25.0%
! AATGACTATAAATGCTTTGT+AGG - chr7_2:63424493-63424512 Msa1068480:CDS 25.0%
! CGCTTATCTAAAAATGTATA+TGG + chr7_2:63424575-63424594 None:intergenic 25.0%
!! TCTGTTGTTTATGTTTCATT+TGG - chr7_2:63424677-63424696 Msa1068480:intron 25.0%
!! CTGTTGTTTATGTTTCATTT+GGG - chr7_2:63424678-63424697 Msa1068480:intron 25.0%
!!! ATTGTGGAAGTTACTTTTTA+TGG - chr7_2:63424756-63424775 Msa1068480:intron 25.0%
!!! TTGTGGAAGTTACTTTTTAT+GGG - chr7_2:63424757-63424776 Msa1068480:intron 25.0%
! TTTGAAAAGAAATCAGAGAA+TGG - chr7_2:63424818-63424837 Msa1068480:intron 25.0%
GTTAATCACATGTAAAATCG+TGG - chr7_2:63424310-63424329 Msa1068480:CDS 30.0%
TTACAAAAGCAACACAAAAG+TGG + chr7_2:63424461-63424480 None:intergenic 30.0%
AAAAATGTATATGGTAGGCA+AGG + chr7_2:63424566-63424585 None:intergenic 30.0%
! AAAGTGATGAGAGTATAGAA+TGG - chr7_2:63424633-63424652 Msa1068480:intron 30.0%
GGCTAAATAATAAGCCAAAA+AGG - chr7_2:63424654-63424673 Msa1068480:intron 30.0%
! ACATAAACAACAGACCTTTT+TGG + chr7_2:63424671-63424690 None:intergenic 30.0%
TTATGTTTCATTTGGGAGTA+TGG - chr7_2:63424685-63424704 Msa1068480:intron 30.0%
ACACAAAAGTGGTATGCTTA+CGG + chr7_2:63424450-63424469 None:intergenic 35.0%
GACTATAAATGCTTTGTAGG+TGG - chr7_2:63424496-63424515 Msa1068480:CDS 35.0%
! TGAAGATTTGGTCCAACTTT+AGG - chr7_2:63424531-63424550 Msa1068480:CDS 35.0%
ACTCAATGAGGAACAAATAG+AGG - chr7_2:63424712-63424731 Msa1068480:intron 35.0%
GCACATTGTTTGAAAGATTG+TGG - chr7_2:63424740-63424759 Msa1068480:intron 35.0%
TTCTGATAGAAAACTGCTTC+TGG + chr7_2:63424892-63424911 None:intergenic 35.0%
CTGATTGGACAGTGAAGATT+TGG - chr7_2:63424519-63424538 Msa1068480:CDS 40.0%
ATGCTTTGTAGGTGGCTGAT+TGG - chr7_2:63424504-63424523 Msa1068480:CDS 45.0%
GAGTATGGCATCACTCAATG+AGG - chr7_2:63424700-63424719 Msa1068480:intron 45.0%
TGGGAGCTTAATCTCTTCAG+AGG + chr7_2:63424792-63424811 None:intergenic 45.0%
!! TGGTCCAACTTTAGGACCGT+AGG - chr7_2:63424539-63424558 Msa1068480:CDS 50.0%
!! GGTCCAACTTTAGGACCGTA+GGG - chr7_2:63424540-63424559 Msa1068480:CDS 50.0%
ATATGGTAGGCAAGGCCCTA+CGG + chr7_2:63424558-63424577 None:intergenic 50.0%
AGGCCCTACGGTCCTAAAGT+TGG + chr7_2:63424546-63424565 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 63424215 63424934 63424215 ID=Msa1068480;Name=Msa1068480
chr7_2 mRNA 63424215 63424934 63424215 ID=Msa1068480-mRNA-1;Parent=Msa1068480;Name=Msa1068480-mRNA-1;_AED=0.65;_eAED=0.65;_QI=0|0|0|0.5|1|1|2|0|159
chr7_2 exon 63424875 63424934 63424875 ID=Msa1068480-mRNA-1:exon:13166;Parent=Msa1068480-mRNA-1
chr7_2 exon 63424215 63424634 63424215 ID=Msa1068480-mRNA-1:exon:13165;Parent=Msa1068480-mRNA-1
chr7_2 CDS 63424875 63424934 63424875 ID=Msa1068480-mRNA-1:cds;Parent=Msa1068480-mRNA-1
chr7_2 CDS 63424215 63424634 63424215 ID=Msa1068480-mRNA-1:cds;Parent=Msa1068480-mRNA-1
Gene Sequence

>Msa1068480

ATGCAATTGATCAGAGACAGTTCAGATGACTCAAGATGCAAGAAAAGAAGAATTGAGCAAGTGGCTGATTGGACAGTGAAGATTTGGTCCAACTTTAGGACCGTAGGGCCTTGCCTACCATATACATTTTTAGATAAGCGAGTTAAAGATGATGAAGATTATGTTATTGCACAATTAAAAAGTGATGAGAGTATAGAATGGCTAAATAATAAGCCAAAAAGGTCTGTTGTTTATGTTTCATTTGGGAGTATGGCATCACTCAATGAGGAACAAATAGAGGAAGTAGCACATTGTTTGAAAGATTGTGGAAGTTACTTTTTATGGGTAGTGAAAACCTCTGAAGAGATTAAGCTCCCAAAAAACTTTGAAAAGAAATCAGAGAATGGCTTAGTTGTTGTTAGCGCAGCAAGCTTGTTGAACAAGCAATCAGAAAACCAGAAGCAGTTTTCTATCAGAAGCAGTTTCTATCAGAAGCAGTAA

Protein sequence

>Msa1068480

MQLIRDSSDDSRCKKRRIEQVADWTVKIWSNFRTVGPCLPYTFLDKRVKDDEDYVIAQLKSDESIEWLNNKPKRSVVYVSFGSMASLNEEQIEEVAHCLKDCGSYFLWVVKTSEEIKLPKNFEKKSENGLVVVSAASLLNKQSENQKQFSIRSSFYQKQ*