AlfalfaGEDB | Msa0125530

M. sativa cultivar zhongmu-4 / Msa0125530

KEGG: K00574
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0008825 GO:0009987 GO:0016740 GO:0016741 GO:0030258 GO:0032259 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0071704
PFAM: PF02353.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0125530	sp\|O53732\|UFAA1_MYCTU	39.041	146	89	0	6	151	280	425	6.84e-29	113

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0125530	A0A072VM29	98.113	159	3	0	6	164	702	860	2.25e-107	335

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001460	Msa0125530	0.823578	1.284472e-53	-8.615850e-47
Msa0004350	Msa0125530	0.824732	6.880885e-54	-8.615850e-47
Msa0004610	Msa0125530	0.812726	3.667285e-51	-8.615850e-47
Msa0006700	Msa0125530	0.801351	9.380575e-49	-8.615850e-47
Msa0008850	Msa0125530	0.815578	8.604207e-52	-8.615850e-47
Msa0012460	Msa0125530	0.814447	1.533189e-51	-8.615850e-47
Msa0017120	Msa0125530	0.824882	6.342877e-54	-8.615850e-47
Msa0020630	Msa0125530	0.857580	1.482069e-62	-8.615850e-47
Msa0024680	Msa0125530	0.810190	1.304009e-50	-8.615850e-47
Msa0027540	Msa0125530	0.815341	9.714840e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0125530	MtrunA17_Chr1g0186361	98.113	159	3	0	6	164	702	860	4.33e-111	335
Msa0125530	MtrunA17_Chr1g0168291	70.482	166	24	1	6	146	264	429	1.45e-78	241

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0125530	AT3G23510.1	74.214	159	41	0	6	164	703	861	1.73e-87	273
Msa0125530	AT3G23530.1	74.843	159	40	0	6	164	703	861	2.11e-87	273
Msa0125530	AT3G23470.1	69.182	159	47	1	6	162	295	453	6.77e-82	249
Msa0125530	AT3G23470.3	69.182	159	47	1	6	162	322	480	2.68e-81	248
Msa0125530	AT3G23470.2	69.182	159	47	1	6	162	322	480	2.68e-81	248
Msa0125530	AT3G23480.3	69.182	159	47	1	6	162	235	393	2.82e-79	242
Msa0125530	AT3G23480.2	69.182	159	47	1	6	162	272	430	4.57e-79	243
Msa0125530	AT3G23480.1	66.443	149	50	0	6	154	293	441	5.02e-72	223
Msa0125530	AT3G23460.1	72.368	76	21	0	83	158	209	284	2.33e-37	130

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Find 180 sgRNAs with CRISPR-Local

Find 492 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTCATGGAAAGGCAG+AGG	0.580802	1_3:+53993663	Msa0125530:CDS
AAACATGAAATTCTTGAGAT+TGG	0.333905	1_3:+53992543	Msa0125530:five_prime_UTR
AAACCAGCCGTAAATAAAAT+CGG	0.474194	1_3:-53991998	None:intergenic
AAAGAAGAGCTTCAGAATAA+TGG	0.273819	1_3:+53989727	Msa0125530:five_prime_UTR
AAAGGTCGCGGCTGTGAATG+GGG	0.574527	1_3:+53988188	Msa0125530:five_prime_UTR
AACAAGTGAGTGAACAAAAG+TGG	0.499916	1_3:-53985338	None:intergenic
AACCACATTGACTCCACCTT+TGG	0.409238	1_3:-53985571	None:intergenic
AACTCACGTTAAAGAACCAG+TGG	0.662119	1_3:-53990935	None:intergenic
AACTCTCAAAACACTCTTGG+GGG	0.623792	1_3:-53991259	None:intergenic
AAGAAGAGCTTCAGAATAAT+GGG	0.387322	1_3:+53989728	Msa0125530:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTATGAGAAAGAAGATTACT+TGG	+	chr1_3:53985596-53985615	Msa0125530:five_prime_UTR	30.0%
TCAATTGCTCATAATTCATG+AGG	+	chr1_3:53985732-53985751	Msa0125530:intron	30.0%
ACAACTTCACATTGTATCAT+AGG	-	chr1_3:53985985-53986004	None:intergenic	30.0%
ATTACAAAGGGCTTAATAAC+AGG	-	chr1_3:53986030-53986049	None:intergenic	30.0%
TAAGGTAAATAAGTGGTGTT+TGG	+	chr1_3:53986785-53986804	Msa0125530:intron	30.0%
AAGGTAAATAAGTGGTGTTT+GGG	+	chr1_3:53986786-53986805	Msa0125530:intron	30.0%
TGTCAACTGAATTATGTTCA+CGG	+	chr1_3:53986849-53986868	Msa0125530:intron	30.0%
GAAAAGAACAGGAAGATATA+TGG	+	chr1_3:53987060-53987079	Msa0125530:intron	30.0%
TATCTTTAATCCTTGAAAGG+AGG	-	chr1_3:53987239-53987258	None:intergenic	30.0%
GTTTGACCAATAATTGTTTC+AGG	+	chr1_3:53987655-53987674	Msa0125530:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	53985300	53994290	53985300	ID=Msa0125530;Name=Msa0125530
chr1_3	mRNA	53985300	53994290	53985300	ID=Msa0125530-mRNA-1;Parent=Msa0125530;Name=Msa0125530-mRNA-1;_AED=0.20;_eAED=0.20;_QI=2238\|0.95\|1\|1\|0.63\|0.73\|23\|246\|164
chr1_3	exon	53985300	53985686	53985300	ID=Msa0125530-mRNA-1:exon:16628;Parent=Msa0125530-mRNA-1
chr1_3	exon	53988101	53988318	53988101	ID=Msa0125530-mRNA-1:exon:16629;Parent=Msa0125530-mRNA-1
chr1_3	exon	53988918	53989033	53988918	ID=Msa0125530-mRNA-1:exon:16630;Parent=Msa0125530-mRNA-1
chr1_3	exon	53989135	53989236	53989135	ID=Msa0125530-mRNA-1:exon:16631;Parent=Msa0125530-mRNA-1
chr1_3	exon	53989336	53989395	53989336	ID=Msa0125530-mRNA-1:exon:16632;Parent=Msa0125530-mRNA-1
chr1_3	exon	53989724	53989802	53989724	ID=Msa0125530-mRNA-1:exon:16633;Parent=Msa0125530-mRNA-1
chr1_3	exon	53989902	53990048	53989902	ID=Msa0125530-mRNA-1:exon:16634;Parent=Msa0125530-mRNA-1
chr1_3	exon	53990128	53990251	53990128	ID=Msa0125530-mRNA-1:exon:16635;Parent=Msa0125530-mRNA-1
chr1_3	exon	53990339	53990519	53990339	ID=Msa0125530-mRNA-1:exon:16636;Parent=Msa0125530-mRNA-1
chr1_3	exon	53990636	53990664	53990636	ID=Msa0125530-mRNA-1:exon:16637;Parent=Msa0125530-mRNA-1
chr1_3	exon	53990803	53990950	53990803	ID=Msa0125530-mRNA-1:exon:16638;Parent=Msa0125530-mRNA-1
chr1_3	exon	53991203	53991305	53991203	ID=Msa0125530-mRNA-1:exon:16639;Parent=Msa0125530-mRNA-1
chr1_3	exon	53991666	53991761	53991666	ID=Msa0125530-mRNA-1:exon:16640;Parent=Msa0125530-mRNA-1
chr1_3	exon	53991850	53991902	53991850	ID=Msa0125530-mRNA-1:exon:16641;Parent=Msa0125530-mRNA-1
chr1_3	exon	53991985	53992108	53991985	ID=Msa0125530-mRNA-1:exon:16642;Parent=Msa0125530-mRNA-1
chr1_3	exon	53992208	53992269	53992208	ID=Msa0125530-mRNA-1:exon:16643;Parent=Msa0125530-mRNA-1
chr1_3	exon	53992387	53992446	53992387	ID=Msa0125530-mRNA-1:exon:16644;Parent=Msa0125530-mRNA-1
chr1_3	exon	53992528	53992690	53992528	ID=Msa0125530-mRNA-1:exon:16645;Parent=Msa0125530-mRNA-1
chr1_3	exon	53993043	53993136	53993043	ID=Msa0125530-mRNA-1:exon:16646;Parent=Msa0125530-mRNA-1
chr1_3	exon	53993313	53993449	53993313	ID=Msa0125530-mRNA-1:exon:16647;Parent=Msa0125530-mRNA-1
chr1_3	exon	53993604	53993684	53993604	ID=Msa0125530-mRNA-1:exon:16648;Parent=Msa0125530-mRNA-1
chr1_3	exon	53993785	53993890	53993785	ID=Msa0125530-mRNA-1:exon:16649;Parent=Msa0125530-mRNA-1
chr1_3	exon	53993982	53994290	53993982	ID=Msa0125530-mRNA-1:exon:16650;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53985300	53985686	53985300	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53988101	53988318	53988101	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53988918	53989033	53988918	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53989135	53989236	53989135	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53989336	53989395	53989336	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53989724	53989802	53989724	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53989902	53990048	53989902	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53990128	53990251	53990128	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53990339	53990519	53990339	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53990636	53990664	53990636	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53990803	53990950	53990803	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53991203	53991305	53991203	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53991666	53991761	53991666	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53991850	53991902	53991850	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53991985	53992108	53991985	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53992208	53992269	53992208	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53992387	53992446	53992387	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	five_prime_UTR	53992528	53992676	53992528	ID=Msa0125530-mRNA-1:five_prime_utr;Parent=Msa0125530-mRNA-1
chr1_3	CDS	53992677	53992690	53992677	ID=Msa0125530-mRNA-1:cds;Parent=Msa0125530-mRNA-1
chr1_3	CDS	53993043	53993136	53993043	ID=Msa0125530-mRNA-1:cds;Parent=Msa0125530-mRNA-1
chr1_3	CDS	53993313	53993449	53993313	ID=Msa0125530-mRNA-1:cds;Parent=Msa0125530-mRNA-1
chr1_3	CDS	53993604	53993684	53993604	ID=Msa0125530-mRNA-1:cds;Parent=Msa0125530-mRNA-1
chr1_3	CDS	53993785	53993890	53993785	ID=Msa0125530-mRNA-1:cds;Parent=Msa0125530-mRNA-1
chr1_3	CDS	53993982	53994044	53993982	ID=Msa0125530-mRNA-1:cds;Parent=Msa0125530-mRNA-1
chr1_3	three_prime_UTR	53994045	53994290	53994045	ID=Msa0125530-mRNA-1:three_prime_utr;Parent=Msa0125530-mRNA-1

Gene Sequence

>Msa0125530

ATGCTGGCCTTCAGTGAAATGATAGAAGCTGTTGGACATGAATACATGGAAGAATTCTTTGGTTGTTGTGAATCAGTAATGGCAAATGATGGACTTCTTGTTCTTCAGTTCATATCAATCCCGGATGAGCGTTATGATGAGTATAGGCGCAGTTCTGATTTTATAAAGGAATATATTTTTCCAGGAGGTTGCCTACCCTCTCTAAGTAGGATCACATCATCCATGGCATCCGCATCCAGACTATGCGTCGAGCACGTTGAAAACTTCGGAATTCATTACTATCAAACACTAAGATTGTGGAGAAAAAATTTCATGGAAAGGCAGAGTGAAATTTTGGCTCTAGGATTTAATGAAAAATTCATCAGAACATGGGAATACTATTTTGATTATTGTGGTGCTGGTTTTAAGTCACGCACACTTGGGAATTATCAGGTGGTTTTCTCACGCCCTGGGAACGTTACTGCATTCAGTGATCCATACAAAAGCTGGCCCTAA

Protein sequence

>Msa0125530

MLAFSEMIEAVGHEYMEEFFGCCESVMANDGLLVLQFISIPDERYDEYRRSSDFIKEYIFPGGCLPSLSRITSSMASASRLCVEHVENFGIHYYQTLRLWRKNFMERQSEILALGFNEKFIRTWEYYFDYCGAGFKSRTLGNYQVVFSRPGNVTAFSDPYKSWP*