AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1308820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1308820 MtrunA17_Chr8g0346021 99.094 552 5 0 1 552 1 552 0.0 1133
Msa1308820 MtrunA17_Chr6g0462191 34.375 384 182 15 112 486 89 411 1.96e-36 140
Msa1308820 MtrunA17_Chr1g0181581 29.167 384 214 9 112 486 101 435 7.44e-35 137
Msa1308820 MtrunA17_Chr7g0263731 31.738 397 208 13 107 486 88 438 1.54e-34 136
Msa1308820 MtrunA17_Chr4g0025441 46.040 202 88 7 126 323 95 279 1.80e-34 135
Msa1308820 MtrunA17_Chr1g0200161 39.378 193 99 4 126 314 140 318 2.23e-33 133
Msa1308820 MtrunA17_Chr4g0045641 28.598 528 278 19 1 485 1 472 6.55e-33 132
Msa1308820 MtrunA17_Chr3g0126901 35.754 358 165 10 1 333 1 318 1.28e-31 127
Msa1308820 MtrunA17_Chr1g0146931 44.444 207 96 6 116 305 111 315 8.35e-29 119
Msa1308820 MtrunA17_Chr3g0135851 41.451 193 87 4 124 305 88 265 5.14e-27 114
Msa1308820 MtrunA17_Chr6g0472551 30.685 365 168 11 129 477 119 414 8.33e-27 113
Msa1308820 MtrunA17_Chr4g0020671 30.892 314 146 13 1 300 1 257 7.47e-26 110
Msa1308820 MtrunA17_Chr4g0057321 28.764 445 223 12 30 467 15 372 7.48e-25 107
Msa1308820 MtrunA17_Chr1g0204901 48.148 162 71 4 45 197 48 205 6.60e-24 104
Msa1308820 MtrunA17_Chr8g0340761 45.026 191 82 4 44 226 36 211 1.95e-22 99.8
Msa1308820 MtrunA17_Chr1g0203711 30.878 353 156 11 126 466 114 390 3.20e-22 99.8
Msa1308820 MtrunA17_Chr2g0283321 33.880 183 101 5 126 305 62 227 5.71e-22 96.7
Msa1308820 MtrunA17_Chr8g0342551 40.000 190 88 4 125 305 88 260 1.24e-20 94.7
Msa1308820 MtrunA17_Chr8g0389781 75.000 60 15 0 126 185 125 184 2.84e-20 93.6
Msa1308820 MtrunA17_Chr1g0166431 43.165 139 59 3 45 179 47 169 6.83e-20 92.4
Msa1308820 MtrunA17_Chr7g0273861 32.240 183 105 4 118 300 116 279 5.81e-19 89.4
Msa1308820 MtrunA17_Chr7g0273951 40.411 146 61 3 47 191 50 170 2.66e-18 87.0
Msa1308820 MtrunA17_Chr8g0371131 62.295 61 23 0 125 185 69 129 2.10e-17 79.3
Msa1308820 MtrunA17_Chr2g0330921 61.429 70 27 0 124 193 94 163 5.84e-17 82.8
Msa1308820 MtrunA17_Chr4g0060381 61.905 63 24 0 129 191 102 164 6.48e-17 84.0
Msa1308820 MtrunA17_Chr2g0316401 29.949 197 112 8 127 316 74 251 2.33e-16 80.1
Msa1308820 MtrunA17_Chr2g0330051 37.500 200 81 5 125 303 76 252 3.65e-16 80.9
Msa1308820 MtrunA17_Chr1g0156121 59.649 57 23 0 129 185 126 182 8.12e-14 74.7
Msa1308820 MtrunA17_Chr5g0416161 34.615 130 69 4 60 183 56 175 1.18e-11 67.4
Msa1308820 MtrunA17_Chr7g0215371 54.545 55 25 0 129 183 21 75 2.62e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1308820 AT1G01110.2 57.886 577 168 25 1 552 1 527 0.0 522
Msa1308820 AT4G00820.1 58.007 562 180 18 9 552 11 534 1.24e-179 517
Msa1308820 AT1G01110.1 59.649 399 110 18 170 552 1 364 1.01e-136 401
Msa1308820 AT4G10640.1 36.219 566 204 19 1 552 1 423 5.24e-89 281
Msa1308820 AT3G49380.1 36.735 245 116 5 1 239 1 212 4.19e-36 138
Msa1308820 AT3G49260.1 34.853 373 176 14 1 331 1 348 1.02e-35 139
Msa1308820 AT3G49260.4 34.853 373 176 14 1 331 1 348 1.02e-35 139
Msa1308820 AT3G49260.2 34.853 373 176 14 1 331 1 348 1.02e-35 139
Msa1308820 AT2G26180.1 42.601 223 96 6 119 330 76 277 2.45e-35 137
Msa1308820 AT3G49260.3 35.040 371 179 14 1 331 1 349 6.36e-35 137
Msa1308820 AT5G03040.1 28.437 531 282 20 1 503 1 461 8.26e-35 137
Msa1308820 AT5G03040.3 28.437 531 282 20 1 503 1 461 8.26e-35 137
Msa1308820 AT5G03040.2 28.437 531 282 20 1 503 1 461 8.26e-35 137
Msa1308820 AT3G22190.3 43.005 193 80 5 123 305 84 256 4.26e-31 125
Msa1308820 AT3G22190.2 43.005 193 80 5 123 305 84 256 4.26e-31 125
Msa1308820 AT3G22190.4 43.005 193 80 5 123 305 84 256 4.26e-31 125
Msa1308820 AT3G22190.1 43.005 193 80 5 123 305 84 256 4.26e-31 125
Msa1308820 AT3G59690.2 26.629 537 257 18 1 485 1 452 1.56e-29 122
Msa1308820 AT3G59690.1 26.629 537 257 18 1 485 1 452 1.56e-29 122
Msa1308820 AT1G72670.1 37.981 208 103 6 107 305 73 263 1.58e-29 121
Msa1308820 AT3G52290.1 29.842 506 229 22 1 484 1 402 4.42e-29 120
Msa1308820 AT2G43680.1 26.859 417 224 14 126 539 322 660 7.14e-26 112
Msa1308820 AT2G43680.4 26.859 417 224 14 126 539 321 659 7.19e-26 112
Msa1308820 AT2G43680.2 26.859 417 224 14 126 539 321 659 7.19e-26 112
Msa1308820 AT2G43680.3 26.859 417 224 14 126 539 321 659 7.19e-26 112
Msa1308820 AT2G43680.5 26.859 417 224 14 126 539 321 659 7.19e-26 112
Msa1308820 AT5G13460.1 30.576 278 148 7 38 300 18 265 1.64e-23 103
Msa1308820 AT4G14750.1 69.697 66 20 0 126 191 104 169 3.53e-22 99.4
Msa1308820 AT1G17480.1 37.427 171 80 4 100 270 68 211 6.38e-22 98.2
Msa1308820 AT4G14750.2 69.697 66 20 0 126 191 163 228 7.82e-22 99.0
Msa1308820 AT4G14750.3 69.697 66 20 0 126 191 163 228 9.10e-22 99.0
Msa1308820 AT3G09710.2 31.220 205 117 7 111 303 95 287 1.04e-21 98.6
Msa1308820 AT3G09710.1 31.156 199 111 7 111 303 95 273 1.04e-21 98.6
Msa1308820 AT2G26410.2 31.188 202 115 6 123 318 144 327 2.88e-21 97.8
Msa1308820 AT2G26410.1 31.188 202 115 6 123 318 144 327 2.88e-21 97.8
Msa1308820 AT3G16490.1 67.143 70 23 0 121 190 101 170 1.04e-19 91.7
Msa1308820 AT4G23060.1 28.532 361 164 11 114 467 152 425 9.22e-19 89.7
Msa1308820 AT4G29150.1 45.082 122 56 2 122 241 126 238 1.29e-18 88.6
Msa1308820 AT5G07240.1 69.355 62 19 0 124 185 103 164 3.60e-18 87.4
Msa1308820 AT5G07240.2 69.355 62 19 0 124 185 149 210 4.77e-18 87.4
Msa1308820 AT5G62070.1 72.414 58 16 0 124 181 114 171 4.37e-17 84.0
Msa1308820 AT1G19870.1 37.037 135 68 2 71 205 173 290 2.02e-16 83.2
Msa1308820 AT1G18840.1 50.704 71 35 0 121 191 103 173 2.08e-16 82.8
Msa1308820 AT1G18840.3 50.704 71 35 0 121 191 103 173 2.08e-16 82.8
Msa1308820 AT1G18840.2 50.704 71 35 0 121 191 103 173 2.08e-16 82.8
Msa1308820 AT1G51960.1 61.538 65 25 0 126 190 99 163 1.10e-15 79.3
Msa1308820 AT2G33990.1 26.601 203 129 4 126 326 46 230 1.98e-15 77.0
Msa1308820 AT1G19870.2 54.667 75 31 1 131 205 36 107 2.11e-15 79.7
Msa1308820 AT3G51380.1 49.412 85 42 1 108 192 19 102 1.82e-14 70.1
Msa1308820 AT3G15050.1 26.630 184 115 4 129 308 52 219 1.87e-14 73.9
Msa1308820 AT1G74690.1 53.846 65 30 0 127 191 113 177 3.72e-14 75.9
Msa1308820 AT1G14380.1 49.231 65 33 0 129 193 96 160 1.86e-13 73.6
Msa1308820 AT1G14380.3 49.231 65 33 0 129 193 96 160 1.86e-13 73.6
Msa1308820 AT1G14380.4 49.231 65 33 0 129 193 96 160 1.86e-13 73.6
Msa1308820 AT1G14380.6 49.231 65 33 0 129 193 96 160 2.21e-13 73.2
Msa1308820 AT1G14380.5 49.231 65 33 0 129 193 96 160 2.21e-13 73.2
Msa1308820 AT1G14380.2 49.231 65 33 0 129 193 96 160 2.21e-13 73.2
Msa1308820 AT1G14380.7 49.231 65 33 0 129 193 96 160 2.41e-13 73.2

Find 175 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATAACATGTAATATTT+TGG 0.059916 8_4:+5176468 Msa1308820:three_prime_UTR
TTTACATAATAATAAAATTT+TGG 0.148736 8_4:+5172434 Msa1308820:five_prime_UTR
GTGACAAGACAGTCAATTTA+AGG 0.178332 8_4:-5176448 None:intergenic
GAAATTATGGACATAGTTTA+AGG 0.213922 8_4:+5175922 Msa1308820:CDS
TTGTTCCACCCCTACTATTT+TGG 0.218545 8_4:-5175686 None:intergenic
ATCCATCTATCTAGCCATTT+TGG 0.221443 8_4:-5175297 None:intergenic
ATCTAATTGTGCAAAGCTTA+TGG 0.234585 8_4:-5172408 None:intergenic
TGTGGATTATATTGATAATT+TGG 0.238180 8_4:-5175444 None:intergenic
ACCATTCACTTAATTTATTT+AGG 0.281624 8_4:+5176174 Msa1308820:three_prime_UTR
AGTACAACAAGAAGAGTAAT+TGG 0.281665 8_4:-5176003 None:intergenic
AAGAAACACTTGCGGATCTT+TGG 0.293669 8_4:-5172839 None:intergenic
CAGTGACACTGCTAGTGTTT+GGG 0.297942 8_4:+5174193 Msa1308820:CDS
CCAACTCCTCCTACATTATA+AGG 0.303150 8_4:-5175894 None:intergenic
GGAGTTGGTTATGGAAATTA+TGG 0.312677 8_4:+5175909 Msa1308820:CDS
TAGTGAGAGGTCACAATGTT+AGG 0.313052 8_4:+5174057 Msa1308820:CDS
AAGAGCACTTCGTGCGCTTA+AGG 0.319760 8_4:+5174013 Msa1308820:CDS
TGATTAATGATTCAATATAA+TGG 0.323692 8_4:-5172527 None:intergenic
TACAAAGCCATGGGAGAATA+GGG 0.328126 8_4:+5175323 Msa1308820:CDS
CTCCATAGATCACACCAAAA+TGG 0.330093 8_4:+5175501 Msa1308820:CDS
GGTTGTGAACGGATCCATTT+TGG 0.332251 8_4:-5175515 None:intergenic
GAGAACCATGTATTGTGTTT+TGG 0.353766 8_4:+5176325 Msa1308820:three_prime_UTR
TGCAATGACTGCGGAGTATT+CGG 0.357390 8_4:+5176121 Msa1308820:three_prime_UTR
GAAGTAGTATTGCAGATGAT+TGG 0.362742 8_4:+5174479 Msa1308820:CDS
CTCATCTCAACCACCGTCTA+AGG 0.364361 8_4:-5176067 None:intergenic
TACACATTCAACAATGTTAC+AGG 0.365782 8_4:-5172789 None:intergenic
GGTTACAACTATGACACATT+AGG 0.366301 8_4:+5176142 Msa1308820:three_prime_UTR
ACATCATGCTTCAACTTTGT+TGG 0.375678 8_4:-5173365 None:intergenic
CTACAAAGCCATGGGAGAAT+AGG 0.377195 8_4:+5175322 Msa1308820:CDS
TTTGCTCCTAGATATGAATA+AGG 0.377417 8_4:-5175405 None:intergenic
TTGCAGTGTTGAAGACATAA+AGG 0.379777 8_4:-5172923 None:intergenic
TCAGTGACACTGCTAGTGTT+TGG 0.380570 8_4:+5174192 Msa1308820:CDS
TCTCAACCTTATTCATATCT+AGG 0.385222 8_4:+5175399 Msa1308820:CDS
GGAACTTGTACCAAAATAGT+AGG 0.389102 8_4:+5175676 Msa1308820:CDS
GTGCTACAAGTGTGGCTTCA+AGG 0.412635 8_4:+5173393 Msa1308820:CDS
TAAATGCTGGCCGTATGTTC+TGG 0.412801 8_4:+5176378 Msa1308820:three_prime_UTR
AATGTAGGAGGAGTTGGTTA+TGG 0.413219 8_4:+5175900 Msa1308820:CDS
TCGGTTGCTTGCATGTAATT+AGG 0.413740 8_4:-5175759 None:intergenic
GGAGTCGATGGCCTTTGCCT+TGG 0.415194 8_4:-5175816 None:intergenic
GAGGTTTCACCTTCGTCAAC+CGG 0.431189 8_4:+5176041 Msa1308820:CDS
ATGTTCTGGCCTACCTCACT+TGG 0.431460 8_4:+5176392 Msa1308820:three_prime_UTR
AGTGAGTGAGTGAGAGGAGG+AGG 0.431837 8_4:-5172479 None:intergenic
CATACGGCCAGCATTTATCT+TGG 0.433048 8_4:-5176372 None:intergenic
CTTGACGATCGGACTTGAAT+AGG 0.433293 8_4:-5175573 None:intergenic
AATATTACATTGCAAACAAA+AGG 0.434379 8_4:-5176508 None:intergenic
TGTCTTTGTGGTTGGTGATG+TGG 0.435429 8_4:-5175462 None:intergenic
CCTCTCAAACACCAAGCTTA+AGG 0.437895 8_4:+5175634 Msa1308820:CDS
GACCAAAATGGCTAGATAGA+TGG 0.442192 8_4:+5175295 Msa1308820:CDS
TTCACTGTTTCATGATTCAC+TGG 0.442484 8_4:-5173323 None:intergenic
CCTCCTACATTATAAGGATC+AGG 0.445401 8_4:-5175888 None:intergenic
GGAAGAAAGGTAGTAATTCA+TGG 0.448229 8_4:+5173028 Msa1308820:CDS
GAGTGAGTGAGAGGAGGAGG+GGG 0.450034 8_4:-5172476 None:intergenic
AAGTAGTATTGCAGATGATT+GGG 0.451725 8_4:+5174480 Msa1308820:CDS
GGTACAAGTTCCCATTGTAA+TGG 0.452470 8_4:-5175665 None:intergenic
ATTTCACAGATTTGGAGAAA+TGG 0.454413 8_4:+5175231 Msa1308820:intron
TTCTTTCCTCTGTTGCAACA+TGG 0.456273 8_4:-5174536 None:intergenic
CATAAAGGTTCAACTTGGTT+GGG 0.456430 8_4:-5172908 None:intergenic
AAACCTGTTGTGAAAATGCT+TGG 0.456509 8_4:-5174588 None:intergenic
CCTTATAATGTAGGAGGAGT+TGG 0.456681 8_4:+5175894 Msa1308820:CDS
TTCATTTATTTGCACAAATG+AGG 0.457015 8_4:+5172758 Msa1308820:five_prime_UTR
ACATAAAGGTTCAACTTGGT+TGG 0.460178 8_4:-5172909 None:intergenic
GGTCTAATTACCATTACAAT+GGG 0.460991 8_4:+5175655 Msa1308820:CDS
ACATTCACACTCTTGAAACT+TGG 0.462543 8_4:-5175948 None:intergenic
CTGCTAGTGTTTGGGATTCG+CGG 0.462678 8_4:+5174201 Msa1308820:CDS
AGAGCACTTCGTGCGCTTAA+GGG 0.465270 8_4:+5174014 Msa1308820:CDS
GTGAAGTAGACCTTATCCTT+TGG 0.466662 8_4:-5174145 None:intergenic
TCAAGAAGAAGATGAAGAAA+AGG 0.473802 8_4:+5173131 Msa1308820:CDS
ACACACAGTTGAAGAAGTTA+AGG 0.475450 8_4:+5174513 Msa1308820:CDS
AATGGTAGGACATCATCAAT+TGG 0.477140 8_4:+5175249 Msa1308820:CDS
CGCAGAGTGCGCCAAGGCAA+AGG 0.484032 8_4:+5175805 Msa1308820:CDS
GCCTAAATAAATTAAGTGAA+TGG 0.484719 8_4:-5176175 None:intergenic
TGTGGCTTCAAGGACAGATC+AGG 0.489395 8_4:+5173403 Msa1308820:CDS
TGAGTGAGTGAGAGGAGGAG+GGG 0.489708 8_4:-5172477 None:intergenic
GGAAAGAAGCAGCAATGAAG+AGG 0.490592 8_4:+5174551 Msa1308820:CDS
ACATTATAAGGATCAGGAGC+AGG 0.491564 8_4:-5175882 None:intergenic
AATGTAGCAGCATCACATTA+TGG 0.491586 8_4:+5175966 Msa1308820:CDS
TTCATAACATTAAAAGCCTA+AGG 0.491869 8_4:+5172337 Msa1308820:five_prime_UTR
AGTCTGCCAAGGCTCGAATT+CGG 0.493661 8_4:+5175781 Msa1308820:CDS
CGTCAACCGGTGACCTTAGA+CGG 0.494779 8_4:+5176054 Msa1308820:CDS
TAAAGTCTTTAAAGAAGAGA+AGG 0.495381 8_4:-5172882 None:intergenic
GAACTTGTACCAAAATAGTA+GGG 0.496333 8_4:+5175677 Msa1308820:CDS
GTGAGTGAGTGAGAGGAGGA+GGG 0.498570 8_4:-5172478 None:intergenic
GATCCGGATCAGTAAAGAAA+AGG 0.499138 8_4:+5175850 Msa1308820:CDS
ACATGCTCTTGCTGTGGCTA+TGG 0.501053 8_4:+5173433 Msa1308820:CDS
GCCCTTCCCCTATTCTCCCA+TGG 0.501799 8_4:-5175330 None:intergenic
TGTTCTGGCCTACCTCACTT+GGG 0.503064 8_4:+5176393 Msa1308820:three_prime_UTR
GATCCGGATCTATCCCTCTC+TGG 0.503639 8_4:-5175837 None:intergenic
CTTAATAGAGTCTCTTTGAT+CGG 0.504047 8_4:-5175358 None:intergenic
CTAAGAGTTCAAATCATGCT+AGG 0.504978 8_4:+5173513 Msa1308820:CDS
TAAGGACATACTTGTTGTGT+AGG 0.509479 8_4:-5176430 None:intergenic
AACTTGAAGAGAGACCAAAA+TGG 0.518980 8_4:+5175283 Msa1308820:CDS
TGCTGGAGACGGAGTAGTGA+TGG 0.522782 8_4:-5175541 None:intergenic
GCTCCTGATCCTTATAATGT+AGG 0.524729 8_4:+5175885 Msa1308820:CDS
CTATCCCTCTCTGGAGTCGA+TGG 0.525156 8_4:-5175828 None:intergenic
CCTTAAGCTTGGTGTTTGAG+AGG 0.526162 8_4:-5175634 None:intergenic
GTGAGGTAGGCCAGAACATA+CGG 0.527112 8_4:-5176388 None:intergenic
AACCTGTTGTGAAAATGCTT+GGG 0.527631 8_4:-5174587 None:intergenic
AGGTCTAATTACCATTACAA+TGG 0.529005 8_4:+5175654 Msa1308820:CDS
GAGTTCTTGACCAAAGGATA+AGG 0.529710 8_4:+5174135 Msa1308820:CDS
ATCTTCAAGACATTTCAGAT+AGG 0.531548 8_4:+5174225 Msa1308820:CDS
TCACAAAGAAACAAACAGTA+AGG 0.537387 8_4:+5172656 Msa1308820:five_prime_UTR
ATATAATGGTAGTCAGAGAC+AGG 0.538519 8_4:-5172513 None:intergenic
AATTCGAGCCTTGGCAGACT+CGG 0.539728 8_4:-5175778 None:intergenic
AGTGTCAGAAGGTATACCTT+AGG 0.542037 8_4:-5172353 None:intergenic
AGCTACTGCTCAAGCTGCCG+TGG 0.542160 8_4:+5173475 Msa1308820:CDS
GAGCACTTCGTGCGCTTAAG+GGG 0.545080 8_4:+5174015 Msa1308820:CDS
AGCAGCATCACATTATGGAT+TGG 0.546365 8_4:+5175971 Msa1308820:CDS
GAAGCAATGGAATGTCTTTG+TGG 0.547093 8_4:-5175474 None:intergenic
GAAAGAAGCAGCAATGAAGA+GGG 0.550092 8_4:+5174552 Msa1308820:CDS
TCTTGTTGTACTGAGAGTCT+CGG 0.552055 8_4:+5176014 Msa1308820:CDS
AGCTTGGTGTTTGAGAGGTG+TGG 0.552600 8_4:-5175629 None:intergenic
TTAACTTCTTCAACTGTGTG+TGG 0.553478 8_4:-5174511 None:intergenic
GCAAGTACTATGTTGTTGCT+AGG 0.553496 8_4:+5176297 Msa1308820:three_prime_UTR
CACAACGAGGGCTTGACGAT+CGG 0.555443 8_4:-5175584 None:intergenic
AGTCAACCTTGCAACTTCCA+CGG 0.555760 8_4:-5173492 None:intergenic
TACTGAGAGTCTCGGTGGCG+AGG 0.556933 8_4:+5176022 Msa1308820:CDS
CAATGGAATGTCTTTGTGGT+TGG 0.558998 8_4:-5175470 None:intergenic
ACTCCAGAGAGGGATAGATC+CGG 0.559569 8_4:+5175834 Msa1308820:CDS
AGTAGGGGTGGAACAAGTAA+TGG 0.561747 8_4:+5175693 Msa1308820:CDS
CTGCGACCGAATTCGAGCCT+TGG 0.565424 8_4:-5175787 None:intergenic
CACAGATTTGGAGAAATGGT+AGG 0.567014 8_4:+5175235 Msa1308820:intron
TCAACTACACTCAGTTTCAA+CGG 0.568119 8_4:+5176095 Msa1308820:three_prime_UTR
TAGGATGCCAAGATAAATGC+TGG 0.570025 8_4:+5176365 Msa1308820:three_prime_UTR
TAAAACCAAAACACAATACA+TGG 0.570954 8_4:-5176330 None:intergenic
AGATTATGTTGAGTACTACA+AGG 0.572264 8_4:-5172571 None:intergenic
ATTGATTGAAACAAAAGCAA+AGG 0.575368 8_4:-5176534 None:intergenic
GAGACGGAGTAGTGATGGAT+TGG 0.577607 8_4:-5175536 None:intergenic
TTTGTGAGTGAGTGAGTGAG+AGG 0.580321 8_4:-5172485 None:intergenic
TGGTAATTAGACCTTAAGCT+TGG 0.583935 8_4:-5175645 None:intergenic
CCTGATCCTTATAATGTAGG+AGG 0.585582 8_4:+5175888 Msa1308820:CDS
GATGGATGGCTACAAAGCCA+TGG 0.586531 8_4:+5175313 Msa1308820:CDS
TGTGGCTATGGCTACAGCTG+AGG 0.587150 8_4:+5173445 Msa1308820:CDS
CAACCGGTGACCTTAGACGG+TGG 0.593652 8_4:+5176057 Msa1308820:CDS
TAAGAGTTCAAATCATGCTA+GGG 0.594458 8_4:+5173514 Msa1308820:CDS
TTGTGAAAATGCTTGGGACA+AGG 0.594968 8_4:-5174581 None:intergenic
AAGTTGCAAGCTTTAGTGAG+AGG 0.597297 8_4:+5174044 Msa1308820:CDS
GAAGACATAAAGGTTCAACT+TGG 0.599432 8_4:-5172913 None:intergenic
GTGAGTGAGTGAGTGAGAGG+AGG 0.601432 8_4:-5172482 None:intergenic
TTGTACCAAAATAGTAGGGG+TGG 0.602042 8_4:+5175681 Msa1308820:CDS
ATAAGGTCTACTTCACATGA+AGG 0.602228 8_4:+5174152 Msa1308820:CDS
AAGCAAGAGTTCTTGACCAA+AGG 0.602268 8_4:+5174129 Msa1308820:CDS
AAGCTGCCGTGGAAGTTGCA+AGG 0.602390 8_4:+5173486 Msa1308820:CDS
TTAAGGCCATGTTGCAACAG+AGG 0.604888 8_4:+5174530 Msa1308820:CDS
TTGGTGTTTGAGAGGTGTGG+TGG 0.605794 8_4:-5175626 None:intergenic
CAACCACCGTCTAAGGTCAC+CGG 0.606102 8_4:-5176060 None:intergenic
AAAGGTTCAACTTGGTTGGG+TGG 0.612492 8_4:-5172905 None:intergenic
TCTATGGAGTGGTGAAGCAA+TGG 0.613224 8_4:-5175487 None:intergenic
ATGGATGGCTACAAAGCCAT+GGG 0.613295 8_4:+5175314 Msa1308820:CDS
GTTCTGGCCTACCTCACTTG+GGG 0.613716 8_4:+5176394 Msa1308820:three_prime_UTR
GCAAGCAACCGAGTCTGCCA+AGG 0.614885 8_4:+5175770 Msa1308820:CDS
AGCCATGGGAGAATAGGGGA+AGG 0.621685 8_4:+5175328 Msa1308820:CDS
CATTATAAGGATCAGGAGCA+GGG 0.621699 8_4:-5175881 None:intergenic
AGCAAGCAAAGATGACACTT+AGG 0.631862 8_4:+5174081 Msa1308820:CDS
ACAGCAGTGGGAGTGTCAGA+AGG 0.634678 8_4:-5172364 None:intergenic
AACACTAGCAGTGTCACTGA+AGG 0.637230 8_4:-5174189 None:intergenic
AAAATGGCTAGATAGATGGA+TGG 0.637956 8_4:+5175299 Msa1308820:CDS
TTCGGTCGCAGAGTGCGCCA+AGG 0.638278 8_4:+5175799 Msa1308820:CDS
AAGAGACTCTATTAAGACTG+TGG 0.640275 8_4:+5175365 Msa1308820:CDS
AAGGCCATCGACTCCAGAGA+GGG 0.644870 8_4:+5175824 Msa1308820:CDS
GCCATGGGAGAATAGGGGAA+GGG 0.647158 8_4:+5175329 Msa1308820:CDS
TTCAACACCCCAAGTGAGGT+AGG 0.648136 8_4:-5176401 None:intergenic
GCATGAAGGTGGACAGCAGT+GGG 0.649814 8_4:-5172376 None:intergenic
CTAAGGTCACCGGTTGACGA+AGG 0.652413 8_4:-5176050 None:intergenic
ACAAAGCCATGGGAGAATAG+GGG 0.653021 8_4:+5175324 Msa1308820:CDS
CAACGGCTCTGCAATGACTG+CGG 0.660325 8_4:+5176112 Msa1308820:three_prime_UTR
AGTGATGGATTGGTTGTGAA+CGG 0.660603 8_4:-5175526 None:intergenic
ATTGTTCAACACCCCAAGTG+AGG 0.663712 8_4:-5176405 None:intergenic
CTATCTTCTCGAACACAACG+AGG 0.664998 8_4:-5175597 None:intergenic
TATCTTCTCGAACACAACGA+GGG 0.667052 8_4:-5175596 None:intergenic
TGTTGTTAGTGCTACAAGTG+TGG 0.667700 8_4:+5173385 Msa1308820:CDS
AACTTGTACCAAAATAGTAG+GGG 0.675624 8_4:+5175678 Msa1308820:CDS
TGAGAAACATGCTCTTGCTG+TGG 0.692861 8_4:+5173427 Msa1308820:CDS
TGTTGTACTGAGAGTCTCGG+TGG 0.693541 8_4:+5176017 Msa1308820:CDS
GGCATGAAGGTGGACAGCAG+TGG 0.698214 8_4:-5172377 None:intergenic
AAAGGCCATCGACTCCAGAG+AGG 0.711647 8_4:+5175823 Msa1308820:CDS
AAAGCTAAAAGAAACACTTG+CGG 0.714126 8_4:-5172847 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGAACTTTTTAATTATTT+TGG + chr8_4:5174393-5174412 Msa1308820:intron 10.0%
!!! ATAATTGATTTTGAATTTTT+TGG + chr8_4:5174930-5174949 Msa1308820:intron 10.0%
!! TGATTAATGATTCAATATAA+TGG - chr8_4:5172530-5172549 None:intergenic 15.0%
!!! AATATTTTATAGTAGTACAT+TGG + chr8_4:5172613-5172632 Msa1308820:five_prime_UTR 15.0%
!! ATTAGAATGTAAAAACAAAA+GGG - chr8_4:5173207-5173226 None:intergenic 15.0%
!! AATTAGAATGTAAAAACAAA+AGG - chr8_4:5173208-5173227 None:intergenic 15.0%
!!! ATATTTTGGAATTTCAATTA+TGG + chr8_4:5176482-5176501 Msa1308820:three_prime_UTR 15.0%
!!! AAATGTTGTTAAGTTTTACA+TGG + chr8_4:5173005-5173024 Msa1308820:exon 20.0%
!!! AATGTTGTTAAGTTTTACAT+GGG + chr8_4:5173006-5173025 Msa1308820:exon 20.0%
!!! TGTTTTTACATTCTAATTCA+TGG + chr8_4:5173210-5173229 Msa1308820:intron 20.0%
!! GGTTAGAAAATTTAATACAT+GGG - chr8_4:5173913-5173932 None:intergenic 20.0%
!! AGGTTAGAAAATTTAATACA+TGG - chr8_4:5173914-5173933 None:intergenic 20.0%
!! AAGAATTGATTGTGTAAAAT+TGG - chr8_4:5175111-5175130 None:intergenic 20.0%
!!! TACACAATCAATTCTTTTTT+TGG + chr8_4:5175115-5175134 Msa1308820:intron 20.0%
!!! TGTGGATTATATTGATAATT+TGG - chr8_4:5175447-5175466 None:intergenic 20.0%
!! ACCATTCACTTAATTTATTT+AGG + chr8_4:5176174-5176193 Msa1308820:three_prime_UTR 20.0%
!!! ATGTTAACTTCTTTTCTTTT+GGG - chr8_4:5176250-5176269 None:intergenic 20.0%
!!! AATGTTAACTTCTTTTCTTT+TGG - chr8_4:5176251-5176270 None:intergenic 20.0%
!! AACATTAAAAATCTGATGTT+AGG + chr8_4:5176265-5176284 Msa1308820:three_prime_UTR 20.0%
!!! CACAATAACATGTAATATTT+TGG + chr8_4:5176468-5176487 Msa1308820:three_prime_UTR 20.0%
!! AATATTACATTGCAAACAAA+AGG - chr8_4:5176511-5176530 None:intergenic 20.0%
! TTCATAACATTAAAAGCCTA+AGG + chr8_4:5172337-5172356 Msa1308820:five_prime_UTR 25.0%
! TTCATTTATTTGCACAAATG+AGG + chr8_4:5172758-5172777 Msa1308820:five_prime_UTR 25.0%
!! TAAAGTCTTTAAAGAAGAGA+AGG - chr8_4:5172885-5172904 None:intergenic 25.0%
!! ATGTTGTTAAGTTTTACATG+GGG + chr8_4:5173007-5173026 Msa1308820:exon 25.0%
!!! CTTTTTTCACTATCTTTTGT+TGG - chr8_4:5173084-5173103 None:intergenic 25.0%
! AAAGATAGTGAAAAAAGAAG+TGG + chr8_4:5173087-5173106 Msa1308820:CDS 25.0%
!!! AAAAAAGAAGGTGGATTTTT+AGG + chr8_4:5173297-5173316 Msa1308820:CDS 25.0%
!!! AACTTCTTTTAGAGGTTATT+TGG + chr8_4:5173562-5173581 Msa1308820:CDS 25.0%
! ATAACACAAACTTCTTATCA+AGG - chr8_4:5173934-5173953 None:intergenic 25.0%
!!! AAGTACCTTTTTTCTTTTCT+TGG + chr8_4:5173965-5173984 Msa1308820:intron 25.0%
! AAATTCGATTACAATGATGT+TGG - chr8_4:5174291-5174310 None:intergenic 25.0%
! TTAGAAGCAAAGATTATGTT+TGG + chr8_4:5174335-5174354 Msa1308820:intron 25.0%
!!! TATTTTGGTGTTCTGTTTAT+AGG + chr8_4:5174408-5174427 Msa1308820:intron 25.0%
! AGAAAATAAAGCGAAAAAGA+GGG - chr8_4:5174622-5174641 None:intergenic 25.0%
! AAGAAAATAAAGCGAAAAAG+AGG - chr8_4:5174623-5174642 None:intergenic 25.0%
!! AGCTCAAAATGTAGATTTTT+CGG - chr8_4:5174797-5174816 None:intergenic 25.0%
! TCTAACTTGAAACTAGAATT+TGG + chr8_4:5174974-5174993 Msa1308820:intron 25.0%
! ATACCAAAAATCAATTGTTG+AGG - chr8_4:5175008-5175027 None:intergenic 25.0%
!!! ATATTCAACTCACTTTTACA+TGG + chr8_4:5175036-5175055 Msa1308820:intron 25.0%
! AATAGCTAAGAAAATTGCTT+TGG - chr8_4:5175200-5175219 None:intergenic 25.0%
!! AAGCAATTTTCTTAGCTATT+TGG + chr8_4:5175200-5175219 Msa1308820:intron 25.0%
! CTTTAACAATTTCACAGATT+TGG + chr8_4:5175223-5175242 Msa1308820:intron 25.0%
! GAAATTATGGACATAGTTTA+AGG + chr8_4:5175922-5175941 Msa1308820:CDS 25.0%
! GCCTAAATAAATTAAGTGAA+TGG - chr8_4:5176178-5176197 None:intergenic 25.0%
!! TGTTAACTTCTTTTCTTTTG+GGG - chr8_4:5176249-5176268 None:intergenic 25.0%
! TAAAACCAAAACACAATACA+TGG - chr8_4:5176333-5176352 None:intergenic 25.0%
!!! GGTTTTATCATTTGTTTGTT+AGG + chr8_4:5176346-5176365 Msa1308820:three_prime_UTR 25.0%
! ATTGATTGAAACAAAAGCAA+AGG - chr8_4:5176537-5176556 None:intergenic 25.0%
ATCTAATTGTGCAAAGCTTA+TGG - chr8_4:5172411-5172430 None:intergenic 30.0%
AGATTATGTTGAGTACTACA+AGG - chr8_4:5172574-5172593 None:intergenic 30.0%
TCACAAAGAAACAAACAGTA+AGG + chr8_4:5172656-5172675 Msa1308820:five_prime_UTR 30.0%
TACACATTCAACAATGTTAC+AGG - chr8_4:5172792-5172811 None:intergenic 30.0%
AAAGCTAAAAGAAACACTTG+CGG - chr8_4:5172850-5172869 None:intergenic 30.0%
! TCAACACTGCAAAGTTTTTA+AGG + chr8_4:5172934-5172953 Msa1308820:five_prime_UTR 30.0%
TCAAGAAGAAGATGAAGAAA+AGG + chr8_4:5173131-5173150 Msa1308820:CDS 30.0%
TGTAGAAGAGAGAAAAAAGA+AGG + chr8_4:5173285-5173304 Msa1308820:intron 30.0%
TAAGAGTTCAAATCATGCTA+GGG + chr8_4:5173514-5173533 Msa1308820:CDS 30.0%
! GTGATTCAAACTTCTTTTAG+AGG + chr8_4:5173554-5173573 Msa1308820:CDS 30.0%
AACAGATCTAATTGCATGTT+TGG + chr8_4:5173596-5173615 Msa1308820:intron 30.0%
CTTTATGTCTGACAATATCA+TGG + chr8_4:5173689-5173708 Msa1308820:intron 30.0%
ACACACCAAGAAAAGAAAAA+AGG - chr8_4:5173973-5173992 None:intergenic 30.0%
ATCTTCAAGACATTTCAGAT+AGG + chr8_4:5174225-5174244 Msa1308820:CDS 30.0%
! AATCTTTGCTTCTAAAAGCA+TGG - chr8_4:5174330-5174349 None:intergenic 30.0%
!!! TTGGAAACAAAAGTGCTTTT+TGG + chr8_4:5174354-5174373 Msa1308820:intron 30.0%
!!! CTTTTTGGTTACAAAAGCAA+AGG + chr8_4:5174369-5174388 Msa1308820:intron 30.0%
! ATGTGTTTGAATCTTTTGTG+TGG + chr8_4:5174434-5174453 Msa1308820:intron 30.0%
! AATCTTTTGTGTGGTTGTTT+TGG + chr8_4:5174443-5174462 Msa1308820:intron 30.0%
AAGTAGTATTGCAGATGATT+GGG + chr8_4:5174480-5174499 Msa1308820:CDS 30.0%
! TTGTTCTAAGTCTGCATATT+TGG + chr8_4:5174641-5174660 Msa1308820:intron 30.0%
!! ATTTTTCGGCATAGTGATTT+TGG - chr8_4:5174783-5174802 None:intergenic 30.0%
!! TTGCCTCAACAATTGATTTT+TGG + chr8_4:5175002-5175021 Msa1308820:intron 30.0%
!! AAGAAAATTGCTTTGGTGTT+TGG - chr8_4:5175193-5175212 None:intergenic 30.0%
ATTTCACAGATTTGGAGAAA+TGG + chr8_4:5175231-5175250 Msa1308820:intron 30.0%
CTTAATAGAGTCTCTTTGAT+CGG - chr8_4:5175361-5175380 None:intergenic 30.0%
TCTCAACCTTATTCATATCT+AGG + chr8_4:5175399-5175418 Msa1308820:CDS 30.0%
TTTGCTCCTAGATATGAATA+AGG - chr8_4:5175408-5175427 None:intergenic 30.0%
AGGTCTAATTACCATTACAA+TGG + chr8_4:5175654-5175673 Msa1308820:CDS 30.0%
GGTCTAATTACCATTACAAT+GGG + chr8_4:5175655-5175674 Msa1308820:CDS 30.0%
GAACTTGTACCAAAATAGTA+GGG + chr8_4:5175677-5175696 Msa1308820:CDS 30.0%
AACTTGTACCAAAATAGTAG+GGG + chr8_4:5175678-5175697 Msa1308820:CDS 30.0%
AGTACAACAAGAAGAGTAAT+TGG - chr8_4:5176006-5176025 None:intergenic 30.0%
! TATGGATTTTTGTGGCATGA+AGG - chr8_4:5172393-5172412 None:intergenic 35.0%
! GCAAAGCTTATGGATTTTTG+TGG - chr8_4:5172401-5172420 None:intergenic 35.0%
ATATAATGGTAGTCAGAGAC+AGG - chr8_4:5172516-5172535 None:intergenic 35.0%
AGAAGAGAAGGAGAAAAACA+AGG - chr8_4:5172873-5172892 None:intergenic 35.0%
CATAAAGGTTCAACTTGGTT+GGG - chr8_4:5172911-5172930 None:intergenic 35.0%
ACATAAAGGTTCAACTTGGT+TGG - chr8_4:5172912-5172931 None:intergenic 35.0%
GAAGACATAAAGGTTCAACT+TGG - chr8_4:5172916-5172935 None:intergenic 35.0%
! TTGCAGTGTTGAAGACATAA+AGG - chr8_4:5172926-5172945 None:intergenic 35.0%
! AAGTTTTACATGGGGAAGAA+AGG + chr8_4:5173015-5173034 Msa1308820:exon 35.0%
GGAAGAAAGGTAGTAATTCA+TGG + chr8_4:5173028-5173047 Msa1308820:CDS 35.0%
AGAAGAGAGAAAAAAGAAGG+TGG + chr8_4:5173288-5173307 Msa1308820:intron 35.0%
TTCACTGTTTCATGATTCAC+TGG - chr8_4:5173326-5173345 None:intergenic 35.0%
ACATCATGCTTCAACTTTGT+TGG - chr8_4:5173368-5173387 None:intergenic 35.0%
CTAAGAGTTCAAATCATGCT+AGG + chr8_4:5173513-5173532 Msa1308820:CDS 35.0%
GAGCTTCAACAAAATTACAG+TGG - chr8_4:5173652-5173671 None:intergenic 35.0%
! ACCAATTGCCACAATGATTT+TGG + chr8_4:5173780-5173799 Msa1308820:intron 35.0%
TCCAAAATCATTGTGGCAAT+TGG - chr8_4:5173784-5173803 None:intergenic 35.0%
ATTTGGCAAAAACTACGCTT+TGG + chr8_4:5173843-5173862 Msa1308820:intron 35.0%
TTGACGAAATCACATTGACA+CGG - chr8_4:5173885-5173904 None:intergenic 35.0%
ATAAGGTCTACTTCACATGA+AGG + chr8_4:5174152-5174171 Msa1308820:CDS 35.0%
GAAGTAGTATTGCAGATGAT+TGG + chr8_4:5174479-5174498 Msa1308820:CDS 35.0%
TTAACTTCTTCAACTGTGTG+TGG - chr8_4:5174514-5174533 None:intergenic 35.0%
ACACACAGTTGAAGAAGTTA+AGG + chr8_4:5174513-5174532 Msa1308820:CDS 35.0%
AACCTGTTGTGAAAATGCTT+GGG - chr8_4:5174590-5174609 None:intergenic 35.0%
AAACCTGTTGTGAAAATGCT+TGG - chr8_4:5174591-5174610 None:intergenic 35.0%
TGCACTCATGAGTAAATGTT+CGG - chr8_4:5174865-5174884 None:intergenic 35.0%
AATGGTAGGACATCATCAAT+TGG + chr8_4:5175249-5175268 Msa1308820:CDS 35.0%
AACTTGAAGAGAGACCAAAA+TGG + chr8_4:5175283-5175302 Msa1308820:CDS 35.0%
! ATCCATCTATCTAGCCATTT+TGG - chr8_4:5175300-5175319 None:intergenic 35.0%
AAAATGGCTAGATAGATGGA+TGG + chr8_4:5175299-5175318 Msa1308820:CDS 35.0%
AAGAGACTCTATTAAGACTG+TGG + chr8_4:5175365-5175384 Msa1308820:CDS 35.0%
!! ATCCATTTTGGTGTGATCTA+TGG - chr8_4:5175506-5175525 None:intergenic 35.0%
! TGAATAGGTCTTGATTTTGC+TGG - chr8_4:5175561-5175580 None:intergenic 35.0%
TGGTAATTAGACCTTAAGCT+TGG - chr8_4:5175648-5175667 None:intergenic 35.0%
GGAACTTGTACCAAAATAGT+AGG + chr8_4:5175676-5175695 Msa1308820:CDS 35.0%
! AAGCCTTTTCTTTACTGATC+CGG - chr8_4:5175856-5175875 None:intergenic 35.0%
! GGAGTTGGTTATGGAAATTA+TGG + chr8_4:5175909-5175928 Msa1308820:CDS 35.0%
ACATTCACACTCTTGAAACT+TGG - chr8_4:5175951-5175970 None:intergenic 35.0%
AATGTAGCAGCATCACATTA+TGG + chr8_4:5175966-5175985 Msa1308820:CDS 35.0%
TCAACTACACTCAGTTTCAA+CGG + chr8_4:5176095-5176114 Msa1308820:three_prime_UTR 35.0%
GGTTACAACTATGACACATT+AGG + chr8_4:5176142-5176161 Msa1308820:three_prime_UTR 35.0%
!!! CTTTTCTTTTGGGGTGAAAA+GGG - chr8_4:5176240-5176259 None:intergenic 35.0%
!!! TCTTTTCTTTTGGGGTGAAA+AGG - chr8_4:5176241-5176260 None:intergenic 35.0%
! GAGAACCATGTATTGTGTTT+TGG + chr8_4:5176325-5176344 Msa1308820:three_prime_UTR 35.0%
!! TAAGGACATACTTGTTGTGT+AGG - chr8_4:5176433-5176452 None:intergenic 35.0%
GTGACAAGACAGTCAATTTA+AGG - chr8_4:5176451-5176470 None:intergenic 35.0%
! AGTGTCAGAAGGTATACCTT+AGG - chr8_4:5172356-5172375 None:intergenic 40.0%
AAGAAACACTTGCGGATCTT+TGG - chr8_4:5172842-5172861 None:intergenic 40.0%
TGTTGTTAGTGCTACAAGTG+TGG + chr8_4:5173385-5173404 Msa1308820:CDS 40.0%
CCACTGATCCAAAATCATTG+TGG - chr8_4:5173791-5173810 None:intergenic 40.0%
! CCACAATGATTTTGGATCAG+TGG + chr8_4:5173788-5173807 Msa1308820:intron 40.0%
! TTTTGGATCAGTGGTGAGTT+TGG + chr8_4:5173797-5173816 Msa1308820:intron 40.0%
CACAATGTTCAACCGTGATT+TGG + chr8_4:5173826-5173845 Msa1308820:intron 40.0%
! GCGTAGTTTTTGCCAAATCA+CGG - chr8_4:5173841-5173860 None:intergenic 40.0%
!! GGTGTGTTTTTGAACCTTGA+AGG + chr8_4:5173986-5174005 Msa1308820:intron 40.0%
! AAGTTGCAAGCTTTAGTGAG+AGG + chr8_4:5174044-5174063 Msa1308820:CDS 40.0%
TAGTGAGAGGTCACAATGTT+AGG + chr8_4:5174057-5174076 Msa1308820:CDS 40.0%
AGCAAGCAAAGATGACACTT+AGG + chr8_4:5174081-5174100 Msa1308820:CDS 40.0%
AAGCAAGAGTTCTTGACCAA+AGG + chr8_4:5174129-5174148 Msa1308820:CDS 40.0%
GAGTTCTTGACCAAAGGATA+AGG + chr8_4:5174135-5174154 Msa1308820:CDS 40.0%
GTGAAGTAGACCTTATCCTT+TGG - chr8_4:5174148-5174167 None:intergenic 40.0%
!!! TTGTTTTGGCAGTCAAGAGA+AGG + chr8_4:5174457-5174476 Msa1308820:intron 40.0%
TTCTTTCCTCTGTTGCAACA+TGG - chr8_4:5174539-5174558 None:intergenic 40.0%
GAAAGAAGCAGCAATGAAGA+GGG + chr8_4:5174552-5174571 Msa1308820:CDS 40.0%
TTGTGAAAATGCTTGGGACA+AGG - chr8_4:5174584-5174603 None:intergenic 40.0%
CGATGAGTTCGACAAAATCT+AGG - chr8_4:5174837-5174856 None:intergenic 40.0%
! CAATTTTACTCCTCCAGAAG+TGG - chr8_4:5174897-5174916 None:intergenic 40.0%
!! TGCTTTGGTGTTTGGTTATG+CGG - chr8_4:5175185-5175204 None:intergenic 40.0%
CACAGATTTGGAGAAATGGT+AGG + chr8_4:5175235-5175254 Msa1308820:intron 40.0%
GACCAAAATGGCTAGATAGA+TGG + chr8_4:5175295-5175314 Msa1308820:CDS 40.0%
TACAAAGCCATGGGAGAATA+GGG + chr8_4:5175323-5175342 Msa1308820:CDS 40.0%
CAATGGAATGTCTTTGTGGT+TGG - chr8_4:5175473-5175492 None:intergenic 40.0%
GAAGCAATGGAATGTCTTTG+TGG - chr8_4:5175477-5175496 None:intergenic 40.0%
!! TTTTGGTGTGATCTATGGAG+TGG - chr8_4:5175501-5175520 None:intergenic 40.0%
CTCCATAGATCACACCAAAA+TGG + chr8_4:5175501-5175520 Msa1308820:CDS 40.0%
!! AGTGATGGATTGGTTGTGAA+CGG - chr8_4:5175529-5175548 None:intergenic 40.0%
TATCTTCTCGAACACAACGA+GGG - chr8_4:5175599-5175618 None:intergenic 40.0%
GGTACAAGTTCCCATTGTAA+TGG - chr8_4:5175668-5175687 None:intergenic 40.0%
TTGTACCAAAATAGTAGGGG+TGG + chr8_4:5175681-5175700 Msa1308820:CDS 40.0%
! TTGTTCCACCCCTACTATTT+TGG - chr8_4:5175689-5175708 None:intergenic 40.0%
TCGGTTGCTTGCATGTAATT+AGG - chr8_4:5175762-5175781 None:intergenic 40.0%
GATCCGGATCAGTAAAGAAA+AGG + chr8_4:5175850-5175869 Msa1308820:CDS 40.0%
CATTATAAGGATCAGGAGCA+GGG - chr8_4:5175884-5175903 None:intergenic 40.0%
ACATTATAAGGATCAGGAGC+AGG - chr8_4:5175885-5175904 None:intergenic 40.0%
GCTCCTGATCCTTATAATGT+AGG + chr8_4:5175885-5175904 Msa1308820:CDS 40.0%
CCTCCTACATTATAAGGATC+AGG - chr8_4:5175891-5175910 None:intergenic 40.0%
CCTGATCCTTATAATGTAGG+AGG + chr8_4:5175888-5175907 Msa1308820:CDS 40.0%
CCAACTCCTCCTACATTATA+AGG - chr8_4:5175897-5175916 None:intergenic 40.0%
CCTTATAATGTAGGAGGAGT+TGG + chr8_4:5175894-5175913 Msa1308820:CDS 40.0%
! AATGTAGGAGGAGTTGGTTA+TGG + chr8_4:5175900-5175919 Msa1308820:CDS 40.0%
AGCAGCATCACATTATGGAT+TGG + chr8_4:5175971-5175990 Msa1308820:CDS 40.0%
! TCTTGTTGTACTGAGAGTCT+CGG + chr8_4:5176014-5176033 Msa1308820:CDS 40.0%
GCAAGTACTATGTTGTTGCT+AGG + chr8_4:5176297-5176316 Msa1308820:three_prime_UTR 40.0%
TAGGATGCCAAGATAAATGC+TGG + chr8_4:5176365-5176384 Msa1308820:three_prime_UTR 40.0%
! GGATTTTTGTGGCATGAAGG+TGG - chr8_4:5172390-5172409 None:intergenic 45.0%
TTTGTGAGTGAGTGAGTGAG+AGG - chr8_4:5172488-5172507 None:intergenic 45.0%
AAAGGTTCAACTTGGTTGGG+TGG - chr8_4:5172908-5172927 None:intergenic 45.0%
TGAGAAACATGCTCTTGCTG+TGG + chr8_4:5173427-5173446 Msa1308820:CDS 45.0%
AGTCAACCTTGCAACTTCCA+CGG - chr8_4:5173495-5173514 None:intergenic 45.0%
CTGCGAGTTTGACGAAATCA+CGG + chr8_4:5173624-5173643 Msa1308820:intron 45.0%
AAGTGCTCTTCTTGCCTTCA+AGG - chr8_4:5174003-5174022 None:intergenic 45.0%
! AACACTAGCAGTGTCACTGA+AGG - chr8_4:5174192-5174211 None:intergenic 45.0%
TCAGTGACACTGCTAGTGTT+TGG + chr8_4:5174192-5174211 Msa1308820:CDS 45.0%
CAGTGACACTGCTAGTGTTT+GGG + chr8_4:5174193-5174212 Msa1308820:CDS 45.0%
TTAAGGCCATGTTGCAACAG+AGG + chr8_4:5174530-5174549 Msa1308820:CDS 45.0%
GGAAAGAAGCAGCAATGAAG+AGG + chr8_4:5174551-5174570 Msa1308820:CDS 45.0%
! GTCCCAAGCATTTTCACAAC+AGG + chr8_4:5174585-5174604 Msa1308820:CDS 45.0%
TGCATGTGTCTTTCCACTTC+TGG + chr8_4:5174881-5174900 Msa1308820:intron 45.0%
ATGTGTCTTTCCACTTCTGG+AGG + chr8_4:5174884-5174903 Msa1308820:intron 45.0%
TGGATCTTCTCGAGCAGAAT+TGG + chr8_4:5174950-5174969 Msa1308820:intron 45.0%
ATGGATGGCTACAAAGCCAT+GGG + chr8_4:5175314-5175333 Msa1308820:CDS 45.0%
CTACAAAGCCATGGGAGAAT+AGG + chr8_4:5175322-5175341 Msa1308820:CDS 45.0%
ACAAAGCCATGGGAGAATAG+GGG + chr8_4:5175324-5175343 Msa1308820:CDS 45.0%
!! TGTCTTTGTGGTTGGTGATG+TGG - chr8_4:5175465-5175484 None:intergenic 45.0%
TCTATGGAGTGGTGAAGCAA+TGG - chr8_4:5175490-5175509 None:intergenic 45.0%
! GGTTGTGAACGGATCCATTT+TGG - chr8_4:5175518-5175537 None:intergenic 45.0%
! GGTCTTGATTTTGCTGGAGA+CGG - chr8_4:5175555-5175574 None:intergenic 45.0%
CTTGACGATCGGACTTGAAT+AGG - chr8_4:5175576-5175595 None:intergenic 45.0%
CTATCTTCTCGAACACAACG+AGG - chr8_4:5175600-5175619 None:intergenic 45.0%
!! CCTTAAGCTTGGTGTTTGAG+AGG - chr8_4:5175637-5175656 None:intergenic 45.0%
CCTCTCAAACACCAAGCTTA+AGG + chr8_4:5175634-5175653 Msa1308820:CDS 45.0%
AGTAGGGGTGGAACAAGTAA+TGG + chr8_4:5175693-5175712 Msa1308820:CDS 45.0%
! TGCAATGACTGCGGAGTATT+CGG + chr8_4:5176121-5176140 Msa1308820:three_prime_UTR 45.0%
CATACGGCCAGCATTTATCT+TGG - chr8_4:5176375-5176394 None:intergenic 45.0%
TAAATGCTGGCCGTATGTTC+TGG + chr8_4:5176378-5176397 Msa1308820:three_prime_UTR 45.0%
ATTGTTCAACACCCCAAGTG+AGG - chr8_4:5176408-5176427 None:intergenic 45.0%
!!! TTTACATAATAATAAAATTT+TGG + chr8_4:5172434-5172453 Msa1308820:five_prime_UTR 5.0%
!!! TATTATATATTCATTTTTTT+TGG + chr8_4:5172688-5172707 Msa1308820:five_prime_UTR 5.0%
GTGCTACAAGTGTGGCTTCA+AGG + chr8_4:5173393-5173412 Msa1308820:CDS 50.0%
TGTGGCTTCAAGGACAGATC+AGG + chr8_4:5173403-5173422 Msa1308820:CDS 50.0%
! ACATGCTCTTGCTGTGGCTA+TGG + chr8_4:5173433-5173452 Msa1308820:CDS 50.0%
!! AAGAGCACTTCGTGCGCTTA+AGG + chr8_4:5174013-5174032 Msa1308820:CDS 50.0%
! AGAGCACTTCGTGCGCTTAA+GGG + chr8_4:5174014-5174033 Msa1308820:CDS 50.0%
! CTGCTAGTGTTTGGGATTCG+CGG + chr8_4:5174201-5174220 Msa1308820:CDS 50.0%
GATGGATGGCTACAAAGCCA+TGG + chr8_4:5175313-5175332 Msa1308820:CDS 50.0%
!! GAGACGGAGTAGTGATGGAT+TGG - chr8_4:5175539-5175558 None:intergenic 50.0%
! TTGGTGTTTGAGAGGTGTGG+TGG - chr8_4:5175629-5175648 None:intergenic 50.0%
!! AGCTTGGTGTTTGAGAGGTG+TGG - chr8_4:5175632-5175651 None:intergenic 50.0%
AATTCGAGCCTTGGCAGACT+CGG - chr8_4:5175781-5175800 None:intergenic 50.0%
AGTCTGCCAAGGCTCGAATT+CGG + chr8_4:5175781-5175800 Msa1308820:CDS 50.0%
ACTCCAGAGAGGGATAGATC+CGG + chr8_4:5175834-5175853 Msa1308820:CDS 50.0%
TGTTGTACTGAGAGTCTCGG+TGG + chr8_4:5176017-5176036 Msa1308820:CDS 50.0%
GAGGTTTCACCTTCGTCAAC+CGG + chr8_4:5176041-5176060 Msa1308820:CDS 50.0%
CTCATCTCAACCACCGTCTA+AGG - chr8_4:5176070-5176089 None:intergenic 50.0%
GTGAGGTAGGCCAGAACATA+CGG - chr8_4:5176391-5176410 None:intergenic 50.0%
ATGTTCTGGCCTACCTCACT+TGG + chr8_4:5176392-5176411 Msa1308820:three_prime_UTR 50.0%
TGTTCTGGCCTACCTCACTT+GGG + chr8_4:5176393-5176412 Msa1308820:three_prime_UTR 50.0%
TTCAACACCCCAAGTGAGGT+AGG - chr8_4:5176404-5176423 None:intergenic 50.0%
!! ACAGCAGTGGGAGTGTCAGA+AGG - chr8_4:5172367-5172386 None:intergenic 55.0%
GCATGAAGGTGGACAGCAGT+GGG - chr8_4:5172379-5172398 None:intergenic 55.0%
TGAGTGAGTGAGAGGAGGAG+GGG - chr8_4:5172480-5172499 None:intergenic 55.0%
GTGAGTGAGTGAGAGGAGGA+GGG - chr8_4:5172481-5172500 None:intergenic 55.0%
AGTGAGTGAGTGAGAGGAGG+AGG - chr8_4:5172482-5172501 None:intergenic 55.0%
GTGAGTGAGTGAGTGAGAGG+AGG - chr8_4:5172485-5172504 None:intergenic 55.0%
TGTGGCTATGGCTACAGCTG+AGG + chr8_4:5173445-5173464 Msa1308820:CDS 55.0%
AAGCTGCCGTGGAAGTTGCA+AGG + chr8_4:5173486-5173505 Msa1308820:CDS 55.0%
GAGCACTTCGTGCGCTTAAG+GGG + chr8_4:5174015-5174034 Msa1308820:CDS 55.0%
AGCCATGGGAGAATAGGGGA+AGG + chr8_4:5175328-5175347 Msa1308820:CDS 55.0%
GCCATGGGAGAATAGGGGAA+GGG + chr8_4:5175329-5175348 Msa1308820:CDS 55.0%
TGCTGGAGACGGAGTAGTGA+TGG - chr8_4:5175544-5175563 None:intergenic 55.0%
CACAACGAGGGCTTGACGAT+CGG - chr8_4:5175587-5175606 None:intergenic 55.0%
!! AAAGGCCATCGACTCCAGAG+AGG + chr8_4:5175823-5175842 Msa1308820:CDS 55.0%
!! AAGGCCATCGACTCCAGAGA+GGG + chr8_4:5175824-5175843 Msa1308820:CDS 55.0%
!! CTATCCCTCTCTGGAGTCGA+TGG - chr8_4:5175831-5175850 None:intergenic 55.0%
GATCCGGATCTATCCCTCTC+TGG - chr8_4:5175840-5175859 None:intergenic 55.0%
! CTAAGGTCACCGGTTGACGA+AGG - chr8_4:5176053-5176072 None:intergenic 55.0%
CGTCAACCGGTGACCTTAGA+CGG + chr8_4:5176054-5176073 Msa1308820:CDS 55.0%
! CAACCACCGTCTAAGGTCAC+CGG - chr8_4:5176063-5176082 None:intergenic 55.0%
CAACGGCTCTGCAATGACTG+CGG + chr8_4:5176112-5176131 Msa1308820:three_prime_UTR 55.0%
GTTCTGGCCTACCTCACTTG+GGG + chr8_4:5176394-5176413 Msa1308820:three_prime_UTR 55.0%
GGCATGAAGGTGGACAGCAG+TGG - chr8_4:5172380-5172399 None:intergenic 60.0%
GAGTGAGTGAGAGGAGGAGG+GGG - chr8_4:5172479-5172498 None:intergenic 60.0%
AGCTACTGCTCAAGCTGCCG+TGG + chr8_4:5173475-5173494 Msa1308820:CDS 60.0%
GCCCTTCCCCTATTCTCCCA+TGG - chr8_4:5175333-5175352 None:intergenic 60.0%
GCAAGCAACCGAGTCTGCCA+AGG + chr8_4:5175770-5175789 Msa1308820:CDS 60.0%
CTGCGACCGAATTCGAGCCT+TGG - chr8_4:5175790-5175809 None:intergenic 60.0%
GGAGTCGATGGCCTTTGCCT+TGG - chr8_4:5175819-5175838 None:intergenic 60.0%
TACTGAGAGTCTCGGTGGCG+AGG + chr8_4:5176022-5176041 Msa1308820:CDS 60.0%
CAACCGGTGACCTTAGACGG+TGG + chr8_4:5176057-5176076 Msa1308820:CDS 60.0%
TTCGGTCGCAGAGTGCGCCA+AGG + chr8_4:5175799-5175818 Msa1308820:CDS 65.0%
CGCAGAGTGCGCCAAGGCAA+AGG + chr8_4:5175805-5175824 Msa1308820:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 5172323 5176570 5172323 ID=Msa1308820;Name=Msa1308820
chr8_4 mRNA 5172323 5176570 5172323 ID=Msa1308820-mRNA-1;Parent=Msa1308820;Name=Msa1308820-mRNA-1;_AED=0.01;_eAED=0.01;_QI=701|1|1|1|1|1|5|482|552
chr8_4 exon 5172323 5173152 5172323 ID=Msa1308820-mRNA-1:exon:1556;Parent=Msa1308820-mRNA-1
chr8_4 exon 5173290 5173583 5173290 ID=Msa1308820-mRNA-1:exon:1557;Parent=Msa1308820-mRNA-1
chr8_4 exon 5174008 5174256 5174008 ID=Msa1308820-mRNA-1:exon:1558;Parent=Msa1308820-mRNA-1
chr8_4 exon 5174469 5174606 5174469 ID=Msa1308820-mRNA-1:exon:1559;Parent=Msa1308820-mRNA-1
chr8_4 exon 5175240 5176570 5175240 ID=Msa1308820-mRNA-1:exon:1560;Parent=Msa1308820-mRNA-1
chr8_4 five_prime_UTR 5172323 5173023 5172323 ID=Msa1308820-mRNA-1:five_prime_utr;Parent=Msa1308820-mRNA-1
chr8_4 CDS 5173024 5173152 5173024 ID=Msa1308820-mRNA-1:cds;Parent=Msa1308820-mRNA-1
chr8_4 CDS 5173290 5173583 5173290 ID=Msa1308820-mRNA-1:cds;Parent=Msa1308820-mRNA-1
chr8_4 CDS 5174008 5174256 5174008 ID=Msa1308820-mRNA-1:cds;Parent=Msa1308820-mRNA-1
chr8_4 CDS 5174469 5174606 5174469 ID=Msa1308820-mRNA-1:cds;Parent=Msa1308820-mRNA-1
chr8_4 CDS 5175240 5176088 5175240 ID=Msa1308820-mRNA-1:cds;Parent=Msa1308820-mRNA-1
chr8_4 three_prime_UTR 5176089 5176570 5176089 ID=Msa1308820-mRNA-1:three_prime_utr;Parent=Msa1308820-mRNA-1
Gene Sequence

>Msa1308820

ATGGGGAAGAAAGGTAGTAATTCATGGTTAACTGCTGTGAAAAGAGCATTCAGATCTCCAACAAAAGATAGTGAAAAAAGAAGTGGTAGAAGAAGAGAAGATTGTGATCAAGAAGAAGATGAAGAAAAGAAGAGAGAAAAAAGAAGGTGGATTTTTAGGAAACCAGTGAATCATGAAACAGTGAATAATACACAACAAAGTACACCAACAAAGTTGAAGCATGATGTTGTTAGTGCTACAAGTGTGGCTTCAAGGACAGATCAGGATGAGAAACATGCTCTTGCTGTGGCTATGGCTACAGCTGAGGCAGCAAGAGCTACTGCTCAAGCTGCCGTGGAAGTTGCAAGGTTGACTAAGAGTTCAAATCATGCTAGGGAGCATTATGCTGCTGTTGTGATTCAAACTTCTTTTAGAGGTTATTTGGCAAGAAGAGCACTTCGTGCGCTTAAGGGGTTAGTGAAGTTGCAAGCTTTAGTGAGAGGTCACAATGTTAGGAAGCAAGCAAAGATGACACTTAGGTGCATGCAAGCTCTTGTTAGAGTACAAGCAAGAGTTCTTGACCAAAGGATAAGGTCTACTTCACATGAAGGAAGTAGAAAATCAACCTTCAGTGACACTGCTAGTGTTTGGGATTCGCGGTATCTTCAAGACATTTCAGATAGGAAATCAATATCAAGAGAAGGAAGTAGTATTGCAGATGATTGGGATGATCGTCCACACACAGTTGAAGAAGTTAAGGCCATGTTGCAACAGAGGAAAGAAGCAGCAATGAAGAGGGAAAAGTCCTTGTCCCAAGCATTTTCACAACAGATTTGGAGAAATGGTAGGACATCATCAATTGGAAATGAAGATGAACTTGAAGAGAGACCAAAATGGCTAGATAGATGGATGGCTACAAAGCCATGGGAGAATAGGGGAAGGGCTTCAACCGATCAAAGAGACTCTATTAAGACTGTGGAAGTCGACACATCTCAACCTTATTCATATCTAGGAGCAAATTACAGAAGATCACATCCAAATTATCAATATAATCCACATCACCAACCACAAAGACATTCCATTGCTTCACCACTCCATAGATCACACCAAAATGGATCCGTTCACAACCAATCCATCACTACTCCGTCTCCAGCAAAATCAAGACCTATTCAAGTCCGATCGTCAAGCCCTCGTTGTGTTCGAGAAGATAGAAGCTACCACCACACCTCTCAAACACCAAGCTTAAGGTCTAATTACCATTACAATGGGAACTTGTACCAAAATAGTAGGGGTGGAACAAGTAATGGTAGTTGTAACAGTATTGCTTCTACTACTGCTTCAGCTACATTGCCTAATTACATGCAAGCAACCGAGTCTGCCAAGGCTCGAATTCGGTCGCAGAGTGCGCCAAGGCAAAGGCCATCGACTCCAGAGAGGGATAGATCCGGATCAGTAAAGAAAAGGCTTTCTTTCCCTGCTCCTGATCCTTATAATGTAGGAGGAGTTGGTTATGGAAATTATGGACATAGTTTAAGGAGTCCAAGTTTCAAGAGTGTGAATGTAGCAGCATCACATTATGGATTGGAGCAAAATTCCAATTACTCTTCTTGTTGTACTGAGAGTCTCGGTGGCGAGGTTTCACCTTCGTCAACCGGTGACCTTAGACGGTGGTTGAGATGA
Protein sequence

>Msa1308820

MGKKGSNSWLTAVKRAFRSPTKDSEKRSGRRREDCDQEEDEEKKREKRRWIFRKPVNHETVNNTQQSTPTKLKHDVVSATSVASRTDQDEKHALAVAMATAEAARATAQAAVEVARLTKSSNHAREHYAAVVIQTSFRGYLARRALRALKGLVKLQALVRGHNVRKQAKMTLRCMQALVRVQARVLDQRIRSTSHEGSRKSTFSDTASVWDSRYLQDISDRKSISREGSSIADDWDDRPHTVEEVKAMLQQRKEAAMKREKSLSQAFSQQIWRNGRTSSIGNEDELEERPKWLDRWMATKPWENRGRASTDQRDSIKTVEVDTSQPYSYLGANYRRSHPNYQYNPHHQPQRHSIASPLHRSHQNGSVHNQSITTPSPAKSRPIQVRSSSPRCVREDRSYHHTSQTPSLRSNYHYNGNLYQNSRGGTSNGSCNSIASTTASATLPNYMQATESAKARIRSQSAPRQRPSTPERDRSGSVKKRLSFPAPDPYNVGGVGYGNYGHSLRSPSFKSVNVAASHYGLEQNSNYSSCCTESLGGEVSPSSTGDLRRWLR*