AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0078500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078500 MtrunA17_Chr1g0189111 97.492 319 8 0 1 319 1 319 0.0 644
Msa0078500 MtrunA17_Chr2g0321511 56.230 313 132 3 10 319 6 316 9.92e-126 365
Msa0078500 MtrunA17_Chr4g0043851 52.038 319 149 2 5 319 3 321 1.15e-121 355
Msa0078500 MtrunA17_Chr4g0026321 56.701 291 125 1 30 319 28 318 2.92e-121 353
Msa0078500 MtrunA17_Chr2g0285901 48.562 313 150 3 17 319 1 312 3.36e-105 313
Msa0078500 MtrunA17_Chr4g0003281 46.483 327 167 3 1 319 4 330 1.38e-100 301
Msa0078500 MtrunA17_Chr4g0064021 48.276 290 148 2 32 319 27 316 2.36e-92 280
Msa0078500 MtrunA17_Chr1g0155001 46.735 291 152 3 31 318 24 314 2.20e-90 276
Msa0078500 MtrunA17_Chr3g0144531 44.516 310 168 3 13 318 8 317 1.78e-88 272
Msa0078500 MtrunA17_Chr1g0177171 47.635 296 151 4 27 319 34 328 7.84e-88 271
Msa0078500 MtrunA17_Chr4g0034901 43.548 310 171 3 13 318 9 318 2.27e-87 270
Msa0078500 MtrunA17_Chr3g0119541 46.488 299 156 4 24 319 19 316 8.39e-86 262
Msa0078500 MtrunA17_Chr5g0439251 48.148 297 150 4 26 319 20 315 1.54e-85 265
Msa0078500 MtrunA17_Chr1g0146831 45.085 295 153 5 29 319 29 318 8.96e-83 255
Msa0078500 MtrunA17_Chr8g0389741 45.392 293 153 4 31 319 28 317 6.53e-81 254
Msa0078500 MtrunA17_Chr6g0487261 41.853 313 178 4 10 319 5 316 1.31e-79 251
Msa0078500 MtrunA17_Chr7g0223961 41.751 297 164 7 26 319 18 308 8.17e-75 236
Msa0078500 MtrunA17_Chr8g0339191 41.346 312 176 5 13 318 11 321 1.32e-74 236
Msa0078500 MtrunA17_Chr4g0043511 42.193 301 169 5 22 319 53 351 5.37e-68 222
Msa0078500 MtrunA17_Chr7g0234821 39.456 294 171 5 32 319 27 319 4.81e-67 217
Msa0078500 MtrunA17_Chr4g0046311 37.261 314 188 5 14 319 6 318 9.65e-67 216
Msa0078500 MtrunA17_Chr8g0378621 39.456 294 172 4 31 319 26 318 1.01e-66 217
Msa0078500 MtrunA17_Chr3g0121271 35.783 313 191 5 16 319 7 318 8.56e-64 209
Msa0078500 MtrunA17_Chr4g0075031 39.116 294 173 4 31 319 45 337 1.13e-62 207
Msa0078500 MtrunA17_Chr4g0038971 37.255 306 179 8 15 319 6 299 4.05e-62 200
Msa0078500 MtrunA17_Chr7g0250301 36.082 291 176 6 31 319 8 290 9.22e-60 194
Msa0078500 MtrunA17_Chr4g0039011 37.540 309 182 7 11 319 22 319 2.07e-59 194
Msa0078500 MtrunA17_Chr1g0161011 38.851 296 169 5 31 318 24 315 1.19e-58 196
Msa0078500 MtrunA17_Chr2g0294601 36.050 319 195 7 4 319 31 343 1.55e-58 193
Msa0078500 MtrunA17_Chr5g0434591 35.806 310 191 5 16 318 6 314 2.26e-58 195
Msa0078500 MtrunA17_Chr3g0110371 34.824 313 197 5 12 318 4 315 5.98e-58 194
Msa0078500 MtrunA17_Chr4g0038941 37.755 294 171 6 26 319 47 328 4.65e-57 188
Msa0078500 MtrunA17_Chr4g0038981 37.255 306 179 9 15 319 6 299 8.16e-57 186
Msa0078500 MtrunA17_Chr4g0038961 36.275 306 182 9 15 319 6 299 6.80e-56 184
Msa0078500 MtrunA17_Chr2g0294591 37.124 299 178 7 24 319 23 314 6.36e-55 182
Msa0078500 MtrunA17_Chr7g0250321 34.737 285 177 5 13 296 10 286 1.10e-54 180
Msa0078500 MtrunA17_Chr4g0039071 36.177 293 180 5 28 319 22 308 2.05e-52 177
Msa0078500 MtrunA17_Chr4g0039021 36.882 263 155 7 59 319 9 262 3.76e-50 168
Msa0078500 MtrunA17_Chr2g0290821 34.653 303 185 10 24 319 22 318 7.25e-48 167
Msa0078500 MtrunA17_Chr1g0174261 37.786 262 154 6 60 319 1 255 9.11e-48 164
Msa0078500 MtrunA17_Chr7g0253091 34.000 300 181 9 29 319 24 315 3.14e-47 165
Msa0078500 MtrunA17_Chr1g0148391 33.333 315 193 11 13 319 9 314 3.63e-47 165
Msa0078500 MtrunA17_Chr3g0138741 34.062 320 195 11 8 319 8 319 1.08e-46 164
Msa0078500 MtrunA17_Chr6g0461601 31.646 316 197 9 15 319 10 317 3.83e-46 162
Msa0078500 MtrunA17_Chr8g0383311 34.122 296 183 9 30 319 42 331 1.15e-45 161
Msa0078500 MtrunA17_Chr4g0062771 32.298 322 200 11 8 319 8 321 1.30e-42 153
Msa0078500 MtrunA17_Chr4g0069801 33.022 321 198 12 7 319 5 316 1.01e-41 151
Msa0078500 MtrunA17_Chr4g0076581 32.051 312 199 10 14 319 17 321 1.25e-39 145
Msa0078500 MtrunA17_Chr3g0129341 32.542 295 187 10 30 319 33 320 3.35e-38 141
Msa0078500 MtrunA17_Chr5g0436361 35.125 279 170 9 47 319 53 326 3.48e-38 139
Msa0078500 MtrunA17_Chr2g0306311 29.762 336 194 11 19 319 18 346 9.96e-36 135
Msa0078500 MtrunA17_Chr3g0105981 29.801 302 198 9 24 319 20 313 3.80e-28 113
Msa0078500 MtrunA17_Chr2g0294581 31.925 213 129 6 24 227 69 274 3.61e-21 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078500 AT1G32860.1 64.127 315 109 2 8 319 7 320 4.81e-146 418
Msa0078500 AT5G42100.2 64.966 294 99 2 30 319 26 319 8.57e-140 402
Msa0078500 AT5G42100.1 64.966 294 99 2 30 319 26 319 1.37e-139 402
Msa0078500 AT2G27500.2 59.661 295 117 2 27 319 25 319 2.18e-129 374
Msa0078500 AT2G27500.1 59.661 295 117 2 27 319 25 319 3.13e-129 374
Msa0078500 AT1G30080.2 52.525 297 140 1 24 319 29 325 4.14e-112 330
Msa0078500 AT1G30080.1 52.525 297 140 1 24 319 29 325 8.19e-112 330
Msa0078500 AT4G18340.1 47.403 308 161 1 13 319 16 323 2.22e-108 321
Msa0078500 AT4G18340.2 47.403 308 161 1 13 319 16 323 2.73e-108 320
Msa0078500 AT2G27500.3 60.166 241 94 2 81 319 1 241 4.74e-103 305
Msa0078500 AT3G15800.1 51.890 291 137 3 32 319 44 334 5.76e-101 302
Msa0078500 AT2G26600.1 50.345 290 142 2 32 319 35 324 2.79e-98 295
Msa0078500 AT5G42720.1 46.602 309 159 4 16 318 8 316 1.67e-90 277
Msa0078500 AT4G26830.1 46.129 310 161 5 13 319 8 314 1.17e-89 275
Msa0078500 AT2G05790.1 49.147 293 145 4 30 319 24 315 1.18e-88 273
Msa0078500 AT4G26830.2 47.315 298 152 4 25 319 23 318 1.56e-88 273
Msa0078500 AT4G34480.2 47.586 290 150 2 31 318 26 315 5.51e-87 265
Msa0078500 AT5G24318.4 44.667 300 158 5 24 319 22 317 2.77e-86 264
Msa0078500 AT5G55180.2 45.548 292 155 4 31 319 27 317 6.91e-85 263
Msa0078500 AT5G24318.2 44.667 300 158 5 24 319 22 317 1.23e-84 262
Msa0078500 AT4G34480.1 47.586 290 150 2 31 318 26 315 1.29e-84 264
Msa0078500 AT5G55180.1 45.548 292 155 4 31 319 27 317 1.55e-84 262
Msa0078500 AT5G24318.1 44.667 300 158 5 24 319 22 317 2.44e-84 262
Msa0078500 AT2G16230.1 45.704 291 156 2 31 319 25 315 2.01e-83 261
Msa0078500 AT5G56590.1 42.808 292 164 3 31 319 26 317 1.19e-76 243
Msa0078500 AT3G07320.1 44.369 293 156 5 32 318 26 317 2.11e-76 241
Msa0078500 AT2G26600.2 50.893 224 109 1 97 319 7 230 5.12e-76 235
Msa0078500 AT4G29360.1 43.689 309 168 4 13 319 12 316 1.28e-73 236
Msa0078500 AT4G14080.1 40.694 317 174 6 8 319 27 334 2.12e-72 231
Msa0078500 AT4G29360.2 44.482 299 160 4 23 319 32 326 3.73e-72 233
Msa0078500 AT3G13560.4 41.980 293 164 4 31 318 26 317 1.13e-70 228
Msa0078500 AT3G13560.1 41.980 293 164 4 31 318 26 317 1.13e-70 228
Msa0078500 AT3G13560.2 41.980 293 164 4 31 318 26 317 1.13e-70 228
Msa0078500 AT3G13560.5 41.980 293 164 4 31 318 26 317 1.13e-70 228
Msa0078500 AT3G13560.3 41.980 293 164 4 31 318 26 317 1.13e-70 228
Msa0078500 AT2G01630.2 40.956 293 167 4 31 318 23 314 6.10e-68 218
Msa0078500 AT1G11820.1 39.933 298 173 4 27 319 40 336 8.73e-68 217
Msa0078500 AT3G23770.1 41.000 300 168 5 28 319 34 332 9.88e-68 219
Msa0078500 AT2G01630.3 40.956 293 167 4 31 318 23 314 2.29e-67 218
Msa0078500 AT1G11820.3 39.933 298 173 4 27 319 40 336 1.53e-66 216
Msa0078500 AT2G01630.1 40.956 293 167 4 31 318 23 314 2.14e-66 216
Msa0078500 AT1G11820.2 40.136 294 170 4 31 319 44 336 3.65e-66 216
Msa0078500 AT3G61810.1 36.808 307 179 5 20 319 44 342 3.03e-64 207
Msa0078500 AT4G16260.1 36.774 310 186 5 13 319 4 306 3.50e-64 206
Msa0078500 AT3G57270.1 39.655 290 165 6 31 318 27 308 2.09e-62 202
Msa0078500 AT3G46570.1 42.034 295 160 9 31 319 27 316 1.50e-61 200
Msa0078500 AT3G55430.1 38.436 307 175 10 18 319 17 314 1.12e-59 197
Msa0078500 AT2G39640.1 36.709 316 191 5 5 319 4 311 1.43e-58 197
Msa0078500 AT4G16260.2 36.749 283 171 4 38 319 2 277 9.50e-58 189
Msa0078500 AT1G66250.1 36.789 299 183 4 25 318 26 323 1.46e-57 194
Msa0078500 AT4G34480.3 49.505 202 101 1 118 318 3 204 2.56e-56 187
Msa0078500 AT1G33220.1 36.120 299 180 7 22 317 25 315 4.73e-56 185
Msa0078500 AT5G20330.1 36.120 299 180 7 22 317 25 315 6.19e-55 182
Msa0078500 AT5G20340.1 36.242 298 179 7 23 317 35 324 8.96e-55 182
Msa0078500 AT5G20390.1 35.127 316 194 7 5 317 7 314 4.89e-54 180
Msa0078500 AT1G77780.1 34.505 313 191 8 13 319 2 306 1.63e-50 171
Msa0078500 AT3G57260.2 33.333 309 193 7 13 319 29 326 5.05e-50 170
Msa0078500 AT3G57260.1 33.333 309 193 7 13 319 13 310 5.10e-50 169
Msa0078500 AT5G24318.3 45.055 182 94 3 140 319 1 178 3.11e-47 162
Msa0078500 AT5G58090.1 35.000 300 181 10 27 319 18 310 5.43e-47 165
Msa0078500 AT3G57240.1 35.172 290 174 9 31 319 35 311 5.21e-46 159
Msa0078500 AT1G77790.1 31.269 323 210 7 1 319 1 315 2.37e-45 157
Msa0078500 AT4G31140.1 32.258 310 198 9 16 319 12 315 2.86e-44 158
Msa0078500 AT5G20560.1 32.686 309 197 8 11 316 15 315 6.88e-42 148
Msa0078500 AT3G55780.1 33.569 283 178 9 44 319 48 327 9.98e-42 149
Msa0078500 AT1G64760.1 32.588 313 197 9 14 319 8 313 1.18e-41 151
Msa0078500 AT1G64760.2 32.588 313 197 9 14 319 8 313 1.18e-41 151
Msa0078500 AT4G17180.1 32.881 295 183 9 30 319 24 308 2.08e-40 147
Msa0078500 AT2G19440.2 32.886 298 186 9 29 319 19 309 3.00e-40 147
Msa0078500 AT2G19440.1 32.886 298 186 9 29 319 19 309 3.00e-40 147
Msa0078500 AT5G18220.1 30.573 314 200 8 14 319 13 316 6.30e-40 146
Msa0078500 AT5G20870.1 32.812 320 184 14 19 319 18 325 3.12e-39 144
Msa0078500 AT3G24330.1 30.721 319 201 12 11 319 21 329 4.87e-37 139
Msa0078500 AT5G64790.1 31.126 302 196 9 24 319 21 316 6.82e-37 138
Msa0078500 AT3G04010.1 31.975 319 191 12 13 319 17 321 1.43e-36 137
Msa0078500 AT5G58480.1 30.233 301 198 8 25 319 20 314 1.28e-33 129

Find 142 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGGTTATTTGGCCATTTC+TGG 0.048168 1_2:-56099433 Msa0078500:three_prime_UTR
GATGCCCCTTGTATCTTCTT+TGG 0.185133 1_2:+56098938 None:intergenic
TTGTGACAGTGATTTGTTTA+TGG 0.189858 1_2:+56100126 None:intergenic
TTATCCTTACTTTGCTTATA+AGG 0.190578 1_2:-56099970 Msa0078500:CDS
CAAAATGAGAACCAATAAAA+TGG 0.196069 1_2:+56098073 None:intergenic
AACTTCTTCCTCCGGTTATT+TGG 0.209849 1_2:-56099443 Msa0078500:three_prime_UTR
CCAACTTCTGAGAGGAATTA+TGG 0.228803 1_2:-56099604 Msa0078500:three_prime_UTR
GGGAATCCTGTTTATAATCT+TGG 0.235486 1_2:-56099565 Msa0078500:three_prime_UTR
CAAGAAAGGTGAACCAGTTT+TGG 0.236223 1_2:+56100001 None:intergenic
GCGAAAGCTTTGAGAACTTT+TGG 0.247516 1_2:+56100383 None:intergenic
TCCATGCTTGAGCTTGTTTA+GGG 0.266792 1_2:+56100300 None:intergenic
ATCCATGCTTGAGCTTGTTT+AGG 0.272003 1_2:+56100299 None:intergenic
CATGATGAGAAAGGAGTAAA+AGG 0.273853 1_2:-56098890 Msa0078500:intron
TAATGGCACGGTGATATTAA+TGG 0.281195 1_2:-56100827 Msa0078500:five_prime_UTR
CACACTGCACTCATAGTTCT+TGG 0.288580 1_2:-56100152 Msa0078500:CDS
TTTGTATGACCCAGATTTAA+TGG 0.317738 1_2:-56100844 Msa0078500:five_prime_UTR
TATCACCGTGCCATTAAATC+TGG 0.326421 1_2:+56100834 None:intergenic
GGATCAACCATTCCTTGGTT+TGG 0.327470 1_2:+56099906 None:intergenic
TTAAGCTTTCAATCCAAAAC+TGG 0.338481 1_2:-56100014 Msa0078500:CDS
TATTCTGCGCTTGGAGCATT+AGG 0.348295 1_2:-56099831 Msa0078500:CDS
ACTTCTTATCCACCTTCTTC+CGG 0.351800 1_2:-56100077 Msa0078500:CDS
TCGAATGATGGTGGTAATAA+TGG 0.352748 1_2:-56099502 Msa0078500:three_prime_UTR
GTGTGAACACTTTGCATTGC+TGG 0.359796 1_2:+56100170 None:intergenic
GAAAATGCGAGAAAGTATAA+TGG 0.372959 1_2:-56099729 Msa0078500:CDS
TAATCTAACGATATTTGCTT+AGG 0.376376 1_2:+56099942 None:intergenic
AACTTCAAAATCATCAATAA+TGG 0.381024 1_2:+56098990 None:intergenic
GGTGGTAATAATGGTACTAT+TGG 0.395208 1_2:-56099493 Msa0078500:three_prime_UTR
TAAATATTCAAATCAACTTC+AGG 0.401550 1_2:+56099663 None:intergenic
AGTAAATAATCTGGCATGGC+GGG 0.402195 1_2:+56098108 None:intergenic
GCAACAAAATCTGAATCAAT+TGG 0.409686 1_2:-56100509 Msa0078500:CDS
CCGGTGCATATTTCTGAAAC+AGG 0.410476 1_2:-56099795 Msa0078500:CDS
TAATATGATATGATTTGATA+TGG 0.412003 1_2:-56098031 Msa0078500:three_prime_UTR
AAAATGCGAGAAAGTATAAT+GGG 0.428212 1_2:-56099728 Msa0078500:CDS
AATGAATGAGAGTGGGTTGA+GGG 0.430072 1_2:+56100731 None:intergenic
TCCAGAAATGGCCAAATAAC+CGG 0.432218 1_2:+56099432 None:intergenic
GACGCTGTTTATTCTGCGCT+TGG 0.433976 1_2:-56099840 Msa0078500:CDS
ACATAAATAAGTAAATAATC+TGG 0.434217 1_2:+56098099 None:intergenic
TATATCAGAACGAAAAGTTC+CGG 0.437960 1_2:+56100058 None:intergenic
GAGAGTGGGTTGAGGGAAGG+TGG 0.439333 1_2:+56100738 None:intergenic
TTAATGTCTCATGCCGGTCT+AGG 0.443906 1_2:-56098161 Msa0078500:three_prime_UTR
CATGTATGGATCTGAGGATT+GGG 0.445535 1_2:+56100688 None:intergenic
ATAGAATTCATGGTAGGGCT+TGG 0.457900 1_2:-56100350 Msa0078500:CDS
GAGAATCCAAGATTATAAAC+AGG 0.458638 1_2:+56099559 None:intergenic
CAACACGGGTATAGAATTCA+TGG 0.462411 1_2:-56100360 Msa0078500:CDS
CATTTCTGGAGCTACTTCAC+TGG 0.464021 1_2:-56099419 Msa0078500:intron
AATCTAACGATATTTGCTTA+GGG 0.465599 1_2:+56099943 None:intergenic
GAAAAGGGAATGAATGAGAG+TGG 0.466093 1_2:+56100723 None:intergenic
CAGTGAAGTAGCTCCAGAAA+TGG 0.468492 1_2:+56099420 None:intergenic
CCATAATTCCTCTCAGAAGT+TGG 0.473439 1_2:+56099604 None:intergenic
GGGAATGTGAATAGTGAAAA+GGG 0.474637 1_2:+56100708 None:intergenic
AAAATGAGAACCAATAAAAT+GGG 0.477239 1_2:+56098074 None:intergenic
TGGGAATGTGAATAGTGAAA+AGG 0.478171 1_2:+56100707 None:intergenic
ATAATGAAATCCCAAGCATA+TGG 0.481891 1_2:+56099024 None:intergenic
GAATGAATGAGAGTGGGTTG+AGG 0.484057 1_2:+56100730 None:intergenic
TATGGATTGTTTAAGCCTGA+TGG 0.485868 1_2:-56099586 Msa0078500:three_prime_UTR
TCCACATTTCCTTCCTAGAC+CGG 0.488051 1_2:+56098148 None:intergenic
CATCCTCCAACTTCTTCCTC+CGG 0.488695 1_2:-56099451 Msa0078500:three_prime_UTR
CAGGAGAGATACCATATGCT+TGG 0.489037 1_2:-56099035 Msa0078500:three_prime_UTR
GAGTAAAGGCATGATGAGAA+AGG 0.489767 1_2:-56098899 Msa0078500:three_prime_UTR
ATCTGGCATGGCGGGGCACA+AGG 0.494737 1_2:+56098116 None:intergenic
TTTGGATTGAAAGCTTAAGA+TGG 0.495529 1_2:+56100019 None:intergenic
ATAAAGTTGAGTAGTAAGAA+AGG 0.495740 1_2:-56099699 Msa0078500:CDS
TCGAGTCCTCCTTCGAATGA+TGG 0.500599 1_2:-56099514 Msa0078500:three_prime_UTR
CATCAATAATGGTAGAAGAA+AGG 0.502229 1_2:+56099001 None:intergenic
TTATAAACAGGATTCCCATC+AGG 0.504478 1_2:+56099571 None:intergenic
GAACGAAAAGTTCCGGAAGA+AGG 0.505186 1_2:+56100065 None:intergenic
ATGGATTGTTTAAGCCTGAT+GGG 0.506000 1_2:-56099585 Msa0078500:three_prime_UTR
CCATGTATGGATCTGAGGAT+TGG 0.507597 1_2:+56100687 None:intergenic
GGAAGGAAATGTGGACATCA+AGG 0.508761 1_2:-56098140 Msa0078500:three_prime_UTR
TGAAGTTGCAATAGCGGTGC+CGG 0.509145 1_2:+56100651 None:intergenic
AGGAGAGATACCATATGCTT+GGG 0.509590 1_2:-56099034 Msa0078500:three_prime_UTR
AGAGTGGGTTGAGGGAAGGT+GGG 0.509779 1_2:+56100739 None:intergenic
TTCCTCTCAGAAGTTGGACC+AGG 0.511117 1_2:+56099610 None:intergenic
AATGTAGGATGATCCATGTA+TGG 0.511687 1_2:+56100674 None:intergenic
AATAATGGTACTATTGGATC+TGG 0.512155 1_2:-56099487 Msa0078500:three_prime_UTR
GGATAAGCGTTTATCAAGAA+AGG 0.513672 1_2:+56099987 None:intergenic
TTGTGGAAGGAAGGTAGGGT+TGG 0.515859 1_2:+56100264 None:intergenic
CAATAGCGGTGCCGGAATGT+AGG 0.516742 1_2:+56100659 None:intergenic
ACATGGATCATCCTACATTC+CGG 0.525681 1_2:-56100670 Msa0078500:CDS
GTTTCAACCAAACCAAGGAA+TGG 0.532105 1_2:-56099913 Msa0078500:CDS
GGCTGGCCTTCGAAAGGAGA+TGG 0.533326 1_2:-56099774 Msa0078500:CDS
TAGTTCGGAGATCAAACAAA+CGG 0.533677 1_2:-56097908 Msa0078500:three_prime_UTR
GACAATTTAGAGAAGAGTAA+AGG 0.534461 1_2:-56098913 Msa0078500:three_prime_UTR
CTCAAAGCTTTCGCCAACAC+GGG 0.535437 1_2:-56100374 Msa0078500:CDS
TGCATATTTCTGAAACAGGC+TGG 0.537703 1_2:-56099791 Msa0078500:CDS
TTATCCTTATAAGCAAAGTA+AGG 0.538288 1_2:+56099966 None:intergenic
CCAATCCTCAGATCCATACA+TGG 0.539084 1_2:-56100687 Msa0078500:exon
ATGTTGAATTGAATGTAGGT+AGG 0.540641 1_2:+56100774 None:intergenic
AAATAACCGGAGGAAGAAGT+TGG 0.548180 1_2:+56099445 None:intergenic
GTAACCCTAATAAAATGCAT+TGG 0.548583 1_2:-56100437 Msa0078500:CDS
TGTTGAATTGAATGTAGGTA+GGG 0.549256 1_2:+56100775 None:intergenic
TTCTCTTTAATGTCTCATGC+CGG 0.552007 1_2:-56098167 Msa0078500:three_prime_UTR
ACCCTAAACAAGCTCAAGCA+TGG 0.553134 1_2:-56100301 Msa0078500:CDS
GTGTTAAAGACTCAAACAAA+AGG 0.556735 1_2:+56097958 None:intergenic
TGTCTCATGCCGGTCTAGGA+AGG 0.561199 1_2:-56098157 Msa0078500:three_prime_UTR
TTTGTTTGAGTCTTTAACAC+CGG 0.562011 1_2:-56097955 Msa0078500:three_prime_UTR
ACGGGTATAGAATTCATGGT+AGG 0.563473 1_2:-56100356 Msa0078500:CDS
GAGGAAGAAGTTGGAGGATG+CGG 0.566760 1_2:+56099454 None:intergenic
ATCACCGTGCCATTAAATCT+GGG 0.568547 1_2:+56100835 None:intergenic
GAATCAATTGGAATAAACTA+CGG 0.568633 1_2:-56100497 Msa0078500:CDS
ACAGCATGCTATCATAATGC+AGG 0.570006 1_2:+56099874 None:intergenic
GTGAATCCAAAGAAGATACA+AGG 0.570723 1_2:-56098944 Msa0078500:three_prime_UTR
TGCACGTATGTGATAGTCGT+GGG 0.576145 1_2:+56097981 None:intergenic
AAAGGAGATGGAGATGAGGT+TGG 0.576671 1_2:-56099762 Msa0078500:CDS
ATGATCCATGTATGGATCTG+AGG 0.580676 1_2:+56100682 None:intergenic
AATGAGAGTGGGTTGAGGGA+AGG 0.581439 1_2:+56100735 None:intergenic
GTAAATAATCTGGCATGGCG+GGG 0.582624 1_2:+56098109 None:intergenic
CTTAAGATGGAAGCAATGCA+TGG 0.582736 1_2:+56100032 None:intergenic
AATAAGTAAATAATCTGGCA+TGG 0.583154 1_2:+56098104 None:intergenic
AGTAATGTTGAATTGAATGT+AGG 0.584117 1_2:+56100770 None:intergenic
CGAAAAGTTCCGGAAGAAGG+TGG 0.586411 1_2:+56100068 None:intergenic
CGGTAAATAAAACCTAGGTA+AGG 0.587112 1_2:-56097888 Msa0078500:three_prime_UTR
CATGAATTCTATACCCGTGT+TGG 0.588082 1_2:+56100361 None:intergenic
CGGGTATAGAATTCATGGTA+GGG 0.589149 1_2:-56100355 Msa0078500:CDS
GAAACAGGCTGGCCTTCGAA+AGG 0.591244 1_2:-56099780 Msa0078500:CDS
GAAGGAAATGTGGACATCAA+GGG 0.594648 1_2:-56098139 Msa0078500:three_prime_UTR
TCATCTCCATCTCCTTTCGA+AGG 0.594882 1_2:+56099768 None:intergenic
TCTCAAAGCTTTCGCCAACA+CGG 0.595063 1_2:-56100375 Msa0078500:CDS
GAAGAAGTTGGAGGATGCGG+CGG 0.595408 1_2:+56099457 None:intergenic
GTATGAAATGATCCTTACCT+AGG 0.597877 1_2:+56097876 None:intergenic
TTTAATGAGAATTTGAAACC+TGG 0.603078 1_2:-56099628 Msa0078500:exon
TAACCGGAGGAAGAAGTTGG+AGG 0.618276 1_2:+56099448 None:intergenic
CCTGTTTCAGAAATATGCAC+CGG 0.622309 1_2:+56099795 None:intergenic
GTTTGAGTCTTTAACACCGG+TGG 0.622658 1_2:-56097952 Msa0078500:three_prime_UTR
AAGTAAATAATCTGGCATGG+CGG 0.623238 1_2:+56098107 None:intergenic
TTATTACCACCATCATTCGA+AGG 0.630115 1_2:+56099505 None:intergenic
ACAAACGGTAAATAAAACCT+AGG 0.630500 1_2:-56097893 Msa0078500:three_prime_UTR
TTCGAAAGGAGATGGAGATG+AGG 0.634668 1_2:-56099766 Msa0078500:CDS
AATGGTACTATTGGATCTGG+TGG 0.638028 1_2:-56099484 Msa0078500:three_prime_UTR
ATTAGGTTACGATAAAATGC+CGG 0.638184 1_2:-56099814 Msa0078500:CDS
GTGCACGTATGTGATAGTCG+TGG 0.640982 1_2:+56097980 None:intergenic
GCCGGTCTAGGAAGGAAATG+TGG 0.641614 1_2:-56098149 Msa0078500:three_prime_UTR
AAATCAACTTCAGGTCTCAA+CGG 0.641716 1_2:+56099672 None:intergenic
TGAATCCAAAGAAGATACAA+GGG 0.657656 1_2:-56098943 Msa0078500:three_prime_UTR
AGTCCTCCTTCGAATGATGG+TGG 0.666041 1_2:-56099511 Msa0078500:three_prime_UTR
ATGACCCAGATTTAATGGCA+CGG 0.667459 1_2:-56100839 Msa0078500:five_prime_UTR
TTACCACCATCATTCGAAGG+AGG 0.670183 1_2:+56099508 None:intergenic
GAATCCAAAGAAGATACAAG+GGG 0.681565 1_2:-56098942 Msa0078500:three_prime_UTR
AAAAGGGAATGAATGAGAGT+GGG 0.687006 1_2:+56100724 None:intergenic
TGATGATGAAGTTGCAATAG+CGG 0.687297 1_2:+56100645 None:intergenic
AACCTGGTCCAACTTCTGAG+AGG 0.694615 1_2:-56099612 Msa0078500:three_prime_UTR
AGAAATGGCCAAATAACCGG+AGG 0.696188 1_2:+56099435 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTATTATTTTACTTTTA+GGG - chr1_2:56099531-56099550 Msa0078500:three_prime_UTR 10.0%
!! TAATAATTTAGTAAAAAAAC+AGG - chr1_2:56099582-56099601 Msa0078500:three_prime_UTR 10.0%
!! AATAATTTAGTAAAAAAACA+GGG - chr1_2:56099583-56099602 Msa0078500:three_prime_UTR 10.0%
!! ATATAATAATAAGATGTAGT+AGG + chr1_2:56098111-56098130 None:intergenic 15.0%
!!! CGTTTTATTATTTTACTTTT+AGG - chr1_2:56099530-56099549 Msa0078500:three_prime_UTR 15.0%
!! TATAAAATTTCGATGAATAT+TGG + chr1_2:56100181-56100200 None:intergenic 15.0%
!! CTAAAATAATGTATTATGTA+GGG + chr1_2:56100222-56100241 None:intergenic 15.0%
!! ACTAAAATAATGTATTATGT+AGG + chr1_2:56100223-56100242 None:intergenic 15.0%
!! ACATAAATAAGTAAATAATC+TGG + chr1_2:56100598-56100617 None:intergenic 15.0%
!! TAATATGATATGATTTGATA+TGG - chr1_2:56100663-56100682 Msa0078500:CDS 15.0%
!! TAAATATTCAAATCAACTTC+AGG + chr1_2:56099034-56099053 None:intergenic 20.0%
!! AACTTCAAAATCATCAATAA+TGG + chr1_2:56099707-56099726 None:intergenic 20.0%
!!! ATTTATGTATCCCATTTTAT+TGG - chr1_2:56100610-56100629 Msa0078500:intron 20.0%
!! AAAATGAGAACCAATAAAAT+GGG + chr1_2:56100623-56100642 None:intergenic 20.0%
!!! TAAAGAAAAGAGTTTTAGTT+CGG - chr1_2:56100771-56100790 Msa0078500:five_prime_UTR 20.0%
! AGTAATGTTGAATTGAATGT+AGG + chr1_2:56097927-56097946 None:intergenic 25.0%
!! ATCATTAGCATTTTCATTAC+AGG - chr1_2:56098071-56098090 Msa0078500:three_prime_UTR 25.0%
! GAATCAATTGGAATAAACTA+CGG - chr1_2:56098197-56098216 Msa0078500:intron 25.0%
!! AAAAATGGAAGTGATTTTTG+TGG + chr1_2:56098450-56098469 None:intergenic 25.0%
!!! AAAATCACTTCCATTTTTGT+AGG - chr1_2:56098452-56098471 Msa0078500:intron 25.0%
! AACTTCATTTCCTACAAAAA+TGG + chr1_2:56098465-56098484 None:intergenic 25.0%
! TTATCCTTACTTTGCTTATA+AGG - chr1_2:56098724-56098743 Msa0078500:intron 25.0%
! TTATCCTTATAAGCAAAGTA+AGG + chr1_2:56098731-56098750 None:intergenic 25.0%
! AATCTAACGATATTTGCTTA+GGG + chr1_2:56098754-56098773 None:intergenic 25.0%
! TAATCTAACGATATTTGCTT+AGG + chr1_2:56098755-56098774 None:intergenic 25.0%
!! AAAATGCGAGAAAGTATAAT+GGG - chr1_2:56098966-56098985 Msa0078500:three_prime_UTR 25.0%
! ATAAAGTTGAGTAGTAAGAA+AGG - chr1_2:56098995-56099014 Msa0078500:three_prime_UTR 25.0%
!! TTTAATGAGAATTTGAAACC+TGG - chr1_2:56099066-56099085 Msa0078500:intron 25.0%
! AAACGAGTTAATATCATAGT+GGG + chr1_2:56099515-56099534 None:intergenic 25.0%
! AAAACGAGTTAATATCATAG+TGG + chr1_2:56099516-56099535 None:intergenic 25.0%
! TCCTTGTTGAACTTAAAAAT+AGG + chr1_2:56099557-56099576 None:intergenic 25.0%
!!! TTGATGATTTTGAAGTTTTG+AGG - chr1_2:56099710-56099729 Msa0078500:CDS 25.0%
! TATAATAACTCAACTTCTGT+TGG + chr1_2:56099839-56099858 None:intergenic 25.0%
! GTTGAGTTATTATATTCATG+TGG - chr1_2:56099845-56099864 Msa0078500:CDS 25.0%
! TGAAATAATTCCAAAAGCAA+AGG + chr1_2:56099899-56099918 None:intergenic 25.0%
!! ATATCTACTCTAAAGATCAA+CGG + chr1_2:56100043-56100062 None:intergenic 25.0%
!! ATTTTTGTCATAAACTAAGC+TGG - chr1_2:56100260-56100279 Msa0078500:CDS 25.0%
! GTATTAATATTCGAAACCTA+TGG + chr1_2:56100360-56100379 None:intergenic 25.0%
! GCAAAAGGAAAACATAAAAA+GGG + chr1_2:56100486-56100505 None:intergenic 25.0%
! TGCAAAAGGAAAACATAAAA+AGG + chr1_2:56100487-56100506 None:intergenic 25.0%
!! TTTTTATGTTTTCCTTTTGC+AGG - chr1_2:56100486-56100505 Msa0078500:CDS 25.0%
! AATAAGTAAATAATCTGGCA+TGG + chr1_2:56100593-56100612 None:intergenic 25.0%
! CAAAATGAGAACCAATAAAA+TGG + chr1_2:56100624-56100643 None:intergenic 25.0%
TTTGTATGACCCAGATTTAA+TGG - chr1_2:56097850-56097869 Msa0078500:three_prime_UTR 30.0%
TGTTGAATTGAATGTAGGTA+GGG + chr1_2:56097922-56097941 None:intergenic 30.0%
ATGTTGAATTGAATGTAGGT+AGG + chr1_2:56097923-56097942 None:intergenic 30.0%
GCAACAAAATCTGAATCAAT+TGG - chr1_2:56098185-56098204 Msa0078500:three_prime_UTR 30.0%
GTAACCCTAATAAAATGCAT+TGG - chr1_2:56098257-56098276 Msa0078500:intron 30.0%
! TGCTCCAATGCATTTTATTA+GGG + chr1_2:56098264-56098283 None:intergenic 30.0%
! TTGCTCCAATGCATTTTATT+AGG + chr1_2:56098265-56098284 None:intergenic 30.0%
!! TTGTGACAGTGATTTGTTTA+TGG + chr1_2:56098571-56098590 None:intergenic 30.0%
TATATCAGAACGAAAAGTTC+CGG + chr1_2:56098639-56098658 None:intergenic 30.0%
TTTGGATTGAAAGCTTAAGA+TGG + chr1_2:56098678-56098697 None:intergenic 30.0%
TTAAGCTTTCAATCCAAAAC+TGG - chr1_2:56098680-56098699 Msa0078500:intron 30.0%
!! TCATAATGCAGGTTTGTTTT+TGG + chr1_2:56098812-56098831 None:intergenic 30.0%
ATTAGGTTACGATAAAATGC+CGG - chr1_2:56098880-56098899 Msa0078500:intron 30.0%
! GAAAATGCGAGAAAGTATAA+TGG - chr1_2:56098965-56098984 Msa0078500:three_prime_UTR 30.0%
GAGAATCCAAGATTATAAAC+AGG + chr1_2:56099138-56099157 None:intergenic 30.0%
AATAATGGTACTATTGGATC+TGG - chr1_2:56099207-56099226 Msa0078500:intron 30.0%
!!! GCCTATTTTTAAGTTCAACA+AGG - chr1_2:56099553-56099572 Msa0078500:three_prime_UTR 30.0%
! GTATAGTTTTTCTTTGACTC+AGG - chr1_2:56099640-56099659 Msa0078500:CDS 30.0%
ATAATGAAATCCCAAGCATA+TGG + chr1_2:56099673-56099692 None:intergenic 30.0%
CATCAATAATGGTAGAAGAA+AGG + chr1_2:56099696-56099715 None:intergenic 30.0%
TGAATCCAAAGAAGATACAA+GGG - chr1_2:56099751-56099770 Msa0078500:CDS 30.0%
GACAATTTAGAGAAGAGTAA+AGG - chr1_2:56099781-56099800 Msa0078500:CDS 30.0%
TTGTTACTAACTTTGTGAGT+TGG - chr1_2:56100139-56100158 Msa0078500:CDS 30.0%
ATCACACACTTTAATGTTGT+TGG - chr1_2:56100413-56100432 Msa0078500:CDS 30.0%
TCACACACTTTAATGTTGTT+GGG - chr1_2:56100414-56100433 Msa0078500:CDS 30.0%
GTGTTAAAGACTCAAACAAA+AGG + chr1_2:56100739-56100758 None:intergenic 30.0%
TTTGTTTGAGTCTTTAACAC+CGG - chr1_2:56100739-56100758 Msa0078500:five_prime_UTR 30.0%
ACAAACGGTAAATAAAACCT+AGG - chr1_2:56100801-56100820 Msa0078500:five_prime_UTR 30.0%
! CATACTCAGATTTTCTCAAA+CGG - chr1_2:56100836-56100855 Msa0078500:five_prime_UTR 30.0%
TAATGGCACGGTGATATTAA+TGG - chr1_2:56097867-56097886 Msa0078500:three_prime_UTR 35.0%
AAAAGGGAATGAATGAGAGT+GGG + chr1_2:56097973-56097992 None:intergenic 35.0%
GGGAATGTGAATAGTGAAAA+GGG + chr1_2:56097989-56098008 None:intergenic 35.0%
TGGGAATGTGAATAGTGAAA+AGG + chr1_2:56097990-56098009 None:intergenic 35.0%
AATGTAGGATGATCCATGTA+TGG + chr1_2:56098023-56098042 None:intergenic 35.0%
! TGATGATGAAGTTGCAATAG+CGG + chr1_2:56098052-56098071 None:intergenic 35.0%
! ATTAGGGTTACTACGTTTTC+TGG + chr1_2:56098248-56098267 None:intergenic 35.0%
! ATTTTTGTGGAAGGAAGGTA+GGG + chr1_2:56098437-56098456 None:intergenic 35.0%
! AAGTGATTTTTGTGGAAGGA+AGG + chr1_2:56098442-56098461 None:intergenic 35.0%
! ATGGAAGTGATTTTTGTGGA+AGG + chr1_2:56098446-56098465 None:intergenic 35.0%
GGATAAGCGTTTATCAAGAA+AGG + chr1_2:56098710-56098729 None:intergenic 35.0%
!!! GTTTTGTTTCAACCAAACCA+AGG - chr1_2:56098776-56098795 Msa0078500:intron 35.0%
! TTTTTGGATCAACCATTCCT+TGG + chr1_2:56098796-56098815 None:intergenic 35.0%
AAATCAACTTCAGGTCTCAA+CGG + chr1_2:56099025-56099044 None:intergenic 35.0%
! TATGGATTGTTTAAGCCTGA+TGG - chr1_2:56099108-56099127 Msa0078500:intron 35.0%
! ATGGATTGTTTAAGCCTGAT+GGG - chr1_2:56099109-56099128 Msa0078500:intron 35.0%
TTATAAACAGGATTCCCATC+AGG + chr1_2:56099126-56099145 None:intergenic 35.0%
GGGAATCCTGTTTATAATCT+TGG - chr1_2:56099129-56099148 Msa0078500:intron 35.0%
TTATTACCACCATCATTCGA+AGG + chr1_2:56099192-56099211 None:intergenic 35.0%
!! TCGAATGATGGTGGTAATAA+TGG - chr1_2:56099192-56099211 Msa0078500:intron 35.0%
GGTGGTAATAATGGTACTAT+TGG - chr1_2:56099201-56099220 Msa0078500:intron 35.0%
AACAGGGCTTAAGACAAAAA+TGG - chr1_2:56099599-56099618 Msa0078500:three_prime_UTR 35.0%
GTGAATCCAAAGAAGATACA+AGG - chr1_2:56099750-56099769 Msa0078500:CDS 35.0%
GAATCCAAAGAAGATACAAG+GGG - chr1_2:56099752-56099771 Msa0078500:CDS 35.0%
CATGATGAGAAAGGAGTAAA+AGG - chr1_2:56099804-56099823 Msa0078500:CDS 35.0%
GTTATTATATTCATGTGGCG+TGG - chr1_2:56099850-56099869 Msa0078500:CDS 35.0%
CATTGAGAAACGACACTTTA+TGG + chr1_2:56099983-56100002 None:intergenic 35.0%
!! CATAAAGTGTCGTTTCTCAA+TGG - chr1_2:56099981-56100000 Msa0078500:CDS 35.0%
ACTAAGCTGGTTGCAATAAT+TGG - chr1_2:56100273-56100292 Msa0078500:CDS 35.0%
CTAGCATTATTGTATCCGTT+AGG - chr1_2:56100379-56100398 Msa0078500:CDS 35.0%
TGATTCTGTCAGTTTCCTAA+CGG + chr1_2:56100397-56100416 None:intergenic 35.0%
GTATGTATTCTTCCTGCAAA+AGG + chr1_2:56100501-56100520 None:intergenic 35.0%
TTCTCTTTAATGTCTCATGC+CGG - chr1_2:56100527-56100546 Msa0078500:intron 35.0%
AAGTAAATAATCTGGCATGG+CGG + chr1_2:56100590-56100609 None:intergenic 35.0%
!!! CTTTTCTTTAGAACTACCAC+CGG + chr1_2:56100761-56100780 None:intergenic 35.0%
TAGTTCGGAGATCAAACAAA+CGG - chr1_2:56100786-56100805 Msa0078500:five_prime_UTR 35.0%
CGGTAAATAAAACCTAGGTA+AGG - chr1_2:56100806-56100825 Msa0078500:five_prime_UTR 35.0%
GTATGAAATGATCCTTACCT+AGG + chr1_2:56100821-56100840 None:intergenic 35.0%
ATGACCCAGATTTAATGGCA+CGG - chr1_2:56097855-56097874 Msa0078500:three_prime_UTR 40.0%
! ATCACCGTGCCATTAAATCT+GGG + chr1_2:56097862-56097881 None:intergenic 40.0%
! TATCACCGTGCCATTAAATC+TGG + chr1_2:56097863-56097882 None:intergenic 40.0%
!! AATGAATGAGAGTGGGTTGA+GGG + chr1_2:56097966-56097985 None:intergenic 40.0%
GAAAAGGGAATGAATGAGAG+TGG + chr1_2:56097974-56097993 None:intergenic 40.0%
CATGTATGGATCTGAGGATT+GGG + chr1_2:56098009-56098028 None:intergenic 40.0%
ATGATCCATGTATGGATCTG+AGG + chr1_2:56098015-56098034 None:intergenic 40.0%
ACATGGATCATCCTACATTC+CGG - chr1_2:56098024-56098043 Msa0078500:three_prime_UTR 40.0%
AAACAGTGTCCTATTTGTGC+AGG - chr1_2:56098158-56098177 Msa0078500:three_prime_UTR 40.0%
!! GTTACTACGTTTTCTGGTGA+TGG + chr1_2:56098242-56098261 None:intergenic 40.0%
!!! GCGAAAGCTTTGAGAACTTT+TGG + chr1_2:56098314-56098333 None:intergenic 40.0%
CATGAATTCTATACCCGTGT+TGG + chr1_2:56098336-56098355 None:intergenic 40.0%
CAACACGGGTATAGAATTCA+TGG - chr1_2:56098334-56098353 Msa0078500:intron 40.0%
ACGGGTATAGAATTCATGGT+AGG - chr1_2:56098338-56098357 Msa0078500:intron 40.0%
CGGGTATAGAATTCATGGTA+GGG - chr1_2:56098339-56098358 Msa0078500:intron 40.0%
ATAGAATTCATGGTAGGGCT+TGG - chr1_2:56098344-56098363 Msa0078500:intron 40.0%
! TCCATGCTTGAGCTTGTTTA+GGG + chr1_2:56098397-56098416 None:intergenic 40.0%
! ATCCATGCTTGAGCTTGTTT+AGG + chr1_2:56098398-56098417 None:intergenic 40.0%
! GATTTTTGTGGAAGGAAGGT+AGG + chr1_2:56098438-56098457 None:intergenic 40.0%
ACTTCTTATCCACCTTCTTC+CGG - chr1_2:56098617-56098636 Msa0078500:intron 40.0%
CTTAAGATGGAAGCAATGCA+TGG + chr1_2:56098665-56098684 None:intergenic 40.0%
! CAAGAAAGGTGAACCAGTTT+TGG + chr1_2:56098696-56098715 None:intergenic 40.0%
GTTTCAACCAAACCAAGGAA+TGG - chr1_2:56098781-56098800 Msa0078500:intron 40.0%
ACAGCATGCTATCATAATGC+AGG + chr1_2:56098823-56098842 None:intergenic 40.0%
CCTGTTTCAGAAATATGCAC+CGG + chr1_2:56098902-56098921 None:intergenic 40.0%
!! TGCATATTTCTGAAACAGGC+TGG - chr1_2:56098903-56098922 Msa0078500:three_prime_UTR 40.0%
CCATAATTCCTCTCAGAAGT+TGG + chr1_2:56099093-56099112 None:intergenic 40.0%
! CCAACTTCTGAGAGGAATTA+TGG - chr1_2:56099090-56099109 Msa0078500:intron 40.0%
AATGGTACTATTGGATCTGG+TGG - chr1_2:56099210-56099229 Msa0078500:intron 40.0%
AAATAACCGGAGGAAGAAGT+TGG + chr1_2:56099252-56099271 None:intergenic 40.0%
AACTTCTTCCTCCGGTTATT+TGG - chr1_2:56099251-56099270 Msa0078500:intron 40.0%
TCCAGAAATGGCCAAATAAC+CGG + chr1_2:56099265-56099284 None:intergenic 40.0%
!! CTATCATTTTGATCTTCCCC+CGG - chr1_2:56099412-56099431 Msa0078500:intron 40.0%
TTTACGAGACTCGTGTTATC+CGG + chr1_2:56099434-56099453 None:intergenic 40.0%
AGGAGAGATACCATATGCTT+GGG - chr1_2:56099660-56099679 Msa0078500:CDS 40.0%
! GAGTAAAGGCATGATGAGAA+AGG - chr1_2:56099795-56099814 Msa0078500:CDS 40.0%
! CTTTGCTTGTCCTTTGCTTT+TGG - chr1_2:56099886-56099905 Msa0078500:CDS 40.0%
GGCAAATTTCAGTTTCCAAG+AGG + chr1_2:56099962-56099981 None:intergenic 40.0%
AAGAGATTTCACGCTAGAAG+CGG - chr1_2:56100075-56100094 Msa0078500:CDS 40.0%
TGAGTTACCAGTGACAATCT+TGG - chr1_2:56100325-56100344 Msa0078500:CDS 40.0%
TTACCAGTGACAATCTTGGA+CGG - chr1_2:56100329-56100348 Msa0078500:CDS 40.0%
CACACTTTAATGTTGTTGGG+TGG - chr1_2:56100417-56100436 Msa0078500:CDS 40.0%
GAAGGAAATGTGGACATCAA+GGG - chr1_2:56100555-56100574 Msa0078500:intron 40.0%
AGTAAATAATCTGGCATGGC+GGG + chr1_2:56100589-56100608 None:intergenic 40.0%
! CTCAAACGGTTTTACTGATG+AGG - chr1_2:56100850-56100869 Msa0078500:five_prime_UTR 40.0%
!! GAATGAATGAGAGTGGGTTG+AGG + chr1_2:56097967-56097986 None:intergenic 45.0%
CCATGTATGGATCTGAGGAT+TGG + chr1_2:56098010-56098029 None:intergenic 45.0%
CCAATCCTCAGATCCATACA+TGG - chr1_2:56098007-56098026 Msa0078500:three_prime_UTR 45.0%
GTTGCTAGTCCTGCACAAAT+AGG + chr1_2:56098170-56098189 None:intergenic 45.0%
!!! CTACGTTTTCTGGTGATGGT+AGG + chr1_2:56098238-56098257 None:intergenic 45.0%
TCTCAAAGCTTTCGCCAACA+CGG - chr1_2:56098319-56098338 Msa0078500:intron 45.0%
ACCCTAAACAAGCTCAAGCA+TGG - chr1_2:56098393-56098412 Msa0078500:intron 45.0%
GTGTGAACACTTTGCATTGC+TGG + chr1_2:56098527-56098546 None:intergenic 45.0%
CACACTGCACTCATAGTTCT+TGG - chr1_2:56098542-56098561 Msa0078500:intron 45.0%
GAACGAAAAGTTCCGGAAGA+AGG + chr1_2:56098632-56098651 None:intergenic 45.0%
GGATCAACCATTCCTTGGTT+TGG + chr1_2:56098791-56098810 None:intergenic 45.0%
! TATTCTGCGCTTGGAGCATT+AGG - chr1_2:56098863-56098882 Msa0078500:intron 45.0%
!! CCGGTGCATATTTCTGAAAC+AGG - chr1_2:56098899-56098918 Msa0078500:three_prime_UTR 45.0%
TCATCTCCATCTCCTTTCGA+AGG + chr1_2:56098929-56098948 None:intergenic 45.0%
TTCGAAAGGAGATGGAGATG+AGG - chr1_2:56098928-56098947 Msa0078500:three_prime_UTR 45.0%
AAAGGAGATGGAGATGAGGT+TGG - chr1_2:56098932-56098951 Msa0078500:three_prime_UTR 45.0%
TTACCACCATCATTCGAAGG+AGG + chr1_2:56099189-56099208 None:intergenic 45.0%
AGAAATGGCCAAATAACCGG+AGG + chr1_2:56099262-56099281 None:intergenic 45.0%
TCCGGTTATTTGGCCATTTC+TGG - chr1_2:56099261-56099280 Msa0078500:intron 45.0%
CAGTGAAGTAGCTCCAGAAA+TGG + chr1_2:56099277-56099296 None:intergenic 45.0%
!! CATTTCTGGAGCTACTTCAC+TGG - chr1_2:56099275-56099294 Msa0078500:intron 45.0%
TTACGAGACTCGTGTTATCC+GGG + chr1_2:56099433-56099452 None:intergenic 45.0%
CAGGAGAGATACCATATGCT+TGG - chr1_2:56099659-56099678 Msa0078500:CDS 45.0%
GATGCCCCTTGTATCTTCTT+TGG + chr1_2:56099759-56099778 None:intergenic 45.0%
CAGTTTCCAAGAGGAGACAA+CGG + chr1_2:56099953-56099972 None:intergenic 45.0%
AACGGTGTACTACTCTCTAG+TGG + chr1_2:56100025-56100044 None:intergenic 45.0%
GTGGCACATGTTATGCTAGT+TGG - chr1_2:56100436-56100455 Msa0078500:CDS 45.0%
TTAATGTCTCATGCCGGTCT+AGG - chr1_2:56100533-56100552 Msa0078500:intron 45.0%
TCCACATTTCCTTCCTAGAC+CGG + chr1_2:56100549-56100568 None:intergenic 45.0%
GGAAGGAAATGTGGACATCA+AGG - chr1_2:56100554-56100573 Msa0078500:intron 45.0%
GTAAATAATCTGGCATGGCG+GGG + chr1_2:56100588-56100607 None:intergenic 45.0%
TGCACGTATGTGATAGTCGT+GGG + chr1_2:56100716-56100735 None:intergenic 45.0%
GTTTGAGTCTTTAACACCGG+TGG - chr1_2:56100742-56100761 Msa0078500:five_prime_UTR 45.0%
!! AATGAGAGTGGGTTGAGGGA+AGG + chr1_2:56097962-56097981 None:intergenic 50.0%
TGAAGTTGCAATAGCGGTGC+CGG + chr1_2:56098046-56098065 None:intergenic 50.0%
CTCAAAGCTTTCGCCAACAC+GGG - chr1_2:56098320-56098339 Msa0078500:intron 50.0%
TTGTGGAAGGAAGGTAGGGT+TGG + chr1_2:56098433-56098452 None:intergenic 50.0%
CGAAAAGTTCCGGAAGAAGG+TGG + chr1_2:56098629-56098648 None:intergenic 50.0%
GACGCTGTTTATTCTGCGCT+TGG - chr1_2:56098854-56098873 Msa0078500:intron 50.0%
AACCTGGTCCAACTTCTGAG+AGG - chr1_2:56099082-56099101 Msa0078500:intron 50.0%
TTCCTCTCAGAAGTTGGACC+AGG + chr1_2:56099087-56099106 None:intergenic 50.0%
TCGAGTCCTCCTTCGAATGA+TGG - chr1_2:56099180-56099199 Msa0078500:intron 50.0%
AGTCCTCCTTCGAATGATGG+TGG - chr1_2:56099183-56099202 Msa0078500:intron 50.0%
GAGGAAGAAGTTGGAGGATG+CGG + chr1_2:56099243-56099262 None:intergenic 50.0%
CATCCTCCAACTTCTTCCTC+CGG - chr1_2:56099243-56099262 Msa0078500:intron 50.0%
TAACCGGAGGAAGAAGTTGG+AGG + chr1_2:56099249-56099268 None:intergenic 50.0%
TACGAGACTCGTGTTATCCG+GGG + chr1_2:56099432-56099451 None:intergenic 50.0%
AACGTACCGTTGTCTCCTCT+TGG - chr1_2:56099944-56099963 Msa0078500:CDS 50.0%
CAACCGTCCAAGATTGTCAC+TGG + chr1_2:56100335-56100354 None:intergenic 50.0%
CAGTGACAATCTTGGACGGT+TGG - chr1_2:56100333-56100352 Msa0078500:CDS 50.0%
ATCTTGGACGGTTGGACCAT+AGG - chr1_2:56100341-56100360 Msa0078500:CDS 50.0%
GTGCACGTATGTGATAGTCG+TGG + chr1_2:56100717-56100736 None:intergenic 50.0%
!! AGAGTGGGTTGAGGGAAGGT+GGG + chr1_2:56097958-56097977 None:intergenic 55.0%
! CAATAGCGGTGCCGGAATGT+AGG + chr1_2:56098038-56098057 None:intergenic 55.0%
!! GAAACAGGCTGGCCTTCGAA+AGG - chr1_2:56098914-56098933 Msa0078500:three_prime_UTR 55.0%
GAAGAAGTTGGAGGATGCGG+CGG + chr1_2:56099240-56099259 None:intergenic 55.0%
ACGAGACTCGTGTTATCCGG+GGG + chr1_2:56099431-56099450 None:intergenic 55.0%
TGTCTCATGCCGGTCTAGGA+AGG - chr1_2:56100537-56100556 Msa0078500:intron 55.0%
GCCGGTCTAGGAAGGAAATG+TGG - chr1_2:56100545-56100564 Msa0078500:intron 55.0%
!! GAGAGTGGGTTGAGGGAAGG+TGG + chr1_2:56097959-56097978 None:intergenic 60.0%
!! GGCTGGCCTTCGAAAGGAGA+TGG - chr1_2:56098920-56098939 Msa0078500:three_prime_UTR 60.0%
! ATCTGGCATGGCGGGGCACA+AGG + chr1_2:56100581-56100600 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 56097841 56100875 56097841 ID=Msa0078500;Name=Msa0078500
chr1_2 mRNA 56097841 56100875 56097841 ID=Msa0078500-mRNA-1;Parent=Msa0078500;Name=Msa0078500-mRNA-1;_AED=0.15;_eAED=0.15;_QI=185|1|1|1|0.33|0.5|4|747|322
chr1_2 exon 56097841 56098208 56097841 ID=Msa0078500-mRNA-1:exon:17519;Parent=Msa0078500-mRNA-1
chr1_2 exon 56098891 56099054 56098891 ID=Msa0078500-mRNA-1:exon:17518;Parent=Msa0078500-mRNA-1
chr1_2 exon 56099420 56100536 56099420 ID=Msa0078500-mRNA-1:exon:17517;Parent=Msa0078500-mRNA-1
chr1_2 exon 56100624 56100875 56100624 ID=Msa0078500-mRNA-1:exon:17516;Parent=Msa0078500-mRNA-1
chr1_2 five_prime_UTR 56100691 56100875 56100691 ID=Msa0078500-mRNA-1:five_prime_utr;Parent=Msa0078500-mRNA-1
chr1_2 CDS 56100624 56100690 56100624 ID=Msa0078500-mRNA-1:cds;Parent=Msa0078500-mRNA-1
chr1_2 CDS 56099635 56100536 56099635 ID=Msa0078500-mRNA-1:cds;Parent=Msa0078500-mRNA-1
chr1_2 three_prime_UTR 56099420 56099634 56099420 ID=Msa0078500-mRNA-1:three_prime_utr;Parent=Msa0078500-mRNA-1
chr1_2 three_prime_UTR 56098891 56099054 56098891 ID=Msa0078500-mRNA-1:three_prime_utr;Parent=Msa0078500-mRNA-1
chr1_2 three_prime_UTR 56097841 56098208 56097841 ID=Msa0078500-mRNA-1:three_prime_utr;Parent=Msa0078500-mRNA-1
Gene Sequence

>Msa0078500

ATGGATCATCCTACATTCCGGCACCGCTATTGCAACTTCATCATCATCATTAGCATTTTCATTACAGGACTAGCAACAAAATCTGAATCAATTGGAATAAACTACGGTCAAATAGCAAACAACCTACCATCACCAGAAAACGTAGTAACCCTAATAAAATGCATTGGAGCAACAAAAGTAAAACTCTACGACGCAGATCCAAAAGTTCTCAAAGCTTTCGCCAACACGGGTATAGAATTCATGGTAGGGCTTGGAAATGAATATCTCTCTAAAATGAAAGACCCTAAACAAGCTCAAGCATGGATCAAAACAAATCTCCAACCCTACCTTCCTTCCACAAAAATCACTTCCATTTTTGTAGGAAATGAAGTTCTCACTTTCAATGACTCTTCTTTAACATCTTCTCTTCTACCAGCAATGCAAAGTGTTCACACTGCACTCATAGTTCTTGGTCTCCATAAACAAATCACTGTCACAACAACTCATTCATTAGCTATACTACAAACTTCTTATCCACCTTCTTCCGGAACTTTTCGTTCTGATATAGCTCCATGCATTGCTTCCATCTTAAGCTTTCAATCCAAAACTGGTTCACCTTTCTTGATAAACGCTTATCCTTACTTTGCTTATAAGGATAACCCTAAGCAAATATCGTTAGATTATGTTTTGTTTCAACCAAACCAAGGAATGGTTGATCCAAAAACAAACCTGCATTATGATAGCATGCTGTTTGCGCAGATCGACGCTGTTTATTCTGCGCTTGGAGCATTAGGTTACGATAAAATGCCGGTGCATATTTCTGAAACAGGCTGGCCTTCGAAAGGAGATGGAGATGAGGTTGGTGCTAATGTTGAAAATGCGAGAAAGTATAATGGGAATGTGATAAAGTTGAGTAGTAAGAAAGGAACACCGTTGAGACCTGAAGTTGATTTGAATATTTATGTTTTTGCACTTTTAATGAGAATTTGA
Protein sequence

>Msa0078500

MDHPTFRHRYCNFIIIISIFITGLATKSESIGINYGQIANNLPSPENVVTLIKCIGATKVKLYDADPKVLKAFANTGIEFMVGLGNEYLSKMKDPKQAQAWIKTNLQPYLPSTKITSIFVGNEVLTFNDSSLTSSLLPAMQSVHTALIVLGLHKQITVTTTHSLAILQTSYPPSSGTFRSDIAPCIASILSFQSKTGSPFLINAYPYFAYKDNPKQISLDYVLFQPNQGMVDPKTNLHYDSMLFAQIDAVYSALGALGYDKMPVHISETGWPSKGDGDEVGANVENARKYNGNVIKLSSKKGTPLRPEVDLNIYVFALLMRI*