AlfalfaGEDB | Msa1410160

M. sativa cultivar zhongmu-4 / Msa1410160

KEGG: -
GO: GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0016043 GO:0016049 GO:0022414 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0044706 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0060560 GO:0071840
PFAM: PF00651.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410160	sp\|Q9SA69\|Y1301_ARATH	68.837	645	174	9	1	629	1	634	0.0	862

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410160	A0A396HDB7	99.046	629	6	0	1	629	1	629	0.0	1284

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003080	Msa1410160	0.843761	1.148555e-58	-8.615850e-47
Msa0014770	Msa1410160	0.831434	1.673423e-55	-8.615850e-47
Msa0025160	Msa1410160	0.869142	3.863921e-66	-8.615850e-47
Msa0034210	Msa1410160	0.811508	6.760186e-51	-8.615850e-47
Msa0035380	Msa1410160	0.812788	3.553893e-51	-8.615850e-47
Msa0038530	Msa1410160	0.842180	3.025900e-58	-8.615850e-47
Msa0050660	Msa1410160	0.849125	3.951879e-60	-8.615850e-47
Msa0050700	Msa1410160	0.851995	6.170862e-61	-8.615850e-47
Msa0050790	Msa1410160	0.850031	2.207689e-60	-8.615850e-47
Msa0072520	Msa1410160	0.866574	2.580781e-65	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410160	MtrunA17_Chr7g0268781	99.046	629	6	0	1	629	1	629	0.0	1284
Msa1410160	MtrunA17_Chr1g0195911	76.899	632	132	6	1	629	1	621	0.0	998
Msa1410160	MtrunA17_Chr4g0010091	47.902	572	275	9	30	594	28	583	3.19e-179	521
Msa1410160	MtrunA17_Chr7g0245201	44.034	595	289	11	34	615	59	622	9.83e-163	480
Msa1410160	MtrunA17_Chr5g0401451	40.156	640	317	14	22	629	25	630	2.70e-151	451
Msa1410160	MtrunA17_Chr4g0053841	38.792	629	327	17	25	629	1	595	1.18e-136	412
Msa1410160	MtrunA17_Chr2g0303541	37.086	604	333	11	26	601	15	599	5.21e-123	378
Msa1410160	MtrunA17_Chr3g0108331	35.057	619	319	15	20	617	7	563	6.98e-117	360
Msa1410160	MtrunA17_Chr2g0279481	31.373	663	384	12	13	619	20	667	9.30e-108	340
Msa1410160	MtrunA17_Chr8g0374011	35.392	599	312	12	38	575	22	606	3.60e-107	337

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410160	AT1G03010.1	68.837	645	174	9	1	629	1	634	0.0	862
Msa1410160	AT1G03010.2	69.055	614	163	9	32	629	5	607	0.0	816
Msa1410160	AT1G03010.3	69.055	614	163	9	32	629	31	633	0.0	815
Msa1410160	AT2G47860.1	61.231	650	216	12	1	629	1	635	0.0	743
Msa1410160	AT2G47860.3	59.138	673	216	13	1	629	1	658	0.0	730
Msa1410160	AT2G47860.5	61.205	531	170	11	121	629	1	517	0.0	583
Msa1410160	AT2G47860.2	61.205	531	170	11	121	629	1	517	0.0	583
Msa1410160	AT2G47860.4	61.269	457	155	7	1	448	1	444	0.0	531
Msa1410160	AT2G47860.6	58.333	480	155	8	1	448	1	467	4.25e-180	518
Msa1410160	AT5G48800.1	46.540	578	278	8	23	594	28	580	1.90e-174	509

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Find 121 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGGAGTTGTAACTCT+TGG	0.524005	tig0023619:-27373	Msa1410160:CDS
AAAATTATAATACCATTCAG+TGG	0.645788	tig0023619:+27288	None:intergenic
AAACACATCGAACATTATAC+AGG	0.516060	tig0023619:-25474	Msa1410160:CDS
AAACATGAAAGAAATGCTAT+TGG	0.398126	tig0023619:+25032	None:intergenic
AAAGCAAAGCTCAATTCTTA+AGG	0.417656	tig0023619:-24736	Msa1410160:CDS
AAAGGTGGTGAATGTGTGTG+TGG	0.678039	tig0023619:+27627	None:intergenic
AACCAGAAACACCCTTAGAT+TGG	0.511015	tig0023619:-25292	Msa1410160:CDS
AACTTCTTGTAGAAGTGATT+TGG	0.280903	tig0023619:-24979	Msa1410160:CDS
AACTTTCCATTGACAGAAAC+TGG	0.249645	tig0023619:-27472	Msa1410160:five_prime_UTR
AAGAGCTGAATCATATCTAA+AGG	0.501938	tig0023619:-25510	Msa1410160:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCATGTCATTGTTTGGTAA+AGG	+	tig0023619:24402-24421	None:intergenic	30.0%
TTTCATATAGCTAACAGATG+AGG	+	tig0023619:24447-24466	None:intergenic	30.0%
CCAAAAAAATTGTCCATTGT+CGG	+	tig0023619:24553-24572	None:intergenic	30.0%
ATGGTGCAATAATAAGACAT+TGG	+	tig0023619:24738-24757	None:intergenic	30.0%
GACATGAAAATTTCAAAGCA+TGG	+	tig0023619:24855-24874	None:intergenic	30.0%
CTACGTGTATATAATGATTC+AGG	-	tig0023619:24874-24893	Msa1410160:CDS	30.0%
ATTTACCTTGTGAAGTGAAT+AGG	+	tig0023619:24968-24987	None:intergenic	30.0%
TGATACACCGTTAGTATAAA+AGG	-	tig0023619:25152-25171	Msa1410160:CDS	30.0%
ATAGCAAAACTACATTTGAC+AGG	+	tig0023619:25364-25383	None:intergenic	30.0%
AAGAGCTGAATCATATCTAA+AGG	-	tig0023619:25727-25746	Msa1410160:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0023619	gene	23559	27700	23559	ID=Msa1410160;Name=Msa1410160
tig0023619	mRNA	23559	27700	23559	ID=Msa1410160-mRNA-1;Parent=Msa1410160;Name=Msa1410160-mRNA-1;_AED=0.17;_eAED=0.17;_QI=308\|1\|1\|1\|1\|1\|4\|155\|629
tig0023619	exon	23559	24262	23559	ID=Msa1410160-mRNA-1:exon:26438;Parent=Msa1410160-mRNA-1
tig0023619	exon	24587	25768	24587	ID=Msa1410160-mRNA-1:exon:26437;Parent=Msa1410160-mRNA-1
tig0023619	exon	26278	26344	26278	ID=Msa1410160-mRNA-1:exon:26436;Parent=Msa1410160-mRNA-1
tig0023619	exon	27301	27700	27301	ID=Msa1410160-mRNA-1:exon:26435;Parent=Msa1410160-mRNA-1
tig0023619	five_prime_UTR	27393	27700	27393	ID=Msa1410160-mRNA-1:five_prime_utr;Parent=Msa1410160-mRNA-1
tig0023619	CDS	27301	27392	27301	ID=Msa1410160-mRNA-1:cds;Parent=Msa1410160-mRNA-1
tig0023619	CDS	26278	26344	26278	ID=Msa1410160-mRNA-1:cds;Parent=Msa1410160-mRNA-1
tig0023619	CDS	24587	25768	24587	ID=Msa1410160-mRNA-1:cds;Parent=Msa1410160-mRNA-1
tig0023619	CDS	23714	24262	23714	ID=Msa1410160-mRNA-1:cds;Parent=Msa1410160-mRNA-1
tig0023619	three_prime_UTR	23559	23713	23559	ID=Msa1410160-mRNA-1:three_prime_utr;Parent=Msa1410160-mRNA-1

Gene Sequence

>Msa1410160

ATGGGAGTTGTAACTCTTGGTGAATTGAAGCCTAGTGTTTCAGGAAGAAGGACTTTTCGTCCAAGTTCAAGCATAAGACATGCCACTGAATGGCCAATATCTGATGTCTCCAGTGATCTTACCATCCAAGTAGGAGCATCAACCTATTCACTTCACAAGTTTCCTCTTGTTTCTAGGAGTGGAAGATTTAGGAAGCTGCTATCTGAATCAAAAGATTCTAAAGTTCCGCGCGTAAGTCTTCCGACCGTGCCAGGTGGTGTAGATGCATTTGAAATAGCTGCAAAATTCTGCTATGGAATCAATGTGGAATTCACCCTCTCGAATATTGCTATGCTATATTGTATATCTCATTTTCTTGAAATGACAGAAGAATTCTCAGAAAAGAATTTGGTGACAAGAGCTGAATCATATCTAAAGGAAACGGTACTCCCAAACACATCGAACATTATACAGGTTCTTCACCATTGTGAAAATCTTGTTCCTTTATCAGAAGATATCAACCTTGTTAATAGGCTAATCAATGCAATTGCAAATAATGCATGCAAAGAGCAACTAACTTCTGGTTTGTTAAAACTTGATCACAACTTTCCTTCTAAAACATTATCAATGATGGAACCAGAAACACCCTTAGATTGGTGGGGAAACTCTTTCAACGTTCTTAGTCTTGATTTCTTCGAACGAGTTATATCGGTTATGAAATCGAAGGGATTAACACAAGAGTTGATCAGCAAAATTTTGGTAAACTATGCACATAATTCTCTTCAAGGGACTAGTAGTGTTAGTTCCCTTGACATTGAATTGCAGAAGAAACAAAGGGTCATTGTTGAAACTATAGTTCGCTTACTACCAACTCAATCTAGGAAAAGCTCAGTTCCAATAGCATTTCTTTCATGTTTATTGAAAACTGCAATAGCAACATTAGTATCAACTTCTTGTAGAAGTGATTTGGAGAGGCGGATCGGTTTACAACTCGATCAGGCAATTCTTGAAGACATACTTATTCCAACGAATTCACCTTCGAGTGCTCACAACACAATGTATGATATAGATTCAATTTTGAGGATCTTTTCTACTTTTCTGAATTTGGACGAGGTGGATGATGAAGACAGTGACTTCAGAGATGAAAATCAAATGATGTATGAGTATGAATTTGACAGTCCTGGATCTCCAAAGCAAAGCTCAATTCTTAAGGTATCCAAATTACTTGACAATTTTCTCGCTGAAGTAGCACTAGACTCAAACTTGTTGCCATCAAAATTCATAGCACTTGCAGAACTACTTCCGGATCATGCTCGCGTTACAAGCGATGGACTCTATCGAGCTGTTGACATCTTCCTTAAGGTTCATCCGAACATTAAGGACTCGGAGCGATACCGCCTAAGCAAAACAATTGATTGTCAGAAACTATCTCAAGAAGCATGCAGCCATGCCGCACAAAATGAAAGGCTACCAGTTCAAATAGGTGTACAAGTTCTTTACTACGAACAAATAAGGCTTCGAAATGCGATGAATGGAGGACACAATCAAATCTTGTTTGGTGGTATTCATGGTCAATTTCCTCAAAGATCAGGGAGTGGTATAGGAAGTGGAGCAATTTCTCCTAGAGATAACTATGCATCGGTAAGAAGAGAGAATCGAGAGCTGAAGCTTGAAGTAACAAGAATGAGAATGAGGTTGAATGATTTGGAGAAAGATCATGTTTCTATGAAGCAAGAGCTTGTGAAGACACATCCTGCACGCAAATTCTTCAAATCATTTGCTCAAAAATTAAGAAAGTTCAATGCTCTATTCCGTATTAACAGTATTCGTGGCAGTGGAACTGGAAGGTCTAGTCCACAACCACGGTCCCGGTTTCCTTTTCAAAAGAGAAGGTGCCACTCAGTTTCATGA

Protein sequence

>Msa1410160

MGVVTLGELKPSVSGRRTFRPSSSIRHATEWPISDVSSDLTIQVGASTYSLHKFPLVSRSGRFRKLLSESKDSKVPRVSLPTVPGGVDAFEIAAKFCYGINVEFTLSNIAMLYCISHFLEMTEEFSEKNLVTRAESYLKETVLPNTSNIIQVLHHCENLVPLSEDINLVNRLINAIANNACKEQLTSGLLKLDHNFPSKTLSMMEPETPLDWWGNSFNVLSLDFFERVISVMKSKGLTQELISKILVNYAHNSLQGTSSVSSLDIELQKKQRVIVETIVRLLPTQSRKSSVPIAFLSCLLKTAIATLVSTSCRSDLERRIGLQLDQAILEDILIPTNSPSSAHNTMYDIDSILRIFSTFLNLDEVDDEDSDFRDENQMMYEYEFDSPGSPKQSSILKVSKLLDNFLAEVALDSNLLPSKFIALAELLPDHARVTSDGLYRAVDIFLKVHPNIKDSERYRLSKTIDCQKLSQEACSHAAQNERLPVQIGVQVLYYEQIRLRNAMNGGHNQILFGGIHGQFPQRSGSGIGSGAISPRDNYASVRRENRELKLEVTRMRMRLNDLEKDHVSMKQELVKTHPARKFFKSFAQKLRKFNALFRINSIRGSGTGRSSPQPRSRFPFQKRRCHSVS*