AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1384760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1384760 MtrunA17_Chr1g0154751 100.000 213 0 0 1 213 1 213 1.87e-159 439
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1384760 AT1G45170.1 69.484 213 64 1 1 213 2 213 8.28e-111 316
Msa1384760 AT5G42960.1 68.075 213 66 2 1 213 3 213 5.09e-109 311
Msa1384760 AT1G45170.2 55.399 213 48 2 1 213 2 167 1.92e-76 228

Find 97 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTGATTGCCTATAAAT+TGG 0.165071 tig0020679:-33455 Msa1384760:three_prime_UTR
AGCGGGGTTGGTGGTGAAAA+TGG 0.203881 tig0020679:+36521 None:intergenic
GCGAATTATGGTGTTGAATC+TGG 0.218241 tig0020679:-36065 Msa1384760:CDS
AGGGGATAACAGAACAATTT+TGG 0.234866 tig0020679:-36126 Msa1384760:CDS
AATTATAGGATTCTGCAATA+TGG 0.241988 tig0020679:+33472 None:intergenic
ATAAGGTTTGATATCTAGTT+TGG 0.262212 tig0020679:-33643 Msa1384760:three_prime_UTR
ATTTATAGGCAATCAATTAT+AGG 0.279411 tig0020679:+33458 None:intergenic
GAATAAAGTGCCCAATTTAT+AGG 0.281680 tig0020679:+33444 None:intergenic
AATAAGGTTTCAGCGAATTA+TGG 0.297969 tig0020679:-36077 Msa1384760:CDS
GGCTCCTCTGTGTTGCTGTT+GGG 0.309390 tig0020679:+33317 None:intergenic
CAATTGTAGTCAAACCCTTA+TGG 0.311402 tig0020679:+36015 None:intergenic
TTCAATTTATGAACACAGTT+AGG 0.317886 tig0020679:-36190 Msa1384760:CDS
GGCGACGGAGGCTGCGGCGG+CGG 0.338930 tig0020679:+36443 None:intergenic
AACAATGAAAGCCGCTGTTA+AGG 0.348424 tig0020679:-36490 Msa1384760:exon
GCTGTTTCGAGGAAAGTTTA+TGG 0.364076 tig0020679:-35954 Msa1384760:CDS
CTTCTCCTCGGCCAAGTTAA+CGG 0.367147 tig0020679:+33719 None:intergenic
TTCAAAATTCCATGTGGTCT+CGG 0.373502 tig0020679:+33677 None:intergenic
ACAATGAAAGCCGCTGTTAA+GGG 0.378379 tig0020679:-36489 Msa1384760:CDS
TGTGTGGGACCCATCGAATA+AGG 0.383360 tig0020679:-36093 Msa1384760:CDS
CGACTACAACGTCCCCAAAA+AGG 0.387317 tig0020679:-36307 Msa1384760:intron
GAGGGATGCATGAATAATAT+TGG 0.392922 tig0020679:+33388 None:intergenic
CAGACCACGAGTAAGAAGTT+AGG 0.393154 tig0020679:-35906 Msa1384760:CDS
ATTAACGGCCCAAGCTTAAC+CGG 0.393393 tig0020679:-36369 Msa1384760:CDS
ACAGACGCTACTTTCATTAA+CGG 0.400228 tig0020679:-36384 Msa1384760:CDS
GCCTCCGTCGCCTTCAACGC+CGG 0.402791 tig0020679:-36432 Msa1384760:CDS
TAAGGTTTGATATCTAGTTT+GGG 0.410066 tig0020679:-33642 Msa1384760:three_prime_UTR
GGTTGTGGCGTCCGTTAACT+TGG 0.413701 tig0020679:-33730 Msa1384760:CDS
TAATTGATTGCCTATAAATT+GGG 0.414424 tig0020679:-33454 Msa1384760:three_prime_UTR
TTTGACATCGCCGGCGTTGA+AGG 0.416806 tig0020679:+36422 None:intergenic
CGGCTCCTCTGTGTTGCTGT+TGG 0.426162 tig0020679:+33316 None:intergenic
ATTATAGGATTCTGCAATAT+GGG 0.432310 tig0020679:+33473 None:intergenic
TAACCTACATACACAGCAAA+GGG 0.443140 tig0020679:-36145 Msa1384760:CDS
TGAGTTCCTGCTGTTTGCGC+GGG 0.443439 tig0020679:+33266 None:intergenic
TGTGGTCTCGGCACTAAGCT+TGG 0.445547 tig0020679:+33689 None:intergenic
GAAGTATAGCTATGTCCATA+AGG 0.446086 tig0020679:-36030 Msa1384760:CDS
GCGGGGTTGGTGGTGAAAAT+GGG 0.460597 tig0020679:+36522 None:intergenic
TCAATTTATGAACACAGTTA+GGG 0.463428 tig0020679:-36189 Msa1384760:CDS
TCAAGTATACAATAATGAAA+TGG 0.464664 tig0020679:+33586 None:intergenic
GTGGTCTCGGCACTAAGCTT+GGG 0.465342 tig0020679:+33690 None:intergenic
AGGACTAAGCCGGTTAAGCT+TGG 0.467012 tig0020679:+36360 None:intergenic
GTAGTCGATACTGAAGGAAC+CGG 0.468560 tig0020679:+36323 None:intergenic
AAGTATAGCTATGTCCATAA+GGG 0.470434 tig0020679:-36029 Msa1384760:CDS
GGACTAAGCCGGTTAAGCTT+GGG 0.473049 tig0020679:+36361 None:intergenic
TGCTGTTTGCGCGGGCCTGT+AGG 0.474058 tig0020679:+33274 None:intergenic
TTCATTGTTAATGACGGAGC+GGG 0.474602 tig0020679:+36504 None:intergenic
CGAATTATGGTGTTGAATCT+GGG 0.476174 tig0020679:-36064 Msa1384760:CDS
TACAGGCCCGCGCAAACAGC+AGG 0.477584 tig0020679:-33272 Msa1384760:three_prime_UTR
AGTTAGGGTTTGAGTGGTCG+AGG 0.482159 tig0020679:-35890 Msa1384760:CDS
ATCAAATGAAAAGTTTAAAG+TGG 0.483426 tig0020679:+33519 None:intergenic
CTTGCAAGTATTGATTGACT+TGG 0.493911 tig0020679:+33160 None:intergenic
TGTTAATGACGGAGCGGGGT+TGG 0.495479 tig0020679:+36509 None:intergenic
ATCCCCTTTGCTGTGTATGT+AGG 0.496851 tig0020679:+36142 None:intergenic
GCTCCTCTGTGTTGCTGTTG+GGG 0.497041 tig0020679:+33318 None:intergenic
AGACCACGAGTAAGAAGTTA+GGG 0.497058 tig0020679:-35905 Msa1384760:CDS
TTCGCTGAAACCTTATTCGA+TGG 0.499631 tig0020679:+36083 None:intergenic
GTTGAAGGCGACGGAGGCTG+CGG 0.500163 tig0020679:+36437 None:intergenic
GTCGAGGAATTCGAAACAAA+GGG 0.505059 tig0020679:-35874 Msa1384760:CDS
ATCCCTCCTCATAAGAAAAG+AGG 0.512029 tig0020679:-33372 Msa1384760:three_prime_UTR
TTCTACGGCGAGGACTAAGC+CGG 0.514419 tig0020679:+36350 None:intergenic
TAATGACGGAGCGGGGTTGG+TGG 0.516072 tig0020679:+36512 None:intergenic
TTATGGTGATGACTCATTGA+AGG 0.523909 tig0020679:-35937 Msa1384760:CDS
TACAGAATAACTTCATGAGA+AGG 0.528811 tig0020679:+33216 None:intergenic
GAAGGCGACGGAGGCTGCGG+CGG 0.533980 tig0020679:+36440 None:intergenic
TGCTCGGAGTTTGACATCGC+CGG 0.539580 tig0020679:+36413 None:intergenic
GGCGTCCGTTAACTTGGCCG+AGG 0.541591 tig0020679:-33724 Msa1384760:CDS
TGTATGTAGGTTAAATTCAA+CGG 0.544412 tig0020679:+36155 None:intergenic
AGCTTAGTGCCGAGACCACA+TGG 0.549160 tig0020679:-33686 Msa1384760:CDS
TTGCAAGTATTGATTGACTT+GGG 0.550118 tig0020679:+33161 None:intergenic
TCGCTGAAACCTTATTCGAT+GGG 0.554144 tig0020679:+36084 None:intergenic
TTCTTAGCAACATCATAACT+AGG 0.560239 tig0020679:+35990 None:intergenic
GTTTGATATCTAGTTTGGGA+TGG 0.565984 tig0020679:-33638 Msa1384760:three_prime_UTR
GCGGCTTTCATTGTTAATGA+CGG 0.572630 tig0020679:+36498 None:intergenic
TTGAGTTCCTGCTGTTTGCG+CGG 0.574735 tig0020679:+33265 None:intergenic
ACAGAATAACTTCATGAGAA+GGG 0.575988 tig0020679:+33217 None:intergenic
TTTGGACGGAACATTTGTGT+GGG 0.592902 tig0020679:-36108 Msa1384760:CDS
GGGTTGGTGGTGAAAATGGG+AGG 0.595797 tig0020679:+36525 None:intergenic
ATCGCCGGCGTTGAAGGCGA+CGG 0.596432 tig0020679:+36428 None:intergenic
GTCGTATTTGCCCTTAACAG+CGG 0.599655 tig0020679:+36479 None:intergenic
TAGCGTCTGTTATCGATGCT+CGG 0.602349 tig0020679:+36397 None:intergenic
GGTCGAGGAATTCGAAACAA+AGG 0.607936 tig0020679:-35875 Msa1384760:CDS
TAGTCGATACTGAAGGAACC+GGG 0.608857 tig0020679:+36324 None:intergenic
TTAACCTACATACACAGCAA+AGG 0.618005 tig0020679:-36146 Msa1384760:CDS
GCCGGCGTTGAAGGCGACGG+AGG 0.618807 tig0020679:+36431 None:intergenic
AAATGGCAAAATATGCCTCA+TGG 0.624715 tig0020679:+33603 None:intergenic
GTAAGAAGTTAGGGTTTGAG+TGG 0.628064 tig0020679:-35896 Msa1384760:CDS
TCCTCATAAGAAAAGAGGAT+AGG 0.639269 tig0020679:-33367 Msa1384760:three_prime_UTR
TGATGTTGCTAAGAATGCGT+GGG 0.641670 tig0020679:-35982 Msa1384760:CDS
TCATTGTTAATGACGGAGCG+GGG 0.645523 tig0020679:+36505 None:intergenic
AAACCCTAACTTCTTACTCG+TGG 0.657874 tig0020679:+35902 None:intergenic
ATGATGTTGCTAAGAATGCG+TGG 0.662892 tig0020679:-35983 Msa1384760:CDS
GACGTTGTAGTCGATACTGA+AGG 0.665017 tig0020679:+36317 None:intergenic
CTACATTTCAAAATTCCATG+TGG 0.674623 tig0020679:+33671 None:intergenic
TTTCATTGTTAATGACGGAG+CGG 0.683309 tig0020679:+36503 None:intergenic
AACCTACATACACAGCAAAG+GGG 0.729150 tig0020679:-36144 Msa1384760:CDS
TCGAGGAATTCGAAACAAAG+GGG 0.745078 tig0020679:-35873 Msa1384760:CDS
CTTCCCCAACAGCAACACAG+AGG 0.753282 tig0020679:-33321 Msa1384760:three_prime_UTR
TCATGTAATGAACTACTCTG+TGG 0.767494 tig0020679:-33552 Msa1384760:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAATAAATTGAAC+CGG + tig0020679:33385-33404 None:intergenic 10.0%
!! AGAAAATTAACAAAATTATT+TGG - tig0020679:34104-34123 Msa1384760:intron 10.0%
!! TTAACAAAACTATTTAAAAA+AGG + tig0020679:35375-35394 None:intergenic 10.0%
!!! ATAATATTTTTCTTTTTTGA+AGG - tig0020679:35636-35655 Msa1384760:intron 10.0%
!!! ATTCTTTTTTTTTTTTTGAA+TGG - tig0020679:36218-36237 Msa1384760:intron 10.0%
!!! TTTTTTTTGTTTACCTTTTT+GGG + tig0020679:33338-33357 None:intergenic 15.0%
!!! TTTTTTTTTGTTTACCTTTT+TGG + tig0020679:33339-33358 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTGTGTG+TGG - tig0020679:33821-33840 Msa1384760:intron 15.0%
!!! AAAATCTTAGGTTTTATTTA+CGG + tig0020679:34002-34021 None:intergenic 15.0%
!! GAGTTATATATAAAAATCTT+AGG + tig0020679:34014-34033 None:intergenic 15.0%
!!! TTGATTTTAGTATATGTTAT+TGG - tig0020679:34040-34059 Msa1384760:intron 15.0%
!! ATATTATACCAATGTATTAT+TGG + tig0020679:34292-34311 None:intergenic 15.0%
!!! AGTTTTTATTCCTAAATAAA+TGG - tig0020679:34794-34813 Msa1384760:intron 15.0%
!!! GATTCATGTTTTTTTTTTTT+GGG + tig0020679:35332-35351 None:intergenic 15.0%
!!! TGATTCATGTTTTTTTTTTT+TGG + tig0020679:35333-35352 None:intergenic 15.0%
!!! ATGAATTTTAGAATAGTTTT+TGG - tig0020679:35516-35535 Msa1384760:intron 15.0%
!!! TGAATTTTAGAATAGTTTTT+GGG - tig0020679:35517-35536 Msa1384760:intron 15.0%
!!! TTTTTTTGTTTACCTTTTTG+GGG + tig0020679:33337-33356 None:intergenic 20.0%
!! TTTGATTATGGATTTGAATT+AGG - tig0020679:33846-33865 Msa1384760:intron 20.0%
!! TTGATTATGGATTTGAATTA+GGG - tig0020679:33847-33866 Msa1384760:intron 20.0%
!! TACTTAGTAAGAAACTAATT+TGG + tig0020679:34387-34406 None:intergenic 20.0%
!! ATTATAGTAATATGCTTAGA+AGG - tig0020679:34410-34429 Msa1384760:intron 20.0%
!!! AAAATGTACTGACATTATTT+TGG + tig0020679:34659-34678 None:intergenic 20.0%
!!! AGTACATTTTATGAAGAAAT+TGG - tig0020679:34669-34688 Msa1384760:intron 20.0%
!!! ATTTTATGAAGAAATTGGTT+GGG - tig0020679:34674-34693 Msa1384760:intron 20.0%
!! CAAATTCTATCCATTTATTT+AGG + tig0020679:34807-34826 None:intergenic 20.0%
!!! TAAATGGATAGAATTTGTTT+AGG - tig0020679:34810-34829 Msa1384760:intron 20.0%
!!! AAATGGATAGAATTTGTTTA+GGG - tig0020679:34811-34830 Msa1384760:intron 20.0%
!!! TTTGAAATGTAGTTTTCATA+AGG - tig0020679:35969-35988 Msa1384760:CDS 20.0%
!! TCAAGTATACAATAATGAAA+TGG + tig0020679:36046-36065 None:intergenic 20.0%
!! ATCAAATGAAAAGTTTAAAG+TGG + tig0020679:36113-36132 None:intergenic 20.0%
!! ATTTATAGGCAATCAATTAT+AGG + tig0020679:36174-36193 None:intergenic 20.0%
!! ATAATTGATTGCCTATAAAT+TGG - tig0020679:36174-36193 Msa1384760:CDS 20.0%
!! TAATTGATTGCCTATAAATT+GGG - tig0020679:36175-36194 Msa1384760:CDS 20.0%
! ATAAATAAATTGAACCGGTT+TGG + tig0020679:33380-33399 None:intergenic 25.0%
!!! TTTTTGTTTAATTTTGGTGC+AGG - tig0020679:33407-33426 Msa1384760:three_prime_UTR 25.0%
! TTCAATTTATGAACACAGTT+AGG - tig0020679:33439-33458 Msa1384760:three_prime_UTR 25.0%
! TCAATTTATGAACACAGTTA+GGG - tig0020679:33440-33459 Msa1384760:three_prime_UTR 25.0%
! TGTATGTAGGTTAAATTCAA+CGG + tig0020679:33477-33496 None:intergenic 25.0%
! GCATTTAACCAATAATACAT+TGG - tig0020679:34281-34300 Msa1384760:intron 25.0%
!!! AGATGTGTGAGTTTTTTAAA+GGG - tig0020679:34459-34478 Msa1384760:intron 25.0%
! TTCATTGTGATTCTTCATAT+TGG - tig0020679:34495-34514 Msa1384760:intron 25.0%
! CTTCTTAAATTCTTATTGTG+TGG + tig0020679:34532-34551 None:intergenic 25.0%
! CACACAATAAGAATTTAAGA+AGG - tig0020679:34530-34549 Msa1384760:intron 25.0%
! TTCATATGTATGTATGAAGA+CGG + tig0020679:34579-34598 None:intergenic 25.0%
!! CATTTTATGAAGAAATTGGT+TGG - tig0020679:34673-34692 Msa1384760:intron 25.0%
!! ATTTGTTTAGGGTTGAATAT+AGG - tig0020679:34822-34841 Msa1384760:intron 25.0%
! AAAGATAAAACCCAACTTAA+TGG + tig0020679:35260-35279 None:intergenic 25.0%
! ATAAGGTTTGATATCTAGTT+TGG - tig0020679:35986-36005 Msa1384760:CDS 25.0%
!! TAAGGTTTGATATCTAGTTT+GGG - tig0020679:35987-36006 Msa1384760:CDS 25.0%
! ATTATAGGATTCTGCAATAT+GGG + tig0020679:36159-36178 None:intergenic 25.0%
! AATTATAGGATTCTGCAATA+TGG + tig0020679:36160-36179 None:intergenic 25.0%
!!! AAGTAGCTGCTTTTATTTTT+TGG + tig0020679:36390-36409 None:intergenic 25.0%
!! TTTATGTGTTTTCGTTGAAT+CGG - tig0020679:36534-36553 Msa1384760:five_prime_UTR 25.0%
! ATCCATTTTCTAAACCAAAC+CGG - tig0020679:33363-33382 Msa1384760:three_prime_UTR 30.0%
!! TAATTTTGGTGCAGGATTTT+AGG - tig0020679:33415-33434 Msa1384760:three_prime_UTR 30.0%
! GATAACAGAACAATTTTGGA+CGG - tig0020679:33507-33526 Msa1384760:three_prime_UTR 30.0%
AATAAGGTTTCAGCGAATTA+TGG - tig0020679:33552-33571 Msa1384760:three_prime_UTR 30.0%
AAGTATAGCTATGTCCATAA+GGG - tig0020679:33600-33619 Msa1384760:three_prime_UTR 30.0%
TTCTTAGCAACATCATAACT+AGG + tig0020679:33642-33661 None:intergenic 30.0%
!!! TTGTGTGTGGATTTTGATTA+TGG - tig0020679:33834-33853 Msa1384760:intron 30.0%
TTGCAATTGTGGTTGAAATA+TGG - tig0020679:33933-33952 Msa1384760:intron 30.0%
!!! GTGATTACAATTCTGGTTTT+TGG - tig0020679:34081-34100 Msa1384760:intron 30.0%
GAACATTGGATTGATATCTA+TGG - tig0020679:34171-34190 Msa1384760:intron 30.0%
! AAACTAATTTGGAGTAATCC+CGG + tig0020679:34376-34395 None:intergenic 30.0%
TAATATGCTTAGAAGGTTGA+TGG - tig0020679:34417-34436 Msa1384760:intron 30.0%
!!! GAGATGTGTGAGTTTTTTAA+AGG - tig0020679:34458-34477 Msa1384760:intron 30.0%
! AGGACGTTTTACTTTATCTT+TGG - tig0020679:34617-34636 Msa1384760:intron 30.0%
! ACGTTTTACTTTATCTTTGG+AGG - tig0020679:34620-34639 Msa1384760:intron 30.0%
! CGTTTTACTTTATCTTTGGA+GGG - tig0020679:34621-34640 Msa1384760:intron 30.0%
!! GTTTTACTTTATCTTTGGAG+GGG - tig0020679:34622-34641 Msa1384760:intron 30.0%
GAATCAATTTCCAACTTCTT+TGG - tig0020679:34737-34756 Msa1384760:intron 30.0%
TGAAATAGCCAATACTTTAG+TGG - tig0020679:34883-34902 Msa1384760:intron 30.0%
! TGCTTGAAATCGATTTCAAA+TGG - tig0020679:35108-35127 Msa1384760:intron 30.0%
! TTTCAAATGGTAATGAAGTG+TGG - tig0020679:35121-35140 Msa1384760:intron 30.0%
GTTGAAGAAGATAGATGATT+GGG - tig0020679:35211-35230 Msa1384760:intron 30.0%
! TAAAACCCAACTTAATGGAT+CGG + tig0020679:35255-35274 None:intergenic 30.0%
TTTGGGTCAAATTTCCTTTA+CGG + tig0020679:35315-35334 None:intergenic 30.0%
CTACATTTCAAAATTCCATG+TGG + tig0020679:35961-35980 None:intergenic 30.0%
! GAATAAAGTGCCCAATTTAT+AGG + tig0020679:36188-36207 None:intergenic 30.0%
!!! GAGTTTTTTCTTTCTGTTAG+CGG + tig0020679:36336-36355 None:intergenic 30.0%
ACAGAATAACTTCATGAGAA+GGG + tig0020679:36415-36434 None:intergenic 30.0%
TACAGAATAACTTCATGAGA+AGG + tig0020679:36416-36435 None:intergenic 30.0%
!! TTGCAAGTATTGATTGACTT+GGG + tig0020679:36471-36490 None:intergenic 30.0%
! TTATGTGTTTTCGTTGAATC+GGG - tig0020679:36535-36554 Msa1384760:five_prime_UTR 30.0%
! TTTCATTGTTAATGACGGAG+CGG + tig0020679:33129-33148 None:intergenic 35.0%
! ACAGACGCTACTTTCATTAA+CGG - tig0020679:33245-33264 Msa1384760:three_prime_UTR 35.0%
!! AACCGGTTTGGTTTAGAAAA+TGG + tig0020679:33368-33387 None:intergenic 35.0%
TTAACCTACATACACAGCAA+AGG - tig0020679:33483-33502 Msa1384760:three_prime_UTR 35.0%
TAACCTACATACACAGCAAA+GGG - tig0020679:33484-33503 Msa1384760:three_prime_UTR 35.0%
! AGGGGATAACAGAACAATTT+TGG - tig0020679:33503-33522 Msa1384760:three_prime_UTR 35.0%
! CGAATTATGGTGTTGAATCT+GGG - tig0020679:33565-33584 Msa1384760:three_prime_UTR 35.0%
GAAGTATAGCTATGTCCATA+AGG - tig0020679:33599-33618 Msa1384760:three_prime_UTR 35.0%
CAATTGTAGTCAAACCCTTA+TGG + tig0020679:33617-33636 None:intergenic 35.0%
! TTATGGTGATGACTCATTGA+AGG - tig0020679:33692-33711 Msa1384760:CDS 35.0%
!! GAAACAAAGGGGTTGTTTTA+AGG - tig0020679:33767-33786 Msa1384760:intron 35.0%
ATGGATTTGAATTAGGGTTG+AGG - tig0020679:33853-33872 Msa1384760:intron 35.0%
TGAATTAGGGTTGAGGAATT+TGG - tig0020679:33860-33879 Msa1384760:intron 35.0%
! GTAGAAAATTGTTGTTGACG+TGG - tig0020679:33910-33929 Msa1384760:intron 35.0%
TTGTGGTTGAAATATGGTTG+TGG - tig0020679:33939-33958 Msa1384760:intron 35.0%
!! AGCTGCTTAGAATTGATTTG+TGG - tig0020679:34204-34223 Msa1384760:intron 35.0%
CAGAAGAAAGACAAAAGACA+TGG + tig0020679:34233-34252 None:intergenic 35.0%
! AACTAATTTGGAGTAATCCC+GGG + tig0020679:34375-34394 None:intergenic 35.0%
! ATGGCTATGTTTGAGATTGA+GGG - tig0020679:34436-34455 Msa1384760:intron 35.0%
! TTTGAGGAGGTACAAAACAA+CGG - tig0020679:34697-34716 Msa1384760:intron 35.0%
TCAATTTCCAACTTCTTTGG+TGG - tig0020679:34740-34759 Msa1384760:intron 35.0%
ATTCATACCACCAAAGAAGT+TGG + tig0020679:34750-34769 None:intergenic 35.0%
CAAGACTTCCACTAAAGTAT+TGG + tig0020679:34894-34913 None:intergenic 35.0%
AACATAATTCGCTGGGTTTA+TGG - tig0020679:34922-34941 Msa1384760:intron 35.0%
ACATAATTCGCTGGGTTTAT+GGG - tig0020679:34923-34942 Msa1384760:intron 35.0%
AAAGCTTACGTAAACGTTCA+TGG + tig0020679:35076-35095 None:intergenic 35.0%
GGTTGAAGAAGATAGATGAT+TGG - tig0020679:35210-35229 Msa1384760:intron 35.0%
! AAAACCCAACTTAATGGATC+GGG + tig0020679:35254-35273 None:intergenic 35.0%
TTTGTTCGAAAGACTCGTAT+AGG - tig0020679:35553-35572 Msa1384760:intron 35.0%
TTGTTCGAAAGACTCGTATA+GGG - tig0020679:35554-35573 Msa1384760:intron 35.0%
TGAGAAGAATTCGGTACATA+CGG + tig0020679:35757-35776 None:intergenic 35.0%
ACACAATTCGCAATTCGTTA+TGG + tig0020679:35809-35828 None:intergenic 35.0%
AATTGCGAATTGTGTGACTA+TGG - tig0020679:35814-35833 Msa1384760:intron 35.0%
TTCAAAATTCCATGTGGTCT+CGG + tig0020679:35955-35974 None:intergenic 35.0%
! GTTTGATATCTAGTTTGGGA+TGG - tig0020679:35991-36010 Msa1384760:CDS 35.0%
AAATGGCAAAATATGCCTCA+TGG + tig0020679:36029-36048 None:intergenic 35.0%
TCATGTAATGAACTACTCTG+TGG - tig0020679:36077-36096 Msa1384760:CDS 35.0%
GAGGGATGCATGAATAATAT+TGG + tig0020679:36244-36263 None:intergenic 35.0%
! ATCCTCTTTTCTTATGAGGA+GGG + tig0020679:36262-36281 None:intergenic 35.0%
! TATCCTCTTTTCTTATGAGG+AGG + tig0020679:36263-36282 None:intergenic 35.0%
TCCTCATAAGAAAAGAGGAT+AGG - tig0020679:36262-36281 Msa1384760:intron 35.0%
! TCCTATCCTCTTTTCTTATG+AGG + tig0020679:36266-36285 None:intergenic 35.0%
CAGAAAGAAAAAACTCCTAC+AGG - tig0020679:36340-36359 Msa1384760:CDS 35.0%
!!! CCTAGCATGTTTTAGAGAAA+AGG + tig0020679:36442-36461 None:intergenic 35.0%
!!! CCTTTTCTCTAAAACATGCT+AGG - tig0020679:36439-36458 Msa1384760:CDS 35.0%
!! CTTGCAAGTATTGATTGACT+TGG + tig0020679:36472-36491 None:intergenic 35.0%
! TTCATTGTTAATGACGGAGC+GGG + tig0020679:33128-33147 None:intergenic 40.0%
GCGGCTTTCATTGTTAATGA+CGG + tig0020679:33134-33153 None:intergenic 40.0%
AACAATGAAAGCCGCTGTTA+AGG - tig0020679:33139-33158 Msa1384760:three_prime_UTR 40.0%
ACAATGAAAGCCGCTGTTAA+GGG - tig0020679:33140-33159 Msa1384760:three_prime_UTR 40.0%
AACCTACATACACAGCAAAG+GGG - tig0020679:33485-33504 Msa1384760:three_prime_UTR 40.0%
! TTTTGGACGGAACATTTGTG+TGG - tig0020679:33520-33539 Msa1384760:three_prime_UTR 40.0%
TTTGGACGGAACATTTGTGT+GGG - tig0020679:33521-33540 Msa1384760:three_prime_UTR 40.0%
TCGCTGAAACCTTATTCGAT+GGG + tig0020679:33548-33567 None:intergenic 40.0%
TTCGCTGAAACCTTATTCGA+TGG + tig0020679:33549-33568 None:intergenic 40.0%
! GCGAATTATGGTGTTGAATC+TGG - tig0020679:33564-33583 Msa1384760:three_prime_UTR 40.0%
ATGATGTTGCTAAGAATGCG+TGG - tig0020679:33646-33665 Msa1384760:three_prime_UTR 40.0%
TGATGTTGCTAAGAATGCGT+GGG - tig0020679:33647-33666 Msa1384760:three_prime_UTR 40.0%
! GCTGTTTCGAGGAAAGTTTA+TGG - tig0020679:33675-33694 Msa1384760:CDS 40.0%
AGACCACGAGTAAGAAGTTA+GGG - tig0020679:33724-33743 Msa1384760:CDS 40.0%
AAACCCTAACTTCTTACTCG+TGG + tig0020679:33730-33749 None:intergenic 40.0%
GTAAGAAGTTAGGGTTTGAG+TGG - tig0020679:33733-33752 Msa1384760:intron 40.0%
GTCGAGGAATTCGAAACAAA+GGG - tig0020679:33755-33774 Msa1384760:intron 40.0%
TCGAGGAATTCGAAACAAAG+GGG - tig0020679:33756-33775 Msa1384760:intron 40.0%
TGTGGTACAGCTTGATGTTT+CGG - tig0020679:33957-33976 Msa1384760:intron 40.0%
TGATGTTTCGGCGACTATAA+TGG - tig0020679:33969-33988 Msa1384760:intron 40.0%
GCTGCATGTGATTACAATTC+TGG - tig0020679:34074-34093 Msa1384760:intron 40.0%
GCATCAGTGACTTAGAACAT+TGG - tig0020679:34157-34176 Msa1384760:intron 40.0%
! GATGGCTATGTTTGAGATTG+AGG - tig0020679:34435-34454 Msa1384760:intron 40.0%
TAAAGTAAAACGTCCTCCCT+CGG + tig0020679:34613-34632 None:intergenic 40.0%
GAAGAAATTGGTTGGGTTTG+AGG - tig0020679:34681-34700 Msa1384760:intron 40.0%
! TTGAGGAGGTACAAAACAAC+GGG - tig0020679:34698-34717 Msa1384760:intron 40.0%
AGGTACAAAACAACGGGATT+TGG - tig0020679:34704-34723 Msa1384760:intron 40.0%
GGTACAAAACAACGGGATTT+GGG - tig0020679:34705-34724 Msa1384760:intron 40.0%
GTACAAAACAACGGGATTTG+GGG - tig0020679:34706-34725 Msa1384760:intron 40.0%
AGCAGAGTAACATAATTCGC+TGG - tig0020679:34914-34933 Msa1384760:intron 40.0%
GCAGAGTAACATAATTCGCT+GGG - tig0020679:34915-34934 Msa1384760:intron 40.0%
!!! TCTTTTGCGTATTGATTCGC+TGG - tig0020679:34983-35002 Msa1384760:intron 40.0%
TTCATGGAATCGTCGTACTT+CGG + tig0020679:35041-35060 None:intergenic 40.0%
TTGAGACTGTTCAACCGTAA+AGG - tig0020679:35298-35317 Msa1384760:intron 40.0%
!! AGTTTTTGGGAAGTGAACAC+TGG - tig0020679:35530-35549 Msa1384760:intron 40.0%
AGAAGAATTCGGTACATACG+GGG + tig0020679:35755-35774 None:intergenic 40.0%
GAGAAGAATTCGGTACATAC+GGG + tig0020679:35756-35775 None:intergenic 40.0%
TACGCGACATGAGAAGAATT+CGG + tig0020679:35766-35785 None:intergenic 40.0%
! CTTGGGAATTTTCTTCTCCT+CGG + tig0020679:35925-35944 None:intergenic 40.0%
ATCCCTCCTCATAAGAAAAG+AGG - tig0020679:36257-36276 Msa1384760:intron 40.0%
! TCATTGTTAATGACGGAGCG+GGG + tig0020679:33127-33146 None:intergenic 45.0%
GTCGTATTTGCCCTTAACAG+CGG + tig0020679:33153-33172 None:intergenic 45.0%
TAGCGTCTGTTATCGATGCT+CGG + tig0020679:33235-33254 None:intergenic 45.0%
ATTAACGGCCCAAGCTTAAC+CGG - tig0020679:33260-33279 Msa1384760:three_prime_UTR 45.0%
! GAAGGAACCGGGTTTTTCTA+CGG + tig0020679:33297-33316 None:intergenic 45.0%
TAGTCGATACTGAAGGAACC+GGG + tig0020679:33308-33327 None:intergenic 45.0%
GTAGTCGATACTGAAGGAAC+CGG + tig0020679:33309-33328 None:intergenic 45.0%
GACGTTGTAGTCGATACTGA+AGG + tig0020679:33315-33334 None:intergenic 45.0%
! ATCCCCTTTGCTGTGTATGT+AGG + tig0020679:33490-33509 None:intergenic 45.0%
CAGACCACGAGTAAGAAGTT+AGG - tig0020679:33723-33742 Msa1384760:CDS 45.0%
GGTCGAGGAATTCGAAACAA+AGG - tig0020679:33754-33773 Msa1384760:intron 45.0%
TGTTGACGTGGTTGCAATTG+TGG - tig0020679:33922-33941 Msa1384760:intron 45.0%
CCGGGAGAAAAACAAGTACA+CGG + tig0020679:34357-34376 None:intergenic 45.0%
!! CCGTGTACTTGTTTTTCTCC+CGG - tig0020679:34354-34373 Msa1384760:intron 45.0%
!! CGTGTACTTGTTTTTCTCCC+GGG - tig0020679:34355-34374 Msa1384760:intron 45.0%
TATGAACAGAGAATCCTCCG+AGG - tig0020679:34593-34612 Msa1384760:intron 45.0%
ATGAACAGAGAATCCTCCGA+GGG - tig0020679:34594-34613 Msa1384760:intron 45.0%
GAAATTGGTTGGGTTTGAGG+AGG - tig0020679:34684-34703 Msa1384760:intron 45.0%
TTTATGGGAGAACCGCGAAT+TGG - tig0020679:34938-34957 Msa1384760:intron 45.0%
TACGCAAAAGAACCGCGAAT+CGG + tig0020679:34974-34993 None:intergenic 45.0%
CGATTCCATGAACGTGTTTG+AGG - tig0020679:35049-35068 Msa1384760:intron 45.0%
ATGGTCCTCAAACACGTTCA+TGG + tig0020679:35057-35076 None:intergenic 45.0%
TGGATCGGGTGCAAACATAT+GGG + tig0020679:35240-35259 None:intergenic 45.0%
ATGGATCGGGTGCAAACATA+TGG + tig0020679:35241-35260 None:intergenic 45.0%
TTGCACCCGATCCATTAAGT+TGG - tig0020679:35246-35265 Msa1384760:intron 45.0%
TGCACCCGATCCATTAAGTT+GGG - tig0020679:35247-35266 Msa1384760:intron 45.0%
GAAGAATTCGGTACATACGG+GGG + tig0020679:35754-35773 None:intergenic 45.0%
ATTCGTTATGGTACGCGGTA+CGG + tig0020679:35797-35816 None:intergenic 45.0%
TCGCAATTCGTTATGGTACG+CGG + tig0020679:35802-35821 None:intergenic 45.0%
CTATCAGCTATGTCTCTTGC+AGG - tig0020679:35878-35897 Msa1384760:CDS 45.0%
!! CTAGTTTGGGATGGCTTTTG+TGG - tig0020679:36000-36019 Msa1384760:CDS 45.0%
AGCGCAACAGCAAAAAAAGC+AGG - tig0020679:36285-36304 Msa1384760:intron 45.0%
!!! TATTTATTTTTGTTTAATTT+TGG - tig0020679:33401-33420 Msa1384760:three_prime_UTR 5.0%
!!! TCATTTATTTTTATTTTTAT+TGG + tig0020679:35500-35519 None:intergenic 5.0%
GGACTAAGCCGGTTAAGCTT+GGG + tig0020679:33271-33290 None:intergenic 50.0%
AGGACTAAGCCGGTTAAGCT+TGG + tig0020679:33272-33291 None:intergenic 50.0%
CGACTACAACGTCCCCAAAA+AGG - tig0020679:33322-33341 Msa1384760:three_prime_UTR 50.0%
TGTGTGGGACCCATCGAATA+AGG - tig0020679:33536-33555 Msa1384760:three_prime_UTR 50.0%
!!! CGTGGGATTTTGCTGTTTCG+AGG - tig0020679:33664-33683 Msa1384760:exon 50.0%
AGTTAGGGTTTGAGTGGTCG+AGG - tig0020679:33739-33758 Msa1384760:intron 50.0%
TGATTCGCTGGAATTCGTGC+CGG - tig0020679:34995-35014 Msa1384760:intron 50.0%
! CCATGTGCTGTGTTTTCCTG+CGG - tig0020679:35168-35187 Msa1384760:intron 50.0%
! GGCTGCACTTCTGTTTTGCT+AGG - tig0020679:35189-35208 Msa1384760:intron 50.0%
ATCGGGTGCAAACATATGGG+TGG + tig0020679:35237-35256 None:intergenic 50.0%
ATTCGGTACATACGGGGGTA+TGG + tig0020679:35749-35768 None:intergenic 50.0%
TTCGTTATGGTACGCGGTAC+GGG + tig0020679:35796-35815 None:intergenic 50.0%
GCTATGTCTCTTGCAGGTTG+TGG - tig0020679:35884-35903 Msa1384760:CDS 50.0%
CTTCTCCTCGGCCAAGTTAA+CGG + tig0020679:35913-35932 None:intergenic 50.0%
! TGGCTTTTGTGGTCACCATG+AGG - tig0020679:36011-36030 Msa1384760:CDS 50.0%
! TTGAGTTCCTGCTGTTTGCG+CGG + tig0020679:36367-36386 None:intergenic 50.0%
!! GGGTTGGTGGTGAAAATGGG+AGG + tig0020679:33107-33126 None:intergenic 55.0%
!! GCGGGGTTGGTGGTGAAAAT+GGG + tig0020679:33110-33129 None:intergenic 55.0%
!! AGCGGGGTTGGTGGTGAAAA+TGG + tig0020679:33111-33130 None:intergenic 55.0%
! TGTTAATGACGGAGCGGGGT+TGG + tig0020679:33123-33142 None:intergenic 55.0%
! TTTGACATCGCCGGCGTTGA+AGG + tig0020679:33210-33229 None:intergenic 55.0%
TGCTCGGAGTTTGACATCGC+CGG + tig0020679:33219-33238 None:intergenic 55.0%
TTCTACGGCGAGGACTAAGC+CGG + tig0020679:33282-33301 None:intergenic 55.0%
GTCCTCGCCGTAGAAAAACC+CGG - tig0020679:33287-33306 Msa1384760:three_prime_UTR 55.0%
! AACCGGGTTTTTCTACGGCG+AGG + tig0020679:33292-33311 None:intergenic 55.0%
AACAGAGAATCCTCCGAGGG+AGG - tig0020679:34597-34616 Msa1384760:intron 55.0%
AGTAAAACGTCCTCCCTCGG+AGG + tig0020679:34610-34629 None:intergenic 55.0%
CCGCAGGAAAACACAGCACA+TGG + tig0020679:35171-35190 None:intergenic 55.0%
! CAAAACAGAAGTGCAGCCGC+AGG + tig0020679:35187-35206 None:intergenic 55.0%
! CATACGGGGGTATGGAGCTT+CGG + tig0020679:35741-35760 None:intergenic 55.0%
GGTTGTGGCGTCCGTTAACT+TGG - tig0020679:35899-35918 Msa1384760:CDS 55.0%
GTGGTCTCGGCACTAAGCTT+GGG + tig0020679:35942-35961 None:intergenic 55.0%
TGTGGTCTCGGCACTAAGCT+TGG + tig0020679:35943-35962 None:intergenic 55.0%
! AGCTTAGTGCCGAGACCACA+TGG - tig0020679:35943-35962 Msa1384760:CDS 55.0%
CTTCCCCAACAGCAACACAG+AGG - tig0020679:36308-36327 Msa1384760:CDS 55.0%
! GCTCCTCTGTGTTGCTGTTG+GGG + tig0020679:36314-36333 None:intergenic 55.0%
! GGCTCCTCTGTGTTGCTGTT+GGG + tig0020679:36315-36334 None:intergenic 55.0%
! TGAGTTCCTGCTGTTTGCGC+GGG + tig0020679:36366-36385 None:intergenic 55.0%
! TAATGACGGAGCGGGGTTGG+TGG + tig0020679:33120-33139 None:intergenic 60.0%
GGTACGCGCGAACCAATTCG+CGG + tig0020679:34953-34972 None:intergenic 60.0%
TCGCTGAGTTGCGCGGATTC+CGG + tig0020679:35017-35036 None:intergenic 60.0%
! CTTCGGTTCGCTGAGTTGCG+CGG + tig0020679:35024-35043 None:intergenic 60.0%
! CGGCTCCTCTGTGTTGCTGT+TGG + tig0020679:36316-36335 None:intergenic 60.0%
GTTGAAGGCGACGGAGGCTG+CGG + tig0020679:33195-33214 None:intergenic 65.0%
! ATCGCCGGCGTTGAAGGCGA+CGG + tig0020679:33204-33223 None:intergenic 65.0%
!! GGTTCGCGCGTACCGATTCG+CGG - tig0020679:34959-34978 Msa1384760:intron 65.0%
TGGTACGCGGTACGGGTACG+CGG + tig0020679:35789-35808 None:intergenic 65.0%
! GGCGTCCGTTAACTTGGCCG+AGG - tig0020679:35905-35924 Msa1384760:CDS 65.0%
! TGCTGTTTGCGCGGGCCTGT+AGG + tig0020679:36358-36377 None:intergenic 65.0%
TACAGGCCCGCGCAAACAGC+AGG - tig0020679:36357-36376 Msa1384760:CDS 65.0%
GCCTCCGTCGCCTTCAACGC+CGG - tig0020679:33197-33216 Msa1384760:three_prime_UTR 70.0%
GAAGGCGACGGAGGCTGCGG+CGG + tig0020679:33192-33211 None:intergenic 75.0%
! GCCGGCGTTGAAGGCGACGG+AGG + tig0020679:33201-33220 None:intergenic 75.0%
! GGCGACGGAGGCTGCGGCGG+CGG + tig0020679:33189-33208 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
tig0020679 gene 33085 36566 33085 ID=Msa1384760;Name=Msa1384760
tig0020679 mRNA 33085 36566 33085 ID=Msa1384760-mRNA-1;Parent=Msa1384760;Name=Msa1384760-mRNA-1;_AED=0.00;_eAED=0.00;_QI=58|1|1|1|1|1|3|586|213
tig0020679 exon 33085 33751 33085 ID=Msa1384760-mRNA-1:exon:9245;Parent=Msa1384760-mRNA-1
tig0020679 exon 35863 36222 35863 ID=Msa1384760-mRNA-1:exon:9244;Parent=Msa1384760-mRNA-1
tig0020679 exon 36308 36566 36308 ID=Msa1384760-mRNA-1:exon:9243;Parent=Msa1384760-mRNA-1
tig0020679 five_prime_UTR 36509 36566 36509 ID=Msa1384760-mRNA-1:five_prime_utr;Parent=Msa1384760-mRNA-1
tig0020679 CDS 36308 36508 36308 ID=Msa1384760-mRNA-1:cds;Parent=Msa1384760-mRNA-1
tig0020679 CDS 35863 36222 35863 ID=Msa1384760-mRNA-1:cds;Parent=Msa1384760-mRNA-1
tig0020679 CDS 33671 33751 33671 ID=Msa1384760-mRNA-1:cds;Parent=Msa1384760-mRNA-1
tig0020679 three_prime_UTR 33085 33670 33085 ID=Msa1384760-mRNA-1:three_prime_utr;Parent=Msa1384760-mRNA-1
Gene Sequence

>Msa1384760

ATGAAAGCCGCTGTTAAGGGCAAATACGACAATGACAAAAACGCCGCCGCCGCAGCCTCCGTCGCCTTCAACGCCGGCGATGTCAAACTCCGAGCATCGATAACAGACGCTACTTTCATTAACGGCCCAAGCTTAACCGGCTTAGTCCTCGCCGTAGAAAAACCCGGTTCCTTCAGTATCGACTACAACGTCCCCAAAAAGGATTTTAGGTTTCAATTTATGAACACAGTTAGGGTTGCTGATAAACCGTTGAATTTAACCTACATACACAGCAAAGGGGATAACAGAACAATTTTGGACGGAACATTTGTGTGGGACCCATCGAATAAGGTTTCAGCGAATTATGGTGTTGAATCTGGGAATTGTAGATTGAAGTATAGCTATGTCCATAAGGGTTTGACTACAATTGAACCTAGTTATGATGTTGCTAAGAATGCGTGGGATTTTGCTGTTTCGAGGAAAGTTTATGGTGATGACTCATTGAAGGCGACGTATCAGACCACGAGTAAGAAGTTAGGGTTTGAGTGGTCGAGGAATTCGAAACAAAGGGGTTGTTTTAAGGTTGTGGCGTCCGTTAACTTGGCCGAGGAGAAGAAAATTCCCAAGCTTAGTGCCGAGACCACATGGAATTTTGAAATGTAG
Protein sequence

>Msa1384760

MKAAVKGKYDNDKNAAAAASVAFNAGDVKLRASITDATFINGPSLTGLVLAVEKPGSFSIDYNVPKKDFRFQFMNTVRVADKPLNLTYIHSKGDNRTILDGTFVWDPSNKVSANYGVESGNCRLKYSYVHKGLTTIEPSYDVAKNAWDFAVSRKVYGDDSLKATYQTTSKKLGFEWSRNSKQRGCFKVVASVNLAEEKKIPKLSAETTWNFEM*