AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0101500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101500 MtrunA17_Chr1g0154751 100.000 213 0 0 1 213 1 213 1.87e-159 439
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101500 AT1G45170.1 69.484 213 64 1 1 213 2 213 8.28e-111 316
Msa0101500 AT5G42960.1 68.075 213 66 2 1 213 3 213 5.09e-109 311
Msa0101500 AT1G45170.2 55.399 213 48 2 1 213 2 167 1.92e-76 228

Find 94 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTGATTGCCTATAAAT+TGG 0.165071 1_3:+8148159 Msa0101500:three_prime_UTR
GCGAATTATGGTGTTGAATC+TGG 0.218241 1_3:+8145453 Msa0101500:CDS
AGGGGATAACAGAACAATTT+TGG 0.234866 1_3:+8145392 Msa0101500:CDS
AATTATAGGATTCTGCAATA+TGG 0.241988 1_3:-8148142 None:intergenic
ATAAGGTTTGATATCTAGTT+TGG 0.262212 1_3:+8147971 Msa0101500:three_prime_UTR
ATTTATAGGCAATCAATTAT+AGG 0.279411 1_3:-8148156 None:intergenic
GAATAAAGTGCCCAATTTAT+AGG 0.281680 1_3:-8148170 None:intergenic
AATAAGGTTTCAGCGAATTA+TGG 0.297969 1_3:+8145441 Msa0101500:CDS
GGCTCCTCTGTGTTGCTGTT+GGG 0.309390 1_3:-8148298 None:intergenic
CAATTGTAGTCAAACCCTTA+TGG 0.311402 1_3:-8145503 None:intergenic
TTCAATTTATGAACACAGTT+AGG 0.317886 1_3:+8145328 Msa0101500:CDS
GGCGACGGAGGCTGCGGCGG+CGG 0.338930 1_3:-8145071 None:intergenic
AACAATGAAAGCCGCTGTTA+AGG 0.348424 1_3:+8145024 Msa0101500:exon
GCTGTTTCGAGGAAAGTTTA+TGG 0.364076 1_3:+8145564 Msa0101500:CDS
CTTCTCCTCGGCCAAGTTAA+CGG 0.367147 1_3:-8147895 None:intergenic
TTCAAAATTCCATGTGGTCT+CGG 0.373502 1_3:-8147937 None:intergenic
ACAATGAAAGCCGCTGTTAA+GGG 0.378379 1_3:+8145025 Msa0101500:exon
TGTGTGGGACCCATCGAATA+AGG 0.383360 1_3:+8145425 Msa0101500:CDS
CGACTACAACGTCCCCAAAA+AGG 0.387317 1_3:+8145207 Msa0101500:CDS
GAGGGATGCATGAATAATAT+TGG 0.392922 1_3:-8148226 None:intergenic
CAGACCACGAGTAAGAAGTT+AGG 0.393154 1_3:+8145612 Msa0101500:CDS
ATTAACGGCCCAAGCTTAAC+CGG 0.393393 1_3:+8145145 Msa0101500:CDS
ACAGACGCTACTTTCATTAA+CGG 0.400228 1_3:+8145130 Msa0101500:CDS
GCCTCCGTCGCCTTCAACGC+CGG 0.402791 1_3:+8145082 Msa0101500:CDS
TAAGGTTTGATATCTAGTTT+GGG 0.410066 1_3:+8147972 Msa0101500:three_prime_UTR
GGTTGTGGCGTCCGTTAACT+TGG 0.413701 1_3:+8147884 Msa0101500:intron
TAATTGATTGCCTATAAATT+GGG 0.414424 1_3:+8148160 Msa0101500:three_prime_UTR
TTTGACATCGCCGGCGTTGA+AGG 0.416806 1_3:-8145092 None:intergenic
CGGCTCCTCTGTGTTGCTGT+TGG 0.426162 1_3:-8148299 None:intergenic
ATTATAGGATTCTGCAATAT+GGG 0.432310 1_3:-8148141 None:intergenic
TAACCTACATACACAGCAAA+GGG 0.443140 1_3:+8145373 Msa0101500:CDS
TGAGTTCCTGCTGTTTGCGC+GGG 0.443439 1_3:-8148349 None:intergenic
TGTGGTCTCGGCACTAAGCT+TGG 0.445547 1_3:-8147925 None:intergenic
GAAGTATAGCTATGTCCATA+AGG 0.446086 1_3:+8145488 Msa0101500:CDS
TCAATTTATGAACACAGTTA+GGG 0.463428 1_3:+8145329 Msa0101500:CDS
TCAAGTATACAATAATGAAA+TGG 0.464664 1_3:-8148028 None:intergenic
GTGGTCTCGGCACTAAGCTT+GGG 0.465342 1_3:-8147924 None:intergenic
AGGACTAAGCCGGTTAAGCT+TGG 0.467012 1_3:-8145154 None:intergenic
GCGGAGTTGGTGGTGAAAAT+CGG 0.467527 1_3:-8144992 None:intergenic
GTAGTCGATACTGAAGGAAC+CGG 0.468560 1_3:-8145191 None:intergenic
AAGTATAGCTATGTCCATAA+GGG 0.470434 1_3:+8145489 Msa0101500:CDS
GGACTAAGCCGGTTAAGCTT+GGG 0.473049 1_3:-8145153 None:intergenic
TGCTGTTTGCGCGGGCCTGT+AGG 0.474058 1_3:-8148341 None:intergenic
CGAATTATGGTGTTGAATCT+GGG 0.476174 1_3:+8145454 Msa0101500:CDS
TACAGGCCCGCGCAAACAGC+AGG 0.477584 1_3:+8148343 Msa0101500:three_prime_UTR
AGTTAGGGTTTGAGTGGTCG+AGG 0.482159 1_3:+8145628 Msa0101500:CDS
ATCAAATGAAAAGTTTAAAG+TGG 0.483426 1_3:-8148095 None:intergenic
CTTGCAAGTATTGATTGACT+TGG 0.493911 1_3:-8148455 None:intergenic
ATCCCCTTTGCTGTGTATGT+AGG 0.496851 1_3:-8145376 None:intergenic
GCTCCTCTGTGTTGCTGTTG+GGG 0.497041 1_3:-8148297 None:intergenic
AGACCACGAGTAAGAAGTTA+GGG 0.497058 1_3:+8145613 Msa0101500:CDS
TGTTAATGACGGAGCGGAGT+TGG 0.498274 1_3:-8145005 None:intergenic
TTCGCTGAAACCTTATTCGA+TGG 0.499631 1_3:-8145435 None:intergenic
GTTGAAGGCGACGGAGGCTG+CGG 0.500163 1_3:-8145077 None:intergenic
GTCGAGGAATTCGAAACAAA+GGG 0.505059 1_3:+8145644 Msa0101500:CDS
ATCCCTCCTCATAAGAAAAG+AGG 0.512029 1_3:+8148242 Msa0101500:three_prime_UTR
TTCTACGGCGAGGACTAAGC+CGG 0.514419 1_3:-8145164 None:intergenic
TTATGGTGATGACTCATTGA+AGG 0.523909 1_3:+8145581 Msa0101500:CDS
TACAGAATAACTTCATGAGA+AGG 0.528811 1_3:-8148399 None:intergenic
GAAGGCGACGGAGGCTGCGG+CGG 0.533980 1_3:-8145074 None:intergenic
TGCTCGGAGTTTGACATCGC+CGG 0.539580 1_3:-8145101 None:intergenic
GGCGTCCGTTAACTTGGCCG+AGG 0.541591 1_3:+8147890 Msa0101500:CDS
TGTATGTAGGTTAAATTCAA+CGG 0.544412 1_3:-8145363 None:intergenic
AGCTTAGTGCCGAGACCACA+TGG 0.549160 1_3:+8147928 Msa0101500:CDS
TTGCAAGTATTGATTGACTT+GGG 0.550118 1_3:-8148454 None:intergenic
TCGCTGAAACCTTATTCGAT+GGG 0.554144 1_3:-8145434 None:intergenic
TTCTTAGCAACATCATAACT+AGG 0.560239 1_3:-8145528 None:intergenic
GTTTGATATCTAGTTTGGGA+TGG 0.565984 1_3:+8147976 Msa0101500:three_prime_UTR
GCGGCTTTCATTGTTAATGA+CGG 0.572630 1_3:-8145016 None:intergenic
TTGAGTTCCTGCTGTTTGCG+CGG 0.574735 1_3:-8148350 None:intergenic
ACAGAATAACTTCATGAGAA+GGG 0.575988 1_3:-8148398 None:intergenic
TTTGGACGGAACATTTGTGT+GGG 0.592902 1_3:+8145410 Msa0101500:CDS
ATCGCCGGCGTTGAAGGCGA+CGG 0.596432 1_3:-8145086 None:intergenic
GTCGTATTTGCCCTTAACAG+CGG 0.599655 1_3:-8145035 None:intergenic
TAGCGTCTGTTATCGATGCT+CGG 0.602349 1_3:-8145117 None:intergenic
TAATGACGGAGCGGAGTTGG+TGG 0.605891 1_3:-8145002 None:intergenic
GGTCGAGGAATTCGAAACAA+AGG 0.607936 1_3:+8145643 Msa0101500:CDS
TAGTCGATACTGAAGGAACC+GGG 0.608857 1_3:-8145190 None:intergenic
TTAACCTACATACACAGCAA+AGG 0.618005 1_3:+8145372 Msa0101500:CDS
GCCGGCGTTGAAGGCGACGG+AGG 0.618807 1_3:-8145083 None:intergenic
AAATGGCAAAATATGCCTCA+TGG 0.624715 1_3:-8148011 None:intergenic
GTAAGAAGTTAGGGTTTGAG+TGG 0.628064 1_3:+8145622 Msa0101500:CDS
TCCTCATAAGAAAAGAGGAT+AGG 0.639269 1_3:+8148247 Msa0101500:three_prime_UTR
TGATGTTGCTAAGAATGCGT+GGG 0.641670 1_3:+8145536 Msa0101500:CDS
AAACCCTAACTTCTTACTCG+TGG 0.657874 1_3:-8145616 None:intergenic
ATGATGTTGCTAAGAATGCG+TGG 0.662892 1_3:+8145535 Msa0101500:CDS
GACGTTGTAGTCGATACTGA+AGG 0.665017 1_3:-8145197 None:intergenic
GAGTTGGTGGTGAAAATCGG+AGG 0.670099 1_3:-8144989 None:intergenic
CTACATTTCAAAATTCCATG+TGG 0.674623 1_3:-8147943 None:intergenic
TTTCATTGTTAATGACGGAG+CGG 0.708711 1_3:-8145011 None:intergenic
AACCTACATACACAGCAAAG+GGG 0.729150 1_3:+8145374 Msa0101500:CDS
TCGAGGAATTCGAAACAAAG+GGG 0.745078 1_3:+8145645 Msa0101500:CDS
CTTCCCCAACAGCAACACAG+AGG 0.753282 1_3:+8148294 Msa0101500:three_prime_UTR
TCATGTAATGAACTACTCTG+TGG 0.767494 1_3:+8148062 Msa0101500:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAATAAATTGAAC+CGG - chr1_3:8145270-8145289 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTTTTGTG+TGG + chr1_3:8145709-8145728 Msa0101500:intron 10.0%
!! AGAAAATTAACAAAATTATT+TGG + chr1_3:8145992-8146011 Msa0101500:intron 10.0%
!!! ATTCATGTTTTTTTTTTTTT+GGG - chr1_3:8147307-8147326 None:intergenic 10.0%
!! TTAACAAAACTATTTAAAAA+AGG - chr1_3:8147351-8147370 None:intergenic 10.0%
!!! ATAATATTTTTCTTTTTTGA+AGG + chr1_3:8147615-8147634 Msa0101500:intron 10.0%
!!! ATTCTTTTTTTTTTTTTGAA+TGG + chr1_3:8148203-8148222 Msa0101500:three_prime_UTR 10.0%
!!! TTTTTTTTGTTTACCTTTTT+GGG - chr1_3:8145223-8145242 None:intergenic 15.0%
!!! TTTTTTTTTGTTTACCTTTT+TGG - chr1_3:8145224-8145243 None:intergenic 15.0%
!!! AAAATCTTAGGTTTTATTTA+CGG - chr1_3:8145890-8145909 None:intergenic 15.0%
!! GAGTTATATATAAAAATCTT+AGG - chr1_3:8145902-8145921 None:intergenic 15.0%
!!! TTGATTTTAGTATATGTTAT+TGG + chr1_3:8145928-8145947 Msa0101500:intron 15.0%
!! ATATTATACCAATGTATTAT+TGG - chr1_3:8146167-8146186 None:intergenic 15.0%
!!! AGTTTTTATTCCTAAATAAA+TGG + chr1_3:8146669-8146688 Msa0101500:intron 15.0%
!!! GATTCATGTTTTTTTTTTTT+TGG - chr1_3:8147308-8147327 None:intergenic 15.0%
!!! ATGAATTTTAGAATAGTTTT+TGG + chr1_3:8147494-8147513 Msa0101500:intron 15.0%
!!! TGAATTTTAGAATAGTTTTT+GGG + chr1_3:8147495-8147514 Msa0101500:intron 15.0%
!!! TTTTTTTGTTTACCTTTTTG+GGG - chr1_3:8145222-8145241 None:intergenic 20.0%
!!! TTTGATTATGGTTTTGAATT+AGG + chr1_3:8145734-8145753 Msa0101500:intron 20.0%
!!! TTGATTATGGTTTTGAATTA+GGG + chr1_3:8145735-8145754 Msa0101500:intron 20.0%
!! TACTTAGTAAGAAACTAATT+TGG - chr1_3:8146262-8146281 None:intergenic 20.0%
!! ATTATAGTAATATGCTTAGA+AGG + chr1_3:8146285-8146304 Msa0101500:intron 20.0%
!!! AAAATGTACTGACATTATTT+TGG - chr1_3:8146534-8146553 None:intergenic 20.0%
!!! AGTACATTTTATGAAGAAAT+TGG + chr1_3:8146544-8146563 Msa0101500:intron 20.0%
!!! ATTTTATGAAGAAATTGGTT+GGG + chr1_3:8146549-8146568 Msa0101500:intron 20.0%
!! CAAATTCTATCCATTTATTT+AGG - chr1_3:8146682-8146701 None:intergenic 20.0%
!!! TAAATGGATAGAATTTGTTT+AGG + chr1_3:8146685-8146704 Msa0101500:intron 20.0%
!!! AAATGGATAGAATTTGTTTA+GGG + chr1_3:8146686-8146705 Msa0101500:intron 20.0%
!!! TTTGAAATGTAGTTTTCATA+AGG + chr1_3:8147954-8147973 Msa0101500:exon 20.0%
!! TCAAGTATACAATAATGAAA+TGG - chr1_3:8148031-8148050 None:intergenic 20.0%
!! ATCAAATGAAAAGTTTAAAG+TGG - chr1_3:8148098-8148117 None:intergenic 20.0%
!! ATTTATAGGCAATCAATTAT+AGG - chr1_3:8148159-8148178 None:intergenic 20.0%
!! ATAATTGATTGCCTATAAAT+TGG + chr1_3:8148159-8148178 Msa0101500:three_prime_UTR 20.0%
!! TAATTGATTGCCTATAAATT+GGG + chr1_3:8148160-8148179 Msa0101500:three_prime_UTR 20.0%
!!! TTTATGTGTTTTTGTTGAAT+CGG + chr1_3:8148520-8148539 Msa0101500:three_prime_UTR 20.0%
! ATAAATAAATTGAACCGGTT+TGG - chr1_3:8145265-8145284 None:intergenic 25.0%
!!! TTTTTGTTTAATTTTGGTGC+AGG + chr1_3:8145296-8145315 Msa0101500:intron 25.0%
! TTCAATTTATGAACACAGTT+AGG + chr1_3:8145328-8145347 Msa0101500:CDS 25.0%
! TCAATTTATGAACACAGTTA+GGG + chr1_3:8145329-8145348 Msa0101500:CDS 25.0%
! TGTATGTAGGTTAAATTCAA+CGG - chr1_3:8145366-8145385 None:intergenic 25.0%
!!! TTTTGTGTGGATTTTGATTA+TGG + chr1_3:8145722-8145741 Msa0101500:intron 25.0%
! GCATTTAACCAATAATACAT+TGG + chr1_3:8146156-8146175 Msa0101500:intron 25.0%
!!! AGATGTGTGAGTTTTTTAAA+GGG + chr1_3:8146334-8146353 Msa0101500:intron 25.0%
! TTCATTGTGATTCTTCATAT+TGG + chr1_3:8146370-8146389 Msa0101500:intron 25.0%
! CTTCTTAAATTCTTATTGTG+TGG - chr1_3:8146407-8146426 None:intergenic 25.0%
! CACACAATAAGAATTTAAGA+AGG + chr1_3:8146405-8146424 Msa0101500:intron 25.0%
! TTCATATGTATGTATGAAGA+CGG - chr1_3:8146454-8146473 None:intergenic 25.0%
!! CATTTTATGAAGAAATTGGT+TGG + chr1_3:8146548-8146567 Msa0101500:intron 25.0%
!! ATTTGTTTAGGGTTGAATAT+AGG + chr1_3:8146697-8146716 Msa0101500:intron 25.0%
! NNNNNTTACGTAAACGTTCA+TGG - chr1_3:8146951-8146970 None:intergenic 25.0%
! ATAAGGTTTGATATCTAGTT+TGG + chr1_3:8147971-8147990 Msa0101500:three_prime_UTR 25.0%
!! TAAGGTTTGATATCTAGTTT+GGG + chr1_3:8147972-8147991 Msa0101500:three_prime_UTR 25.0%
! ATTATAGGATTCTGCAATAT+GGG - chr1_3:8148144-8148163 None:intergenic 25.0%
! AATTATAGGATTCTGCAATA+TGG - chr1_3:8148145-8148164 None:intergenic 25.0%
!!! AAGTAGCTGCTTTTATTTTT+TGG - chr1_3:8148376-8148395 None:intergenic 25.0%
!!! TTATGTGTTTTTGTTGAATC+GGG + chr1_3:8148521-8148540 Msa0101500:three_prime_UTR 25.0%
! ATCCATTTTCTAAACCAAAC+CGG + chr1_3:8145248-8145267 Msa0101500:intron 30.0%
!! TAATTTTGGTGCAGGATTTT+AGG + chr1_3:8145304-8145323 Msa0101500:intron 30.0%
! GATAACAGAACAATTTTGGA+CGG + chr1_3:8145396-8145415 Msa0101500:CDS 30.0%
AATAAGGTTTCAGCGAATTA+TGG + chr1_3:8145441-8145460 Msa0101500:CDS 30.0%
AAGTATAGCTATGTCCATAA+GGG + chr1_3:8145489-8145508 Msa0101500:CDS 30.0%
TTCTTAGCAACATCATAACT+AGG - chr1_3:8145531-8145550 None:intergenic 30.0%
TTGCAATTGTGGTTGAAATA+TGG + chr1_3:8145821-8145840 Msa0101500:intron 30.0%
!!! GTGATTACAATTCTGGTTTT+TGG + chr1_3:8145969-8145988 Msa0101500:intron 30.0%
! AAACTAATTTGGAGTAATCC+CGG - chr1_3:8146251-8146270 None:intergenic 30.0%
TAATATGCTTAGAAGGTTGA+TGG + chr1_3:8146292-8146311 Msa0101500:intron 30.0%
!!! GAGATGTGTGAGTTTTTTAA+AGG + chr1_3:8146333-8146352 Msa0101500:intron 30.0%
! AGGACGTTTTACTTTATCTT+TGG + chr1_3:8146492-8146511 Msa0101500:intron 30.0%
! ACGTTTTACTTTATCTTTGG+AGG + chr1_3:8146495-8146514 Msa0101500:intron 30.0%
! CGTTTTACTTTATCTTTGGA+GGG + chr1_3:8146496-8146515 Msa0101500:intron 30.0%
!! GTTTTACTTTATCTTTGGAG+GGG + chr1_3:8146497-8146516 Msa0101500:intron 30.0%
GAATCAATTTCCAACTTCTT+TGG + chr1_3:8146612-8146631 Msa0101500:intron 30.0%
TGAAATAGCCAATACTTTAG+TGG + chr1_3:8146758-8146777 Msa0101500:intron 30.0%
! TGCTTGAAATCGATTTCAAA+TGG + chr1_3:8147083-8147102 Msa0101500:intron 30.0%
! TTTCAAATGGTAATGAAGTG+TGG + chr1_3:8147096-8147115 Msa0101500:intron 30.0%
GTTGAAGAAGATAGATGATT+GGG + chr1_3:8147186-8147205 Msa0101500:intron 30.0%
! TAAAACCCAACTTAATGGAT+CGG - chr1_3:8147230-8147249 None:intergenic 30.0%
CAAGATAAAACCCAACTTAA+TGG - chr1_3:8147235-8147254 None:intergenic 30.0%
!!! CATTAAGTTGGGTTTTATCT+TGG + chr1_3:8147233-8147252 Msa0101500:intron 30.0%
TTTGGGTCAAATTTCCTTTA+CGG - chr1_3:8147290-8147309 None:intergenic 30.0%
CTACATTTCAAAATTCCATG+TGG - chr1_3:8147946-8147965 None:intergenic 30.0%
! GAATAAAGTGCCCAATTTAT+AGG - chr1_3:8148173-8148192 None:intergenic 30.0%
!!! GAGTTTTTTCTTTCTGTTAG+CGG - chr1_3:8148322-8148341 None:intergenic 30.0%
ACAGAATAACTTCATGAGAA+GGG - chr1_3:8148401-8148420 None:intergenic 30.0%
TACAGAATAACTTCATGAGA+AGG - chr1_3:8148402-8148421 None:intergenic 30.0%
!! TTGCAAGTATTGATTGACTT+GGG - chr1_3:8148457-8148476 None:intergenic 30.0%
! TTTCATTGTTAATGACGGAG+CGG - chr1_3:8145014-8145033 None:intergenic 35.0%
! ACAGACGCTACTTTCATTAA+CGG + chr1_3:8145130-8145149 Msa0101500:CDS 35.0%
!! AACCGGTTTGGTTTAGAAAA+TGG - chr1_3:8145253-8145272 None:intergenic 35.0%
TTAACCTACATACACAGCAA+AGG + chr1_3:8145372-8145391 Msa0101500:CDS 35.0%
TAACCTACATACACAGCAAA+GGG + chr1_3:8145373-8145392 Msa0101500:CDS 35.0%
! AGGGGATAACAGAACAATTT+TGG + chr1_3:8145392-8145411 Msa0101500:CDS 35.0%
! CGAATTATGGTGTTGAATCT+GGG + chr1_3:8145454-8145473 Msa0101500:CDS 35.0%
GAAGTATAGCTATGTCCATA+AGG + chr1_3:8145488-8145507 Msa0101500:CDS 35.0%
CAATTGTAGTCAAACCCTTA+TGG - chr1_3:8145506-8145525 None:intergenic 35.0%
! TTATGGTGATGACTCATTGA+AGG + chr1_3:8145581-8145600 Msa0101500:CDS 35.0%
!! GAAACAAAGGGGTTGTTTTA+AGG + chr1_3:8145656-8145675 Msa0101500:CDS 35.0%
!!! ATGGTTTTGAATTAGGGTTG+AGG + chr1_3:8145741-8145760 Msa0101500:intron 35.0%
TGAATTAGGGTTGAGGAATT+TGG + chr1_3:8145748-8145767 Msa0101500:intron 35.0%
! GTAGAAAATTGTTGTTGACG+TGG + chr1_3:8145798-8145817 Msa0101500:intron 35.0%
TTGTGGTTGAAATATGGTTG+TGG + chr1_3:8145827-8145846 Msa0101500:intron 35.0%
!! AGCTGCTTAGAATTGATTTG+TGG + chr1_3:8146079-8146098 Msa0101500:intron 35.0%
CAGAAGAAAGACAAAAGACA+TGG - chr1_3:8146108-8146127 None:intergenic 35.0%
! AACTAATTTGGAGTAATCCC+GGG - chr1_3:8146250-8146269 None:intergenic 35.0%
! ATGGCTATGTTTGAGATTGA+GGG + chr1_3:8146311-8146330 Msa0101500:intron 35.0%
! TTTGAGGAGGTACAAAACAA+CGG + chr1_3:8146572-8146591 Msa0101500:intron 35.0%
TCAATTTCCAACTTCTTTGG+TGG + chr1_3:8146615-8146634 Msa0101500:intron 35.0%
ATTCATACCACCAAAGAAGT+TGG - chr1_3:8146625-8146644 None:intergenic 35.0%
CAAGACTTCCACTAAAGTAT+TGG - chr1_3:8146769-8146788 None:intergenic 35.0%
AACATAATTCGCTGGGTTTA+TGG + chr1_3:8146797-8146816 Msa0101500:intron 35.0%
ACATAATTCGCTGGGTTTAT+GGG + chr1_3:8146798-8146817 Msa0101500:intron 35.0%
GGTTGAAGAAGATAGATGAT+TGG + chr1_3:8147185-8147204 Msa0101500:intron 35.0%
! AAAACCCAACTTAATGGATC+GGG - chr1_3:8147229-8147248 None:intergenic 35.0%
TTTGTTCGAAAGACTCGTAT+AGG + chr1_3:8147531-8147550 Msa0101500:intron 35.0%
TTGTTCGAAAGACTCGTATA+GGG + chr1_3:8147532-8147551 Msa0101500:intron 35.0%
ACACAATTCGCAATTCGTTA+TGG - chr1_3:8147794-8147813 None:intergenic 35.0%
AATTGCGAATTGTGTGACTA+TGG + chr1_3:8147799-8147818 Msa0101500:intron 35.0%
TTCAAAATTCCATGTGGTCT+CGG - chr1_3:8147940-8147959 None:intergenic 35.0%
! GTTTGATATCTAGTTTGGGA+TGG + chr1_3:8147976-8147995 Msa0101500:three_prime_UTR 35.0%
AAATGGCAAAATATGCCTCA+TGG - chr1_3:8148014-8148033 None:intergenic 35.0%
TCATGTAATGAACTACTCTG+TGG + chr1_3:8148062-8148081 Msa0101500:three_prime_UTR 35.0%
GAGGGATGCATGAATAATAT+TGG - chr1_3:8148229-8148248 None:intergenic 35.0%
! ATCCTCTTTTCTTATGAGGA+GGG - chr1_3:8148247-8148266 None:intergenic 35.0%
! TATCCTCTTTTCTTATGAGG+AGG - chr1_3:8148248-8148267 None:intergenic 35.0%
TCCTCATAAGAAAAGAGGAT+AGG + chr1_3:8148247-8148266 Msa0101500:three_prime_UTR 35.0%
! TCCTATCCTCTTTTCTTATG+AGG - chr1_3:8148251-8148270 None:intergenic 35.0%
CAGAAAGAAAAAACTCCTAC+AGG + chr1_3:8148326-8148345 Msa0101500:three_prime_UTR 35.0%
!!! CCTAGCATGTTTTAGAGAAA+AGG - chr1_3:8148428-8148447 None:intergenic 35.0%
!!! CCTTTTCTCTAAAACATGCT+AGG + chr1_3:8148425-8148444 Msa0101500:three_prime_UTR 35.0%
!! CTTGCAAGTATTGATTGACT+TGG - chr1_3:8148458-8148477 None:intergenic 35.0%
GCGGCTTTCATTGTTAATGA+CGG - chr1_3:8145019-8145038 None:intergenic 40.0%
AACAATGAAAGCCGCTGTTA+AGG + chr1_3:8145024-8145043 Msa0101500:exon 40.0%
ACAATGAAAGCCGCTGTTAA+GGG + chr1_3:8145025-8145044 Msa0101500:exon 40.0%
AACCTACATACACAGCAAAG+GGG + chr1_3:8145374-8145393 Msa0101500:CDS 40.0%
! TTTTGGACGGAACATTTGTG+TGG + chr1_3:8145409-8145428 Msa0101500:CDS 40.0%
TTTGGACGGAACATTTGTGT+GGG + chr1_3:8145410-8145429 Msa0101500:CDS 40.0%
TCGCTGAAACCTTATTCGAT+GGG - chr1_3:8145437-8145456 None:intergenic 40.0%
TTCGCTGAAACCTTATTCGA+TGG - chr1_3:8145438-8145457 None:intergenic 40.0%
! GCGAATTATGGTGTTGAATC+TGG + chr1_3:8145453-8145472 Msa0101500:CDS 40.0%
ATGATGTTGCTAAGAATGCG+TGG + chr1_3:8145535-8145554 Msa0101500:CDS 40.0%
TGATGTTGCTAAGAATGCGT+GGG + chr1_3:8145536-8145555 Msa0101500:CDS 40.0%
! GCTGTTTCGAGGAAAGTTTA+TGG + chr1_3:8145564-8145583 Msa0101500:CDS 40.0%
AGACCACGAGTAAGAAGTTA+GGG + chr1_3:8145613-8145632 Msa0101500:CDS 40.0%
AAACCCTAACTTCTTACTCG+TGG - chr1_3:8145619-8145638 None:intergenic 40.0%
GTAAGAAGTTAGGGTTTGAG+TGG + chr1_3:8145622-8145641 Msa0101500:CDS 40.0%
GTCGAGGAATTCGAAACAAA+GGG + chr1_3:8145644-8145663 Msa0101500:CDS 40.0%
TCGAGGAATTCGAAACAAAG+GGG + chr1_3:8145645-8145664 Msa0101500:CDS 40.0%
TGTGGTACAGCTTGATGTTT+CGG + chr1_3:8145845-8145864 Msa0101500:intron 40.0%
TGATGTTTCGGCGACTATAA+TGG + chr1_3:8145857-8145876 Msa0101500:intron 40.0%
GCTGCATGTGATTACAATTC+TGG + chr1_3:8145962-8145981 Msa0101500:intron 40.0%
GCATCAGTGACTTAGAACAT+TGG + chr1_3:8146045-8146064 Msa0101500:intron 40.0%
! GATGGCTATGTTTGAGATTG+AGG + chr1_3:8146310-8146329 Msa0101500:intron 40.0%
TAAAGTAAAACGTCCTCCCT+CGG - chr1_3:8146488-8146507 None:intergenic 40.0%
GAAGAAATTGGTTGGGTTTG+AGG + chr1_3:8146556-8146575 Msa0101500:intron 40.0%
! TTGAGGAGGTACAAAACAAC+GGG + chr1_3:8146573-8146592 Msa0101500:intron 40.0%
AGGTACAAAACAACGGGATT+TGG + chr1_3:8146579-8146598 Msa0101500:intron 40.0%
GGTACAAAACAACGGGATTT+GGG + chr1_3:8146580-8146599 Msa0101500:intron 40.0%
GTACAAAACAACGGGATTTG+GGG + chr1_3:8146581-8146600 Msa0101500:intron 40.0%
AGCAGAGTAACATAATTCGC+TGG + chr1_3:8146789-8146808 Msa0101500:intron 40.0%
GCAGAGTAACATAATTCGCT+GGG + chr1_3:8146790-8146809 Msa0101500:intron 40.0%
!!! TCTTTTGCGTATTGATTCGC+TGG + chr1_3:8146858-8146877 Msa0101500:intron 40.0%
TTGAGACTGTTCAACCGTAA+AGG + chr1_3:8147273-8147292 Msa0101500:intron 40.0%
!! AGTTTTTGGGAAGTGAACAC+TGG + chr1_3:8147508-8147527 Msa0101500:intron 40.0%
TGAGAAGAATTCGGTACGTA+CGG - chr1_3:8147736-8147755 None:intergenic 40.0%
TACGCGACATGAGAAGAATT+CGG - chr1_3:8147745-8147764 None:intergenic 40.0%
! CTTGGGAATTTTCTTCTCCT+CGG - chr1_3:8147910-8147929 None:intergenic 40.0%
ATCCCTCCTCATAAGAAAAG+AGG + chr1_3:8148242-8148261 Msa0101500:three_prime_UTR 40.0%
GTCGTATTTGCCCTTAACAG+CGG - chr1_3:8145038-8145057 None:intergenic 45.0%
TAGCGTCTGTTATCGATGCT+CGG - chr1_3:8145120-8145139 None:intergenic 45.0%
ATTAACGGCCCAAGCTTAAC+CGG + chr1_3:8145145-8145164 Msa0101500:CDS 45.0%
! GAAGGAACCGGGTTTTTCTA+CGG - chr1_3:8145182-8145201 None:intergenic 45.0%
TAGTCGATACTGAAGGAACC+GGG - chr1_3:8145193-8145212 None:intergenic 45.0%
GTAGTCGATACTGAAGGAAC+CGG - chr1_3:8145194-8145213 None:intergenic 45.0%
GACGTTGTAGTCGATACTGA+AGG - chr1_3:8145200-8145219 None:intergenic 45.0%
! ATCCCCTTTGCTGTGTATGT+AGG - chr1_3:8145379-8145398 None:intergenic 45.0%
CAGACCACGAGTAAGAAGTT+AGG + chr1_3:8145612-8145631 Msa0101500:CDS 45.0%
GGTCGAGGAATTCGAAACAA+AGG + chr1_3:8145643-8145662 Msa0101500:CDS 45.0%
TGTTGACGTGGTTGCAATTG+TGG + chr1_3:8145810-8145829 Msa0101500:intron 45.0%
CCGGGAGAAAAACAAGTACA+CGG - chr1_3:8146232-8146251 None:intergenic 45.0%
!! CCGTGTACTTGTTTTTCTCC+CGG + chr1_3:8146229-8146248 Msa0101500:intron 45.0%
!! CGTGTACTTGTTTTTCTCCC+GGG + chr1_3:8146230-8146249 Msa0101500:intron 45.0%
TATGAACAGAGAATCCTCCG+AGG + chr1_3:8146468-8146487 Msa0101500:intron 45.0%
ATGAACAGAGAATCCTCCGA+GGG + chr1_3:8146469-8146488 Msa0101500:intron 45.0%
GAAATTGGTTGGGTTTGAGG+AGG + chr1_3:8146559-8146578 Msa0101500:intron 45.0%
TTTATGGGAGAACCGCGAAT+TGG + chr1_3:8146813-8146832 Msa0101500:intron 45.0%
TACGCAAAAGAACCGCGAAT+CGG - chr1_3:8146849-8146868 None:intergenic 45.0%
TTCATGGAATCGCCGTACTT+CGG - chr1_3:8146916-8146935 None:intergenic 45.0%
CGATTCCATGAACGTGTTTG+AGG + chr1_3:8146924-8146943 Msa0101500:intron 45.0%
ATGGTCCTCAAACACGTTCA+TGG - chr1_3:8146932-8146951 None:intergenic 45.0%
TGGATCGGGTGCAAACATAT+GGG - chr1_3:8147215-8147234 None:intergenic 45.0%
ATGGATCGGGTGCAAACATA+TGG - chr1_3:8147216-8147235 None:intergenic 45.0%
TTGCACCCGATCCATTAAGT+TGG + chr1_3:8147221-8147240 Msa0101500:intron 45.0%
TGCACCCGATCCATTAAGTT+GGG + chr1_3:8147222-8147241 Msa0101500:intron 45.0%
AGAAGAATTCGGTACGTACG+GGG - chr1_3:8147734-8147753 None:intergenic 45.0%
GAGAAGAATTCGGTACGTAC+GGG - chr1_3:8147735-8147754 None:intergenic 45.0%
ATTCGTTATGGTACGCGGTA+CGG - chr1_3:8147782-8147801 None:intergenic 45.0%
TCGCAATTCGTTATGGTACG+CGG - chr1_3:8147787-8147806 None:intergenic 45.0%
CTATCAGCTATGTCTCTTGC+AGG + chr1_3:8147863-8147882 Msa0101500:intron 45.0%
!! CTAGTTTGGGATGGCTTTTG+TGG + chr1_3:8147985-8148004 Msa0101500:three_prime_UTR 45.0%
GCGCAACAGCAAAAAAAAGC+AGG + chr1_3:8148271-8148290 Msa0101500:three_prime_UTR 45.0%
!!! TATTTATTTTTGTTTAATTT+TGG + chr1_3:8145290-8145309 Msa0101500:intron 5.0%
!!! TCATTTATTTTTATTTTTAT+TGG - chr1_3:8147478-8147497 None:intergenic 5.0%
!! GAGTTGGTGGTGAAAATCGG+AGG - chr1_3:8144992-8145011 None:intergenic 50.0%
!! GCGGAGTTGGTGGTGAAAAT+CGG - chr1_3:8144995-8145014 None:intergenic 50.0%
! TGTTAATGACGGAGCGGAGT+TGG - chr1_3:8145008-8145027 None:intergenic 50.0%
GGACTAAGCCGGTTAAGCTT+GGG - chr1_3:8145156-8145175 None:intergenic 50.0%
AGGACTAAGCCGGTTAAGCT+TGG - chr1_3:8145157-8145176 None:intergenic 50.0%
CGACTACAACGTCCCCAAAA+AGG + chr1_3:8145207-8145226 Msa0101500:CDS 50.0%
TGTGTGGGACCCATCGAATA+AGG + chr1_3:8145425-8145444 Msa0101500:CDS 50.0%
!!! CGTGGGATTTTGCTGTTTCG+AGG + chr1_3:8145553-8145572 Msa0101500:CDS 50.0%
AGTTAGGGTTTGAGTGGTCG+AGG + chr1_3:8145628-8145647 Msa0101500:CDS 50.0%
TGATTCGCTGGAATTCGTGC+CGG + chr1_3:8146870-8146889 Msa0101500:intron 50.0%
CAACTCAGCGAACCGAAGTA+CGG + chr1_3:8146901-8146920 Msa0101500:intron 50.0%
! CCATGTGCTGTGTTTTCCTG+CGG + chr1_3:8147143-8147162 Msa0101500:intron 50.0%
! GGCTGCACTTCTGTTTTGCT+AGG + chr1_3:8147164-8147183 Msa0101500:intron 50.0%
ATCGGGTGCAAACATATGGG+TGG - chr1_3:8147212-8147231 None:intergenic 50.0%
GAAGAATTCGGTACGTACGG+GGG - chr1_3:8147733-8147752 None:intergenic 50.0%
TTCGTTATGGTACGCGGTAC+GGG - chr1_3:8147781-8147800 None:intergenic 50.0%
GCTATGTCTCTTGCAGGTTG+TGG + chr1_3:8147869-8147888 Msa0101500:intron 50.0%
CTTCTCCTCGGCCAAGTTAA+CGG - chr1_3:8147898-8147917 None:intergenic 50.0%
! TGGCTTTTGTGGTCACCATG+AGG + chr1_3:8147996-8148015 Msa0101500:three_prime_UTR 50.0%
! TTGAGTTCCTGCTGTTTGCG+CGG - chr1_3:8148353-8148372 None:intergenic 50.0%
! TAATGACGGAGCGGAGTTGG+TGG - chr1_3:8145005-8145024 None:intergenic 55.0%
! TTTGACATCGCCGGCGTTGA+AGG - chr1_3:8145095-8145114 None:intergenic 55.0%
TGCTCGGAGTTTGACATCGC+CGG - chr1_3:8145104-8145123 None:intergenic 55.0%
TTCTACGGCGAGGACTAAGC+CGG - chr1_3:8145167-8145186 None:intergenic 55.0%
GTCCTCGCCGTAGAAAAACC+CGG + chr1_3:8145172-8145191 Msa0101500:CDS 55.0%
! AACCGGGTTTTTCTACGGCG+AGG - chr1_3:8145177-8145196 None:intergenic 55.0%
AACAGAGAATCCTCCGAGGG+AGG + chr1_3:8146472-8146491 Msa0101500:intron 55.0%
AGTAAAACGTCCTCCCTCGG+AGG - chr1_3:8146485-8146504 None:intergenic 55.0%
TCGCTGAGTTGCGCGAATTC+CGG - chr1_3:8146892-8146911 None:intergenic 55.0%
CCGCAGGAAAACACAGCACA+TGG - chr1_3:8147146-8147165 None:intergenic 55.0%
! CAAAACAGAAGTGCAGCCGC+AGG - chr1_3:8147162-8147181 None:intergenic 55.0%
ATTCGGTACGTACGGGGGTA+TGG - chr1_3:8147728-8147747 None:intergenic 55.0%
TATGGTACGCGGTACGGGTA+CGG - chr1_3:8147776-8147795 None:intergenic 55.0%
GGTTGTGGCGTCCGTTAACT+TGG + chr1_3:8147884-8147903 Msa0101500:intron 55.0%
GTGGTCTCGGCACTAAGCTT+GGG - chr1_3:8147927-8147946 None:intergenic 55.0%
TGTGGTCTCGGCACTAAGCT+TGG - chr1_3:8147928-8147947 None:intergenic 55.0%
! AGCTTAGTGCCGAGACCACA+TGG + chr1_3:8147928-8147947 Msa0101500:CDS 55.0%
CTTCCCCAACAGCAACACAG+AGG + chr1_3:8148294-8148313 Msa0101500:three_prime_UTR 55.0%
! GCTCCTCTGTGTTGCTGTTG+GGG - chr1_3:8148300-8148319 None:intergenic 55.0%
! GGCTCCTCTGTGTTGCTGTT+GGG - chr1_3:8148301-8148320 None:intergenic 55.0%
! TGAGTTCCTGCTGTTTGCGC+GGG - chr1_3:8148352-8148371 None:intergenic 55.0%
GGTACGCGCGAACCAATTCG+CGG - chr1_3:8146828-8146847 None:intergenic 60.0%
! CGTACGGGGGTATGGAGCTT+CGG - chr1_3:8147720-8147739 None:intergenic 60.0%
ATGGTACGCGGTACGGGTAC+GGG - chr1_3:8147775-8147794 None:intergenic 60.0%
! CGGCTCCTCTGTGTTGCTGT+TGG - chr1_3:8148302-8148321 None:intergenic 60.0%
GTTGAAGGCGACGGAGGCTG+CGG - chr1_3:8145080-8145099 None:intergenic 65.0%
! ATCGCCGGCGTTGAAGGCGA+CGG - chr1_3:8145089-8145108 None:intergenic 65.0%
!! GGTTCGCGCGTACCGATTCG+CGG + chr1_3:8146834-8146853 Msa0101500:intron 65.0%
! GGCGTCCGTTAACTTGGCCG+AGG + chr1_3:8147890-8147909 Msa0101500:CDS 65.0%
! TGCTGTTTGCGCGGGCCTGT+AGG - chr1_3:8148344-8148363 None:intergenic 65.0%
TACAGGCCCGCGCAAACAGC+AGG + chr1_3:8148343-8148362 Msa0101500:three_prime_UTR 65.0%
GCCTCCGTCGCCTTCAACGC+CGG + chr1_3:8145082-8145101 Msa0101500:CDS 70.0%
GCGGTACGGGTACGGGTACG+CGG - chr1_3:8147768-8147787 None:intergenic 70.0%
GAAGGCGACGGAGGCTGCGG+CGG - chr1_3:8145077-8145096 None:intergenic 75.0%
! GCCGGCGTTGAAGGCGACGG+AGG - chr1_3:8145086-8145105 None:intergenic 75.0%
! GGCGACGGAGGCTGCGGCGG+CGG - chr1_3:8145074-8145093 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 8144970 8148552 8144970 ID=Msa0101500;Name=Msa0101500
chr1_3 mRNA 8144970 8148552 8144970 ID=Msa0101500-mRNA-1;Parent=Msa0101500;Name=Msa0101500-mRNA-1;_AED=0.02;_eAED=0.02;_QI=58|1|1|1|1|1|3|587|213
chr1_3 exon 8144970 8145228 8144970 ID=Msa0101500-mRNA-1:exon:3206;Parent=Msa0101500-mRNA-1
chr1_3 exon 8145318 8145677 8145318 ID=Msa0101500-mRNA-1:exon:3207;Parent=Msa0101500-mRNA-1
chr1_3 exon 8147885 8148552 8147885 ID=Msa0101500-mRNA-1:exon:3208;Parent=Msa0101500-mRNA-1
chr1_3 five_prime_UTR 8144970 8145027 8144970 ID=Msa0101500-mRNA-1:five_prime_utr;Parent=Msa0101500-mRNA-1
chr1_3 CDS 8145028 8145228 8145028 ID=Msa0101500-mRNA-1:cds;Parent=Msa0101500-mRNA-1
chr1_3 CDS 8145318 8145677 8145318 ID=Msa0101500-mRNA-1:cds;Parent=Msa0101500-mRNA-1
chr1_3 CDS 8147885 8147965 8147885 ID=Msa0101500-mRNA-1:cds;Parent=Msa0101500-mRNA-1
chr1_3 three_prime_UTR 8147966 8148552 8147966 ID=Msa0101500-mRNA-1:three_prime_utr;Parent=Msa0101500-mRNA-1
Gene Sequence

>Msa0101500

ATGAAAGCCGCTGTTAAGGGCAAATACGACAATGACAAAAACGCCGCCGCCGCAGCCTCCGTCGCCTTCAACGCCGGCGATGTCAAACTCCGAGCATCGATAACAGACGCTACTTTCATTAACGGCCCAAGCTTAACCGGCTTAGTCCTCGCCGTAGAAAAACCCGGTTCCTTCAGTATCGACTACAACGTCCCCAAAAAGGATTTTAGGTTTCAATTTATGAACACAGTTAGGGTTGCTGATAAACCGTTGAATTTAACCTACATACACAGCAAAGGGGATAACAGAACAATTTTGGACGGAACATTTGTGTGGGACCCATCGAATAAGGTTTCAGCGAATTATGGTGTTGAATCTGGGAATTGTAGATTGAAGTATAGCTATGTCCATAAGGGTTTGACTACAATTGAACCTAGTTATGATGTTGCTAAGAATGCGTGGGATTTTGCTGTTTCGAGGAAAGTTTATGGTGATGACTCATTGAAGGCGACGTATCAGACCACGAGTAAGAAGTTAGGGTTTGAGTGGTCGAGGAATTCGAAACAAAGGGGTTGTTTTAAGGTTGTGGCGTCCGTTAACTTGGCCGAGGAGAAGAAAATTCCCAAGCTTAGTGCCGAGACCACATGGAATTTTGAAATGTAG
Protein sequence

>Msa0101500

MKAAVKGKYDNDKNAAAAASVAFNAGDVKLRASITDATFINGPSLTGLVLAVEKPGSFSIDYNVPKKDFRFQFMNTVRVADKPLNLTYIHSKGDNRTILDGTFVWDPSNKVSANYGVESGNCRLKYSYVHKGLTTIEPSYDVAKNAWDFAVSRKVYGDDSLKATYQTTSKKLGFEWSRNSKQRGCFKVVASVNLAEEKKIPKLSAETTWNFEM*