AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0572550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572550 MtrunA17_Chr8g0387991 88.950 181 14 1 1 175 1 181 3.52e-114 322
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572550 AT1G64355.1 65.333 150 35 5 21 156 28 174 1.03e-57 179
Msa0572550 AT1G64355.3 67.778 90 22 3 32 117 43 129 1.60e-32 114
Msa0572550 AT1G64355.2 68.182 88 21 3 32 115 43 127 3.83e-31 110

Find 52 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGTAATTTATCATTAAT+TGG 0.227652 4_1:+75455213 Msa0572550:three_prime_UTR
TCGGATGTGCCTCCAAATTT+TGG 0.228216 4_1:+75455105 Msa0572550:three_prime_UTR
TGATCATTGCACATCAAAAT+TGG 0.269030 4_1:-75455246 None:intergenic
GGTGAGGTTAGGAGAAGTTT+TGG 0.272109 4_1:-75454423 None:intergenic
GTTGAAGGGTTCATAAGTTT+AGG 0.306773 4_1:-75454194 None:intergenic
CACTAACTTGTTCATTGTGT+TGG 0.314311 4_1:+75454947 Msa0572550:CDS
TTAAATGAAAATCACGTTGC+AGG 0.357937 4_1:-75454223 None:intergenic
ACTAACTTGTTCATTGTGTT+GGG 0.374119 4_1:+75454948 Msa0572550:CDS
TTGAAGGGTTCATAAGTTTA+GGG 0.376906 4_1:-75454193 None:intergenic
TTGACAGCTAAGGTGCATTA+TGG 0.386555 4_1:+75454867 Msa0572550:intron
TGCCCCTGGGTGTTGATCCA+TGG 0.395407 4_1:+75454309 Msa0572550:CDS
AATCAGAAAAGCTGGAAATT+CGG 0.416724 4_1:+75454983 Msa0572550:CDS
TCTCCTAACCTCACCCTCTT+CGG 0.418605 4_1:+75454431 Msa0572550:CDS
AAACCGAAGAGGGTGAGGTT+AGG 0.440423 4_1:-75454434 None:intergenic
GATCGTGTGTGGATTGCCCC+TGG 0.440978 4_1:+75454295 Msa0572550:CDS
TCTAATGTGGATTGGTTACA+TGG 0.443829 4_1:+75454900 Msa0572550:CDS
CTTCTTTGTCTAATGTGGAT+TGG 0.447286 4_1:+75454892 Msa0572550:CDS
ATGTGGATTGGTTACATGGT+GGG 0.450371 4_1:+75454904 Msa0572550:CDS
TCGAAGGAGGAGAAGAAACC+TGG 0.458784 4_1:+75455020 Msa0572550:CDS
TGGAACTTCTTTGTCTAATG+TGG 0.464776 4_1:+75454887 Msa0572550:CDS
AGAGAGGGAATCAGAAAAGC+TGG 0.466045 4_1:+75454975 Msa0572550:CDS
GCCCCTGGGTGTTGATCCAT+GGG 0.473465 4_1:+75454310 Msa0572550:CDS
CGTTGCAGGAAGGTCGTTGA+AGG 0.474175 4_1:-75454209 None:intergenic
TGGAGGCACATCCGAGTCAA+TGG 0.486922 4_1:-75455097 None:intergenic
ATTGTGTTGGGTTTGAGAGA+GGG 0.496509 4_1:+75454960 Msa0572550:CDS
AGCGATGCTCTGTGAATCAA+TGG 0.506212 4_1:-75454339 None:intergenic
TGTAATTTGTTCCATTGACT+CGG 0.518063 4_1:+75455086 Msa0572550:three_prime_UTR
GGAGAAGAAACCTGGGATGA+AGG 0.519084 4_1:+75455028 Msa0572550:CDS
CATTGTGTTGGGTTTGAGAG+AGG 0.529587 4_1:+75454959 Msa0572550:CDS
GTGCCCATGGATCAACACCC+AGG 0.532974 4_1:-75454313 None:intergenic
AAGTACAAGAAACCAATGTA+AGG 0.534146 4_1:-75454389 None:intergenic
CAAGTTAGTGAGAGTGAGAA+GGG 0.535305 4_1:-75454934 None:intergenic
TGTGGATTGGTTACATGGTG+GGG 0.539691 4_1:+75454905 Msa0572550:CDS
AGTAGAAACCGAAGAGGGTG+AGG 0.547358 4_1:-75454439 None:intergenic
GATTTACAGAGAGATAGAAC+AGG 0.552911 4_1:-75454170 None:intergenic
TGCCCATGGATCAACACCCA+GGG 0.560771 4_1:-75454312 None:intergenic
AGCAACTAGACCTTCATCCC+AGG 0.562545 4_1:-75455038 None:intergenic
AAATCACAGCCAAAATTTGG+AGG 0.572411 4_1:-75455114 None:intergenic
ATCGTGTGTGGATTGCCCCT+GGG 0.578639 4_1:+75454296 Msa0572550:CDS
GGAACAAATTACAATAACAC+TGG 0.586606 4_1:-75455076 None:intergenic
AATGTGGATTGGTTACATGG+TGG 0.588190 4_1:+75454903 Msa0572550:CDS
ATGAAAATCACGTTGCAGGA+AGG 0.588711 4_1:-75454219 None:intergenic
ACAAGTTAGTGAGAGTGAGA+AGG 0.592216 4_1:-75454935 None:intergenic
AAATTACAATAACACTGGCA+TGG 0.603957 4_1:-75455071 None:intergenic
TGAAGGGTTCATAAGTTTAG+GGG 0.609851 4_1:-75454192 None:intergenic
TTACAAACAAAACAATCGAG+AGG 0.616166 4_1:-75455175 None:intergenic
CGAAGGAGGAGAAGAAACCT+GGG 0.616213 4_1:+75455021 Msa0572550:CDS
AAGAGAACAGTGATCGTGTG+TGG 0.618841 4_1:+75454284 Msa0572550:CDS
GTTGCAGGAAGGTCGTTGAA+GGG 0.620276 4_1:-75454208 None:intergenic
GAATCAATGGAAGGTGCCCA+TGG 0.621299 4_1:-75454326 None:intergenic
GCCCATGGATCAACACCCAG+GGG 0.698475 4_1:-75454311 None:intergenic
ATGCTCTGTGAATCAATGGA+AGG 0.701730 4_1:-75454335 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAATAAAAAGGAAA+AGG - chr4_1:75454556-75454575 None:intergenic 10.0%
!!! AAAAAAATTAATTTGCAGAA+TGG + chr4_1:75454124-75454143 Msa0572550:exon 15.0%
!! TAATTTAATCATTTCAAATG+TGG - chr4_1:75454827-75454846 None:intergenic 15.0%
!! TTGTGTAATTTATCATTAAT+TGG + chr4_1:75455213-75455232 Msa0572550:three_prime_UTR 15.0%
!!! CTCTAATTGAACTTTATTTT+CGG - chr4_1:75454624-75454643 None:intergenic 20.0%
!!! TTTTCTTTGTTTCCTTACAT+TGG + chr4_1:75454377-75454396 Msa0572550:CDS 25.0%
! AAGAAAAGAAAACTAACTTG+TGG - chr4_1:75454486-75454505 None:intergenic 25.0%
! TTGATTTATTATGATTCAGC+TGG + chr4_1:75454756-75454775 Msa0572550:intron 25.0%
! TTTAATTGTTCTGTTTGGTT+CGG + chr4_1:75454779-75454798 Msa0572550:intron 25.0%
! ATTACTTATGTTTAGTTGTG+TGG + chr4_1:75454843-75454862 Msa0572550:intron 25.0%
! AAAAAATCACAGCCAAAATT+TGG - chr4_1:75455120-75455139 None:intergenic 25.0%
TTGAAGGGTTCATAAGTTTA+GGG - chr4_1:75454196-75454215 None:intergenic 30.0%
TTAAATGAAAATCACGTTGC+AGG - chr4_1:75454226-75454245 None:intergenic 30.0%
AAGTACAAGAAACCAATGTA+AGG - chr4_1:75454392-75454411 None:intergenic 30.0%
CTACGATTAAGTTATTCGAA+CGG - chr4_1:75454723-75454742 None:intergenic 30.0%
!!! TGGTTCGGTTTTTAGAATAT+TGG + chr4_1:75454794-75454813 Msa0572550:intron 30.0%
!!! GGTTCGGTTTTTAGAATATT+GGG + chr4_1:75454795-75454814 Msa0572550:intron 30.0%
!! ACTAACTTGTTCATTGTGTT+GGG + chr4_1:75454948-75454967 Msa0572550:CDS 30.0%
AATCAGAAAAGCTGGAAATT+CGG + chr4_1:75454983-75455002 Msa0572550:CDS 30.0%
AAATTACAATAACACTGGCA+TGG - chr4_1:75455074-75455093 None:intergenic 30.0%
GGAACAAATTACAATAACAC+TGG - chr4_1:75455079-75455098 None:intergenic 30.0%
!! TGTAATTTGTTCCATTGACT+CGG + chr4_1:75455086-75455105 Msa0572550:three_prime_UTR 30.0%
TTACAAACAAAACAATCGAG+AGG - chr4_1:75455178-75455197 None:intergenic 30.0%
TGATCATTGCACATCAAAAT+TGG - chr4_1:75455249-75455268 None:intergenic 30.0%
GATTTACAGAGAGATAGAAC+AGG - chr4_1:75454173-75454192 None:intergenic 35.0%
TGAAGGGTTCATAAGTTTAG+GGG - chr4_1:75454195-75454214 None:intergenic 35.0%
GTTGAAGGGTTCATAAGTTT+AGG - chr4_1:75454197-75454216 None:intergenic 35.0%
!!! GTTAGGAGAAGTTTTGGATT+TGG - chr4_1:75454420-75454439 None:intergenic 35.0%
CAAAAAGTAGAAACCGAAGA+GGG - chr4_1:75454447-75454466 None:intergenic 35.0%
!! TACTTTTTGCTCGCTTTTGT+TGG + chr4_1:75454458-75454477 Msa0572550:CDS 35.0%
!!! TTATTTTGAACAGTTCCAGC+TGG + chr4_1:75454572-75454591 Msa0572550:intron 35.0%
!!! CAATGTTTTAAAAGCCGAAC+TGG + chr4_1:75454652-75454671 Msa0572550:intron 35.0%
! GCTGGTTTAATTGTTCTGTT+TGG + chr4_1:75454774-75454793 Msa0572550:intron 35.0%
TGGAACTTCTTTGTCTAATG+TGG + chr4_1:75454887-75454906 Msa0572550:CDS 35.0%
CTTCTTTGTCTAATGTGGAT+TGG + chr4_1:75454892-75454911 Msa0572550:CDS 35.0%
!! TCTAATGTGGATTGGTTACA+TGG + chr4_1:75454900-75454919 Msa0572550:CDS 35.0%
!! CACTAACTTGTTCATTGTGT+TGG + chr4_1:75454947-75454966 Msa0572550:CDS 35.0%
TGGAAATTCGGAGAAAAACA+CGG + chr4_1:75454995-75455014 Msa0572550:CDS 35.0%
AAATCACAGCCAAAATTTGG+AGG - chr4_1:75455117-75455136 None:intergenic 35.0%
ATGAAAATCACGTTGCAGGA+AGG - chr4_1:75454222-75454241 None:intergenic 40.0%
ATGCTCTGTGAATCAATGGA+AGG - chr4_1:75454338-75454357 None:intergenic 40.0%
GCAAAAAGTAGAAACCGAAG+AGG - chr4_1:75454448-75454467 None:intergenic 40.0%
ACAGTTCCAGCTGGAATTTA+TGG + chr4_1:75454581-75454600 Msa0572550:intron 40.0%
ACAGCATACAGAATGACAGT+TGG + chr4_1:75454696-75454715 Msa0572550:intron 40.0%
TTGACAGCTAAGGTGCATTA+TGG + chr4_1:75454867-75454886 Msa0572550:intron 40.0%
!! AATGTGGATTGGTTACATGG+TGG + chr4_1:75454903-75454922 Msa0572550:CDS 40.0%
!! ATGTGGATTGGTTACATGGT+GGG + chr4_1:75454904-75454923 Msa0572550:CDS 40.0%
CAAGTTAGTGAGAGTGAGAA+GGG - chr4_1:75454937-75454956 None:intergenic 40.0%
ACAAGTTAGTGAGAGTGAGA+AGG - chr4_1:75454938-75454957 None:intergenic 40.0%
ATTGTGTTGGGTTTGAGAGA+GGG + chr4_1:75454960-75454979 Msa0572550:CDS 40.0%
AAGAGAACAGTGATCGTGTG+TGG + chr4_1:75454284-75454303 Msa0572550:CDS 45.0%
AGCGATGCTCTGTGAATCAA+TGG - chr4_1:75454342-75454361 None:intergenic 45.0%
! GGTGAGGTTAGGAGAAGTTT+TGG - chr4_1:75454426-75454445 None:intergenic 45.0%
GACTCACCATAAATTCCAGC+TGG - chr4_1:75454590-75454609 None:intergenic 45.0%
AGCCGAACTGGACATTAAAC+CGG + chr4_1:75454664-75454683 Msa0572550:intron 45.0%
!! TGCCGGTTTAATGTCCAGTT+CGG - chr4_1:75454669-75454688 None:intergenic 45.0%
AACTGGACATTAAACCGGCA+TGG + chr4_1:75454669-75454688 Msa0572550:intron 45.0%
TGTATGCTGTTGAACCATGC+CGG - chr4_1:75454686-75454705 None:intergenic 45.0%
!! TGTGGATTGGTTACATGGTG+GGG + chr4_1:75454905-75454924 Msa0572550:CDS 45.0%
CATTGTGTTGGGTTTGAGAG+AGG + chr4_1:75454959-75454978 Msa0572550:CDS 45.0%
AGAGAGGGAATCAGAAAAGC+TGG + chr4_1:75454975-75454994 Msa0572550:CDS 45.0%
GGAGAAAAACACGGAATCGA+AGG + chr4_1:75455004-75455023 Msa0572550:CDS 45.0%
GAAAAACACGGAATCGAAGG+AGG + chr4_1:75455007-75455026 Msa0572550:CDS 45.0%
! TCGGATGTGCCTCCAAATTT+TGG + chr4_1:75455105-75455124 Msa0572550:three_prime_UTR 45.0%
!! TTCAAAATAAATAAATAAAA+AGG - chr4_1:75454562-75454581 None:intergenic 5.0%
GTTGCAGGAAGGTCGTTGAA+GGG - chr4_1:75454211-75454230 None:intergenic 50.0%
!! GAATCAATGGAAGGTGCCCA+TGG - chr4_1:75454329-75454348 None:intergenic 50.0%
TCTCCTAACCTCACCCTCTT+CGG + chr4_1:75454431-75454450 Msa0572550:CDS 50.0%
AAACCGAAGAGGGTGAGGTT+AGG - chr4_1:75454437-75454456 None:intergenic 50.0%
AGTAGAAACCGAAGAGGGTG+AGG - chr4_1:75454442-75454461 None:intergenic 50.0%
GTTGTGTGGCTTGACAGCTA+AGG + chr4_1:75454857-75454876 Msa0572550:intron 50.0%
TCGAAGGAGGAGAAGAAACC+TGG + chr4_1:75455020-75455039 Msa0572550:CDS 50.0%
CGAAGGAGGAGAAGAAACCT+GGG + chr4_1:75455021-75455040 Msa0572550:CDS 50.0%
GGAGAAGAAACCTGGGATGA+AGG + chr4_1:75455028-75455047 Msa0572550:CDS 50.0%
AGCAACTAGACCTTCATCCC+AGG - chr4_1:75455041-75455060 None:intergenic 50.0%
CGTTGCAGGAAGGTCGTTGA+AGG - chr4_1:75454212-75454231 None:intergenic 55.0%
! ATCGTGTGTGGATTGCCCCT+GGG + chr4_1:75454296-75454315 Msa0572550:CDS 55.0%
TGCCCATGGATCAACACCCA+GGG - chr4_1:75454315-75454334 None:intergenic 55.0%
! TGGAGGCACATCCGAGTCAA+TGG - chr4_1:75455100-75455119 None:intergenic 55.0%
! GATCGTGTGTGGATTGCCCC+TGG + chr4_1:75454295-75454314 Msa0572550:CDS 60.0%
!! TGCCCCTGGGTGTTGATCCA+TGG + chr4_1:75454309-75454328 Msa0572550:CDS 60.0%
!! GCCCCTGGGTGTTGATCCAT+GGG + chr4_1:75454310-75454329 Msa0572550:CDS 60.0%
GCCCATGGATCAACACCCAG+GGG - chr4_1:75454314-75454333 None:intergenic 60.0%
GTGCCCATGGATCAACACCC+AGG - chr4_1:75454316-75454335 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 75454123 75455396 75454123 ID=Msa0572550;Name=Msa0572550
chr4_1 mRNA 75454123 75455396 75454123 ID=Msa0572550-mRNA-1;Parent=Msa0572550;Name=Msa0572550-mRNA-1;_AED=0.02;_eAED=0.02;_QI=20|0|1|1|1|1|2|341|175
chr4_1 exon 75454123 75454488 75454123 ID=Msa0572550-mRNA-1:exon:26108;Parent=Msa0572550-mRNA-1
chr4_1 exon 75454874 75455396 75454874 ID=Msa0572550-mRNA-1:exon:26109;Parent=Msa0572550-mRNA-1
chr4_1 five_prime_UTR 75454123 75454142 75454123 ID=Msa0572550-mRNA-1:five_prime_utr;Parent=Msa0572550-mRNA-1
chr4_1 CDS 75454143 75454488 75454143 ID=Msa0572550-mRNA-1:cds;Parent=Msa0572550-mRNA-1
chr4_1 CDS 75454874 75455055 75454874 ID=Msa0572550-mRNA-1:cds;Parent=Msa0572550-mRNA-1
chr4_1 three_prime_UTR 75455056 75455396 75455056 ID=Msa0572550-mRNA-1:three_prime_utr;Parent=Msa0572550-mRNA-1
Gene Sequence

>Msa0572550

ATGGCTTCTTCTGCTATAAACTTATTACCTGTTCTATCTCTCTGTAAATCCCCTAAACTTATGAACCCTTCAACGACCTTCCTGCAACGTGATTTTCATTTAAGCAGCGTCAGAACAATCAGAGAATCAAACAGCTTTAGAAAGAGAACAGTGATCGTGTGTGGATTGCCCCTGGGTGTTGATCCATGGGCACCTTCCATTGATTCACAGAGCATCGCTTCTCAACTATTTGCGTTTTCTTTGTTTCCTTACATTGGTTTCTTGTACTTCATTACCAAATCCAAAACTTCTCCTAACCTCACCCTCTTCGGTTTCTACTTTTTGCTCGCTTTTGTTGGTGCCACAACTAAGGTGCATTATGGAACTTCTTTGTCTAATGTGGATTGGTTACATGGTGGGGCTGAGTCCCTTCTCACTCTCACTAACTTGTTCATTGTGTTGGGTTTGAGAGAGGGAATCAGAAAAGCTGGAAATTCGGAGAAAAACACGGAATCGAAGGAGGAGAAGAAACCTGGGATGAAGGTCTAG

Protein sequence

>Msa0572550

MASSAINLLPVLSLCKSPKLMNPSTTFLQRDFHLSSVRTIRESNSFRKRTVIVCGLPLGVDPWAPSIDSQSIASQLFAFSLFPYIGFLYFITKSKTSPNLTLFGFYFLLAFVGATTKVHYGTSLSNVDWLHGGAESLLTLTNLFIVLGLREGIRKAGNSEKNTESKEEKKPGMKV*