AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393940 MtrunA17_Chr7g0273171 91.667 168 10 2 1 168 1 164 3.95e-105 298
Msa1393940 MtrunA17_Chr2g0288981 39.053 169 90 4 7 165 2 167 8.12e-32 112
Msa1393940 MtrunA17_Chr4g0025231 35.429 175 88 3 13 166 7 177 3.03e-30 108
Msa1393940 MtrunA17_Chr2g0304821 36.196 163 91 2 17 166 14 176 7.43e-26 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393940 AT1G05070.1 38.922 167 83 5 15 166 6 168 1.17e-32 115
Msa1393940 AT2G32580.1 37.423 163 90 3 15 166 6 167 3.24e-31 111
Msa1393940 AT4G04360.1 42.236 161 79 4 18 166 10 168 4.84e-29 105
Msa1393940 AT2G24290.1 40.373 161 81 3 21 166 12 172 6.89e-29 105
Msa1393940 AT4G30996.1 37.736 159 85 2 21 165 12 170 1.10e-27 102
Msa1393940 AT2G32580.2 43.000 100 57 0 67 166 6 105 3.15e-24 91.7

Find 52 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAACTAAATTAGTATTT+TGG 0.141693 tig0021765:-98708 Msa1393940:three_prime_UTR
GGAATATCCCGTTGCTTCTT+TGG 0.212587 tig0021765:-99830 Msa1393940:CDS
TGCATTTATTACTAATCTTT+TGG 0.212992 tig0021765:+99020 None:intergenic
GAAGAACGAGCTCGTGATTA+TGG 0.234749 tig0021765:-98871 Msa1393940:CDS
TTCATTTCCTCCTTCATTGT+TGG 0.237361 tig0021765:+99117 None:intergenic
AGCGCAGGTTGACGGCATTA+TGG 0.273292 tig0021765:-98894 Msa1393940:CDS
GTTCAATTGAGTGACAAATT+TGG 0.277581 tig0021765:-100048 Msa1393940:five_prime_UTR
GAGCAAAGACAATGTTCTTA+TGG 0.346328 tig0021765:-99988 Msa1393940:CDS
CTGTTCCTCACTTGCCTCAC+TGG 0.367272 tig0021765:-99851 Msa1393940:CDS
TGTTACCTTGTGTTCACAAT+TGG 0.371180 tig0021765:-99931 Msa1393940:CDS
GAATATCCCGTTGCTTCTTT+GGG 0.376906 tig0021765:-99829 Msa1393940:intron
GCGCAGGTTGACGGCATTAT+GGG 0.391190 tig0021765:-98893 Msa1393940:CDS
GTGACAAATTTGGTTTGGTT+TGG 0.404272 tig0021765:-100038 Msa1393940:five_prime_UTR
ATTGAGTGACAAATTTGGTT+TGG 0.407348 tig0021765:-100043 Msa1393940:five_prime_UTR
TTACTATATGTACAGATTGT+GGG 0.418392 tig0021765:-99149 Msa1393940:intron
AGTAGTGATAGTTTATTAGT+TGG 0.424734 tig0021765:+98806 None:intergenic
GAAGTAGAAAAGTGCAGCAT+AGG 0.426789 tig0021765:-98970 Msa1393940:CDS
CAGAGCTTGTTGAAGAGCGC+AGG 0.429709 tig0021765:-98909 Msa1393940:CDS
TTTGGTTTGGTTTGCAATGA+GGG 0.434025 tig0021765:-100025 Msa1393940:exon
AAGAACGAGCTCGTGATTAT+GGG 0.441049 tig0021765:-98870 Msa1393940:CDS
GGTCTGTTCCAAAGTCTCAT+TGG 0.443502 tig0021765:+99041 None:intergenic
TTTAAGACCCTGCCGTTAAG+TGG 0.447186 tig0021765:+98679 None:intergenic
AATTGTAGCCAATGAGACTT+TGG 0.447499 tig0021765:-99049 Msa1393940:CDS
GTGATTATGGGTGGAAGGAC+AGG 0.457853 tig0021765:-98858 Msa1393940:CDS
AACTTACCCAAAGAAGCAAC+GGG 0.499220 tig0021765:+99823 None:intergenic
TGTTGAAGAGCGCAGGTTGA+CGG 0.500440 tig0021765:-98902 Msa1393940:CDS
GGTGAATTCCACTTAACGGC+AGG 0.511432 tig0021765:-98687 Msa1393940:three_prime_UTR
AAGTAGAAAAGTGCAGCATA+GGG 0.518644 tig0021765:-98969 Msa1393940:CDS
TTGTGATTATTTAACCATGT+AGG 0.521426 tig0021765:-98738 Msa1393940:three_prime_UTR
GTTTGGTTTGGTTTGCAATG+AGG 0.525335 tig0021765:-100026 Msa1393940:exon
TTCTTCAAACGCCAGCGAGA+TGG 0.541960 tig0021765:+99904 None:intergenic
AGAAAAGTGCAGCATAGGGA+TGG 0.556983 tig0021765:-98965 Msa1393940:CDS
CAATCATGACATTGAGGACA+AGG 0.561176 tig0021765:+99877 None:intergenic
CAATTGGACCACCATCTCGC+TGG 0.563845 tig0021765:-99915 Msa1393940:CDS
TGGAAACTTGTGAAGAAGCA+AGG 0.573605 tig0021765:-98945 Msa1393940:CDS
TTCAAACGCCAGCGAGATGG+TGG 0.580007 tig0021765:+99907 None:intergenic
AGAAGCAAGGGAGAGAGCAG+AGG 0.585238 tig0021765:-98932 Msa1393940:CDS
GAGACATGATCCAACAATGA+AGG 0.590741 tig0021765:-99127 Msa1393940:CDS
AGCTCGTGATTATGGGTGGA+AGG 0.596678 tig0021765:-98863 Msa1393940:CDS
AAAGATTAGTAATAAATGCA+AGG 0.596837 tig0021765:-99017 Msa1393940:CDS
TCCTCTGATAGCATTGTCAG+TGG 0.601328 tig0021765:+99087 None:intergenic
AAACTTACCCAAAGAAGCAA+CGG 0.604283 tig0021765:+99822 None:intergenic
AACGAGCTCGTGATTATGGG+TGG 0.612321 tig0021765:-98867 Msa1393940:CDS
GTGAATTCCACTTAACGGCA+GGG 0.613965 tig0021765:-98686 Msa1393940:three_prime_UTR
GGAAACTTGTGAAGAAGCAA+GGG 0.615984 tig0021765:-98944 Msa1393940:CDS
CAGTAACAATCATGACATTG+AGG 0.634830 tig0021765:+99871 None:intergenic
TCCACTGACAATGCTATCAG+AGG 0.642154 tig0021765:-99088 Msa1393940:CDS
ATATTCCAGTGAGGCAAGTG+AGG 0.642772 tig0021765:+99846 None:intergenic
ACATGATCCAACAATGAAGG+AGG 0.647322 tig0021765:-99124 Msa1393940:CDS
GTGGTCCAATTGTGAACACA+AGG 0.658093 tig0021765:+99926 None:intergenic
AATGAAGGAGGAAATGAACA+AGG 0.667487 tig0021765:-99112 Msa1393940:CDS
AGCAACGGGATATTCCAGTG+AGG 0.784097 tig0021765:+99837 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAAATTTTACTTAAAG+TGG + tig0021765:99474-99493 None:intergenic 10.0%
!!! AAAATACTAATTTAGTTTTA+AGG + tig0021765:100058-100077 None:intergenic 10.0%
!!! TTAAAACTAAATTAGTATTT+TGG - tig0021765:100057-100076 Msa1393940:five_prime_UTR 10.0%
!!! ATTATAAATTTTACTTAAAG+TGG + tig0021765:99474-99493 None:intergenic 10.0%
!!! AAAATACTAATTTAGTTTTA+AGG + tig0021765:100058-100077 None:intergenic 10.0%
!!! TTAAAACTAAATTAGTATTT+TGG - tig0021765:100057-100076 Msa1393940:five_prime_UTR 10.0%
!! TAGTAATTAGAAAAATTCAC+TGG - tig0021765:99078-99097 Msa1393940:CDS 20.0%
!! CAATTCAATTCATATGTATT+AGG - tig0021765:99165-99184 Msa1393940:intron 20.0%
!! TAGGTTTATATATTAATTCG+TGG - tig0021765:99184-99203 Msa1393940:intron 20.0%
!! AGAAATGCAAAACATTTAAA+TGG + tig0021765:99385-99404 None:intergenic 20.0%
!!! TGCATTTATTACTAATCTTT+TGG + tig0021765:99748-99767 None:intergenic 20.0%
!! AAAGATTAGTAATAAATGCA+AGG - tig0021765:99748-99767 Msa1393940:intron 20.0%
!! TAGTAATTAGAAAAATTCAC+TGG - tig0021765:99078-99097 Msa1393940:CDS 20.0%
!! CAATTCAATTCATATGTATT+AGG - tig0021765:99165-99184 Msa1393940:intron 20.0%
!! TAGGTTTATATATTAATTCG+TGG - tig0021765:99184-99203 Msa1393940:intron 20.0%
!! AGAAATGCAAAACATTTAAA+TGG + tig0021765:99385-99404 None:intergenic 20.0%
!!! TGCATTTATTACTAATCTTT+TGG + tig0021765:99748-99767 None:intergenic 20.0%
!! AAAGATTAGTAATAAATGCA+AGG - tig0021765:99748-99767 Msa1393940:intron 20.0%
! TTTGTAGAGTAAGTGAAAAA+GGG + tig0021765:98969-98988 None:intergenic 25.0%
! ATTTGTAGAGTAAGTGAAAA+AGG + tig0021765:98970-98989 None:intergenic 25.0%
!!! CATCATTTTTCAAACACAAA+GGG + tig0021765:99055-99074 None:intergenic 25.0%
! ATTGAATAACCAAAACATTC+TGG + tig0021765:99149-99168 None:intergenic 25.0%
! TTTACTATATGTACAGATTG+TGG - tig0021765:99615-99634 Msa1393940:intron 25.0%
! TTACTATATGTACAGATTGT+GGG - tig0021765:99616-99635 Msa1393940:intron 25.0%
! AGTAGTGATAGTTTATTAGT+TGG + tig0021765:99962-99981 None:intergenic 25.0%
! TTGTGATTATTTAACCATGT+AGG - tig0021765:100027-100046 Msa1393940:exon 25.0%
! TTTGTAGAGTAAGTGAAAAA+GGG + tig0021765:98969-98988 None:intergenic 25.0%
! ATTTGTAGAGTAAGTGAAAA+AGG + tig0021765:98970-98989 None:intergenic 25.0%
!!! CATCATTTTTCAAACACAAA+GGG + tig0021765:99055-99074 None:intergenic 25.0%
! ATTGAATAACCAAAACATTC+TGG + tig0021765:99149-99168 None:intergenic 25.0%
! TTTACTATATGTACAGATTG+TGG - tig0021765:99615-99634 Msa1393940:intron 25.0%
! TTACTATATGTACAGATTGT+GGG - tig0021765:99616-99635 Msa1393940:intron 25.0%
! AGTAGTGATAGTTTATTAGT+TGG + tig0021765:99962-99981 None:intergenic 25.0%
! TTGTGATTATTTAACCATGT+AGG - tig0021765:100027-100046 Msa1393940:exon 25.0%
GTTCAATTGAGTGACAAATT+TGG - tig0021765:98717-98736 Msa1393940:three_prime_UTR 30.0%
ATTGAGTGACAAATTTGGTT+TGG - tig0021765:98722-98741 Msa1393940:three_prime_UTR 30.0%
!!! TTCTTATGGTGTTTTGAAGT+GGG - tig0021765:98791-98810 Msa1393940:three_prime_UTR 30.0%
ACTCTACAAATTAACAGACT+TGG - tig0021765:98979-98998 Msa1393940:CDS 30.0%
ACAAGTTTGATTAGATGTAC+AGG - tig0021765:99018-99037 Msa1393940:CDS 30.0%
!! GCATCATTTTTCAAACACAA+AGG + tig0021765:99056-99075 None:intergenic 30.0%
CTTTGTTCTGATTCTCAAAA+AGG + tig0021765:99107-99126 None:intergenic 30.0%
! ATGTATGTTTTGTATCACAG+TGG + tig0021765:99275-99294 None:intergenic 30.0%
TGATACAAAACATACATCGA+AGG - tig0021765:99278-99297 Msa1393940:intron 30.0%
CTAAAAATGAGAACAGAATG+AGG + tig0021765:99534-99553 None:intergenic 30.0%
ATAATGTCCAAAAGACAACA+TGG + tig0021765:99577-99596 None:intergenic 30.0%
! TTTTGGTGAATTCCACTTAA+CGG - tig0021765:100074-100093 Msa1393940:five_prime_UTR 30.0%
GTTCAATTGAGTGACAAATT+TGG - tig0021765:98717-98736 Msa1393940:three_prime_UTR 30.0%
ATTGAGTGACAAATTTGGTT+TGG - tig0021765:98722-98741 Msa1393940:three_prime_UTR 30.0%
!!! TTCTTATGGTGTTTTGAAGT+GGG - tig0021765:98791-98810 Msa1393940:three_prime_UTR 30.0%
ACTCTACAAATTAACAGACT+TGG - tig0021765:98979-98998 Msa1393940:CDS 30.0%
ACAAGTTTGATTAGATGTAC+AGG - tig0021765:99018-99037 Msa1393940:CDS 30.0%
!! GCATCATTTTTCAAACACAA+AGG + tig0021765:99056-99075 None:intergenic 30.0%
CTTTGTTCTGATTCTCAAAA+AGG + tig0021765:99107-99126 None:intergenic 30.0%
! ATGTATGTTTTGTATCACAG+TGG + tig0021765:99275-99294 None:intergenic 30.0%
TGATACAAAACATACATCGA+AGG - tig0021765:99278-99297 Msa1393940:intron 30.0%
CTAAAAATGAGAACAGAATG+AGG + tig0021765:99534-99553 None:intergenic 30.0%
ATAATGTCCAAAAGACAACA+TGG + tig0021765:99577-99596 None:intergenic 30.0%
! TTTTGGTGAATTCCACTTAA+CGG - tig0021765:100074-100093 Msa1393940:five_prime_UTR 30.0%
!! GTGACAAATTTGGTTTGGTT+TGG - tig0021765:98727-98746 Msa1393940:three_prime_UTR 35.0%
!! TTTGGTTTGGTTTGCAATGA+GGG - tig0021765:98740-98759 Msa1393940:three_prime_UTR 35.0%
GAGCAAAGACAATGTTCTTA+TGG - tig0021765:98777-98796 Msa1393940:three_prime_UTR 35.0%
!!! GTTCTTATGGTGTTTTGAAG+TGG - tig0021765:98790-98809 Msa1393940:three_prime_UTR 35.0%
!!! TTTTGAAGTGGGTTTTGGTT+TGG - tig0021765:98802-98821 Msa1393940:three_prime_UTR 35.0%
TGTTACCTTGTGTTCACAAT+TGG - tig0021765:98834-98853 Msa1393940:exon 35.0%
CAGTAACAATCATGACATTG+AGG + tig0021765:98897-98916 None:intergenic 35.0%
AAACTTACCCAAAGAAGCAA+CGG + tig0021765:98946-98965 None:intergenic 35.0%
! TCTATCTAGCCAGAATGTTT+TGG - tig0021765:99137-99156 Msa1393940:CDS 35.0%
!! CATAGCTAGCTTTGTTTATG+TGG - tig0021765:99410-99429 Msa1393940:intron 35.0%
TTCATTTCCTCCTTCATTGT+TGG + tig0021765:99651-99670 None:intergenic 35.0%
AATGAAGGAGGAAATGAACA+AGG - tig0021765:99653-99672 Msa1393940:intron 35.0%
AATTGTAGCCAATGAGACTT+TGG - tig0021765:99716-99735 Msa1393940:intron 35.0%
! AAGTAGAAAAGTGCAGCATA+GGG - tig0021765:99796-99815 Msa1393940:intron 35.0%
!! GTGACAAATTTGGTTTGGTT+TGG - tig0021765:98727-98746 Msa1393940:three_prime_UTR 35.0%
!! TTTGGTTTGGTTTGCAATGA+GGG - tig0021765:98740-98759 Msa1393940:three_prime_UTR 35.0%
GAGCAAAGACAATGTTCTTA+TGG - tig0021765:98777-98796 Msa1393940:three_prime_UTR 35.0%
!!! GTTCTTATGGTGTTTTGAAG+TGG - tig0021765:98790-98809 Msa1393940:three_prime_UTR 35.0%
!!! TTTTGAAGTGGGTTTTGGTT+TGG - tig0021765:98802-98821 Msa1393940:three_prime_UTR 35.0%
TGTTACCTTGTGTTCACAAT+TGG - tig0021765:98834-98853 Msa1393940:exon 35.0%
CAGTAACAATCATGACATTG+AGG + tig0021765:98897-98916 None:intergenic 35.0%
AAACTTACCCAAAGAAGCAA+CGG + tig0021765:98946-98965 None:intergenic 35.0%
! TCTATCTAGCCAGAATGTTT+TGG - tig0021765:99137-99156 Msa1393940:CDS 35.0%
!! CATAGCTAGCTTTGTTTATG+TGG - tig0021765:99410-99429 Msa1393940:intron 35.0%
TTCATTTCCTCCTTCATTGT+TGG + tig0021765:99651-99670 None:intergenic 35.0%
AATGAAGGAGGAAATGAACA+AGG - tig0021765:99653-99672 Msa1393940:intron 35.0%
AATTGTAGCCAATGAGACTT+TGG - tig0021765:99716-99735 Msa1393940:intron 35.0%
! AAGTAGAAAAGTGCAGCATA+GGG - tig0021765:99796-99815 Msa1393940:intron 35.0%
!! GTTTGGTTTGGTTTGCAATG+AGG - tig0021765:98739-98758 Msa1393940:three_prime_UTR 40.0%
!!! TGGTGTTTTGAAGTGGGTTT+TGG - tig0021765:98797-98816 Msa1393940:three_prime_UTR 40.0%
CAATCATGACATTGAGGACA+AGG + tig0021765:98891-98910 None:intergenic 40.0%
GAATATCCCGTTGCTTCTTT+GGG - tig0021765:98936-98955 Msa1393940:CDS 40.0%
AACTTACCCAAAGAAGCAAC+GGG + tig0021765:98945-98964 None:intergenic 40.0%
!! TTGCCTTGTTTAGCTCAACA+CGG - tig0021765:99303-99322 Msa1393940:intron 40.0%
! TTGTTTAGCTCAACACGGTA+CGG - tig0021765:99308-99327 Msa1393940:intron 40.0%
! TTCACCACCATGTTGTCTTT+TGG - tig0021765:99567-99586 Msa1393940:intron 40.0%
ATGTCCAAAAGACAACATGG+TGG + tig0021765:99574-99593 None:intergenic 40.0%
GAGACATGATCCAACAATGA+AGG - tig0021765:99638-99657 Msa1393940:intron 40.0%
ACATGATCCAACAATGAAGG+AGG - tig0021765:99641-99660 Msa1393940:intron 40.0%
! GAAGTAGAAAAGTGCAGCAT+AGG - tig0021765:99795-99814 Msa1393940:intron 40.0%
TGGAAACTTGTGAAGAAGCA+AGG - tig0021765:99820-99839 Msa1393940:intron 40.0%
GGAAACTTGTGAAGAAGCAA+GGG - tig0021765:99821-99840 Msa1393940:intron 40.0%
AAGAACGAGCTCGTGATTAT+GGG - tig0021765:99895-99914 Msa1393940:CDS 40.0%
!!! GTTTTAAGGCATAGCCTACA+TGG + tig0021765:100044-100063 None:intergenic 40.0%
!! GTTTGGTTTGGTTTGCAATG+AGG - tig0021765:98739-98758 Msa1393940:three_prime_UTR 40.0%
!!! TGGTGTTTTGAAGTGGGTTT+TGG - tig0021765:98797-98816 Msa1393940:three_prime_UTR 40.0%
CAATCATGACATTGAGGACA+AGG + tig0021765:98891-98910 None:intergenic 40.0%
GAATATCCCGTTGCTTCTTT+GGG - tig0021765:98936-98955 Msa1393940:CDS 40.0%
AACTTACCCAAAGAAGCAAC+GGG + tig0021765:98945-98964 None:intergenic 40.0%
!! TTGCCTTGTTTAGCTCAACA+CGG - tig0021765:99303-99322 Msa1393940:intron 40.0%
! TTGTTTAGCTCAACACGGTA+CGG - tig0021765:99308-99327 Msa1393940:intron 40.0%
! TTCACCACCATGTTGTCTTT+TGG - tig0021765:99567-99586 Msa1393940:intron 40.0%
ATGTCCAAAAGACAACATGG+TGG + tig0021765:99574-99593 None:intergenic 40.0%
GAGACATGATCCAACAATGA+AGG - tig0021765:99638-99657 Msa1393940:intron 40.0%
ACATGATCCAACAATGAAGG+AGG - tig0021765:99641-99660 Msa1393940:intron 40.0%
! GAAGTAGAAAAGTGCAGCAT+AGG - tig0021765:99795-99814 Msa1393940:intron 40.0%
TGGAAACTTGTGAAGAAGCA+AGG - tig0021765:99820-99839 Msa1393940:intron 40.0%
GGAAACTTGTGAAGAAGCAA+GGG - tig0021765:99821-99840 Msa1393940:intron 40.0%
AAGAACGAGCTCGTGATTAT+GGG - tig0021765:99895-99914 Msa1393940:CDS 40.0%
!!! GTTTTAAGGCATAGCCTACA+TGG + tig0021765:100044-100063 None:intergenic 40.0%
GTGGTCCAATTGTGAACACA+AGG + tig0021765:98842-98861 None:intergenic 45.0%
ATATTCCAGTGAGGCAAGTG+AGG + tig0021765:98922-98941 None:intergenic 45.0%
GGAATATCCCGTTGCTTCTT+TGG - tig0021765:98935-98954 Msa1393940:CDS 45.0%
GTACCGTGTTGAGCTAAACA+AGG + tig0021765:99309-99328 None:intergenic 45.0%
! TGTTTAGCTCAACACGGTAC+GGG - tig0021765:99309-99328 Msa1393940:intron 45.0%
TCCACTGACAATGCTATCAG+AGG - tig0021765:99677-99696 Msa1393940:intron 45.0%
TCCTCTGATAGCATTGTCAG+TGG + tig0021765:99681-99700 None:intergenic 45.0%
GGTCTGTTCCAAAGTCTCAT+TGG + tig0021765:99727-99746 None:intergenic 45.0%
! AGAAAAGTGCAGCATAGGGA+TGG - tig0021765:99800-99819 Msa1393940:intron 45.0%
GAAGAACGAGCTCGTGATTA+TGG - tig0021765:99894-99913 Msa1393940:CDS 45.0%
GTGAATTCCACTTAACGGCA+GGG - tig0021765:100079-100098 Msa1393940:five_prime_UTR 45.0%
GTGGTCCAATTGTGAACACA+AGG + tig0021765:98842-98861 None:intergenic 45.0%
ATATTCCAGTGAGGCAAGTG+AGG + tig0021765:98922-98941 None:intergenic 45.0%
GGAATATCCCGTTGCTTCTT+TGG - tig0021765:98935-98954 Msa1393940:CDS 45.0%
GTACCGTGTTGAGCTAAACA+AGG + tig0021765:99309-99328 None:intergenic 45.0%
! TGTTTAGCTCAACACGGTAC+GGG - tig0021765:99309-99328 Msa1393940:intron 45.0%
TCCACTGACAATGCTATCAG+AGG - tig0021765:99677-99696 Msa1393940:intron 45.0%
TCCTCTGATAGCATTGTCAG+TGG + tig0021765:99681-99700 None:intergenic 45.0%
GGTCTGTTCCAAAGTCTCAT+TGG + tig0021765:99727-99746 None:intergenic 45.0%
! AGAAAAGTGCAGCATAGGGA+TGG - tig0021765:99800-99819 Msa1393940:intron 45.0%
GAAGAACGAGCTCGTGATTA+TGG - tig0021765:99894-99913 Msa1393940:CDS 45.0%
GTGAATTCCACTTAACGGCA+GGG - tig0021765:100079-100098 Msa1393940:five_prime_UTR 45.0%
TTCTTCAAACGCCAGCGAGA+TGG + tig0021765:98864-98883 None:intergenic 50.0%
AGCAACGGGATATTCCAGTG+AGG + tig0021765:98931-98950 None:intergenic 50.0%
! GTTTAGCTCAACACGGTACG+GGG - tig0021765:99310-99329 Msa1393940:intron 50.0%
! TGTTGAAGAGCGCAGGTTGA+CGG - tig0021765:99863-99882 Msa1393940:CDS 50.0%
AACGAGCTCGTGATTATGGG+TGG - tig0021765:99898-99917 Msa1393940:CDS 50.0%
AGCTCGTGATTATGGGTGGA+AGG - tig0021765:99902-99921 Msa1393940:CDS 50.0%
GTGATTATGGGTGGAAGGAC+AGG - tig0021765:99907-99926 Msa1393940:CDS 50.0%
GGTGAATTCCACTTAACGGC+AGG - tig0021765:100078-100097 Msa1393940:five_prime_UTR 50.0%
TTCTTCAAACGCCAGCGAGA+TGG + tig0021765:98864-98883 None:intergenic 50.0%
AGCAACGGGATATTCCAGTG+AGG + tig0021765:98931-98950 None:intergenic 50.0%
! GTTTAGCTCAACACGGTACG+GGG - tig0021765:99310-99329 Msa1393940:intron 50.0%
! TGTTGAAGAGCGCAGGTTGA+CGG - tig0021765:99863-99882 Msa1393940:CDS 50.0%
AACGAGCTCGTGATTATGGG+TGG - tig0021765:99898-99917 Msa1393940:CDS 50.0%
AGCTCGTGATTATGGGTGGA+AGG - tig0021765:99902-99921 Msa1393940:CDS 50.0%
GTGATTATGGGTGGAAGGAC+AGG - tig0021765:99907-99926 Msa1393940:CDS 50.0%
GGTGAATTCCACTTAACGGC+AGG - tig0021765:100078-100097 Msa1393940:five_prime_UTR 50.0%
CAATTGGACCACCATCTCGC+TGG - tig0021765:98850-98869 Msa1393940:exon 55.0%
TTCAAACGCCAGCGAGATGG+TGG + tig0021765:98861-98880 None:intergenic 55.0%
CTGTTCCTCACTTGCCTCAC+TGG - tig0021765:98914-98933 Msa1393940:CDS 55.0%
AGAAGCAAGGGAGAGAGCAG+AGG - tig0021765:99833-99852 Msa1393940:CDS 55.0%
!! CAGAGCTTGTTGAAGAGCGC+AGG - tig0021765:99856-99875 Msa1393940:CDS 55.0%
! AGCGCAGGTTGACGGCATTA+TGG - tig0021765:99871-99890 Msa1393940:CDS 55.0%
! GCGCAGGTTGACGGCATTAT+GGG - tig0021765:99872-99891 Msa1393940:CDS 55.0%
CAATTGGACCACCATCTCGC+TGG - tig0021765:98850-98869 Msa1393940:exon 55.0%
TTCAAACGCCAGCGAGATGG+TGG + tig0021765:98861-98880 None:intergenic 55.0%
CTGTTCCTCACTTGCCTCAC+TGG - tig0021765:98914-98933 Msa1393940:CDS 55.0%
AGAAGCAAGGGAGAGAGCAG+AGG - tig0021765:99833-99852 Msa1393940:CDS 55.0%
!! CAGAGCTTGTTGAAGAGCGC+AGG - tig0021765:99856-99875 Msa1393940:CDS 55.0%
! AGCGCAGGTTGACGGCATTA+TGG - tig0021765:99871-99890 Msa1393940:CDS 55.0%
! GCGCAGGTTGACGGCATTAT+GGG - tig0021765:99872-99891 Msa1393940:CDS 55.0%
GCTCAACACGGTACGGGGAT+TGG - tig0021765:99315-99334 Msa1393940:intron 60.0%
GCTCAACACGGTACGGGGAT+TGG - tig0021765:99315-99334 Msa1393940:intron 60.0%
Chromosome Type Strat End Strand Name
tig0021765 gene 98680 100107 98680 ID=Msa1393940;Name=Msa1393940
tig0021765 mRNA 98680 100107 98680 ID=Msa1393940-mRNA-1;Parent=Msa1393940;Name=Msa1393940-mRNA-1;_AED=0.00;_eAED=0.00;_QI=76|1|1|1|1|1|2|172|168
tig0021765 exon 98680 99156 98680 ID=Msa1393940-mRNA-1:exon:14110;Parent=Msa1393940-mRNA-1
tig0021765 exon 99830 100107 99830 ID=Msa1393940-mRNA-1:exon:14109;Parent=Msa1393940-mRNA-1
tig0021765 five_prime_UTR 100032 100107 100032 ID=Msa1393940-mRNA-1:five_prime_utr;Parent=Msa1393940-mRNA-1
tig0021765 CDS 99830 100031 99830 ID=Msa1393940-mRNA-1:cds;Parent=Msa1393940-mRNA-1
tig0021765 CDS 98852 99156 98852 ID=Msa1393940-mRNA-1:cds;Parent=Msa1393940-mRNA-1
tig0021765 three_prime_UTR 98680 98851 98680 ID=Msa1393940-mRNA-1:three_prime_utr;Parent=Msa1393940-mRNA-1
Gene Sequence

>Msa1393940

ATGAGGGTTCATCATCAAAATGAGCAAAGACAATGTTCTTATGGTGTTTTGAAGTGGGTTTTGGTTTGGATGAGTGTGTGTTACCTTGTGTTCACAATTGGACCACCATCTCGCTGGCGTTTGAAGAAATCTCCTTGTCCTCAATGTCATGATTGTTACTGTTCCTCACTTGCCTCACTGGAATATCCCGTTGCTTCTTTGGATTGTGGGAGACATGATCCAACAATGAAGGAGGAAATGAACAAGGATCCACTGACAATGCTATCAGAGGAGTTAAAGTTGCAGAAAATTGTAGCCAATGAGACTTTGGAACAGACCAAAAGATTAGTAATAAATGCAAGGAATACTTTCTCTCATTATAAAAAAGAAGTAGAAAAGTGCAGCATAGGGATGGAAACTTGTGAAGAAGCAAGGGAGAGAGCAGAGGCAGAGCTTGTTGAAGAGCGCAGGTTGACGGCATTATGGGAAGAACGAGCTCGTGATTATGGGTGGAAGGACAGGAGATGA
Protein sequence

>Msa1393940

MRVHHQNEQRQCSYGVLKWVLVWMSVCYLVFTIGPPSRWRLKKSPCPQCHDCYCSSLASLEYPVASLDCGRHDPTMKEEMNKDPLTMLSEELKLQKIVANETLEQTKRLVINARNTFSHYKKEVEKCSIGMETCEEARERAEAELVEERRLTALWEERARDYGWKDRR*