AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0267220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267220 MtrunA17_Chr7g0273171 91.667 168 10 2 1 168 1 164 3.95e-105 298
Msa0267220 MtrunA17_Chr2g0288981 39.053 169 90 4 7 165 2 167 8.12e-32 112
Msa0267220 MtrunA17_Chr4g0025231 35.429 175 88 3 13 166 7 177 3.03e-30 108
Msa0267220 MtrunA17_Chr2g0304821 36.196 163 91 2 17 166 14 176 7.43e-26 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267220 AT1G05070.1 38.922 167 83 5 15 166 6 168 1.17e-32 115
Msa0267220 AT2G32580.1 37.423 163 90 3 15 166 6 167 3.24e-31 111
Msa0267220 AT4G04360.1 42.236 161 79 4 18 166 10 168 4.84e-29 105
Msa0267220 AT2G24290.1 40.373 161 81 3 21 166 12 172 6.89e-29 105
Msa0267220 AT4G30996.1 37.736 159 85 2 21 165 12 170 1.10e-27 102
Msa0267220 AT2G32580.2 43.000 100 57 0 67 166 6 105 3.15e-24 91.7

Find 54 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAACTAAATTAGTATTT+TGG 0.141693 2_2:+68715143 Msa0267220:three_prime_UTR
GGAATATCCCGTTGCTTCTT+TGG 0.212587 2_2:+68714021 Msa0267220:CDS
TGCATTTATTACTAATCTTT+TGG 0.212992 2_2:-68714831 None:intergenic
GAAGAACGAGCTCGTGATTA+TGG 0.234749 2_2:+68714980 Msa0267220:CDS
TTCATTTCCTCCTTCATTGT+TGG 0.237361 2_2:-68714734 None:intergenic
AGCGCAGGTTGACGGCATTA+TGG 0.273292 2_2:+68714957 Msa0267220:CDS
GAGCAAAGACAATGTTCTTA+TGG 0.346328 2_2:+68713863 Msa0267220:CDS
CTGTTCCTCACTTGCCTCAC+TGG 0.367272 2_2:+68714000 Msa0267220:CDS
TGTTACCTTGTGTTCACAAT+TGG 0.371180 2_2:+68713920 Msa0267220:CDS
GAATATCCCGTTGCTTCTTT+GGG 0.376906 2_2:+68714022 Msa0267220:CDS
GCGCAGGTTGACGGCATTAT+GGG 0.391190 2_2:+68714958 Msa0267220:CDS
TTACTATATGTACAGATTGT+GGG 0.418392 2_2:+68714702 Msa0267220:intron
GTGACTGGTTTGGTTTGGTT+TGG 0.418626 2_2:+68713813 Msa0267220:five_prime_UTR
AGTAGTGATAGTTTATTAGT+TGG 0.424734 2_2:-68715045 None:intergenic
GAAGTAGAAAAGTGCAGCAT+AGG 0.426789 2_2:+68714881 Msa0267220:CDS
CAGAGCTTGTTGAAGAGCGC+AGG 0.429709 2_2:+68714942 Msa0267220:CDS
TTTGGTTTGGTTTGCAATGA+GGG 0.434025 2_2:+68713826 Msa0267220:exon
ATTGAGTGACTGGTTTGGTT+TGG 0.434502 2_2:+68713808 Msa0267220:five_prime_UTR
AAGAACGAGCTCGTGATTAT+GGG 0.441049 2_2:+68714981 Msa0267220:CDS
GGTCTGTTCCAAAGTCTCAT+TGG 0.443502 2_2:-68714810 None:intergenic
TTTAAGACCCTGCCGTTAAG+TGG 0.447186 2_2:-68715172 None:intergenic
AATTGTAGCCAATGAGACTT+TGG 0.447499 2_2:+68714802 Msa0267220:CDS
ACAGAGTTCAATTGAGTGAC+TGG 0.450522 2_2:+68713798 Msa0267220:five_prime_UTR
GTGATTATGGGTGGAAGGAC+AGG 0.457853 2_2:+68714993 Msa0267220:CDS
GTTCAATTGAGTGACTGGTT+TGG 0.492672 2_2:+68713803 Msa0267220:five_prime_UTR
AACTTACCCAAAGAAGCAAC+GGG 0.499220 2_2:-68714028 None:intergenic
TGTTGAAGAGCGCAGGTTGA+CGG 0.500440 2_2:+68714949 Msa0267220:CDS
GGTGAATTCCACTTAACGGC+AGG 0.511432 2_2:+68715164 Msa0267220:three_prime_UTR
AAGTAGAAAAGTGCAGCATA+GGG 0.518644 2_2:+68714882 Msa0267220:CDS
GTTTGGTTTGGTTTGCAATG+AGG 0.525335 2_2:+68713825 Msa0267220:exon
TTCTTCAAACGCCAGCGAGA+TGG 0.541960 2_2:-68713947 None:intergenic
AGAAAAGTGCAGCATAGGGA+TGG 0.556983 2_2:+68714886 Msa0267220:CDS
CAATCATGACATTGAGGACA+AGG 0.561176 2_2:-68713974 None:intergenic
CAATTGGACCACCATCTCGC+TGG 0.563845 2_2:+68713936 Msa0267220:CDS
TGGAAACTTGTGAAGAAGCA+AGG 0.573605 2_2:+68714906 Msa0267220:CDS
TTCAAACGCCAGCGAGATGG+TGG 0.580007 2_2:-68713944 None:intergenic
TAATTGTGATTATTTAACCA+TGG 0.581787 2_2:+68715110 Msa0267220:three_prime_UTR
AGAAGCAAGGGAGAGAGCAG+AGG 0.585238 2_2:+68714919 Msa0267220:CDS
GAGACATGATCCAACAATGA+AGG 0.590741 2_2:+68714724 Msa0267220:CDS
AGCTCGTGATTATGGGTGGA+AGG 0.596678 2_2:+68714988 Msa0267220:CDS
AAAGATTAGTAATAAATGCA+AGG 0.596837 2_2:+68714834 Msa0267220:CDS
TCCTCTGATAGCATTGTCAG+TGG 0.601328 2_2:-68714764 None:intergenic
AAACTTACCCAAAGAAGCAA+CGG 0.604283 2_2:-68714029 None:intergenic
AACGAGCTCGTGATTATGGG+TGG 0.612321 2_2:+68714984 Msa0267220:CDS
GTGAATTCCACTTAACGGCA+GGG 0.613965 2_2:+68715165 Msa0267220:three_prime_UTR
GGAAACTTGTGAAGAAGCAA+GGG 0.615984 2_2:+68714907 Msa0267220:CDS
TTGTGATTATTTAACCATGG+AGG 0.625180 2_2:+68715113 Msa0267220:three_prime_UTR
CAGTAACAATCATGACATTG+AGG 0.634830 2_2:-68713980 None:intergenic
TCCACTGACAATGCTATCAG+AGG 0.642154 2_2:+68714763 Msa0267220:CDS
ATATTCCAGTGAGGCAAGTG+AGG 0.642772 2_2:-68714005 None:intergenic
ACATGATCCAACAATGAAGG+AGG 0.647322 2_2:+68714727 Msa0267220:CDS
GTGGTCCAATTGTGAACACA+AGG 0.658093 2_2:-68713925 None:intergenic
AATGAAGGAGGAAATGAACA+AGG 0.667487 2_2:+68714739 Msa0267220:CDS
AGCAACGGGATATTCCAGTG+AGG 0.784097 2_2:-68714014 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAAATTTTACTTAAAG+TGG - chr2_2:68714560-68714579 None:intergenic 10.0%
!!! AAAATACTAATTTAGTTTTA+AGG - chr2_2:68715144-68715163 None:intergenic 10.0%
!!! TTAAAACTAAATTAGTATTT+TGG + chr2_2:68715143-68715162 Msa0267220:three_prime_UTR 10.0%
!!! ATTATAAATTTTACTTAAAG+TGG - chr2_2:68714560-68714579 None:intergenic 10.0%
!!! AAAATACTAATTTAGTTTTA+AGG - chr2_2:68715144-68715163 None:intergenic 10.0%
!!! TTAAAACTAAATTAGTATTT+TGG + chr2_2:68715143-68715162 Msa0267220:three_prime_UTR 10.0%
!! TAGTAATTAGAAAAATTCAC+TGG + chr2_2:68714164-68714183 Msa0267220:intron 20.0%
!! CAATTCAATTCATATGTATT+AGG + chr2_2:68714251-68714270 Msa0267220:intron 20.0%
!! TAGGTTTATATATTAATTCG+TGG + chr2_2:68714270-68714289 Msa0267220:intron 20.0%
!! AGAAATGCAAAACATTTAAA+TGG - chr2_2:68714471-68714490 None:intergenic 20.0%
!!! TGCATTTATTACTAATCTTT+TGG - chr2_2:68714834-68714853 None:intergenic 20.0%
!! AAAGATTAGTAATAAATGCA+AGG + chr2_2:68714834-68714853 Msa0267220:CDS 20.0%
!! TAATTGTGATTATTTAACCA+TGG + chr2_2:68715110-68715129 Msa0267220:three_prime_UTR 20.0%
!! TAGTAATTAGAAAAATTCAC+TGG + chr2_2:68714164-68714183 Msa0267220:intron 20.0%
!! CAATTCAATTCATATGTATT+AGG + chr2_2:68714251-68714270 Msa0267220:intron 20.0%
!! TAGGTTTATATATTAATTCG+TGG + chr2_2:68714270-68714289 Msa0267220:intron 20.0%
!! AGAAATGCAAAACATTTAAA+TGG - chr2_2:68714471-68714490 None:intergenic 20.0%
!!! TGCATTTATTACTAATCTTT+TGG - chr2_2:68714834-68714853 None:intergenic 20.0%
!! AAAGATTAGTAATAAATGCA+AGG + chr2_2:68714834-68714853 Msa0267220:CDS 20.0%
!! TAATTGTGATTATTTAACCA+TGG + chr2_2:68715110-68715129 Msa0267220:three_prime_UTR 20.0%
!!! GTTTTGTTTTGTTTTGTTTG+AGG - chr2_2:68713770-68713789 None:intergenic 25.0%
! TTTGTAGAGTAAGTGAAAAA+GGG - chr2_2:68714055-68714074 None:intergenic 25.0%
! ATTTGTAGAGTAAGTGAAAA+AGG - chr2_2:68714056-68714075 None:intergenic 25.0%
!!! CATCATTTTTCAAACACAAA+GGG - chr2_2:68714141-68714160 None:intergenic 25.0%
! ATTGAATAACCAAAACATTC+TGG - chr2_2:68714235-68714254 None:intergenic 25.0%
! TTTACTATATGTACAGATTG+TGG + chr2_2:68714701-68714720 Msa0267220:intron 25.0%
! TTACTATATGTACAGATTGT+GGG + chr2_2:68714702-68714721 Msa0267220:intron 25.0%
! AGTAGTGATAGTTTATTAGT+TGG - chr2_2:68715048-68715067 None:intergenic 25.0%
!!! GTTTTGTTTTGTTTTGTTTG+AGG - chr2_2:68713770-68713789 None:intergenic 25.0%
! TTTGTAGAGTAAGTGAAAAA+GGG - chr2_2:68714055-68714074 None:intergenic 25.0%
! ATTTGTAGAGTAAGTGAAAA+AGG - chr2_2:68714056-68714075 None:intergenic 25.0%
!!! CATCATTTTTCAAACACAAA+GGG - chr2_2:68714141-68714160 None:intergenic 25.0%
! ATTGAATAACCAAAACATTC+TGG - chr2_2:68714235-68714254 None:intergenic 25.0%
! TTTACTATATGTACAGATTG+TGG + chr2_2:68714701-68714720 Msa0267220:intron 25.0%
! TTACTATATGTACAGATTGT+GGG + chr2_2:68714702-68714721 Msa0267220:intron 25.0%
! AGTAGTGATAGTTTATTAGT+TGG - chr2_2:68715048-68715067 None:intergenic 25.0%
!!! TTCTTATGGTGTTTTGAAGT+GGG + chr2_2:68713877-68713896 Msa0267220:CDS 30.0%
ACTCTACAAATTAACAGACT+TGG + chr2_2:68714065-68714084 Msa0267220:intron 30.0%
ACAAGTTTGATTAGATGTAC+AGG + chr2_2:68714104-68714123 Msa0267220:intron 30.0%
!! GCATCATTTTTCAAACACAA+AGG - chr2_2:68714142-68714161 None:intergenic 30.0%
CTTTGTTCTGATTCTCAAAA+AGG - chr2_2:68714193-68714212 None:intergenic 30.0%
! ATGTATGTTTTGTATCACAG+TGG - chr2_2:68714361-68714380 None:intergenic 30.0%
TGATACAAAACATACATCGA+AGG + chr2_2:68714364-68714383 Msa0267220:intron 30.0%
CTAAAAATGAGAAGAGAATG+AGG - chr2_2:68714620-68714639 None:intergenic 30.0%
ATAATGTCCAAAAGACAACA+TGG - chr2_2:68714663-68714682 None:intergenic 30.0%
TTGTGATTATTTAACCATGG+AGG + chr2_2:68715113-68715132 Msa0267220:three_prime_UTR 30.0%
! TTTTGGTGAATTCCACTTAA+CGG + chr2_2:68715160-68715179 Msa0267220:three_prime_UTR 30.0%
!!! TTCTTATGGTGTTTTGAAGT+GGG + chr2_2:68713877-68713896 Msa0267220:CDS 30.0%
ACTCTACAAATTAACAGACT+TGG + chr2_2:68714065-68714084 Msa0267220:intron 30.0%
ACAAGTTTGATTAGATGTAC+AGG + chr2_2:68714104-68714123 Msa0267220:intron 30.0%
!! GCATCATTTTTCAAACACAA+AGG - chr2_2:68714142-68714161 None:intergenic 30.0%
CTTTGTTCTGATTCTCAAAA+AGG - chr2_2:68714193-68714212 None:intergenic 30.0%
! ATGTATGTTTTGTATCACAG+TGG - chr2_2:68714361-68714380 None:intergenic 30.0%
TGATACAAAACATACATCGA+AGG + chr2_2:68714364-68714383 Msa0267220:intron 30.0%
CTAAAAATGAGAAGAGAATG+AGG - chr2_2:68714620-68714639 None:intergenic 30.0%
ATAATGTCCAAAAGACAACA+TGG - chr2_2:68714663-68714682 None:intergenic 30.0%
TTGTGATTATTTAACCATGG+AGG + chr2_2:68715113-68715132 Msa0267220:three_prime_UTR 30.0%
! TTTTGGTGAATTCCACTTAA+CGG + chr2_2:68715160-68715179 Msa0267220:three_prime_UTR 30.0%
!! TTTGGTTTGGTTTGCAATGA+GGG + chr2_2:68713826-68713845 Msa0267220:exon 35.0%
GAGCAAAGACAATGTTCTTA+TGG + chr2_2:68713863-68713882 Msa0267220:CDS 35.0%
!!! GTTCTTATGGTGTTTTGAAG+TGG + chr2_2:68713876-68713895 Msa0267220:CDS 35.0%
!!! TTTTGAAGTGGGTTTTGGTT+TGG + chr2_2:68713888-68713907 Msa0267220:CDS 35.0%
TGTTACCTTGTGTTCACAAT+TGG + chr2_2:68713920-68713939 Msa0267220:CDS 35.0%
CAGTAACAATCATGACATTG+AGG - chr2_2:68713983-68714002 None:intergenic 35.0%
AAACTTACCCAAAGAAGCAA+CGG - chr2_2:68714032-68714051 None:intergenic 35.0%
! TCTATCTAGCCAGAATGTTT+TGG + chr2_2:68714223-68714242 Msa0267220:intron 35.0%
!! CATAGCTAGCTTTGTTTATG+TGG + chr2_2:68714496-68714515 Msa0267220:intron 35.0%
TTCATTTCCTCCTTCATTGT+TGG - chr2_2:68714737-68714756 None:intergenic 35.0%
AATGAAGGAGGAAATGAACA+AGG + chr2_2:68714739-68714758 Msa0267220:CDS 35.0%
AATTGTAGCCAATGAGACTT+TGG + chr2_2:68714802-68714821 Msa0267220:CDS 35.0%
! AAGTAGAAAAGTGCAGCATA+GGG + chr2_2:68714882-68714901 Msa0267220:CDS 35.0%
!! TTTGGTTTGGTTTGCAATGA+GGG + chr2_2:68713826-68713845 Msa0267220:exon 35.0%
GAGCAAAGACAATGTTCTTA+TGG + chr2_2:68713863-68713882 Msa0267220:CDS 35.0%
!!! GTTCTTATGGTGTTTTGAAG+TGG + chr2_2:68713876-68713895 Msa0267220:CDS 35.0%
!!! TTTTGAAGTGGGTTTTGGTT+TGG + chr2_2:68713888-68713907 Msa0267220:CDS 35.0%
TGTTACCTTGTGTTCACAAT+TGG + chr2_2:68713920-68713939 Msa0267220:CDS 35.0%
CAGTAACAATCATGACATTG+AGG - chr2_2:68713983-68714002 None:intergenic 35.0%
AAACTTACCCAAAGAAGCAA+CGG - chr2_2:68714032-68714051 None:intergenic 35.0%
! TCTATCTAGCCAGAATGTTT+TGG + chr2_2:68714223-68714242 Msa0267220:intron 35.0%
!! CATAGCTAGCTTTGTTTATG+TGG + chr2_2:68714496-68714515 Msa0267220:intron 35.0%
TTCATTTCCTCCTTCATTGT+TGG - chr2_2:68714737-68714756 None:intergenic 35.0%
AATGAAGGAGGAAATGAACA+AGG + chr2_2:68714739-68714758 Msa0267220:CDS 35.0%
AATTGTAGCCAATGAGACTT+TGG + chr2_2:68714802-68714821 Msa0267220:CDS 35.0%
! AAGTAGAAAAGTGCAGCATA+GGG + chr2_2:68714882-68714901 Msa0267220:CDS 35.0%
ACAGAGTTCAATTGAGTGAC+TGG + chr2_2:68713798-68713817 Msa0267220:five_prime_UTR 40.0%
! GTTCAATTGAGTGACTGGTT+TGG + chr2_2:68713803-68713822 Msa0267220:five_prime_UTR 40.0%
!! ATTGAGTGACTGGTTTGGTT+TGG + chr2_2:68713808-68713827 Msa0267220:five_prime_UTR 40.0%
!! GTTTGGTTTGGTTTGCAATG+AGG + chr2_2:68713825-68713844 Msa0267220:exon 40.0%
!!! TGGTGTTTTGAAGTGGGTTT+TGG + chr2_2:68713883-68713902 Msa0267220:CDS 40.0%
CAATCATGACATTGAGGACA+AGG - chr2_2:68713977-68713996 None:intergenic 40.0%
GAATATCCCGTTGCTTCTTT+GGG + chr2_2:68714022-68714041 Msa0267220:CDS 40.0%
AACTTACCCAAAGAAGCAAC+GGG - chr2_2:68714031-68714050 None:intergenic 40.0%
!! TTGCCTTGTTTAGCTCAACA+CGG + chr2_2:68714389-68714408 Msa0267220:intron 40.0%
! TTGTTTAGCTCAACACGGTA+CGG + chr2_2:68714394-68714413 Msa0267220:intron 40.0%
! TTCACCACCATGTTGTCTTT+TGG + chr2_2:68714653-68714672 Msa0267220:intron 40.0%
ATGTCCAAAAGACAACATGG+TGG - chr2_2:68714660-68714679 None:intergenic 40.0%
GAGACATGATCCAACAATGA+AGG + chr2_2:68714724-68714743 Msa0267220:CDS 40.0%
ACATGATCCAACAATGAAGG+AGG + chr2_2:68714727-68714746 Msa0267220:CDS 40.0%
! GAAGTAGAAAAGTGCAGCAT+AGG + chr2_2:68714881-68714900 Msa0267220:CDS 40.0%
TGGAAACTTGTGAAGAAGCA+AGG + chr2_2:68714906-68714925 Msa0267220:CDS 40.0%
GGAAACTTGTGAAGAAGCAA+GGG + chr2_2:68714907-68714926 Msa0267220:CDS 40.0%
AAGAACGAGCTCGTGATTAT+GGG + chr2_2:68714981-68715000 Msa0267220:CDS 40.0%
ACAGAGTTCAATTGAGTGAC+TGG + chr2_2:68713798-68713817 Msa0267220:five_prime_UTR 40.0%
! GTTCAATTGAGTGACTGGTT+TGG + chr2_2:68713803-68713822 Msa0267220:five_prime_UTR 40.0%
!! ATTGAGTGACTGGTTTGGTT+TGG + chr2_2:68713808-68713827 Msa0267220:five_prime_UTR 40.0%
!! GTTTGGTTTGGTTTGCAATG+AGG + chr2_2:68713825-68713844 Msa0267220:exon 40.0%
!!! TGGTGTTTTGAAGTGGGTTT+TGG + chr2_2:68713883-68713902 Msa0267220:CDS 40.0%
CAATCATGACATTGAGGACA+AGG - chr2_2:68713977-68713996 None:intergenic 40.0%
GAATATCCCGTTGCTTCTTT+GGG + chr2_2:68714022-68714041 Msa0267220:CDS 40.0%
AACTTACCCAAAGAAGCAAC+GGG - chr2_2:68714031-68714050 None:intergenic 40.0%
!! TTGCCTTGTTTAGCTCAACA+CGG + chr2_2:68714389-68714408 Msa0267220:intron 40.0%
! TTGTTTAGCTCAACACGGTA+CGG + chr2_2:68714394-68714413 Msa0267220:intron 40.0%
! TTCACCACCATGTTGTCTTT+TGG + chr2_2:68714653-68714672 Msa0267220:intron 40.0%
ATGTCCAAAAGACAACATGG+TGG - chr2_2:68714660-68714679 None:intergenic 40.0%
GAGACATGATCCAACAATGA+AGG + chr2_2:68714724-68714743 Msa0267220:CDS 40.0%
ACATGATCCAACAATGAAGG+AGG + chr2_2:68714727-68714746 Msa0267220:CDS 40.0%
! GAAGTAGAAAAGTGCAGCAT+AGG + chr2_2:68714881-68714900 Msa0267220:CDS 40.0%
TGGAAACTTGTGAAGAAGCA+AGG + chr2_2:68714906-68714925 Msa0267220:CDS 40.0%
GGAAACTTGTGAAGAAGCAA+GGG + chr2_2:68714907-68714926 Msa0267220:CDS 40.0%
AAGAACGAGCTCGTGATTAT+GGG + chr2_2:68714981-68715000 Msa0267220:CDS 40.0%
!! GTGACTGGTTTGGTTTGGTT+TGG + chr2_2:68713813-68713832 Msa0267220:five_prime_UTR 45.0%
GTGGTCCAATTGTGAACACA+AGG - chr2_2:68713928-68713947 None:intergenic 45.0%
ATATTCCAGTGAGGCAAGTG+AGG - chr2_2:68714008-68714027 None:intergenic 45.0%
GGAATATCCCGTTGCTTCTT+TGG + chr2_2:68714021-68714040 Msa0267220:CDS 45.0%
GTACCGTGTTGAGCTAAACA+AGG - chr2_2:68714395-68714414 None:intergenic 45.0%
! TGTTTAGCTCAACACGGTAC+GGG + chr2_2:68714395-68714414 Msa0267220:intron 45.0%
TCCACTGACAATGCTATCAG+AGG + chr2_2:68714763-68714782 Msa0267220:CDS 45.0%
TCCTCTGATAGCATTGTCAG+TGG - chr2_2:68714767-68714786 None:intergenic 45.0%
GGTCTGTTCCAAAGTCTCAT+TGG - chr2_2:68714813-68714832 None:intergenic 45.0%
! AGAAAAGTGCAGCATAGGGA+TGG + chr2_2:68714886-68714905 Msa0267220:CDS 45.0%
GAAGAACGAGCTCGTGATTA+TGG + chr2_2:68714980-68714999 Msa0267220:CDS 45.0%
!!! GTTTTAAGGCATAGCCTCCA+TGG - chr2_2:68715130-68715149 None:intergenic 45.0%
GTGAATTCCACTTAACGGCA+GGG + chr2_2:68715165-68715184 Msa0267220:three_prime_UTR 45.0%
!! GTGACTGGTTTGGTTTGGTT+TGG + chr2_2:68713813-68713832 Msa0267220:five_prime_UTR 45.0%
GTGGTCCAATTGTGAACACA+AGG - chr2_2:68713928-68713947 None:intergenic 45.0%
ATATTCCAGTGAGGCAAGTG+AGG - chr2_2:68714008-68714027 None:intergenic 45.0%
GGAATATCCCGTTGCTTCTT+TGG + chr2_2:68714021-68714040 Msa0267220:CDS 45.0%
GTACCGTGTTGAGCTAAACA+AGG - chr2_2:68714395-68714414 None:intergenic 45.0%
! TGTTTAGCTCAACACGGTAC+GGG + chr2_2:68714395-68714414 Msa0267220:intron 45.0%
TCCACTGACAATGCTATCAG+AGG + chr2_2:68714763-68714782 Msa0267220:CDS 45.0%
TCCTCTGATAGCATTGTCAG+TGG - chr2_2:68714767-68714786 None:intergenic 45.0%
GGTCTGTTCCAAAGTCTCAT+TGG - chr2_2:68714813-68714832 None:intergenic 45.0%
! AGAAAAGTGCAGCATAGGGA+TGG + chr2_2:68714886-68714905 Msa0267220:CDS 45.0%
GAAGAACGAGCTCGTGATTA+TGG + chr2_2:68714980-68714999 Msa0267220:CDS 45.0%
!!! GTTTTAAGGCATAGCCTCCA+TGG - chr2_2:68715130-68715149 None:intergenic 45.0%
GTGAATTCCACTTAACGGCA+GGG + chr2_2:68715165-68715184 Msa0267220:three_prime_UTR 45.0%
TTCTTCAAACGCCAGCGAGA+TGG - chr2_2:68713950-68713969 None:intergenic 50.0%
AGCAACGGGATATTCCAGTG+AGG - chr2_2:68714017-68714036 None:intergenic 50.0%
! GTTTAGCTCAACACGGTACG+GGG + chr2_2:68714396-68714415 Msa0267220:intron 50.0%
! TGTTGAAGAGCGCAGGTTGA+CGG + chr2_2:68714949-68714968 Msa0267220:CDS 50.0%
AACGAGCTCGTGATTATGGG+TGG + chr2_2:68714984-68715003 Msa0267220:CDS 50.0%
AGCTCGTGATTATGGGTGGA+AGG + chr2_2:68714988-68715007 Msa0267220:CDS 50.0%
GTGATTATGGGTGGAAGGAC+AGG + chr2_2:68714993-68715012 Msa0267220:CDS 50.0%
GGTGAATTCCACTTAACGGC+AGG + chr2_2:68715164-68715183 Msa0267220:three_prime_UTR 50.0%
TTCTTCAAACGCCAGCGAGA+TGG - chr2_2:68713950-68713969 None:intergenic 50.0%
AGCAACGGGATATTCCAGTG+AGG - chr2_2:68714017-68714036 None:intergenic 50.0%
! GTTTAGCTCAACACGGTACG+GGG + chr2_2:68714396-68714415 Msa0267220:intron 50.0%
! TGTTGAAGAGCGCAGGTTGA+CGG + chr2_2:68714949-68714968 Msa0267220:CDS 50.0%
AACGAGCTCGTGATTATGGG+TGG + chr2_2:68714984-68715003 Msa0267220:CDS 50.0%
AGCTCGTGATTATGGGTGGA+AGG + chr2_2:68714988-68715007 Msa0267220:CDS 50.0%
GTGATTATGGGTGGAAGGAC+AGG + chr2_2:68714993-68715012 Msa0267220:CDS 50.0%
GGTGAATTCCACTTAACGGC+AGG + chr2_2:68715164-68715183 Msa0267220:three_prime_UTR 50.0%
CAATTGGACCACCATCTCGC+TGG + chr2_2:68713936-68713955 Msa0267220:CDS 55.0%
TTCAAACGCCAGCGAGATGG+TGG - chr2_2:68713947-68713966 None:intergenic 55.0%
CTGTTCCTCACTTGCCTCAC+TGG + chr2_2:68714000-68714019 Msa0267220:CDS 55.0%
AGAAGCAAGGGAGAGAGCAG+AGG + chr2_2:68714919-68714938 Msa0267220:CDS 55.0%
!! CAGAGCTTGTTGAAGAGCGC+AGG + chr2_2:68714942-68714961 Msa0267220:CDS 55.0%
! AGCGCAGGTTGACGGCATTA+TGG + chr2_2:68714957-68714976 Msa0267220:CDS 55.0%
! GCGCAGGTTGACGGCATTAT+GGG + chr2_2:68714958-68714977 Msa0267220:CDS 55.0%
CAATTGGACCACCATCTCGC+TGG + chr2_2:68713936-68713955 Msa0267220:CDS 55.0%
TTCAAACGCCAGCGAGATGG+TGG - chr2_2:68713947-68713966 None:intergenic 55.0%
CTGTTCCTCACTTGCCTCAC+TGG + chr2_2:68714000-68714019 Msa0267220:CDS 55.0%
AGAAGCAAGGGAGAGAGCAG+AGG + chr2_2:68714919-68714938 Msa0267220:CDS 55.0%
!! CAGAGCTTGTTGAAGAGCGC+AGG + chr2_2:68714942-68714961 Msa0267220:CDS 55.0%
! AGCGCAGGTTGACGGCATTA+TGG + chr2_2:68714957-68714976 Msa0267220:CDS 55.0%
! GCGCAGGTTGACGGCATTAT+GGG + chr2_2:68714958-68714977 Msa0267220:CDS 55.0%
GCTCAACACGGTACGGGGAT+TGG + chr2_2:68714401-68714420 Msa0267220:intron 60.0%
GCTCAACACGGTACGGGGAT+TGG + chr2_2:68714401-68714420 Msa0267220:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 68713762 68715193 68713762 ID=Msa0267220;Name=Msa0267220
chr2_2 mRNA 68713762 68715193 68713762 ID=Msa0267220-mRNA-1;Parent=Msa0267220;Name=Msa0267220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=80|1|1|1|1|1|2|172|168
chr2_2 exon 68713762 68714043 68713762 ID=Msa0267220-mRNA-1:exon:21794;Parent=Msa0267220-mRNA-1
chr2_2 exon 68714717 68715193 68714717 ID=Msa0267220-mRNA-1:exon:21795;Parent=Msa0267220-mRNA-1
chr2_2 five_prime_UTR 68713762 68713841 68713762 ID=Msa0267220-mRNA-1:five_prime_utr;Parent=Msa0267220-mRNA-1
chr2_2 CDS 68713842 68714043 68713842 ID=Msa0267220-mRNA-1:cds;Parent=Msa0267220-mRNA-1
chr2_2 CDS 68714717 68715021 68714717 ID=Msa0267220-mRNA-1:cds;Parent=Msa0267220-mRNA-1
chr2_2 three_prime_UTR 68715022 68715193 68715022 ID=Msa0267220-mRNA-1:three_prime_utr;Parent=Msa0267220-mRNA-1
Gene Sequence

>Msa0267220

ATGAGGGTTCATCATCAAAATGAGCAAAGACAATGTTCTTATGGTGTTTTGAAGTGGGTTTTGGTTTGGATGAGTGTGTGTTACCTTGTGTTCACAATTGGACCACCATCTCGCTGGCGTTTGAAGAAATCTCCTTGTCCTCAATGTCATGATTGTTACTGTTCCTCACTTGCCTCACTGGAATATCCCGTTGCTTCTTTGGATTGTGGGAGACATGATCCAACAATGAAGGAGGAAATGAACAAGGATCCACTGACAATGCTATCAGAGGAGTTAAAGTTGCAGAAAATTGTAGCCAATGAGACTTTGGAACAGACCAAAAGATTAGTAATAAATGCAAGGAATACTTTCTCTCATTATAAAAAAGAAGTAGAAAAGTGCAGCATAGGGATGGAAACTTGTGAAGAAGCAAGGGAGAGAGCAGAGGCAGAGCTTGTTGAAGAGCGCAGGTTGACGGCATTATGGGAAGAACGAGCTCGTGATTATGGGTGGAAGGACAGGAGATGA
Protein sequence

>Msa0267220

MRVHHQNEQRQCSYGVLKWVLVWMSVCYLVFTIGPPSRWRLKKSPCPQCHDCYCSSLASLEYPVASLDCGRHDPTMKEEMNKDPLTMLSEELKLQKIVANETLEQTKRLVINARNTFSHYKKEVEKCSIGMETCEEARERAEAELVEERRLTALWEERARDYGWKDRR*