AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1165020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1165020 MtrunA17_Chr7g0273171 91.667 168 10 2 1 168 1 164 3.95e-105 298
Msa1165020 MtrunA17_Chr2g0288981 39.053 169 90 4 7 165 2 167 8.12e-32 112
Msa1165020 MtrunA17_Chr4g0025231 35.429 175 88 3 13 166 7 177 3.03e-30 108
Msa1165020 MtrunA17_Chr2g0304821 36.196 163 91 2 17 166 14 176 7.43e-26 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1165020 AT1G05070.1 38.922 167 83 5 15 166 6 168 1.17e-32 115
Msa1165020 AT2G32580.1 37.423 163 90 3 15 166 6 167 3.24e-31 111
Msa1165020 AT4G04360.1 42.236 161 79 4 18 166 10 168 4.84e-29 105
Msa1165020 AT2G24290.1 40.373 161 81 3 21 166 12 172 6.89e-29 105
Msa1165020 AT4G30996.1 37.736 159 85 2 21 165 12 170 1.10e-27 102
Msa1165020 AT2G32580.2 43.000 100 57 0 67 166 6 105 3.15e-24 91.7

Find 52 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAACTAAATTAGTATTT+TGG 0.141693 7_4:-56862363 Msa1165020:three_prime_UTR
GGAATATCCCGTTGCTTCTT+TGG 0.212587 7_4:-56863485 Msa1165020:CDS
TGCATTTATTACTAATCTTT+TGG 0.212992 7_4:+56862675 None:intergenic
GAAGAACGAGCTCGTGATTA+TGG 0.234749 7_4:-56862526 Msa1165020:CDS
TTCATTTCCTCCTTCATTGT+TGG 0.237361 7_4:+56862772 None:intergenic
AGCGCAGGTTGACGGCATTA+TGG 0.273292 7_4:-56862549 Msa1165020:CDS
GTTCAATTGAGTGACAAATT+TGG 0.277581 7_4:-56863703 Msa1165020:five_prime_UTR
GAGCAAAGACAATGTTCTTA+TGG 0.346328 7_4:-56863643 Msa1165020:CDS
CTGTTCCTCACTTGCCTCAC+TGG 0.367272 7_4:-56863506 Msa1165020:CDS
TGTTACCTTGTGTTCACAAT+TGG 0.371180 7_4:-56863586 Msa1165020:CDS
GAATATCCCGTTGCTTCTTT+GGG 0.376906 7_4:-56863484 Msa1165020:intron
GCGCAGGTTGACGGCATTAT+GGG 0.391190 7_4:-56862548 Msa1165020:CDS
GTGACAAATTTGGTTTGGTT+TGG 0.404272 7_4:-56863693 Msa1165020:five_prime_UTR
ATTGAGTGACAAATTTGGTT+TGG 0.407348 7_4:-56863698 Msa1165020:five_prime_UTR
TTACTATATGTACAGATTGT+GGG 0.418392 7_4:-56862804 Msa1165020:intron
AGTAGTGATAGTTTATTAGT+TGG 0.424734 7_4:+56862461 None:intergenic
GAAGTAGAAAAGTGCAGCAT+AGG 0.426789 7_4:-56862625 Msa1165020:CDS
CAGAGCTTGTTGAAGAGCGC+AGG 0.429709 7_4:-56862564 Msa1165020:CDS
TTTGGTTTGGTTTGCAATGA+GGG 0.434025 7_4:-56863680 Msa1165020:exon
AAGAACGAGCTCGTGATTAT+GGG 0.441049 7_4:-56862525 Msa1165020:CDS
GGTCTGTTCCAAAGTCTCAT+TGG 0.443502 7_4:+56862696 None:intergenic
TTTAAGACCCTGCCGTTAAG+TGG 0.447186 7_4:+56862334 None:intergenic
AATTGTAGCCAATGAGACTT+TGG 0.447499 7_4:-56862704 Msa1165020:CDS
GTGATTATGGGTGGAAGGAC+AGG 0.457853 7_4:-56862513 Msa1165020:CDS
AACTTACCCAAAGAAGCAAC+GGG 0.499220 7_4:+56863478 None:intergenic
TGTTGAAGAGCGCAGGTTGA+CGG 0.500440 7_4:-56862557 Msa1165020:CDS
GGTGAATTCCACTTAACGGC+AGG 0.511432 7_4:-56862342 Msa1165020:three_prime_UTR
AAGTAGAAAAGTGCAGCATA+GGG 0.518644 7_4:-56862624 Msa1165020:CDS
TTGTGATTATTTAACCATGT+AGG 0.521426 7_4:-56862393 Msa1165020:three_prime_UTR
GTTTGGTTTGGTTTGCAATG+AGG 0.525335 7_4:-56863681 Msa1165020:exon
TTCTTCAAACGCCAGCGAGA+TGG 0.541960 7_4:+56863559 None:intergenic
AGAAAAGTGCAGCATAGGGA+TGG 0.556983 7_4:-56862620 Msa1165020:CDS
CAATCATGACATTGAGGACA+AGG 0.561176 7_4:+56863532 None:intergenic
CAATTGGACCACCATCTCGC+TGG 0.563845 7_4:-56863570 Msa1165020:CDS
TGGAAACTTGTGAAGAAGCA+AGG 0.573605 7_4:-56862600 Msa1165020:CDS
TTCAAACGCCAGCGAGATGG+TGG 0.580007 7_4:+56863562 None:intergenic
AGAAGCAAGGGAGAGAGCAG+AGG 0.585238 7_4:-56862587 Msa1165020:CDS
GAGACATGATCCAACAATGA+AGG 0.590741 7_4:-56862782 Msa1165020:CDS
AGCTCGTGATTATGGGTGGA+AGG 0.596678 7_4:-56862518 Msa1165020:CDS
AAAGATTAGTAATAAATGCA+AGG 0.596837 7_4:-56862672 Msa1165020:CDS
TCCTCTGATAGCATTGTCAG+TGG 0.601328 7_4:+56862742 None:intergenic
AAACTTACCCAAAGAAGCAA+CGG 0.604283 7_4:+56863477 None:intergenic
AACGAGCTCGTGATTATGGG+TGG 0.612321 7_4:-56862522 Msa1165020:CDS
GTGAATTCCACTTAACGGCA+GGG 0.613965 7_4:-56862341 Msa1165020:three_prime_UTR
GGAAACTTGTGAAGAAGCAA+GGG 0.615984 7_4:-56862599 Msa1165020:CDS
CAGTAACAATCATGACATTG+AGG 0.634830 7_4:+56863526 None:intergenic
TCCACTGACAATGCTATCAG+AGG 0.642154 7_4:-56862743 Msa1165020:CDS
ATATTCCAGTGAGGCAAGTG+AGG 0.642772 7_4:+56863501 None:intergenic
ACATGATCCAACAATGAAGG+AGG 0.647322 7_4:-56862779 Msa1165020:CDS
GTGGTCCAATTGTGAACACA+AGG 0.658093 7_4:+56863581 None:intergenic
AATGAAGGAGGAAATGAACA+AGG 0.667487 7_4:-56862767 Msa1165020:CDS
AGCAACGGGATATTCCAGTG+AGG 0.784097 7_4:+56863492 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAAATTTTACTTAAAG+TGG + chr7_4:56863129-56863148 None:intergenic 10.0%
!!! AAAATACTAATTTAGTTTTA+AGG + chr7_4:56863713-56863732 None:intergenic 10.0%
!!! TTAAAACTAAATTAGTATTT+TGG - chr7_4:56863712-56863731 Msa1165020:five_prime_UTR 10.0%
!!! ATTATAAATTTTACTTAAAG+TGG + chr7_4:56863129-56863148 None:intergenic 10.0%
!!! AAAATACTAATTTAGTTTTA+AGG + chr7_4:56863713-56863732 None:intergenic 10.0%
!!! TTAAAACTAAATTAGTATTT+TGG - chr7_4:56863712-56863731 Msa1165020:five_prime_UTR 10.0%
!! TAGTAATTAGAAAAATTCAC+TGG - chr7_4:56862733-56862752 Msa1165020:CDS 20.0%
!! CAATTCAATTCATATGTATT+AGG - chr7_4:56862820-56862839 Msa1165020:intron 20.0%
!! TAGGTTTATATATTAATTCG+TGG - chr7_4:56862839-56862858 Msa1165020:intron 20.0%
!! AGAAATGCAAAACATTTAAA+TGG + chr7_4:56863040-56863059 None:intergenic 20.0%
!!! TGCATTTATTACTAATCTTT+TGG + chr7_4:56863403-56863422 None:intergenic 20.0%
!! AAAGATTAGTAATAAATGCA+AGG - chr7_4:56863403-56863422 Msa1165020:intron 20.0%
!! TAGTAATTAGAAAAATTCAC+TGG - chr7_4:56862733-56862752 Msa1165020:CDS 20.0%
!! CAATTCAATTCATATGTATT+AGG - chr7_4:56862820-56862839 Msa1165020:intron 20.0%
!! TAGGTTTATATATTAATTCG+TGG - chr7_4:56862839-56862858 Msa1165020:intron 20.0%
!! AGAAATGCAAAACATTTAAA+TGG + chr7_4:56863040-56863059 None:intergenic 20.0%
!!! TGCATTTATTACTAATCTTT+TGG + chr7_4:56863403-56863422 None:intergenic 20.0%
!! AAAGATTAGTAATAAATGCA+AGG - chr7_4:56863403-56863422 Msa1165020:intron 20.0%
! TTTGTAGAGTAAGTGAAAAA+GGG + chr7_4:56862624-56862643 None:intergenic 25.0%
! ATTTGTAGAGTAAGTGAAAA+AGG + chr7_4:56862625-56862644 None:intergenic 25.0%
!!! CATCATTTTTCAAACACAAA+GGG + chr7_4:56862710-56862729 None:intergenic 25.0%
! ATTGAATAACCAAAACATTC+TGG + chr7_4:56862804-56862823 None:intergenic 25.0%
! TTTACTATATGTACAGATTG+TGG - chr7_4:56863270-56863289 Msa1165020:intron 25.0%
! TTACTATATGTACAGATTGT+GGG - chr7_4:56863271-56863290 Msa1165020:intron 25.0%
! AGTAGTGATAGTTTATTAGT+TGG + chr7_4:56863617-56863636 None:intergenic 25.0%
! TTGTGATTATTTAACCATGT+AGG - chr7_4:56863682-56863701 Msa1165020:exon 25.0%
! TTTGTAGAGTAAGTGAAAAA+GGG + chr7_4:56862624-56862643 None:intergenic 25.0%
! ATTTGTAGAGTAAGTGAAAA+AGG + chr7_4:56862625-56862644 None:intergenic 25.0%
!!! CATCATTTTTCAAACACAAA+GGG + chr7_4:56862710-56862729 None:intergenic 25.0%
! ATTGAATAACCAAAACATTC+TGG + chr7_4:56862804-56862823 None:intergenic 25.0%
! TTTACTATATGTACAGATTG+TGG - chr7_4:56863270-56863289 Msa1165020:intron 25.0%
! TTACTATATGTACAGATTGT+GGG - chr7_4:56863271-56863290 Msa1165020:intron 25.0%
! AGTAGTGATAGTTTATTAGT+TGG + chr7_4:56863617-56863636 None:intergenic 25.0%
! TTGTGATTATTTAACCATGT+AGG - chr7_4:56863682-56863701 Msa1165020:exon 25.0%
GTTCAATTGAGTGACAAATT+TGG - chr7_4:56862372-56862391 Msa1165020:three_prime_UTR 30.0%
ATTGAGTGACAAATTTGGTT+TGG - chr7_4:56862377-56862396 Msa1165020:three_prime_UTR 30.0%
!!! TTCTTATGGTGTTTTGAAGT+GGG - chr7_4:56862446-56862465 Msa1165020:three_prime_UTR 30.0%
ACTCTACAAATTAACAGACT+TGG - chr7_4:56862634-56862653 Msa1165020:CDS 30.0%
ACAAGTTTGATTAGATGTAC+AGG - chr7_4:56862673-56862692 Msa1165020:CDS 30.0%
!! GCATCATTTTTCAAACACAA+AGG + chr7_4:56862711-56862730 None:intergenic 30.0%
CTTTGTTCTGATTCTCAAAA+AGG + chr7_4:56862762-56862781 None:intergenic 30.0%
! ATGTATGTTTTGTATCACAG+TGG + chr7_4:56862930-56862949 None:intergenic 30.0%
TGATACAAAACATACATCGA+AGG - chr7_4:56862933-56862952 Msa1165020:intron 30.0%
CTAAAAATGAGAACAGAATG+AGG + chr7_4:56863189-56863208 None:intergenic 30.0%
ATAATGTCCAAAAGACAACA+TGG + chr7_4:56863232-56863251 None:intergenic 30.0%
! TTTTGGTGAATTCCACTTAA+CGG - chr7_4:56863729-56863748 Msa1165020:five_prime_UTR 30.0%
GTTCAATTGAGTGACAAATT+TGG - chr7_4:56862372-56862391 Msa1165020:three_prime_UTR 30.0%
ATTGAGTGACAAATTTGGTT+TGG - chr7_4:56862377-56862396 Msa1165020:three_prime_UTR 30.0%
!!! TTCTTATGGTGTTTTGAAGT+GGG - chr7_4:56862446-56862465 Msa1165020:three_prime_UTR 30.0%
ACTCTACAAATTAACAGACT+TGG - chr7_4:56862634-56862653 Msa1165020:CDS 30.0%
ACAAGTTTGATTAGATGTAC+AGG - chr7_4:56862673-56862692 Msa1165020:CDS 30.0%
!! GCATCATTTTTCAAACACAA+AGG + chr7_4:56862711-56862730 None:intergenic 30.0%
CTTTGTTCTGATTCTCAAAA+AGG + chr7_4:56862762-56862781 None:intergenic 30.0%
! ATGTATGTTTTGTATCACAG+TGG + chr7_4:56862930-56862949 None:intergenic 30.0%
TGATACAAAACATACATCGA+AGG - chr7_4:56862933-56862952 Msa1165020:intron 30.0%
CTAAAAATGAGAACAGAATG+AGG + chr7_4:56863189-56863208 None:intergenic 30.0%
ATAATGTCCAAAAGACAACA+TGG + chr7_4:56863232-56863251 None:intergenic 30.0%
! TTTTGGTGAATTCCACTTAA+CGG - chr7_4:56863729-56863748 Msa1165020:five_prime_UTR 30.0%
!! GTGACAAATTTGGTTTGGTT+TGG - chr7_4:56862382-56862401 Msa1165020:three_prime_UTR 35.0%
!! TTTGGTTTGGTTTGCAATGA+GGG - chr7_4:56862395-56862414 Msa1165020:three_prime_UTR 35.0%
GAGCAAAGACAATGTTCTTA+TGG - chr7_4:56862432-56862451 Msa1165020:three_prime_UTR 35.0%
!!! GTTCTTATGGTGTTTTGAAG+TGG - chr7_4:56862445-56862464 Msa1165020:three_prime_UTR 35.0%
!!! TTTTGAAGTGGGTTTTGGTT+TGG - chr7_4:56862457-56862476 Msa1165020:three_prime_UTR 35.0%
TGTTACCTTGTGTTCACAAT+TGG - chr7_4:56862489-56862508 Msa1165020:exon 35.0%
CAGTAACAATCATGACATTG+AGG + chr7_4:56862552-56862571 None:intergenic 35.0%
AAACTTACCCAAAGAAGCAA+CGG + chr7_4:56862601-56862620 None:intergenic 35.0%
! TCTATCTAGCCAGAATGTTT+TGG - chr7_4:56862792-56862811 Msa1165020:CDS 35.0%
!! CATAGCTAGCTTTGTTTATG+TGG - chr7_4:56863065-56863084 Msa1165020:intron 35.0%
TTCATTTCCTCCTTCATTGT+TGG + chr7_4:56863306-56863325 None:intergenic 35.0%
AATGAAGGAGGAAATGAACA+AGG - chr7_4:56863308-56863327 Msa1165020:intron 35.0%
AATTGTAGCCAATGAGACTT+TGG - chr7_4:56863371-56863390 Msa1165020:intron 35.0%
! AAGTAGAAAAGTGCAGCATA+GGG - chr7_4:56863451-56863470 Msa1165020:intron 35.0%
!! GTGACAAATTTGGTTTGGTT+TGG - chr7_4:56862382-56862401 Msa1165020:three_prime_UTR 35.0%
!! TTTGGTTTGGTTTGCAATGA+GGG - chr7_4:56862395-56862414 Msa1165020:three_prime_UTR 35.0%
GAGCAAAGACAATGTTCTTA+TGG - chr7_4:56862432-56862451 Msa1165020:three_prime_UTR 35.0%
!!! GTTCTTATGGTGTTTTGAAG+TGG - chr7_4:56862445-56862464 Msa1165020:three_prime_UTR 35.0%
!!! TTTTGAAGTGGGTTTTGGTT+TGG - chr7_4:56862457-56862476 Msa1165020:three_prime_UTR 35.0%
TGTTACCTTGTGTTCACAAT+TGG - chr7_4:56862489-56862508 Msa1165020:exon 35.0%
CAGTAACAATCATGACATTG+AGG + chr7_4:56862552-56862571 None:intergenic 35.0%
AAACTTACCCAAAGAAGCAA+CGG + chr7_4:56862601-56862620 None:intergenic 35.0%
! TCTATCTAGCCAGAATGTTT+TGG - chr7_4:56862792-56862811 Msa1165020:CDS 35.0%
!! CATAGCTAGCTTTGTTTATG+TGG - chr7_4:56863065-56863084 Msa1165020:intron 35.0%
TTCATTTCCTCCTTCATTGT+TGG + chr7_4:56863306-56863325 None:intergenic 35.0%
AATGAAGGAGGAAATGAACA+AGG - chr7_4:56863308-56863327 Msa1165020:intron 35.0%
AATTGTAGCCAATGAGACTT+TGG - chr7_4:56863371-56863390 Msa1165020:intron 35.0%
! AAGTAGAAAAGTGCAGCATA+GGG - chr7_4:56863451-56863470 Msa1165020:intron 35.0%
!! GTTTGGTTTGGTTTGCAATG+AGG - chr7_4:56862394-56862413 Msa1165020:three_prime_UTR 40.0%
!!! TGGTGTTTTGAAGTGGGTTT+TGG - chr7_4:56862452-56862471 Msa1165020:three_prime_UTR 40.0%
CAATCATGACATTGAGGACA+AGG + chr7_4:56862546-56862565 None:intergenic 40.0%
GAATATCCCGTTGCTTCTTT+GGG - chr7_4:56862591-56862610 Msa1165020:CDS 40.0%
AACTTACCCAAAGAAGCAAC+GGG + chr7_4:56862600-56862619 None:intergenic 40.0%
!! TTGCCTTGTTTAGCTCAACA+CGG - chr7_4:56862958-56862977 Msa1165020:intron 40.0%
! TTGTTTAGCTCAACACGGTA+CGG - chr7_4:56862963-56862982 Msa1165020:intron 40.0%
! TTCACCACCATGTTGTCTTT+TGG - chr7_4:56863222-56863241 Msa1165020:intron 40.0%
ATGTCCAAAAGACAACATGG+TGG + chr7_4:56863229-56863248 None:intergenic 40.0%
GAGACATGATCCAACAATGA+AGG - chr7_4:56863293-56863312 Msa1165020:intron 40.0%
ACATGATCCAACAATGAAGG+AGG - chr7_4:56863296-56863315 Msa1165020:intron 40.0%
! GAAGTAGAAAAGTGCAGCAT+AGG - chr7_4:56863450-56863469 Msa1165020:intron 40.0%
TGGAAACTTGTGAAGAAGCA+AGG - chr7_4:56863475-56863494 Msa1165020:intron 40.0%
GGAAACTTGTGAAGAAGCAA+GGG - chr7_4:56863476-56863495 Msa1165020:intron 40.0%
AAGAACGAGCTCGTGATTAT+GGG - chr7_4:56863550-56863569 Msa1165020:CDS 40.0%
!!! GTTTTAAGGCATAGCCTACA+TGG + chr7_4:56863699-56863718 None:intergenic 40.0%
!! GTTTGGTTTGGTTTGCAATG+AGG - chr7_4:56862394-56862413 Msa1165020:three_prime_UTR 40.0%
!!! TGGTGTTTTGAAGTGGGTTT+TGG - chr7_4:56862452-56862471 Msa1165020:three_prime_UTR 40.0%
CAATCATGACATTGAGGACA+AGG + chr7_4:56862546-56862565 None:intergenic 40.0%
GAATATCCCGTTGCTTCTTT+GGG - chr7_4:56862591-56862610 Msa1165020:CDS 40.0%
AACTTACCCAAAGAAGCAAC+GGG + chr7_4:56862600-56862619 None:intergenic 40.0%
!! TTGCCTTGTTTAGCTCAACA+CGG - chr7_4:56862958-56862977 Msa1165020:intron 40.0%
! TTGTTTAGCTCAACACGGTA+CGG - chr7_4:56862963-56862982 Msa1165020:intron 40.0%
! TTCACCACCATGTTGTCTTT+TGG - chr7_4:56863222-56863241 Msa1165020:intron 40.0%
ATGTCCAAAAGACAACATGG+TGG + chr7_4:56863229-56863248 None:intergenic 40.0%
GAGACATGATCCAACAATGA+AGG - chr7_4:56863293-56863312 Msa1165020:intron 40.0%
ACATGATCCAACAATGAAGG+AGG - chr7_4:56863296-56863315 Msa1165020:intron 40.0%
! GAAGTAGAAAAGTGCAGCAT+AGG - chr7_4:56863450-56863469 Msa1165020:intron 40.0%
TGGAAACTTGTGAAGAAGCA+AGG - chr7_4:56863475-56863494 Msa1165020:intron 40.0%
GGAAACTTGTGAAGAAGCAA+GGG - chr7_4:56863476-56863495 Msa1165020:intron 40.0%
AAGAACGAGCTCGTGATTAT+GGG - chr7_4:56863550-56863569 Msa1165020:CDS 40.0%
!!! GTTTTAAGGCATAGCCTACA+TGG + chr7_4:56863699-56863718 None:intergenic 40.0%
GTGGTCCAATTGTGAACACA+AGG + chr7_4:56862497-56862516 None:intergenic 45.0%
ATATTCCAGTGAGGCAAGTG+AGG + chr7_4:56862577-56862596 None:intergenic 45.0%
GGAATATCCCGTTGCTTCTT+TGG - chr7_4:56862590-56862609 Msa1165020:CDS 45.0%
GTACCGTGTTGAGCTAAACA+AGG + chr7_4:56862964-56862983 None:intergenic 45.0%
! TGTTTAGCTCAACACGGTAC+GGG - chr7_4:56862964-56862983 Msa1165020:intron 45.0%
TCCACTGACAATGCTATCAG+AGG - chr7_4:56863332-56863351 Msa1165020:intron 45.0%
TCCTCTGATAGCATTGTCAG+TGG + chr7_4:56863336-56863355 None:intergenic 45.0%
GGTCTGTTCCAAAGTCTCAT+TGG + chr7_4:56863382-56863401 None:intergenic 45.0%
! AGAAAAGTGCAGCATAGGGA+TGG - chr7_4:56863455-56863474 Msa1165020:intron 45.0%
GAAGAACGAGCTCGTGATTA+TGG - chr7_4:56863549-56863568 Msa1165020:CDS 45.0%
GTGAATTCCACTTAACGGCA+GGG - chr7_4:56863734-56863753 Msa1165020:five_prime_UTR 45.0%
GTGGTCCAATTGTGAACACA+AGG + chr7_4:56862497-56862516 None:intergenic 45.0%
ATATTCCAGTGAGGCAAGTG+AGG + chr7_4:56862577-56862596 None:intergenic 45.0%
GGAATATCCCGTTGCTTCTT+TGG - chr7_4:56862590-56862609 Msa1165020:CDS 45.0%
GTACCGTGTTGAGCTAAACA+AGG + chr7_4:56862964-56862983 None:intergenic 45.0%
! TGTTTAGCTCAACACGGTAC+GGG - chr7_4:56862964-56862983 Msa1165020:intron 45.0%
TCCACTGACAATGCTATCAG+AGG - chr7_4:56863332-56863351 Msa1165020:intron 45.0%
TCCTCTGATAGCATTGTCAG+TGG + chr7_4:56863336-56863355 None:intergenic 45.0%
GGTCTGTTCCAAAGTCTCAT+TGG + chr7_4:56863382-56863401 None:intergenic 45.0%
! AGAAAAGTGCAGCATAGGGA+TGG - chr7_4:56863455-56863474 Msa1165020:intron 45.0%
GAAGAACGAGCTCGTGATTA+TGG - chr7_4:56863549-56863568 Msa1165020:CDS 45.0%
GTGAATTCCACTTAACGGCA+GGG - chr7_4:56863734-56863753 Msa1165020:five_prime_UTR 45.0%
TTCTTCAAACGCCAGCGAGA+TGG + chr7_4:56862519-56862538 None:intergenic 50.0%
AGCAACGGGATATTCCAGTG+AGG + chr7_4:56862586-56862605 None:intergenic 50.0%
! GTTTAGCTCAACACGGTACG+GGG - chr7_4:56862965-56862984 Msa1165020:intron 50.0%
! TGTTGAAGAGCGCAGGTTGA+CGG - chr7_4:56863518-56863537 Msa1165020:CDS 50.0%
AACGAGCTCGTGATTATGGG+TGG - chr7_4:56863553-56863572 Msa1165020:CDS 50.0%
AGCTCGTGATTATGGGTGGA+AGG - chr7_4:56863557-56863576 Msa1165020:CDS 50.0%
GTGATTATGGGTGGAAGGAC+AGG - chr7_4:56863562-56863581 Msa1165020:CDS 50.0%
GGTGAATTCCACTTAACGGC+AGG - chr7_4:56863733-56863752 Msa1165020:five_prime_UTR 50.0%
TTCTTCAAACGCCAGCGAGA+TGG + chr7_4:56862519-56862538 None:intergenic 50.0%
AGCAACGGGATATTCCAGTG+AGG + chr7_4:56862586-56862605 None:intergenic 50.0%
! GTTTAGCTCAACACGGTACG+GGG - chr7_4:56862965-56862984 Msa1165020:intron 50.0%
! TGTTGAAGAGCGCAGGTTGA+CGG - chr7_4:56863518-56863537 Msa1165020:CDS 50.0%
AACGAGCTCGTGATTATGGG+TGG - chr7_4:56863553-56863572 Msa1165020:CDS 50.0%
AGCTCGTGATTATGGGTGGA+AGG - chr7_4:56863557-56863576 Msa1165020:CDS 50.0%
GTGATTATGGGTGGAAGGAC+AGG - chr7_4:56863562-56863581 Msa1165020:CDS 50.0%
GGTGAATTCCACTTAACGGC+AGG - chr7_4:56863733-56863752 Msa1165020:five_prime_UTR 50.0%
CAATTGGACCACCATCTCGC+TGG - chr7_4:56862505-56862524 Msa1165020:exon 55.0%
TTCAAACGCCAGCGAGATGG+TGG + chr7_4:56862516-56862535 None:intergenic 55.0%
CTGTTCCTCACTTGCCTCAC+TGG - chr7_4:56862569-56862588 Msa1165020:CDS 55.0%
AGAAGCAAGGGAGAGAGCAG+AGG - chr7_4:56863488-56863507 Msa1165020:CDS 55.0%
!! CAGAGCTTGTTGAAGAGCGC+AGG - chr7_4:56863511-56863530 Msa1165020:CDS 55.0%
! AGCGCAGGTTGACGGCATTA+TGG - chr7_4:56863526-56863545 Msa1165020:CDS 55.0%
! GCGCAGGTTGACGGCATTAT+GGG - chr7_4:56863527-56863546 Msa1165020:CDS 55.0%
CAATTGGACCACCATCTCGC+TGG - chr7_4:56862505-56862524 Msa1165020:exon 55.0%
TTCAAACGCCAGCGAGATGG+TGG + chr7_4:56862516-56862535 None:intergenic 55.0%
CTGTTCCTCACTTGCCTCAC+TGG - chr7_4:56862569-56862588 Msa1165020:CDS 55.0%
AGAAGCAAGGGAGAGAGCAG+AGG - chr7_4:56863488-56863507 Msa1165020:CDS 55.0%
!! CAGAGCTTGTTGAAGAGCGC+AGG - chr7_4:56863511-56863530 Msa1165020:CDS 55.0%
! AGCGCAGGTTGACGGCATTA+TGG - chr7_4:56863526-56863545 Msa1165020:CDS 55.0%
! GCGCAGGTTGACGGCATTAT+GGG - chr7_4:56863527-56863546 Msa1165020:CDS 55.0%
GCTCAACACGGTACGGGGAT+TGG - chr7_4:56862970-56862989 Msa1165020:intron 60.0%
GCTCAACACGGTACGGGGAT+TGG - chr7_4:56862970-56862989 Msa1165020:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 56862335 56863762 56862335 ID=Msa1165020;Name=Msa1165020
chr7_4 mRNA 56862335 56863762 56862335 ID=Msa1165020-mRNA-1;Parent=Msa1165020;Name=Msa1165020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=76|1|1|1|1|1|2|172|168
chr7_4 exon 56862335 56862811 56862335 ID=Msa1165020-mRNA-1:exon:17030;Parent=Msa1165020-mRNA-1
chr7_4 exon 56863485 56863762 56863485 ID=Msa1165020-mRNA-1:exon:17029;Parent=Msa1165020-mRNA-1
chr7_4 five_prime_UTR 56863687 56863762 56863687 ID=Msa1165020-mRNA-1:five_prime_utr;Parent=Msa1165020-mRNA-1
chr7_4 CDS 56863485 56863686 56863485 ID=Msa1165020-mRNA-1:cds;Parent=Msa1165020-mRNA-1
chr7_4 CDS 56862507 56862811 56862507 ID=Msa1165020-mRNA-1:cds;Parent=Msa1165020-mRNA-1
chr7_4 three_prime_UTR 56862335 56862506 56862335 ID=Msa1165020-mRNA-1:three_prime_utr;Parent=Msa1165020-mRNA-1
Gene Sequence

>Msa1165020

ATGAGGGTTCATCATCAAAATGAGCAAAGACAATGTTCTTATGGTGTTTTGAAGTGGGTTTTGGTTTGGATGAGTGTGTGTTACCTTGTGTTCACAATTGGACCACCATCTCGCTGGCGTTTGAAGAAATCTCCTTGTCCTCAATGTCATGATTGTTACTGTTCCTCACTTGCCTCACTGGAATATCCCGTTGCTTCTTTGGATTGTGGGAGACATGATCCAACAATGAAGGAGGAAATGAACAAGGATCCACTGACAATGCTATCAGAGGAGTTAAAGTTGCAGAAAATTGTAGCCAATGAGACTTTGGAACAGACCAAAAGATTAGTAATAAATGCAAGGAATACTTTCTCTCATTATAAAAAAGAAGTAGAAAAGTGCAGCATAGGGATGGAAACTTGTGAAGAAGCAAGGGAGAGAGCAGAGGCAGAGCTTGTTGAAGAGCGCAGGTTGACGGCATTATGGGAAGAACGAGCTCGTGATTATGGGTGGAAGGACAGGAGATGA
Protein sequence

>Msa1165020

MRVHHQNEQRQCSYGVLKWVLVWMSVCYLVFTIGPPSRWRLKKSPCPQCHDCYCSSLASLEYPVASLDCGRHDPTMKEEMNKDPLTMLSEELKLQKIVANETLEQTKRLVINARNTFSHYKKEVEKCSIGMETCEEARERAEAELVEERRLTALWEERARDYGWKDRR*