AlfalfaGEDB | Msa1424060

M. sativa cultivar zhongmu-4 / Msa1424060

KEGG: K20729
GO: GO:0003002 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009987 GO:0010016 GO:0010052 GO:0010103 GO:0010374 GO:0010375 GO:0030154 GO:0032501 GO:0032502 GO:0048367 GO:0048519 GO:0048580 GO:0048581 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0065007 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:2000026 GO:2000038 GO:2000122
PFAM: PF17181.7

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424060	sp\|Q8S8I4\|EPF1_ARATH	85.714	63	9	0	63	125	42	104	1.61e-34	118

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424060	A0A072VBC6	94.400	125	5	1	1	125	1	123	3.91e-81	243

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0065390	Msa1424060	0.802236	6.173185e-49	-8.615850e-47
Msa0094060	Msa1424060	0.810681	1.021332e-50	-8.615850e-47
Msa0103780	Msa1424060	0.806250	9.009501e-50	-8.615850e-47
Msa0110710	Msa1424060	0.806424	8.282547e-50	-8.615850e-47
Msa1354040	Msa1424060	0.807139	5.848076e-50	-8.615850e-47
Msa1375400	Msa1424060	0.809198	2.129989e-50	-8.615850e-47
Msa1375630	Msa1424060	0.810341	1.209860e-50	-8.615850e-47
Msa1376530	Msa1424060	0.807741	4.358370e-50	-8.615850e-47
Msa1424060	Msa1424240	0.806223	9.132170e-50	-8.615850e-47
Msa1424060	Msa1429390	0.802902	4.499645e-49	-8.615850e-47
Msa1424060	Msa1437150	0.821396	4.127992e-53	-8.615850e-47
Msa1424060	Msa1442030	0.800847	1.189241e-48	-8.615850e-47
Msa0703760	Msa1424060	0.810414	1.166756e-50	-8.615850e-47
Msa0734430	Msa1424060	0.811453	6.949427e-51	-8.615850e-47
Msa0738970	Msa1424060	0.821745	3.428222e-53	-8.615850e-47
Msa0803130	Msa1424060	0.802652	5.069163e-49	-8.615850e-47
Msa0804110	Msa1424060	0.811400	7.135728e-51	-8.615850e-47
Msa0819470	Msa1424060	0.801154	1.029560e-48	-8.615850e-47
Msa0823300	Msa1424060	0.800154	1.646019e-48	-8.615850e-47
Msa0841490	Msa1424060	0.801955	7.053782e-49	-8.615850e-47
Msa0135980	Msa1424060	0.802547	5.327573e-49	-8.615850e-47
Msa0150210	Msa1424060	0.800713	1.266855e-48	-8.615850e-47
Msa0185550	Msa1424060	0.809651	1.703217e-50	-8.615850e-47
Msa0206970	Msa1424060	0.808181	3.514326e-50	-8.615850e-47
Msa0207180	Msa1424060	0.802929	4.441883e-49	-8.615850e-47
Msa0230400	Msa1424060	0.815692	8.115097e-52	-8.615850e-47
Msa1232010	Msa1424060	0.826116	3.236820e-54	-8.615850e-47
Msa1232860	Msa1424060	0.801529	8.626512e-49	-8.615850e-47
Msa1254210	Msa1424060	0.802680	5.000745e-49	-8.615850e-47
Msa1274740	Msa1424060	0.828570	8.352241e-55	-8.615850e-47
Msa1306540	Msa1424060	0.802980	4.336949e-49	-8.615850e-47
Msa1312730	Msa1424060	0.834402	3.060853e-56	-8.615850e-47
Msa0380420	Msa1424060	0.816833	4.510010e-52	-8.615850e-47
Msa0382290	Msa1424060	0.806267	8.938982e-50	-8.615850e-47
Msa0392410	Msa1424060	0.801649	8.148884e-49	-8.615850e-47
Msa0392450	Msa1424060	0.810917	9.082072e-51	-8.615850e-47
Msa0392480	Msa1424060	0.800759	1.239409e-48	-8.615850e-47
Msa0413180	Msa1424060	0.805834	1.102340e-49	-8.615850e-47
Msa0421790	Msa1424060	0.814743	1.318851e-51	-8.615850e-47
Msa0448160	Msa1424060	0.805024	1.629998e-49	-8.615850e-47
Msa0467890	Msa1424060	0.808640	2.804176e-50	-8.615850e-47
Msa0476150	Msa1424060	0.806779	6.970875e-50	-8.615850e-47
Msa0988030	Msa1424060	0.803458	3.453416e-49	-8.615850e-47
Msa1027790	Msa1424060	0.801086	1.062911e-48	-8.615850e-47
Msa0247310	Msa1424060	0.803042	4.210694e-49	-8.615850e-47
Msa0261740	Msa1424060	0.961420	1.108606e-119	-8.615850e-47
Msa0265780	Msa1424060	0.809146	2.185184e-50	-8.615850e-47
Msa0274780	Msa1424060	0.804263	2.349169e-49	-8.615850e-47
Msa0299620	Msa1424060	0.986742	8.138494e-168	-8.615850e-47
Msa0303780	Msa1424060	0.809122	2.211989e-50	-8.615850e-47
Msa0315480	Msa1424060	0.807299	5.409918e-50	-8.615850e-47
Msa0337100	Msa1424060	0.942680	4.680693e-102	-8.615850e-47
Msa1180410	Msa1424060	0.813818	2.111438e-51	-8.615850e-47
Msa0487560	Msa1424060	0.813654	2.294016e-51	-8.615850e-47
Msa0487620	Msa1424060	0.820653	6.123503e-53	-8.615850e-47
Msa0510230	Msa1424060	0.817249	3.636761e-52	-8.615850e-47
Msa0567290	Msa1424060	0.807835	4.162846e-50	-8.615850e-47
Msa0859390	Msa1424060	0.821639	3.627877e-53	-8.615850e-47
Msa0930330	Msa1424060	0.807080	6.020665e-50	-8.615850e-47
Msa0946650	Msa1424060	0.810838	9.444235e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424060	MtrunA17_Chr2g0324101	100.000	55	0	0	71	125	1	55	9.26e-35	114
Msa1424060	MtrunA17_Chr2g0311361	59.615	52	19	1	74	125	47	96	7.30e-14	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424060	AT2G20875.1	85.714	63	9	0	63	125	42	104	1.64e-35	118
Msa1424060	AT1G34245.1	36.752	117	62	3	12	125	10	117	1.58e-15	67.8
Msa1424060	AT1G71866.2	36.220	127	62	5	10	125	15	133	8.46e-15	66.2
Msa1424060	AT1G71866.1	50.000	54	24	1	72	125	119	169	1.41e-13	63.9

Find 44 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTATTATAACCAATTCTA+TGG	0.189428	tig0025614:-49751	None:intergenic
CGGTTTCCTAGCAATGAAAA+TGG	0.264943	tig0025614:+49906	Msa1424060:three_prime_UTR
AACCATGATGAAGAGGAATT+TGG	0.314180	tig0025614:+48137	Msa1424060:exon
TTGGAATATAAAGTAAAATA+AGG	0.344189	tig0025614:-48179	None:intergenic
ACAAAATAGATGAAAGTAAA+GGG	0.369736	tig0025614:+49509	Msa1424060:CDS
AGTAGCTGGTTCGAGGTTGC+CGG	0.386785	tig0025614:+49594	Msa1424060:CDS
GTTATGGCACATGCATTTGT+AGG	0.393759	tig0025614:-49713	None:intergenic
CTCACCATGCAGATTGGTTA+TGG	0.400298	tig0025614:+49642	Msa1424060:CDS
TCATGCTTCAATTTCTAACT+TGG	0.405231	tig0025614:+49781	Msa1424060:three_prime_UTR
CCTATCCTGTCCCATAGAAT+TGG	0.421919	tig0025614:+49740	Msa1424060:exon
TTCCAATCATCACCTCTTCA+AGG	0.450570	tig0025614:+48196	Msa1424060:CDS
CACATGCATTTGTAGGCCAT+TGG	0.478400	tig0025614:-49706	None:intergenic
GTTGTCAAACATGGTGGAAA+CGG	0.478632	tig0025614:+49886	Msa1424060:three_prime_UTR
CCAGCTACTTGCATCGTGTC+CGG	0.481240	tig0025614:-49580	None:intergenic
TGCAATTTCAAAATGTTGAG+TGG	0.489806	tig0025614:-48105	None:intergenic
GGACAGGATAGGACTTGTTA+TGG	0.495900	tig0025614:-49729	None:intergenic
TGCCTTGAAGAGGTGATGAT+TGG	0.500190	tig0025614:-48198	None:intergenic
AGAAATTAACCATGATGAAG+AGG	0.501659	tig0025614:+48130	Msa1424060:exon
AACAAAATAGATGAAAGTAA+AGG	0.501713	tig0025614:+49508	Msa1424060:CDS
TATAACCAATTCTATGGGAC+AGG	0.503159	tig0025614:-49745	None:intergenic
CCGGACACGATGCAAGTAGC+TGG	0.504387	tig0025614:+49580	Msa1424060:CDS
TTTCTCTCTGTTGTCAAACA+TGG	0.507488	tig0025614:+49877	Msa1424060:three_prime_UTR
GACCAAATTCCTCTTCATCA+TGG	0.510199	tig0025614:-48139	None:intergenic
ATCAAAACATACGCGGCGGC+CGG	0.515180	tig0025614:+49561	Msa1424060:CDS
GGGCAAAACAAAATCTGCAT+TGG	0.522159	tig0025614:+49530	Msa1424060:CDS
TCTATTATAACCAATTCTAT+GGG	0.530668	tig0025614:-49750	None:intergenic
ATCATGCTCACCATGCAGAT+TGG	0.536692	tig0025614:+49636	Msa1424060:CDS
CGATGCAAGTAGCTGGTTCG+AGG	0.546321	tig0025614:+49587	Msa1424060:CDS
CCGCATGCATGAGAACAATC+CGG	0.553599	tig0025614:-49613	None:intergenic
GGACATTCAGATCTTGATGT+AGG	0.560423	tig0025614:+49484	Msa1424060:CDS
AAACAAAATCTGCATTGGAA+AGG	0.567974	tig0025614:+49535	Msa1424060:CDS
GAACTAAGAAGAACATCTCA+AGG	0.571635	tig0025614:+48074	Msa1424060:five_prime_UTR
AGTTTGTGCATCCCTTGCAG+AGG	0.574257	tig0025614:+49672	Msa1424060:CDS
GACATGATTCAGCCTCTGCA+AGG	0.577497	tig0025614:-49684	None:intergenic
CCAATTCTATGGGACAGGAT+AGG	0.591185	tig0025614:-49740	None:intergenic
CTCTCTGTTGTCAAACATGG+TGG	0.591404	tig0025614:+49880	Msa1424060:three_prime_UTR
CCGGATTGTTCTCATGCATG+CGG	0.605542	tig0025614:+49613	Msa1424060:CDS
GTGACTTATGTGCCTTGAAG+AGG	0.613557	tig0025614:-48208	None:intergenic
AGAGGCTGAATCATGTCCAA+TGG	0.629051	tig0025614:+49690	Msa1424060:CDS
CAAAATAGATGAAAGTAAAG+GGG	0.631549	tig0025614:+49510	Msa1424060:CDS
ACATGATTCAGCCTCTGCAA+GGG	0.634476	tig0025614:-49683	None:intergenic
CTGACCATAACCAATCTGCA+TGG	0.667811	tig0025614:-49646	None:intergenic
GAAGATCAAAACATACGCGG+CGG	0.743202	tig0025614:+49557	Msa1424060:CDS
AAGGAAGATCAAAACATACG+CGG	0.753292	tig0025614:+49554	Msa1424060:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATAATAATAATAATAATA+AGG	+	tig0025614:48673-48692	Msa1424060:intron	0.0%
!!	TAATAATAATAATAATAATA+AGG	+	tig0025614:48673-48692	Msa1424060:intron	0.0%
!!	TGACATTAATAAAAAAAAAA+TGG	+	tig0025614:48300-48319	Msa1424060:intron	10.0%
!!!	GATTATTTTTTTCATAAAAA+AGG	+	tig0025614:48972-48991	Msa1424060:intron	10.0%
!!	TGACATTAATAAAAAAAAAA+TGG	+	tig0025614:48300-48319	Msa1424060:intron	10.0%
!!!	GATTATTTTTTTCATAAAAA+AGG	+	tig0025614:48972-48991	Msa1424060:intron	10.0%
!!	TTGGAATATAAAGTAAAATA+AGG	-	tig0025614:48182-48201	None:intergenic	15.0%
!!	TAGAATCTTAAGTAAAAAAT+TGG	-	tig0025614:48252-48271	None:intergenic	15.0%
!!!	ATTGTCATTTTATAACATAA+AGG	-	tig0025614:48844-48863	None:intergenic	15.0%
!!!	GTTGTTATTTTATAACATAA+AGG	+	tig0025614:49003-49022	Msa1424060:intron	15.0%
!!!	TAATCCTTTAACTTAATTTT+AGG	-	tig0025614:49070-49089	None:intergenic	15.0%
!!!	TGTTAATTTTCATGTTAATT+AGG	+	tig0025614:49457-49476	Msa1424060:intron	15.0%
!!!	GTTAATTTTCATGTTAATTA+GGG	+	tig0025614:49458-49477	Msa1424060:intron	15.0%
!!	AAACTAAAAAAAACAAAACA+AGG	-	tig0025614:49833-49852	None:intergenic	15.0%
!!	TTGGAATATAAAGTAAAATA+AGG	-	tig0025614:48182-48201	None:intergenic	15.0%
!!	TAGAATCTTAAGTAAAAAAT+TGG	-	tig0025614:48252-48271	None:intergenic	15.0%
!!!	ATTGTCATTTTATAACATAA+AGG	-	tig0025614:48844-48863	None:intergenic	15.0%
!!!	GTTGTTATTTTATAACATAA+AGG	+	tig0025614:49003-49022	Msa1424060:intron	15.0%
!!!	TAATCCTTTAACTTAATTTT+AGG	-	tig0025614:49070-49089	None:intergenic	15.0%
!!!	TGTTAATTTTCATGTTAATT+AGG	+	tig0025614:49457-49476	Msa1424060:intron	15.0%
!!!	GTTAATTTTCATGTTAATTA+GGG	+	tig0025614:49458-49477	Msa1424060:intron	15.0%
!!	AAACTAAAAAAAACAAAACA+AGG	-	tig0025614:49833-49852	None:intergenic	15.0%
!!	TTAATAGGGAAAATTTATAG+AGG	+	tig0025614:48407-48426	Msa1424060:intron	20.0%
!!	TAATAGGGAAAATTTATAGA+GGG	+	tig0025614:48408-48427	Msa1424060:intron	20.0%
!!	AATCGTGACAAAAAAAATAA+AGG	-	tig0025614:48817-48836	None:intergenic	20.0%
!!!	TCAATGAAATTTGATTTTCT+AGG	+	tig0025614:48890-48909	Msa1424060:intron	20.0%
!!!	TGAGTTTGATGAATTTTTTT+TGG	+	tig0025614:48942-48961	Msa1424060:intron	20.0%
!!	GTTACCTAAAATTAAGTTAA+AGG	+	tig0025614:49063-49082	Msa1424060:intron	20.0%
!!	CTAAAATATGCATACTAAAT+TGG	+	tig0025614:49392-49411	Msa1424060:intron	20.0%
!!	AACAAAATAGATGAAAGTAA+AGG	+	tig0025614:49508-49527	Msa1424060:CDS	20.0%
!!	ACAAAATAGATGAAAGTAAA+GGG	+	tig0025614:49509-49528	Msa1424060:CDS	20.0%
!!	TCTATTATAACCAATTCTAT+GGG	-	tig0025614:49753-49772	None:intergenic	20.0%
!!	TTCTATTATAACCAATTCTA+TGG	-	tig0025614:49754-49773	None:intergenic	20.0%
!!	AAAAACAAAACAAGGAAAAT+GGG	-	tig0025614:49825-49844	None:intergenic	20.0%
!!	AAAAAACAAAACAAGGAAAA+TGG	-	tig0025614:49826-49845	None:intergenic	20.0%
!!	TTAATAGGGAAAATTTATAG+AGG	+	tig0025614:48407-48426	Msa1424060:intron	20.0%
!!	TAATAGGGAAAATTTATAGA+GGG	+	tig0025614:48408-48427	Msa1424060:intron	20.0%
!!	AATCGTGACAAAAAAAATAA+AGG	-	tig0025614:48817-48836	None:intergenic	20.0%
!!!	TCAATGAAATTTGATTTTCT+AGG	+	tig0025614:48890-48909	Msa1424060:intron	20.0%
!!!	TGAGTTTGATGAATTTTTTT+TGG	+	tig0025614:48942-48961	Msa1424060:intron	20.0%
!!	GTTACCTAAAATTAAGTTAA+AGG	+	tig0025614:49063-49082	Msa1424060:intron	20.0%
!!	CTAAAATATGCATACTAAAT+TGG	+	tig0025614:49392-49411	Msa1424060:intron	20.0%
!!	AACAAAATAGATGAAAGTAA+AGG	+	tig0025614:49508-49527	Msa1424060:CDS	20.0%
!!	ACAAAATAGATGAAAGTAAA+GGG	+	tig0025614:49509-49528	Msa1424060:CDS	20.0%
!!	TCTATTATAACCAATTCTAT+GGG	-	tig0025614:49753-49772	None:intergenic	20.0%
!!	TTCTATTATAACCAATTCTA+TGG	-	tig0025614:49754-49773	None:intergenic	20.0%
!!	AAAAACAAAACAAGGAAAAT+GGG	-	tig0025614:49825-49844	None:intergenic	20.0%
!!	AAAAAACAAAACAAGGAAAA+TGG	-	tig0025614:49826-49845	None:intergenic	20.0%
!	AATAGGGAAAATTTATAGAG+GGG	+	tig0025614:48409-48428	Msa1424060:intron	25.0%
!	CAAAATTAGTGGGAAAAAAA+TGG	-	tig0025614:48495-48514	None:intergenic	25.0%
!	CAAGAAAAAGCAAAATTAGT+GGG	-	tig0025614:48505-48524	None:intergenic	25.0%
!	TCAAGAAAAAGCAAAATTAG+TGG	-	tig0025614:48506-48525	None:intergenic	25.0%
!	GTTACCTAAAATTAAGTCAA+GGG	-	tig0025614:48786-48805	None:intergenic	25.0%
!	TGTTACCTAAAATTAAGTCA+AGG	-	tig0025614:48787-48806	None:intergenic	25.0%
!	TCGTGACAAAAAAAAGATAA+AGG	+	tig0025614:49032-49051	Msa1424060:intron	25.0%
!!!	TATTTTAGTGCTTTTTTGAG+TGG	-	tig0025614:49380-49399	None:intergenic	25.0%
!	CAAAATAGATGAAAGTAAAG+GGG	+	tig0025614:49510-49529	Msa1424060:CDS	25.0%
!	AATAGGGAAAATTTATAGAG+GGG	+	tig0025614:48409-48428	Msa1424060:intron	25.0%
!	CAAAATTAGTGGGAAAAAAA+TGG	-	tig0025614:48495-48514	None:intergenic	25.0%
!	CAAGAAAAAGCAAAATTAGT+GGG	-	tig0025614:48505-48524	None:intergenic	25.0%
!	TCAAGAAAAAGCAAAATTAG+TGG	-	tig0025614:48506-48525	None:intergenic	25.0%
!	GTTACCTAAAATTAAGTCAA+GGG	-	tig0025614:48786-48805	None:intergenic	25.0%
!	TGTTACCTAAAATTAAGTCA+AGG	-	tig0025614:48787-48806	None:intergenic	25.0%
!	TCGTGACAAAAAAAAGATAA+AGG	+	tig0025614:49032-49051	Msa1424060:intron	25.0%
!!!	TATTTTAGTGCTTTTTTGAG+TGG	-	tig0025614:49380-49399	None:intergenic	25.0%
!	CAAAATAGATGAAAGTAAAG+GGG	+	tig0025614:49510-49529	Msa1424060:CDS	25.0%
	TGCAATTTCAAAATGTTGAG+TGG	-	tig0025614:48108-48127	None:intergenic	30.0%
	AGAAATTAACCATGATGAAG+AGG	+	tig0025614:48130-48149	Msa1424060:exon	30.0%
	TTTAGTGACAGAATTAGAGA+AGG	-	tig0025614:48446-48465	None:intergenic	30.0%
!	TAGTCCCTTGACTTAATTTT+AGG	+	tig0025614:48779-48798	Msa1424060:intron	30.0%
!	TTTTCATGTTAATTAGGGCA+TGG	+	tig0025614:49463-49482	Msa1424060:intron	30.0%
	AAACAAAATCTGCATTGGAA+AGG	+	tig0025614:49535-49554	Msa1424060:CDS	30.0%
	TCATGCTTCAATTTCTAACT+TGG	+	tig0025614:49781-49800	Msa1424060:three_prime_UTR	30.0%
!	CAAAAACCATTTTCATTGCT+AGG	-	tig0025614:49915-49934	None:intergenic	30.0%
!	CTAGCAATGAAAATGGTTTT+TGG	+	tig0025614:49913-49932	Msa1424060:three_prime_UTR	30.0%
	TGCAATTTCAAAATGTTGAG+TGG	-	tig0025614:48108-48127	None:intergenic	30.0%
	AGAAATTAACCATGATGAAG+AGG	+	tig0025614:48130-48149	Msa1424060:exon	30.0%
	TTTAGTGACAGAATTAGAGA+AGG	-	tig0025614:48446-48465	None:intergenic	30.0%
!	TAGTCCCTTGACTTAATTTT+AGG	+	tig0025614:48779-48798	Msa1424060:intron	30.0%
!	TTTTCATGTTAATTAGGGCA+TGG	+	tig0025614:49463-49482	Msa1424060:intron	30.0%
	AAACAAAATCTGCATTGGAA+AGG	+	tig0025614:49535-49554	Msa1424060:CDS	30.0%
	TCATGCTTCAATTTCTAACT+TGG	+	tig0025614:49781-49800	Msa1424060:three_prime_UTR	30.0%
!	CAAAAACCATTTTCATTGCT+AGG	-	tig0025614:49915-49934	None:intergenic	30.0%
!	CTAGCAATGAAAATGGTTTT+TGG	+	tig0025614:49913-49932	Msa1424060:three_prime_UTR	30.0%
	GAACTAAGAAGAACATCTCA+AGG	+	tig0025614:48074-48093	Msa1424060:five_prime_UTR	35.0%
	AACCATGATGAAGAGGAATT+TGG	+	tig0025614:48137-48156	Msa1424060:exon	35.0%
	AAGGAAGATCAAAACATACG+CGG	+	tig0025614:49554-49573	Msa1424060:CDS	35.0%
	TATAACCAATTCTATGGGAC+AGG	-	tig0025614:49748-49767	None:intergenic	35.0%
	TTTCTCTCTGTTGTCAAACA+TGG	+	tig0025614:49877-49896	Msa1424060:three_prime_UTR	35.0%
	GAACTAAGAAGAACATCTCA+AGG	+	tig0025614:48074-48093	Msa1424060:five_prime_UTR	35.0%
	AACCATGATGAAGAGGAATT+TGG	+	tig0025614:48137-48156	Msa1424060:exon	35.0%
	AAGGAAGATCAAAACATACG+CGG	+	tig0025614:49554-49573	Msa1424060:CDS	35.0%
	TATAACCAATTCTATGGGAC+AGG	-	tig0025614:49748-49767	None:intergenic	35.0%
	TTTCTCTCTGTTGTCAAACA+TGG	+	tig0025614:49877-49896	Msa1424060:three_prime_UTR	35.0%
	GACCAAATTCCTCTTCATCA+TGG	-	tig0025614:48142-48161	None:intergenic	40.0%
	TTCCAATCATCACCTCTTCA+AGG	+	tig0025614:48196-48215	Msa1424060:CDS	40.0%
	GGACATTCAGATCTTGATGT+AGG	+	tig0025614:49484-49503	Msa1424060:CDS	40.0%
	GGGCAAAACAAAATCTGCAT+TGG	+	tig0025614:49530-49549	Msa1424060:CDS	40.0%
	GTTATGGCACATGCATTTGT+AGG	-	tig0025614:49716-49735	None:intergenic	40.0%
!	GTTGTCAAACATGGTGGAAA+CGG	+	tig0025614:49886-49905	Msa1424060:three_prime_UTR	40.0%
	CGGTTTCCTAGCAATGAAAA+TGG	+	tig0025614:49906-49925	Msa1424060:three_prime_UTR	40.0%
	GACCAAATTCCTCTTCATCA+TGG	-	tig0025614:48142-48161	None:intergenic	40.0%
	TTCCAATCATCACCTCTTCA+AGG	+	tig0025614:48196-48215	Msa1424060:CDS	40.0%
	GGACATTCAGATCTTGATGT+AGG	+	tig0025614:49484-49503	Msa1424060:CDS	40.0%
	GGGCAAAACAAAATCTGCAT+TGG	+	tig0025614:49530-49549	Msa1424060:CDS	40.0%
	GTTATGGCACATGCATTTGT+AGG	-	tig0025614:49716-49735	None:intergenic	40.0%
!	GTTGTCAAACATGGTGGAAA+CGG	+	tig0025614:49886-49905	Msa1424060:three_prime_UTR	40.0%
	CGGTTTCCTAGCAATGAAAA+TGG	+	tig0025614:49906-49925	Msa1424060:three_prime_UTR	40.0%
	TGCCTTGAAGAGGTGATGAT+TGG	-	tig0025614:48201-48220	None:intergenic	45.0%
	GTGACTTATGTGCCTTGAAG+AGG	-	tig0025614:48211-48230	None:intergenic	45.0%
	GAAGATCAAAACATACGCGG+CGG	+	tig0025614:49557-49576	Msa1424060:CDS	45.0%
	ATCATGCTCACCATGCAGAT+TGG	+	tig0025614:49636-49655	Msa1424060:CDS	45.0%
!	CTCACCATGCAGATTGGTTA+TGG	+	tig0025614:49642-49661	Msa1424060:CDS	45.0%
	CTGACCATAACCAATCTGCA+TGG	-	tig0025614:49649-49668	None:intergenic	45.0%
	ACATGATTCAGCCTCTGCAA+GGG	-	tig0025614:49686-49705	None:intergenic	45.0%
	AGAGGCTGAATCATGTCCAA+TGG	+	tig0025614:49690-49709	Msa1424060:CDS	45.0%
	CACATGCATTTGTAGGCCAT+TGG	-	tig0025614:49709-49728	None:intergenic	45.0%
!!	GGACAGGATAGGACTTGTTA+TGG	-	tig0025614:49732-49751	None:intergenic	45.0%
	CCAATTCTATGGGACAGGAT+AGG	-	tig0025614:49743-49762	None:intergenic	45.0%
	CCTATCCTGTCCCATAGAAT+TGG	+	tig0025614:49740-49759	Msa1424060:exon	45.0%
	CTCTCTGTTGTCAAACATGG+TGG	+	tig0025614:49880-49899	Msa1424060:three_prime_UTR	45.0%
	TGCCTTGAAGAGGTGATGAT+TGG	-	tig0025614:48201-48220	None:intergenic	45.0%
	GTGACTTATGTGCCTTGAAG+AGG	-	tig0025614:48211-48230	None:intergenic	45.0%
	GAAGATCAAAACATACGCGG+CGG	+	tig0025614:49557-49576	Msa1424060:CDS	45.0%
	ATCATGCTCACCATGCAGAT+TGG	+	tig0025614:49636-49655	Msa1424060:CDS	45.0%
!	CTCACCATGCAGATTGGTTA+TGG	+	tig0025614:49642-49661	Msa1424060:CDS	45.0%
	CTGACCATAACCAATCTGCA+TGG	-	tig0025614:49649-49668	None:intergenic	45.0%
	ACATGATTCAGCCTCTGCAA+GGG	-	tig0025614:49686-49705	None:intergenic	45.0%
	AGAGGCTGAATCATGTCCAA+TGG	+	tig0025614:49690-49709	Msa1424060:CDS	45.0%
	CACATGCATTTGTAGGCCAT+TGG	-	tig0025614:49709-49728	None:intergenic	45.0%
!!	GGACAGGATAGGACTTGTTA+TGG	-	tig0025614:49732-49751	None:intergenic	45.0%
	CCAATTCTATGGGACAGGAT+AGG	-	tig0025614:49743-49762	None:intergenic	45.0%
	CCTATCCTGTCCCATAGAAT+TGG	+	tig0025614:49740-49759	Msa1424060:exon	45.0%
	CTCTCTGTTGTCAAACATGG+TGG	+	tig0025614:49880-49899	Msa1424060:three_prime_UTR	45.0%
!!	TTAAAAATACTTAAATTAAT+AGG	+	tig0025614:48392-48411	Msa1424060:intron	5.0%
!!	TAAAAATACTTAAATTAATA+GGG	+	tig0025614:48393-48412	Msa1424060:intron	5.0%
!!	TTAAAAATACTTAAATTAAT+AGG	+	tig0025614:48392-48411	Msa1424060:intron	5.0%
!!	TAAAAATACTTAAATTAATA+GGG	+	tig0025614:48393-48412	Msa1424060:intron	5.0%
	CCGCATGCATGAGAACAATC+CGG	-	tig0025614:49616-49635	None:intergenic	50.0%
	CCGGATTGTTCTCATGCATG+CGG	+	tig0025614:49613-49632	Msa1424060:CDS	50.0%
	AGTTTGTGCATCCCTTGCAG+AGG	+	tig0025614:49672-49691	Msa1424060:CDS	50.0%
	GACATGATTCAGCCTCTGCA+AGG	-	tig0025614:49687-49706	None:intergenic	50.0%
	CCGCATGCATGAGAACAATC+CGG	-	tig0025614:49616-49635	None:intergenic	50.0%
	CCGGATTGTTCTCATGCATG+CGG	+	tig0025614:49613-49632	Msa1424060:CDS	50.0%
	AGTTTGTGCATCCCTTGCAG+AGG	+	tig0025614:49672-49691	Msa1424060:CDS	50.0%
	GACATGATTCAGCCTCTGCA+AGG	-	tig0025614:49687-49706	None:intergenic	50.0%
	ATCAAAACATACGCGGCGGC+CGG	+	tig0025614:49561-49580	Msa1424060:CDS	55.0%
	CCAGCTACTTGCATCGTGTC+CGG	-	tig0025614:49583-49602	None:intergenic	55.0%
!!	CGATGCAAGTAGCTGGTTCG+AGG	+	tig0025614:49587-49606	Msa1424060:CDS	55.0%
!!	AGTAGCTGGTTCGAGGTTGC+CGG	+	tig0025614:49594-49613	Msa1424060:CDS	55.0%
	ATCAAAACATACGCGGCGGC+CGG	+	tig0025614:49561-49580	Msa1424060:CDS	55.0%
	CCAGCTACTTGCATCGTGTC+CGG	-	tig0025614:49583-49602	None:intergenic	55.0%
!!	CGATGCAAGTAGCTGGTTCG+AGG	+	tig0025614:49587-49606	Msa1424060:CDS	55.0%
!!	AGTAGCTGGTTCGAGGTTGC+CGG	+	tig0025614:49594-49613	Msa1424060:CDS	55.0%
	CCGGACACGATGCAAGTAGC+TGG	+	tig0025614:49580-49599	Msa1424060:CDS	60.0%
	CCGGACACGATGCAAGTAGC+TGG	+	tig0025614:49580-49599	Msa1424060:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0025614	gene	48070	50002	48070	ID=Msa1424060;Name=Msa1424060
tig0025614	mRNA	48070	50002	48070	ID=Msa1424060-mRNA-1;Parent=Msa1424060;Name=Msa1424060-mRNA-1;_AED=0.00;_eAED=0.00;_QI=71\|1\|1\|1\|0\|0\|2\|246\|125
tig0025614	exon	48070	48240	48070	ID=Msa1424060-mRNA-1:exon:3568;Parent=Msa1424060-mRNA-1
tig0025614	exon	49479	50002	49479	ID=Msa1424060-mRNA-1:exon:3569;Parent=Msa1424060-mRNA-1
tig0025614	five_prime_UTR	48070	48140	48070	ID=Msa1424060-mRNA-1:five_prime_utr;Parent=Msa1424060-mRNA-1
tig0025614	CDS	48141	48240	48141	ID=Msa1424060-mRNA-1:cds;Parent=Msa1424060-mRNA-1
tig0025614	CDS	49479	49756	49479	ID=Msa1424060-mRNA-1:cds;Parent=Msa1424060-mRNA-1
tig0025614	three_prime_UTR	49757	50002	49757	ID=Msa1424060-mRNA-1:three_prime_utr;Parent=Msa1424060-mRNA-1

Gene Sequence	>Msa1424060 ATGATGAAGAGGAATTTGGTCTATGCTGCTGCATTTGTCCTTATTTTACTTTATATTCCAATCATCACCTCTTCAAGGCACATAAGTCACTCAAATTCGCGGCATGGACATTCAGATCTTGATGTAGGAAACAAAATAGATGAAAGTAAAGGGGCAAAACAAAATCTGCATTGGAAAGGAAGATCAAAACATACGCGGCGGCCGGACACGATGCAAGTAGCTGGTTCGAGGTTGCCGGATTGTTCTCATGCATGCGGATCATGCTCACCATGCAGATTGGTTATGGTCAGCTTAGTTTGTGCATCCCTTGCAGAGGCTGAATCATGTCCAATGGCCTACAAATGCATGTGCCATAACAAGTCCTATCCTGTCCCATAG
Protein sequence	>Msa1424060 MMKRNLVYAAAFVLILLYIPIITSSRHISHSNSRHGHSDLDVGNKIDESKGAKQNLHWKGRSKHTRRPDTMQVAGSRLPDCSHACGSCSPCRLVMVSLVCASLAEAESCPMAYKCMCHNKSYPVP*