AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1312730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1312730 MtrunA17_Chr8g0341001 83.099 355 3 2 1 298 1 355 0.0 574
Msa1312730 MtrunA17_Chr2g0330781 65.242 351 65 2 4 297 3 353 1.08e-159 449
Msa1312730 MtrunA17_Chr2g0320671 49.577 355 119 4 1 298 1 352 3.70e-112 328
Msa1312730 MtrunA17_Chr4g0027521 41.945 329 132 4 29 298 24 352 3.48e-81 249
Msa1312730 MtrunA17_Chr5g0438621 37.353 340 148 5 10 290 11 344 5.57e-71 223
Msa1312730 MtrunA17_Chr3g0105401 36.628 344 155 5 7 290 3 343 1.61e-68 216
Msa1312730 MtrunA17_Chr5g0438631 34.604 341 157 6 10 290 9 343 2.74e-68 216
Msa1312730 MtrunA17_Chr3g0120861 37.152 323 145 4 30 295 36 357 1.41e-67 214
Msa1312730 MtrunA17_Chr5g0438641 35.535 318 147 3 30 290 30 346 3.65e-67 213
Msa1312730 MtrunA17_Chr1g0156931 36.873 339 154 6 10 290 17 353 3.64e-65 208
Msa1312730 MtrunA17_Chr5g0432101 37.778 315 134 6 32 286 33 345 8.46e-64 205
Msa1312730 MtrunA17_Chr2g0283191 32.845 341 160 7 8 281 14 352 1.01e-60 197
Msa1312730 MtrunA17_Chr8g0371471 34.700 317 149 4 31 290 40 355 7.02e-60 194
Msa1312730 MtrunA17_Chr8g0371491 35.669 314 144 4 30 286 34 346 1.48e-59 194
Msa1312730 MtrunA17_Chr8g0371481 34.591 318 149 5 31 290 40 356 6.85e-58 189
Msa1312730 MtrunA17_Chr5g0405821 35.238 315 144 5 30 285 41 354 7.68e-56 184
Msa1312730 MtrunA17_Chr1g0156921 33.634 333 155 6 10 280 18 346 1.54e-54 181
Msa1312730 MtrunA17_Chr7g0229591 46.970 198 99 2 1 196 1 194 1.77e-54 176
Msa1312730 MtrunA17_Chr3g0142941 35.350 314 144 6 30 286 45 356 2.15e-53 178
Msa1312730 MtrunA17_Chr5g0405831 32.622 328 159 6 19 285 27 353 6.17e-53 177
Msa1312730 MtrunA17_Chr1g0156891 32.479 351 170 8 4 290 10 357 4.97e-52 174
Msa1312730 MtrunA17_Chr3g0142921 32.849 344 166 6 10 290 9 350 1.40e-51 173
Msa1312730 MtrunA17_Chr1g0156911 33.546 313 149 6 31 286 36 346 1.79e-51 173
Msa1312730 MtrunA17_Chr1g0156901 34.069 317 150 5 27 286 45 359 3.15e-51 172
Msa1312730 MtrunA17_Chr4g0046151 43.850 187 104 1 10 196 15 200 7.45e-51 171
Msa1312730 MtrunA17_Chr7g0275021 29.915 351 179 10 4 290 1 348 2.87e-45 157
Msa1312730 MtrunA17_Chr7g0274991 30.233 344 173 9 12 291 8 348 8.24e-45 155
Msa1312730 MtrunA17_Chr2g0285061 32.448 339 158 12 10 286 13 342 1.80e-41 147
Msa1312730 MtrunA17_Chr1g0189891 29.706 340 182 7 12 297 9 345 8.91e-41 145
Msa1312730 MtrunA17_Chr1g0189901 30.721 319 159 8 32 291 31 346 2.15e-40 144
Msa1312730 MtrunA17_Chr6g0457921 31.250 352 172 13 1 290 1 344 4.08e-40 143
Msa1312730 MtrunA17_Chr1g0182811 29.143 350 182 8 4 289 1 348 5.28e-40 143
Msa1312730 MtrunA17_Chr5g0437421 29.503 322 154 7 31 285 31 346 5.78e-40 142
Msa1312730 MtrunA17_Chr8g0380041 32.840 338 159 12 10 286 10 340 1.02e-39 142
Msa1312730 MtrunA17_Chr1g0157001 29.703 303 155 5 37 283 41 341 1.78e-38 139
Msa1312730 MtrunA17_Chr5g0396511 28.344 314 163 7 35 289 49 359 2.13e-38 139
Msa1312730 MtrunA17_Chr4g0027571 36.364 220 82 3 135 297 3 221 2.26e-38 135
Msa1312730 MtrunA17_Chr8g0374101 28.814 354 187 8 1 290 6 358 3.82e-38 138
Msa1312730 MtrunA17_Chr3g0086641 30.769 338 165 11 11 286 17 347 3.98e-38 138
Msa1312730 MtrunA17_Chr4g0008851 27.920 351 189 8 1 290 1 348 3.98e-38 138
Msa1312730 MtrunA17_Chr7g0275011 29.855 345 176 11 4 290 12 348 5.49e-38 137
Msa1312730 MtrunA17_Chr3g0099161 31.378 341 167 13 12 289 71 407 9.55e-38 138
Msa1312730 MtrunA17_Chr7g0275001 28.242 347 182 9 4 290 1 340 1.28e-37 136
Msa1312730 MtrunA17_Chr3g0077571 31.231 333 164 12 11 286 11 335 4.91e-37 135
Msa1312730 MtrunA17_Chr6g0469511 53.846 117 54 0 27 143 25 141 6.89e-36 125
Msa1312730 MtrunA17_Chr4g0060811 30.892 314 155 10 36 290 31 341 7.70e-36 132
Msa1312730 MtrunA17_Chr3g0099151 29.532 342 173 13 12 289 66 403 1.05e-35 132
Msa1312730 MtrunA17_Chr3g0092131 30.499 341 169 12 9 290 6 337 1.54e-35 131
Msa1312730 MtrunA17_Chr7g0215961 26.686 341 173 9 11 290 10 334 3.25e-35 130
Msa1312730 MtrunA17_Chr2g0284941 31.579 323 171 12 7 289 6 318 4.27e-35 130
Msa1312730 MtrunA17_Chr2g0294301 28.882 322 164 8 30 290 33 350 2.47e-34 128
Msa1312730 MtrunA17_Chr2g0284951 30.573 314 149 10 30 286 31 332 7.87e-34 127
Msa1312730 MtrunA17_Chr1g0189931 27.381 336 186 7 12 290 12 346 8.72e-34 126
Msa1312730 MtrunA17_Chr4g0027561 50.000 126 57 2 1 124 1 122 6.38e-33 118
Msa1312730 MtrunA17_Chr3g0099231 29.118 340 174 13 12 289 8 342 6.49e-33 124
Msa1312730 MtrunA17_Chr5g0434621 30.000 340 167 13 12 289 6 336 7.37e-33 124
Msa1312730 MtrunA17_Chr2g0284921 28.963 328 164 9 18 286 20 337 9.52e-33 124
Msa1312730 MtrunA17_Chr4g0017431 27.011 348 185 11 1 286 1 341 1.41e-32 123
Msa1312730 MtrunA17_Chr4g0068441 27.077 325 172 8 30 293 33 353 3.94e-32 122
Msa1312730 MtrunA17_Chr7g0229581 46.400 125 43 2 196 297 31 154 2.06e-30 112
Msa1312730 MtrunA17_Chr2g0284991 30.435 345 167 13 7 289 6 339 2.60e-30 117
Msa1312730 MtrunA17_Chr5g0420811 30.696 316 156 12 30 291 31 337 1.11e-29 115
Msa1312730 MtrunA17_Chr3g0077581 28.660 321 160 10 30 286 34 349 2.32e-29 114
Msa1312730 MtrunA17_Chr2g0301641 28.182 330 167 10 32 297 43 366 3.77e-29 114
Msa1312730 MtrunA17_Chr3g0085881 29.936 314 153 11 30 288 31 332 7.69e-29 113
Msa1312730 MtrunA17_Chr2g0285001 28.994 338 167 13 13 286 9 337 5.49e-28 111
Msa1312730 MtrunA17_Chr8g0380031 29.085 306 166 9 30 287 82 384 4.58e-27 109
Msa1312730 MtrunA17_Chr5g0444401 32.549 255 130 8 4 243 1 228 6.27e-27 108
Msa1312730 MtrunA17_Chr4g0018571 44.355 124 45 2 197 297 55 177 6.43e-26 101
Msa1312730 MtrunA17_Chr8g0370951 27.168 346 184 14 9 289 7 349 7.34e-26 105
Msa1312730 MtrunA17_Chr2g0294311 25.915 355 180 12 10 290 10 355 2.09e-25 103
Msa1312730 MtrunA17_Chr8g0349341 28.481 316 156 13 32 286 37 343 4.58e-25 103
Msa1312730 MtrunA17_Chr8g0344931 26.970 330 162 11 32 298 37 350 1.98e-24 101
Msa1312730 MtrunA17_Chr3g0085231 28.659 328 181 12 9 288 9 331 7.70e-24 99.4
Msa1312730 MtrunA17_Chr8g0370981 25.128 390 190 12 10 297 19 408 1.42e-23 99.8
Msa1312730 MtrunA17_Chr8g0349321 27.129 317 159 13 32 286 37 343 2.64e-23 98.2
Msa1312730 MtrunA17_Chr3g0131321 37.500 152 91 3 29 177 31 181 5.82e-23 97.4
Msa1312730 MtrunA17_Chr8g0349331 29.110 292 155 11 32 286 35 311 6.96e-23 96.7
Msa1312730 MtrunA17_Chr8g0344941 26.168 321 168 10 32 289 41 355 7.29e-22 94.4
Msa1312730 MtrunA17_Chr1g0189921 39.844 128 67 2 12 130 11 137 6.24e-21 87.0
Msa1312730 MtrunA17_Chr4g0027581 34.965 143 47 2 196 293 49 190 6.68e-20 85.9
Msa1312730 MtrunA17_Chr2g0321991 50.000 90 43 2 30 118 31 119 1.96e-19 82.4
Msa1312730 MtrunA17_Chr1g0160031 27.147 361 173 15 9 290 17 366 2.48e-19 87.0
Msa1312730 MtrunA17_Chr6g0455661 44.915 118 57 5 4 118 1 113 3.14e-18 84.7
Msa1312730 MtrunA17_Chr8g0349301 36.735 147 79 5 32 173 37 174 1.99e-17 81.3
Msa1312730 MtrunA17_Chr1g0159991 25.676 370 182 15 1 289 7 364 3.57e-17 80.9
Msa1312730 MtrunA17_Chr4g0018561 57.895 57 24 0 26 82 24 80 4.14e-17 75.5
Msa1312730 MtrunA17_Chr2g0283481 26.171 363 173 16 8 290 26 373 4.59e-17 80.9
Msa1312730 MtrunA17_Chr6g0469521 41.414 99 34 2 196 271 55 152 2.19e-16 74.7
Msa1312730 MtrunA17_Chr1g0159981 24.793 363 186 14 7 289 19 374 5.77e-16 77.4
Msa1312730 MtrunA17_Chr2g0283531 28.060 335 157 16 32 290 31 357 1.56e-15 76.3
Msa1312730 MtrunA17_Chr2g0301581 27.920 351 185 16 1 290 1 344 1.59e-15 75.9
Msa1312730 MtrunA17_Chr2g0301541 26.781 351 189 15 1 290 1 344 2.39e-15 75.5
Msa1312730 MtrunA17_Chr8g0381581 23.200 375 186 14 7 290 9 372 4.26e-15 75.1
Msa1312730 MtrunA17_Chr6g0463261 38.144 97 60 0 82 178 1 97 5.56e-15 70.5
Msa1312730 MtrunA17_Chr7g0273101 25.444 338 168 14 31 290 28 359 9.93e-15 73.9
Msa1312730 MtrunA17_Chr5g0432081 57.143 56 22 2 234 289 10 63 7.27e-14 65.9
Msa1312730 MtrunA17_Chr8g0370971 41.304 92 50 1 31 118 37 128 1.13e-13 67.8
Msa1312730 MtrunA17_Chr2g0308201 44.737 76 41 1 10 85 13 87 4.77e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1312730 AT3G16370.1 62.952 332 64 3 23 297 23 352 3.80e-142 404
Msa1312730 AT5G22810.1 49.091 330 107 4 29 298 31 359 3.32e-105 311
Msa1312730 AT5G03820.1 46.240 359 127 6 4 298 1 357 5.29e-102 303
Msa1312730 AT5G03820.2 47.674 344 119 5 14 298 44 385 8.51e-101 301
Msa1312730 AT5G03810.1 46.705 349 126 5 8 298 7 353 3.01e-100 298
Msa1312730 AT3G53100.1 42.655 354 142 5 1 297 1 350 4.05e-91 275
Msa1312730 AT2G04570.1 36.311 347 159 6 1 290 1 342 1.73e-69 219
Msa1312730 AT2G42990.1 36.909 317 142 6 30 289 26 341 6.12e-67 213
Msa1312730 AT5G42170.1 37.143 315 143 3 30 290 48 361 3.53e-66 211
Msa1312730 AT3G14820.1 36.760 321 139 6 28 290 28 342 2.98e-64 206
Msa1312730 AT5G45960.1 38.462 312 128 7 30 279 45 354 3.32e-63 204
Msa1312730 AT1G20120.1 35.714 322 149 3 26 290 72 392 3.03e-62 202
Msa1312730 AT1G75880.2 36.646 322 146 4 26 290 46 366 6.15e-61 198
Msa1312730 AT4G26790.1 33.841 328 153 5 20 290 23 343 1.55e-60 196
Msa1312730 AT4G26790.2 33.841 328 153 5 20 290 23 343 1.55e-60 196
Msa1312730 AT1G59406.1 34.808 339 164 6 8 290 4 341 1.61e-59 194
Msa1312730 AT1G59030.1 34.808 339 164 6 8 290 4 341 1.61e-59 194
Msa1312730 AT1G58725.1 34.808 339 164 6 8 290 4 341 1.61e-59 194
Msa1312730 AT1G75890.2 35.987 314 142 4 31 286 46 358 2.37e-59 194
Msa1312730 AT1G75880.1 36.533 323 146 5 26 290 46 367 3.82e-59 193
Msa1312730 AT1G75890.3 35.987 314 142 4 31 286 75 387 5.51e-59 194
Msa1312730 AT2G30310.1 33.618 351 150 8 4 281 1 341 5.52e-59 192
Msa1312730 AT1G06990.1 31.988 322 158 4 28 290 33 352 7.82e-59 192
Msa1312730 AT1G58520.3 35.783 313 143 6 30 290 28 334 6.87e-58 189
Msa1312730 AT1G58525.1 35.783 313 143 6 30 290 28 334 6.87e-58 189
Msa1312730 AT3G43570.1 35.577 312 163 6 9 290 9 312 1.47e-57 188
Msa1312730 AT2G30220.1 34.160 363 156 10 4 294 1 352 1.12e-56 187
Msa1312730 AT1G75900.1 33.728 338 165 5 10 290 21 356 1.40e-56 186
Msa1312730 AT1G75890.1 34.557 327 142 5 31 286 46 371 1.13e-55 184
Msa1312730 AT5G45950.1 33.333 315 152 6 30 290 38 348 1.74e-55 183
Msa1312730 AT2G40250.1 33.438 320 147 6 30 286 34 350 6.58e-55 182
Msa1312730 AT2G31540.1 32.665 349 161 9 9 289 8 350 1.05e-54 181
Msa1312730 AT5G63170.1 35.473 296 147 5 30 281 26 321 7.36e-53 176
Msa1312730 AT1G58430.1 34.295 312 143 8 29 280 32 341 1.03e-52 176
Msa1312730 AT2G24560.1 32.836 335 160 7 9 280 8 340 1.67e-51 173
Msa1312730 AT1G20132.1 32.911 316 154 5 31 289 60 374 3.05e-48 165
Msa1312730 AT1G20130.2 30.699 329 163 6 26 290 178 505 5.89e-44 157
Msa1312730 AT1G20130.1 30.699 329 163 6 26 290 198 525 7.23e-44 157
Msa1312730 AT3G04290.1 29.480 346 182 8 4 290 1 343 3.58e-43 152
Msa1312730 AT1G75920.2 30.347 346 171 8 4 290 1 335 2.77e-41 146
Msa1312730 AT1G23500.1 33.131 329 147 6 24 294 27 340 4.64e-41 145
Msa1312730 AT5G18430.1 28.527 319 166 6 32 290 28 344 2.73e-40 144
Msa1312730 AT4G10950.2 29.283 321 163 9 29 289 68 384 2.82e-40 145
Msa1312730 AT3G43550.1 32.443 262 135 4 10 234 8 264 2.99e-40 142
Msa1312730 AT2G24560.2 42.373 177 98 2 9 181 8 184 3.14e-40 142
Msa1312730 AT1G29670.1 32.047 337 162 11 11 286 10 340 3.30e-40 144
Msa1312730 AT1G29670.2 32.047 337 162 11 11 286 78 408 7.93e-40 144
Msa1312730 AT1G20135.1 30.462 325 179 7 1 281 1 322 5.18e-39 140
Msa1312730 AT1G71691.2 33.333 318 148 12 27 286 50 361 7.46e-39 140
Msa1312730 AT1G73610.1 29.560 318 159 7 27 290 30 336 9.42e-39 139
Msa1312730 AT2G23540.1 30.211 331 166 8 32 298 48 377 3.16e-38 139
Msa1312730 AT5G33370.1 31.361 338 161 12 17 290 14 344 4.82e-38 138
Msa1312730 AT4G10950.1 28.972 321 160 10 29 289 68 380 1.50e-37 137
Msa1312730 AT4G28780.1 28.161 348 183 11 9 294 7 349 3.84e-37 135
Msa1312730 AT5G15720.1 31.481 324 152 10 29 288 27 344 1.51e-36 134
Msa1312730 AT1G75910.1 28.818 347 175 7 4 290 1 335 1.52e-36 134
Msa1312730 AT1G29660.1 31.761 318 150 11 30 286 30 341 2.47e-36 134
Msa1312730 AT5G08460.1 27.954 347 179 10 8 286 20 363 3.64e-36 134
Msa1312730 AT1G75920.1 29.014 355 167 9 10 290 2 345 4.75e-36 132
Msa1312730 AT5G41890.1 28.977 352 171 11 9 290 15 357 1.08e-34 129
Msa1312730 AT4G18970.1 30.530 321 157 10 27 286 23 338 3.93e-34 127
Msa1312730 AT5G45670.1 29.240 342 175 11 6 285 2 338 4.58e-34 127
Msa1312730 AT4G18970.2 30.530 321 157 10 27 286 23 338 6.76e-34 128
Msa1312730 AT2G19010.2 28.280 343 177 11 1 286 28 358 1.55e-32 124
Msa1312730 AT5G37690.1 28.060 335 177 10 13 289 10 338 2.31e-32 123
Msa1312730 AT2G19010.1 28.485 330 168 10 13 286 11 328 2.96e-32 122
Msa1312730 AT1G71250.1 30.408 319 154 11 30 286 39 351 3.93e-32 122
Msa1312730 AT2G19060.1 30.599 317 148 13 29 286 27 330 5.22e-32 122
Msa1312730 AT5G33370.2 40.116 172 92 4 17 182 14 180 2.10e-31 120
Msa1312730 AT1G74460.1 29.299 314 160 10 36 290 27 337 2.51e-31 120
Msa1312730 AT1G33811.1 29.969 327 158 12 26 286 28 349 1.09e-30 119
Msa1312730 AT2G19050.1 29.073 313 154 12 30 286 29 329 1.31e-29 115
Msa1312730 AT1G75930.1 25.862 348 184 7 4 290 1 335 3.70e-29 114
Msa1312730 AT4G16230.1 39.735 151 88 2 30 179 28 176 6.43e-29 111
Msa1312730 AT5G37690.2 27.761 335 170 11 13 289 10 330 1.36e-28 112
Msa1312730 AT1G71691.3 34.711 242 119 9 27 233 50 287 2.65e-28 111
Msa1312730 AT1G75920.3 26.879 346 152 9 4 290 1 304 3.06e-28 111
Msa1312730 AT5G55050.1 28.308 325 166 12 30 290 37 358 7.86e-28 111
Msa1312730 AT3G50400.1 38.889 180 104 5 9 183 10 188 2.83e-27 109
Msa1312730 AT5G15720.2 32.794 247 121 7 29 234 27 269 1.00e-26 107
Msa1312730 AT1G75920.4 28.136 295 142 8 55 290 24 307 3.48e-26 105
Msa1312730 AT1G53920.1 28.249 354 179 13 9 293 17 364 1.24e-25 105
Msa1312730 AT1G53940.2 26.703 367 191 12 5 298 3 364 2.97e-25 103
Msa1312730 AT4G30140.1 28.115 313 155 12 31 287 32 330 9.70e-25 102
Msa1312730 AT5G40990.1 27.190 331 172 10 32 298 37 362 6.91e-24 100
Msa1312730 AT1G71120.1 28.037 321 160 12 30 289 29 339 7.47e-24 100
Msa1312730 AT2G19050.2 28.571 266 144 10 8 238 7 261 3.36e-22 94.0
Msa1312730 AT3G14225.1 25.526 333 179 10 32 298 37 366 1.44e-20 90.9
Msa1312730 AT3G14225.2 25.982 331 176 11 32 298 37 362 4.46e-20 89.7
Msa1312730 AT1G53940.1 32.195 205 123 7 5 197 3 203 8.01e-20 88.2
Msa1312730 AT4G16220.1 43.697 119 61 4 1 118 1 114 7.78e-19 84.3
Msa1312730 AT2G31550.1 25.701 214 90 6 139 289 2 209 9.93e-19 83.2
Msa1312730 AT2G03980.2 25.962 312 173 11 31 286 42 351 1.21e-18 85.5
Msa1312730 AT2G03980.3 25.962 312 173 11 31 286 42 351 1.21e-18 85.5
Msa1312730 AT2G03980.1 25.962 312 173 11 31 286 42 351 1.21e-18 85.5
Msa1312730 AT2G03980.4 25.962 312 173 11 31 286 42 351 1.21e-18 85.5
Msa1312730 AT1G28610.2 27.500 360 167 17 9 290 14 357 1.72e-18 85.1
Msa1312730 AT2G04020.1 41.593 113 63 3 31 141 42 153 2.73e-18 82.8
Msa1312730 AT2G04020.2 41.593 113 63 3 31 141 42 153 5.59e-18 83.2
Msa1312730 AT1G53990.1 24.425 348 182 12 10 287 7 343 8.64e-18 83.2
Msa1312730 AT1G28650.1 26.197 355 183 15 10 290 15 364 1.83e-17 82.4
Msa1312730 AT1G28580.1 25.978 358 176 15 9 290 20 364 3.03e-17 81.6
Msa1312730 AT1G71691.1 28.287 251 118 10 94 286 14 260 9.56e-17 79.0
Msa1312730 AT1G28570.1 26.027 365 181 15 9 290 3 361 1.57e-16 79.7
Msa1312730 AT1G67830.1 25.000 348 169 14 25 289 23 361 3.49e-16 78.6
Msa1312730 AT1G28600.1 25.000 360 176 17 9 290 14 357 9.26e-16 77.4
Msa1312730 AT1G28670.1 25.000 356 187 12 10 290 13 363 1.40e-15 76.6
Msa1312730 AT2G27360.1 23.967 363 190 12 9 296 16 367 3.65e-15 75.5
Msa1312730 AT1G28600.2 27.679 224 127 12 9 213 14 221 1.26e-12 66.2
Msa1312730 AT1G31550.2 36.552 145 67 7 9 141 19 150 2.31e-12 67.4
Msa1312730 AT1G31550.1 36.552 145 67 7 9 141 19 150 2.31e-12 67.4
Msa1312730 AT1G28610.1 32.927 164 85 9 32 180 31 184 9.72e-12 64.3
Msa1312730 AT3G14220.1 28.824 170 100 6 31 196 31 183 3.75e-11 63.5

Find 60 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAAGTTCCTAGTGATTT+TGG 0.126371 8_4:-9963026 None:intergenic
CCTGCTGCAAGGACTTTATT+TGG 0.195553 8_4:+9962540 Msa1312730:intron
TATTCTAACTGTTGGGATAA+TGG 0.256406 8_4:-9962108 None:intergenic
GGCAAGTTGGCTCAGGTTGC+TGG 0.317149 8_4:+9962147 Msa1312730:CDS
ATGCAACTCAGTATGTGTTT+TGG 0.323360 8_4:+9963051 Msa1312730:CDS
GCTGATAACTTAATTATTGC+CGG 0.335224 8_4:+9963113 Msa1312730:CDS
CAGTGTATGCTCAGTATATA+TGG 0.344117 8_4:-9960589 None:intergenic
AACTTTGCTTCAGCTGCATC+TGG 0.352556 8_4:+9961459 Msa1312730:CDS
TCAGGTTGCTGGCAGTAAAA+AGG 0.357625 8_4:+9962158 Msa1312730:CDS
AAGGCTAATTACCCTCCTTA+TGG 0.370041 8_4:+9960801 Msa1312730:CDS
TCAGGGAAGAACCTCCTTCT+TGG 0.388520 8_4:+9961432 Msa1312730:CDS
AATCTTCAAAAGCAACTTCC+TGG 0.397330 8_4:+9962642 Msa1312730:CDS
TATCTTAGTCCACAGGCATC+AGG 0.401048 8_4:+9961414 Msa1312730:CDS
CCTTGAAATATTCTAACTGT+TGG 0.403529 8_4:-9962116 None:intergenic
GTACCAGCTATCATAACATT+TGG 0.405659 8_4:+9960729 Msa1312730:CDS
CTTGAAATATTCTAACTGTT+GGG 0.411275 8_4:-9962115 None:intergenic
GGGAAAGGGTGCTGTGGTAC+AGG 0.417817 8_4:+9962972 Msa1312730:CDS
GTGCAAAGCTACCACAAGTT+AGG 0.425459 8_4:-9960695 None:intergenic
TTAGTAAAATCTCTACCATA+AGG 0.435286 8_4:-9960816 None:intergenic
CCAACAGTTAGAATATTTCA+AGG 0.438731 8_4:+9962116 Msa1312730:CDS
CCATGATTGCATTTCATATG+TGG 0.438969 8_4:-9963395 None:intergenic
AAAATTATTATACCAATCCT+TGG 0.441731 8_4:+9962241 Msa1312730:CDS
CCGAAAATGACACGTATTGA+AGG 0.454561 8_4:-9960561 None:intergenic
TTTACTAATAAACAACCTAC+TGG 0.460660 8_4:+9960831 Msa1312730:CDS
TTATTTGGTTACCATGAGAA+TGG 0.467168 8_4:+9962555 Msa1312730:CDS
TCTTTGTATGTATTGAGTGC+TGG 0.485192 8_4:+9962201 Msa1312730:CDS
GGCATAGCTCTTATCACATA+AGG 0.485525 8_4:+9963134 Msa1312730:CDS
TGCATCTGGTTATGATGAAA+AGG 0.486563 8_4:+9961473 Msa1312730:CDS
GAATATTTCAAGGAATATCA+AGG 0.486768 8_4:+9962126 Msa1312730:CDS
CCACATATGAAATGCAATCA+TGG 0.498015 8_4:+9963395 Msa1312730:three_prime_UTR
TCACCAAATGTTATGATAGC+TGG 0.498295 8_4:-9960732 None:intergenic
AAAGGCTGCATCTATTATCA+AGG 0.521328 8_4:+9962176 Msa1312730:CDS
CAAGGAATATCAAGGCAAGT+TGG 0.527876 8_4:+9962134 Msa1312730:CDS
ATATCAAGGCAAGTTGGCTC+AGG 0.531855 8_4:+9962140 Msa1312730:CDS
TTATGTGATAAGAGCTATGC+CGG 0.533000 8_4:-9963132 None:intergenic
GGTGACTCTGCAGTAGATGT+AGG 0.538565 8_4:+9960750 Msa1312730:CDS
GCCTGTGGACTAAGATATGC+AGG 0.541888 8_4:-9961408 None:intergenic
TGCTTGGTTGATCCAAGGAT+TGG 0.542337 8_4:-9962253 None:intergenic
ACTGATCAACAGTGATTGCT+TGG 0.542659 8_4:-9962269 None:intergenic
TTGCAATGGCAAGTTAGCTA+CGG 0.548669 8_4:+9960860 Msa1312730:CDS
GTGATTGCTTGGTTGATCCA+AGG 0.549428 8_4:-9962258 None:intergenic
GTATCTTGCTTCCTAACTTG+TGG 0.552323 8_4:+9960684 Msa1312730:CDS
AGGTTCTTCCCTGATGCCTG+TGG 0.556382 8_4:-9961423 None:intergenic
AAGCAAAGTTAGCACCAAGA+AGG 0.572551 8_4:-9961446 None:intergenic
ACCTGCATATCTTAGTCCAC+AGG 0.573584 8_4:+9961407 Msa1312730:CDS
ACTTGATTAGCAGCTTCTGA+AGG 0.575330 8_4:-9963086 None:intergenic
TTGCGACACAACCATTCTCA+TGG 0.578404 8_4:-9962566 None:intergenic
GTAAAATCTCTACCATAAGG+AGG 0.579599 8_4:-9960813 None:intergenic
ATCTTAGTCCACAGGCATCA+GGG 0.598385 8_4:+9961415 Msa1312730:CDS
TTGTGCAATCCAAAATCACT+AGG 0.603517 8_4:+9963017 Msa1312730:CDS
CAAAGTTAGCACCAAGAAGG+AGG 0.606423 8_4:-9961443 None:intergenic
TAAAATCTCTACCATAAGGA+GGG 0.614379 8_4:-9960812 None:intergenic
CCATTGCAAAACCTCCCAGT+AGG 0.621346 8_4:-9960846 None:intergenic
TTACTAATAAACAACCTACT+GGG 0.623210 8_4:+9960832 Msa1312730:CDS
AGAATCAACACTGATGCACA+AGG 0.629785 8_4:+9962588 Msa1312730:CDS
CTAATAAACAACCTACTGGG+AGG 0.651293 8_4:+9960835 Msa1312730:CDS
GAGGCAGGGAAAGGGTGCTG+TGG 0.662542 8_4:+9962966 Msa1312730:CDS
AAAATGACACGTATTGAAGG+AGG 0.671205 8_4:-9960558 None:intergenic
CACGTATTGAAGGAGGAAGG+TGG 0.679092 8_4:-9960551 None:intergenic
TGACACGTATTGAAGGAGGA+AGG 0.690350 8_4:-9960554 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTAGTTTTTTTCAATTA+AGG + chr8_4:9961712-9961731 Msa1312730:intron 10.0%
!! GTTTACAATTAGTTATTAAA+TGG + chr8_4:9961948-9961967 Msa1312730:intron 15.0%
!! AGTTCAAACATAATATATAT+AGG - chr8_4:9962016-9962035 None:intergenic 15.0%
!! AAAAATTTGATATACTACTT+AGG - chr8_4:9962389-9962408 None:intergenic 15.0%
!! GTATTGTATCCTTTATTATA+TGG + chr8_4:9960934-9960953 Msa1312730:intron 20.0%
!!! ACTAGTTTATAAGTATGAAA+TGG + chr8_4:9961664-9961683 Msa1312730:intron 20.0%
!! ACTATTTGAATTAAGCTATA+AGG + chr8_4:9961775-9961794 Msa1312730:intron 20.0%
!!! TTTTGTTATGAACTTGTTTT+TGG + chr8_4:9962059-9962078 Msa1312730:intron 20.0%
!! AAAATTATTATACCAATCCT+TGG + chr8_4:9962241-9962260 Msa1312730:CDS 20.0%
!! ACATATTTCAGTTTATCTAA+TGG - chr8_4:9962415-9962434 None:intergenic 20.0%
!! TGCAATAACAAAATCAATTT+AGG - chr8_4:9962776-9962795 None:intergenic 20.0%
!! CTATATAGAACATCATAAAA+AGG - chr8_4:9962938-9962957 None:intergenic 20.0%
!!! CTTTTTATGATGTTCTATAT+AGG + chr8_4:9962936-9962955 Msa1312730:intron 20.0%
!!! AAAATGTACTGTACTATTTT+TGG + chr8_4:9963355-9963374 Msa1312730:three_prime_UTR 20.0%
!!! TAATTAGCCTTAAAAAGAGT+AGG - chr8_4:9960792-9960811 None:intergenic 25.0%
! TTAGTAAAATCTCTACCATA+AGG - chr8_4:9960819-9960838 None:intergenic 25.0%
! TTTACTAATAAACAACCTAC+TGG + chr8_4:9960831-9960850 Msa1312730:CDS 25.0%
! TTACTAATAAACAACCTACT+GGG + chr8_4:9960832-9960851 Msa1312730:CDS 25.0%
! TTCAAATAAATCTTAGTCGT+TGG - chr8_4:9961042-9961061 None:intergenic 25.0%
! AACGACTAAGATTTATTTGA+AGG + chr8_4:9961041-9961060 Msa1312730:intron 25.0%
! ACGACTAAGATTTATTTGAA+GGG + chr8_4:9961042-9961061 Msa1312730:intron 25.0%
! AAATGTATCGTAGATTTGAT+TGG + chr8_4:9961095-9961114 Msa1312730:intron 25.0%
! CATATAAGTTTCATTGATGT+TGG + chr8_4:9961339-9961358 Msa1312730:intron 25.0%
!!! GTAGAGTTTTGTAAAATTAG+CGG + chr8_4:9961637-9961656 Msa1312730:intron 25.0%
!!! TTTTTTCAATTAAGGCTACA+AGG + chr8_4:9961720-9961739 Msa1312730:intron 25.0%
!!! TTTTTCAATTAAGGCTACAA+GGG + chr8_4:9961721-9961740 Msa1312730:intron 25.0%
! AAGCTATAAGTTCAAATATG+TGG + chr8_4:9961808-9961827 Msa1312730:intron 25.0%
! TGTTTGAACTTATATCTGTT+TGG + chr8_4:9962026-9962045 Msa1312730:intron 25.0%
! CTTGAAATATTCTAACTGTT+GGG - chr8_4:9962118-9962137 None:intergenic 25.0%
! GAATATTTCAAGGAATATCA+AGG + chr8_4:9962126-9962145 Msa1312730:CDS 25.0%
!!! CCACTTTTATCTCAAATTAA+CGG + chr8_4:9962740-9962759 Msa1312730:intron 25.0%
! TATTATCTTGTCCTTATGTT+GGG - chr8_4:9962884-9962903 None:intergenic 25.0%
! TTATTATCTTGTCCTTATGT+TGG - chr8_4:9962885-9962904 None:intergenic 25.0%
! TCAGTAAAAATAGGAAACTT+TGG - chr8_4:9963231-9963250 None:intergenic 25.0%
! CTAGAATCATCAGTAAAAAT+AGG - chr8_4:9963240-9963259 None:intergenic 25.0%
!!! CTATTTTTGGTAGTTTGTTT+TGG + chr8_4:9963368-9963387 Msa1312730:three_prime_UTR 25.0%
! TGTTCTTCATTTTCTGCTTT+TGG - chr8_4:9960632-9960651 None:intergenic 30.0%
! TTACCTTCCTACTCTTTTTA+AGG + chr8_4:9960782-9960801 Msa1312730:CDS 30.0%
TAAAATCTCTACCATAAGGA+GGG - chr8_4:9960815-9960834 None:intergenic 30.0%
CATGGAAGACCATATAATAA+AGG - chr8_4:9960946-9960965 None:intergenic 30.0%
ACATTGAGATTTCAATGAAG+AGG - chr8_4:9960969-9960988 None:intergenic 30.0%
CTTCATTGAAATCTCAATGT+TGG + chr8_4:9960969-9960988 Msa1312730:intron 30.0%
!! TCAATGTTGGTTTAACCTAA+CGG + chr8_4:9960982-9961001 Msa1312730:intron 30.0%
CGACTAAGATTTATTTGAAG+GGG + chr8_4:9961043-9961062 Msa1312730:intron 30.0%
TAAGATTTATTTGAAGGGGA+TGG + chr8_4:9961047-9961066 Msa1312730:intron 30.0%
GCTTTCTTTCTTAAATCTAG+TGG + chr8_4:9961231-9961250 Msa1312730:intron 30.0%
CCACTATAAGCTAACTTATT+AGG - chr8_4:9961581-9961600 None:intergenic 30.0%
! CCTAATAAGTTAGCTTATAG+TGG + chr8_4:9961578-9961597 Msa1312730:intron 30.0%
GAATTAAGCTATAAGGCATA+AGG + chr8_4:9961782-9961801 Msa1312730:intron 30.0%
!!! TGTTTTTGGTTTCTTCCTAT+TGG + chr8_4:9962073-9962092 Msa1312730:intron 30.0%
TATTCTAACTGTTGGGATAA+TGG - chr8_4:9962111-9962130 None:intergenic 30.0%
CCTTGAAATATTCTAACTGT+TGG - chr8_4:9962119-9962138 None:intergenic 30.0%
CCAACAGTTAGAATATTTCA+AGG + chr8_4:9962116-9962135 Msa1312730:CDS 30.0%
!! CTCATTCACAAGCTTTATTA+AGG + chr8_4:9962311-9962330 Msa1312730:intron 30.0%
TCAACAAAGAACCAACATTA+GGG - chr8_4:9962450-9962469 None:intergenic 30.0%
TTCAACAAAGAACCAACATT+AGG - chr8_4:9962451-9962470 None:intergenic 30.0%
TTATTTGGTTACCATGAGAA+TGG + chr8_4:9962555-9962574 Msa1312730:CDS 30.0%
AAAATCACAATCTTTAGACC+AGG - chr8_4:9962663-9962682 None:intergenic 30.0%
!!! AATTTGAAGGGTTTTGAACA+AGG - chr8_4:9962710-9962729 None:intergenic 30.0%
GTTCAAAACCCTTCAAATTT+CGG + chr8_4:9962711-9962730 Msa1312730:intron 30.0%
! CCGTTAATTTGAGATAAAAG+TGG - chr8_4:9962743-9962762 None:intergenic 30.0%
! AAATCAATTTAGGATGACCT+CGG - chr8_4:9962766-9962785 None:intergenic 30.0%
TAATATGAACTGTCTGATCT+TGG + chr8_4:9962796-9962815 Msa1312730:intron 30.0%
CATAAGGACAAGATAATAAC+TGG + chr8_4:9962886-9962905 Msa1312730:intron 30.0%
GCTGATAACTTAATTATTGC+CGG + chr8_4:9963113-9963132 Msa1312730:CDS 30.0%
AAAATGACACGTATTGAAGG+AGG - chr8_4:9960561-9960580 None:intergenic 35.0%
!!! CCTTCAATACGTGTCATTTT+CGG + chr8_4:9960561-9960580 Msa1312730:five_prime_UTR 35.0%
!!! CTTCAATACGTGTCATTTTC+GGG + chr8_4:9960562-9960581 Msa1312730:five_prime_UTR 35.0%
CAGTGTATGCTCAGTATATA+TGG - chr8_4:9960592-9960611 None:intergenic 35.0%
GTACCAGCTATCATAACATT+TGG + chr8_4:9960729-9960748 Msa1312730:CDS 35.0%
TCACCAAATGTTATGATAGC+TGG - chr8_4:9960735-9960754 None:intergenic 35.0%
! TAGCCTTAAAAAGAGTAGGA+AGG - chr8_4:9960788-9960807 None:intergenic 35.0%
GTAAAATCTCTACCATAAGG+AGG - chr8_4:9960816-9960835 None:intergenic 35.0%
! AAGTTAGCTACGGATTTTAC+AGG + chr8_4:9960870-9960889 Msa1312730:CDS 35.0%
GAATTGAAATATCAGCCGTT+AGG - chr8_4:9961000-9961019 None:intergenic 35.0%
TTTATTTGAAGGGGATGGAA+AGG + chr8_4:9961052-9961071 Msa1312730:intron 35.0%
ACGATACATTTCCACAAGAT+TGG - chr8_4:9961135-9961154 None:intergenic 35.0%
GTGGAAATGTATCGTAGATT+TGG - chr8_4:9961091-9961110 None:intergenic 35.0%
CGATACATTTCCACAAGATT+TGG + chr8_4:9961136-9961155 Msa1312730:intron 35.0%
! TTCCACAAGATTTGGTTGAA+TGG + chr8_4:9961144-9961163 Msa1312730:intron 35.0%
! AAGATTTGGTTGAATGGACT+TGG + chr8_4:9961150-9961169 Msa1312730:intron 35.0%
GCGTATCTCTCTAAATGTAA+CGG - chr8_4:9961213-9961232 None:intergenic 35.0%
! GATCCTCTTCATCTGAAATA+CGG - chr8_4:9961274-9961293 None:intergenic 35.0%
TGCATCTGGTTATGATGAAA+AGG + chr8_4:9961473-9961492 Msa1312730:CDS 35.0%
AGTTTCAATAAGGGTCAGTT+TGG - chr8_4:9961838-9961857 None:intergenic 35.0%
! CGGCTTGATAGTTTCAATAA+GGG - chr8_4:9961847-9961866 None:intergenic 35.0%
! ACGGCTTGATAGTTTCAATA+AGG - chr8_4:9961848-9961867 None:intergenic 35.0%
AGGAGAAATCAAGTTACTAG+AGG - chr8_4:9961996-9962015 None:intergenic 35.0%
AAAGGCTGCATCTATTATCA+AGG + chr8_4:9962176-9962195 Msa1312730:CDS 35.0%
TCTTTGTATGTATTGAGTGC+TGG + chr8_4:9962201-9962220 Msa1312730:CDS 35.0%
AATGCAAACAACCCTAATGT+TGG + chr8_4:9962436-9962455 Msa1312730:intron 35.0%
AATCTTCAAAAGCAACTTCC+TGG + chr8_4:9962642-9962661 Msa1312730:CDS 35.0%
! TTTTGAACAAGGTCAAAGAG+AGG - chr8_4:9962699-9962718 None:intergenic 35.0%
!!! AATGTTTTTTCTCCACTGCA+CGG + chr8_4:9962849-9962868 Msa1312730:intron 35.0%
! GTTCTATATAGGTTTTGCTG+AGG + chr8_4:9962947-9962966 Msa1312730:intron 35.0%
TTGTGCAATCCAAAATCACT+AGG + chr8_4:9963017-9963036 Msa1312730:CDS 35.0%
! GAACAAGTTCCTAGTGATTT+TGG - chr8_4:9963029-9963048 None:intergenic 35.0%
! ATGCAACTCAGTATGTGTTT+TGG + chr8_4:9963051-9963070 Msa1312730:CDS 35.0%
! TGCAACTCAGTATGTGTTTT+GGG + chr8_4:9963052-9963071 Msa1312730:CDS 35.0%
TTATGTGATAAGAGCTATGC+CGG - chr8_4:9963135-9963154 None:intergenic 35.0%
CCATGATTGCATTTCATATG+TGG - chr8_4:9963398-9963417 None:intergenic 35.0%
CCACATATGAAATGCAATCA+TGG + chr8_4:9963395-9963414 Msa1312730:three_prime_UTR 35.0%
CCGAAAATGACACGTATTGA+AGG - chr8_4:9960564-9960583 None:intergenic 40.0%
GTATCTTGCTTCCTAACTTG+TGG + chr8_4:9960684-9960703 Msa1312730:CDS 40.0%
AAGGCTAATTACCCTCCTTA+TGG + chr8_4:9960801-9960820 Msa1312730:CDS 40.0%
CTAATAAACAACCTACTGGG+AGG + chr8_4:9960835-9960854 Msa1312730:CDS 40.0%
TTGCAATGGCAAGTTAGCTA+CGG + chr8_4:9960860-9960879 Msa1312730:CDS 40.0%
GAGATTTCAATGAAGAGGCA+TGG - chr8_4:9960964-9960983 None:intergenic 40.0%
CATTTCCACAAGATTGGACT+TGG - chr8_4:9961080-9961099 None:intergenic 40.0%
GTCCATTCAACCAAATCTTG+TGG - chr8_4:9961149-9961168 None:intergenic 40.0%
!! AATACGGTGCTACAGCAATA+GGG - chr8_4:9961258-9961277 None:intergenic 40.0%
!! AAATACGGTGCTACAGCAAT+AGG - chr8_4:9961259-9961278 None:intergenic 40.0%
! TCTTTTGCAGCTGAAACACT+TGG + chr8_4:9961369-9961388 Msa1312730:intron 40.0%
! CTTTTGCAGCTGAAACACTT+GGG + chr8_4:9961370-9961389 Msa1312730:intron 40.0%
AAGCAAAGTTAGCACCAAGA+AGG - chr8_4:9961449-9961468 None:intergenic 40.0%
GACGTTATTTAAGTCGTGCT+TGG + chr8_4:9961539-9961558 Msa1312730:intron 40.0%
!! TATAGTGGATGAGCTGATAG+CGG + chr8_4:9961593-9961612 Msa1312730:intron 40.0%
CAAGGAATATCAAGGCAAGT+TGG + chr8_4:9962134-9962153 Msa1312730:CDS 40.0%
ACTGATCAACAGTGATTGCT+TGG - chr8_4:9962272-9962291 None:intergenic 40.0%
AGCTTGTGAATGAGTCTAAC+AGG - chr8_4:9962305-9962324 None:intergenic 40.0%
! TTGGTTCTTTGTTGAAACGC+AGG + chr8_4:9962455-9962474 Msa1312730:intron 40.0%
TGGTTCTTTGTTGAAACGCA+GGG + chr8_4:9962456-9962475 Msa1312730:intron 40.0%
GGATTAGGAGCTAGGAAAAT+TGG + chr8_4:9962486-9962505 Msa1312730:intron 40.0%
AGAATCAACACTGATGCACA+AGG + chr8_4:9962588-9962607 Msa1312730:CDS 40.0%
TTATCTCAAATTAACGGCCG+AGG + chr8_4:9962746-9962765 Msa1312730:intron 40.0%
! TATATAGGTTTTGCTGAGGC+AGG + chr8_4:9962951-9962970 Msa1312730:intron 40.0%
! ATATAGGTTTTGCTGAGGCA+GGG + chr8_4:9962952-9962971 Msa1312730:intron 40.0%
ACTTGATTAGCAGCTTCTGA+AGG - chr8_4:9963089-9963108 None:intergenic 40.0%
GGCATAGCTCTTATCACATA+AGG + chr8_4:9963134-9963153 Msa1312730:CDS 40.0%
TGACACGTATTGAAGGAGGA+AGG - chr8_4:9960557-9960576 None:intergenic 45.0%
GTGCAAAGCTACCACAAGTT+AGG - chr8_4:9960698-9960717 None:intergenic 45.0%
AGGATCCAAGTCCAATCTTG+TGG + chr8_4:9961072-9961091 Msa1312730:intron 45.0%
TAACAACGCTGCTGGTATAG+AGG - chr8_4:9961178-9961197 None:intergenic 45.0%
GCACCGTATTTCAGATGAAG+AGG + chr8_4:9961268-9961287 Msa1312730:intron 45.0%
ATCCAAGTCCTACCTAAGGT+TGG + chr8_4:9961291-9961310 Msa1312730:intron 45.0%
CATCTACTCCAACCTTAGGT+AGG - chr8_4:9961302-9961321 None:intergenic 45.0%
GCAACATCTACTCCAACCTT+AGG - chr8_4:9961306-9961325 None:intergenic 45.0%
ACCTGCATATCTTAGTCCAC+AGG + chr8_4:9961407-9961426 Msa1312730:CDS 45.0%
TATCTTAGTCCACAGGCATC+AGG + chr8_4:9961414-9961433 Msa1312730:CDS 45.0%
ATCTTAGTCCACAGGCATCA+GGG + chr8_4:9961415-9961434 Msa1312730:CDS 45.0%
CAAAGTTAGCACCAAGAAGG+AGG - chr8_4:9961446-9961465 None:intergenic 45.0%
AACTTTGCTTCAGCTGCATC+TGG + chr8_4:9961459-9961478 Msa1312730:CDS 45.0%
TGGAATTGCATGCTGCCAAT+AGG - chr8_4:9962091-9962110 None:intergenic 45.0%
! ATATCAAGGCAAGTTGGCTC+AGG + chr8_4:9962140-9962159 Msa1312730:CDS 45.0%
! TCAGGTTGCTGGCAGTAAAA+AGG + chr8_4:9962158-9962177 Msa1312730:CDS 45.0%
TGCTTGGTTGATCCAAGGAT+TGG - chr8_4:9962256-9962275 None:intergenic 45.0%
GTGATTGCTTGGTTGATCCA+AGG - chr8_4:9962261-9962280 None:intergenic 45.0%
ATAAAGTCCTTGCAGCAGGT+AGG - chr8_4:9962539-9962558 None:intergenic 45.0%
CCAAATAAAGTCCTTGCAGC+AGG - chr8_4:9962543-9962562 None:intergenic 45.0%
CCTGCTGCAAGGACTTTATT+TGG + chr8_4:9962540-9962559 Msa1312730:intron 45.0%
TTGCGACACAACCATTCTCA+TGG - chr8_4:9962569-9962588 None:intergenic 45.0%
GGTGCGTACCGAAATTTGAA+GGG - chr8_4:9962722-9962741 None:intergenic 45.0%
TGGTGCGTACCGAAATTTGA+AGG - chr8_4:9962723-9962742 None:intergenic 45.0%
! TTACGCGCAGTTTTCGATCT+CGG - chr8_4:9962830-9962849 None:intergenic 45.0%
TTTCTCCACTGCACGGTATT+CGG + chr8_4:9962856-9962875 Msa1312730:intron 45.0%
GGTATTCGGATCCCAACATA+AGG + chr8_4:9962870-9962889 Msa1312730:intron 45.0%
!!! AATTTTCAAAAAAATTAAAA+CGG - chr8_4:9961867-9961886 None:intergenic 5.0%
CACGTATTGAAGGAGGAAGG+TGG - chr8_4:9960554-9960573 None:intergenic 50.0%
GGTGACTCTGCAGTAGATGT+AGG + chr8_4:9960750-9960769 Msa1312730:CDS 50.0%
CCATTGCAAAACCTCCCAGT+AGG - chr8_4:9960849-9960868 None:intergenic 50.0%
! CCTACTGGGAGGTTTTGCAA+TGG + chr8_4:9960846-9960865 Msa1312730:CDS 50.0%
TACAGCTGTAACAACGCTGC+TGG - chr8_4:9961186-9961205 None:intergenic 50.0%
AGCAGCGTTGTTACAGCTGT+AGG + chr8_4:9961185-9961204 Msa1312730:intron 50.0%
GAGGATCCAAGTCCTACCTA+AGG + chr8_4:9961287-9961306 Msa1312730:intron 50.0%
CTCCAACCTTAGGTAGGACT+TGG - chr8_4:9961296-9961315 None:intergenic 50.0%
! GCCTGTGGACTAAGATATGC+AGG - chr8_4:9961411-9961430 None:intergenic 50.0%
TCAGGGAAGAACCTCCTTCT+TGG + chr8_4:9961432-9961451 Msa1312730:CDS 50.0%
!! GCGTGTATGGATTAGGAGCT+AGG + chr8_4:9962478-9962497 Msa1312730:intron 50.0%
AGTGACCTCACTTCCACCAT+TGG + chr8_4:9962509-9962528 Msa1312730:intron 50.0%
GTGACCTCACTTCCACCATT+GGG + chr8_4:9962510-9962529 Msa1312730:intron 50.0%
! GTTTTGCTGAGGCAGGGAAA+GGG + chr8_4:9962958-9962977 Msa1312730:CDS 50.0%
AGGTTCTTCCCTGATGCCTG+TGG - chr8_4:9961426-9961445 None:intergenic 55.0%
GTTGAAACGCAGGGCGTGTA+TGG + chr8_4:9962465-9962484 Msa1312730:intron 55.0%
ACGCAGGGCGTGTATGGATT+AGG + chr8_4:9962471-9962490 Msa1312730:intron 55.0%
! GCAACCCAATGGTGGAAGTG+AGG - chr8_4:9962517-9962536 None:intergenic 55.0%
! GGTTTTGCTGAGGCAGGGAA+AGG + chr8_4:9962957-9962976 Msa1312730:intron 55.0%
! GGCAAGTTGGCTCAGGTTGC+TGG + chr8_4:9962147-9962166 Msa1312730:CDS 60.0%
GCAGGTAGGCAACCCAATGG+TGG - chr8_4:9962525-9962544 None:intergenic 60.0%
GCAGCAGGTAGGCAACCCAA+TGG - chr8_4:9962528-9962547 None:intergenic 60.0%
TGGGTTGCCTACCTGCTGCA+AGG + chr8_4:9962529-9962548 Msa1312730:intron 60.0%
GGGATCCGAATACCGTGCAG+TGG - chr8_4:9962864-9962883 None:intergenic 60.0%
!! GGGAAAGGGTGCTGTGGTAC+AGG + chr8_4:9962972-9962991 Msa1312730:CDS 60.0%
!! GAGGCAGGGAAAGGGTGCTG+TGG + chr8_4:9962966-9962985 Msa1312730:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 9960544 9963431 9960544 ID=Msa1312730;Name=Msa1312730
chr8_4 mRNA 9960544 9963431 9960544 ID=Msa1312730-mRNA-1;Parent=Msa1312730;Name=Msa1312730-mRNA-1;_AED=0.09;_eAED=0.09;_QI=98|0.5|0.6|1|0.5|0.6|5|277|298
chr8_4 exon 9960544 9960891 9960544 ID=Msa1312730-mRNA-1:exon:3731;Parent=Msa1312730-mRNA-1
chr8_4 exon 9961379 9961509 9961379 ID=Msa1312730-mRNA-1:exon:3732;Parent=Msa1312730-mRNA-1
chr8_4 exon 9962099 9962304 9962099 ID=Msa1312730-mRNA-1:exon:3733;Parent=Msa1312730-mRNA-1
chr8_4 exon 9962551 9962663 9962551 ID=Msa1312730-mRNA-1:exon:3734;Parent=Msa1312730-mRNA-1
chr8_4 exon 9962958 9963431 9962958 ID=Msa1312730-mRNA-1:exon:3735;Parent=Msa1312730-mRNA-1
chr8_4 five_prime_UTR 9960544 9960641 9960544 ID=Msa1312730-mRNA-1:five_prime_utr;Parent=Msa1312730-mRNA-1
chr8_4 CDS 9960642 9960891 9960642 ID=Msa1312730-mRNA-1:cds;Parent=Msa1312730-mRNA-1
chr8_4 CDS 9961379 9961509 9961379 ID=Msa1312730-mRNA-1:cds;Parent=Msa1312730-mRNA-1
chr8_4 CDS 9962099 9962304 9962099 ID=Msa1312730-mRNA-1:cds;Parent=Msa1312730-mRNA-1
chr8_4 CDS 9962551 9962663 9962551 ID=Msa1312730-mRNA-1:cds;Parent=Msa1312730-mRNA-1
chr8_4 CDS 9962958 9963154 9962958 ID=Msa1312730-mRNA-1:cds;Parent=Msa1312730-mRNA-1
chr8_4 three_prime_UTR 9963155 9963431 9963155 ID=Msa1312730-mRNA-1:three_prime_utr;Parent=Msa1312730-mRNA-1
Gene Sequence

>Msa1312730

ATGAAGAACATGAATAGCAAACAAACACTTGTTCTACTCATAGTATCTTGCTTCCTAACTTGTGGTAGCTTTGCACAAGATACACTTGTACCAGCTATCATAACATTTGGTGACTCTGCAGTAGATGTAGGAAATAATGATTACCTTCCTACTCTTTTTAAGGCTAATTACCCTCCTTATGGTAGAGATTTTACTAATAAACAACCTACTGGGAGGTTTTGCAATGGCAAGTTAGCTACGGATTTTACAGCTGAAACACTTGGGTTTACGAGTTTTGCACCTGCATATCTTAGTCCACAGGCATCAGGGAAGAACCTCCTTCTTGGTGCTAACTTTGCTTCAGCTGCATCTGGTTATGATGAAAAGGCTGCTACGTTGAATCATGCAATTCCATTATCCCAACAGTTAGAATATTTCAAGGAATATCAAGGCAAGTTGGCTCAGGTTGCTGGCAGTAAAAAGGCTGCATCTATTATCAAGGATTCTTTGTATGTATTGAGTGCTGGCAGCAGTGACTTTGTACAAAATTATTATACCAATCCTTGGATCAACCAAGCAATCACTGTTGATCAGTATTCATCTTACCTGACTTTATTTGGTTACCATGAGAATGGTTGTGTCGCAAGAATCAACACTGATGCACAAGGATTTAACAAGAAAGTCAGCTCAGCTGCATCGAATCTTCAAAAGCAACTTCCTGGTTTTGCTGAGGCAGGGAAAGGGTGCTGTGGTACAGGTCTTGTAGAAACAACATCATTATTGTGCAATCCAAAATCACTAGGAACTTGTTCTAATGCAACTCAGTATGTGTTTTGGGACAGTGTTCATCCTTCAGAAGCTGCTAATCAAGTTCTAGCTGATAACTTAATTATTGCCGGCATAGCTCTTATCACATAA
Protein sequence

>Msa1312730

MKNMNSKQTLVLLIVSCFLTCGSFAQDTLVPAIITFGDSAVDVGNNDYLPTLFKANYPPYGRDFTNKQPTGRFCNGKLATDFTAETLGFTSFAPAYLSPQASGKNLLLGANFASAASGYDEKAATLNHAIPLSQQLEYFKEYQGKLAQVAGSKKAASIIKDSLYVLSAGSSDFVQNYYTNPWINQAITVDQYSSYLTLFGYHENGCVARINTDAQGFNKKVSSAASNLQKQLPGFAEAGKGCCGTGLVETTSLLCNPKSLGTCSNATQYVFWDSVHPSEAANQVLADNLIIAGIALIT*