AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0065390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0065390 MtrunA17_Chr1g0168601 73.226 310 7 2 1 310 1 234 1.04e-155 434
Msa0065390 MtrunA17_Chr3g0116631 33.770 305 168 4 10 308 9 285 4.28e-44 152
Msa0065390 MtrunA17_Chr4g0058151 46.795 156 80 1 115 267 123 278 7.01e-43 149
Msa0065390 MtrunA17_Chr4g0058151 48.780 82 41 1 115 196 217 297 2.76e-19 86.3
Msa0065390 MtrunA17_Chr7g0267571 29.721 323 184 6 1 310 1 293 4.30e-41 145
Msa0065390 MtrunA17_Chr1g0181111 32.827 329 141 10 2 308 5 275 4.75e-39 139
Msa0065390 MtrunA17_Chr1g0177101 36.190 210 102 2 108 309 102 287 6.04e-37 134
Msa0065390 MtrunA17_Chr3g0087481 40.741 162 88 1 115 268 61 222 1.32e-36 131
Msa0065390 MtrunA17_Chr2g0328791 30.168 179 119 3 116 288 17 195 3.42e-18 84.3
Msa0065390 MtrunA17_Chr5g0411091 36.242 149 88 3 111 253 93 240 6.54e-18 82.8
Msa0065390 MtrunA17_Chr3g0132451 31.447 159 94 5 114 263 89 241 3.12e-16 77.4
Msa0065390 MtrunA17_Chr4g0035001 43.284 67 38 0 201 267 2 68 1.17e-15 73.2
Msa0065390 MtrunA17_Chr4g0074711 29.936 157 107 2 116 269 76 232 1.55e-15 76.6
Msa0065390 MtrunA17_Chr3g0110211 30.952 168 111 2 101 267 12 175 2.07e-15 75.9
Msa0065390 MtrunA17_Chr4g0035051 37.805 82 47 1 201 278 4 85 4.66e-15 70.5
Msa0065390 MtrunA17_Chr7g0276201 29.146 199 116 4 74 269 74 250 6.79e-15 75.1
Msa0065390 MtrunA17_Chr4g0061451 40.964 83 48 1 114 196 42 123 9.47e-15 72.8
Msa0065390 MtrunA17_Chr7g0219021 40.244 82 48 1 115 195 41 122 1.29e-14 72.4
Msa0065390 MtrunA17_Chr7g0219021 33.000 100 43 1 206 305 41 116 6.74e-12 64.7
Msa0065390 MtrunA17_Chr3g0119311 37.805 82 50 1 114 194 36 117 6.48e-14 67.8
Msa0065390 MtrunA17_Chr3g0119311 43.548 62 35 0 206 267 37 98 5.35e-12 62.4
Msa0065390 MtrunA17_Chr4g0035031 39.759 83 46 1 201 279 4 86 7.37e-14 68.9
Msa0065390 MtrunA17_Chr4g0070231 27.485 171 112 3 116 286 48 206 1.32e-13 70.9
Msa0065390 MtrunA17_Chr4g0045221 27.907 172 115 4 107 270 110 280 1.44e-13 70.9
Msa0065390 MtrunA17_Chr2g0288251 28.333 180 104 4 105 269 51 220 3.10e-13 69.7
Msa0065390 MtrunA17_Chr3g0086861 37.647 85 52 1 113 197 43 126 3.13e-13 69.7
Msa0065390 MtrunA17_Chr1g0190211 44.118 68 38 0 200 267 127 194 1.21e-12 66.6
Msa0065390 MtrunA17_Chr1g0153871 29.114 158 109 2 116 270 99 256 1.43e-12 67.8
Msa0065390 MtrunA17_Chr3g0139111 28.477 151 102 2 116 266 62 206 2.12e-12 67.4
Msa0065390 MtrunA17_Chr8g0375101 28.042 189 119 6 115 295 5 184 2.75e-12 66.6
Msa0065390 MtrunA17_Chr7g0219031 36.842 76 48 0 193 268 28 103 3.34e-12 62.4
Msa0065390 MtrunA17_Chr1g0179701 28.846 156 108 3 113 267 149 302 3.39e-12 67.0
Msa0065390 MtrunA17_Chr5g0402191 31.250 144 90 4 115 255 5 142 3.67e-12 66.6
Msa0065390 MtrunA17_Chr4g0050781 32.381 105 47 1 202 306 4 84 6.31e-12 63.9
Msa0065390 MtrunA17_Chr5g0447761 28.916 166 93 3 115 263 7 164 9.72e-12 65.5
Msa0065390 MtrunA17_Chr6g0465751 26.752 157 109 3 114 269 25 176 9.93e-12 65.5
Msa0065390 MtrunA17_Chr3g0101061 30.909 165 91 5 115 263 41 198 1.17e-11 65.1
Msa0065390 MtrunA17_Chr8g0344491 27.485 171 112 4 116 286 64 222 1.45e-11 64.7
Msa0065390 MtrunA17_Chr6g0466501 27.097 155 107 3 116 269 27 176 1.66e-11 64.7
Msa0065390 MtrunA17_Chr3g0121631 34.286 105 44 2 206 310 37 116 1.66e-11 61.2
Msa0065390 MtrunA17_Chr3g0121631 37.179 78 48 1 114 191 36 112 6.22e-11 59.7
Msa0065390 MtrunA17_Chr2g0278661 31.068 103 47 1 204 306 6 84 2.25e-11 62.8
Msa0065390 MtrunA17_Chr1g0212611 27.950 161 112 3 111 269 217 375 2.53e-11 64.3
Msa0065390 MtrunA17_Chr7g0266751 44.444 63 35 0 205 267 32 94 2.62e-11 60.5
Msa0065390 MtrunA17_Chr3g0120721 29.412 153 97 3 115 263 19 164 7.34e-11 62.4
Msa0065390 MtrunA17_Chr2g0290191 27.632 152 95 3 115 253 5 154 8.08e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0065390 AT1G60000.1 58.515 229 61 4 82 309 62 257 2.08e-86 260
Msa0065390 AT5G50250.1 43.017 179 94 3 93 267 95 269 1.48e-42 148
Msa0065390 AT5G50250.1 43.902 82 46 0 111 192 204 285 1.66e-15 75.9
Msa0065390 AT2G37220.1 37.037 216 90 3 115 308 92 283 1.02e-39 141
Msa0065390 AT2G37220.1 38.554 83 51 0 114 196 204 286 5.62e-13 68.6
Msa0065390 AT4G24770.1 38.776 196 91 3 115 306 151 321 2.80e-39 141
Msa0065390 AT4G24770.1 47.561 82 43 0 115 196 245 326 1.75e-19 87.8
Msa0065390 AT4G24770.2 38.776 196 91 3 115 306 151 321 2.80e-39 141
Msa0065390 AT4G24770.2 47.561 82 43 0 115 196 245 326 1.75e-19 87.8
Msa0065390 AT3G52150.2 39.634 164 89 1 115 268 77 240 8.44e-38 135
Msa0065390 AT3G52150.1 39.634 164 89 1 115 268 77 240 8.44e-38 135
Msa0065390 AT3G52380.1 33.498 203 99 2 115 305 117 295 1.43e-36 134
Msa0065390 AT3G52380.1 33.077 130 76 4 81 204 188 312 3.47e-11 63.5
Msa0065390 AT1G01080.3 35.583 163 92 2 116 265 110 272 1.59e-27 108
Msa0065390 AT1G01080.1 35.583 163 92 2 116 265 110 272 1.60e-27 108
Msa0065390 AT2G35410.1 35.897 195 110 6 83 267 68 257 1.91e-27 109
Msa0065390 AT1G01080.2 35.366 164 92 3 116 265 110 273 2.18e-26 105
Msa0065390 AT3G53460.1 48.148 108 50 2 94 196 74 180 5.48e-19 86.3
Msa0065390 AT3G53460.1 39.759 83 50 0 114 196 257 339 2.77e-13 70.1
Msa0065390 AT3G53460.1 33.010 103 45 1 206 308 258 336 6.52e-12 65.9
Msa0065390 AT3G53460.3 48.148 108 50 2 94 196 74 180 5.48e-19 86.3
Msa0065390 AT3G53460.3 39.759 83 50 0 114 196 257 339 2.77e-13 70.1
Msa0065390 AT3G53460.3 33.010 103 45 1 206 308 258 336 6.52e-12 65.9
Msa0065390 AT3G53460.2 48.148 108 50 2 94 196 74 180 5.48e-19 86.3
Msa0065390 AT3G53460.2 39.759 83 50 0 114 196 257 339 2.77e-13 70.1
Msa0065390 AT3G53460.2 33.010 103 45 1 206 308 258 336 6.52e-12 65.9
Msa0065390 AT4G09040.1 32.121 165 105 3 105 263 85 248 1.68e-17 81.6
Msa0065390 AT4G09040.2 31.646 158 101 3 112 263 32 188 3.10e-17 80.1
Msa0065390 AT5G19350.1 30.178 169 111 4 105 270 18 182 9.81e-16 77.8
Msa0065390 AT5G19350.2 30.178 169 111 4 105 270 18 182 1.10e-15 77.4
Msa0065390 AT3G53460.4 40.310 129 50 3 94 196 74 201 4.70e-15 75.5
Msa0065390 AT3G53460.4 39.759 83 50 0 114 196 278 360 3.40e-13 69.7
Msa0065390 AT3G53460.4 33.010 103 45 1 206 308 279 357 7.22e-12 65.9
Msa0065390 AT3G23830.2 41.558 77 45 0 114 190 35 111 7.99e-15 70.9
Msa0065390 AT3G23830.2 38.000 100 38 1 206 305 36 111 4.93e-12 62.8
Msa0065390 AT3G23830.1 41.558 77 45 0 114 190 35 111 7.99e-15 70.9
Msa0065390 AT3G23830.1 38.000 100 38 1 206 305 36 111 4.93e-12 62.8
Msa0065390 AT5G54900.1 28.571 175 121 3 97 267 43 217 1.61e-14 73.9
Msa0065390 AT1G11650.1 28.743 167 96 4 116 269 64 220 2.70e-14 72.4
Msa0065390 AT4G00830.2 28.249 177 106 4 114 280 116 281 3.95e-14 73.2
Msa0065390 AT4G00830.1 28.249 177 106 4 114 280 116 281 3.95e-14 73.2
Msa0065390 AT4G00830.6 28.249 177 106 4 114 280 116 281 3.95e-14 73.2
Msa0065390 AT1G11650.2 28.743 167 96 4 116 269 64 220 9.95e-14 71.6
Msa0065390 AT4G00830.3 27.429 175 102 3 114 280 116 273 1.40e-13 71.2
Msa0065390 AT4G00830.5 27.429 175 102 3 114 280 116 273 1.40e-13 71.2
Msa0065390 AT1G54080.1 29.412 153 100 4 116 267 65 210 2.69e-13 70.5
Msa0065390 AT5G61030.2 40.000 80 48 0 112 191 38 117 2.83e-13 69.7
Msa0065390 AT5G61030.1 40.000 80 48 0 112 191 38 117 2.83e-13 69.7
Msa0065390 AT3G14100.1 28.289 152 103 2 116 267 61 206 3.16e-13 70.1
Msa0065390 AT3G26420.1 32.381 105 47 1 202 306 4 84 7.39e-13 67.8
Msa0065390 AT4G39260.4 38.806 67 41 0 201 267 2 68 8.95e-13 64.3
Msa0065390 AT4G39260.3 38.806 67 41 0 201 267 2 68 1.19e-12 63.5
Msa0065390 AT4G39260.2 38.806 67 41 0 201 267 2 68 1.24e-12 64.3
Msa0065390 AT4G13850.3 40.000 85 50 1 114 198 35 118 1.44e-12 64.7
Msa0065390 AT4G13850.3 46.774 62 33 0 206 267 36 97 2.90e-12 63.9
Msa0065390 AT5G40490.1 26.486 185 127 4 83 263 9 188 1.58e-12 68.2
Msa0065390 AT4G36960.1 27.485 171 110 6 115 280 4 165 1.64e-12 67.8
Msa0065390 AT4G36960.2 27.485 171 110 6 115 280 4 165 1.64e-12 67.8
Msa0065390 AT4G13850.2 40.000 85 50 1 114 198 35 118 1.74e-12 64.7
Msa0065390 AT4G13850.2 46.774 62 33 0 206 267 36 97 3.51e-12 63.9
Msa0065390 AT4G13850.1 40.000 85 50 1 114 198 35 118 1.93e-12 64.7
Msa0065390 AT4G13850.1 46.774 62 33 0 206 267 36 97 3.89e-12 63.9
Msa0065390 AT2G18510.1 29.341 167 113 2 101 266 12 174 2.21e-12 67.4
Msa0065390 AT1G17370.2 28.378 148 100 2 116 263 56 197 2.27e-12 67.4
Msa0065390 AT4G13850.4 41.975 81 46 1 114 193 35 115 2.51e-12 63.5
Msa0065390 AT4G13850.4 46.774 62 33 0 206 267 36 97 2.51e-12 63.5
Msa0065390 AT1G17370.1 28.378 148 100 2 116 263 56 197 2.53e-12 67.4
Msa0065390 AT4G34110.1 26.786 168 116 4 103 269 26 187 2.84e-12 67.8
Msa0065390 AT4G34110.1 29.787 141 96 3 116 255 126 264 6.82e-11 63.5
Msa0065390 AT4G27000.1 29.677 155 106 2 116 267 82 236 3.09e-12 67.0
Msa0065390 AT4G39260.1 38.806 67 41 0 201 267 2 68 3.10e-12 64.3
Msa0065390 AT1G74230.1 40.260 77 45 1 114 190 34 109 3.71e-12 66.2
Msa0065390 AT2G36660.1 29.016 193 111 7 95 265 176 364 4.89e-12 67.0
Msa0065390 AT3G13224.1 29.114 158 95 4 115 264 20 168 4.91e-12 65.1
Msa0065390 AT2G21660.2 39.394 66 40 0 202 267 5 70 5.77e-12 63.2
Msa0065390 AT4G14300.1 30.303 165 96 4 115 263 7 168 7.46e-12 65.9
Msa0065390 AT4G14300.2 30.303 165 96 4 115 263 7 168 7.46e-12 65.9
Msa0065390 AT3G04500.1 44.118 68 38 0 200 267 132 199 7.95e-12 64.7
Msa0065390 AT2G21660.1 39.394 66 40 0 202 267 5 70 7.97e-12 63.2
Msa0065390 AT2G23350.1 29.358 218 126 5 64 267 186 389 1.03e-11 65.9
Msa0065390 AT3G08000.1 42.857 63 36 0 206 268 42 104 2.03e-11 61.2
Msa0065390 AT3G08000.1 36.842 76 48 0 115 190 42 117 7.24e-11 59.7
Msa0065390 AT1G54080.2 28.662 157 100 5 116 267 65 214 2.08e-11 64.7
Msa0065390 AT4G13860.1 45.161 62 34 0 206 267 4 65 3.92e-11 58.9
Msa0065390 AT3G19130.1 28.659 164 112 4 111 270 106 268 3.94e-11 63.9
Msa0065390 AT3G16380.1 28.324 173 96 5 114 267 202 365 4.51e-11 63.9
Msa0065390 AT3G16380.1 31.410 156 100 4 112 263 107 259 7.57e-11 63.2
Msa0065390 AT5G55550.2 27.811 169 95 6 115 263 7 168 5.22e-11 63.5
Msa0065390 AT5G55550.3 27.811 169 95 6 115 263 7 168 5.22e-11 63.5
Msa0065390 AT5G55550.6 27.811 169 95 6 115 263 7 168 5.22e-11 63.5
Msa0065390 AT5G55550.11 27.811 169 95 6 115 263 7 168 5.22e-11 63.5
Msa0065390 AT1G47500.2 28.125 160 109 4 116 269 105 264 5.41e-11 62.8
Msa0065390 AT1G03457.1 27.219 169 113 3 105 270 3 164 5.92e-11 63.2
Msa0065390 AT5G55550.5 27.811 169 95 6 115 263 7 168 6.14e-11 63.2
Msa0065390 AT5G55550.4 27.811 169 95 6 115 263 7 168 6.14e-11 63.2
Msa0065390 AT5G55550.9 27.811 169 95 6 115 263 7 168 6.14e-11 63.2
Msa0065390 AT5G55550.7 27.811 169 95 6 115 263 7 168 6.14e-11 63.2
Msa0065390 AT5G55550.1 27.811 169 95 6 115 263 7 168 6.14e-11 63.2
Msa0065390 AT4G16280.4 24.658 219 153 6 96 309 27 238 7.08e-11 63.5
Msa0065390 AT3G13224.3 29.114 158 95 4 115 264 11 159 8.03e-11 62.8
Msa0065390 AT3G13224.2 29.114 158 95 4 115 264 20 168 8.39e-11 62.8

Find 92 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCCCCTTCTCATTTCTT+TGG 0.195436 1_2:-27473046 None:intergenic
AAAAGCTTGTACTCAGTTTC+AGG 0.198175 1_2:-27472462 None:intergenic
GGCATCCATTTCTGCTTTAT+TGG 0.203256 1_2:-27472622 None:intergenic
GCCATAGCCACGAGACTTTC+CGG 0.218687 1_2:-27472586 None:intergenic
ACTAGGACTAAGCTTTATTT+TGG 0.246763 1_2:+27471011 Msa0065390:CDS
GTCAGCATATCTTATGAAAA+TGG 0.271815 1_2:-27473152 None:intergenic
TAACACAACAAAATATCATT+TGG 0.284270 1_2:-27473175 None:intergenic
GTTGATAGTGCACTACTTGC+AGG 0.298430 1_2:+27471050 Msa0065390:CDS
TCAGGATACAATGGTTCTTT+TGG 0.300465 1_2:-27472444 None:intergenic
CAAGAACATGGAACTGTTGT+TGG 0.308779 1_2:+27472537 Msa0065390:CDS
GCAACAGCAACAGAAATTCT+AGG 0.317781 1_2:-27470858 None:intergenic
GTAGAAGACAAGGAGAATAT+TGG 0.324271 1_2:+27470963 Msa0065390:CDS
CAACAGTTTCACACAAGTTA+TGG 0.342573 1_2:+27470832 Msa0065390:CDS
GTTCATGGGGCGAACCTTGC+GGG 0.348120 1_2:+27472398 Msa0065390:CDS
GCAGGGTTAATTGAGGAGTA+TGG 0.364135 1_2:+27471068 Msa0065390:CDS
TAGACATTTGACAGTGTTGA+TGG 0.384129 1_2:-27470718 None:intergenic
AACACAACAAAATATCATTT+GGG 0.387457 1_2:-27473174 None:intergenic
CTATGCCAATAAAGCAGAAA+TGG 0.389179 1_2:+27472617 Msa0065390:CDS
CCACATTATCCATGATTGTA+AGG 0.390696 1_2:-27472647 None:intergenic
AGTGCACTATCAACACTATA+AGG 0.401443 1_2:-27471041 None:intergenic
GTGTTATATGATGGCGAAAC+CGG 0.401723 1_2:+27472567 Msa0065390:CDS
ATGACAAATATCAACATGAT+AGG 0.404200 1_2:+27473332 Msa0065390:three_prime_UTR
AACAACTTCTCAGGAGTTCA+TGG 0.409783 1_2:+27472383 Msa0065390:intron
ACCGGAAAGTCTCGTGGCTA+TGG 0.439880 1_2:+27472585 Msa0065390:CDS
AGAGAAAGTTGTAGTGGAAG+AGG 0.455192 1_2:+27470914 Msa0065390:CDS
TGTTGCTGTTGCACAGGAAG+AGG 0.459809 1_2:+27470869 Msa0065390:CDS
TCAGCATATCTTATGAAAAT+GGG 0.463816 1_2:-27473151 None:intergenic
GGATGCCGCCCTTACAATCA+TGG 0.469351 1_2:+27472638 Msa0065390:CDS
GAAGACAAGGAGAATATTGG+AGG 0.469732 1_2:+27470966 Msa0065390:CDS
AGATACAAACATGCGGTTTG+AGG 0.470629 1_2:-27470769 None:intergenic
AACAACATGGCTACTGCAGC+AGG 0.474982 1_2:+27470672 Msa0065390:exon
TGTGCACTTCAGGAACTAGA+AGG 0.476194 1_2:+27472935 Msa0065390:intron
GTTCCTTTGCATATTTCTTG+TGG 0.484461 1_2:+27473111 Msa0065390:three_prime_UTR
AATAAGTAAGATGTGATGAG+AGG 0.487271 1_2:-27470604 None:intergenic
TGCAAAGGAACTTCTTATCA+AGG 0.488376 1_2:-27473099 None:intergenic
ACGAACATTGCGAGTAAGCT+TGG 0.496136 1_2:+27472958 Msa0065390:CDS
CATTTCTGCTTTATTGGCAT+AGG 0.501951 1_2:-27472616 None:intergenic
AATTTCTGTTGCTGTTGCAC+AGG 0.504166 1_2:+27470863 Msa0065390:CDS
CATGGCTACTGCAGCAGGCA+TGG 0.506381 1_2:+27470677 Msa0065390:exon
CAGTAATTCAAAATCTTGAC+GGG 0.510605 1_2:+27472286 Msa0065390:CDS
GCAGTAATTCAAAATCTTGA+CGG 0.511117 1_2:+27472285 Msa0065390:CDS
GCTCAAGGAAAACGTTCATA+AGG 0.515842 1_2:+27472980 Msa0065390:CDS
GGATGAAGAGAAAGTTGTAG+TGG 0.517744 1_2:+27470908 Msa0065390:CDS
AACAGTTCCATGTTCTTGAA+AGG 0.519477 1_2:-27472532 None:intergenic
AGAAGAAGTAGTAGAAGACA+AGG 0.522447 1_2:+27470953 Msa0065390:CDS
TTGATATTTGTCATTTCACT+AGG 0.529510 1_2:-27473323 None:intergenic
GTTCTTTATGACAGAGACAC+TGG 0.531305 1_2:+27472210 Msa0065390:CDS
TCAGTGGAACCTTTGTCCAT+TGG 0.533846 1_2:+27470807 Msa0065390:CDS
AGTTCATGGGGCGAACCTTG+CGG 0.534379 1_2:+27472397 Msa0065390:CDS
GTGTGAAACTGTTGTGCCAA+TGG 0.548700 1_2:-27470823 None:intergenic
ATAAGTAAGATGTGATGAGA+GGG 0.554891 1_2:-27470603 None:intergenic
ACACAAGCCTTTCAAGAACA+TGG 0.556072 1_2:+27472525 Msa0065390:CDS
AGAGGCTGTTGCGGCTGTTG+AGG 0.559165 1_2:+27470887 Msa0065390:CDS
ATGGTGCCAAAGAAATGAGA+AGG 0.560077 1_2:+27473040 Msa0065390:three_prime_UTR
ATGGAACTGTTGTTGGAGCT+AGG 0.560429 1_2:+27472544 Msa0065390:CDS
ACAACTTCTCAGGAGTTCAT+GGG 0.561128 1_2:+27472384 Msa0065390:intron
TACTCAGTTTCAGGATACAA+TGG 0.561289 1_2:-27472453 None:intergenic
ACTACTTGCAGGGTTAATTG+AGG 0.562878 1_2:+27471061 Msa0065390:CDS
CTGTAGCAGAAGTAGACACT+AGG 0.564886 1_2:+27470994 Msa0065390:CDS
AAGACAAGGAGAATATTGGA+GGG 0.565825 1_2:+27470967 Msa0065390:CDS
AGACAAGGAGAATATTGGAG+GGG 0.580888 1_2:+27470968 Msa0065390:CDS
TTGATAGTGCACTACTTGCA+GGG 0.581102 1_2:+27471051 Msa0065390:CDS
TGGTGCCAAAGAAATGAGAA+GGG 0.589235 1_2:+27473041 Msa0065390:three_prime_UTR
CCTTACAATCATGGATAATG+TGG 0.592838 1_2:+27472647 Msa0065390:CDS
TGGAACTGTTGTTGGAGCTA+GGG 0.592911 1_2:+27472545 Msa0065390:CDS
CACATTATCCATGATTGTAA+GGG 0.601934 1_2:-27472646 None:intergenic
GTCAGCGAAATTCACCCGCA+AGG 0.601960 1_2:-27472412 None:intergenic
GTTTGAGGATGTATCTAATG+AGG 0.603592 1_2:-27470754 None:intergenic
ATTATCCATGATTGTAAGGG+CGG 0.604033 1_2:-27472643 None:intergenic
TGAAGCAAGTGCATCATGGT+AGG 0.604143 1_2:-27473076 None:intergenic
GCAATTATATGCAAATGTCA+AGG 0.604974 1_2:+27472817 Msa0065390:CDS
AGGTCGATATAAACTCGGAA+AGG 0.610410 1_2:+27473000 Msa0065390:exon
TGGTAGTGCAGAATTAATTG+AGG 0.611201 1_2:+27471088 Msa0065390:CDS
AGTGCATCATGGTAGGTACA+TGG 0.614495 1_2:-27473069 None:intergenic
AAGGTGAAGCAAGTGCATCA+TGG 0.618648 1_2:-27473080 None:intergenic
TTGCGAGTAAGCTTGGCTCA+AGG 0.622339 1_2:+27472965 Msa0065390:CDS
CCAAAGAAATGAGAAGGGGA+TGG 0.634257 1_2:+27473046 Msa0065390:three_prime_UTR
GGAGCTAGGGTGTTATATGA+TGG 0.637064 1_2:+27472558 Msa0065390:CDS
GGCGAAACCGGAAAGTCTCG+TGG 0.640311 1_2:+27472579 Msa0065390:CDS
CAACTTCTCAGGAGTTCATG+GGG 0.648152 1_2:+27472385 Msa0065390:intron
TCATAAGGTCGATATAAACT+CGG 0.654500 1_2:+27472995 Msa0065390:exon
GGCTCATTGATGTTCAACAA+TGG 0.655669 1_2:+27473021 Msa0065390:three_prime_UTR
ACACCACAAGAAATATGCAA+AGG 0.657723 1_2:-27473114 None:intergenic
TGCACAGGAAGAGGCTGTTG+CGG 0.659983 1_2:+27470878 Msa0065390:CDS
CCACTGTCATCACAACAACA+TGG 0.664017 1_2:+27470659 Msa0065390:exon
TTTGATCTCACAATCCTCAG+TGG 0.666985 1_2:+27470791 Msa0065390:CDS
ACTGTTGTGCCAATGGACAA+AGG 0.686508 1_2:-27470816 None:intergenic
GGTGCCAAAGAAATGAGAAG+GGG 0.702569 1_2:+27473042 Msa0065390:three_prime_UTR
CCATGTTGTTGTGATGACAG+TGG 0.702987 1_2:-27470659 None:intergenic
AGATCAAAGATACAAACATG+CGG 0.717953 1_2:-27470776 None:intergenic
GATGGAAGATGAAAACAATG+AGG 0.726129 1_2:-27470700 None:intergenic
AATGGACAAAGGTTCCACTG+AGG 0.804834 1_2:-27470805 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTATTTTAATATTCTTC+AGG + chr1_2:27471640-27471659 Msa0065390:intron 10.0%
!! TAAACTATAAAACTTAGTTA+TGG + chr1_2:27472013-27472032 Msa0065390:intron 15.0%
!! TATACAAATCATTAACATTA+CGG - chr1_2:27472181-27472200 None:intergenic 15.0%
!!! TGAAGTAATTCTATTAGTTT+TGG + chr1_2:27471382-27471401 Msa0065390:intron 20.0%
!! TACTATCTATATGATGATTT+AGG + chr1_2:27471440-27471459 Msa0065390:intron 20.0%
!! TTAAAATTAACAGGTCTTAA+TGG - chr1_2:27471746-27471765 None:intergenic 20.0%
!! CTTTAATGAATTGTAAATTC+AGG - chr1_2:27471856-27471875 None:intergenic 20.0%
!!! CTATCGATTATAAATTTAGT+TGG - chr1_2:27471909-27471928 None:intergenic 20.0%
!!! GTAATTGTGATTTAAAACTT+CGG + chr1_2:27472854-27472873 Msa0065390:intron 20.0%
!! AACACAACAAAATATCATTT+GGG - chr1_2:27473177-27473196 None:intergenic 20.0%
!! TAACACAACAAAATATCATT+TGG - chr1_2:27473178-27473197 None:intergenic 20.0%
! AATATTCATCGTTAAGAACT+AGG + chr1_2:27471134-27471153 Msa0065390:intron 25.0%
! TTACATGTGAGTTTATACTA+TGG - chr1_2:27471572-27471591 None:intergenic 25.0%
!!! TGATGATGGTTAAAATTAAC+AGG - chr1_2:27471755-27471774 None:intergenic 25.0%
! GTCACAATAATGAGTTATTT+GGG - chr1_2:27471807-27471826 None:intergenic 25.0%
! AGTCACAATAATGAGTTATT+TGG - chr1_2:27471808-27471827 None:intergenic 25.0%
! GAAAATAATGGCAGAAAATT+TGG - chr1_2:27472089-27472108 None:intergenic 25.0%
! ATCATACAATACAAGTTTGA+GGG - chr1_2:27472121-27472140 None:intergenic 25.0%
! TATCATACAATACAAGTTTG+AGG - chr1_2:27472122-27472141 None:intergenic 25.0%
! CAAACTTGTATTGTATGATA+TGG + chr1_2:27472122-27472141 Msa0065390:intron 25.0%
!! AATGATTTGTATATGTCCTT+AGG + chr1_2:27472188-27472207 Msa0065390:intron 25.0%
! TCAGCATATCTTATGAAAAT+GGG - chr1_2:27473154-27473173 None:intergenic 25.0%
!! TTGATATTTGTCATTTCACT+AGG - chr1_2:27473326-27473345 None:intergenic 25.0%
! ATGACAAATATCAACATGAT+AGG + chr1_2:27473332-27473351 Msa0065390:three_prime_UTR 25.0%
ATAAGTAAGATGTGATGAGA+GGG - chr1_2:27470606-27470625 None:intergenic 30.0%
AATAAGTAAGATGTGATGAG+AGG - chr1_2:27470607-27470626 None:intergenic 30.0%
! TGTATCTAATGAGGAGTTTT+TGG - chr1_2:27470748-27470767 None:intergenic 30.0%
AGATCAAAGATACAAACATG+CGG - chr1_2:27470779-27470798 None:intergenic 30.0%
!!! ACTAGGACTAAGCTTTATTT+TGG + chr1_2:27471011-27471030 Msa0065390:CDS 30.0%
ATTGTGACTTAAACTGTGAA+AGG + chr1_2:27471819-27471838 Msa0065390:intron 30.0%
TTGTGACTTAAACTGTGAAA+GGG + chr1_2:27471820-27471839 Msa0065390:intron 30.0%
ACTTAGTTATGGATAGACTT+TGG + chr1_2:27472024-27472043 Msa0065390:intron 30.0%
GGGATGTATACTGAAAATAA+TGG - chr1_2:27472101-27472120 None:intergenic 30.0%
TTAACATTACGGTAACTCAA+GGG - chr1_2:27472170-27472189 None:intergenic 30.0%
ATTAACATTACGGTAACTCA+AGG - chr1_2:27472171-27472190 None:intergenic 30.0%
GCAGTAATTCAAAATCTTGA+CGG + chr1_2:27472285-27472304 Msa0065390:CDS 30.0%
CAGTAATTCAAAATCTTGAC+GGG + chr1_2:27472286-27472305 Msa0065390:CDS 30.0%
TTGTAAATGAACAACTTCTC+AGG + chr1_2:27472374-27472393 Msa0065390:intron 30.0%
!!! TACAATGGTTCTTTTGGTTT+AGG - chr1_2:27472441-27472460 None:intergenic 30.0%
CACATTATCCATGATTGTAA+GGG - chr1_2:27472649-27472668 None:intergenic 30.0%
GCAATTATATGCAAATGTCA+AGG + chr1_2:27472817-27472836 Msa0065390:CDS 30.0%
TCATAAGGTCGATATAAACT+CGG + chr1_2:27472995-27473014 Msa0065390:exon 30.0%
GTCAGCATATCTTATGAAAA+TGG - chr1_2:27473155-27473174 None:intergenic 30.0%
GATGGAAGATGAAAACAATG+AGG - chr1_2:27470703-27470722 None:intergenic 35.0%
! TAGACATTTGACAGTGTTGA+TGG - chr1_2:27470721-27470740 None:intergenic 35.0%
GTTTGAGGATGTATCTAATG+AGG - chr1_2:27470757-27470776 None:intergenic 35.0%
CAACAGTTTCACACAAGTTA+TGG + chr1_2:27470832-27470851 Msa0065390:CDS 35.0%
AGAAGAAGTAGTAGAAGACA+AGG + chr1_2:27470953-27470972 Msa0065390:CDS 35.0%
GTAGAAGACAAGGAGAATAT+TGG + chr1_2:27470963-27470982 Msa0065390:CDS 35.0%
AAGACAAGGAGAATATTGGA+GGG + chr1_2:27470967-27470986 Msa0065390:CDS 35.0%
AGTGCACTATCAACACTATA+AGG - chr1_2:27471044-27471063 None:intergenic 35.0%
! TGGTAGTGCAGAATTAATTG+AGG + chr1_2:27471088-27471107 Msa0065390:CDS 35.0%
! GCGTATTTAATCTGAAGTGT+CGG - chr1_2:27471178-27471197 None:intergenic 35.0%
TTCAGATTAAATACGCGTCT+AGG + chr1_2:27471182-27471201 Msa0065390:intron 35.0%
TCAGATTAAATACGCGTCTA+GGG + chr1_2:27471183-27471202 Msa0065390:intron 35.0%
!! GAAGATTGTTTGCTATAGCT+AGG + chr1_2:27471330-27471349 Msa0065390:intron 35.0%
!! TTGTTTGCTATAGCTAGGAT+AGG + chr1_2:27471335-27471354 Msa0065390:intron 35.0%
! TCTCGTCTCTAGTTTGATTT+CGG + chr1_2:27471682-27471701 Msa0065390:intron 35.0%
! GATACAGCTGATAATGATGA+TGG - chr1_2:27471769-27471788 None:intergenic 35.0%
TGTGACTTAAACTGTGAAAG+GGG + chr1_2:27471821-27471840 Msa0065390:intron 35.0%
CAAGGGATGAAACATAAACA+TGG - chr1_2:27472153-27472172 None:intergenic 35.0%
TCTCTGTCATAAAGAACCTA+AGG - chr1_2:27472207-27472226 None:intergenic 35.0%
! TCAGGATACAATGGTTCTTT+TGG - chr1_2:27472447-27472466 None:intergenic 35.0%
TACTCAGTTTCAGGATACAA+TGG - chr1_2:27472456-27472475 None:intergenic 35.0%
AAAAGCTTGTACTCAGTTTC+AGG - chr1_2:27472465-27472484 None:intergenic 35.0%
! ACTGAGTACAAGCTTTTTGT+TGG + chr1_2:27472468-27472487 Msa0065390:CDS 35.0%
! CTGAGTACAAGCTTTTTGTT+GGG + chr1_2:27472469-27472488 Msa0065390:CDS 35.0%
AACAGTTCCATGTTCTTGAA+AGG - chr1_2:27472535-27472554 None:intergenic 35.0%
! CATTTCTGCTTTATTGGCAT+AGG - chr1_2:27472619-27472638 None:intergenic 35.0%
CTATGCCAATAAAGCAGAAA+TGG + chr1_2:27472617-27472636 Msa0065390:CDS 35.0%
ATTATCCATGATTGTAAGGG+CGG - chr1_2:27472646-27472665 None:intergenic 35.0%
CCACATTATCCATGATTGTA+AGG - chr1_2:27472650-27472669 None:intergenic 35.0%
CCTTACAATCATGGATAATG+TGG + chr1_2:27472647-27472666 Msa0065390:CDS 35.0%
TTCGCTTAAATTCAAGTCAC+GGG - chr1_2:27472727-27472746 None:intergenic 35.0%
GTTCGCTTAAATTCAAGTCA+CGG - chr1_2:27472728-27472747 None:intergenic 35.0%
TACGTTTGTGTGTGTAAACA+AGG + chr1_2:27472880-27472899 Msa0065390:intron 35.0%
! TCTTGTAATTTGTGCACTTC+AGG + chr1_2:27472925-27472944 Msa0065390:intron 35.0%
TGCAAAGGAACTTCTTATCA+AGG - chr1_2:27473102-27473121 None:intergenic 35.0%
GTTCCTTTGCATATTTCTTG+TGG + chr1_2:27473111-27473130 Msa0065390:three_prime_UTR 35.0%
ACACCACAAGAAATATGCAA+AGG - chr1_2:27473117-27473136 None:intergenic 35.0%
!!! GTTTTAGCTAGTGCTAACAT+TGG + chr1_2:27473200-27473219 Msa0065390:three_prime_UTR 35.0%
AGATACAAACATGCGGTTTG+AGG - chr1_2:27470772-27470791 None:intergenic 40.0%
TTTGATCTCACAATCCTCAG+TGG + chr1_2:27470791-27470810 Msa0065390:CDS 40.0%
GCAACAGCAACAGAAATTCT+AGG - chr1_2:27470861-27470880 None:intergenic 40.0%
! AATTTCTGTTGCTGTTGCAC+AGG + chr1_2:27470863-27470882 Msa0065390:CDS 40.0%
GGATGAAGAGAAAGTTGTAG+TGG + chr1_2:27470908-27470927 Msa0065390:CDS 40.0%
! AGAGAAAGTTGTAGTGGAAG+AGG + chr1_2:27470914-27470933 Msa0065390:CDS 40.0%
GAAGACAAGGAGAATATTGG+AGG + chr1_2:27470966-27470985 Msa0065390:CDS 40.0%
AGACAAGGAGAATATTGGAG+GGG + chr1_2:27470968-27470987 Msa0065390:CDS 40.0%
!! TTGATAGTGCACTACTTGCA+GGG + chr1_2:27471051-27471070 Msa0065390:CDS 40.0%
ACTACTTGCAGGGTTAATTG+AGG + chr1_2:27471061-27471080 Msa0065390:CDS 40.0%
CAGATTAAATACGCGTCTAG+GGG + chr1_2:27471184-27471203 Msa0065390:intron 40.0%
AGATTAAATACGCGTCTAGG+GGG + chr1_2:27471185-27471204 Msa0065390:intron 40.0%
AAATGAAAGCTGCAGCTAGA+AGG - chr1_2:27471466-27471485 None:intergenic 40.0%
GTTCTTTATGACAGAGACAC+TGG + chr1_2:27472210-27472229 Msa0065390:CDS 40.0%
AGAGACACTGGAAAAAGTAG+AGG + chr1_2:27472222-27472241 Msa0065390:CDS 40.0%
AACAACTTCTCAGGAGTTCA+TGG + chr1_2:27472383-27472402 Msa0065390:intron 40.0%
ACAACTTCTCAGGAGTTCAT+GGG + chr1_2:27472384-27472403 Msa0065390:intron 40.0%
! CAAGCTTTTTGTTGGGAACT+TGG + chr1_2:27472476-27472495 Msa0065390:CDS 40.0%
! TTTTTGTTGGGAACTTGGCT+TGG + chr1_2:27472481-27472500 Msa0065390:CDS 40.0%
ACACAAGCCTTTCAAGAACA+TGG + chr1_2:27472525-27472544 Msa0065390:CDS 40.0%
CAAGAACATGGAACTGTTGT+TGG + chr1_2:27472537-27472556 Msa0065390:CDS 40.0%
! GTGTTATATGATGGCGAAAC+CGG + chr1_2:27472567-27472586 Msa0065390:CDS 40.0%
GGCATCCATTTCTGCTTTAT+TGG - chr1_2:27472625-27472644 None:intergenic 40.0%
!! GCACAGCGACTTTTAGAAAA+CGG + chr1_2:27472701-27472720 Msa0065390:intron 40.0%
GCTCAAGGAAAACGTTCATA+AGG + chr1_2:27472980-27472999 Msa0065390:CDS 40.0%
AGGTCGATATAAACTCGGAA+AGG + chr1_2:27473000-27473019 Msa0065390:exon 40.0%
GGCTCATTGATGTTCAACAA+TGG + chr1_2:27473021-27473040 Msa0065390:three_prime_UTR 40.0%
! ATGGTGCCAAAGAAATGAGA+AGG + chr1_2:27473040-27473059 Msa0065390:three_prime_UTR 40.0%
! TGGTGCCAAAGAAATGAGAA+GGG + chr1_2:27473041-27473060 Msa0065390:three_prime_UTR 40.0%
CCATGTTGTTGTGATGACAG+TGG - chr1_2:27470662-27470681 None:intergenic 45.0%
CCACTGTCATCACAACAACA+TGG + chr1_2:27470659-27470678 Msa0065390:exon 45.0%
AATGGACAAAGGTTCCACTG+AGG - chr1_2:27470808-27470827 None:intergenic 45.0%
TCAGTGGAACCTTTGTCCAT+TGG + chr1_2:27470807-27470826 Msa0065390:CDS 45.0%
ACTGTTGTGCCAATGGACAA+AGG - chr1_2:27470819-27470838 None:intergenic 45.0%
GTGTGAAACTGTTGTGCCAA+TGG - chr1_2:27470826-27470845 None:intergenic 45.0%
CTGTAGCAGAAGTAGACACT+AGG + chr1_2:27470994-27471013 Msa0065390:CDS 45.0%
!! GTTGATAGTGCACTACTTGC+AGG + chr1_2:27471050-27471069 Msa0065390:CDS 45.0%
GCAGGGTTAATTGAGGAGTA+TGG + chr1_2:27471068-27471087 Msa0065390:CDS 45.0%
CTAGGGGGTTCAACATATGT+TGG + chr1_2:27471200-27471219 Msa0065390:intron 45.0%
!! ACATATGTTGGTGTCGCAAG+TGG + chr1_2:27471212-27471231 Msa0065390:intron 45.0%
CAACTTCTCAGGAGTTCATG+GGG + chr1_2:27472385-27472404 Msa0065390:intron 45.0%
ATGGAACTGTTGTTGGAGCT+AGG + chr1_2:27472544-27472563 Msa0065390:CDS 45.0%
TGGAACTGTTGTTGGAGCTA+GGG + chr1_2:27472545-27472564 Msa0065390:CDS 45.0%
! GGAGCTAGGGTGTTATATGA+TGG + chr1_2:27472558-27472577 Msa0065390:CDS 45.0%
TGTGCACTTCAGGAACTAGA+AGG + chr1_2:27472935-27472954 Msa0065390:intron 45.0%
ACGAACATTGCGAGTAAGCT+TGG + chr1_2:27472958-27472977 Msa0065390:CDS 45.0%
GGTGCCAAAGAAATGAGAAG+GGG + chr1_2:27473042-27473061 Msa0065390:three_prime_UTR 45.0%
CCATCCCCTTCTCATTTCTT+TGG - chr1_2:27473049-27473068 None:intergenic 45.0%
CCAAAGAAATGAGAAGGGGA+TGG + chr1_2:27473046-27473065 Msa0065390:three_prime_UTR 45.0%
AGTGCATCATGGTAGGTACA+TGG - chr1_2:27473072-27473091 None:intergenic 45.0%
TGAAGCAAGTGCATCATGGT+AGG - chr1_2:27473079-27473098 None:intergenic 45.0%
AAGGTGAAGCAAGTGCATCA+TGG - chr1_2:27473083-27473102 None:intergenic 45.0%
!!! ATAATTTTTTAAAATGAAAT+CGG - chr1_2:27471246-27471265 None:intergenic 5.0%
AACAACATGGCTACTGCAGC+AGG + chr1_2:27470672-27470691 Msa0065390:exon 50.0%
! TGTTGCTGTTGCACAGGAAG+AGG + chr1_2:27470869-27470888 Msa0065390:CDS 50.0%
TTGCGAGTAAGCTTGGCTCA+AGG + chr1_2:27472965-27472984 Msa0065390:CDS 50.0%
TGCACAGGAAGAGGCTGTTG+CGG + chr1_2:27470878-27470897 Msa0065390:CDS 55.0%
AGTTCATGGGGCGAACCTTG+CGG + chr1_2:27472397-27472416 Msa0065390:CDS 55.0%
GTCAGCGAAATTCACCCGCA+AGG - chr1_2:27472415-27472434 None:intergenic 55.0%
ACCGGAAAGTCTCGTGGCTA+TGG + chr1_2:27472585-27472604 Msa0065390:CDS 55.0%
GCCATAGCCACGAGACTTTC+CGG - chr1_2:27472589-27472608 None:intergenic 55.0%
GGATGCCGCCCTTACAATCA+TGG + chr1_2:27472638-27472657 Msa0065390:CDS 55.0%
CATGGCTACTGCAGCAGGCA+TGG + chr1_2:27470677-27470696 Msa0065390:exon 60.0%
AGAGGCTGTTGCGGCTGTTG+AGG + chr1_2:27470887-27470906 Msa0065390:CDS 60.0%
GTTCATGGGGCGAACCTTGC+GGG + chr1_2:27472398-27472417 Msa0065390:CDS 60.0%
GGCGAAACCGGAAAGTCTCG+TGG + chr1_2:27472579-27472598 Msa0065390:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 27470595 27473363 27470595 ID=Msa0065390;Name=Msa0065390
chr1_2 mRNA 27470595 27473363 27470595 ID=Msa0065390-mRNA-1;Parent=Msa0065390;Name=Msa0065390-mRNA-1;_AED=0.06;_eAED=0.06;_QI=83|0.5|0.8|0.8|1|1|5|363|310
chr1_2 exon 27470595 27471109 27470595 ID=Msa0065390-mRNA-1:exon:9546;Parent=Msa0065390-mRNA-1
chr1_2 exon 27472210 27472311 27472210 ID=Msa0065390-mRNA-1:exon:9547;Parent=Msa0065390-mRNA-1
chr1_2 exon 27472396 27472668 27472396 ID=Msa0065390-mRNA-1:exon:9548;Parent=Msa0065390-mRNA-1
chr1_2 exon 27472774 27472845 27472774 ID=Msa0065390-mRNA-1:exon:9549;Parent=Msa0065390-mRNA-1
chr1_2 exon 27472947 27473363 27472947 ID=Msa0065390-mRNA-1:exon:9550;Parent=Msa0065390-mRNA-1
chr1_2 five_prime_UTR 27470595 27470677 27470595 ID=Msa0065390-mRNA-1:five_prime_utr;Parent=Msa0065390-mRNA-1
chr1_2 CDS 27470678 27471109 27470678 ID=Msa0065390-mRNA-1:cds;Parent=Msa0065390-mRNA-1
chr1_2 CDS 27472210 27472311 27472210 ID=Msa0065390-mRNA-1:cds;Parent=Msa0065390-mRNA-1
chr1_2 CDS 27472396 27472668 27472396 ID=Msa0065390-mRNA-1:cds;Parent=Msa0065390-mRNA-1
chr1_2 CDS 27472774 27472845 27472774 ID=Msa0065390-mRNA-1:cds;Parent=Msa0065390-mRNA-1
chr1_2 CDS 27472947 27473000 27472947 ID=Msa0065390-mRNA-1:cds;Parent=Msa0065390-mRNA-1
chr1_2 three_prime_UTR 27473001 27473363 27473001 ID=Msa0065390-mRNA-1:three_prime_utr;Parent=Msa0065390-mRNA-1
Gene Sequence

>Msa0065390

ATGGCTACTGCAGCAGGCATGGCCTCATTGTTTTCATCTTCCATCAACACTGTCAAATGTCTACGTTCCAAAAACTCCTCATTAGATACATCCTCAAACCGCATGTTTGTATCTTTGATCTCACAATCCTCAGTGGAACCTTTGTCCATTGGCACAACAGTTTCACACAAGTTATGGTTTCCTAGAATTTCTGTTGCTGTTGCACAGGAAGAGGCTGTTGCGGCTGTTGAGGATGAAGAGAAAGTTGTAGTGGAAGAGGAAAAAAAAGAAGAAGAAGAAGAAGTAGTAGAAGACAAGGAGAATATTGGAGGGGAAACTGTAGCAGAAGTAGACACTAGGACTAAGCTTTATTTTGGCAATTTGCCTTATAGTGTTGATAGTGCACTACTTGCAGGGTTAATTGAGGAGTATGGTAGTGCAGAATTAATTGAGGTTCTTTATGACAGAGACACTGGAAAAAGTAGAGGCTTTGCATTTGTGACAATGAGTTGTGTTGAAGATTGTAATGCAGTAATTCAAAATCTTGACGGGAAAGAGTTCATGGGGCGAACCTTGCGGGTGAATTTCGCTGACAAACCTAAACCAAAAGAACCATTGTATCCTGAAACTGAGTACAAGCTTTTTGTTGGGAACTTGGCTTGGACAGTAACTTCTGAGAGTCTTACACAAGCCTTTCAAGAACATGGAACTGTTGTTGGAGCTAGGGTGTTATATGATGGCGAAACCGGAAAGTCTCGTGGCTATGGCTTTGTTTCCTATGCCAATAAAGCAGAAATGGATGCCGCCCTTACAATCATGGATAATGTGCATAATGTGACAACAATTGACGCTGAATCAGCAGAATTTGACTGCAATTATATGCAAATGTCAAGGATAGCTGAACTAGAAGGACGAACATTGCGAGTAAGCTTGGCTCAAGGAAAACGTTCATAA
Protein sequence

>Msa0065390

MATAAGMASLFSSSINTVKCLRSKNSSLDTSSNRMFVSLISQSSVEPLSIGTTVSHKLWFPRISVAVAQEEAVAAVEDEEKVVVEEEKKEEEEEVVEDKENIGGETVAEVDTRTKLYFGNLPYSVDSALLAGLIEEYGSAELIEVLYDRDTGKSRGFAFVTMSCVEDCNAVIQNLDGKEFMGRTLRVNFADKPKPKEPLYPETEYKLFVGNLAWTVTSESLTQAFQEHGTVVGARVLYDGETGKSRGYGFVSYANKAEMDAALTIMDNVHNVTTIDAESAEFDCNYMQMSRIAELEGRTLRVSLAQGKRS*