AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0988030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0988030 MtrunA17_Chr6g0467861 97.041 507 15 0 1 507 1 507 0.0 1012
Msa0988030 MtrunA17_Chr5g0436621 31.384 513 257 11 49 504 8 482 2.43e-76 248
Msa0988030 MtrunA17_Chr4g0005941 33.266 493 291 10 44 507 3 486 2.23e-75 246
Msa0988030 MtrunA17_Chr3g0113181 30.584 497 274 8 42 504 7 466 6.31e-71 233
Msa0988030 MtrunA17_Chr3g0134041 29.857 489 285 11 34 507 2 447 1.05e-63 214
Msa0988030 MtrunA17_Chr3g0134121 32.495 477 270 12 46 504 5 447 1.70e-63 214
Msa0988030 MtrunA17_Chr3g0134111 31.224 474 283 11 47 507 7 450 9.56e-61 206
Msa0988030 MtrunA17_Chr3g0134061 29.412 493 284 10 44 507 2 459 5.73e-60 204
Msa0988030 MtrunA17_Chr4g0053111 31.590 478 272 12 46 504 3 444 6.58e-60 204
Msa0988030 MtrunA17_Chr4g0010381 30.435 483 279 13 30 504 3 436 2.62e-59 202
Msa0988030 MtrunA17_Chr3g0134091 30.737 475 278 11 46 503 5 445 3.29e-59 202
Msa0988030 MtrunA17_Chr8g0359251 30.425 447 266 9 67 504 28 438 1.73e-56 194
Msa0988030 MtrunA17_Chr4g0010361 30.621 467 283 12 47 504 4 438 9.36e-56 192
Msa0988030 MtrunA17_Chr3g0134071 28.831 496 282 12 34 504 2 451 2.50e-53 187
Msa0988030 MtrunA17_Chr8g0354371 31.166 446 260 11 68 504 30 437 6.65e-53 185
Msa0988030 MtrunA17_Chr4g0010311 29.185 466 290 12 47 504 4 437 9.88e-52 182
Msa0988030 MtrunA17_Chr3g0134101 30.417 480 266 13 46 507 22 451 1.03e-51 182
Msa0988030 MtrunA17_Chr3g0125001 30.735 449 264 11 64 504 20 429 3.02e-51 180
Msa0988030 MtrunA17_Chr4g0053081 29.688 448 269 11 68 505 32 443 8.17e-50 177
Msa0988030 MtrunA17_Chr4g0010331 29.810 473 272 14 47 504 4 431 1.26e-49 176
Msa0988030 MtrunA17_Chr3g0087221 29.915 468 291 12 47 504 4 444 3.01e-49 175
Msa0988030 MtrunA17_Chr7g0224131 29.237 472 279 13 47 504 4 434 2.30e-48 172
Msa0988030 MtrunA17_Chr8g0374911 30.905 453 267 13 58 495 40 461 6.55e-46 167
Msa0988030 MtrunA17_Chr4g0053061 31.151 443 259 13 68 495 57 468 1.56e-44 163
Msa0988030 MtrunA17_Chr4g0053091 29.167 456 253 13 67 505 30 432 2.44e-44 162
Msa0988030 MtrunA17_Chr8g0371821 27.197 478 280 21 47 507 3 429 1.73e-40 151
Msa0988030 MtrunA17_Chr4g0001861 26.349 482 283 18 47 507 3 433 1.65e-39 148
Msa0988030 MtrunA17_Chr4g0010121 28.409 440 262 10 64 489 65 465 1.17e-38 147
Msa0988030 MtrunA17_Chr8g0374641 29.741 464 264 18 47 489 4 426 6.29e-38 144
Msa0988030 MtrunA17_Chr4g0010351 28.040 403 240 10 46 434 3 369 1.88e-37 141
Msa0988030 MtrunA17_Chr4g0054081 28.329 413 239 10 99 490 65 441 3.92e-35 137
Msa0988030 MtrunA17_Chr2g0334001 24.520 469 294 16 47 499 3 427 2.27e-32 128
Msa0988030 MtrunA17_Chr4g0072041 26.859 417 247 12 99 494 70 449 1.96e-29 120
Msa0988030 MtrunA17_Chr3g0087401 26.835 436 259 15 47 456 2 403 2.69e-29 120
Msa0988030 MtrunA17_Chr8g0386141 27.158 475 285 18 45 506 5 431 2.69e-27 114
Msa0988030 MtrunA17_Chr3g0132001 27.311 476 290 17 34 499 1 430 1.87e-26 111
Msa0988030 MtrunA17_Chr2g0311401 24.096 332 195 10 179 494 2 292 7.35e-25 104
Msa0988030 MtrunA17_Chr8g0353901 30.374 214 141 5 296 505 20 229 2.33e-24 102
Msa0988030 MtrunA17_Chr6g0455341 27.494 451 268 19 68 494 34 449 3.78e-24 105
Msa0988030 MtrunA17_Chr7g0219121 24.696 494 295 20 46 495 7 467 4.05e-23 102
Msa0988030 MtrunA17_Chr7g0219291 26.742 445 266 17 46 459 355 770 1.29e-22 102
Msa0988030 MtrunA17_Chr7g0253541 24.532 481 306 17 45 499 2 451 3.81e-22 99.4
Msa0988030 MtrunA17_Chr6g0456591 32.461 191 124 4 47 233 18 207 2.43e-21 92.8
Msa0988030 MtrunA17_Chr7g0224121 36.054 147 77 3 81 217 39 178 6.32e-21 90.9
Msa0988030 MtrunA17_Chr7g0224391 26.392 485 279 21 43 507 11 437 1.08e-20 94.7
Msa0988030 MtrunA17_Chr6g0455361 26.058 449 274 20 68 494 34 446 1.67e-20 94.4
Msa0988030 MtrunA17_Chr2g0323511 25.052 483 308 17 39 490 8 467 3.75e-19 90.1
Msa0988030 MtrunA17_Chr6g0463321 29.282 181 93 4 296 475 38 184 5.79e-19 84.7
Msa0988030 MtrunA17_Chr4g0053071 39.310 145 80 5 365 505 3 143 1.23e-18 82.8
Msa0988030 MtrunA17_Chr6g0463331 40.870 115 62 2 110 218 45 159 3.54e-18 82.0
Msa0988030 MtrunA17_Chr6g0454761 25.910 467 296 20 47 494 8 443 3.85e-18 88.2
Msa0988030 MtrunA17_Chr7g0219181 24.949 489 296 19 46 494 7 464 1.12e-17 85.5
Msa0988030 MtrunA17_Chr4g0053051 41.284 109 62 2 400 507 23 130 9.22e-17 77.0
Msa0988030 MtrunA17_Chr8g0353891 32.948 173 109 4 67 232 26 198 1.09e-16 79.3
Msa0988030 MtrunA17_Chr3g0134131 34.507 142 87 3 317 455 143 281 1.21e-16 80.9
Msa0988030 MtrunA17_Chr2g0306321 24.330 448 271 23 77 499 51 455 1.45e-15 79.0
Msa0988030 MtrunA17_Chr3g0087901 24.105 419 257 16 68 457 31 417 2.97e-15 78.2
Msa0988030 MtrunA17_Chr7g0252731 25.501 349 220 12 35 376 5 320 1.43e-14 75.9
Msa0988030 MtrunA17_Chr6g0455351 26.524 328 196 13 68 376 34 335 2.10e-14 75.1
Msa0988030 MtrunA17_Chr1g0165661 23.958 384 215 14 93 455 58 385 4.90e-14 74.3
Msa0988030 MtrunA17_Chr3g0097841 24.880 209 139 4 297 490 26 231 1.00e-12 68.2
Msa0988030 MtrunA17_Chr7g0224101 30.833 120 80 1 269 388 16 132 2.15e-12 64.7
Msa0988030 MtrunA17_Chr4g0004701 28.646 192 107 6 325 494 48 231 5.21e-12 65.9
Msa0988030 MtrunA17_Chr2g0283361 24.595 370 229 14 99 459 58 386 4.77e-11 64.7
Msa0988030 MtrunA17_Chr7g0219211 25.359 209 120 5 46 232 8 202 6.96e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0988030 AT5G23940.1 62.447 474 140 6 45 505 1 449 0.0 596
Msa0988030 AT2G39980.1 31.176 510 266 9 46 507 7 479 9.23e-75 244
Msa0988030 AT5G01210.1 32.406 503 264 7 44 507 3 468 5.53e-74 242
Msa0988030 AT5G42830.1 33.843 458 260 13 57 504 21 445 6.45e-66 220
Msa0988030 AT5G07870.1 31.092 476 284 9 47 504 8 457 8.80e-60 204
Msa0988030 AT5G07860.1 30.769 481 267 12 47 504 8 445 9.15e-60 204
Msa0988030 AT5G67150.1 31.078 473 282 11 47 507 5 445 9.36e-59 201
Msa0988030 AT3G50280.1 30.290 482 268 16 46 504 3 439 5.18e-57 196
Msa0988030 AT3G50270.1 29.193 483 279 12 47 507 6 447 8.40e-57 196
Msa0988030 AT5G07850.1 31.315 479 277 17 47 507 8 452 1.81e-55 192
Msa0988030 AT3G50300.1 28.750 480 288 12 46 507 3 446 1.40e-50 179
Msa0988030 AT5G67160.1 29.873 472 263 17 49 503 7 427 5.33e-45 164
Msa0988030 AT3G50280.2 31.054 351 186 11 167 504 2 309 1.65e-43 156
Msa0988030 AT5G38130.1 26.782 463 287 11 41 489 18 442 7.59e-42 155
Msa0988030 AT5G48930.1 25.424 472 304 17 45 507 1 433 1.43e-33 132
Msa0988030 AT2G19070.1 27.484 473 273 16 52 494 8 440 3.74e-32 128
Msa0988030 AT3G50290.1 27.687 307 172 7 160 454 1 269 1.00e-28 115
Msa0988030 AT5G41040.1 26.572 493 303 15 15 494 1 447 2.19e-27 115
Msa0988030 AT5G41040.2 27.214 463 278 15 45 494 15 431 1.07e-26 112
Msa0988030 AT5G63560.1 27.404 416 235 14 93 494 55 417 6.96e-25 107
Msa0988030 AT5G57840.1 26.068 468 275 18 45 487 1 422 1.26e-23 103
Msa0988030 AT3G48720.1 26.540 422 245 16 93 499 55 426 6.29e-23 101
Msa0988030 AT3G29635.1 27.791 421 254 17 68 467 29 420 5.60e-22 99.0
Msa0988030 AT1G03940.1 25.820 488 278 23 48 494 9 453 2.23e-20 94.4
Msa0988030 AT5G39080.1 25.376 465 273 16 68 497 28 453 1.04e-18 89.4
Msa0988030 AT5G61160.1 24.060 399 266 12 71 456 28 402 3.24e-17 84.3
Msa0988030 AT5G07080.1 22.944 462 319 11 34 487 1 433 5.78e-16 80.5
Msa0988030 AT1G03495.1 27.429 350 190 18 57 376 19 334 1.07e-15 79.0
Msa0988030 AT1G32910.1 22.396 384 252 10 84 456 50 398 1.94e-15 79.0
Msa0988030 AT3G29680.1 26.139 417 263 15 68 467 25 413 2.05e-15 79.0
Msa0988030 AT5G39090.1 25.277 451 277 18 68 497 28 439 4.95e-15 77.8
Msa0988030 AT1G03390.1 24.747 396 241 18 77 456 52 406 1.25e-14 76.6
Msa0988030 AT5G39050.1 24.926 337 202 9 151 459 109 422 8.23e-14 73.9
Msa0988030 AT3G47170.1 24.633 341 209 14 102 425 73 382 4.42e-13 71.6
Msa0988030 AT1G28680.1 24.172 302 189 9 173 470 135 400 2.63e-12 69.3
Msa0988030 AT1G28680.2 25.108 231 141 6 173 402 135 334 3.71e-12 68.6
Msa0988030 AT2G23510.1 23.095 420 262 20 55 456 21 397 3.67e-11 65.9

Find 150 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGTGCAATAAGAGAATT+TGG 0.179714 6_4:+40283164 Msa0988030:three_prime_UTR
TGGAAGTTCACCTAGGTTTA+AGG 0.194532 6_4:+40282938 Msa0988030:CDS
CACACGTGTGTTTCGGGCCT+TGG 0.203708 6_4:-40282322 None:intergenic
GGTTGATGGTGGTGGGCCTT+TGG 0.220575 6_4:-40282370 None:intergenic
TCACCATTGGCTGATGGTGC+TGG 0.226535 6_4:-40282423 None:intergenic
TTATAGTGGAATCTTGAATT+TGG 0.232801 6_4:+40279792 Msa0988030:CDS
GTGTTGCTGGCATGCATTTA+TGG 0.233290 6_4:-40279288 None:intergenic
CATCAACTAGCTGGTAAATT+AGG 0.237092 6_4:+40279628 Msa0988030:CDS
AGGCTCTCTCCACTCATATT+TGG 0.242867 6_4:+40282610 Msa0988030:CDS
CATGGCCTTCAATTACCTTT+TGG 0.245260 6_4:-40282824 None:intergenic
GTTGATGGTGGTGGGCCTTT+GGG 0.256743 6_4:-40282369 None:intergenic
CGTTACGGCTGCTGGGCTTT+TGG 0.257725 6_4:+40282767 Msa0988030:CDS
AGTTAATATTATCTTAATAA+TGG 0.257779 6_4:+40283369 Msa0988030:three_prime_UTR
TAGACCCTTGTTGGCTGTTC+AGG 0.257806 6_4:+40279825 Msa0988030:CDS
TCTCTTATTGCACAAATTCT+TGG 0.259588 6_4:-40283158 None:intergenic
AAGGTTTATGACATTGATTT+TGG 0.269945 6_4:+40282957 Msa0988030:CDS
GCAAGATAAGGAATTTCTTA+TGG 0.279006 6_4:+40283118 Msa0988030:CDS
AAAATCATTCTCAACTTTCC+AGG 0.285870 6_4:+40282590 Msa0988030:CDS
TGGTGGTGGGCCTTTGGGCT+CGG 0.285991 6_4:-40282364 None:intergenic
CAAGCTTCACACGTGTGTTT+CGG 0.288866 6_4:-40282329 None:intergenic
TTGAACTTGGTAATAATGTT+TGG 0.293294 6_4:+40283325 Msa0988030:three_prime_UTR
CACAACAGCCCAACCATTCT+TGG 0.294816 6_4:+40282293 Msa0988030:CDS
AGGAATCAATTCCTTCAAAA+TGG 0.325774 6_4:-40279770 None:intergenic
AAGCTTCACACGTGTGTTTC+GGG 0.326495 6_4:-40282328 None:intergenic
CGGTGAATATAGCTTGAATT+AGG 0.326763 6_4:-40282746 None:intergenic
TTTAACCATGCTGTCCTTGA+TGG 0.331947 6_4:+40282213 Msa0988030:CDS
TGAACTTGGTAATAATGTTT+GGG 0.347157 6_4:+40283326 Msa0988030:three_prime_UTR
GCATGCATTTATGGAATTCA+TGG 0.349511 6_4:-40279279 None:intergenic
CATCCCATCAAATTTGTTGT+TGG 0.353936 6_4:-40283012 None:intergenic
AAATTCAAGATTCCACTATA+AGG 0.356072 6_4:-40279790 None:intergenic
ATATTCACCGTTACGGCTGC+TGG 0.368878 6_4:+40282759 Msa0988030:CDS
GCCTTGGTTCTTTCCAAGAA+TGG 0.370836 6_4:-40282306 None:intergenic
AAGGAATTGATTCCTTATAG+TGG 0.380758 6_4:+40279778 Msa0988030:CDS
TTTACCTCACCATTGGTTGA+TGG 0.382680 6_4:-40282384 None:intergenic
GTGAACACTGTGTAGTCTTC+GGG 0.385741 6_4:-40282663 None:intergenic
GGTTCTTTCCAAGAATGGTT+GGG 0.392557 6_4:-40282301 None:intergenic
AACATTGGAGCCTGAGGCTA+TGG 0.403215 6_4:+40283085 Msa0988030:CDS
CACCATTGGCTGATGGTGCT+GGG 0.403622 6_4:-40282422 None:intergenic
GGTGGTGGGCCTTTGGGCTC+GGG 0.405627 6_4:-40282363 None:intergenic
GTTCCACTTCTCACAATCTC+TGG 0.407858 6_4:-40282990 None:intergenic
CGTGGCACTTCATGACTTCA+TGG 0.418816 6_4:+40282244 Msa0988030:CDS
TTCTTATAGAAAAGTGTTGC+TGG 0.419048 6_4:-40279301 None:intergenic
GCGGCTCACCCGCCACAATT+CGG 0.419308 6_4:+40282789 Msa0988030:CDS
AACCGTCCGATGGAAGGGTT+TGG 0.420668 6_4:-40282566 None:intergenic
GTGAGGCAAGGCCCGTACTT+AGG 0.422218 6_4:+40282487 Msa0988030:CDS
TTGATATAATGTTGTGTGTT+GGG 0.425059 6_4:+40283268 Msa0988030:three_prime_UTR
GGAACCAACAACAAATTTGA+TGG 0.429115 6_4:+40283008 Msa0988030:CDS
CATCAATAGCTTTAGCATCA+TGG 0.431488 6_4:-40282842 None:intergenic
TGGTTCTTTCCAAGAATGGT+TGG 0.433192 6_4:-40282302 None:intergenic
CAGCTAACAAAGCTTAAAGA+TGG 0.433635 6_4:+40282171 Msa0988030:intron
CTTTGGGCTCGGGTAGGGAG+AGG 0.433813 6_4:-40282353 None:intergenic
TGGGCCTTTGGGCTCGGGTA+GGG 0.433931 6_4:-40282358 None:intergenic
CTCCTCCCTCCACTCTTGCC+TGG 0.434809 6_4:-40283047 None:intergenic
TTCATCCTTTACCTCACCAT+TGG 0.434849 6_4:-40282391 None:intergenic
TTGATCCAAAAGGTAATTGA+AGG 0.436135 6_4:+40282819 Msa0988030:CDS
CCAAAGTATGCCTCCGGCAT+TGG 0.437756 6_4:-40282720 None:intergenic
TTCTATGTTTAGATTGTTGA+AGG 0.440887 6_4:+40283190 Msa0988030:three_prime_UTR
CGGCGCTTCTTTGATCCAAA+AGG 0.441589 6_4:+40282809 Msa0988030:CDS
TTCAACCTTTGCCTCACCAT+TGG 0.443928 6_4:-40282436 None:intergenic
GGGATGATTTATTTGTATCC+AGG 0.445149 6_4:+40283029 Msa0988030:CDS
TGAGGCAAGGCCCGTACTTA+GGG 0.446867 6_4:+40282488 Msa0988030:CDS
CCAATGCCGGAGGCATACTT+TGG 0.452064 6_4:+40282720 Msa0988030:CDS
TGTGAACACTGTGTAGTCTT+CGG 0.453755 6_4:-40282664 None:intergenic
GTGGGCCTTTGGGCTCGGGT+AGG 0.453995 6_4:-40282359 None:intergenic
CTTGCCTCACCTTTGACTGA+TGG 0.456250 6_4:-40282474 None:intergenic
TCACCATTGGTTGATGGTGG+TGG 0.462091 6_4:-40282378 None:intergenic
AAATATGAGTGGAGAGAGCC+TGG 0.462148 6_4:-40282608 None:intergenic
GCCTCACCTTTGACTGATGG+TGG 0.464493 6_4:-40282471 None:intergenic
CTCACCTTTGACTGATGGTG+GGG 0.466633 6_4:-40282469 None:intergenic
AATGTTGAGAAACTCAAGAA+TGG 0.466672 6_4:+40279580 Msa0988030:CDS
CTTAAAGATGGGCTAGCAAT+GGG 0.473029 6_4:+40282183 Msa0988030:CDS
AAGGAGTTTCATCAACTAGC+TGG 0.476215 6_4:+40279619 Msa0988030:CDS
GAATGGTTGGGCTGTTGTGA+TGG 0.476607 6_4:-40282289 None:intergenic
GAAGCTGTTGCTGATGAGAT+TGG 0.480686 6_4:+40279715 Msa0988030:CDS
CCTCACCTTTGACTGATGGT+GGG 0.484738 6_4:-40282470 None:intergenic
GCCTGAGGCTATGGGAAGGT+TGG 0.493581 6_4:+40283094 Msa0988030:CDS
TCAAGCTATATTCACCGTTA+CGG 0.499928 6_4:+40282752 Msa0988030:CDS
ATCAACCAATGGTGAGGTAA+AGG 0.505373 6_4:+40282386 Msa0988030:CDS
AGATGGGCTAGCAATGGGCT+TGG 0.506336 6_4:+40282188 Msa0988030:CDS
TTTGCCTCACCATTGGCTGA+TGG 0.513475 6_4:-40282429 None:intergenic
TCCAACCTTCCCATAGCCTC+AGG 0.514780 6_4:-40283095 None:intergenic
AAGTATGCCTCCGGCATTGG+TGG 0.514989 6_4:-40282717 None:intergenic
AGTGGAATCTTGAATTTGGA+AGG 0.516771 6_4:+40279796 Msa0988030:CDS
CATTGATGTTGAATTAACAT+TGG 0.517058 6_4:+40283070 Msa0988030:CDS
GCTTTGATTGAATTTGAACT+TGG 0.517937 6_4:+40283312 Msa0988030:three_prime_UTR
AGGTTTCCAAAGTATGCCTC+CGG 0.518580 6_4:-40282726 None:intergenic
GCTTAAAGATGGGCTAGCAA+TGG 0.522409 6_4:+40282182 Msa0988030:CDS
ATGTCATAAACCTTAAACCT+AGG 0.527449 6_4:-40282948 None:intergenic
ACGAGTGAACCCACCAATGC+CGG 0.528510 6_4:+40282707 Msa0988030:CDS
ATGTTGAGAAACTCAAGAAT+GGG 0.530484 6_4:+40279581 Msa0988030:CDS
GGGTCCACCACTGCATATCT+CGG 0.531397 6_4:-40282268 None:intergenic
TTTGATATAATGTTGTGTGT+TGG 0.531835 6_4:+40283267 Msa0988030:three_prime_UTR
TATTCACCGTTACGGCTGCT+GGG 0.540462 6_4:+40282760 Msa0988030:CDS
GGGAAGGTTGGAGCAAGATA+AGG 0.542296 6_4:+40283106 Msa0988030:CDS
CACCATTGGTTGATGGTGGT+GGG 0.542387 6_4:-40282377 None:intergenic
GGTGTGAATTGTGTTGCAGT+TGG 0.547554 6_4:+40282918 Msa0988030:CDS
AAGAAGCGCCGAATTGTGGC+GGG 0.553972 6_4:-40282797 None:intergenic
AGCTAACAAAGCTTAAAGAT+GGG 0.555121 6_4:+40282172 Msa0988030:intron
AATGGTTGGGCTGTTGTGAT+GGG 0.555391 6_4:-40282288 None:intergenic
CAAAAGCCCAGCAGCCGTAA+CGG 0.558033 6_4:-40282766 None:intergenic
ATCAAAGAAGCGCCGAATTG+TGG 0.558770 6_4:-40282801 None:intergenic
TGTATCCAGGCAAGAGTGGA+GGG 0.559507 6_4:+40283042 Msa0988030:CDS
GAATATGATGATGACTTGGT+TGG 0.560722 6_4:+40279679 Msa0988030:CDS
TGTTGAATATGATGATGACT+TGG 0.566299 6_4:+40279675 Msa0988030:CDS
ATTTATGGAATTCATGGAAG+CGG 0.566499 6_4:-40279273 None:intergenic
TCACCTTTGACTGATGGTGG+GGG 0.566819 6_4:-40282468 None:intergenic
AAGCCAGAGATTGTGAGAAG+TGG 0.568369 6_4:+40282987 Msa0988030:CDS
TTGATGAACGTAACAAAGAG+TGG 0.570116 6_4:+40282859 Msa0988030:CDS
GTGGCACTTCATGACTTCAT+GGG 0.571085 6_4:+40282245 Msa0988030:CDS
ATCAGCCAATGGTGAGGCAA+AGG 0.577906 6_4:+40282431 Msa0988030:CDS
TTGTATCCAGGCAAGAGTGG+AGG 0.579701 6_4:+40283041 Msa0988030:CDS
TAAATTAGGTAAAGATGAAG+AGG 0.579860 6_4:+40279642 Msa0988030:CDS
AGTATGCCTCCGGCATTGGT+GGG 0.580863 6_4:-40282716 None:intergenic
AGGCATGCATAGACCCTTGT+TGG 0.581452 6_4:+40279816 Msa0988030:CDS
ACCTCACCATTGGTTGATGG+TGG 0.581928 6_4:-40282381 None:intergenic
GAACCAACAACAAATTTGAT+GGG 0.585695 6_4:+40283009 Msa0988030:CDS
TCTATGTTTAGATTGTTGAA+GGG 0.586256 6_4:+40283191 Msa0988030:three_prime_UTR
AATTACCTGAACAGCCAACA+AGG 0.594893 6_4:-40279830 None:intergenic
AAAGAAGCGCCGAATTGTGG+CGG 0.595569 6_4:-40282798 None:intergenic
TTGCAGTTGGAAGTTCACCT+AGG 0.596541 6_4:+40282931 Msa0988030:CDS
CTCTCCCTACCCGAGCCCAA+AGG 0.598539 6_4:+40282354 Msa0988030:CDS
GTTCACGTGCAAGGCTCACG+TGG 0.602181 6_4:-40282635 None:intergenic
ACATTGGAGCCTGAGGCTAT+GGG 0.602218 6_4:+40283086 Msa0988030:CDS
TGATGAACGTAACAAAGAGT+GGG 0.610084 6_4:+40282860 Msa0988030:CDS
AACCAAACCCTTCCATCGGA+CGG 0.613822 6_4:+40282564 Msa0988030:CDS
AGCACCATCAGCCAATGGTG+AGG 0.618064 6_4:+40282425 Msa0988030:CDS
GCCACGTGGATGTTCCATCA+AGG 0.620237 6_4:-40282227 None:intergenic
TGAATTAACATTGGAGCCTG+AGG 0.623430 6_4:+40283079 Msa0988030:CDS
GAGGTAAAGGATGAACAGAA+TGG 0.627659 6_4:+40282399 Msa0988030:CDS
GGCCCAGCACCATCAGCCAA+TGG 0.630831 6_4:+40282420 Msa0988030:CDS
CATCAGTCAAAGGTGAGGCA+AGG 0.631349 6_4:+40282475 Msa0988030:CDS
CACGTGGCGCCAAATATGAG+TGG 0.636105 6_4:-40282619 None:intergenic
TATTTGTATCCAGGCAAGAG+TGG 0.639070 6_4:+40283038 Msa0988030:CDS
ACCATTCTTGGAAAGAACCA+AGG 0.640231 6_4:+40282305 Msa0988030:CDS
GGCCCACCACCATCAACCAA+TGG 0.643022 6_4:+40282375 Msa0988030:CDS
AGTCAACCAAACCCTTCCAT+CGG 0.647784 6_4:+40282560 Msa0988030:CDS
AAATTAGGTAAAGATGAAGA+GGG 0.651013 6_4:+40279643 Msa0988030:CDS
ATGTTCCATCAAGGACAGCA+TGG 0.653199 6_4:-40282218 None:intergenic
CAAAAGCTATGTGAAACCTG+AGG 0.658022 6_4:+40279450 Msa0988030:CDS
GAGGCAAAGGTTGAACCCGA+AGG 0.658181 6_4:+40282444 Msa0988030:CDS
AGTGAACCCACCAATGCCGG+AGG 0.665412 6_4:+40282710 Msa0988030:CDS
CCCACCATCAGTCAAAGGTG+AGG 0.665634 6_4:+40282470 Msa0988030:CDS
TGGGCCGAGATATGCAGTGG+TGG 0.665900 6_4:+40282264 Msa0988030:CDS
ACCACCATCAACCAATGGTG+AGG 0.669817 6_4:+40282380 Msa0988030:CDS
TCATGGGCCGAGATATGCAG+TGG 0.679492 6_4:+40282261 Msa0988030:CDS
TGGAGCCTGAGGCTATGGGA+AGG 0.683457 6_4:+40283090 Msa0988030:CDS
ATTACCTGAACAGCCAACAA+GGG 0.693693 6_4:-40279829 None:intergenic
ATCCAGGCAAGAGTGGAGGG+AGG 0.695098 6_4:+40283045 Msa0988030:CDS
TGAAGTCATGAAGTGCCACG+TGG 0.764930 6_4:-40282241 None:intergenic
TCCTTGATGGAACATCCACG+TGG 0.766828 6_4:+40282226 Msa0988030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGTAAAAAATTAATTTT+TGG - chr6_4:40279543-40279562 None:intergenic 10.0%
!!! CAAAAATTAATTTTTTACAA+AGG + chr6_4:40279541-40279560 Msa0988030:CDS 10.0%
!! AAATTTAATCAATTTAGAAA+TGG - chr6_4:40279913-40279932 None:intergenic 10.0%
!! ATTTATTGATATTCTATTAT+TGG - chr6_4:40280513-40280532 None:intergenic 10.0%
!! ATAATAGAATATCAATAAAT+AGG + chr6_4:40280513-40280532 Msa0988030:intron 10.0%
!!! CTTTTGTTTATATTTTATTT+TGG + chr6_4:40280879-40280898 Msa0988030:intron 10.0%
!!! GGAATTAAAATTTATTTTTT+GGG - chr6_4:40281246-40281265 None:intergenic 10.0%
!! AAAAAGTTATAATTTATAAG+AGG + chr6_4:40281742-40281761 Msa0988030:intron 10.0%
!!! TTATCTTGTTTTTAAATATT+CGG - chr6_4:40282030-40282049 None:intergenic 10.0%
!! AGTTAATATTATCTTAATAA+TGG + chr6_4:40283369-40283388 Msa0988030:three_prime_UTR 10.0%
!!! AAATTTTAAGAAGTTTTTCT+TGG - chr6_4:40279366-40279385 None:intergenic 15.0%
!!! AAATTAATTTTTTACAAAGG+AGG + chr6_4:40279544-40279563 Msa0988030:CDS 15.0%
!! CTATGATTTCAAATATTTAA+TGG - chr6_4:40280116-40280135 None:intergenic 15.0%
!! TTACATTTATAGTTAATATG+TGG + chr6_4:40280210-40280229 Msa0988030:intron 15.0%
!! TACATTTATAGTTAATATGT+GGG + chr6_4:40280211-40280230 Msa0988030:intron 15.0%
!! TTTACATTATATTTACCTTA+AGG + chr6_4:40280678-40280697 Msa0988030:intron 15.0%
!!! TTGTTTATATTTTATTTTGG+AGG + chr6_4:40280882-40280901 Msa0988030:intron 15.0%
!!! CGGAATTAAAATTTATTTTT+TGG - chr6_4:40281247-40281266 None:intergenic 15.0%
!!! TTTATCTTATCCTTTTTTAT+TGG - chr6_4:40281313-40281332 None:intergenic 15.0%
!!! ATTAGTTTAATCATAATCAA+CGG - chr6_4:40281578-40281597 None:intergenic 15.0%
!!! AATTATTTTAACCATTTCTT+AGG - chr6_4:40281606-40281625 None:intergenic 15.0%
!!! TATAATTTATAAGAGGATTT+TGG + chr6_4:40281749-40281768 Msa0988030:intron 15.0%
!!! TATTTTTGGAAGTTATATAT+GGG - chr6_4:40281799-40281818 None:intergenic 15.0%
!!! TTATTTTTGGAAGTTATATA+TGG - chr6_4:40281800-40281819 None:intergenic 15.0%
!! TCAAAAATATTATGATGTTT+AGG + chr6_4:40283240-40283259 Msa0988030:three_prime_UTR 15.0%
!!! AATTATATTTTTAAAGAAGG+TGG - chr6_4:40283398-40283417 None:intergenic 15.0%
!! TTTATATAGTATTTGGCATT+TGG - chr6_4:40280328-40280347 None:intergenic 20.0%
!! AACCACATTTATATAGTATT+TGG - chr6_4:40280335-40280354 None:intergenic 20.0%
!! ATATTCTATTATTGGCAAAT+AGG - chr6_4:40280505-40280524 None:intergenic 20.0%
!! ATATAAACAAAAGTGATAGT+TGG - chr6_4:40280872-40280891 None:intergenic 20.0%
!!! TTTATATTTTATTTTGGAGG+AGG + chr6_4:40280885-40280904 Msa0988030:intron 20.0%
!! AGGTATTATTTAAACTCAAA+TGG + chr6_4:40280905-40280924 Msa0988030:intron 20.0%
!! CATTAAATACCTTATAGTAA+TGG + chr6_4:40281194-40281213 Msa0988030:intron 20.0%
!! ATTAAACAACCATTACTATA+AGG - chr6_4:40281206-40281225 None:intergenic 20.0%
!! TAAATCTCAACCAATAAAAA+AGG + chr6_4:40281300-40281319 Msa0988030:intron 20.0%
!! TGAAGAAAATTAAATTCACT+AGG - chr6_4:40281403-40281422 None:intergenic 20.0%
!! ATTCACTCTTAATAAACAAA+TGG - chr6_4:40281447-40281466 None:intergenic 20.0%
!!! TAATTGTGTTGTTTTGTTTT+CGG + chr6_4:40281621-40281640 Msa0988030:intron 20.0%
!!! AAAGTGAATCTCTTTATTTT+TGG - chr6_4:40281813-40281832 None:intergenic 20.0%
!! TAAATCATACAAATAAGGAA+AGG - chr6_4:40282125-40282144 None:intergenic 20.0%
!! AGTCATAAATCATACAAATA+AGG - chr6_4:40282130-40282149 None:intergenic 20.0%
!! TCTTTGAATTGAAAAATCTT+GGG - chr6_4:40282894-40282913 None:intergenic 20.0%
!! ATCTTTGAATTGAAAAATCT+TGG - chr6_4:40282895-40282914 None:intergenic 20.0%
! TTGTAGTAAAAAGCTAAGTA+AGG - chr6_4:40279520-40279539 None:intergenic 25.0%
! TAAATTAGGTAAAGATGAAG+AGG + chr6_4:40279642-40279661 Msa0988030:CDS 25.0%
! AAATTAGGTAAAGATGAAGA+GGG + chr6_4:40279643-40279662 Msa0988030:CDS 25.0%
! AAATTCAAGATTCCACTATA+AGG - chr6_4:40279793-40279812 None:intergenic 25.0%
!! TTATAGTGGAATCTTGAATT+TGG + chr6_4:40279792-40279811 Msa0988030:CDS 25.0%
! TTAAATCAAAACCTACTTGT+AGG - chr6_4:40280008-40280027 None:intergenic 25.0%
!! TATTTGAAATCATAGCCTTA+AGG + chr6_4:40280121-40280140 Msa0988030:intron 25.0%
!! ATTTGAAATCATAGCCTTAA+GGG + chr6_4:40280122-40280141 Msa0988030:intron 25.0%
!! TATGAGGCTAATAATTCTTT+TGG - chr6_4:40280262-40280281 None:intergenic 25.0%
! TGCCAAATACTATATAAATG+TGG + chr6_4:40280330-40280349 Msa0988030:intron 25.0%
! AGATTGAAAACCAAAAAACA+AGG + chr6_4:40280376-40280395 Msa0988030:intron 25.0%
! GATTGAAAACCAAAAAACAA+GGG + chr6_4:40280377-40280396 Msa0988030:intron 25.0%
!!! TAAATGTATCCCTTGTTTTT+TGG - chr6_4:40280389-40280408 None:intergenic 25.0%
! ATAGCAAATCTCTATATTTG+TGG - chr6_4:40280449-40280468 None:intergenic 25.0%
! TAAAAATCAAAACAAGACAC+AGG - chr6_4:40280477-40280496 None:intergenic 25.0%
!! TTCTACTCTAATTGTTACTT+GGG - chr6_4:40280617-40280636 None:intergenic 25.0%
!! ATGTAAAAAAGAGCGTAATT+TGG - chr6_4:40280665-40280684 None:intergenic 25.0%
! TACAAAAACTAGATACCTTA+AGG - chr6_4:40280696-40280715 None:intergenic 25.0%
! AGTTGGAAAATCAATGTATA+TGG - chr6_4:40280855-40280874 None:intergenic 25.0%
!! ACTATAAGGTATTTAATGTG+TGG - chr6_4:40281192-40281211 None:intergenic 25.0%
! ATTAAATTCACTAGGTAGAT+TGG - chr6_4:40281395-40281414 None:intergenic 25.0%
! AAATAACAAAAAGAGGACAT+AGG - chr6_4:40281841-40281860 None:intergenic 25.0%
! GGTCAAAAAATAACAAAAAG+AGG - chr6_4:40281848-40281867 None:intergenic 25.0%
!!! GTCTTATTTCATGACATTAT+TGG - chr6_4:40281869-40281888 None:intergenic 25.0%
! ATGTCATGAAATAAGACAAA+TGG + chr6_4:40281872-40281891 Msa0988030:intron 25.0%
! TAAATACACCAATGGTAATT+TGG - chr6_4:40281974-40281993 None:intergenic 25.0%
!!! CCATTTTTTAAATACACCAA+TGG - chr6_4:40281982-40282001 None:intergenic 25.0%
! CCATTGGTGTATTTAAAAAA+TGG + chr6_4:40281979-40281998 Msa0988030:intron 25.0%
! TGTAAATATGACATGTGAAA+GGG - chr6_4:40282091-40282110 None:intergenic 25.0%
! ATGTAAATATGACATGTGAA+AGG - chr6_4:40282092-40282111 None:intergenic 25.0%
!!! ATTTTTCAATTCAAAGATGC+TGG + chr6_4:40282897-40282916 Msa0988030:CDS 25.0%
!!! AAGGTTTATGACATTGATTT+TGG + chr6_4:40282957-40282976 Msa0988030:CDS 25.0%
!!! TATGACATTGATTTTGGATT+TGG + chr6_4:40282963-40282982 Msa0988030:CDS 25.0%
!!! ATGACATTGATTTTGGATTT+GGG + chr6_4:40282964-40282983 Msa0988030:CDS 25.0%
! CATTGATGTTGAATTAACAT+TGG + chr6_4:40283070-40283089 Msa0988030:CDS 25.0%
! ATTTGTGCAATAAGAGAATT+TGG + chr6_4:40283164-40283183 Msa0988030:three_prime_UTR 25.0%
! TTCTATGTTTAGATTGTTGA+AGG + chr6_4:40283190-40283209 Msa0988030:three_prime_UTR 25.0%
! TCTATGTTTAGATTGTTGAA+GGG + chr6_4:40283191-40283210 Msa0988030:three_prime_UTR 25.0%
! TTTGATATAATGTTGTGTGT+TGG + chr6_4:40283267-40283286 Msa0988030:three_prime_UTR 25.0%
! TTGATATAATGTTGTGTGTT+GGG + chr6_4:40283268-40283287 Msa0988030:three_prime_UTR 25.0%
!! TTGAACTTGGTAATAATGTT+TGG + chr6_4:40283325-40283344 Msa0988030:three_prime_UTR 25.0%
!! TGAACTTGGTAATAATGTTT+GGG + chr6_4:40283326-40283345 Msa0988030:three_prime_UTR 25.0%
!! TTCTTATAGAAAAGTGTTGC+TGG - chr6_4:40279304-40279323 None:intergenic 30.0%
TGAAACCTGAGGAAAAAATT+GGG + chr6_4:40279461-40279480 Msa0988030:CDS 30.0%
!! TTTTTCCCAATTTTTTCCTC+AGG - chr6_4:40279469-40279488 None:intergenic 30.0%
TGAGGAAAAAATTGGGAAAA+AGG + chr6_4:40279468-40279487 Msa0988030:CDS 30.0%
AGTAAAAAGCTAAGTAAGGT+AGG - chr6_4:40279516-40279535 None:intergenic 30.0%
AATGTTGAGAAACTCAAGAA+TGG + chr6_4:40279580-40279599 Msa0988030:CDS 30.0%
ATGTTGAGAAACTCAAGAAT+GGG + chr6_4:40279581-40279600 Msa0988030:CDS 30.0%
! TGTTGAATATGATGATGACT+TGG + chr6_4:40279675-40279694 Msa0988030:CDS 30.0%
AGGAATCAATTCCTTCAAAA+TGG - chr6_4:40279773-40279792 None:intergenic 30.0%
! AAGGAATTGATTCCTTATAG+TGG + chr6_4:40279778-40279797 Msa0988030:CDS 30.0%
TTTAAGTGATTCCTACAAGT+AGG + chr6_4:40279994-40280013 Msa0988030:intron 30.0%
! GGCTAATAATTCTTTTGGTA+TGG - chr6_4:40280257-40280276 None:intergenic 30.0%
! GAATTATTAGCCTCATAGTT+TGG + chr6_4:40280265-40280284 Msa0988030:intron 30.0%
CTTCTACTCTAATTGTTACT+TGG - chr6_4:40280618-40280637 None:intergenic 30.0%
AGTTGAAAAGTTTGTGTATG+TGG + chr6_4:40280816-40280835 Msa0988030:intron 30.0%
! ATTAGTTTCACGTTTATGCA+TGG + chr6_4:40281059-40281078 Msa0988030:intron 30.0%
!!! AGATCTTGTTTTATGCAAAC+CGG - chr6_4:40281092-40281111 None:intergenic 30.0%
! ATTTTAGATCAATCCACACA+AGG + chr6_4:40281126-40281145 Msa0988030:intron 30.0%
!!! ATCTTTTTTGACTCAATGTG+TGG - chr6_4:40281166-40281185 None:intergenic 30.0%
! AACTGCAACTAATTTTTCTG+CGG - chr6_4:40281267-40281286 None:intergenic 30.0%
ACTAATTGTAGCCTAAGAAA+TGG + chr6_4:40281592-40281611 Msa0988030:intron 30.0%
CATACAAATAAGGAAAGGAA+AGG - chr6_4:40282120-40282139 None:intergenic 30.0%
AGCTAACAAAGCTTAAAGAT+GGG + chr6_4:40282172-40282191 Msa0988030:intron 30.0%
!!! AAGATTTTTTCCCTAAGTAC+GGG - chr6_4:40282501-40282520 None:intergenic 30.0%
!!! GAAGATTTTTTCCCTAAGTA+CGG - chr6_4:40282502-40282521 None:intergenic 30.0%
AAAATCATTCTCAACTTTCC+AGG + chr6_4:40282590-40282609 Msa0988030:CDS 30.0%
TTGATCCAAAAGGTAATTGA+AGG + chr6_4:40282819-40282838 Msa0988030:CDS 30.0%
ATGTCATAAACCTTAAACCT+AGG - chr6_4:40282951-40282970 None:intergenic 30.0%
GAACCAACAACAAATTTGAT+GGG + chr6_4:40283009-40283028 Msa0988030:CDS 30.0%
GCAAGATAAGGAATTTCTTA+TGG + chr6_4:40283118-40283137 Msa0988030:CDS 30.0%
TCTCTTATTGCACAAATTCT+TGG - chr6_4:40283161-40283180 None:intergenic 30.0%
GCTTTGATTGAATTTGAACT+TGG + chr6_4:40283312-40283331 Msa0988030:three_prime_UTR 30.0%
GCATGCATTTATGGAATTCA+TGG - chr6_4:40279282-40279301 None:intergenic 35.0%
! TGTGTGAGATGTGATTTTTG+AGG - chr6_4:40279328-40279347 None:intergenic 35.0%
GTGAAACCTGAGGAAAAAAT+TGG + chr6_4:40279460-40279479 Msa0988030:CDS 35.0%
!! TGGGAAAAAGGAGTATCAAT+TGG + chr6_4:40279480-40279499 Msa0988030:CDS 35.0%
CATCAACTAGCTGGTAAATT+AGG + chr6_4:40279628-40279647 Msa0988030:CDS 35.0%
!! GAATATGATGATGACTTGGT+TGG + chr6_4:40279679-40279698 Msa0988030:CDS 35.0%
!!! TGAAAGTAGTGCCATTTTGA+AGG + chr6_4:40279759-40279778 Msa0988030:CDS 35.0%
AGTGGAATCTTGAATTTGGA+AGG + chr6_4:40279796-40279815 Msa0988030:CDS 35.0%
AAGTTGAATGTTGACCCTTA+AGG - chr6_4:40280139-40280158 None:intergenic 35.0%
GACTCTTTAACCAAACTATG+AGG - chr6_4:40280278-40280297 None:intergenic 35.0%
CACACATAGAACTTGATCTA+AGG + chr6_4:40280732-40280751 Msa0988030:intron 35.0%
!!! GATCTTGTTTTATGCAAACC+GGG - chr6_4:40281091-40281110 None:intergenic 35.0%
!! TTTTTGACTCAATGTGTGGT+TGG - chr6_4:40281162-40281181 None:intergenic 35.0%
AAAATTGAGCAATGCACACT+TGG - chr6_4:40281354-40281373 None:intergenic 35.0%
!!! TTGTGTTGTTTTGTTTTCGG+TGG + chr6_4:40281624-40281643 Msa0988030:intron 35.0%
GCAAATGTAACAAGGATTTC+GGG - chr6_4:40281701-40281720 None:intergenic 35.0%
CGCAAATGTAACAAGGATTT+CGG - chr6_4:40281702-40281721 None:intergenic 35.0%
TGGAAGTTATATATGGGAAG+AGG - chr6_4:40281793-40281812 None:intergenic 35.0%
ACCAATGGTAATTTGGCAAA+CGG - chr6_4:40281967-40281986 None:intergenic 35.0%
!! CATCTTTAAGCTTTGTTAGC+TGG - chr6_4:40282173-40282192 None:intergenic 35.0%
CAGCTAACAAAGCTTAAAGA+TGG + chr6_4:40282171-40282190 Msa0988030:intron 35.0%
!!! TGTTGACTTGATTTTGTCGA+TGG - chr6_4:40282541-40282560 None:intergenic 35.0%
CGGTGAATATAGCTTGAATT+AGG - chr6_4:40282749-40282768 None:intergenic 35.0%
TCAAGCTATATTCACCGTTA+CGG + chr6_4:40282752-40282771 Msa0988030:CDS 35.0%
!! CATCAATAGCTTTAGCATCA+TGG - chr6_4:40282845-40282864 None:intergenic 35.0%
TTGATGAACGTAACAAAGAG+TGG + chr6_4:40282859-40282878 Msa0988030:CDS 35.0%
TGATGAACGTAACAAAGAGT+GGG + chr6_4:40282860-40282879 Msa0988030:CDS 35.0%
GGAACCAACAACAAATTTGA+TGG + chr6_4:40283008-40283027 Msa0988030:CDS 35.0%
!! CATCCCATCAAATTTGTTGT+TGG - chr6_4:40283015-40283034 None:intergenic 35.0%
GGGATGATTTATTTGTATCC+AGG + chr6_4:40283029-40283048 Msa0988030:CDS 35.0%
!! TGTGTGTTGGGTTTTGTATT+TGG + chr6_4:40283280-40283299 Msa0988030:three_prime_UTR 35.0%
CAAAAGCTATGTGAAACCTG+AGG + chr6_4:40279450-40279469 Msa0988030:CDS 40.0%
!!! TGGGCTTAGTGTAGTTTTGA+AGG + chr6_4:40279600-40279619 Msa0988030:CDS 40.0%
AAGGAGTTTCATCAACTAGC+TGG + chr6_4:40279619-40279638 Msa0988030:CDS 40.0%
ATTACCTGAACAGCCAACAA+GGG - chr6_4:40279832-40279851 None:intergenic 40.0%
AATTACCTGAACAGCCAACA+AGG - chr6_4:40279833-40279852 None:intergenic 40.0%
! TGGGTTGAGAGATCATAGTT+TGG + chr6_4:40280230-40280249 Msa0988030:intron 40.0%
GTTTATGCATGGAGAATACC+CGG + chr6_4:40281070-40281089 Msa0988030:intron 40.0%
CTTAGTACTAGAGCAATGCA+TGG + chr6_4:40281540-40281559 Msa0988030:intron 40.0%
ACCGTTTGCCAAATTACCAT+TGG + chr6_4:40281963-40281982 Msa0988030:intron 40.0%
!! CTTAAAGATGGGCTAGCAAT+GGG + chr6_4:40282183-40282202 Msa0988030:CDS 40.0%
TTTAACCATGCTGTCCTTGA+TGG + chr6_4:40282213-40282232 Msa0988030:CDS 40.0%
GGTTCTTTCCAAGAATGGTT+GGG - chr6_4:40282304-40282323 None:intergenic 40.0%
TGGTTCTTTCCAAGAATGGT+TGG - chr6_4:40282305-40282324 None:intergenic 40.0%
ACCATTCTTGGAAAGAACCA+AGG + chr6_4:40282305-40282324 Msa0988030:CDS 40.0%
! TTTACCTCACCATTGGTTGA+TGG - chr6_4:40282387-40282406 None:intergenic 40.0%
ATCAACCAATGGTGAGGTAA+AGG + chr6_4:40282386-40282405 Msa0988030:CDS 40.0%
TTCATCCTTTACCTCACCAT+TGG - chr6_4:40282394-40282413 None:intergenic 40.0%
GAGGTAAAGGATGAACAGAA+TGG + chr6_4:40282399-40282418 Msa0988030:CDS 40.0%
! AATGATTTTGAACCGTCCGA+TGG - chr6_4:40282579-40282598 None:intergenic 40.0%
TGTGAACACTGTGTAGTCTT+CGG - chr6_4:40282667-40282686 None:intergenic 40.0%
! CATGGCCTTCAATTACCTTT+TGG - chr6_4:40282827-40282846 None:intergenic 40.0%
TGGAAGTTCACCTAGGTTTA+AGG + chr6_4:40282938-40282957 Msa0988030:CDS 40.0%
TATTTGTATCCAGGCAAGAG+TGG + chr6_4:40283038-40283057 Msa0988030:CDS 40.0%
TGAATTAACATTGGAGCCTG+AGG + chr6_4:40283079-40283098 Msa0988030:CDS 40.0%
!! GTGTTGGGTTTTGTATTTGG+TGG + chr6_4:40283283-40283302 Msa0988030:three_prime_UTR 40.0%
! GTGTTGCTGGCATGCATTTA+TGG - chr6_4:40279291-40279310 None:intergenic 45.0%
GAAGCTGTTGCTGATGAGAT+TGG + chr6_4:40279715-40279734 Msa0988030:CDS 45.0%
TGGCATTTGGAGAGAACTCT+AGG - chr6_4:40280315-40280334 None:intergenic 45.0%
! TGGCTGATCTTTTCCTTGTG+TGG - chr6_4:40281142-40281161 None:intergenic 45.0%
ACTAGAGCAATGCATGGCTT+TGG + chr6_4:40281546-40281565 Msa0988030:intron 45.0%
! GTAACAAGGATTTCGGGTGT+CGG - chr6_4:40281695-40281714 None:intergenic 45.0%
CATCACGCGCAAATGTAACA+AGG - chr6_4:40281709-40281728 None:intergenic 45.0%
!! GCTTAAAGATGGGCTAGCAA+TGG + chr6_4:40282182-40282201 Msa0988030:CDS 45.0%
ATGTTCCATCAAGGACAGCA+TGG - chr6_4:40282221-40282240 None:intergenic 45.0%
GTGGCACTTCATGACTTCAT+GGG + chr6_4:40282245-40282264 Msa0988030:CDS 45.0%
AATGGTTGGGCTGTTGTGAT+GGG - chr6_4:40282291-40282310 None:intergenic 45.0%
! GCCTTGGTTCTTTCCAAGAA+TGG - chr6_4:40282309-40282328 None:intergenic 45.0%
AAGCTTCACACGTGTGTTTC+GGG - chr6_4:40282331-40282350 None:intergenic 45.0%
CAAGCTTCACACGTGTGTTT+CGG - chr6_4:40282332-40282351 None:intergenic 45.0%
TTCAACCTTTGCCTCACCAT+TGG - chr6_4:40282439-40282458 None:intergenic 45.0%
! GATTTTGTCGATGGCTGACT+CGG - chr6_4:40282532-40282551 None:intergenic 45.0%
AGTCAACCAAACCCTTCCAT+CGG + chr6_4:40282560-40282579 Msa0988030:CDS 45.0%
! TTTTGAACCGTCCGATGGAA+GGG - chr6_4:40282574-40282593 None:intergenic 45.0%
! ATTTTGAACCGTCCGATGGA+AGG - chr6_4:40282575-40282594 None:intergenic 45.0%
AAATATGAGTGGAGAGAGCC+TGG - chr6_4:40282611-40282630 None:intergenic 45.0%
AGGCTCTCTCCACTCATATT+TGG + chr6_4:40282610-40282629 Msa0988030:CDS 45.0%
GTGAACACTGTGTAGTCTTC+GGG - chr6_4:40282666-40282685 None:intergenic 45.0%
AGGTTTCCAAAGTATGCCTC+CGG - chr6_4:40282729-40282748 None:intergenic 45.0%
!! ATCAAAGAAGCGCCGAATTG+TGG - chr6_4:40282804-40282823 None:intergenic 45.0%
GGTGTGAATTGTGTTGCAGT+TGG + chr6_4:40282918-40282937 Msa0988030:CDS 45.0%
TTGCAGTTGGAAGTTCACCT+AGG + chr6_4:40282931-40282950 Msa0988030:CDS 45.0%
AAGCCAGAGATTGTGAGAAG+TGG + chr6_4:40282987-40283006 Msa0988030:CDS 45.0%
GTTCCACTTCTCACAATCTC+TGG - chr6_4:40282993-40283012 None:intergenic 45.0%
!!! AGTATATTTTAATTTAAAAA+TGG - chr6_4:40279974-40279993 None:intergenic 5.0%
!!! AAATTTTTTTAAATCTAAAT+TGG - chr6_4:40281035-40281054 None:intergenic 5.0%
!! TTTAGATTTAAAAAAATTTA+AGG + chr6_4:40281036-40281055 Msa0988030:intron 5.0%
AGGCATGCATAGACCCTTGT+TGG + chr6_4:40279816-40279835 Msa0988030:CDS 50.0%
! TAGACCCTTGTTGGCTGTTC+AGG + chr6_4:40279825-40279844 Msa0988030:CDS 50.0%
TCCTTGATGGAACATCCACG+TGG + chr6_4:40282226-40282245 Msa0988030:CDS 50.0%
! TGAAGTCATGAAGTGCCACG+TGG - chr6_4:40282244-40282263 None:intergenic 50.0%
CGTGGCACTTCATGACTTCA+TGG + chr6_4:40282244-40282263 Msa0988030:CDS 50.0%
GAATGGTTGGGCTGTTGTGA+TGG - chr6_4:40282292-40282311 None:intergenic 50.0%
CACAACAGCCCAACCATTCT+TGG + chr6_4:40282293-40282312 Msa0988030:CDS 50.0%
!! CACCATTGGTTGATGGTGGT+GGG - chr6_4:40282380-40282399 None:intergenic 50.0%
!! TCACCATTGGTTGATGGTGG+TGG - chr6_4:40282381-40282400 None:intergenic 50.0%
ACCACCATCAACCAATGGTG+AGG + chr6_4:40282380-40282399 Msa0988030:CDS 50.0%
!! ACCTCACCATTGGTTGATGG+TGG - chr6_4:40282384-40282403 None:intergenic 50.0%
! TTTGCCTCACCATTGGCTGA+TGG - chr6_4:40282432-40282451 None:intergenic 50.0%
ATCAGCCAATGGTGAGGCAA+AGG + chr6_4:40282431-40282450 Msa0988030:CDS 50.0%
!! TCACCTTTGACTGATGGTGG+GGG - chr6_4:40282471-40282490 None:intergenic 50.0%
! CTCACCTTTGACTGATGGTG+GGG - chr6_4:40282472-40282491 None:intergenic 50.0%
CCTCACCTTTGACTGATGGT+GGG - chr6_4:40282473-40282492 None:intergenic 50.0%
CTTGCCTCACCTTTGACTGA+TGG - chr6_4:40282477-40282496 None:intergenic 50.0%
CATCAGTCAAAGGTGAGGCA+AGG + chr6_4:40282475-40282494 Msa0988030:CDS 50.0%
AACCAAACCCTTCCATCGGA+CGG + chr6_4:40282564-40282583 Msa0988030:CDS 50.0%
!!! CGGGTTTTAGTTCACGTGCA+AGG - chr6_4:40282647-40282666 None:intergenic 50.0%
ATATTCACCGTTACGGCTGC+TGG + chr6_4:40282759-40282778 Msa0988030:CDS 50.0%
TATTCACCGTTACGGCTGCT+GGG + chr6_4:40282760-40282779 Msa0988030:CDS 50.0%
!! AAAGAAGCGCCGAATTGTGG+CGG - chr6_4:40282801-40282820 None:intergenic 50.0%
CGGCGCTTCTTTGATCCAAA+AGG + chr6_4:40282809-40282828 Msa0988030:CDS 50.0%
TTGTATCCAGGCAAGAGTGG+AGG + chr6_4:40283041-40283060 Msa0988030:CDS 50.0%
TGTATCCAGGCAAGAGTGGA+GGG + chr6_4:40283042-40283061 Msa0988030:CDS 50.0%
AACATTGGAGCCTGAGGCTA+TGG + chr6_4:40283085-40283104 Msa0988030:CDS 50.0%
ACATTGGAGCCTGAGGCTAT+GGG + chr6_4:40283086-40283105 Msa0988030:CDS 50.0%
GGGAAGGTTGGAGCAAGATA+AGG + chr6_4:40283106-40283125 Msa0988030:CDS 50.0%
!! AGATGGGCTAGCAATGGGCT+TGG + chr6_4:40282188-40282207 Msa0988030:CDS 55.0%
GCCACGTGGATGTTCCATCA+AGG - chr6_4:40282230-40282249 None:intergenic 55.0%
TCATGGGCCGAGATATGCAG+TGG + chr6_4:40282261-40282280 Msa0988030:CDS 55.0%
GGGTCCACCACTGCATATCT+CGG - chr6_4:40282271-40282290 None:intergenic 55.0%
!! GTTGATGGTGGTGGGCCTTT+GGG - chr6_4:40282372-40282391 None:intergenic 55.0%
! CACCATTGGCTGATGGTGCT+GGG - chr6_4:40282425-40282444 None:intergenic 55.0%
! TCACCATTGGCTGATGGTGC+TGG - chr6_4:40282426-40282445 None:intergenic 55.0%
AGCACCATCAGCCAATGGTG+AGG + chr6_4:40282425-40282444 Msa0988030:CDS 55.0%
GAGGCAAAGGTTGAACCCGA+AGG + chr6_4:40282444-40282463 Msa0988030:CDS 55.0%
CCCACCATCAGTCAAAGGTG+AGG + chr6_4:40282470-40282489 Msa0988030:CDS 55.0%
GCCTCACCTTTGACTGATGG+TGG - chr6_4:40282474-40282493 None:intergenic 55.0%
TGAGGCAAGGCCCGTACTTA+GGG + chr6_4:40282488-40282507 Msa0988030:CDS 55.0%
AACCGTCCGATGGAAGGGTT+TGG - chr6_4:40282569-40282588 None:intergenic 55.0%
CACGTGGCGCCAAATATGAG+TGG - chr6_4:40282622-40282641 None:intergenic 55.0%
ACGAGTGAACCCACCAATGC+CGG + chr6_4:40282707-40282726 Msa0988030:CDS 55.0%
!! AGTATGCCTCCGGCATTGGT+GGG - chr6_4:40282719-40282738 None:intergenic 55.0%
! AAGTATGCCTCCGGCATTGG+TGG - chr6_4:40282720-40282739 None:intergenic 55.0%
CCAAAGTATGCCTCCGGCAT+TGG - chr6_4:40282723-40282742 None:intergenic 55.0%
! CCAATGCCGGAGGCATACTT+TGG + chr6_4:40282720-40282739 Msa0988030:CDS 55.0%
CAAAAGCCCAGCAGCCGTAA+CGG - chr6_4:40282769-40282788 None:intergenic 55.0%
!! AAGAAGCGCCGAATTGTGGC+GGG - chr6_4:40282800-40282819 None:intergenic 55.0%
TCCAACCTTCCCATAGCCTC+AGG - chr6_4:40283098-40283117 None:intergenic 55.0%
TGGGCCGAGATATGCAGTGG+TGG + chr6_4:40282264-40282283 Msa0988030:CDS 60.0%
CACACGTGTGTTTCGGGCCT+TGG - chr6_4:40282325-40282344 None:intergenic 60.0%
!! GGTTGATGGTGGTGGGCCTT+TGG - chr6_4:40282373-40282392 None:intergenic 60.0%
GGCCCACCACCATCAACCAA+TGG + chr6_4:40282375-40282394 Msa0988030:CDS 60.0%
!! ACTGATGGTGGGGGACCTTC+GGG - chr6_4:40282462-40282481 None:intergenic 60.0%
!! GACTGATGGTGGGGGACCTT+CGG - chr6_4:40282463-40282482 None:intergenic 60.0%
GGTCCCCCACCATCAGTCAA+AGG + chr6_4:40282465-40282484 Msa0988030:CDS 60.0%
GTGAGGCAAGGCCCGTACTT+AGG + chr6_4:40282487-40282506 Msa0988030:CDS 60.0%
GTTCACGTGCAAGGCTCACG+TGG - chr6_4:40282638-40282657 None:intergenic 60.0%
AGTGAACCCACCAATGCCGG+AGG + chr6_4:40282710-40282729 Msa0988030:CDS 60.0%
! CGTTACGGCTGCTGGGCTTT+TGG + chr6_4:40282767-40282786 Msa0988030:CDS 60.0%
! TACGGCTGCTGGGCTTTTGG+CGG + chr6_4:40282770-40282789 Msa0988030:CDS 60.0%
ATCCAGGCAAGAGTGGAGGG+AGG + chr6_4:40283045-40283064 Msa0988030:CDS 60.0%
TGGAGCCTGAGGCTATGGGA+AGG + chr6_4:40283090-40283109 Msa0988030:CDS 60.0%
GCCTGAGGCTATGGGAAGGT+TGG + chr6_4:40283094-40283113 Msa0988030:CDS 60.0%
CTTTGGGCTCGGGTAGGGAG+AGG - chr6_4:40282356-40282375 None:intergenic 65.0%
CTCTCCCTACCCGAGCCCAA+AGG + chr6_4:40282354-40282373 Msa0988030:CDS 65.0%
TGGGCCTTTGGGCTCGGGTA+GGG - chr6_4:40282361-40282380 None:intergenic 65.0%
!! TGGTGGTGGGCCTTTGGGCT+CGG - chr6_4:40282367-40282386 None:intergenic 65.0%
GGCCCAGCACCATCAGCCAA+TGG + chr6_4:40282420-40282439 Msa0988030:CDS 65.0%
GCGGCTCACCCGCCACAATT+CGG + chr6_4:40282789-40282808 Msa0988030:CDS 65.0%
CTCCTCCCTCCACTCTTGCC+TGG - chr6_4:40283050-40283069 None:intergenic 65.0%
GTGGGCCTTTGGGCTCGGGT+AGG - chr6_4:40282362-40282381 None:intergenic 70.0%
!! GGTGGTGGGCCTTTGGGCTC+GGG - chr6_4:40282366-40282385 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 40279274 40283418 40279274 ID=Msa0988030;Name=Msa0988030
chr6_4 mRNA 40279274 40283418 40279274 ID=Msa0988030-mRNA-1;Parent=Msa0988030;Name=Msa0988030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=24|1|1|1|1|1|2|270|507
chr6_4 exon 40279274 40279846 40279274 ID=Msa0988030-mRNA-1:exon:9761;Parent=Msa0988030-mRNA-1
chr6_4 exon 40282174 40283418 40282174 ID=Msa0988030-mRNA-1:exon:9762;Parent=Msa0988030-mRNA-1
chr6_4 five_prime_UTR 40279274 40279297 40279274 ID=Msa0988030-mRNA-1:five_prime_utr;Parent=Msa0988030-mRNA-1
chr6_4 CDS 40279298 40279846 40279298 ID=Msa0988030-mRNA-1:cds;Parent=Msa0988030-mRNA-1
chr6_4 CDS 40282174 40283148 40282174 ID=Msa0988030-mRNA-1:cds;Parent=Msa0988030-mRNA-1
chr6_4 three_prime_UTR 40283149 40283418 40283149 ID=Msa0988030-mRNA-1:three_prime_utr;Parent=Msa0988030-mRNA-1
Gene Sequence

>Msa0988030

ATGCCAGCAACACTTTTCTATAAGAATCCTCAAAAATCACATCTCACACACAAATCAAACAAATTCCAAGAAAAACTTCTTAAAATTTCATTCAACAAAATGTCAAGTGAAATAGTCAAGAAAGAAGAATCTCTAAATGTGAAAATCACTAGCAAAAGCTATGTGAAACCTGAGGAAAAAATTGGGAAAAAGGAGTATCAATTGGTCACATTTGACCTACCTTACTTAGCTTTTTACTACAACCAAAAATTAATTTTTTACAAAGGAGGTAACTTTGAAGAAAATGTTGAGAAACTCAAGAATGGGCTTAGTGTAGTTTTGAAGGAGTTTCATCAACTAGCTGGTAAATTAGGTAAAGATGAAGAGGGTGTGTTTTTTGTTGAATATGATGATGACTTGGTTGGTGTTGAAGTTGTTGAAGCTGTTGCTGATGAGATTGGTGTTGAAGATCTAACTGTTGCTGAAAGTAGTGCCATTTTGAAGGAATTGATTCCTTATAGTGGAATCTTGAATTTGGAAGGCATGCATAGACCCTTGTTGGCTGTTCAGCTAACAAAGCTTAAAGATGGGCTAGCAATGGGCTTGGCTTTTAACCATGCTGTCCTTGATGGAACATCCACGTGGCACTTCATGACTTCATGGGCCGAGATATGCAGTGGTGGACCCATCACAACAGCCCAACCATTCTTGGAAAGAACCAAGGCCCGAAACACACGTGTGAAGCTTGACCTCTCCCTACCCGAGCCCAAAGGCCCACCACCATCAACCAATGGTGAGGTAAAGGATGAACAGAATGGCCCAGCACCATCAGCCAATGGTGAGGCAAAGGTTGAACCCGAAGGTCCCCCACCATCAGTCAAAGGTGAGGCAAGGCCCGTACTTAGGGAAAAAATCTTCAAATTTTCCGAGTCAGCCATCGACAAAATCAAGTCAACAGTCAACCAAACCCTTCCATCGGACGGTTCAAAATCATTCTCAACTTTCCAGGCTCTCTCCACTCATATTTGGCGCCACGTGAGCCTTGCACGTGAACTAAAACCCGAAGACTACACAGTGTTCACAGTCTTCGCAGACTGTAGAAAACGAGTGAACCCACCAATGCCGGAGGCATACTTTGGAAACCTAATTCAAGCTATATTCACCGTTACGGCTGCTGGGCTTTTGGCGGCTCACCCGCCACAATTCGGCGCTTCTTTGATCCAAAAGGTAATTGAAGGCCATGATGCTAAAGCTATTGATGAACGTAACAAAGAGTGGGAGAGTGCACCCAAGATTTTTCAATTCAAAGATGCTGGTGTGAATTGTGTTGCAGTTGGAAGTTCACCTAGGTTTAAGGTTTATGACATTGATTTTGGATTTGGGAAGCCAGAGATTGTGAGAAGTGGAACCAACAACAAATTTGATGGGATGATTTATTTGTATCCAGGCAAGAGTGGAGGGAGGAGCATTGATGTTGAATTAACATTGGAGCCTGAGGCTATGGGAAGGTTGGAGCAAGATAAGGAATTTCTTATGGAAGTATAA
Protein sequence

>Msa0988030

MPATLFYKNPQKSHLTHKSNKFQEKLLKISFNKMSSEIVKKEESLNVKITSKSYVKPEEKIGKKEYQLVTFDLPYLAFYYNQKLIFYKGGNFEENVEKLKNGLSVVLKEFHQLAGKLGKDEEGVFFVEYDDDLVGVEVVEAVADEIGVEDLTVAESSAILKELIPYSGILNLEGMHRPLLAVQLTKLKDGLAMGLAFNHAVLDGTSTWHFMTSWAEICSGGPITTAQPFLERTKARNTRVKLDLSLPEPKGPPPSTNGEVKDEQNGPAPSANGEAKVEPEGPPPSVKGEARPVLREKIFKFSESAIDKIKSTVNQTLPSDGSKSFSTFQALSTHIWRHVSLARELKPEDYTVFTVFADCRKRVNPPMPEAYFGNLIQAIFTVTAAGLLAAHPPQFGASLIQKVIEGHDAKAIDERNKEWESAPKIFQFKDAGVNCVAVGSSPRFKVYDIDFGFGKPEIVRSGTNNKFDGMIYLYPGKSGGRSIDVELTLEPEAMGRLEQDKEFLMEV*