AlfalfaGEDB | Msa1462920

M. sativa cultivar zhongmu-4 / Msa1462920

KEGG: K14684
GO: GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006091 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0008150 GO:0008152 GO:0008509 GO:0008514 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009579 GO:0009605 GO:0009607 GO:0009624 GO:0009628 GO:0009765 GO:0009941 GO:0009987 GO:0010117 GO:0010206 GO:0015075 GO:0015215 GO:0015216 GO:0015238 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0015979 GO:0016020 GO:0016021 GO:0019538 GO:0019684 GO:0019866 GO:0022857 GO:0030091 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0034220 GO:0034357 GO:0042221 GO:0042493 GO:0042651 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0051704 GO:0051707 GO:0055085 GO:0071702 GO:0071704 GO:0071705 GO:0098656 GO:1901264 GO:1901505 GO:1901564 GO:1901679
PFAM: PF00153.30

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462920	AES86901.2	93.023	129	9	0	1	129	1	129	8.84e-63	206

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462920	sp\|O65023\|EAAC_ARATH	70.732	82	24	0	48	129	50	131	3.80e-24	98.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462920	G7JP47	93.023	129	9	0	1	129	1	129	4.22e-63	206

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0037650	Msa1462920	0.821545	3.814608e-53	-8.615850e-47
Msa0067750	Msa1462920	0.800454	1.430454e-48	-8.615850e-47
Msa1356100	Msa1462920	0.800373	1.485795e-48	-8.615850e-47
Msa1372470	Msa1462920	0.814361	1.602145e-51	-8.615850e-47
Msa1373570	Msa1462920	0.802862	4.586204e-49	-8.615850e-47
Msa1375980	Msa1462920	0.830865	2.308731e-55	-8.615850e-47
Msa1377980	Msa1462920	0.827183	1.800765e-54	-8.615850e-47
Msa1382100	Msa1462920	0.808321	3.280774e-50	-8.615850e-47
Msa1398450	Msa1462920	0.812378	4.369495e-51	-8.615850e-47
Msa1403820	Msa1462920	0.801903	7.227086e-49	-8.615850e-47
Msa1408280	Msa1462920	0.827327	1.663106e-54	-8.615850e-47
Msa1434850	Msa1462920	0.805165	1.522659e-49	-8.615850e-47
Msa0610300	Msa1462920	0.803191	3.921954e-49	-8.615850e-47
Msa0615430	Msa1462920	0.804209	2.410953e-49	-8.615850e-47
Msa0616890	Msa1462920	0.800807	1.212023e-48	-8.615850e-47
Msa0662670	Msa1462920	0.808891	2.478304e-50	-8.615850e-47
Msa0688880	Msa1462920	0.811002	8.704485e-51	-8.615850e-47
Msa0698480	Msa1462920	0.805061	1.600679e-49	-8.615850e-47
Msa0705710	Msa1462920	0.821375	4.174276e-53	-8.615850e-47
Msa0708870	Msa1462920	0.811457	6.933910e-51	-8.615850e-47
Msa0840450	Msa1462920	0.804429	2.169452e-49	-8.615850e-47
Msa0165820	Msa1462920	0.812501	4.107963e-51	-8.615850e-47
Msa0189150	Msa1462920	0.813383	2.631236e-51	-8.615850e-47
Msa0225070	Msa1462920	0.807599	4.673331e-50	-8.615850e-47
Msa0236690	Msa1462920	0.809827	1.560914e-50	-8.615850e-47
Msa1269370	Msa1462920	0.804052	2.599082e-49	-8.615850e-47
Msa1291070	Msa1462920	0.802500	5.446594e-49	-8.615850e-47
Msa1341390	Msa1462920	0.818817	1.608260e-52	-8.615850e-47
Msa0392120	Msa1462920	0.805598	1.235804e-49	-8.615850e-47
Msa0450230	Msa1462920	0.821735	3.446782e-53	-8.615850e-47
Msa0450430	Msa1462920	0.811571	6.550986e-51	-8.615850e-47
Msa0470080	Msa1462920	0.825887	3.669070e-54	-8.615850e-47
Msa0471480	Msa1462920	0.807216	5.632745e-50	-8.615850e-47
Msa1084210	Msa1462920	0.800627	1.318725e-48	-8.615850e-47
Msa1088000	Msa1462920	0.817831	2.689559e-52	-8.615850e-47
Msa0266140	Msa1462920	0.814278	1.671659e-51	-8.615850e-47
Msa0269660	Msa1462920	0.808834	2.548755e-50	-8.615850e-47
Msa0270730	Msa1462920	0.804133	2.500240e-49	-8.615850e-47
Msa0290800	Msa1462920	-0.802020	6.839136e-49	-8.615850e-47
Msa0310260	Msa1462920	0.807356	5.261937e-50	-8.615850e-47
Msa0328870	Msa1462920	-0.807251	5.538527e-50	-8.615850e-47
Msa0330770	Msa1462920	0.825290	5.082007e-54	-8.615850e-47
Msa1109500	Msa1462920	0.808269	3.365427e-50	-8.615850e-47
Msa1121720	Msa1462920	0.800236	1.584029e-48	-8.615850e-47
Msa1128230	Msa1462920	0.809344	1.982263e-50	-8.615850e-47
Msa1169600	Msa1462920	0.801107	1.052613e-48	-8.615850e-47
Msa1222890	Msa1462920	0.813038	3.132522e-51	-8.615850e-47
Msa0511040	Msa1462920	0.806095	9.714347e-50	-8.615850e-47
Msa0538050	Msa1462920	0.812549	4.009778e-51	-8.615850e-47
Msa0568300	Msa1462920	0.803744	3.011283e-49	-8.615850e-47
Msa0572730	Msa1462920	0.820555	6.449411e-53	-8.615850e-47
Msa0582270	Msa1462920	0.810974	8.825000e-51	-8.615850e-47
Msa0588110	Msa1462920	0.805885	1.075606e-49	-8.615850e-47
Msa0872890	Msa1462920	0.832438	9.452707e-56	-8.615850e-47
Msa0880310	Msa1462920	0.803905	2.788169e-49	-8.615850e-47
Msa0898830	Msa1462920	0.807722	4.398474e-50	-8.615850e-47
Msa0967900	Msa1462920	0.801218	9.987363e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462920	MtrunA17_Chr4g0006851	93.023	129	9	0	1	129	1	129	2.11e-66	205
Msa1462920	MtrunA17_Chr7g0242311	59.091	132	46	3	1	129	1	127	4.51e-35	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1462920	AT3G51870.1	70.732	82	24	0	48	129	50	131	3.86e-25	98.2
Msa1462920	AT5G01500.1	75.000	80	20	0	50	129	80	159	1.85e-23	94.0

Find 49 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTAGAGGTGGTGGTTAAAT+TGG	0.220101	tig0042795:-11321	Msa1462920:intergenic
GGTCGAGACGGTGGCGAATT+TGG	0.227999	tig0042795:-11417	Msa1462920:intergenic
TTGTTCGTTGTGTCTGTCAT+AGG	0.236276	tig0042795:-11272	Msa1462920:intergenic
AGGGATAAGTTTGATGGTTT+TGG	0.270207	tig0042795:-11252	Msa1462920:intergenic
TACTGCGTGTGTTTGCTTAA+TGG	0.274617	tig0042795:-11215	Msa1462920:intergenic
GAGAAACAATGCAGCATCTT+TGG	0.280947	tig0042795:-11522	Msa1462920:intergenic
ATTCGTCGTTCGGTTTGGTT+TGG	0.338959	tig0042795:-11442	Msa1462920:intergenic
TTCGTCGTTCGGTTTGGTTT+GGG	0.339031	tig0042795:-11441	Msa1462920:intergenic
TTTCTCGCCGGTGCTGTTGC+TGG	0.345164	tig0042795:+11539	Msa1462920:CDS
TTAGAGGTGGTGGTTAAATT+GGG	0.348256	tig0042795:-11320	Msa1462920:intergenic
TAAGAAGCTGAGCCGGCGTC+GGG	0.359061	tig0042795:-11469	Msa1462920:intergenic
CAGACTCATGGAGTGCGAAT+TGG	0.377216	tig0042795:+11698	Msa1462920:intron
GTCGGGGCGAATTCGTCGTT+CGG	0.390883	tig0042795:-11452	Msa1462920:intergenic
AGAAACAATGCAGCATCTTT+GGG	0.402197	tig0042795:-11521	Msa1462920:intergenic
TGTCATAGGGATAAGTTTGA+TGG	0.405847	tig0042795:-11258	Msa1462920:intergenic
GGCGAATTCGTCGTTCGGTT+TGG	0.417389	tig0042795:-11447	Msa1462920:intergenic
AGCAGCTCCAGCAACAGCAC+CGG	0.449657	tig0042795:-11546	Msa1462920:intergenic
CAGTTGTTGAAGAGGTGGTT+GGG	0.453871	tig0042795:-11356	Msa1462920:intergenic
GGGTGAAACGACGTCGTTAG+AGG	0.465059	tig0042795:-11336	Msa1462920:intergenic
CAAGTGCGACGACGGCGATC+GGG	0.465236	tig0042795:-11496	Msa1462920:intergenic
GCAGTTGTTGAAGAGGTGGT+TGG	0.471890	tig0042795:-11357	Msa1462920:intergenic
ACAAGTGCGACGACGGCGAT+CGG	0.484450	tig0042795:-11497	Msa1462920:intergenic
TGTTCGTTGTGTCTGTCATA+GGG	0.497418	tig0042795:-11271	Msa1462920:intergenic
TTAAGAAGCTGAGCCGGCGT+CGG	0.508929	tig0042795:-11470	Msa1462920:intergenic
TCGAATCAAGCTTCTCATGC+AGG	0.519660	tig0042795:+11595	Msa1462920:CDS
GAGACGGTGGCGAATTTGGA+AGG	0.527604	tig0042795:-11413	Msa1462920:intergenic
AACGACGTCGTTAGAGGTGG+TGG	0.529351	tig0042795:-11330	Msa1462920:intergenic
TAGAGGTGGTGGTTAAATTG+GGG	0.531755	tig0042795:-11319	Msa1462920:intergenic
TTTGGTTTGGGAGGTCGAGA+CGG	0.537437	tig0042795:-11429	Msa1462920:intergenic
TCGATCAAGTGGAGCAGTGA+CGG	0.556672	tig0042795:-11576	Msa1462920:intergenic
AGAAGCTTGATTCGATCAAG+TGG	0.560589	tig0042795:-11587	Msa1462920:intergenic
CGACGAATTCGCCCCGACGC+CGG	0.564909	tig0042795:+11457	Msa1462920:CDS
GATGTTACGCCACGTGGCGA+CGG	0.567513	tig0042795:-11297	Msa1462920:intergenic
GGTGGCGAATTTGGAAGGAA+TGG	0.574574	tig0042795:-11408	Msa1462920:intergenic
TGAAGGTGCAGTTGTTGAAG+AGG	0.579448	tig0042795:-11364	Msa1462920:intergenic
ATTGGGGATGTTACGCCACG+TGG	0.585033	tig0042795:-11303	Msa1462920:intergenic
GATGCTGCATTGTTTCTCGC+CGG	0.586437	tig0042795:+11527	Msa1462920:CDS
GGGTGGTTAAGAAGCTGAGC+CGG	0.587213	tig0042795:-11476	Msa1462920:intergenic
GTCGTTCGGTTTGGTTTGGG+AGG	0.587458	tig0042795:-11438	Msa1462920:intergenic
AGGTGCAGTTGTTGAAGAGG+TGG	0.589863	tig0042795:-11361	Msa1462920:intergenic
GTGCGACGACGGCGATCGGG+TGG	0.593456	tig0042795:-11493	Msa1462920:intergenic
GAATTTGGAAGGAATGGTGG+TGG	0.593957	tig0042795:-11402	Msa1462920:intergenic
GGCGAATTTGGAAGGAATGG+TGG	0.612416	tig0042795:-11405	Msa1462920:intergenic
TTTGGGAACAAGTGCGACGA+CGG	0.629042	tig0042795:-11504	Msa1462920:intergenic
GGTTTGGGAGGTCGAGACGG+TGG	0.634889	tig0042795:-11426	Msa1462920:intergenic
CGAACAAAACCGTCGCCACG+TGG	0.655549	tig0042795:+11288	Msa1462920:CDS
TGAAACGACGTCGTTAGAGG+TGG	0.660799	tig0042795:-11333	Msa1462910:intergenic
AAGAAGCTGAGCCGGCGTCG+GGG	0.667870	tig0042795:-11468	Msa1462910:intergenic
GGTTGCGTCGAGTAAAGTGA+AGG	0.718129	tig0042795:-11381	Msa1462910:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTACTACAAATCTCTCAAAA+TGG	-	tig0042795:11644-11663	Msa1462920:intergenic	25.0%
	GATGTTTATGAAATGCATTC+TGG	+	tig0042795:11668-11687	Msa1462920:intron	30.0%
!!	AGGGATAAGTTTGATGGTTT+TGG	-	tig0042795:11255-11274	Msa0131100:intergenic	35.0%
	TGTCATAGGGATAAGTTTGA+TGG	-	tig0042795:11261-11280	Msa0131100:intergenic	35.0%
!	TTAGAGGTGGTGGTTAAATT+GGG	-	tig0042795:11323-11342	Msa1462920:intergenic	35.0%
	AGAAACAATGCAGCATCTTT+GGG	-	tig0042795:11524-11543	Msa1462920:intergenic	35.0%
	TACTGCGTGTGTTTGCTTAA+TGG	-	tig0042795:11218-11237	Msa0131100:intergenic	40.0%
	TGTTCGTTGTGTCTGTCATA+GGG	-	tig0042795:11274-11293	Msa0131100:intergenic	40.0%
	TTGTTCGTTGTGTCTGTCAT+AGG	-	tig0042795:11275-11294	Msa0131100:intergenic	40.0%
!	TAGAGGTGGTGGTTAAATTG+GGG	-	tig0042795:11322-11341	Msa1462920:intergenic	40.0%
!	GTTAGAGGTGGTGGTTAAAT+TGG	-	tig0042795:11324-11343	Msa1462920:intergenic	40.0%
	GAGAAACAATGCAGCATCTT+TGG	-	tig0042795:11525-11544	Msa1462920:intergenic	40.0%
!	AGAAGCTTGATTCGATCAAG+TGG	-	tig0042795:11590-11609	Msa1462920:intergenic	40.0%
!!!	TCTGGTTTTTTGCAGACTCA+TGG	+	tig0042795:11686-11705	Msa1462920:intron	40.0%
	CAGTTGTTGAAGAGGTGGTT+GGG	-	tig0042795:11359-11378	Msa1462920:intergenic	45.0%
!	TGAAGGTGCAGTTGTTGAAG+AGG	-	tig0042795:11367-11386	Msa1462920:intergenic	45.0%
	GAATTTGGAAGGAATGGTGG+TGG	-	tig0042795:11405-11424	Msa1462920:intergenic	45.0%
!!	TTCGTCGTTCGGTTTGGTTT+GGG	-	tig0042795:11444-11463	Msa1462920:intergenic	45.0%
!!	ATTCGTCGTTCGGTTTGGTT+TGG	-	tig0042795:11445-11464	Msa1462920:intergenic	45.0%
	TCGAATCAAGCTTCTCATGC+AGG	+	tig0042795:11595-11614	Msa1462920:CDS	45.0%
	TGAAACGACGTCGTTAGAGG+TGG	-	tig0042795:11336-11355	Msa1462920:intergenic	50.0%
	GCAGTTGTTGAAGAGGTGGT+TGG	-	tig0042795:11360-11379	Msa1462920:intergenic	50.0%
	AGGTGCAGTTGTTGAAGAGG+TGG	-	tig0042795:11364-11383	Msa1462920:intergenic	50.0%
	GGTTGCGTCGAGTAAAGTGA+AGG	-	tig0042795:11384-11403	Msa1462920:intergenic	50.0%
	GGCGAATTTGGAAGGAATGG+TGG	-	tig0042795:11408-11427	Msa1462920:intergenic	50.0%
	GGTGGCGAATTTGGAAGGAA+TGG	-	tig0042795:11411-11430	Msa1462920:intergenic	50.0%
!!	TTTGGTTTGGGAGGTCGAGA+CGG	-	tig0042795:11432-11451	Msa1462920:intergenic	50.0%
	TTTGGGAACAAGTGCGACGA+CGG	-	tig0042795:11507-11526	Msa1462920:intergenic	50.0%
!	GATGCTGCATTGTTTCTCGC+CGG	+	tig0042795:11527-11546	Msa1462920:CDS	50.0%
	TCGATCAAGTGGAGCAGTGA+CGG	-	tig0042795:11579-11598	Msa1462920:intergenic	50.0%
	CAGACTCATGGAGTGCGAAT+TGG	+	tig0042795:11698-11717	Msa1462920:intron	50.0%
	ATTGGGGATGTTACGCCACG+TGG	-	tig0042795:11306-11325	Msa1462920:intergenic	55.0%
	AACGACGTCGTTAGAGGTGG+TGG	-	tig0042795:11333-11352	Msa1462920:intergenic	55.0%
	GGGTGAAACGACGTCGTTAG+AGG	-	tig0042795:11339-11358	Msa1462920:intergenic	55.0%
	GAGACGGTGGCGAATTTGGA+AGG	-	tig0042795:11416-11435	Msa1462920:intergenic	55.0%
!!	GTCGTTCGGTTTGGTTTGGG+AGG	-	tig0042795:11441-11460	Msa1462920:intergenic	55.0%
	GGCGAATTCGTCGTTCGGTT+TGG	-	tig0042795:11450-11469	Msa1462920:intergenic	55.0%
	TTAAGAAGCTGAGCCGGCGT+CGG	-	tig0042795:11473-11492	Msa1462920:intergenic	55.0%
	GGGTGGTTAAGAAGCTGAGC+CGG	-	tig0042795:11479-11498	Msa1462920:intergenic	55.0%
	CGAACAAAACCGTCGCCACG+TGG	+	tig0042795:11288-11307	Msa1462920:CDS	60.0%
	GATGTTACGCCACGTGGCGA+CGG	-	tig0042795:11300-11319	Msa1462920:intergenic	60.0%
	GGTCGAGACGGTGGCGAATT+TGG	-	tig0042795:11420-11439	Msa1462920:intergenic	60.0%
	GTCGGGGCGAATTCGTCGTT+CGG	-	tig0042795:11455-11474	Msa1462920:intergenic	60.0%
	TAAGAAGCTGAGCCGGCGTC+GGG	-	tig0042795:11472-11491	Msa1462920:intergenic	60.0%
	ACAAGTGCGACGACGGCGAT+CGG	-	tig0042795:11500-11519	Msa1462920:intergenic	60.0%
!!	TTTCTCGCCGGTGCTGTTGC+TGG	+	tig0042795:11539-11558	Msa1462920:CDS	60.0%
	AGCAGCTCCAGCAACAGCAC+CGG	-	tig0042795:11549-11568	Msa1462920:intergenic	60.0%
	GGTTTGGGAGGTCGAGACGG+TGG	-	tig0042795:11429-11448	Msa1462920:intergenic	65.0%
	AAGAAGCTGAGCCGGCGTCG+GGG	-	tig0042795:11471-11490	Msa1462920:intergenic	65.0%
	CAAGTGCGACGACGGCGATC+GGG	-	tig0042795:11499-11518	Msa1462920:intergenic	65.0%
	CGACGAATTCGCCCCGACGC+CGG	+	tig0042795:11457-11476	Msa1462920:CDS	70.0%
	GTGCGACGACGGCGATCGGG+TGG	-	tig0042795:11496-11515	Msa1462920:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
tig0042795	gene	11192	11745	11192	ID=Msa1462920;Name=Msa1462920
tig0042795	mRNA	11192	11745	11192	ID=Msa1462920-mRNA-1;Parent=Msa1462920;Name=Msa1462920-mRNA-1;_AED=0.00;_eAED=0.00;_QI=83\|1\|1\|1\|1\|1\|2\|0\|129
tig0042795	exon	11192	11616	11192	ID=Msa1462920-mRNA-1:exon:23831;Parent=Msa1462920-mRNA-1
tig0042795	exon	11701	11745	11701	ID=Msa1462920-mRNA-1:exon:23832;Parent=Msa1462920-mRNA-1
tig0042795	five_prime_UTR	11192	11274	11192	ID=Msa1462920-mRNA-1:five_prime_utr;Parent=Msa1462920-mRNA-1
tig0042795	CDS	11275	11616	11275	ID=Msa1462920-mRNA-1:cds;Parent=Msa1462920-mRNA-1
tig0042795	CDS	11701	11745	11701	ID=Msa1462920-mRNA-1:cds;Parent=Msa1462920-mRNA-1

Gene Sequence

>Msa1462920

ATGACAGACACAACGAACAAAACCGTCGCCACGTGGCGTAACATCCCCAATTTAACCACCACCTCTAACGACGTCGTTTCACCCAACCACCTCTTCAACAACTGCACCTTCACTTTACTCGACGCAACCACCACCATTCCTTCCAAATTCGCCACCGTCTCGACCTCCCAAACCAAACCGAACGACGAATTCGCCCCGACGCCGGCTCAGCTTCTTAACCACCCGATCGCCGTCGTCGCACTTGTTCCCAAAGATGCTGCATTGTTTCTCGCCGGTGCTGTTGCTGGAGCTGCTGCTAAAACCGTCACTGCTCCACTTGATCGAATCAAGCTTCTCATGCAGACTCATGGAGTGCGAATTGGACAAGAAAGTGCTAAGAAGACAATT

Protein sequence

>Msa1462920

MTDTTNKTVATWRNIPNLTTTSNDVVSPNHLFNNCTFTLLDATTTIPSKFATVSTSQTKPNDEFAPTPAQLLNHPIAVVALVPKDAALFLAGAVAGAAAKTVTAPLDRIKLLMQTHGVRIGQESAKKTI