Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572730 | RHN43474.1 | 90.240 | 584 | 11 | 3 | 1 | 547 | 1 | 575 | 0.0 | 1048 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572730 | A0A396GVF7 | 90.240 | 584 | 11 | 3 | 1 | 547 | 1 | 575 | 0.0 | 1048 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000610 | Msa0572730 | 0.886100 | 4.588298e-72 | -8.615850e-47 |
| Msa0000800 | Msa0572730 | -0.854341 | 1.313082e-61 | -8.615850e-47 |
| Msa0001040 | Msa0572730 | 0.804462 | 2.135675e-49 | -8.615850e-47 |
| Msa0001510 | Msa0572730 | 0.895673 | 7.665200e-76 | -8.615850e-47 |
| Msa0001550 | Msa0572730 | 0.826791 | 2.235028e-54 | -8.615850e-47 |
| Msa0002110 | Msa0572730 | 0.898939 | 3.244732e-77 | -8.615850e-47 |
| Msa0002690 | Msa0572730 | 0.919774 | 2.955912e-87 | -8.615850e-47 |
| Msa0002700 | Msa0572730 | 0.919387 | 4.796611e-87 | -8.615850e-47 |
| Msa0003340 | Msa0572730 | 0.865414 | 6.006994e-65 | -8.615850e-47 |
| Msa0004180 | Msa0572730 | 0.923845 | 1.551817e-89 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572730 | MtrunA17_Chr8g0388251 | 90.240 | 584 | 11 | 3 | 1 | 547 | 1 | 575 | 0.0 | 1048 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572730 | AT1G64430.1 | 61.988 | 513 | 140 | 5 | 64 | 541 | 67 | 559 | 0.0 | 632 |
| Msa0572730 | AT1G64430.2 | 61.988 | 513 | 140 | 5 | 64 | 541 | 67 | 559 | 0.0 | 632 |
| Msa0572730 | AT1G64430.3 | 59.479 | 422 | 116 | 5 | 64 | 450 | 67 | 468 | 8.07e-171 | 492 |
Find 151 sgRNAs with CRISPR-Local
Find 401 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGACAAAGCAAGTTG+TGG | 0.586674 | 4_1:-75651594 | None:intergenic |
| AAACAAGTTCATATTGGTAG+CGG | 0.603374 | 4_1:-75653058 | None:intergenic |
| AAAGGGTGAGGAAGTGTGCG+TGG | 0.557503 | 4_1:-75651270 | None:intergenic |
| AAATCCGCTGTTAACCATCC+TGG | 0.533493 | 4_1:+75656928 | Msa0572730:three_prime_UTR |
| AAATCTTTAAACTTCGTCCA+AGG | 0.510030 | 4_1:-75656605 | None:intergenic |
| AAATTTGTCTGCTAAAAGAC+AGG | 0.529388 | 4_1:-75656727 | None:intergenic |
| AACAAACCACAAAGATGGAC+GGG | 0.544397 | 4_1:+75654281 | Msa0572730:CDS |
| AAGAAGTGGAAAGAGGATGG+AGG | 0.531428 | 4_1:-75654358 | None:intergenic |
| AAGGGTGAGGAAGTGTGCGT+GGG | 0.618174 | 4_1:-75651269 | None:intergenic |
| AATACCAGGATGGTTAACAG+CGG | 0.773507 | 4_1:-75656932 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAAGAATAAAAAGGGTG+AGG | - | chr4_1:75651285-75651304 | None:intergenic | 30.0% | |
| AATTATCTGCAATGCTTCAT+CGG | - | chr4_1:75651490-75651509 | None:intergenic | 30.0% | |
| TTTGTATACACTGTAACAGT+TGG | + | chr4_1:75652021-75652040 | Msa0572730:intron | 30.0% | |
| AATGTATAAACACGTGCATT+TGG | - | chr4_1:75652087-75652106 | None:intergenic | 30.0% | |
| CGTGGTTAAATATATATGTG+AGG | + | chr4_1:75652155-75652174 | Msa0572730:CDS | 30.0% | |
| GGTTAAATATATATGTGAGG+TGG | + | chr4_1:75652158-75652177 | Msa0572730:CDS | 30.0% | |
| TAAACTTATCCTTCTTCACA+AGG | - | chr4_1:75652701-75652720 | None:intergenic | 30.0% | |
| AAACAAGTTCATATTGGTAG+CGG | - | chr4_1:75653061-75653080 | None:intergenic | 30.0% | |
| TTCCTGAAACAAGTTCATAT+TGG | - | chr4_1:75653067-75653086 | None:intergenic | 30.0% | |
| GGCAAATACTTCGAAAAAAA+AGG | - | chr4_1:75653266-75653285 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 75651240 | 75657058 | 75651240 | ID=Msa0572730;Name=Msa0572730 |
| chr4_1 | mRNA | 75651240 | 75657058 | 75651240 | ID=Msa0572730-mRNA-1;Parent=Msa0572730;Name=Msa0572730-mRNA-1;_AED=0.22;_eAED=0.23;_QI=0|0.81|0.75|1|0.90|0.83|12|437|547 |
| chr4_1 | exon | 75651240 | 75651611 | 75651240 | ID=Msa0572730-mRNA-1:exon:26232;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75652117 | 75652200 | 75652117 | ID=Msa0572730-mRNA-1:exon:26233;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75652312 | 75652389 | 75652312 | ID=Msa0572730-mRNA-1:exon:26234;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75653038 | 75653149 | 75653038 | ID=Msa0572730-mRNA-1:exon:26235;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75653386 | 75653492 | 75653386 | ID=Msa0572730-mRNA-1:exon:26236;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75654096 | 75654515 | 75654096 | ID=Msa0572730-mRNA-1:exon:26237;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75654839 | 75654946 | 75654839 | ID=Msa0572730-mRNA-1:exon:26238;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75655366 | 75655433 | 75655366 | ID=Msa0572730-mRNA-1:exon:26239;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75655597 | 75655684 | 75655597 | ID=Msa0572730-mRNA-1:exon:26240;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75655804 | 75655875 | 75655804 | ID=Msa0572730-mRNA-1:exon:26241;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75655959 | 75656012 | 75655959 | ID=Msa0572730-mRNA-1:exon:26242;Parent=Msa0572730-mRNA-1 |
| chr4_1 | exon | 75656541 | 75657058 | 75656541 | ID=Msa0572730-mRNA-1:exon:26243;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75651240 | 75651611 | 75651240 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75652117 | 75652200 | 75652117 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75652312 | 75652389 | 75652312 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75653038 | 75653149 | 75653038 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75653386 | 75653492 | 75653386 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75654096 | 75654515 | 75654096 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75654839 | 75654946 | 75654839 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75655366 | 75655433 | 75655366 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75655597 | 75655684 | 75655597 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75655804 | 75655875 | 75655804 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75655959 | 75656012 | 75655959 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | CDS | 75656541 | 75656621 | 75656541 | ID=Msa0572730-mRNA-1:cds;Parent=Msa0572730-mRNA-1 |
| chr4_1 | three_prime_UTR | 75656622 | 75657058 | 75656622 | ID=Msa0572730-mRNA-1:three_prime_utr;Parent=Msa0572730-mRNA-1 |
| Gene Sequence |
>Msa0572730 ATGATTCTAAACATATCAACACCTTCATTCCCACGCACACTTCCTCACCCTTTTTATTCTTCTTTCAAACTCTCTGAAACTTATTCCAACCGCAACAACAGTTCTTATCCACTTTTCATTTTCACAAAACCATTGAAATTCATCAACCTCAAAGCTTCCGTTAGCGAAAGCCAAAATGAAACATCAAACTCTAACAACTTATTGGATCAACAGCTTCTTCTCCGAGTTGCCTCCACCGCCAAAGACGCCGATGAAGCATTGCAGATAATTGCTGACAACTCTTCCACTAATGGTGGTGTTGTTTCTACTTCTGATTGTTGCTCTATTATTTCTGTTGCGCTTGATAGGAATAATCCACAACTTGCTTTGTCTGGTTTGGCAGCATCTTTGAGGGTTTCAGATGCTCTTAGCGTGGTTAAATATATATGTGAGGTGGGCGTGTCTCCTTCTGAGGAGGTTCCCTTTGGGAAGATAGTGAGGTGTCCTACTTGTCGGATAGCTGTTGCAGTTGCACAGCCACAACAAGGTATTCAGATAGTATCTTGTGCAAAGTGCCGCTACCAATATGAACTTGTTTCAGGAAACATAGTCAATATTCAATCAGAAGAAATCAGGTTGGTTAGTCATATCATTTACTCTATGAGCAGATCTCCTCCTGCCGAACGCAGCATGGACATTACAGCATGGAAAAAGGGGTTAAGATTCCTGAAACTGATGAAGCAAAGCATTCCATCTGCTGTTCACTCTATTGTGGTGCAGACGCCTTCTGGTATGGCTCGTACTCACAGATTTGCAACTGAGACAGTTGATCTGCCAGCACAAGAAGGCGAAAGGGTAACTGTTGCCTTGGCAGCTCCATCAAATGTATATAGAAAGCTGGGCCCCATTAAATTAAGTTCAAGAGCCCCGGATTTATATCCTGGTGAAGCTATGTGCATAACAAACCACAAAGATGGACGGGAATCACGTCTTGTAAGAGCCCCTACAAAAGATGAAAATTCATCATTACTTAAACCCTCCATCCTCTTTCCACTTCTTGCTTTGTTTGCCACTGGTGACGCTGCTTCTGGACTTATTGACCCCAGCCTGCCTCAATTCCTATCAGTTGTTGCAGTTTCATCTCTTGCTATTGGCTCTGCCTTGAACTCCTTTGTTTTTCCCCAGTTTAATCAGCTTCCGCAAAGATCGGTGGAAGTAACTGCTATCAAACAACGTCTTTTATCTCAATATGACATGCTTCAGTCTCGCATCAATGACTTAAAAGAAGCTGCTGAAAAAGAGGTTTGGATGTTAGCCCGGATGTGCCAACTTGAGAATAAAATTTCAGCTGTTGGAGAACCTTCTTATCGGACTCGGATAAGTAAAGTCAAAAGGGTCCGAGAGAGCCTGCAAAATTCCCTCCAGGGACGAATTGAATTGATTGCTAGCTATGCTAGGATTTCCTCTATGATTGAAATTGAAGTGGAGATGGAAACTGATGTTCTTGCTGCTGAAACTACTAGTGATGTGGATGGGTTCACAGAACAGATAGAGCAAATCATGGAGCTTGAAAATTTAGAGGAGAGATGGAAAATGCAAGCAGAAGCAAATGATGAGGCCGAAAGACTTCTTAGCTCCCAGCCAGTGCCCTTGGACGAAGTTTAA |
| Protein sequence |
>Msa0572730 MILNISTPSFPRTLPHPFYSSFKLSETYSNRNNSSYPLFIFTKPLKFINLKASVSESQNETSNSNNLLDQQLLLRVASTAKDADEALQIIADNSSTNGGVVSTSDCCSIISVALDRNNPQLALSGLAASLRVSDALSVVKYICEVGVSPSEEVPFGKIVRCPTCRIAVAVAQPQQGIQIVSCAKCRYQYELVSGNIVNIQSEEIRLVSHIIYSMSRSPPAERSMDITAWKKGLRFLKLMKQSIPSAVHSIVVQTPSGMARTHRFATETVDLPAQEGERVTVALAAPSNVYRKLGPIKLSSRAPDLYPGEAMCITNHKDGRESRLVRAPTKDENSSLLKPSILFPLLALFATGDAASGLIDPSLPQFLSVVAVSSLAIGSALNSFVFPQFNQLPQRSVEVTAIKQRLLSQYDMLQSRINDLKEAAEKEVWMLARMCQLENKISAVGEPSYRTRISKVKRVRESLQNSLQGRIELIASYARISSMIEIEVEMETDVLAAETTSDVDGFTEQIEQIMELENLEERWKMQAEANDEAERLLSSQPVPLDEV* |
