AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0270660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0270660 MtrunA17_Chr2g0277971 97.959 147 3 0 1 147 1 147 3.84e-101 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0270660 AT1G60870.1 59.184 147 60 0 1 147 1 147 1.64e-33 115

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGAGAAAGCAAGAAATA+TGG 0.282094 2_3:-1900376 Msa0270660:CDS
GCTTGGAAAGGAATTTCTTC+TGG 0.317736 2_3:-1900267 Msa0270660:CDS
AGCAGCTGCCCATGCTTTGT+AGG 0.349595 2_3:+1900560 None:intergenic
AAGAAATATGGGAAACTTGA+TGG 0.389367 2_3:-1900364 Msa0270660:CDS
GTGCCATGGATGAATTTAGA+AGG 0.394252 2_3:-1900455 Msa0270660:CDS
GGAATATCTCGACTCGGCTA+TGG 0.422650 2_3:-1900481 Msa0270660:CDS
GAGGATTTCAAGAGATGAAG+TGG 0.462068 2_3:-1900415 Msa0270660:CDS
GCTGAGAAAGCAAGAAATAT+GGG 0.463308 2_3:-1900375 Msa0270660:CDS
ATAAGATCTTCGATCGTCGA+TGG 0.506443 2_3:+1900603 None:intergenic
GAAAGGAATTTCTTCTGGCA+AGG 0.513277 2_3:-1900262 Msa0270660:CDS
AGATAGAATCCTACAAAGCA+TGG 0.513381 2_3:-1900569 Msa0270660:CDS
AAAGGAAGGTGAACTGAGAA+AGG 0.516993 2_3:+1900658 None:intergenic
CTGCTTCTATGAAATCTGCT+TGG 0.529588 2_3:-1900284 Msa0270660:CDS
GGTTTGAAGAAGAACTTGAG+AGG 0.547329 2_3:-1900434 Msa0270660:CDS
AAACCTTCTAAATTCATCCA+TGG 0.552800 2_3:+1900452 None:intergenic
TAAAGATTGATCTGAAAGGA+AGG 0.570141 2_3:+1900644 None:intergenic
AAAGTATATATGTATCAAGA+TGG 0.576287 2_3:+1900234 None:intergenic
CTCGGCTATGGAAAGTGCCA+TGG 0.577831 2_3:-1900469 Msa0270660:CDS
TCTATGAAATCTGCTTGGAA+AGG 0.578134 2_3:-1900279 Msa0270660:CDS
CATGTAAAGATTGATCTGAA+AGG 0.594535 2_3:+1900640 None:intergenic
AGCCGAGTCGAGATATTCCT+CGG 0.604035 2_3:+1900485 None:intergenic
TGAACTTGAGCAAGAAAGAG+AGG 0.624850 2_3:-1900538 Msa0270660:CDS
AGCCGAGGAATATCTCGACT+CGG 0.631008 2_3:-1900487 Msa0270660:CDS
GATAGAATCCTACAAAGCAT+GGG 0.638623 2_3:-1900568 Msa0270660:CDS
AGATGCAATGCAAGAAGCCG+AGG 0.640190 2_3:-1900502 Msa0270660:CDS
ACTTGAGCAAGAAAGAGAGG+TGG 0.641638 2_3:-1900535 Msa0270660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAAAGATTGATCTGAAAGGA+AGG + chr2_3:1900276-1900295 None:intergenic 30.0%
CATGTAAAGATTGATCTGAA+AGG + chr2_3:1900280-1900299 None:intergenic 30.0%
AAACCTTCTAAATTCATCCA+TGG + chr2_3:1900468-1900487 None:intergenic 30.0%
AAGAAATATGGGAAACTTGA+TGG - chr2_3:1900553-1900572 Msa0270660:CDS 30.0%
AGATAGAATCCTACAAAGCA+TGG - chr2_3:1900348-1900367 Msa0270660:CDS 35.0%
GATAGAATCCTACAAAGCAT+GGG - chr2_3:1900349-1900368 Msa0270660:CDS 35.0%
AGCTGAGAAAGCAAGAAATA+TGG - chr2_3:1900541-1900560 Msa0270660:CDS 35.0%
GCTGAGAAAGCAAGAAATAT+GGG - chr2_3:1900542-1900561 Msa0270660:CDS 35.0%
TCTATGAAATCTGCTTGGAA+AGG - chr2_3:1900638-1900657 Msa0270660:CDS 35.0%
AAAGGAAGGTGAACTGAGAA+AGG + chr2_3:1900262-1900281 None:intergenic 40.0%
ATAAGATCTTCGATCGTCGA+TGG + chr2_3:1900317-1900336 None:intergenic 40.0%
TGAACTTGAGCAAGAAAGAG+AGG - chr2_3:1900379-1900398 Msa0270660:CDS 40.0%
GTGCCATGGATGAATTTAGA+AGG - chr2_3:1900462-1900481 Msa0270660:CDS 40.0%
GGTTTGAAGAAGAACTTGAG+AGG - chr2_3:1900483-1900502 Msa0270660:CDS 40.0%
GAGGATTTCAAGAGATGAAG+TGG - chr2_3:1900502-1900521 Msa0270660:CDS 40.0%
CTGCTTCTATGAAATCTGCT+TGG - chr2_3:1900633-1900652 Msa0270660:CDS 40.0%
GCTTGGAAAGGAATTTCTTC+TGG - chr2_3:1900650-1900669 Msa0270660:CDS 40.0%
GAAAGGAATTTCTTCTGGCA+AGG - chr2_3:1900655-1900674 Msa0270660:CDS 40.0%
ACTTGAGCAAGAAAGAGAGG+TGG - chr2_3:1900382-1900401 Msa0270660:CDS 45.0%
AGATGCAATGCAAGAAGCCG+AGG - chr2_3:1900415-1900434 Msa0270660:CDS 50.0%
AGCCGAGGAATATCTCGACT+CGG - chr2_3:1900430-1900449 Msa0270660:CDS 50.0%
AGCCGAGTCGAGATATTCCT+CGG + chr2_3:1900435-1900454 None:intergenic 50.0%
!! GGAATATCTCGACTCGGCTA+TGG - chr2_3:1900436-1900455 Msa0270660:CDS 50.0%
AGCAGCTGCCCATGCTTTGT+AGG + chr2_3:1900360-1900379 None:intergenic 55.0%
!! CTCGGCTATGGAAAGTGCCA+TGG - chr2_3:1900448-1900467 Msa0270660:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 1900248 1900691 1900248 ID=Msa0270660;Name=Msa0270660
chr2_3 mRNA 1900248 1900691 1900248 ID=Msa0270660-mRNA-1;Parent=Msa0270660;Name=Msa0270660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|147
chr2_3 exon 1900248 1900691 1900248 ID=Msa0270660-mRNA-1:exon:986;Parent=Msa0270660-mRNA-1
chr2_3 CDS 1900248 1900691 1900248 ID=Msa0270660-mRNA-1:cds;Parent=Msa0270660-mRNA-1
Gene Sequence

>Msa0270660

ATGGAAGCTCTCCTTTCTCAGTTCACCTTCCTTTCAGATCAATCTTTACATGACAAGAGCTTCGATCCATCGACGATCGAAGATCTTATGAAACTATTTGAGATAGAATCCTACAAAGCATGGGCAGCTGCTGAACTTGAGCAAGAAAGAGAGGTGGAAGAAGCAGAAGATGCAATGCAAGAAGCCGAGGAATATCTCGACTCGGCTATGGAAAGTGCCATGGATGAATTTAGAAGGTTTGAAGAAGAACTTGAGAGGATTTCAAGAGATGAAGTGGAGAGTTTAGTTCAAACAGCTGAGAAAGCAAGAAATATGGGAAACTTGATGGAAAAAACAGCTTCTATTGCTTCTAAGAAATATATTGAAGCTGCACTTAATTCAGCTACTGCTTCTATGAAATCTGCTTGGAAAGGAATTTCTTCTGGCAAGGTTCATCCATCTTGA

Protein sequence

>Msa0270660

MEALLSQFTFLSDQSLHDKSFDPSTIEDLMKLFEIESYKAWAAAELEQEREVEEAEDAMQEAEEYLDSAMESAMDEFRRFEEELERISRDEVESLVQTAEKARNMGNLMEKTASIASKKYIEAALNSATASMKSAWKGISSGKVHPS*