AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0705820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705820 MtrunA17_Chr8g0384311 97.479 119 3 0 168 286 90 208 6.13e-81 243
Msa0705820 MtrunA17_Chr8g0384311 100.000 90 0 0 1 90 1 90 1.24e-57 183
Msa0705820 MtrunA17_Chr3g0130271 92.373 118 8 1 170 286 91 208 4.82e-75 228
Msa0705820 MtrunA17_Chr3g0130271 87.912 91 10 1 1 91 1 90 6.00e-48 159
Msa0705820 MtrunA17_Chr1g0159481 83.333 120 19 1 168 286 94 213 3.80e-69 213
Msa0705820 MtrunA17_Chr1g0159481 91.463 82 7 0 9 90 13 94 3.29e-47 157
Msa0705820 MtrunA17_Chr1g0180291 65.833 120 40 1 168 286 91 210 5.40e-54 174
Msa0705820 MtrunA17_Chr1g0180291 71.605 81 23 0 10 90 11 91 5.82e-35 125
Msa0705820 MtrunA17_Chr7g0265001 62.810 121 42 2 168 286 94 213 8.08e-53 171
Msa0705820 MtrunA17_Chr7g0265001 73.171 82 21 1 10 90 13 94 5.91e-35 125
Msa0705820 MtrunA17_Chr1g0197331 58.824 119 48 1 168 286 94 211 2.03e-43 147
Msa0705820 MtrunA17_Chr1g0197331 67.021 94 27 2 1 90 1 94 1.29e-33 122
Msa0705820 MtrunA17_Chr2g0276931 70.238 84 24 1 8 90 4 87 1.61e-34 124
Msa0705820 MtrunA17_Chr2g0276931 35.294 119 74 3 168 286 87 202 6.71e-14 68.9
Msa0705820 MtrunA17_Chr4g0055651 69.880 83 24 1 9 90 4 86 8.80e-34 122
Msa0705820 MtrunA17_Chr4g0055651 31.933 119 77 3 168 286 86 200 1.07e-13 68.6
Msa0705820 MtrunA17_Chr8g0335471 69.880 83 24 1 9 90 4 86 2.01e-33 121
Msa0705820 MtrunA17_Chr8g0335471 32.773 119 77 3 168 286 86 201 1.60e-12 65.1
Msa0705820 MtrunA17_Chr7g0250531 69.880 83 24 1 9 90 4 86 2.24e-33 121
Msa0705820 MtrunA17_Chr7g0250531 34.454 119 74 3 168 286 86 200 2.27e-14 70.5
Msa0705820 MtrunA17_Chr1g0182321 56.818 88 37 1 4 90 6 93 7.36e-30 112
Msa0705820 MtrunA17_Chr1g0182321 33.858 127 71 6 168 286 93 214 4.32e-11 61.2
Msa0705820 MtrunA17_Chr1g0182301 58.140 86 35 1 6 90 8 93 8.01e-30 112
Msa0705820 MtrunA17_Chr1g0182301 33.858 127 71 6 168 286 93 214 8.27e-11 60.5
Msa0705820 MtrunA17_Chr1g0212521 60.241 83 32 1 9 90 11 93 8.96e-30 112
Msa0705820 MtrunA17_Chr4g0030911 60.241 83 32 1 9 90 11 93 1.01e-29 112
Msa0705820 MtrunA17_Chr7g0263071 60.241 83 32 1 9 90 11 93 1.66e-29 111
Msa0705820 MtrunA17_Chr2g0276941 54.651 86 38 1 6 90 8 93 2.01e-28 108
Msa0705820 MtrunA17_Chr2g0319951 57.831 83 34 1 9 90 9 91 6.01e-28 107
Msa0705820 MtrunA17_Chr2g0327911 54.023 87 39 1 5 90 7 93 1.23e-27 106
Msa0705820 MtrunA17_Chr2g0327911 41.250 80 45 2 168 246 93 171 6.06e-11 60.8
Msa0705820 MtrunA17_Chr3g0112681 55.422 83 36 1 9 90 8 90 1.52e-27 106
Msa0705820 MtrunA17_Chr4g0032201 54.762 84 37 1 8 90 7 90 2.67e-27 105
Msa0705820 MtrunA17_Chr8g0392931 58.333 84 34 1 8 90 9 92 8.98e-27 104
Msa0705820 MtrunA17_Chr2g0325091 57.143 84 35 1 8 90 6 89 9.79e-27 103
Msa0705820 MtrunA17_Chr6g0482721 58.333 84 34 1 8 90 7 90 1.01e-26 103
Msa0705820 MtrunA17_Chr3g0123821 53.488 86 39 1 6 90 9 94 3.21e-26 102
Msa0705820 MtrunA17_Chr1g0189361 45.133 113 48 2 11 109 12 124 4.10e-26 102
Msa0705820 MtrunA17_Chr7g0234201 58.750 80 32 1 12 90 12 91 5.22e-26 102
Msa0705820 MtrunA17_Chr2g0315551 53.012 83 38 1 9 90 9 91 5.52e-26 102
Msa0705820 MtrunA17_Chr4g0046261 49.451 91 45 1 1 90 1 91 1.64e-25 100
Msa0705820 MtrunA17_Chr2g0283131 49.451 91 45 1 1 90 1 91 1.99e-25 100
Msa0705820 MtrunA17_Chr4g0040781 49.451 91 45 1 1 90 1 91 2.46e-25 100
Msa0705820 MtrunA17_Chr8g0346421 52.941 85 39 1 7 90 20 104 4.27e-25 100
Msa0705820 MtrunA17_Chr1g0174281 52.941 85 39 1 7 90 9 93 5.42e-25 99.8
Msa0705820 MtrunA17_Chr7g0275991 53.571 84 38 1 8 90 15 98 8.62e-25 99.4
Msa0705820 MtrunA17_Chr8g0381841 51.163 86 41 1 6 90 10 95 9.53e-25 99.0
Msa0705820 MtrunA17_Chr6g0458081 54.878 82 36 1 10 90 3 84 2.08e-24 97.8
Msa0705820 MtrunA17_Chr6g0458081 42.529 87 48 2 168 254 84 168 2.76e-14 70.5
Msa0705820 MtrunA17_Chr2g0290891 54.878 82 36 1 10 90 3 84 7.46e-24 96.3
Msa0705820 MtrunA17_Chr2g0290891 34.351 131 70 5 168 286 84 210 2.46e-13 67.8
Msa0705820 MtrunA17_Chr7g0231131 53.012 83 38 1 9 90 11 93 2.05e-23 95.5
Msa0705820 MtrunA17_Chr8g0351861 56.098 82 35 1 10 90 13 94 2.38e-23 95.5
Msa0705820 MtrunA17_Chr7g0214241 53.750 80 36 1 12 90 14 93 5.03e-23 94.4
Msa0705820 MtrunA17_Chr8g0349681 55.000 80 35 1 12 90 383 462 8.12e-23 98.2
Msa0705820 MtrunA17_Chr8g0349681 51.250 80 38 1 12 90 12 91 1.53e-18 85.5
Msa0705820 MtrunA17_Chr8g0349681 54.667 75 33 1 12 85 174 248 2.62e-18 84.7
Msa0705820 MtrunA17_Chr8g0349681 31.111 180 106 6 71 246 164 329 9.13e-14 71.2
Msa0705820 MtrunA17_Chr8g0349681 33.333 123 76 3 168 286 462 582 8.31e-13 68.6
Msa0705820 MtrunA17_Chr8g0349681 39.024 82 48 2 168 249 91 170 1.18e-12 67.8
Msa0705820 MtrunA17_Chr8g0349711 53.750 80 36 1 12 90 5 84 3.42e-22 92.0
Msa0705820 MtrunA17_Chr8g0349711 43.038 79 43 2 168 246 84 160 1.24e-14 71.2
Msa0705820 MtrunA17_Chr4g0055081 49.296 71 35 1 14 83 10 80 2.15e-16 75.9
Msa0705820 MtrunA17_Chr5g0400131 49.296 71 35 1 14 83 10 80 2.19e-16 75.9
Msa0705820 MtrunA17_Chr2g0331691 44.304 79 43 1 13 90 10 88 3.69e-16 75.5
Msa0705820 MtrunA17_Chr8g0382221 49.296 71 35 1 14 83 10 80 6.90e-16 74.7
Msa0705820 MtrunA17_Chr3g0123971 49.296 71 35 1 14 83 10 80 7.96e-16 74.3
Msa0705820 MtrunA17_Chr4g0034871 44.304 79 43 1 13 90 8 86 1.18e-15 73.9
Msa0705820 MtrunA17_Chr4g0004661 44.304 79 43 1 13 90 64 142 1.81e-15 74.7
Msa0705820 MtrunA17_Chr8g0383261 41.758 91 51 2 1 90 1 90 1.95e-15 73.6
Msa0705820 MtrunA17_Chr1g0201121 42.500 80 45 1 13 91 8 87 2.15e-15 73.2
Msa0705820 MtrunA17_Chr5g0433561 45.455 77 41 1 15 90 12 88 4.43e-15 72.4
Msa0705820 MtrunA17_Chr5g0433561 31.646 158 91 6 129 286 59 199 8.98e-13 65.9
Msa0705820 MtrunA17_Chr3g0109721 45.455 77 41 1 15 90 12 88 4.90e-15 72.0
Msa0705820 MtrunA17_Chr6g0466301 43.038 79 44 1 13 90 8 86 4.93e-15 72.4
Msa0705820 MtrunA17_Chr6g0465491 43.038 79 44 1 13 90 8 86 4.93e-15 72.4
Msa0705820 MtrunA17_Chr8g0390561 42.857 77 43 1 15 90 11 87 2.27e-14 70.1
Msa0705820 MtrunA17_Chr1g0199331 47.945 73 36 2 15 85 33 105 6.46e-14 68.9
Msa0705820 MtrunA17_Chr1g0199331 33.824 136 77 5 124 259 76 198 3.01e-13 67.0
Msa0705820 MtrunA17_Chr8g0371501 39.286 84 49 2 14 96 7 89 9.47e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705820 AT1G43890.3 83.486 109 18 0 168 276 90 198 1.03e-62 197
Msa0705820 AT1G43890.3 87.778 90 11 0 1 90 1 90 1.85e-48 160
Msa0705820 AT1G43890.1 83.486 109 18 0 168 276 90 198 1.03e-62 197
Msa0705820 AT1G43890.1 87.778 90 11 0 1 90 1 90 1.85e-48 160
Msa0705820 AT1G43890.2 83.486 109 18 0 168 276 90 198 1.03e-62 197
Msa0705820 AT1G43890.2 87.778 90 11 0 1 90 1 90 1.85e-48 160
Msa0705820 AT5G03530.2 63.333 120 43 1 168 286 90 209 5.64e-54 174
Msa0705820 AT5G03530.2 73.333 90 24 0 1 90 1 90 1.31e-40 140
Msa0705820 AT5G03530.1 63.333 120 43 1 168 286 90 209 5.64e-54 174
Msa0705820 AT5G03530.1 73.333 90 24 0 1 90 1 90 1.31e-40 140
Msa0705820 AT3G09910.3 58.824 119 44 1 168 286 90 203 8.03e-45 151
Msa0705820 AT3G09910.3 70.000 90 27 0 1 90 1 90 8.35e-33 120
Msa0705820 AT3G09910.1 58.824 119 44 1 168 286 90 203 8.03e-45 151
Msa0705820 AT3G09910.1 70.000 90 27 0 1 90 1 90 8.35e-33 120
Msa0705820 AT3G09910.2 58.824 119 44 1 168 286 90 203 8.03e-45 151
Msa0705820 AT3G09910.2 70.000 90 27 0 1 90 1 90 8.35e-33 120
Msa0705820 AT3G09910.5 64.516 93 30 1 168 257 90 182 9.75e-37 130
Msa0705820 AT3G09910.5 70.000 90 27 0 1 90 1 90 7.12e-33 120
Msa0705820 AT3G09910.4 64.516 93 30 1 168 257 90 182 9.75e-37 130
Msa0705820 AT3G09910.4 70.000 90 27 0 1 90 1 90 7.12e-33 120
Msa0705820 AT5G47200.1 69.880 83 24 1 9 90 4 86 1.86e-33 121
Msa0705820 AT5G47200.1 33.613 119 75 3 168 286 86 200 1.29e-13 68.6
Msa0705820 AT4G17530.1 69.880 83 24 1 9 90 4 86 2.30e-33 121
Msa0705820 AT4G17530.1 33.613 119 75 3 168 286 86 200 2.16e-13 68.2
Msa0705820 AT1G02130.1 68.675 83 25 1 9 90 4 86 5.82e-33 120
Msa0705820 AT1G02130.1 33.613 119 76 3 168 286 86 201 7.79e-13 66.6
Msa0705820 AT3G11730.1 67.470 83 26 1 9 90 4 86 5.69e-32 117
Msa0705820 AT3G11730.1 33.333 120 77 3 168 286 86 203 2.45e-11 62.4
Msa0705820 AT5G59840.1 60.241 83 32 1 9 90 11 93 1.50e-29 112
Msa0705820 AT3G46060.2 60.241 83 32 1 9 90 11 93 1.68e-29 111
Msa0705820 AT3G46060.3 60.241 83 32 1 9 90 11 93 1.68e-29 111
Msa0705820 AT3G46060.1 60.241 83 32 1 9 90 11 93 1.68e-29 111
Msa0705820 AT3G53610.2 60.241 83 32 1 9 90 11 93 1.78e-29 111
Msa0705820 AT3G53610.3 60.241 83 32 1 9 90 11 93 1.78e-29 111
Msa0705820 AT3G53610.1 60.241 83 32 1 9 90 11 93 1.78e-29 111
Msa0705820 AT1G06400.1 54.945 91 40 1 1 90 1 91 1.28e-28 109
Msa0705820 AT5G03520.1 57.955 88 36 1 4 90 6 93 1.52e-28 109
Msa0705820 AT3G09900.1 57.955 88 36 1 4 90 6 93 1.89e-28 108
Msa0705820 AT3G09900.1 36.000 125 72 5 168 286 93 215 6.37e-11 61.2
Msa0705820 AT3G46830.1 55.952 84 36 1 8 90 7 90 2.17e-27 106
Msa0705820 AT3G46830.2 55.952 84 36 1 8 90 7 90 2.17e-27 106
Msa0705820 AT1G18200.1 57.143 91 38 1 1 90 1 91 2.24e-27 106
Msa0705820 AT3G07410.1 60.494 81 31 1 11 90 10 90 3.27e-27 105
Msa0705820 AT1G09630.1 54.762 84 37 1 8 90 7 90 3.99e-27 105
Msa0705820 AT1G07410.1 54.762 84 37 1 8 90 7 90 4.26e-27 105
Msa0705820 AT5G59150.1 52.941 85 39 1 7 90 6 90 8.13e-27 104
Msa0705820 AT1G73640.1 54.945 91 40 1 1 90 1 91 1.51e-26 104
Msa0705820 AT1G16920.1 52.941 85 39 1 7 90 7 91 1.82e-26 103
Msa0705820 AT2G33870.1 50.549 91 44 1 1 90 1 91 4.04e-26 102
Msa0705820 AT2G33870.1 33.594 128 74 3 168 286 91 216 9.20e-11 60.8
Msa0705820 AT5G45750.1 54.217 83 37 1 9 90 9 91 4.90e-26 102
Msa0705820 AT5G60860.1 50.549 91 44 1 1 90 1 91 6.72e-26 102
Msa0705820 AT4G39990.1 56.790 81 34 1 11 90 15 95 1.09e-25 102
Msa0705820 AT4G39990.1 31.008 129 77 5 168 286 95 221 7.21e-11 61.2
Msa0705820 AT5G47520.1 56.790 81 34 1 11 90 12 92 1.77e-25 101
Msa0705820 AT4G35860.1 56.098 82 35 1 10 90 3 84 2.01e-25 100
Msa0705820 AT4G35860.1 43.678 87 47 2 168 254 84 168 3.25e-16 75.9
Msa0705820 AT1G28550.1 50.549 91 44 1 1 90 1 91 2.02e-25 100
Msa0705820 AT4G18430.1 50.549 91 44 1 1 90 1 91 3.50e-25 100
Msa0705820 AT2G31680.1 55.556 81 35 1 11 90 10 90 4.90e-25 100
Msa0705820 AT4G18800.1 53.012 83 38 1 9 90 9 91 5.17e-25 99.8
Msa0705820 AT1G05810.1 52.809 89 41 1 3 90 2 90 6.76e-25 99.8
Msa0705820 AT1G05810.1 33.594 128 74 4 168 286 90 215 5.08e-12 64.3
Msa0705820 AT3G12160.1 53.571 84 38 1 8 90 10 93 1.01e-24 99.4
Msa0705820 AT1G01200.1 53.571 84 38 1 8 90 23 106 1.20e-24 99.4
Msa0705820 AT5G65270.1 52.381 84 39 1 8 90 12 95 1.28e-24 99.0
Msa0705820 AT1G05810.2 52.809 89 41 1 3 90 48 136 1.31e-24 100
Msa0705820 AT1G05810.2 33.594 128 74 4 168 286 136 261 1.01e-11 64.3
Msa0705820 AT2G43130.1 54.321 81 36 1 11 90 10 90 1.66e-24 98.6
Msa0705820 AT5G47960.1 52.381 84 39 1 8 90 10 93 2.52e-24 98.2
Msa0705820 AT3G15060.1 47.253 91 47 1 1 90 1 91 3.15e-24 97.8
Msa0705820 AT4G17170.1 54.878 82 36 1 10 90 3 84 1.04e-23 96.3
Msa0705820 AT4G17170.1 36.434 129 70 5 168 286 84 210 2.14e-14 70.9
Msa0705820 AT4G17160.1 54.878 82 36 1 10 90 3 84 5.89e-23 94.0
Msa0705820 AT4G17160.1 38.095 126 66 5 168 286 84 204 1.22e-16 77.0
Msa0705820 AT4G39890.1 48.718 78 39 1 14 90 10 87 1.65e-17 79.7
Msa0705820 AT4G39890.1 34.906 106 66 2 168 271 87 191 2.62e-11 62.4
Msa0705820 AT4G35860.2 43.678 87 47 2 168 254 38 122 1.14e-16 76.3
Msa0705820 AT5G45130.1 43.820 89 47 2 3 90 2 88 1.28e-16 77.0
Msa0705820 AT4G19640.2 46.753 77 40 1 15 90 12 88 1.77e-16 75.1
Msa0705820 AT2G44610.1 49.296 71 35 1 14 83 10 80 2.62e-16 76.3
Msa0705820 AT4G19640.3 46.753 77 40 1 15 90 12 88 2.86e-16 75.9
Msa0705820 AT4G19640.1 46.753 77 40 1 15 90 12 88 4.26e-16 75.5
Msa0705820 AT3G16100.1 45.570 79 42 1 13 90 8 86 4.49e-16 75.5
Msa0705820 AT1G52280.1 45.570 79 42 1 13 90 8 86 5.49e-16 75.5
Msa0705820 AT2G21880.1 44.304 79 43 1 13 90 9 87 5.94e-16 75.5
Msa0705820 AT5G39620.1 46.154 78 41 1 14 90 6 83 8.00e-16 74.7
Msa0705820 AT2G22290.1 49.296 71 35 1 14 83 10 80 1.07e-15 74.3
Msa0705820 AT5G10260.1 49.296 71 35 1 14 83 10 80 1.21e-15 74.3
Msa0705820 AT3G18820.1 44.304 79 43 1 13 90 8 86 1.29e-15 74.3
Msa0705820 AT1G49300.2 44.304 79 43 1 13 90 8 86 2.01e-15 73.6
Msa0705820 AT1G49300.1 44.304 79 43 1 13 90 8 86 2.01e-15 73.6
Msa0705820 AT1G22740.1 43.038 79 44 1 13 90 8 86 2.08e-15 73.6
Msa0705820 AT5G03520.2 60.000 50 20 0 41 90 34 83 5.52e-15 72.4
Msa0705820 AT4G09720.1 47.297 74 38 1 13 85 8 81 7.02e-15 72.4
Msa0705820 AT4G09720.4 47.297 74 38 1 13 85 8 81 7.35e-15 72.4
Msa0705820 AT4G09720.3 47.297 74 38 1 13 85 8 81 8.55e-15 72.4
Msa0705820 AT5G45130.2 45.333 75 40 1 17 90 22 96 8.61e-15 72.0
Msa0705820 AT3G54840.2 47.945 73 36 2 15 85 35 107 5.07e-14 69.7
Msa0705820 AT3G54840.1 47.945 73 36 2 15 85 35 107 7.24e-14 69.3
Msa0705820 AT3G54840.1 33.333 126 72 3 124 249 78 191 7.74e-12 63.5
Msa0705820 AT3G54840.3 47.222 72 36 2 16 85 2 73 1.83e-13 67.4
Msa0705820 AT3G54840.3 33.333 126 72 3 124 249 44 157 4.03e-12 63.5
Msa0705820 AT5G64990.1 46.479 71 37 1 14 83 8 78 2.26e-13 68.2
Msa0705820 AT5G55080.1 41.111 90 48 5 5 90 4 92 9.18e-12 63.9
Msa0705820 AT5G46025.1 38.636 88 53 1 168 255 22 108 1.65e-11 60.5
Msa0705820 AT5G64990.2 42.308 78 37 2 14 83 8 85 7.75e-11 60.8
Msa0705820 AT2G44690.1 39.286 84 49 2 14 96 19 101 8.40e-11 60.8

Find 81 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAATATTGCAGTCATTT+TGG 0.199457 4_4:+78614644 None:intergenic
TTACAAATCTCTCTGAAATA+TGG 0.215859 4_4:-78614468 Msa0705820:CDS
TAGCACTGCATTCAATAAAT+AGG 0.259648 4_4:+78613301 None:intergenic
CAGAAAATGGATTCGAGTTC+AGG 0.266933 4_4:-78616111 Msa0705820:exon
ATAGACTTTGCTAGGGAATA+TGG 0.277032 4_4:-78613327 Msa0705820:CDS
AAGCTTGCCATTTGGGATAC+TGG 0.289194 4_4:-78615831 Msa0705820:intron
AACACTCAGCACATCTTTAC+AGG 0.298474 4_4:+78612606 None:intergenic
TTGTTAATGATTGGGGATTC+TGG 0.299697 4_4:-78616060 Msa0705820:CDS
CCTTAGAGCCCTCGGCTAAA+AGG 0.308198 4_4:+78612940 None:intergenic
ATGTTAGAAATCCATGAAAA+TGG 0.308707 4_4:+78612791 None:intergenic
ATAATAAGATTTGAACATAT+TGG 0.312652 4_4:-78612533 Msa0705820:three_prime_UTR
AGTTTCTTTCTGATAGATAC+TGG 0.314153 4_4:-78616332 Msa0705820:five_prime_UTR
AAAGCTTAAGCTTGCCATTT+GGG 0.317285 4_4:-78615838 Msa0705820:CDS
GATCGCCGACCCACTAATAT+TGG 0.333031 4_4:-78616310 Msa0705820:five_prime_UTR
TGTTCAAATTGTTAATGATT+GGG 0.336729 4_4:-78616068 Msa0705820:CDS
ATGTTCAAATTGTTAATGAT+TGG 0.346507 4_4:-78616069 Msa0705820:CDS
AGTCTTGATTTGTTGAATAA+AGG 0.348413 4_4:+78614431 None:intergenic
AAAATACATGATTTGAATAC+TGG 0.350066 4_4:+78614840 None:intergenic
ACCAAATAATGCAGCTGGTC+AGG 0.352646 4_4:-78615448 Msa0705820:intron
ACAGTAAATAAATGTACTAT+TGG 0.357343 4_4:+78615330 None:intergenic
TGGTAAAGCAAATGCATGTT+AGG 0.369362 4_4:-78616355 Msa0705820:five_prime_UTR
AAAAGCTTAAGCTTGCCATT+TGG 0.383397 4_4:-78615839 Msa0705820:CDS
GAAGTTGGAACCTAGAAAAC+TGG 0.389416 4_4:+78612731 None:intergenic
GGATTTCTAACATAATTTGC+TGG 0.390362 4_4:-78612781 Msa0705820:three_prime_UTR
GGACTTTCTTTGTTAGTGAT+GGG 0.390599 4_4:-78614675 Msa0705820:CDS
TGGACTTTCTTTGTTAGTGA+TGG 0.392991 4_4:-78614676 Msa0705820:CDS
AAGAAAGAAACCAATATTAG+TGG 0.394278 4_4:+78616300 None:intergenic
TACAAATCTCTCTGAAATAT+GGG 0.405801 4_4:-78614467 Msa0705820:CDS
GATGATCTCTCTCCCACCAT+TGG 0.416793 4_4:-78615991 Msa0705820:intron
GACTGCATGAAGATGCTTGT+TGG 0.424153 4_4:-78614412 Msa0705820:CDS
CTCTTGTCATCTTAATCTGT+TGG 0.452730 4_4:-78612504 Msa0705820:three_prime_UTR
TGGATGCAATGCCCTTCTCT+TGG 0.453432 4_4:+78612811 None:intergenic
AAGAGGGAATAGACTTTGCT+AGG 0.457004 4_4:-78613335 Msa0705820:CDS
TAATAATCTGTAGGATTCAA+TGG 0.458814 4_4:-78614705 Msa0705820:intron
TTGTGGGAGGGGATGCTTGT+TGG 0.459665 4_4:-78616375 Msa0705820:five_prime_UTR
GGAACCTAGAAAACTGGAAA+TGG 0.464492 4_4:+78612737 None:intergenic
GTTCAAATTGTTAATGATTG+GGG 0.472425 4_4:-78616067 Msa0705820:CDS
CTACATATTCAAGACAAAAC+AGG 0.472563 4_4:+78612709 None:intergenic
TAATAGTCTGTAGGATTCAA+TGG 0.481798 4_4:-78614863 Msa0705820:intron
GGTCAAATATGTCACGATAG+AGG 0.481991 4_4:-78615865 Msa0705820:CDS
TTCAAGACAAAACAGGAAGT+TGG 0.484863 4_4:+78612716 None:intergenic
GACTTTCTTTGTTAGTGATG+GGG 0.491805 4_4:-78614674 Msa0705820:CDS
CTAAATATTAGAAACAGGCT+TGG 0.493825 4_4:+78612441 None:intergenic
TGTGTGACAGAATCAGAAAA+TGG 0.498954 4_4:-78616124 Msa0705820:intron
ATGATTGGGGATTCTGGTGT+TGG 0.503280 4_4:-78616054 Msa0705820:CDS
AGAAAATGGATTCGAGTTCA+GGG 0.507840 4_4:-78616110 Msa0705820:exon
TTGTATAGTACCCAATGAAA+AGG 0.512553 4_4:+78616234 None:intergenic
TAGAGTTGTGACAAAGAAAG+AGG 0.518206 4_4:-78613352 Msa0705820:CDS
GTCAAATATGTCACGATAGA+GGG 0.528925 4_4:-78615864 Msa0705820:CDS
TGTTGGAAACAAAGTAGATA+AGG 0.532932 4_4:-78614395 Msa0705820:intron
GTAGGATTCAATGGCCAAGT+TGG 0.538976 4_4:-78614696 Msa0705820:intron
AGAGTTGTGACAAAGAAAGA+GGG 0.538991 4_4:-78613351 Msa0705820:CDS
TCTTTCATCTAATAGTCTGT+AGG 0.546041 4_4:-78614872 Msa0705820:intron
TAGTACCCAATGAAAAGGAC+AGG 0.550018 4_4:+78616239 None:intergenic
TCTTACTACAGAGGGGCTCA+AGG 0.553803 4_4:-78615402 Msa0705820:CDS
GAATCACGGTCTCCAAGAGA+AGG 0.560488 4_4:-78612823 Msa0705820:three_prime_UTR
TGACAAGTTCTTACTACAGA+GGG 0.566870 4_4:-78615410 Msa0705820:CDS
GAAAGGAAACGAACCAATGG+TGG 0.568646 4_4:+78615978 None:intergenic
ATACATACCAGTATCCCAAA+TGG 0.569683 4_4:+78615824 None:intergenic
TCTCTCTGAAATATGGGCAA+AGG 0.571068 4_4:-78614461 Msa0705820:CDS
GAGCCCTCGGCTAAAAGGCT+AGG 0.572458 4_4:+78612945 None:intergenic
AGGGGCTCAAGGAATCATCA+TGG 0.573145 4_4:-78615391 Msa0705820:CDS
AGAGGGAATAGACTTTGCTA+GGG 0.577586 4_4:-78613334 Msa0705820:CDS
GCTTCAGCAACAACCGCTGG+TGG 0.578132 4_4:+78612872 None:intergenic
CATCTTTACAGGTCTTCACA+TGG 0.584085 4_4:+78612617 None:intergenic
AGAAAGAAACCAATATTAGT+GGG 0.587334 4_4:+78616301 None:intergenic
ATAGCTTCAGCAACAACCGC+TGG 0.588465 4_4:+78612869 None:intergenic
CTAACAAAGAAAGTCCAACT+TGG 0.594978 4_4:+78614682 None:intergenic
GGGGCTCAAGGAATCATCAT+GGG 0.609277 4_4:-78615390 Msa0705820:CDS
AGAAACCAATATTAGTGGGT+CGG 0.609461 4_4:+78616305 None:intergenic
AAGCAGCATTTCAAGAATCA+CGG 0.626294 4_4:-78612837 Msa0705820:three_prime_UTR
GAGATCATCAAAATCATCTG+AGG 0.628890 4_4:+78616005 None:intergenic
AATCACGGTCTCCAAGAGAA+GGG 0.631244 4_4:-78612822 Msa0705820:three_prime_UTR
GTACTATTGGAAGAATTGAG+AGG 0.635651 4_4:+78615343 None:intergenic
CAGTCTGATGCATCCACCAG+CGG 0.644893 4_4:-78612885 Msa0705820:CDS
AAAGGAAACGAACCAATGGT+GGG 0.650141 4_4:+78615979 None:intergenic
ATGTAGATGTAATATAGTGA+CGG 0.650213 4_4:-78612692 Msa0705820:three_prime_UTR
CTGGTGGATGCATCAGACTG+AGG 0.668797 4_4:+78612888 None:intergenic
CTGACAAGTTCTTACTACAG+AGG 0.693244 4_4:-78615411 Msa0705820:CDS
ATGCAGTTTATGACGTAACA+CGG 0.694182 4_4:-78614501 Msa0705820:intron
GACAAGTTCTTACTACAGAG+GGG 0.732850 4_4:-78615409 Msa0705820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACATTTTTGTATAATTGT+GGG - chr4_4:78612460-78612479 Msa0705820:three_prime_UTR 15.0%
!! AGATAGAATTAAATCAATTA+AGG + chr4_4:78612902-78612921 None:intergenic 15.0%
!!! TTATTTTGTAATCTTGTTAT+TGG - chr4_4:78613140-78613159 Msa0705820:intron 15.0%
!! TGTAAAATGTGAATTAATTT+AGG + chr4_4:78613755-78613774 None:intergenic 15.0%
!! TAATAATCAATATCATCATT+GGG + chr4_4:78613822-78613841 None:intergenic 15.0%
!!! TATTCAAATCATGTATTTTT+TGG - chr4_4:78614015-78614034 Msa0705820:intron 15.0%
!! ATTTGACATAAAATTCTTAA+AGG + chr4_4:78614486-78614505 None:intergenic 15.0%
!!! AAATTTATCCATGTATTTTT+TGG - chr4_4:78614564-78614583 Msa0705820:intron 15.0%
!! AAAAAATTGAATATCATTAG+AGG - chr4_4:78615077-78615096 Msa0705820:intron 15.0%
!! AAAAAATTGAATATCATTAG+AGG - chr4_4:78615077-78615096 Msa0705820:intron 15.0%
!!! AGTAAAAAAGAACATTTTTA+AGG - chr4_4:78615935-78615954 Msa0705820:intron 15.0%
!! ATAATAAGATTTGAACATAT+TGG - chr4_4:78616318-78616337 Msa0705820:five_prime_UTR 15.0%
!!! GAAACATTTTTGTATAATTG+TGG - chr4_4:78612459-78612478 Msa0705820:three_prime_UTR 20.0%
!! ATGTTCAAATTGTTAATGAT+TGG - chr4_4:78612782-78612801 Msa0705820:three_prime_UTR 20.0%
!! TGTTCAAATTGTTAATGATT+GGG - chr4_4:78612783-78612802 Msa0705820:three_prime_UTR 20.0%
!! TAAATCAATTAAGGAATGAA+AGG + chr4_4:78612893-78612912 None:intergenic 20.0%
!! TAAATAAAACTCCAATCTAA+TGG + chr4_4:78613123-78613142 None:intergenic 20.0%
!! TAATTATTGGTAAAAGTTGA+TGG + chr4_4:78613183-78613202 None:intergenic 20.0%
!! TACCAATAATTAATCTTTAG+CGG - chr4_4:78613191-78613210 Msa0705820:intron 20.0%
!!! GATATTGGTTTTTAGATTAA+AGG - chr4_4:78613300-78613319 Msa0705820:CDS 20.0%
!! AGTAGAATATCAATCAAATA+GGG + chr4_4:78613373-78613392 None:intergenic 20.0%
!! AAGTAGAATATCAATCAAAT+AGG + chr4_4:78613374-78613393 None:intergenic 20.0%
!! ACAGTAAATAAATGTACTAT+TGG + chr4_4:78613524-78613543 None:intergenic 20.0%
!!! AATTATTTGTTTTCTGAGTA+GGG - chr4_4:78613670-78613689 Msa0705820:intron 20.0%
!! CTAATAATCAATATCATCAT+TGG + chr4_4:78613823-78613842 None:intergenic 20.0%
!! AAAATACATGATTTGAATAC+TGG + chr4_4:78614014-78614033 None:intergenic 20.0%
!! TAAATTAAATACCACATGAA+AGG + chr4_4:78615248-78615267 None:intergenic 20.0%
!!! ATTTAATTTGTGATGAACAA+TGG - chr4_4:78615829-78615848 Msa0705820:intron 20.0%
!! ATTTTTGTATAATTGTGGGA+GGG - chr4_4:78612464-78612483 Msa0705820:three_prime_UTR 25.0%
! AGAAAGAAACCAATATTAGT+GGG + chr4_4:78612553-78612572 None:intergenic 25.0%
! AAGAAAGAAACCAATATTAG+TGG + chr4_4:78612554-78612573 None:intergenic 25.0%
! GTTCAAATTGTTAATGATTG+GGG - chr4_4:78612784-78612803 Msa0705820:three_prime_UTR 25.0%
! AATGGACAAGGATTTAAAAA+TGG + chr4_4:78613105-78613124 None:intergenic 25.0%
!!! TTTTGTAATCTTGTTATTGG+TGG - chr4_4:78613143-78613162 Msa0705820:intron 25.0%
! ACCAATAATTAATCTTTAGC+GGG - chr4_4:78613192-78613211 Msa0705820:intron 25.0%
! TTAAAGGCCAATTGTATATA+TGG - chr4_4:78613316-78613335 Msa0705820:CDS 25.0%
! TAAAGGCCAATTGTATATAT+GGG - chr4_4:78613317-78613336 Msa0705820:CDS 25.0%
! TATTGGGAAGACAATTTAAA+AGG + chr4_4:78613350-78613369 None:intergenic 25.0%
!! TATCAATCAAATAGGGTATT+GGG + chr4_4:78613366-78613385 None:intergenic 25.0%
! ATATCAATCAAATAGGGTAT+TGG + chr4_4:78613367-78613386 None:intergenic 25.0%
! ATATATTCAGTGGATAGAAT+AGG - chr4_4:78613578-78613597 Msa0705820:intron 25.0%
!!! GAATTATTTGTTTTCTGAGT+AGG - chr4_4:78613669-78613688 Msa0705820:intron 25.0%
!! CTTTAAACATCAAGTAGTTT+TGG + chr4_4:78613702-78613721 None:intergenic 25.0%
! TAGGTAAATATTTAACCATG+AGG + chr4_4:78613736-78613755 None:intergenic 25.0%
! TTGATATCATTCATATTGCT+TGG - chr4_4:78614065-78614084 Msa0705820:intron 25.0%
! TGATATCATTCATATTGCTT+GGG - chr4_4:78614066-78614085 Msa0705820:intron 25.0%
! TCTTTCATCTAATAATCTGT+AGG - chr4_4:78614137-78614156 Msa0705820:intron 25.0%
! TAATAATCTGTAGGATTCAA+TGG - chr4_4:78614146-78614165 Msa0705820:intron 25.0%
!! TACAAATATTGCAGTCATTT+TGG + chr4_4:78614210-78614229 None:intergenic 25.0%
! AACAAAATATAGAGGTAATC+AGG + chr4_4:78614320-78614339 None:intergenic 25.0%
!!! TTACAAATCTCTCTGAAATA+TGG - chr4_4:78614383-78614402 Msa0705820:intron 25.0%
!!! TACAAATCTCTCTGAAATAT+GGG - chr4_4:78614384-78614403 Msa0705820:intron 25.0%
!!! AGTCTTGATTTGTTGAATAA+AGG + chr4_4:78614423-78614442 None:intergenic 25.0%
!! AATGACATAGATTTTGAACA+AGG + chr4_4:78614594-78614613 None:intergenic 25.0%
!! ATTCAATCGTTAGCAAATTT+TGG - chr4_4:78614653-78614672 Msa0705820:CDS 25.0%
!!! TTGTTTTTCTAGTTTGCATT+TGG - chr4_4:78614893-78614912 Msa0705820:intron 25.0%
!! TCTTTTACATATGTAGAAGT+TGG - chr4_4:78614941-78614960 Msa0705820:intron 25.0%
! TTCCTAAAAGTTATCATTTC+CGG + chr4_4:78615055-78615074 None:intergenic 25.0%
!! TTTTTCTTGCCTTACATTAT+AGG - chr4_4:78615424-78615443 Msa0705820:CDS 25.0%
! ATGTCAAATCCTATAATGTA+AGG + chr4_4:78615436-78615455 None:intergenic 25.0%
!!! CTGATTTACTAACCTTATTT+TGG - chr4_4:78615641-78615660 Msa0705820:intron 25.0%
! ATGTTAGAAATCCATGAAAA+TGG + chr4_4:78616063-78616082 None:intergenic 25.0%
! ATGTAGATGTAATATAGTGA+CGG - chr4_4:78616159-78616178 Msa0705820:intron 25.0%
! CATTTTTGTATAATTGTGGG+AGG - chr4_4:78612463-78612482 Msa0705820:three_prime_UTR 30.0%
! TTTTTGTATAATTGTGGGAG+GGG - chr4_4:78612465-78612484 Msa0705820:three_prime_UTR 30.0%
AGTTTCTTTCTGATAGATAC+TGG - chr4_4:78612519-78612538 Msa0705820:three_prime_UTR 30.0%
TTGTATAGTACCCAATGAAA+AGG + chr4_4:78612620-78612639 None:intergenic 30.0%
!!! TATCACAGGTGTTGATTTTA+AGG - chr4_4:78612965-78612984 Msa0705820:intron 30.0%
TAAATCCTTGTCCATTAGAT+TGG - chr4_4:78613109-78613128 Msa0705820:intron 30.0%
CCAATAATTAATCTTTAGCG+GGG - chr4_4:78613193-78613212 Msa0705820:intron 30.0%
TACTTGGAACAATGTGATAT+TGG - chr4_4:78613285-78613304 Msa0705820:CDS 30.0%
AAAGGCCAATTGTATATATG+GGG - chr4_4:78613318-78613337 Msa0705820:CDS 30.0%
AAGAATTGAGAGGCAAAAAA+GGG + chr4_4:78613501-78613520 None:intergenic 30.0%
TCTATCCACTGAATATATCA+CGG + chr4_4:78613576-78613595 None:intergenic 30.0%
!! AGCTTCGATTTTGATAAGAA+TGG + chr4_4:78613862-78613881 None:intergenic 30.0%
TCGATATCATTCATATTGCT+TGG - chr4_4:78613907-78613926 Msa0705820:intron 30.0%
CGATATCATTCATATTGCTT+GGG - chr4_4:78613908-78613927 Msa0705820:intron 30.0%
TCTTTCATCTAATAGTCTGT+AGG - chr4_4:78613979-78613998 Msa0705820:intron 30.0%
TAATAGTCTGTAGGATTCAA+TGG - chr4_4:78613988-78614007 Msa0705820:intron 30.0%
GCAATCTGTTTAATGATCTT+TGG - chr4_4:78614236-78614255 Msa0705820:intron 30.0%
!! TGTTTAATGATCTTTGGAGT+TGG - chr4_4:78614242-78614261 Msa0705820:intron 30.0%
!! GTTTAATGATCTTTGGAGTT+GGG - chr4_4:78614243-78614262 Msa0705820:intron 30.0%
!!! GTTTTGGGAACAAAATATAG+AGG + chr4_4:78614328-78614347 None:intergenic 30.0%
! TCATAAACTGCATCAGTTTT+GGG + chr4_4:78614343-78614362 None:intergenic 30.0%
TGTTGGAAACAAAGTAGATA+AGG - chr4_4:78614456-78614475 Msa0705820:CDS 30.0%
ACTGACATGATTTGAATACA+AGG + chr4_4:78614526-78614545 None:intergenic 30.0%
TTTCTTCCTTATAGAGAACT+TGG - chr4_4:78614629-78614648 Msa0705820:intron 30.0%
TGAATTCCAAGTTCTCTATA+AGG + chr4_4:78614638-78614657 None:intergenic 30.0%
TTGACACTTCTGCTTATTTA+AGG - chr4_4:78614798-78614817 Msa0705820:intron 30.0%
!! CTGCTTATTTAAGGTGTTTT+TGG - chr4_4:78614807-78614826 Msa0705820:intron 30.0%
TTAATTGCATAACAGATGGA+CGG + chr4_4:78614872-78614891 None:intergenic 30.0%
CAACTTAATTGCATAACAGA+TGG + chr4_4:78614876-78614895 None:intergenic 30.0%
TAAAAGTTATCATTTCCGGA+GGG + chr4_4:78615051-78615070 None:intergenic 30.0%
TAAACTTTATCATTTCCGGA+GGG + chr4_4:78615144-78615163 None:intergenic 30.0%
TCCGGAAATGATAAAGTTTA+GGG - chr4_4:78615144-78615163 Msa0705820:intron 30.0%
TCCCTAAACTTTATCATTTC+CGG + chr4_4:78615148-78615167 None:intergenic 30.0%
GTAAAAGAAGACAATGAAGA+AGG - chr4_4:78615211-78615230 Msa0705820:intron 30.0%
CATACAAATCATGAATTGCA+TGG - chr4_4:78615272-78615291 Msa0705820:intron 30.0%
! CTGTTATCACATTTGTTTCT+AGG - chr4_4:78615360-78615379 Msa0705820:CDS 30.0%
TTATAGGATTTGACATGTGT+TGG - chr4_4:78615440-78615459 Msa0705820:intron 30.0%
! ATGTTTGTGCTTGTATTTTC+AGG - chr4_4:78615469-78615488 Msa0705820:intron 30.0%
TAGCACTGCATTCAATAAAT+AGG + chr4_4:78615553-78615572 None:intergenic 30.0%
TGTGAAACGTAAATTCTCTT+TGG - chr4_4:78615674-78615693 Msa0705820:intron 30.0%
GTGAAACGTAAATTCTCTTT+GGG - chr4_4:78615675-78615694 Msa0705820:intron 30.0%
! TGTTTTTCACACAAGTTCAA+TGG - chr4_4:78615768-78615787 Msa0705820:intron 30.0%
!!! ACTGGGGATTTTTTTTTCTT+TGG - chr4_4:78615861-78615880 Msa0705820:CDS 30.0%
! GGATTTCTAACATAATTTGC+TGG - chr4_4:78616070-78616089 Msa0705820:CDS 30.0%
CTACATATTCAAGACAAAAC+AGG + chr4_4:78616145-78616164 None:intergenic 30.0%
TGGTAAAGCAAATGCATGTT+AGG - chr4_4:78612496-78612515 Msa0705820:three_prime_UTR 35.0%
! AGAAACCAATATTAGTGGGT+CGG + chr4_4:78612549-78612568 None:intergenic 35.0%
! TTCATTCCTGTCCTTTTCAT+TGG - chr4_4:78612606-78612625 Msa0705820:three_prime_UTR 35.0%
! TCATTCCTGTCCTTTTCATT+GGG - chr4_4:78612607-78612626 Msa0705820:three_prime_UTR 35.0%
TGTGTGACAGAATCAGAAAA+TGG - chr4_4:78612727-78612746 Msa0705820:three_prime_UTR 35.0%
! AGAAAATGGATTCGAGTTCA+GGG - chr4_4:78612741-78612760 Msa0705820:three_prime_UTR 35.0%
TTGTTAATGATTGGGGATTC+TGG - chr4_4:78612791-78612810 Msa0705820:three_prime_UTR 35.0%
GAGATCATCAAAATCATCTG+AGG + chr4_4:78612849-78612868 None:intergenic 35.0%
AATGAAAGGAAACGAACCAA+TGG + chr4_4:78612879-78612898 None:intergenic 35.0%
GTCAAATATGTCACGATAGA+GGG - chr4_4:78612987-78613006 Msa0705820:intron 35.0%
AAAAGCTTAAGCTTGCCATT+TGG - chr4_4:78613012-78613031 Msa0705820:intron 35.0%
! AAAGCTTAAGCTTGCCATTT+GGG - chr4_4:78613013-78613032 Msa0705820:intron 35.0%
ATACATACCAGTATCCCAAA+TGG + chr4_4:78613030-78613049 None:intergenic 35.0%
! AAACTCCAATCTAATGGACA+AGG + chr4_4:78613117-78613136 None:intergenic 35.0%
CCCCGCTAAAGATTAATTAT+TGG + chr4_4:78613196-78613215 None:intergenic 35.0%
ACGAAAAAATGTGGTTGCAA+GGG + chr4_4:78613223-78613242 None:intergenic 35.0%
TTTCAAGCCACGAAAAAATG+TGG + chr4_4:78613232-78613251 None:intergenic 35.0%
TTGAAATGAGACCATGCTTA+AGG - chr4_4:78613246-78613265 Msa0705820:intron 35.0%
TGAAATGAGACCATGCTTAA+GGG - chr4_4:78613247-78613266 Msa0705820:intron 35.0%
!! GATCCAAATCCACTTTTACT+TGG - chr4_4:78613269-78613288 Msa0705820:CDS 35.0%
! GTTCCAAGTAAAAGTGGATT+TGG + chr4_4:78613275-78613294 None:intergenic 35.0%
CACATTGTTCCAAGTAAAAG+TGG + chr4_4:78613281-78613300 None:intergenic 35.0%
AAGGCCAATTGTATATATGG+GGG - chr4_4:78613319-78613338 Msa0705820:CDS 35.0%
! GTTTTACCAAATAATGCAGC+TGG - chr4_4:78613398-78613417 Msa0705820:intron 35.0%
TGACAAGTTCTTACTACAGA+GGG - chr4_4:78613441-78613460 Msa0705820:intron 35.0%
GAAGAATTGAGAGGCAAAAA+AGG + chr4_4:78613502-78613521 None:intergenic 35.0%
GTACTATTGGAAGAATTGAG+AGG + chr4_4:78613511-78613530 None:intergenic 35.0%
AAGAATACAGCAATCCAGAT+TGG + chr4_4:78613607-78613626 None:intergenic 35.0%
!!! AAAGCTTTTTTGTTGCCTCA+TGG - chr4_4:78613718-78613737 Msa0705820:intron 35.0%
! CTAACAAATCAGTTGTTTCC+AGG + chr4_4:78613951-78613970 None:intergenic 35.0%
! CTAACAAATCAGTTGTTTCC+AGG + chr4_4:78613951-78613970 None:intergenic 35.0%
CTAACAAAGAAAGTCCAACT+TGG + chr4_4:78614172-78614191 None:intergenic 35.0%
TGGACTTTCTTTGTTAGTGA+TGG - chr4_4:78614175-78614194 Msa0705820:intron 35.0%
GGACTTTCTTTGTTAGTGAT+GGG - chr4_4:78614176-78614195 Msa0705820:intron 35.0%
GACTTTCTTTGTTAGTGATG+GGG - chr4_4:78614177-78614196 Msa0705820:intron 35.0%
!! ATATTGCAGTCATTTTGGCA+GGG + chr4_4:78614205-78614224 None:intergenic 35.0%
!! AATATTGCAGTCATTTTGGC+AGG + chr4_4:78614206-78614225 None:intergenic 35.0%
CACGCATCAACTTAAAGAAA+AGG + chr4_4:78614275-78614294 None:intergenic 35.0%
! GCGTGTTGAAAACTTAGTTT+TGG - chr4_4:78614290-78614309 Msa0705820:intron 35.0%
! GTCATAAACTGCATCAGTTT+TGG + chr4_4:78614344-78614363 None:intergenic 35.0%
ATGCAGTTTATGACGTAACA+CGG - chr4_4:78614350-78614369 Msa0705820:intron 35.0%
AAGGCAAGCCAAAAAATACA+TGG + chr4_4:78614575-78614594 None:intergenic 35.0%
TAATTGCATAACAGATGGAC+GGG + chr4_4:78614871-78614890 None:intergenic 35.0%
!! TTGTTTTGTGCTTTTGCTTC+TGG - chr4_4:78615003-78615022 Msa0705820:intron 35.0%
CTAAAAGTTATCATTTCCGG+AGG + chr4_4:78615052-78615071 None:intergenic 35.0%
! CTCCGGAAATGATAACTTTT+AGG - chr4_4:78615050-78615069 Msa0705820:intron 35.0%
CTAAACTTTATCATTTCCGG+AGG + chr4_4:78615145-78615164 None:intergenic 35.0%
CTCCGGAAATGATAAAGTTT+AGG - chr4_4:78615143-78615162 Msa0705820:intron 35.0%
TTTGTTTGCATCCTTTCATG+TGG - chr4_4:78615234-78615253 Msa0705820:intron 35.0%
TGTAAAACTGAGAGTCCAAA+TGG + chr4_4:78615301-78615320 None:intergenic 35.0%
!! TTTGTGCTTGTATTTTCAGG+AGG - chr4_4:78615472-78615491 Msa0705820:intron 35.0%
!! TTGTGCTTGTATTTTCAGGA+GGG - chr4_4:78615473-78615492 Msa0705820:intron 35.0%
TAGAGTTGTGACAAAGAAAG+AGG - chr4_4:78615499-78615518 Msa0705820:intron 35.0%
AGAGTTGTGACAAAGAAAGA+GGG - chr4_4:78615500-78615519 Msa0705820:intron 35.0%
ATAGACTTTGCTAGGGAATA+TGG - chr4_4:78615524-78615543 Msa0705820:intron 35.0%
!!! CTTTGAAGAGCTTGTTTTGA+AGG - chr4_4:78615598-78615617 Msa0705820:intron 35.0%
!!! CTAACCTTATTTTGGTCAGT+AGG - chr4_4:78615649-78615668 Msa0705820:intron 35.0%
TTTCCACCTTCTAATATGCA+TGG + chr4_4:78615706-78615725 None:intergenic 35.0%
GTTCAATGGCTGACAATAAA+TGG - chr4_4:78615782-78615801 Msa0705820:intron 35.0%
!!! TTTTTTCTTTGGTTGCATGC+AGG - chr4_4:78615872-78615891 Msa0705820:CDS 35.0%
AAGCAGCATTTCAAGAATCA+CGG - chr4_4:78616014-78616033 Msa0705820:CDS 35.0%
TTCAAGACAAAACAGGAAGT+TGG + chr4_4:78616138-78616157 None:intergenic 35.0%
CTCTTGTCATCTTAATCTGT+TGG - chr4_4:78616347-78616366 Msa0705820:five_prime_UTR 35.0%
TAGTACCCAATGAAAAGGAC+AGG + chr4_4:78612615-78612634 None:intergenic 40.0%
! CAGAAAATGGATTCGAGTTC+AGG - chr4_4:78612740-78612759 Msa0705820:three_prime_UTR 40.0%
AAAGGAAACGAACCAATGGT+GGG + chr4_4:78612875-78612894 None:intergenic 40.0%
CAACACCTGTGATAGATAGT+AGG + chr4_4:78612959-78612978 None:intergenic 40.0%
GGTCAAATATGTCACGATAG+AGG - chr4_4:78612986-78613005 Msa0705820:intron 40.0%
CACGAAAAAATGTGGTTGCA+AGG + chr4_4:78613224-78613243 None:intergenic 40.0%
!!! CTTGCAACCACATTTTTTCG+TGG - chr4_4:78613222-78613241 Msa0705820:intron 40.0%
CTCTCCCCCATATATACAAT+TGG + chr4_4:78613326-78613345 None:intergenic 40.0%
CTGACAAGTTCTTACTACAG+AGG - chr4_4:78613440-78613459 Msa0705820:intron 40.0%
GACAAGTTCTTACTACAGAG+GGG - chr4_4:78613442-78613461 Msa0705820:intron 40.0%
AGAGGCAAAAAAGGGAGAAT+AGG + chr4_4:78613493-78613512 None:intergenic 40.0%
GATGACCGTGATATATTCAG+TGG - chr4_4:78613568-78613587 Msa0705820:intron 40.0%
GATAGAATAGGATGCCAATC+TGG - chr4_4:78613590-78613609 Msa0705820:intron 40.0%
! TTGTTTTCTGAGTAGGGCTA+AGG - chr4_4:78613676-78613695 Msa0705820:intron 40.0%
!! TCATTTTTCTGACCTCTGCT+GGG - chr4_4:78613784-78613803 Msa0705820:intron 40.0%
GAATATCAGCCAGTATATCC+TGG - chr4_4:78613930-78613949 Msa0705820:intron 40.0%
! CAGTTGTTTCCAGGATATAC+TGG + chr4_4:78613942-78613961 None:intergenic 40.0%
GAATATCAGCCAGTATATCC+TGG - chr4_4:78613930-78613949 Msa0705820:intron 40.0%
! CAGTTGTTTCCAGGATATAC+TGG + chr4_4:78613942-78613961 None:intergenic 40.0%
!! TCTCTCTGAAATATGGGCAA+AGG - chr4_4:78614390-78614409 Msa0705820:intron 40.0%
!! GGAAGAAAACTTTAAAGCGC+TGG + chr4_4:78614617-78614636 None:intergenic 40.0%
CGATCTGCGATTTACTTAGA+AGG + chr4_4:78614699-78614718 None:intergenic 40.0%
TCTAAGTAAATCGCAGATCG+CGG - chr4_4:78614699-78614718 Msa0705820:intron 40.0%
!! TTTAGAGAGTACTGCAGAGA+AGG - chr4_4:78614760-78614779 Msa0705820:intron 40.0%
TGAATTGCATGGACTCCATT+TGG - chr4_4:78615283-78615302 Msa0705820:intron 40.0%
AAGAGGGAATAGACTTTGCT+AGG - chr4_4:78615516-78615535 Msa0705820:intron 40.0%
AGAGGGAATAGACTTTGCTA+GGG - chr4_4:78615517-78615536 Msa0705820:intron 40.0%
CACACCTACTGACCAAAATA+AGG + chr4_4:78615656-78615675 None:intergenic 40.0%
GTGCAACCATGCATATTAGA+AGG - chr4_4:78615697-78615716 Msa0705820:intron 40.0%
CAACCATGCATATTAGAAGG+TGG - chr4_4:78615700-78615719 Msa0705820:intron 40.0%
AAGATGAGCCTGTGCAATTT+CGG - chr4_4:78615737-78615756 Msa0705820:intron 40.0%
TTGTGATGAACAATGGCTGA+TGG - chr4_4:78615836-78615855 Msa0705820:CDS 40.0%
! GAAGCCATTTCCAGTTTTCT+AGG - chr4_4:78616110-78616129 Msa0705820:exon 40.0%
GGAACCTAGAAAACTGGAAA+TGG + chr4_4:78616117-78616136 None:intergenic 40.0%
GAAGTTGGAACCTAGAAAAC+TGG + chr4_4:78616123-78616142 None:intergenic 40.0%
CATCTTTACAGGTCTTCACA+TGG + chr4_4:78616237-78616256 None:intergenic 40.0%
AACACTCAGCACATCTTTAC+AGG + chr4_4:78616248-78616267 None:intergenic 40.0%
ATGATTGGGGATTCTGGTGT+TGG - chr4_4:78612797-78612816 Msa0705820:three_prime_UTR 45.0%
GAAAGGAAACGAACCAATGG+TGG + chr4_4:78612876-78612895 None:intergenic 45.0%
CGTCTCCTACTATCTATCAC+AGG - chr4_4:78612951-78612970 Msa0705820:CDS 45.0%
! AAGCTTGCCATTTGGGATAC+TGG - chr4_4:78613020-78613039 Msa0705820:intron 45.0%
GGATTTGGATCCCTTAAGCA+TGG + chr4_4:78613260-78613279 None:intergenic 45.0%
ACCAAATAATGCAGCTGGTC+AGG - chr4_4:78613403-78613422 Msa0705820:intron 45.0%
TCCTGACCAGCTGCATTATT+TGG + chr4_4:78613407-78613426 None:intergenic 45.0%
ATCACGGTCATCAATACTCG+AGG + chr4_4:78613560-78613579 None:intergenic 45.0%
!!! GTCATTTTTCTGACCTCTGC+TGG - chr4_4:78613783-78613802 Msa0705820:intron 45.0%
GTAGGATTCAATGGCCAAGT+TGG - chr4_4:78614155-78614174 Msa0705820:intron 45.0%
GACTGCATGAAGATGCTTGT+TGG - chr4_4:78614439-78614458 Msa0705820:CDS 45.0%
AAACTCCTGCAGAAACATGC+TGG + chr4_4:78614922-78614941 None:intergenic 45.0%
AGCATGATTGCTTATCCCTC+CGG - chr4_4:78615033-78615052 Msa0705820:intron 45.0%
!!! GAGGAAAGTGCTTTTGCTTC+TGG - chr4_4:78615096-78615115 Msa0705820:intron 45.0%
AGCATGATTGCTTATCCCTC+CGG - chr4_4:78615033-78615052 Msa0705820:intron 45.0%
AGATGAGCCTGTGCAATTTC+GGG - chr4_4:78615738-78615757 Msa0705820:intron 45.0%
! AACAATGGCTGATGGTGACT+GGG - chr4_4:78615844-78615863 Msa0705820:CDS 45.0%
! TTTTACTCCCTTAGAGCCCT+CGG + chr4_4:78615922-78615941 None:intergenic 45.0%
AATCACGGTCTCCAAGAGAA+GGG - chr4_4:78616029-78616048 Msa0705820:CDS 45.0%
!! GGGCATTGCATCCATTTTCA+TGG - chr4_4:78616049-78616068 Msa0705820:CDS 45.0%
GATCGCCGACCCACTAATAT+TGG - chr4_4:78612541-78612560 Msa0705820:three_prime_UTR 50.0%
GATGATCTCTCTCCCACCAT+TGG - chr4_4:78612860-78612879 Msa0705820:exon 50.0%
TCTTACTACAGAGGGGCTCA+AGG - chr4_4:78613449-78613468 Msa0705820:intron 50.0%
AGGGGCTCAAGGAATCATCA+TGG - chr4_4:78613460-78613479 Msa0705820:intron 50.0%
GGGGCTCAAGGAATCATCAT+GGG - chr4_4:78613461-78613480 Msa0705820:intron 50.0%
TCAGCAAACATTCCCAGCAG+AGG + chr4_4:78613799-78613818 None:intergenic 50.0%
TCGCAGATCGCGGAAAATAG+CGG - chr4_4:78614709-78614728 Msa0705820:intron 50.0%
!! AGAGAGTACTGCAGAGAAGG+AGG - chr4_4:78614763-78614782 Msa0705820:intron 50.0%
!! GAGAGTACTGCAGAGAAGGA+GGG - chr4_4:78614764-78614783 Msa0705820:intron 50.0%
! GGTTACCAGCATGTTTCTGC+AGG - chr4_4:78614914-78614933 Msa0705820:intron 50.0%
AGCCTGTGCAATTTCGGGAA+AGG - chr4_4:78615743-78615762 Msa0705820:intron 50.0%
CTCCTTTCCCGAAATTGCAC+AGG + chr4_4:78615748-78615767 None:intergenic 50.0%
! GAACAATGGCTGATGGTGAC+TGG - chr4_4:78615843-78615862 Msa0705820:CDS 50.0%
! ACAATGGCTGATGGTGACTG+GGG - chr4_4:78615845-78615864 Msa0705820:CDS 50.0%
!! TACACCTAGCCTTTTAGCCG+AGG - chr4_4:78615902-78615921 Msa0705820:intron 50.0%
!! ACACCTAGCCTTTTAGCCGA+GGG - chr4_4:78615903-78615922 Msa0705820:intron 50.0%
!! CTTTTAGCCGAGGGCTCTAA+GGG - chr4_4:78615912-78615931 Msa0705820:intron 50.0%
ATAGCTTCAGCAACAACCGC+TGG + chr4_4:78615985-78616004 None:intergenic 50.0%
GAATCACGGTCTCCAAGAGA+AGG - chr4_4:78616028-78616047 Msa0705820:CDS 50.0%
TGGATGCAATGCCCTTCTCT+TGG + chr4_4:78616043-78616062 None:intergenic 50.0%
TTGTGGGAGGGGATGCTTGT+TGG - chr4_4:78612476-78612495 Msa0705820:three_prime_UTR 55.0%
TACTGCAGAGAAGGAGGGTG+AGG - chr4_4:78614769-78614788 Msa0705820:intron 55.0%
CCTTAGAGCCCTCGGCTAAA+AGG + chr4_4:78615914-78615933 None:intergenic 55.0%
! CCTTTTAGCCGAGGGCTCTA+AGG - chr4_4:78615911-78615930 Msa0705820:intron 55.0%
! CTGGTGGATGCATCAGACTG+AGG + chr4_4:78615966-78615985 None:intergenic 55.0%
CAGTCTGATGCATCCACCAG+CGG - chr4_4:78615966-78615985 Msa0705820:intron 55.0%
GAGCCCTCGGCTAAAAGGCT+AGG + chr4_4:78615909-78615928 None:intergenic 60.0%
GCTTCAGCAACAACCGCTGG+TGG + chr4_4:78615982-78616001 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 78612455 78616418 78612455 ID=Msa0705820;Name=Msa0705820
chr4_4 mRNA 78612455 78616418 78612455 ID=Msa0705820-mRNA-1;Parent=Msa0705820;Name=Msa0705820-mRNA-1;_AED=0.20;_eAED=0.20;_QI=183|0.62|0.66|1|0.5|0.55|9|420|286
chr4_4 exon 78612455 78612979 78612455 ID=Msa0705820-mRNA-1:exon:25160;Parent=Msa0705820-mRNA-1
chr4_4 exon 78613254 78613382 78613254 ID=Msa0705820-mRNA-1:exon:25159;Parent=Msa0705820-mRNA-1
chr4_4 exon 78614396 78614517 78614396 ID=Msa0705820-mRNA-1:exon:25158;Parent=Msa0705820-mRNA-1
chr4_4 exon 78614631 78614714 78614631 ID=Msa0705820-mRNA-1:exon:25157;Parent=Msa0705820-mRNA-1
chr4_4 exon 78614810 78614879 78614810 ID=Msa0705820-mRNA-1:exon:25156;Parent=Msa0705820-mRNA-1
chr4_4 exon 78615311 78615456 78615311 ID=Msa0705820-mRNA-1:exon:25155;Parent=Msa0705820-mRNA-1
chr4_4 exon 78615832 78615900 78615832 ID=Msa0705820-mRNA-1:exon:25154;Parent=Msa0705820-mRNA-1
chr4_4 exon 78615992 78616136 78615992 ID=Msa0705820-mRNA-1:exon:25153;Parent=Msa0705820-mRNA-1
chr4_4 exon 78616245 78616418 78616245 ID=Msa0705820-mRNA-1:exon:25152;Parent=Msa0705820-mRNA-1
chr4_4 five_prime_UTR 78616245 78616418 78616245 ID=Msa0705820-mRNA-1:five_prime_utr;Parent=Msa0705820-mRNA-1
chr4_4 five_prime_UTR 78616128 78616136 78616128 ID=Msa0705820-mRNA-1:five_prime_utr;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78615992 78616127 78615992 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78615832 78615900 78615832 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78615311 78615456 78615311 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78614810 78614879 78614810 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78614631 78614714 78614631 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78614396 78614517 78614396 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78613254 78613382 78613254 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 CDS 78612875 78612979 78612875 ID=Msa0705820-mRNA-1:cds;Parent=Msa0705820-mRNA-1
chr4_4 three_prime_UTR 78612455 78612874 78612455 ID=Msa0705820-mRNA-1:three_prime_utr;Parent=Msa0705820-mRNA-1
Gene Sequence

>Msa0705820

ATGGATTCGAGTTCAGGGCAGCAAGAATTCGATTATATGTTCAAATTGTTAATGATTGGGGATTCTGGTGTTGGCAAAAGTAGTCTTCTTCTCAGATTCACCTCAGATGATTTTGATGATCTCTCTCCCACCATTGGTGTTGATTTTAAGGTCAAATATGTCACGATAGAGGGTAAAAAGCTTAAGCTTGCCATTTGGGATACTGCTGGTCAGGAGAGATTTAGAACACTGACAAGTTCTTACTACAGAGGGGCTCAAGGAATCATCATGGGCAAGTTCCTATTCTCCCTTTTTTGCCTCTCAATTCTTCCAATAGTACATTTATTTACTGTGTTATGTGAAATGCCTCGATCTGTAGGATTCAATGGCCAGTATTCAAATCATGTATTTTTTGGCTTTAATGATTTAGATTCTCGTGAAAGATTCAATGGCCAAGTTGGACTTTCTTTGTTAGTGATGGGGTTCCCTGCCAAAATGACTGCAATATTTGTATTAGATGCAATCTTTTATGACGTAACACGGCGAGAAACATTTACAAATCTCTCTGAAATATGGGCAAAGGAAATAGACCTTTATTCAACAAATCAAGACTGCATGAAGATGCTTGTTGGAAACAAAGTAGATAAGGAGGGTGATAGAGTTGTGACAAAGAAAGAGGGAATAGACTTTGCTAGGGAATATGGTTGCCTATTTATTGAATGCAGTGCTAAAACTCGAGTAAATGTGCAGCAATGCTTTGAAGAGCTTGTTTTGAAGGTTCTTGATACACCTAGCCTTTTAGCCGAGGGCTCTAAGGGAGTAAAAAAGAACATTTTTAAGGACAAACCTCAGTCTGATGCATCCACCAGCGGTTGTTGCTGA
Protein sequence

>Msa0705820

MDSSSGQQEFDYMFKLLMIGDSGVGKSSLLLRFTSDDFDDLSPTIGVDFKVKYVTIEGKKLKLAIWDTAGQERFRTLTSSYYRGAQGIIMGKFLFSLFCLSILPIVHLFTVLCEMPRSVGFNGQYSNHVFFGFNDLDSRERFNGQVGLSLLVMGFPAKMTAIFVLDAIFYDVTRRETFTNLSEIWAKEIDLYSTNQDCMKMLVGNKVDKEGDRVVTKKEGIDFAREYGCLFIECSAKTRVNVQQCFEELVLKVLDTPSLLAEGSKGVKKNIFKDKPQSDASTSGCC*