AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0231380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0231380 MtrunA17_Chr2g0277971 97.959 147 3 0 1 147 1 147 3.84e-101 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0231380 AT1G60870.1 59.184 147 60 0 1 147 1 147 1.64e-33 115

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGAGAAAGCAAGAAATA+TGG 0.282094 2_2:-3607917 Msa0231380:CDS
GCTTGGAAAGGAATTTCTTC+TGG 0.317736 2_2:-3607808 Msa0231380:CDS
AGCAGCTGCCCATGCTTTGT+AGG 0.349595 2_2:+3608101 None:intergenic
AAGAAATATGGGAAACTTGA+TGG 0.389367 2_2:-3607905 Msa0231380:CDS
GTGCCATGGATGAATTTAGA+AGG 0.394252 2_2:-3607996 Msa0231380:CDS
GGAATATCTCGACTCGGCTA+TGG 0.422650 2_2:-3608022 Msa0231380:CDS
GAGGATTTCAAGAGATGAAG+TGG 0.462068 2_2:-3607956 Msa0231380:CDS
GCTGAGAAAGCAAGAAATAT+GGG 0.463308 2_2:-3607916 Msa0231380:CDS
ATAAGATCTTCGATCGTCGA+TGG 0.506443 2_2:+3608144 None:intergenic
GAAAGGAATTTCTTCTGGCA+AGG 0.513277 2_2:-3607803 Msa0231380:CDS
AGATAGAATCCTACAAAGCA+TGG 0.513381 2_2:-3608110 Msa0231380:CDS
AAAGGAAGGTGAACTGAGAA+AGG 0.516993 2_2:+3608199 None:intergenic
CTGCTTCTATGAAATCTGCT+TGG 0.529588 2_2:-3607825 Msa0231380:CDS
GGTTTGAAGAAGAACTTGAG+AGG 0.547329 2_2:-3607975 Msa0231380:CDS
AAACCTTCTAAATTCATCCA+TGG 0.552800 2_2:+3607993 None:intergenic
TAAAGATTGATCTGAAAGGA+AGG 0.570141 2_2:+3608185 None:intergenic
AAAGTATATATGTATCAAGA+TGG 0.576287 2_2:+3607775 None:intergenic
CTCGGCTATGGAAAGTGCCA+TGG 0.577831 2_2:-3608010 Msa0231380:CDS
TCTATGAAATCTGCTTGGAA+AGG 0.578134 2_2:-3607820 Msa0231380:CDS
CATGTAAAGATTGATCTGAA+AGG 0.594535 2_2:+3608181 None:intergenic
AGCCGAGTCGAGATATTCCT+CGG 0.604035 2_2:+3608026 None:intergenic
TGAACTTGAGCAAGAAAGAG+AGG 0.624850 2_2:-3608079 Msa0231380:CDS
AGCCGAGGAATATCTCGACT+CGG 0.631008 2_2:-3608028 Msa0231380:CDS
GATAGAATCCTACAAAGCAT+GGG 0.638623 2_2:-3608109 Msa0231380:CDS
AGATGCAATGCAAGAAGCCG+AGG 0.640190 2_2:-3608043 Msa0231380:CDS
ACTTGAGCAAGAAAGAGAGG+TGG 0.641638 2_2:-3608076 Msa0231380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAAAGATTGATCTGAAAGGA+AGG + chr2_2:3607817-3607836 None:intergenic 30.0%
CATGTAAAGATTGATCTGAA+AGG + chr2_2:3607821-3607840 None:intergenic 30.0%
AAACCTTCTAAATTCATCCA+TGG + chr2_2:3608009-3608028 None:intergenic 30.0%
AAGAAATATGGGAAACTTGA+TGG - chr2_2:3608094-3608113 Msa0231380:CDS 30.0%
AGATAGAATCCTACAAAGCA+TGG - chr2_2:3607889-3607908 Msa0231380:CDS 35.0%
GATAGAATCCTACAAAGCAT+GGG - chr2_2:3607890-3607909 Msa0231380:CDS 35.0%
AGCTGAGAAAGCAAGAAATA+TGG - chr2_2:3608082-3608101 Msa0231380:CDS 35.0%
GCTGAGAAAGCAAGAAATAT+GGG - chr2_2:3608083-3608102 Msa0231380:CDS 35.0%
TCTATGAAATCTGCTTGGAA+AGG - chr2_2:3608179-3608198 Msa0231380:CDS 35.0%
AAAGGAAGGTGAACTGAGAA+AGG + chr2_2:3607803-3607822 None:intergenic 40.0%
ATAAGATCTTCGATCGTCGA+TGG + chr2_2:3607858-3607877 None:intergenic 40.0%
TGAACTTGAGCAAGAAAGAG+AGG - chr2_2:3607920-3607939 Msa0231380:CDS 40.0%
GTGCCATGGATGAATTTAGA+AGG - chr2_2:3608003-3608022 Msa0231380:CDS 40.0%
GGTTTGAAGAAGAACTTGAG+AGG - chr2_2:3608024-3608043 Msa0231380:CDS 40.0%
GAGGATTTCAAGAGATGAAG+TGG - chr2_2:3608043-3608062 Msa0231380:CDS 40.0%
CTGCTTCTATGAAATCTGCT+TGG - chr2_2:3608174-3608193 Msa0231380:CDS 40.0%
GCTTGGAAAGGAATTTCTTC+TGG - chr2_2:3608191-3608210 Msa0231380:CDS 40.0%
GAAAGGAATTTCTTCTGGCA+AGG - chr2_2:3608196-3608215 Msa0231380:CDS 40.0%
ACTTGAGCAAGAAAGAGAGG+TGG - chr2_2:3607923-3607942 Msa0231380:CDS 45.0%
AGATGCAATGCAAGAAGCCG+AGG - chr2_2:3607956-3607975 Msa0231380:CDS 50.0%
AGCCGAGGAATATCTCGACT+CGG - chr2_2:3607971-3607990 Msa0231380:CDS 50.0%
AGCCGAGTCGAGATATTCCT+CGG + chr2_2:3607976-3607995 None:intergenic 50.0%
!! GGAATATCTCGACTCGGCTA+TGG - chr2_2:3607977-3607996 Msa0231380:CDS 50.0%
AGCAGCTGCCCATGCTTTGT+AGG + chr2_2:3607901-3607920 None:intergenic 55.0%
!! CTCGGCTATGGAAAGTGCCA+TGG - chr2_2:3607989-3608008 Msa0231380:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 3607789 3608232 3607789 ID=Msa0231380;Name=Msa0231380
chr2_2 mRNA 3607789 3608232 3607789 ID=Msa0231380-mRNA-1;Parent=Msa0231380;Name=Msa0231380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|147
chr2_2 exon 3607789 3608232 3607789 ID=Msa0231380-mRNA-1:exon:1465;Parent=Msa0231380-mRNA-1
chr2_2 CDS 3607789 3608232 3607789 ID=Msa0231380-mRNA-1:cds;Parent=Msa0231380-mRNA-1
Gene Sequence

>Msa0231380

ATGGAAGCTCTCCTTTCTCAGTTCACCTTCCTTTCAGATCAATCTTTACATGACAAGAGCTTCGATCCATCGACGATCGAAGATCTTATGAAACTATTTGAGATAGAATCCTACAAAGCATGGGCAGCTGCTGAACTTGAGCAAGAAAGAGAGGTGGAAGAAGCAGAAGATGCAATGCAAGAAGCCGAGGAATATCTCGACTCGGCTATGGAAAGTGCCATGGATGAATTTAGAAGGTTTGAAGAAGAACTTGAGAGGATTTCAAGAGATGAAGTGGAGAGTTTAGTTCAAACAGCTGAGAAAGCAAGAAATATGGGAAACTTGATGGAAAAAACAGCTTCTATTGCTTCTAAGAAATATATTGAAGCTGCACTTAATTCAGCTACTGCTTCTATGAAATCTGCTTGGAAAGGAATTTCTTCTGGCAAGGTTCATCCATCTTGA

Protein sequence

>Msa0231380

MEALLSQFTFLSDQSLHDKSFDPSTIEDLMKLFEIESYKAWAAAELEQEREVEEAEDAMQEAEEYLDSAMESAMDEFRRFEEELERISRDEVESLVQTAEKARNMGNLMEKTASIASKKYIEAALNSATASMKSAWKGISSGKVHPS*