AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006959

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006959.t1 MTR_4g084880 97.736 265 5 1 1 264 9 273 0.0 518
MS.gene006959.t1 MTR_8g087720 58.103 253 104 1 5 257 16 266 1.75e-108 315
MS.gene006959.t1 MTR_3g008920 63.203 231 82 1 17 247 16 243 8.53e-107 310
MS.gene006959.t1 MTR_5g079570 63.158 228 81 1 17 244 30 254 9.46e-107 310
MS.gene006959.t1 MTR_8g087710 56.202 258 108 2 10 264 19 274 3.11e-105 306
MS.gene006959.t1 MTR_2g017570 57.787 244 101 1 21 264 32 273 2.22e-103 301
MS.gene006959.t1 MTR_2g017590 57.500 240 100 1 23 262 32 269 8.23e-103 300
MS.gene006959.t1 MTR_2g017610 57.377 244 102 1 21 264 32 273 1.46e-102 300
MS.gene006959.t1 MTR_2g017660 53.333 240 110 1 5 244 17 254 9.93e-96 282
MS.gene006959.t1 MTR_2g017620 56.410 234 100 1 23 256 20 251 9.75e-93 274
MS.gene006959.t1 MTR_2g017620 56.410 234 100 1 23 256 34 265 1.47e-92 274
MS.gene006959.t1 MTR_7g010650 49.345 229 112 3 27 255 37 261 2.59e-76 233
MS.gene006959.t1 MTR_2g017620 57.609 184 78 0 73 256 1 184 2.07e-75 228
MS.gene006959.t1 MTR_8g087720 57.979 188 77 1 5 192 16 201 2.31e-73 223
MS.gene006959.t1 MTR_4g006730 46.768 263 126 4 1 260 54 305 2.07e-70 219
MS.gene006959.t1 MTR_2g104940 46.025 239 120 3 25 260 78 310 1.01e-68 215
MS.gene006959.t1 MTR_4g006630 48.416 221 111 2 40 260 1 218 1.47e-64 201
MS.gene006959.t1 MTR_4g006730 48.416 221 111 2 40 260 1 218 2.67e-64 200
MS.gene006959.t1 MTR_8g087720 56.548 168 71 1 5 172 16 181 1.12e-62 196
MS.gene006959.t1 MTR_1g097840 43.882 237 118 4 23 250 70 300 4.24e-61 195
MS.gene006959.t1 MTR_5g063930 41.739 230 124 4 26 251 36 259 7.68e-59 188
MS.gene006959.t1 MTR_4g006730 45.778 225 96 6 1 222 54 255 1.11e-46 157
MS.gene006959.t1 MTR_1g070385 37.209 215 132 2 29 243 64 275 1.72e-45 163
MS.gene006959.t1 MTR_7g010650 47.260 146 74 2 27 172 37 179 1.16e-41 142
MS.gene006959.t1 MTR_1g097840 43.353 173 89 3 23 192 70 236 6.80e-38 134
MS.gene006959.t1 MTR_5g012810 41.714 175 92 5 81 248 76 247 4.31e-27 105
MS.gene006959.t1 MTR_4g063090 39.080 174 96 3 81 248 78 247 6.36e-27 105
MS.gene006959.t1 MTR_2g101370 33.333 228 133 6 23 238 16 236 3.21e-26 103
MS.gene006959.t1 MTR_4g006650 46.939 147 60 4 28 171 5 136 4.15e-26 104
MS.gene006959.t1 MTR_1g077540 31.696 224 148 3 26 244 20 243 1.34e-25 102
MS.gene006959.t1 MTR_1g115405 34.454 238 139 5 26 252 20 251 3.43e-25 100
MS.gene006959.t1 MTR_8g013680 32.751 229 147 4 26 247 18 246 8.85e-25 99.4
MS.gene006959.t1 MTR_1g006490 33.182 220 130 6 26 236 18 229 6.86e-24 97.1
MS.gene006959.t1 MTR_6g069040 30.088 226 149 3 26 244 20 243 2.59e-23 95.5
MS.gene006959.t1 MTR_7g023350 31.984 247 152 5 15 252 12 251 2.86e-22 92.8
MS.gene006959.t1 MTR_1g006590 32.018 228 142 6 26 245 18 240 3.79e-21 89.7
MS.gene006959.t1 MTR_3g070210 27.876 226 143 5 30 238 58 280 4.20e-21 90.5
MS.gene006959.t1 MTR_2g094270 31.092 238 136 8 25 238 38 271 5.67e-21 90.1
MS.gene006959.t1 MTR_3g118010 29.515 227 138 6 27 236 37 258 2.45e-20 88.2
MS.gene006959.t1 MTR_5g082070 27.350 234 149 6 30 246 58 287 5.43e-20 87.4
MS.gene006959.t1 MTR_7g101190 28.346 254 152 8 27 255 39 287 1.72e-19 85.9
MS.gene006959.t1 MTR_5g010150 25.984 254 154 6 2 238 41 277 6.58e-19 84.3
MS.gene006959.t1 MTR_4g059390 31.771 192 111 5 63 238 82 269 7.08e-19 84.3
MS.gene006959.t1 MTR_7g103030 27.711 249 160 6 17 252 8 249 1.31e-18 82.8
MS.gene006959.t1 MTR_8g098375 25.100 251 160 5 2 238 41 277 6.95e-18 81.6
MS.gene006959.t1 MTR_4g415300 28.251 223 145 6 30 238 59 280 7.88e-17 78.6
MS.gene006959.t1 MTR_1g095070 29.508 183 109 4 72 238 25 203 6.23e-16 75.1
MS.gene006959.t1 MTR_3g070210 26.904 197 127 4 30 212 58 251 1.09e-15 75.5
MS.gene006959.t1 MTR_2g060360 29.064 203 112 4 75 264 70 253 2.85e-15 73.9
MS.gene006959.t1 MTR_2g094270 28.972 214 127 7 25 217 38 247 3.37e-15 73.9
MS.gene006959.t1 MTR_1g095070 28.947 152 91 3 72 210 25 172 5.00e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006959.t1 AT5G37820 64.368 261 90 2 1 260 16 274 1.14e-120 346
MS.gene006959.t1 AT5G37820 64.368 261 90 2 1 260 52 310 4.42e-120 346
MS.gene006959.t1 AT5G37810 65.000 260 88 2 1 259 16 273 2.13e-118 340
MS.gene006959.t1 AT4G18910 59.839 249 91 2 23 264 48 294 2.49e-105 307
MS.gene006959.t1 AT4G19030 55.061 247 102 2 23 262 51 295 7.28e-96 283
MS.gene006959.t1 AT1G31885 49.020 255 118 4 5 250 20 271 8.99e-81 246
MS.gene006959.t1 AT1G31885 51.818 220 95 3 39 250 1 217 4.47e-75 229
MS.gene006959.t1 AT2G34390 45.902 244 122 3 23 259 44 284 1.48e-70 219
MS.gene006959.t1 AT2G34390 45.607 239 120 3 28 259 7 242 1.65e-68 212
MS.gene006959.t1 AT4G18910 59.322 177 63 2 23 192 48 222 3.63e-67 208
MS.gene006959.t1 AT1G80760 44.488 254 129 4 1 252 59 302 2.29e-66 209
MS.gene006959.t1 AT4G19030 53.409 176 73 2 23 191 51 224 4.66e-61 192
MS.gene006959.t1 AT4G10380 43.983 241 119 5 19 250 71 304 3.96e-60 192
MS.gene006959.t1 AT4G19030 52.229 157 66 2 23 172 51 205 6.91e-52 169
MS.gene006959.t1 AT2G34390 46.893 177 84 3 23 192 44 217 4.28e-49 161
MS.gene006959.t1 AT3G06100 38.636 220 131 3 26 244 45 261 5.16e-48 160
MS.gene006959.t1 AT3G06100 38.889 216 128 3 30 244 1 213 1.18e-47 158
MS.gene006959.t1 AT2G34390 45.977 174 79 5 10 174 32 199 1.05e-43 148
MS.gene006959.t1 AT5G47450 34.335 233 144 4 11 236 3 233 1.87e-28 109
MS.gene006959.t1 AT3G26520 34.783 230 138 6 26 247 21 246 1.84e-27 106
MS.gene006959.t1 AT3G16240 33.333 222 131 5 26 236 18 233 3.23e-27 105
MS.gene006959.t1 AT4G01470 39.634 164 89 3 81 238 78 237 6.57e-26 102
MS.gene006959.t1 AT4G17340 35.465 172 106 2 81 247 76 247 2.32e-25 101
MS.gene006959.t1 AT1G17810 30.556 252 149 8 6 238 1 245 2.67e-25 101
MS.gene006959.t1 AT1G31885 46.602 103 50 3 5 105 20 119 6.81e-25 97.1
MS.gene006959.t1 AT3G06100 36.170 141 88 2 105 244 41 180 3.25e-24 96.7
MS.gene006959.t1 AT1G73190 30.233 258 154 8 6 244 1 251 3.56e-24 98.2
MS.gene006959.t1 AT2G36830 35.377 212 122 6 26 227 20 226 5.64e-24 97.4
MS.gene006959.t1 AT2G29870 39.655 116 70 0 144 259 20 135 6.06e-23 91.7
MS.gene006959.t1 AT2G16850 31.839 223 138 6 27 236 36 257 1.49e-21 91.7
MS.gene006959.t1 AT2G25810 31.646 237 156 4 11 244 3 236 2.04e-21 90.5
MS.gene006959.t1 AT2G37170 28.571 238 143 6 24 238 34 267 2.51e-20 88.2
MS.gene006959.t1 AT2G37170 28.571 238 143 6 24 238 77 310 3.34e-20 88.6
MS.gene006959.t1 AT2G37180 28.571 238 143 6 24 238 34 267 5.21e-20 87.4
MS.gene006959.t1 AT3G53420 28.085 235 142 5 27 238 39 269 5.42e-20 87.4
MS.gene006959.t1 AT3G53420 28.085 235 142 5 27 238 39 269 5.42e-20 87.4
MS.gene006959.t1 AT4G23400 25.197 254 169 5 2 238 28 277 2.06e-19 85.9
MS.gene006959.t1 AT1G01620 27.876 226 142 5 30 238 55 276 2.64e-19 85.5
MS.gene006959.t1 AT4G35100 30.493 223 141 6 27 236 38 259 3.74e-19 85.1
MS.gene006959.t1 AT4G35100 30.493 223 141 6 27 236 38 259 3.74e-19 85.1
MS.gene006959.t1 AT5G60660 27.848 237 144 5 25 238 37 269 5.54e-19 84.7
MS.gene006959.t1 AT4G00430 29.185 233 151 6 30 249 56 287 9.70e-19 84.0
MS.gene006959.t1 AT3G61430 29.185 233 151 6 30 249 55 286 1.60e-18 83.2
MS.gene006959.t1 AT3G61430 29.185 233 151 6 30 249 55 286 1.60e-18 83.2
MS.gene006959.t1 AT2G39010 28.085 235 142 5 27 238 38 268 1.82e-18 83.2
MS.gene006959.t1 AT2G45960 27.897 233 154 6 30 249 55 286 1.39e-17 80.9
MS.gene006959.t1 AT1G01620 26.699 206 131 4 49 238 3 204 1.58e-17 79.3
MS.gene006959.t1 AT3G54820 26.383 235 146 5 27 238 38 268 1.21e-16 78.2
MS.gene006959.t1 AT3G47440 29.630 216 138 6 30 236 26 236 6.92e-15 72.8
MS.gene006959.t1 AT2G45960 25.490 204 142 4 30 223 55 258 1.73e-11 63.5
MS.gene006959.t1 AT2G45960 27.461 193 130 4 30 212 55 247 1.80e-11 63.2

Find 55 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCACATCTACAAAGGATTT+TGG 0.135458 4.4:-26925455 MS.gene006959:CDS
ATGAACCCAGCCAGAACTTT+TGG 0.245283 4.4:-26925492 MS.gene006959:CDS
CCCTTTGTTGGTGCTATATT+AGG 0.248716 4.4:-26925417 MS.gene006959:CDS
ATGATCTTGGTTTATTCTCT+TGG 0.315998 4.4:-26926427 MS.gene006959:CDS
TATCAATCAGATTGGAGAAT+TGG 0.321256 4.4:-26925693 MS.gene006959:intron
ACATACTTCTTAATATTTGC+AGG 0.325876 4.4:-26926538 MS.gene006959:CDS
TATTCTCTTGGTCACATCTC+TGG 0.351110 4.4:-26926415 MS.gene006959:CDS
TACAGCAGGCCCAAAAGTTC+TGG 0.354194 4.4:+26925482 None:intergenic
ACCTGTGCAGCAAGATATAA+TGG 0.385059 4.4:+26926151 None:intergenic
CTGTGACTGCTGGATTGAAA+TGG 0.387793 4.4:+26926389 None:intergenic
AAAGGAACAGTAACATTTCC+TGG 0.388672 4.4:-26926478 MS.gene006959:CDS
TATATAGTTGGACCCTTTGT+TGG 0.388837 4.4:-26925429 MS.gene006959:CDS
GTAGTAGACGTCTTCATTGC+TGG 0.394702 4.4:-26925644 MS.gene006959:CDS
TCGTCATTTCCCGCTTAAAC+AGG 0.397872 4.4:-26926350 MS.gene006959:intron
GGGTCTATCAGTTATGATCT+TGG 0.398096 4.4:-26926440 MS.gene006959:CDS
CCCCATGTTACACAAATTCC+AGG 0.411162 4.4:+26926460 None:intergenic
AATGGAAGAAGGTGATCTTA+AGG 0.450995 4.4:-26926792 MS.gene006959:CDS
TTCCTGGAATTTGTGTAACA+TGG 0.464453 4.4:-26926462 MS.gene006959:CDS
TGTATGATTTGAACTACCTC+AGG 0.467934 4.4:+26926734 None:intergenic
CTAGGATCTGTCCTTGCTAG+TGG 0.477384 4.4:-26926127 MS.gene006959:CDS
AATCAGATTGGAGAATTGGC+AGG 0.484184 4.4:-26925689 MS.gene006959:intron
ATTGCATGCAGCCCTGTGTC+AGG 0.484373 4.4:-26925522 MS.gene006959:intron
TCCTGGAATTTGTGTAACAT+GGG 0.487395 4.4:-26926461 MS.gene006959:CDS
GCCATTATATCTTGCTGCAC+AGG 0.493573 4.4:-26926152 MS.gene006959:CDS
TATCTTGCTGCACAGGTGCT+AGG 0.498223 4.4:-26926145 MS.gene006959:CDS
GCAGGCCCAAAAGTTCTGGC+TGG 0.505285 4.4:+26925486 None:intergenic
TGTGACTGCTGGATTGAAAT+GGG 0.509378 4.4:+26926390 None:intergenic
GGTAGTTCAAATCATACAAA+AGG 0.511595 4.4:-26926729 MS.gene006959:intron
TTCATTGATGCACCTGACAC+AGG 0.513121 4.4:+26925510 None:intergenic
CGTAGTGGTTCTGAATAAAG+TGG 0.538283 4.4:-26926509 MS.gene006959:CDS
TGTTGTATCTGCAGTTTCCA+CGG 0.547342 4.4:-26925990 MS.gene006959:CDS
AATAAAGTGGAAGAAACTAA+AGG 0.547480 4.4:-26926496 MS.gene006959:CDS
TAGTAGACGTCTTCATTGCT+GGG 0.549173 4.4:-26925643 MS.gene006959:intron
GAGATCACGAAAATGGAAGA+AGG 0.552927 4.4:-26926803 None:intergenic
CATACTTCTTAATATTTGCA+GGG 0.553842 4.4:-26926537 MS.gene006959:CDS
CAGGTAATAGCAGAGGTGAT+AGG 0.557378 4.4:-26926562 MS.gene006959:CDS
GCGAAGCTAACTGTGACTGC+TGG 0.557473 4.4:+26926379 None:intergenic
ATATGCATGCCTGTTTAAGC+GGG 0.559351 4.4:+26926341 None:intergenic
TAGATGTGCATCACTACAGC+AGG 0.560341 4.4:+26925468 None:intergenic
TATATGCATGCCTGTTTAAG+CGG 0.569385 4.4:+26926340 None:intergenic
CCTAATATAGCACCAACAAA+GGG 0.576824 4.4:+26925417 None:intergenic
CAGGCCCAAAAGTTCTGGCT+GGG 0.576999 4.4:+26925487 None:intergenic
ACCTAATATAGCACCAACAA+AGG 0.581057 4.4:+26925416 None:intergenic
GGCACAATGTTCCACTAGCA+AGG 0.584967 4.4:+26926116 None:intergenic
ATCAGATTGGAGAATTGGCA+GGG 0.586964 4.4:-26925688 MS.gene006959:intron
GAATTGGCAGGGATTGCTGT+TGG 0.598721 4.4:-26925677 MS.gene006959:CDS
ATTTGCAGGGTGTTGCGTAG+TGG 0.602223 4.4:-26926524 MS.gene006959:CDS
CCTGGAATTTGTGTAACATG+GGG 0.603385 4.4:-26926460 MS.gene006959:CDS
GATTGAATATTAGAACCTGC+TGG 0.604048 4.4:+26926052 None:intergenic
TCATTGATGCACCTGACACA+GGG 0.615117 4.4:+26925511 None:intergenic
CTTCGGCTTAATTCTCTGAG+TGG 0.624993 4.4:+26925357 None:intergenic
ACTAACCGCTCTATTGTCCG+TGG 0.634378 4.4:+26925973 None:intergenic
GTAGTGATGCACATCTACAA+AGG 0.641675 4.4:-26925462 MS.gene006959:CDS
TATGCAACAGGTAATAGCAG+AGG 0.665558 4.4:-26926569 MS.gene006959:intron
AGTTTCCACGGACAATAGAG+CGG 0.688862 4.4:-26925978 MS.gene006959:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATTAATGTGATTGTA+TGG + chr4.4:26925430-26925449 None:intergenic 15.0%
!! TACTATATATATAGTTTCAA+TGG - chr4.4:26926500-26926519 MS.gene006959:CDS 15.0%
!!! AAATTTTGTTTTGAAAATAG+TGG + chr4.4:26926316-26926335 None:intergenic 15.0%
!!! AGGAATTTGAATTAATTTTA+TGG + chr4.4:26926387-26926406 None:intergenic 15.0%
!!! CATTTTGGTATATAAAATTA+GGG - chr4.4:26926192-26926211 MS.gene006959:intron 15.0%
!!! TTGAATTAATTTTATGGAAA+AGG + chr4.4:26926381-26926400 None:intergenic 15.0%
!! CAAAACAAAATTTCTATACT+AGG - chr4.4:26926323-26926342 MS.gene006959:intron 20.0%
!!! GCATTTTGGTATATAAAATT+AGG - chr4.4:26926191-26926210 MS.gene006959:intron 20.0%
!!! GTAGATGAAAAATCTTTTTT+TGG - chr4.4:26926023-26926042 MS.gene006959:CDS 20.0%
! AAAAATGAAATGTATGCAAC+AGG - chr4.4:26925524-26925543 MS.gene006959:intron 25.0%
! AAATTGCAGCAGTATATATA+TGG + chr4.4:26926547-26926566 None:intergenic 25.0%
! AATAAAGTGGAAGAAACTAA+AGG - chr4.4:26925609-26925628 MS.gene006959:intron 25.0%
! ACATACTTCTTAATATTTGC+AGG - chr4.4:26925567-26925586 MS.gene006959:intron 25.0%
! CAGTAAATACTTACTATTAG+TGG + chr4.4:26926257-26926276 None:intergenic 25.0%
! CATACTTCTTAATATTTGCA+GGG - chr4.4:26925568-26925587 MS.gene006959:intron 25.0%
! CCAAAATGCAAGAAAATATA+AGG + chr4.4:26926180-26926199 None:intergenic 25.0%
! TTTCATCTACATCAAAAAGA+AGG + chr4.4:26926013-26926032 None:intergenic 25.0%
!! CCTTATATTTTCTTGCATTT+TGG - chr4.4:26926177-26926196 MS.gene006959:intron 25.0%
!!! CATAAATTGTTGATTTTTGC+AGG - chr4.4:26925929-26925948 MS.gene006959:intron 25.0%
AATGTGATTGTATGGTACAA+TGG + chr4.4:26925422-26925441 None:intergenic 30.0%
ATATGTGTACAATGGTACAA+TGG + chr4.4:26925475-26925494 None:intergenic 30.0%
ATGATCTTGGTTTATTCTCT+TGG - chr4.4:26925678-26925697 MS.gene006959:CDS 30.0%
CACAAACAAAAAGTGAATCA+GGG - chr4.4:26925336-26925355 MS.gene006959:CDS 30.0%
CCAATCTGATTGATAAAAAG+AGG + chr4.4:26926407-26926426 None:intergenic 30.0%
GGTAGTTCAAATCATACAAA+AGG - chr4.4:26925376-26925395 MS.gene006959:CDS 30.0%
TATCAATCAGATTGGAGAAT+TGG - chr4.4:26926412-26926431 MS.gene006959:CDS 30.0%
TGACAAAGATATGTGTACAA+TGG + chr4.4:26925483-26925502 None:intergenic 30.0%
TGCATGCAATTATCAAACTT+AGG + chr4.4:26926573-26926592 None:intergenic 30.0%
! CCTCTTTTTATCAATCAGAT+TGG - chr4.4:26926404-26926423 MS.gene006959:CDS 30.0%
!!! GGATTTTGGGTTTATATAGT+TGG - chr4.4:26926664-26926683 MS.gene006959:intron 30.0%
AAAAGGCTAACATGTGTCTT+AGG + chr4.4:26926364-26926383 None:intergenic 35.0%
AAAGGAACAGTAACATTTCC+TGG - chr4.4:26925627-26925646 MS.gene006959:intron 35.0%
AAAGGCTAACATGTGTCTTA+GGG + chr4.4:26926363-26926382 None:intergenic 35.0%
ACCTAATATAGCACCAACAA+AGG + chr4.4:26926692-26926711 None:intergenic 35.0%
CCTAATATAGCACCAACAAA+GGG + chr4.4:26926691-26926710 None:intergenic 35.0%
CTTAACGACTGAATTAGAGA+TGG - chr4.4:26925880-26925899 MS.gene006959:intron 35.0%
GATTGAATATTAGAACCTGC+TGG + chr4.4:26926056-26926075 None:intergenic 35.0%
GCACAAACAAAAAGTGAATC+AGG - chr4.4:26925335-26925354 MS.gene006959:CDS 35.0%
TATATAGTTGGACCCTTTGT+TGG - chr4.4:26926676-26926695 MS.gene006959:intron 35.0%
TATATGCATGCCTGTTTAAG+CGG + chr4.4:26925768-26925787 None:intergenic 35.0%
TCCTGGAATTTGTGTAACAT+GGG - chr4.4:26925644-26925663 MS.gene006959:CDS 35.0%
TGTATGATTTGAACTACCTC+AGG + chr4.4:26925374-26925393 None:intergenic 35.0%
TTCCTGGAATTTGTGTAACA+TGG - chr4.4:26925643-26925662 MS.gene006959:intron 35.0%
! AAAAGTGAATCAGGGTTTTG+TGG - chr4.4:26925344-26925363 MS.gene006959:CDS 35.0%
! AAGTGTTTGAGACGACAAAA+TGG + chr4.4:26925863-26925882 None:intergenic 35.0%
! AGTGTTTGAGACGACAAAAT+GGG + chr4.4:26925862-26925881 None:intergenic 35.0%
! GCACATCTACAAAGGATTTT+GGG - chr4.4:26926651-26926670 MS.gene006959:intron 35.0%
! TGCACATCTACAAAGGATTT+TGG - chr4.4:26926650-26926669 MS.gene006959:intron 35.0%
AATCAGATTGGAGAATTGGC+AGG - chr4.4:26926416-26926435 MS.gene006959:CDS 40.0%
ACCTGTGCAGCAAGATATAA+TGG + chr4.4:26925957-26925976 None:intergenic 40.0%
ATATGCATGCCTGTTTAAGC+GGG + chr4.4:26925767-26925786 None:intergenic 40.0%
ATCAGATTGGAGAATTGGCA+GGG - chr4.4:26926417-26926436 MS.gene006959:CDS 40.0%
CATGCTAATATGTGCCCTAA+GGG - chr4.4:26926216-26926235 MS.gene006959:intron 40.0%
CCTGGAATTTGTGTAACATG+GGG - chr4.4:26925645-26925664 MS.gene006959:CDS 40.0%
GGGTCTATCAGTTATGATCT+TGG - chr4.4:26925665-26925684 MS.gene006959:CDS 40.0%
GTAGTGATGCACATCTACAA+AGG - chr4.4:26926643-26926662 MS.gene006959:intron 40.0%
TAGTAGACGTCTTCATTGCT+GGG - chr4.4:26926462-26926481 MS.gene006959:CDS 40.0%
TATGCAACAGGTAATAGCAG+AGG - chr4.4:26925536-26925555 MS.gene006959:intron 40.0%
TATTCTCTTGGTCACATCTC+TGG - chr4.4:26925690-26925709 MS.gene006959:intron 40.0%
TCATGCTAATATGTGCCCTA+AGG - chr4.4:26926215-26926234 MS.gene006959:intron 40.0%
TGTGACTGCTGGATTGAAAT+GGG + chr4.4:26925718-26925737 None:intergenic 40.0%
TGTTGTATCTGCAGTTTCCA+CGG - chr4.4:26926115-26926134 MS.gene006959:CDS 40.0%
!! CCCTTTGTTGGTGCTATATT+AGG - chr4.4:26926688-26926707 MS.gene006959:intron 40.0%
!! CGTAGTGGTTCTGAATAAAG+TGG - chr4.4:26925596-26925615 MS.gene006959:intron 40.0%
!! TTTTGAGGAAGCTTGAGCTT+CGG + chr4.4:26926768-26926787 None:intergenic 40.0%
!!! GTGTCTCTAGAGCAAGTTTT+CGG - chr4.4:26926788-26926807 MS.gene006959:CDS 40.0%
!!! TTGCTCTAGAGACACTTTTG+AGG + chr4.4:26926783-26926802 None:intergenic 40.0%
!!! TTTTTTGGAACAGTACCAGC+AGG - chr4.4:26926038-26926057 MS.gene006959:CDS 40.0%
AGTTTCCACGGACAATAGAG+CGG - chr4.4:26926127-26926146 MS.gene006959:CDS 45.0%
CAGGTAATAGCAGAGGTGAT+AGG - chr4.4:26925543-26925562 MS.gene006959:intron 45.0%
CCCCATGTTACACAAATTCC+AGG + chr4.4:26925648-26925667 None:intergenic 45.0%
CTGTGACTGCTGGATTGAAA+TGG + chr4.4:26925719-26925738 None:intergenic 45.0%
CTTCGGCTTAATTCTCTGAG+TGG + chr4.4:26926751-26926770 None:intergenic 45.0%
GCCATTATATCTTGCTGCAC+AGG - chr4.4:26925953-26925972 MS.gene006959:intron 45.0%
GTAGTAGACGTCTTCATTGC+TGG - chr4.4:26926461-26926480 MS.gene006959:CDS 45.0%
TAGATGTGCATCACTACAGC+AGG + chr4.4:26926640-26926659 None:intergenic 45.0%
TCATTGATGCACCTGACACA+GGG + chr4.4:26926597-26926616 None:intergenic 45.0%
TCGTCATTTCCCGCTTAAAC+AGG - chr4.4:26925755-26925774 MS.gene006959:intron 45.0%
TTCATTGATGCACCTGACAC+AGG + chr4.4:26926598-26926617 None:intergenic 45.0%
! AATCCCTAACTTGTGCCCTT+AGG + chr4.4:26926234-26926253 None:intergenic 45.0%
! ATCCCTAACTTGTGCCCTTA+GGG + chr4.4:26926233-26926252 None:intergenic 45.0%
! ATGAACCCAGCCAGAACTTT+TGG - chr4.4:26926613-26926632 MS.gene006959:intron 45.0%
! TGAACCCAGCCAGAACTTTT+GGG - chr4.4:26926614-26926633 MS.gene006959:intron 45.0%
ACTAACCGCTCTATTGTCCG+TGG + chr4.4:26926135-26926154 None:intergenic 50.0%
CTAGGATCTGTCCTTGCTAG+TGG - chr4.4:26925978-26925997 MS.gene006959:intron 50.0%
GAATTGGCAGGGATTGCTGT+TGG - chr4.4:26926428-26926447 MS.gene006959:CDS 50.0%
GGCACAATGTTCCACTAGCA+AGG + chr4.4:26925992-26926011 None:intergenic 50.0%
TACAGCAGGCCCAAAAGTTC+TGG + chr4.4:26926626-26926645 None:intergenic 50.0%
TATCTTGCTGCACAGGTGCT+AGG - chr4.4:26925960-26925979 MS.gene006959:intron 50.0%
! TGCCCTAAGGGCACAAGTTA+GGG - chr4.4:26926228-26926247 MS.gene006959:intron 50.0%
!! AGGGTTTTGTGGTTCTCCTG+AGG - chr4.4:26925355-26925374 MS.gene006959:CDS 50.0%
!! ATTTGCAGGGTGTTGCGTAG+TGG - chr4.4:26925581-26925600 MS.gene006959:intron 50.0%
ATTGCATGCAGCCCTGTGTC+AGG - chr4.4:26926583-26926602 MS.gene006959:intron 55.0%
GCGAAGCTAACTGTGACTGC+TGG + chr4.4:26925729-26925748 None:intergenic 55.0%
! CAGGCCCAAAAGTTCTGGCT+GGG + chr4.4:26926621-26926640 None:intergenic 55.0%
! GTGCCCTAAGGGCACAAGTT+AGG - chr4.4:26926227-26926246 MS.gene006959:intron 55.0%
! GCAGGCCCAAAAGTTCTGGC+TGG + chr4.4:26926622-26926641 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 26925314 26926813 26925314 ID=MS.gene006959
chr4.4 mRNA 26925314 26926813 26925314 ID=MS.gene006959.t1;Parent=MS.gene006959
chr4.4 exon 26926730 26926813 26926730 ID=MS.gene006959.t1.exon1;Parent=MS.gene006959.t1
chr4.4 CDS 26926730 26926813 26926730 ID=cds.MS.gene006959.t1;Parent=MS.gene006959.t1
chr4.4 exon 26926351 26926581 26926351 ID=MS.gene006959.t1.exon2;Parent=MS.gene006959.t1
chr4.4 CDS 26926351 26926581 26926351 ID=cds.MS.gene006959.t1;Parent=MS.gene006959.t1
chr4.4 exon 26925979 26926176 26925979 ID=MS.gene006959.t1.exon3;Parent=MS.gene006959.t1
chr4.4 CDS 26925979 26926176 26925979 ID=cds.MS.gene006959.t1;Parent=MS.gene006959.t1
chr4.4 exon 26925644 26925705 26925644 ID=MS.gene006959.t1.exon4;Parent=MS.gene006959.t1
chr4.4 CDS 26925644 26925705 26925644 ID=cds.MS.gene006959.t1;Parent=MS.gene006959.t1
chr4.4 exon 26925314 26925533 26925314 ID=MS.gene006959.t1.exon5;Parent=MS.gene006959.t1
chr4.4 CDS 26925314 26925533 26925314 ID=cds.MS.gene006959.t1;Parent=MS.gene006959.t1
Gene Sequence

>MS.gene006959

ATGGAAGAAGGTGATCTTAAGGCACAAACAAAAAGTGAATCAGGGTTTTGTGGTTCTCCTGAGGTAGTTCAAATCATACAAAAGGTACACATATTAATAATTCTACCATTGTACCATACAATCACATTAATAATATATAGTTTTCATTTATATAGTTACCATTGTACCATTGTACACATATCTTTGTCACTACTAATGAATGTTTTATTTAAAAATGAAATGTATGCAACAGGTAATAGCAGAGGTGATAGGAACATACTTCTTAATATTTGCAGGGTGTTGCGTAGTGGTTCTGAATAAAGTGGAAGAAACTAAAGGAACAGTAACATTTCCTGGAATTTGTGTAACATGGGGTCTATCAGTTATGATCTTGGTTTATTCTCTTGGTCACATCTCTGGTGCCCATTTCAATCCAGCAGTCACAGTTAGCTTCGCAATCTATCGTCATTTCCCGCTTAAACAGGCATGCATATACTTATGACAACTTGATCTACTCAGCTCACATTGTTATAAAAAGTTGAATTAACGAAAAAAATAATTACTCTCCCATTTTGTCGTCTCAAACACTTAACGACTGAATTAGAGATGGATTTCTTGTTTTTCGAACATATGTAACATAAATTGTTGATTTTTGCAGGTGCCATTATATCTTGCTGCACAGGTGCTAGGATCTGTCCTTGCTAGTGGAACATTGTGCCTTCTTTTTGATGTAGATGAAAAATCTTTTTTTGGAACAGTACCAGCAGGTTCTAATATTCAATCTCTTGTCTTTGAGATACTCACATCATTTCTCTTGATGTTTGTTGTATCTGCAGTTTCCACGGACAATAGAGCGGTTAGTATACTCAGTTTAGTTTAATTTACCTTATATTTTCTTGCATTTTGGTATATAAAATTAGGGTCATGCTAATATGTGCCCTAAGGGCACAAGTTAGGGATTCCACTAATAGTAAGTATTTACTGAGAAACATTAATTTTCAATTTTTTAATAATAAATTGCCACTATTTTCAAAACAAAATTTCTATACTAGGTTCATTCACATGTGCCCTAAGACACATGTTAGCCTTTTCCATAAAATTAATTCAAATTCCTCTTTTTATCAATCAGATTGGAGAATTGGCAGGGATTGCTGTTGGCATGACAATCGTAGTAGACGTCTTCATTGCTGGGTAATTTCAACACTATTACTATATATATAGTTTCAATGGTTTTACTTATCATAATGAAGTCCATATATATACTGCTGCAATTTAATCCTAAGTTTGATAATTGCATGCAGCCCTGTGTCAGGTGCATCAATGAACCCAGCCAGAACTTTTGGGCCTGCTGTAGTGATGCACATCTACAAAGGATTTTGGGTTTATATAGTTGGACCCTTTGTTGGTGCTATATTAGGTGCATCAGCTTACAACTTGATTAGATTTACTGATAAACCACTCAGAGAATTAAGCCGAAGCTCAAGCTTCCTCAAAAGTGTCTCTAGAGCAAGTTTTCGGTAG
Protein sequence

>MS.gene006959.t1

MEEGDLKAQTKSESGFCGSPEVVQIIQKVIAEVIGTYFLIFAGCCVVVLNKVEETKGTVTFPGICVTWGLSVMILVYSLGHISGAHFNPAVTVSFAIYRHFPLKQVPLYLAAQVLGSVLASGTLCLLFDVDEKSFFGTVPAGSNIQSLVFEILTSFLLMFVVSAVSTDNRAIGELAGIAVGMTIVVDVFIAGPVSGASMNPARTFGPAVVMHIYKGFWVYIVGPFVGAILGASAYNLIRFTDKPLRELSRSSSFLKSVSRASFR