AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006960.t1 MTR_4g084880 98.312 237 3 1 1 236 37 273 2.12e-167 462
MS.gene006960.t1 MTR_8g087720 62.445 229 84 1 1 229 40 266 3.93e-106 307
MS.gene006960.t1 MTR_8g087710 58.051 236 97 1 1 236 41 274 1.42e-102 298
MS.gene006960.t1 MTR_5g079570 64.352 216 74 1 1 216 42 254 3.62e-102 297
MS.gene006960.t1 MTR_3g008920 63.927 219 76 1 1 219 28 243 8.94e-102 296
MS.gene006960.t1 MTR_2g017570 58.475 236 96 1 1 236 40 273 8.56e-101 294
MS.gene006960.t1 MTR_2g017590 57.692 234 97 1 1 234 38 269 2.90e-100 292
MS.gene006960.t1 MTR_2g017610 58.051 236 97 1 1 236 40 273 6.05e-100 292
MS.gene006960.t1 MTR_2g017660 57.407 216 90 1 1 216 41 254 1.49e-92 273
MS.gene006960.t1 MTR_2g017620 56.579 228 97 1 1 228 26 251 1.03e-90 268
MS.gene006960.t1 MTR_2g017620 56.579 228 97 1 1 228 40 265 1.46e-90 268
MS.gene006960.t1 MTR_7g010650 49.339 227 111 3 1 227 39 261 1.33e-75 230
MS.gene006960.t1 MTR_2g017620 57.609 184 78 0 45 228 1 184 1.34e-75 227
MS.gene006960.t1 MTR_8g087720 64.024 164 57 1 1 164 40 201 3.04e-71 216
MS.gene006960.t1 MTR_4g006730 48.927 233 110 3 3 232 79 305 4.84e-68 212
MS.gene006960.t1 MTR_2g104940 46.781 233 115 3 3 232 84 310 5.37e-68 212
MS.gene006960.t1 MTR_4g006630 48.416 221 111 2 12 232 1 218 6.27e-65 201
MS.gene006960.t1 MTR_4g006730 48.416 221 111 2 12 232 1 218 1.23e-64 200
MS.gene006960.t1 MTR_8g087720 63.194 144 51 1 1 144 40 181 1.20e-60 189
MS.gene006960.t1 MTR_1g097840 44.105 229 113 4 3 222 78 300 1.31e-58 187
MS.gene006960.t1 MTR_5g063930 41.150 226 123 4 2 223 40 259 8.50e-57 182
MS.gene006960.t1 MTR_1g070385 37.209 215 132 2 1 215 64 275 3.29e-45 161
MS.gene006960.t1 MTR_4g006730 48.205 195 80 5 3 194 79 255 3.80e-44 149
MS.gene006960.t1 MTR_7g010650 47.222 144 73 2 1 144 39 179 7.97e-41 139
MS.gene006960.t1 MTR_1g097840 43.636 165 84 3 3 164 78 236 8.38e-36 128
MS.gene006960.t1 MTR_5g012810 41.954 174 91 5 53 219 76 246 3.66e-27 104
MS.gene006960.t1 MTR_4g063090 39.080 174 96 3 53 220 78 247 5.30e-27 104
MS.gene006960.t1 MTR_4g006650 46.528 144 59 4 3 143 8 136 1.35e-25 102
MS.gene006960.t1 MTR_2g101370 33.484 221 128 6 2 210 23 236 1.66e-25 100
MS.gene006960.t1 MTR_1g115405 38.202 178 100 3 53 224 78 251 7.41e-25 99.0
MS.gene006960.t1 MTR_1g077540 31.818 220 145 3 2 216 24 243 9.55e-25 99.0
MS.gene006960.t1 MTR_8g013680 32.889 225 144 4 2 219 22 246 2.30e-24 97.4
MS.gene006960.t1 MTR_6g069040 33.735 166 108 1 53 216 78 243 4.88e-23 94.4
MS.gene006960.t1 MTR_1g006490 32.719 217 129 6 1 208 21 229 2.43e-22 92.0
MS.gene006960.t1 MTR_7g023350 37.079 178 102 3 53 224 78 251 9.36e-22 90.9
MS.gene006960.t1 MTR_3g070210 29.545 220 135 5 2 204 58 274 6.00e-21 89.4
MS.gene006960.t1 MTR_2g094270 32.051 234 131 9 1 210 42 271 9.68e-21 88.6
MS.gene006960.t1 MTR_3g118010 29.778 225 136 6 1 208 39 258 5.25e-20 86.7
MS.gene006960.t1 MTR_5g082070 29.091 220 136 5 2 204 58 274 8.56e-20 86.3
MS.gene006960.t1 MTR_1g006590 31.556 225 141 6 1 217 21 240 9.13e-20 85.1
MS.gene006960.t1 MTR_7g101190 30.332 211 124 6 35 227 82 287 1.88e-19 85.1
MS.gene006960.t1 MTR_4g059390 31.771 192 111 5 35 210 82 269 9.28e-19 83.2
MS.gene006960.t1 MTR_5g010150 26.991 226 145 5 2 210 55 277 1.19e-18 82.8
MS.gene006960.t1 MTR_7g103030 28.755 233 149 5 2 224 24 249 1.49e-18 82.0
MS.gene006960.t1 MTR_8g098375 26.009 223 151 4 2 210 55 277 1.05e-17 80.5
MS.gene006960.t1 MTR_4g415300 28.251 223 145 6 2 210 59 280 1.23e-16 77.4
MS.gene006960.t1 MTR_1g095070 29.508 183 109 4 44 210 25 203 4.67e-16 74.7
MS.gene006960.t1 MTR_2g060360 29.064 203 112 4 47 236 70 253 1.39e-15 73.9
MS.gene006960.t1 MTR_3g070210 26.904 197 127 4 2 184 58 251 1.83e-15 73.9
MS.gene006960.t1 MTR_2g094270 30.476 210 121 7 1 189 42 247 5.62e-15 72.4
MS.gene006960.t1 MTR_1g095070 28.662 157 95 3 44 187 25 177 4.16e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006960.t1 AT5G37820 67.094 234 75 1 1 234 45 276 1.17e-112 324
MS.gene006960.t1 AT5G37810 68.398 231 71 1 1 231 45 273 3.64e-112 323
MS.gene006960.t1 AT5G37820 67.094 234 75 1 1 234 81 312 6.22e-112 324
MS.gene006960.t1 AT4G18910 60.082 243 88 2 1 236 54 294 4.53e-103 300
MS.gene006960.t1 AT4G19030 55.187 241 99 2 1 234 57 295 9.62e-94 277
MS.gene006960.t1 AT1G31885 51.304 230 101 3 1 222 45 271 4.76e-78 238
MS.gene006960.t1 AT1G31885 51.818 220 95 3 11 222 1 217 4.81e-75 228
MS.gene006960.t1 AT2G34390 45.798 238 119 3 1 231 8 242 1.22e-68 211
MS.gene006960.t1 AT2G34390 45.798 238 119 3 1 231 50 284 3.52e-68 211
MS.gene006960.t1 AT4G18910 59.649 171 60 2 1 164 54 222 3.40e-65 202
MS.gene006960.t1 AT1G80760 46.875 224 112 3 3 224 84 302 1.90e-64 202
MS.gene006960.t1 AT4G19030 53.529 170 70 2 1 163 57 224 5.20e-59 186
MS.gene006960.t1 AT4G10380 44.541 229 112 4 3 222 82 304 2.01e-58 187
MS.gene006960.t1 AT4G19030 52.318 151 63 2 1 144 57 205 7.17e-50 162
MS.gene006960.t1 AT3G06100 38.889 216 128 3 2 216 1 213 5.08e-48 158
MS.gene006960.t1 AT3G06100 39.171 217 128 3 1 216 48 261 6.38e-48 159
MS.gene006960.t1 AT2G34390 46.784 171 81 3 1 164 50 217 1.25e-46 154
MS.gene006960.t1 AT2G34390 47.059 153 71 3 1 146 50 199 3.46e-41 140
MS.gene006960.t1 AT3G16240 33.945 218 127 5 2 208 22 233 3.11e-27 105
MS.gene006960.t1 AT3G26520 34.335 233 141 6 2 226 25 253 3.76e-27 105
MS.gene006960.t1 AT5G47450 35.047 214 130 4 2 208 22 233 4.13e-27 104
MS.gene006960.t1 AT4G01470 39.634 164 89 3 53 210 78 237 6.48e-26 101
MS.gene006960.t1 AT4G17340 35.465 172 106 2 53 219 76 247 1.53e-25 100
MS.gene006960.t1 AT1G17810 35.928 167 91 4 53 210 86 245 1.08e-24 99.0
MS.gene006960.t1 AT3G06100 36.170 141 88 2 77 216 41 180 2.31e-24 96.3
MS.gene006960.t1 AT1G73190 35.838 173 95 4 53 216 86 251 5.59e-24 97.1
MS.gene006960.t1 AT2G36830 36.058 208 118 6 2 199 24 226 1.69e-23 95.5
MS.gene006960.t1 AT2G29870 39.655 116 70 0 116 231 20 135 2.98e-23 91.7
MS.gene006960.t1 AT1G31885 52.564 78 33 2 1 77 45 119 1.39e-22 90.1
MS.gene006960.t1 AT2G16850 32.127 221 136 6 1 208 38 257 2.86e-21 90.1
MS.gene006960.t1 AT2G25810 35.882 170 104 3 50 216 69 236 3.32e-21 89.4
MS.gene006960.t1 AT2G37170 29.185 233 138 6 1 210 39 267 7.13e-20 86.3
MS.gene006960.t1 AT2G37170 29.956 227 132 6 1 204 82 304 8.35e-20 86.7
MS.gene006960.t1 AT3G53420 28.326 233 140 5 1 210 41 269 1.22e-19 85.9
MS.gene006960.t1 AT3G53420 28.326 233 140 5 1 210 41 269 1.22e-19 85.9
MS.gene006960.t1 AT2G37180 29.185 233 138 6 1 210 39 267 1.47e-19 85.5
MS.gene006960.t1 AT1G01620 27.876 226 142 5 2 210 55 276 4.42e-19 84.3
MS.gene006960.t1 AT4G23400 26.549 226 145 5 2 210 56 277 6.20e-19 84.0
MS.gene006960.t1 AT4G35100 30.769 221 139 6 1 208 40 259 6.23e-19 83.6
MS.gene006960.t1 AT4G35100 30.769 221 139 6 1 208 40 259 6.23e-19 83.6
MS.gene006960.t1 AT5G60660 28.326 233 140 5 1 210 41 269 1.14e-18 83.2
MS.gene006960.t1 AT4G00430 29.185 233 151 6 2 221 56 287 1.50e-18 82.8
MS.gene006960.t1 AT3G61430 29.185 233 151 6 2 221 55 286 2.08e-18 82.4
MS.gene006960.t1 AT3G61430 29.185 233 151 6 2 221 55 286 2.08e-18 82.4
MS.gene006960.t1 AT2G39010 29.075 227 134 5 1 204 40 262 4.57e-18 81.3
MS.gene006960.t1 AT1G01620 26.699 206 131 4 21 210 3 204 1.26e-17 79.0
MS.gene006960.t1 AT2G45960 28.755 233 152 6 2 221 55 286 2.02e-17 79.7
MS.gene006960.t1 AT3G54820 26.609 233 144 5 1 210 40 268 2.40e-16 76.6
MS.gene006960.t1 AT3G47440 29.630 216 138 6 2 208 26 236 4.51e-15 72.8
MS.gene006960.t1 AT2G45960 27.461 193 130 4 2 184 55 247 2.85e-11 62.4
MS.gene006960.t1 AT2G45960 26.471 204 140 4 2 195 55 258 3.01e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCACATCTACAAAGGATTT+TGG 0.135458 4.4:+26983749 MS.gene006960:CDS
ATGAACCCAGCCAGAACTTT+TGG 0.245283 4.4:+26983712 MS.gene006960:CDS
CCCTTTGTTGGTGCTATATT+AGG 0.248716 4.4:+26983787 MS.gene006960:CDS
ATGATCTTGGTTTATTCTCT+TGG 0.315998 4.4:+26982778 MS.gene006960:CDS
TATCAATCAGATTGGAGAAT+TGG 0.321256 4.4:+26983511 MS.gene006960:intron
ACATACTTCTTAATATTTGC+AGG 0.325876 4.4:+26982667 MS.gene006960:CDS
TATTCTCTTGGTCACATCTC+TGG 0.351110 4.4:+26982790 MS.gene006960:CDS
TACAGCAGGCCCAAAAGTTC+TGG 0.354194 4.4:-26983722 None:intergenic
ACCTGTGCAGCAAGATATAA+TGG 0.385059 4.4:-26983053 None:intergenic
CTGTGACTGCTGGATTGAAA+TGG 0.387793 4.4:-26982816 None:intergenic
AAAGGAACAGTAACATTTCC+TGG 0.388672 4.4:+26982727 MS.gene006960:CDS
TATATAGTTGGACCCTTTGT+TGG 0.388837 4.4:+26983775 MS.gene006960:CDS
GTAGTAGACGTCTTCATTGC+TGG 0.394702 4.4:+26983560 MS.gene006960:CDS
TCGTCATTTCCCGCTTAAAC+AGG 0.397872 4.4:+26982855 MS.gene006960:CDS
GGGTCTATCAGTTATGATCT+TGG 0.398096 4.4:+26982765 MS.gene006960:CDS
CCCCATGTTACACAAATTCC+AGG 0.411162 4.4:-26982745 None:intergenic
TTCCTGGAATTTGTGTAACA+TGG 0.464453 4.4:+26982743 MS.gene006960:CDS
CTAGGATCTGTCCTTGCTAG+TGG 0.477384 4.4:+26983077 MS.gene006960:CDS
AATCAGATTGGAGAATTGGC+AGG 0.484184 4.4:+26983515 MS.gene006960:intron
ATTGCATGCAGCCCTGTGTC+AGG 0.484373 4.4:+26983682 MS.gene006960:intron
TCCTGGAATTTGTGTAACAT+GGG 0.487395 4.4:+26982744 MS.gene006960:CDS
GCCATTATATCTTGCTGCAC+AGG 0.493573 4.4:+26983052 MS.gene006960:CDS
TATCTTGCTGCACAGGTGCT+AGG 0.498223 4.4:+26983059 MS.gene006960:CDS
GCAGGCCCAAAAGTTCTGGC+TGG 0.505285 4.4:-26983718 None:intergenic
TGTGACTGCTGGATTGAAAT+GGG 0.509378 4.4:-26982815 None:intergenic
TTCATTGATGCACCTGACAC+AGG 0.513121 4.4:-26983694 None:intergenic
CGTAGTGGTTCTGAATAAAG+TGG 0.538283 4.4:+26982696 MS.gene006960:CDS
TGTTGTATCTGCAGTTTCCA+CGG 0.547342 4.4:+26983214 MS.gene006960:CDS
AATAAAGTGGAAGAAACTAA+AGG 0.547480 4.4:+26982709 MS.gene006960:CDS
TAGTAGACGTCTTCATTGCT+GGG 0.549173 4.4:+26983561 MS.gene006960:CDS
CATACTTCTTAATATTTGCA+GGG 0.553842 4.4:+26982668 MS.gene006960:CDS
CAGGTAATAGCAGAGGTGAT+AGG 0.557378 4.4:+26982643 None:intergenic
GCGAAGCTAACTGTGACTGC+TGG 0.557473 4.4:-26982826 None:intergenic
ATATGCATGCCTGTTTAAGC+GGG 0.559351 4.4:-26982864 None:intergenic
TAGATGTGCATCACTACAGC+AGG 0.560341 4.4:-26983736 None:intergenic
TATATGCATGCCTGTTTAAG+CGG 0.569385 4.4:-26982865 None:intergenic
CCTAATATAGCACCAACAAA+GGG 0.576824 4.4:-26983787 None:intergenic
CAGGCCCAAAAGTTCTGGCT+GGG 0.576999 4.4:-26983717 None:intergenic
ACCTAATATAGCACCAACAA+AGG 0.581057 4.4:-26983788 None:intergenic
GGCACAATGTTCCACTAGCA+AGG 0.584967 4.4:-26983088 None:intergenic
ATCAGATTGGAGAATTGGCA+GGG 0.586964 4.4:+26983516 MS.gene006960:intron
GAATTGGCAGGGATTGCTGT+TGG 0.598721 4.4:+26983527 MS.gene006960:CDS
ATTTGCAGGGTGTTGCGTAG+TGG 0.602223 4.4:+26982681 MS.gene006960:CDS
CCTGGAATTTGTGTAACATG+GGG 0.603385 4.4:+26982745 MS.gene006960:CDS
GATTGAATATTAGAACCTGC+TGG 0.604048 4.4:-26983152 None:intergenic
TCATTGATGCACCTGACACA+GGG 0.615117 4.4:-26983693 None:intergenic
CTTCGGCTTAATTCTCTGAG+TGG 0.624993 4.4:-26983847 None:intergenic
ACTAACCGCTCTATTGTCCG+TGG 0.634378 4.4:-26983231 None:intergenic
GTAGTGATGCACATCTACAA+AGG 0.641675 4.4:+26983742 MS.gene006960:CDS
TATGCAACAGGTAATAGCAG+AGG 0.665558 4.4:+26982636 None:intergenic
AGTTTCCACGGACAATAGAG+CGG 0.688862 4.4:+26983226 MS.gene006960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTATATATATAGTTTCAA+TGG + chr4.4:26983599-26983618 MS.gene006960:intron 15.0%
!!! AGGAATTTGAATTAATTTTA+TGG - chr4.4:26983486-26983505 None:intergenic 15.0%
!!! CATTTTGGTATATAAAATTA+GGG + chr4.4:26983291-26983310 MS.gene006960:intron 15.0%
!!! TTGAATTAATTTTATGGAAA+AGG - chr4.4:26983480-26983499 None:intergenic 15.0%
!! TCAAACAAAATTTCTATACT+AGG + chr4.4:26983422-26983441 MS.gene006960:intron 20.0%
!!! GAAATTTTGTTTGAAAATAG+TGG - chr4.4:26983416-26983435 None:intergenic 20.0%
!!! GCATTTTGGTATATAAAATT+AGG + chr4.4:26983290-26983309 MS.gene006960:intron 20.0%
!!! GTAGATGAAAAATCTTTTTT+TGG + chr4.4:26983122-26983141 MS.gene006960:CDS 20.0%
! AAATTGCAGCAGTATATATA+TGG - chr4.4:26983646-26983665 None:intergenic 25.0%
! AATAAAGTGGAAGAAACTAA+AGG + chr4.4:26982709-26982728 MS.gene006960:CDS 25.0%
! ACATACTTCTTAATATTTGC+AGG + chr4.4:26982667-26982686 MS.gene006960:CDS 25.0%
! CAGTAAATACTTACTATTAG+TGG - chr4.4:26983356-26983375 None:intergenic 25.0%
! CATACTTCTTAATATTTGCA+GGG + chr4.4:26982668-26982687 MS.gene006960:CDS 25.0%
! CCAAAATGCAAGAAAATATA+AGG - chr4.4:26983279-26983298 None:intergenic 25.0%
! TTTCATCTACATCAAAAAGA+AGG - chr4.4:26983112-26983131 None:intergenic 25.0%
!! CCTTATATTTTCTTGCATTT+TGG + chr4.4:26983276-26983295 MS.gene006960:intron 25.0%
!!! CATAAATTGTTGATTTTTGC+AGG + chr4.4:26983028-26983047 MS.gene006960:intron 25.0%
ATGATCTTGGTTTATTCTCT+TGG + chr4.4:26982778-26982797 MS.gene006960:CDS 30.0%
CCAATCTGATTGATAAAAAG+AGG - chr4.4:26983506-26983525 None:intergenic 30.0%
TATCAATCAGATTGGAGAAT+TGG + chr4.4:26983511-26983530 MS.gene006960:intron 30.0%
TGCATGCAATTATCAAACTT+AGG - chr4.4:26983672-26983691 None:intergenic 30.0%
! CCTCTTTTTATCAATCAGAT+TGG + chr4.4:26983503-26983522 MS.gene006960:intron 30.0%
!!! GGATTTTGGGTTTATATAGT+TGG + chr4.4:26983763-26983782 MS.gene006960:CDS 30.0%
AAAAGGCTAACATGTGTCTT+AGG - chr4.4:26983463-26983482 None:intergenic 35.0%
AAAGGAACAGTAACATTTCC+TGG + chr4.4:26982727-26982746 MS.gene006960:CDS 35.0%
AAAGGCTAACATGTGTCTTA+GGG - chr4.4:26983462-26983481 None:intergenic 35.0%
ACCTAATATAGCACCAACAA+AGG - chr4.4:26983791-26983810 None:intergenic 35.0%
CCTAATATAGCACCAACAAA+GGG - chr4.4:26983790-26983809 None:intergenic 35.0%
CTTAACGACTGAATTAGAGA+TGG + chr4.4:26982979-26982998 MS.gene006960:intron 35.0%
GATTGAATATTAGAACCTGC+TGG - chr4.4:26983155-26983174 None:intergenic 35.0%
TATATAGTTGGACCCTTTGT+TGG + chr4.4:26983775-26983794 MS.gene006960:CDS 35.0%
TATATGCATGCCTGTTTAAG+CGG - chr4.4:26982868-26982887 None:intergenic 35.0%
TCCTGGAATTTGTGTAACAT+GGG + chr4.4:26982744-26982763 MS.gene006960:CDS 35.0%
TTCCTGGAATTTGTGTAACA+TGG + chr4.4:26982743-26982762 MS.gene006960:CDS 35.0%
! AAGTGTTTGAGACGACAAAA+TGG - chr4.4:26982962-26982981 None:intergenic 35.0%
! AGTGTTTGAGACGACAAAAT+GGG - chr4.4:26982961-26982980 None:intergenic 35.0%
! GCACATCTACAAAGGATTTT+GGG + chr4.4:26983750-26983769 MS.gene006960:CDS 35.0%
! TGCACATCTACAAAGGATTT+TGG + chr4.4:26983749-26983768 MS.gene006960:CDS 35.0%
AATCAGATTGGAGAATTGGC+AGG + chr4.4:26983515-26983534 MS.gene006960:intron 40.0%
ACCTGTGCAGCAAGATATAA+TGG - chr4.4:26983056-26983075 None:intergenic 40.0%
ATATGCATGCCTGTTTAAGC+GGG - chr4.4:26982867-26982886 None:intergenic 40.0%
ATCAGATTGGAGAATTGGCA+GGG + chr4.4:26983516-26983535 MS.gene006960:intron 40.0%
CATGCTAATATGTGCCCTAA+GGG + chr4.4:26983315-26983334 MS.gene006960:intron 40.0%
CCTGGAATTTGTGTAACATG+GGG + chr4.4:26982745-26982764 MS.gene006960:CDS 40.0%
GGGTCTATCAGTTATGATCT+TGG + chr4.4:26982765-26982784 MS.gene006960:CDS 40.0%
GTAGTGATGCACATCTACAA+AGG + chr4.4:26983742-26983761 MS.gene006960:CDS 40.0%
TAGTAGACGTCTTCATTGCT+GGG + chr4.4:26983561-26983580 MS.gene006960:CDS 40.0%
TATTCTCTTGGTCACATCTC+TGG + chr4.4:26982790-26982809 MS.gene006960:CDS 40.0%
TCATGCTAATATGTGCCCTA+AGG + chr4.4:26983314-26983333 MS.gene006960:intron 40.0%
TGTGACTGCTGGATTGAAAT+GGG - chr4.4:26982818-26982837 None:intergenic 40.0%
TGTTGTATCTGCAGTTTCCA+CGG + chr4.4:26983214-26983233 MS.gene006960:CDS 40.0%
!! CCCTTTGTTGGTGCTATATT+AGG + chr4.4:26983787-26983806 MS.gene006960:CDS 40.0%
!! CGTAGTGGTTCTGAATAAAG+TGG + chr4.4:26982696-26982715 MS.gene006960:CDS 40.0%
!! TTTTGAGGAAGCTTGAGCTT+CGG - chr4.4:26983867-26983886 None:intergenic 40.0%
!!! GTGTCTCTAGAGCAAGTTTT+CGG + chr4.4:26983887-26983906 MS.gene006960:CDS 40.0%
!!! TTGCTCTAGAGACACTTTTG+AGG - chr4.4:26983882-26983901 None:intergenic 40.0%
!!! TTTTTTGGAACAGTACCAGC+AGG + chr4.4:26983137-26983156 MS.gene006960:CDS 40.0%
AGTTTCCACGGACAATAGAG+CGG + chr4.4:26983226-26983245 MS.gene006960:CDS 45.0%
CCCCATGTTACACAAATTCC+AGG - chr4.4:26982748-26982767 None:intergenic 45.0%
CTGTGACTGCTGGATTGAAA+TGG - chr4.4:26982819-26982838 None:intergenic 45.0%
CTTCGGCTTAATTCTCTGAG+TGG - chr4.4:26983850-26983869 None:intergenic 45.0%
GCCATTATATCTTGCTGCAC+AGG + chr4.4:26983052-26983071 MS.gene006960:CDS 45.0%
GTAGTAGACGTCTTCATTGC+TGG + chr4.4:26983560-26983579 MS.gene006960:CDS 45.0%
TAGATGTGCATCACTACAGC+AGG - chr4.4:26983739-26983758 None:intergenic 45.0%
TCATTGATGCACCTGACACA+GGG - chr4.4:26983696-26983715 None:intergenic 45.0%
TCGTCATTTCCCGCTTAAAC+AGG + chr4.4:26982855-26982874 MS.gene006960:CDS 45.0%
TTCATTGATGCACCTGACAC+AGG - chr4.4:26983697-26983716 None:intergenic 45.0%
! AATCCCTAACTTGTGCCCTT+AGG - chr4.4:26983333-26983352 None:intergenic 45.0%
! ATCCCTAACTTGTGCCCTTA+GGG - chr4.4:26983332-26983351 None:intergenic 45.0%
! ATGAACCCAGCCAGAACTTT+TGG + chr4.4:26983712-26983731 MS.gene006960:CDS 45.0%
! TGAACCCAGCCAGAACTTTT+GGG + chr4.4:26983713-26983732 MS.gene006960:CDS 45.0%
!!! CTCTAGAGCAAGTTTTCGGT+AGG + chr4.4:26983891-26983910 MS.gene006960:CDS 45.0%
ACTAACCGCTCTATTGTCCG+TGG - chr4.4:26983234-26983253 None:intergenic 50.0%
CTAGGATCTGTCCTTGCTAG+TGG + chr4.4:26983077-26983096 MS.gene006960:CDS 50.0%
GAATTGGCAGGGATTGCTGT+TGG + chr4.4:26983527-26983546 MS.gene006960:CDS 50.0%
GGCACAATGTTCCACTAGCA+AGG - chr4.4:26983091-26983110 None:intergenic 50.0%
TACAGCAGGCCCAAAAGTTC+TGG - chr4.4:26983725-26983744 None:intergenic 50.0%
TATCTTGCTGCACAGGTGCT+AGG + chr4.4:26983059-26983078 MS.gene006960:CDS 50.0%
! TGCCCTAAGGGCACAAGTTA+GGG + chr4.4:26983327-26983346 MS.gene006960:intron 50.0%
!! ATTTGCAGGGTGTTGCGTAG+TGG + chr4.4:26982681-26982700 MS.gene006960:CDS 50.0%
ATTGCATGCAGCCCTGTGTC+AGG + chr4.4:26983682-26983701 MS.gene006960:intron 55.0%
GCGAAGCTAACTGTGACTGC+TGG - chr4.4:26982829-26982848 None:intergenic 55.0%
! CAGGCCCAAAAGTTCTGGCT+GGG - chr4.4:26983720-26983739 None:intergenic 55.0%
! GTGCCCTAAGGGCACAAGTT+AGG + chr4.4:26983326-26983345 MS.gene006960:intron 55.0%
! GCAGGCCCAAAAGTTCTGGC+TGG - chr4.4:26983721-26983740 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 26982646 26983912 26982646 ID=MS.gene006960
chr4.4 mRNA 26982646 26983912 26982646 ID=MS.gene006960.t1;Parent=MS.gene006960
chr4.4 exon 26982646 26982876 26982646 ID=MS.gene006960.t1.exon1;Parent=MS.gene006960.t1
chr4.4 CDS 26982646 26982876 26982646 ID=cds.MS.gene006960.t1;Parent=MS.gene006960.t1
chr4.4 exon 26983050 26983247 26983050 ID=MS.gene006960.t1.exon2;Parent=MS.gene006960.t1
chr4.4 CDS 26983050 26983247 26983050 ID=cds.MS.gene006960.t1;Parent=MS.gene006960.t1
chr4.4 exon 26983521 26983582 26983521 ID=MS.gene006960.t1.exon3;Parent=MS.gene006960.t1
chr4.4 CDS 26983521 26983582 26983521 ID=cds.MS.gene006960.t1;Parent=MS.gene006960.t1
chr4.4 exon 26983693 26983912 26983693 ID=MS.gene006960.t1.exon4;Parent=MS.gene006960.t1
chr4.4 CDS 26983693 26983912 26983693 ID=cds.MS.gene006960.t1;Parent=MS.gene006960.t1
Gene Sequence

>MS.gene006960

GTAATAGCAGAGGTGATAGGAACATACTTCTTAATATTTGCAGGGTGTTGCGTAGTGGTTCTGAATAAAGTGGAAGAAACTAAAGGAACAGTAACATTTCCTGGAATTTGTGTAACATGGGGTCTATCAGTTATGATCTTGGTTTATTCTCTTGGTCACATCTCTGGTGCCCATTTCAATCCAGCAGTCACAGTTAGCTTCGCAATCTATCGTCATTTCCCGCTTAAACAGGCATGCATATACTTATGACAATTGATCTACTCAGCTCACATTGTTATAAAAAGTTGAATTAACGAAAAAAATAATTACTCTCCCATTTTGTCGTCTCAAACACTTAACGACTGAATTAGAGATGGATTTCTTGTTTTTCGAACATATGTAACATAAATTGTTGATTTTTGCAGGTGCCATTATATCTTGCTGCACAGGTGCTAGGATCTGTCCTTGCTAGTGGAACATTGTGCCTTCTTTTTGATGTAGATGAAAAATCTTTTTTTGGAACAGTACCAGCAGGTTCTAATATTCAATCTCTTGTCTTTGAGATACTCACATCATTTCTCTTGATGTTTGTTGTATCTGCAGTTTCCACGGACAATAGAGCGGTTAGTATACTCAGTTTAGTTTAATTTACCTTATATTTTCTTGCATTTTGGTATATAAAATTAGGGTCATGCTAATATGTGCCCTAAGGGCACAAGTTAGGGATTCCACTAATAGTAAGTATTTACTGAGAAAACATTAATTTTCAATTTTTTAATAATAAATTGCCACTATTTTCAAACAAAATTTCTATACTAGGTTCATTCACATGTGCCCTAAGACACATGTTAGCCTTTTCCATAAAATTAATTCAAATTCCTCTTTTTATCAATCAGATTGGAGAATTGGCAGGGATTGCTGTTGGCATGACAATCGTAGTAGACGTCTTCATTGCTGGGTAATTTCAACACTATTACTATATATATAGTTTCAATGGTTTTACTTATCATAATGAAGTCCATATATATACTGCTGCAATTTAATCCTAAGTTTGATAATTGCATGCAGCCCTGTGTCAGGTGCATCAATGAACCCAGCCAGAACTTTTGGGCCTGCTGTAGTGATGCACATCTACAAAGGATTTTGGGTTTATATAGTTGGACCCTTTGTTGGTGCTATATTAGGTGCATCAGCTTACAACTTGATTAGATTTACTGATAAACCACTCAGAGAATTAAGCCGAAGCTCAAGCTTCCTCAAAAGTGTCTCTAGAGCAAGTTTTCGGTAG
Protein sequence

>MS.gene006960.t1

VIAEVIGTYFLIFAGCCVVVLNKVEETKGTVTFPGICVTWGLSVMILVYSLGHISGAHFNPAVTVSFAIYRHFPLKQVPLYLAAQVLGSVLASGTLCLLFDVDEKSFFGTVPAGSNIQSLVFEILTSFLLMFVVSAVSTDNRAIGELAGIAVGMTIVVDVFIAGPVSGASMNPARTFGPAVVMHIYKGFWVYIVGPFVGAILGASAYNLIRFTDKPLRELSRSSSFLKSVSRASFR