AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059531

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059531.t1 MTR_4g084880 97.736 265 5 1 1 264 9 273 0.0 518
MS.gene059531.t1 MTR_8g087720 58.103 253 104 1 5 257 16 266 1.75e-108 315
MS.gene059531.t1 MTR_3g008920 63.203 231 82 1 17 247 16 243 8.53e-107 310
MS.gene059531.t1 MTR_5g079570 63.158 228 81 1 17 244 30 254 9.46e-107 310
MS.gene059531.t1 MTR_8g087710 56.202 258 108 2 10 264 19 274 3.11e-105 306
MS.gene059531.t1 MTR_2g017570 57.787 244 101 1 21 264 32 273 2.22e-103 301
MS.gene059531.t1 MTR_2g017590 57.500 240 100 1 23 262 32 269 8.23e-103 300
MS.gene059531.t1 MTR_2g017610 57.377 244 102 1 21 264 32 273 1.46e-102 300
MS.gene059531.t1 MTR_2g017660 53.333 240 110 1 5 244 17 254 9.93e-96 282
MS.gene059531.t1 MTR_2g017620 56.410 234 100 1 23 256 20 251 9.75e-93 274
MS.gene059531.t1 MTR_2g017620 56.410 234 100 1 23 256 34 265 1.47e-92 274
MS.gene059531.t1 MTR_7g010650 49.345 229 112 3 27 255 37 261 2.59e-76 233
MS.gene059531.t1 MTR_2g017620 57.609 184 78 0 73 256 1 184 2.07e-75 228
MS.gene059531.t1 MTR_8g087720 57.979 188 77 1 5 192 16 201 2.31e-73 223
MS.gene059531.t1 MTR_4g006730 46.768 263 126 4 1 260 54 305 2.07e-70 219
MS.gene059531.t1 MTR_2g104940 46.025 239 120 3 25 260 78 310 1.01e-68 215
MS.gene059531.t1 MTR_4g006630 48.416 221 111 2 40 260 1 218 1.47e-64 201
MS.gene059531.t1 MTR_4g006730 48.416 221 111 2 40 260 1 218 2.67e-64 200
MS.gene059531.t1 MTR_8g087720 56.548 168 71 1 5 172 16 181 1.12e-62 196
MS.gene059531.t1 MTR_1g097840 43.882 237 118 4 23 250 70 300 4.24e-61 195
MS.gene059531.t1 MTR_5g063930 41.739 230 124 4 26 251 36 259 7.68e-59 188
MS.gene059531.t1 MTR_4g006730 45.778 225 96 6 1 222 54 255 1.11e-46 157
MS.gene059531.t1 MTR_1g070385 37.209 215 132 2 29 243 64 275 1.72e-45 163
MS.gene059531.t1 MTR_7g010650 47.260 146 74 2 27 172 37 179 1.16e-41 142
MS.gene059531.t1 MTR_1g097840 43.353 173 89 3 23 192 70 236 6.80e-38 134
MS.gene059531.t1 MTR_5g012810 41.714 175 92 5 81 248 76 247 4.31e-27 105
MS.gene059531.t1 MTR_4g063090 39.080 174 96 3 81 248 78 247 6.36e-27 105
MS.gene059531.t1 MTR_2g101370 33.333 228 133 6 23 238 16 236 3.21e-26 103
MS.gene059531.t1 MTR_4g006650 46.939 147 60 4 28 171 5 136 4.15e-26 104
MS.gene059531.t1 MTR_1g077540 31.696 224 148 3 26 244 20 243 1.34e-25 102
MS.gene059531.t1 MTR_1g115405 34.454 238 139 5 26 252 20 251 3.43e-25 100
MS.gene059531.t1 MTR_8g013680 32.751 229 147 4 26 247 18 246 8.85e-25 99.4
MS.gene059531.t1 MTR_1g006490 33.182 220 130 6 26 236 18 229 6.86e-24 97.1
MS.gene059531.t1 MTR_6g069040 30.088 226 149 3 26 244 20 243 2.59e-23 95.5
MS.gene059531.t1 MTR_7g023350 31.984 247 152 5 15 252 12 251 2.86e-22 92.8
MS.gene059531.t1 MTR_1g006590 32.018 228 142 6 26 245 18 240 3.79e-21 89.7
MS.gene059531.t1 MTR_3g070210 27.876 226 143 5 30 238 58 280 4.20e-21 90.5
MS.gene059531.t1 MTR_2g094270 31.092 238 136 8 25 238 38 271 5.67e-21 90.1
MS.gene059531.t1 MTR_3g118010 29.515 227 138 6 27 236 37 258 2.45e-20 88.2
MS.gene059531.t1 MTR_5g082070 27.350 234 149 6 30 246 58 287 5.43e-20 87.4
MS.gene059531.t1 MTR_7g101190 28.346 254 152 8 27 255 39 287 1.72e-19 85.9
MS.gene059531.t1 MTR_5g010150 25.984 254 154 6 2 238 41 277 6.58e-19 84.3
MS.gene059531.t1 MTR_4g059390 31.771 192 111 5 63 238 82 269 7.08e-19 84.3
MS.gene059531.t1 MTR_7g103030 27.711 249 160 6 17 252 8 249 1.31e-18 82.8
MS.gene059531.t1 MTR_8g098375 25.100 251 160 5 2 238 41 277 6.95e-18 81.6
MS.gene059531.t1 MTR_4g415300 28.251 223 145 6 30 238 59 280 7.88e-17 78.6
MS.gene059531.t1 MTR_1g095070 29.508 183 109 4 72 238 25 203 6.23e-16 75.1
MS.gene059531.t1 MTR_3g070210 26.904 197 127 4 30 212 58 251 1.09e-15 75.5
MS.gene059531.t1 MTR_2g060360 29.064 203 112 4 75 264 70 253 2.85e-15 73.9
MS.gene059531.t1 MTR_2g094270 28.972 214 127 7 25 217 38 247 3.37e-15 73.9
MS.gene059531.t1 MTR_1g095070 28.947 152 91 3 72 210 25 172 5.00e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059531.t1 AT5G37820 64.368 261 90 2 1 260 16 274 1.14e-120 346
MS.gene059531.t1 AT5G37820 64.368 261 90 2 1 260 52 310 4.42e-120 346
MS.gene059531.t1 AT5G37810 65.000 260 88 2 1 259 16 273 2.13e-118 340
MS.gene059531.t1 AT4G18910 59.839 249 91 2 23 264 48 294 2.49e-105 307
MS.gene059531.t1 AT4G19030 55.061 247 102 2 23 262 51 295 7.28e-96 283
MS.gene059531.t1 AT1G31885 49.020 255 118 4 5 250 20 271 8.99e-81 246
MS.gene059531.t1 AT1G31885 51.818 220 95 3 39 250 1 217 4.47e-75 229
MS.gene059531.t1 AT2G34390 45.902 244 122 3 23 259 44 284 1.48e-70 219
MS.gene059531.t1 AT2G34390 45.607 239 120 3 28 259 7 242 1.65e-68 212
MS.gene059531.t1 AT4G18910 59.322 177 63 2 23 192 48 222 3.63e-67 208
MS.gene059531.t1 AT1G80760 44.488 254 129 4 1 252 59 302 2.29e-66 209
MS.gene059531.t1 AT4G19030 53.409 176 73 2 23 191 51 224 4.66e-61 192
MS.gene059531.t1 AT4G10380 43.983 241 119 5 19 250 71 304 3.96e-60 192
MS.gene059531.t1 AT4G19030 52.229 157 66 2 23 172 51 205 6.91e-52 169
MS.gene059531.t1 AT2G34390 46.893 177 84 3 23 192 44 217 4.28e-49 161
MS.gene059531.t1 AT3G06100 38.636 220 131 3 26 244 45 261 5.16e-48 160
MS.gene059531.t1 AT3G06100 38.889 216 128 3 30 244 1 213 1.18e-47 158
MS.gene059531.t1 AT2G34390 45.977 174 79 5 10 174 32 199 1.05e-43 148
MS.gene059531.t1 AT5G47450 34.335 233 144 4 11 236 3 233 1.87e-28 109
MS.gene059531.t1 AT3G26520 34.783 230 138 6 26 247 21 246 1.84e-27 106
MS.gene059531.t1 AT3G16240 33.333 222 131 5 26 236 18 233 3.23e-27 105
MS.gene059531.t1 AT4G01470 39.634 164 89 3 81 238 78 237 6.57e-26 102
MS.gene059531.t1 AT4G17340 35.465 172 106 2 81 247 76 247 2.32e-25 101
MS.gene059531.t1 AT1G17810 30.556 252 149 8 6 238 1 245 2.67e-25 101
MS.gene059531.t1 AT1G31885 46.602 103 50 3 5 105 20 119 6.81e-25 97.1
MS.gene059531.t1 AT3G06100 36.170 141 88 2 105 244 41 180 3.25e-24 96.7
MS.gene059531.t1 AT1G73190 30.233 258 154 8 6 244 1 251 3.56e-24 98.2
MS.gene059531.t1 AT2G36830 35.377 212 122 6 26 227 20 226 5.64e-24 97.4
MS.gene059531.t1 AT2G29870 39.655 116 70 0 144 259 20 135 6.06e-23 91.7
MS.gene059531.t1 AT2G16850 31.839 223 138 6 27 236 36 257 1.49e-21 91.7
MS.gene059531.t1 AT2G25810 31.646 237 156 4 11 244 3 236 2.04e-21 90.5
MS.gene059531.t1 AT2G37170 28.571 238 143 6 24 238 34 267 2.51e-20 88.2
MS.gene059531.t1 AT2G37170 28.571 238 143 6 24 238 77 310 3.34e-20 88.6
MS.gene059531.t1 AT2G37180 28.571 238 143 6 24 238 34 267 5.21e-20 87.4
MS.gene059531.t1 AT3G53420 28.085 235 142 5 27 238 39 269 5.42e-20 87.4
MS.gene059531.t1 AT3G53420 28.085 235 142 5 27 238 39 269 5.42e-20 87.4
MS.gene059531.t1 AT4G23400 25.197 254 169 5 2 238 28 277 2.06e-19 85.9
MS.gene059531.t1 AT1G01620 27.876 226 142 5 30 238 55 276 2.64e-19 85.5
MS.gene059531.t1 AT4G35100 30.493 223 141 6 27 236 38 259 3.74e-19 85.1
MS.gene059531.t1 AT4G35100 30.493 223 141 6 27 236 38 259 3.74e-19 85.1
MS.gene059531.t1 AT5G60660 27.848 237 144 5 25 238 37 269 5.54e-19 84.7
MS.gene059531.t1 AT4G00430 29.185 233 151 6 30 249 56 287 9.70e-19 84.0
MS.gene059531.t1 AT3G61430 29.185 233 151 6 30 249 55 286 1.60e-18 83.2
MS.gene059531.t1 AT3G61430 29.185 233 151 6 30 249 55 286 1.60e-18 83.2
MS.gene059531.t1 AT2G39010 28.085 235 142 5 27 238 38 268 1.82e-18 83.2
MS.gene059531.t1 AT2G45960 27.897 233 154 6 30 249 55 286 1.39e-17 80.9
MS.gene059531.t1 AT1G01620 26.699 206 131 4 49 238 3 204 1.58e-17 79.3
MS.gene059531.t1 AT3G54820 26.383 235 146 5 27 238 38 268 1.21e-16 78.2
MS.gene059531.t1 AT3G47440 29.630 216 138 6 30 236 26 236 6.92e-15 72.8
MS.gene059531.t1 AT2G45960 25.490 204 142 4 30 223 55 258 1.73e-11 63.5
MS.gene059531.t1 AT2G45960 27.461 193 130 4 30 212 55 247 1.80e-11 63.2

Find 55 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCACATCTACAAAGGATTT+TGG 0.135458 4.4:+26895194 MS.gene059531:CDS
ATGAACCCAGCCAGAACTTT+TGG 0.245283 4.4:+26895157 MS.gene059531:CDS
CCCTTTGTTGGTGCTATATT+AGG 0.248716 4.4:+26895232 MS.gene059531:CDS
ATGATCTTGGTTTATTCTCT+TGG 0.315998 4.4:+26894223 MS.gene059531:CDS
TATCAATCAGATTGGAGAAT+TGG 0.321256 4.4:+26894956 MS.gene059531:intron
ACATACTTCTTAATATTTGC+AGG 0.325876 4.4:+26894112 MS.gene059531:CDS
TATTCTCTTGGTCACATCTC+TGG 0.351110 4.4:+26894235 MS.gene059531:CDS
TACAGCAGGCCCAAAAGTTC+TGG 0.354194 4.4:-26895167 None:intergenic
ACCTGTGCAGCAAGATATAA+TGG 0.385059 4.4:-26894499 None:intergenic
CTGTGACTGCTGGATTGAAA+TGG 0.387793 4.4:-26894261 None:intergenic
AAAGGAACAGTAACATTTCC+TGG 0.388672 4.4:+26894172 MS.gene059531:CDS
TATATAGTTGGACCCTTTGT+TGG 0.388837 4.4:+26895220 MS.gene059531:CDS
GTAGTAGACGTCTTCATTGC+TGG 0.394702 4.4:+26895005 MS.gene059531:CDS
TCGTCATTTCCCGCTTAAAC+AGG 0.397872 4.4:+26894300 MS.gene059531:CDS
GGGTCTATCAGTTATGATCT+TGG 0.398096 4.4:+26894210 MS.gene059531:CDS
CCCCATGTTACACAAATTCC+AGG 0.411162 4.4:-26894190 None:intergenic
AATGGAAGAAGGTGATCTTA+AGG 0.450995 4.4:+26893858 None:intergenic
TTCCTGGAATTTGTGTAACA+TGG 0.464453 4.4:+26894188 MS.gene059531:CDS
TGTATGATTTGAACTACCTC+AGG 0.467934 4.4:-26893916 None:intergenic
CTAGGATCTGTCCTTGCTAG+TGG 0.477384 4.4:+26894523 MS.gene059531:CDS
AATCAGATTGGAGAATTGGC+AGG 0.484184 4.4:+26894960 MS.gene059531:intron
ATTGCATGCAGCCCTGTGTC+AGG 0.484373 4.4:+26895127 MS.gene059531:intron
TCCTGGAATTTGTGTAACAT+GGG 0.487395 4.4:+26894189 MS.gene059531:CDS
GCCATTATATCTTGCTGCAC+AGG 0.493573 4.4:+26894498 MS.gene059531:CDS
TATCTTGCTGCACAGGTGCT+AGG 0.498223 4.4:+26894505 MS.gene059531:CDS
GCAGGCCCAAAAGTTCTGGC+TGG 0.505285 4.4:-26895163 None:intergenic
TGTGACTGCTGGATTGAAAT+GGG 0.509378 4.4:-26894260 None:intergenic
GGTAGTTCAAATCATACAAA+AGG 0.511595 4.4:+26893921 MS.gene059531:CDS
TTCATTGATGCACCTGACAC+AGG 0.513121 4.4:-26895139 None:intergenic
CGTAGTGGTTCTGAATAAAG+TGG 0.538283 4.4:+26894141 MS.gene059531:CDS
TGTTGTATCTGCAGTTTCCA+CGG 0.547342 4.4:+26894660 MS.gene059531:CDS
AATAAAGTGGAAGAAACTAA+AGG 0.547480 4.4:+26894154 MS.gene059531:CDS
TAGTAGACGTCTTCATTGCT+GGG 0.549173 4.4:+26895006 MS.gene059531:CDS
GAGATCACGAAAATGGAAGA+AGG 0.552927 4.4:+26893847 None:intergenic
CATACTTCTTAATATTTGCA+GGG 0.553842 4.4:+26894113 MS.gene059531:CDS
CAGGTAATAGCAGAGGTGAT+AGG 0.557378 4.4:+26894088 MS.gene059531:intron
GCGAAGCTAACTGTGACTGC+TGG 0.557473 4.4:-26894271 None:intergenic
ATATGCATGCCTGTTTAAGC+GGG 0.559351 4.4:-26894309 None:intergenic
TAGATGTGCATCACTACAGC+AGG 0.560341 4.4:-26895181 None:intergenic
TATATGCATGCCTGTTTAAG+CGG 0.569385 4.4:-26894310 None:intergenic
CCTAATATAGCACCAACAAA+GGG 0.576824 4.4:-26895232 None:intergenic
CAGGCCCAAAAGTTCTGGCT+GGG 0.576999 4.4:-26895162 None:intergenic
ACCTAATATAGCACCAACAA+AGG 0.581057 4.4:-26895233 None:intergenic
GGCACAATGTTCCACTAGCA+AGG 0.584967 4.4:-26894534 None:intergenic
ATCAGATTGGAGAATTGGCA+GGG 0.586964 4.4:+26894961 MS.gene059531:intron
GAATTGGCAGGGATTGCTGT+TGG 0.598721 4.4:+26894972 MS.gene059531:CDS
ATTTGCAGGGTGTTGCGTAG+TGG 0.602223 4.4:+26894126 MS.gene059531:CDS
CCTGGAATTTGTGTAACATG+GGG 0.603385 4.4:+26894190 MS.gene059531:CDS
GATTGAATATTAGAACCTGC+TGG 0.604048 4.4:-26894598 None:intergenic
TCATTGATGCACCTGACACA+GGG 0.615117 4.4:-26895138 None:intergenic
CTTCGGCTTAATTCTCTGAG+TGG 0.624993 4.4:-26895292 None:intergenic
ACTAACCGCTCTATTGTCCG+TGG 0.634378 4.4:-26894677 None:intergenic
GTAGTGATGCACATCTACAA+AGG 0.641675 4.4:+26895187 MS.gene059531:CDS
TATGCAACAGGTAATAGCAG+AGG 0.665558 4.4:+26894081 MS.gene059531:intron
AGTTTCCACGGACAATAGAG+CGG 0.688862 4.4:+26894672 MS.gene059531:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATTAATGTGATTGTA+TGG - chr4.4:26893975-26893994 None:intergenic 15.0%
!! TACTATATATATAGTTTCAA+TGG + chr4.4:26895044-26895063 MS.gene059531:intron 15.0%
!!! AAATTTTGTTTTGAAAATAG+TGG - chr4.4:26894860-26894879 None:intergenic 15.0%
!!! AGGAATTTGAATTAATTTTA+TGG - chr4.4:26894931-26894950 None:intergenic 15.0%
!!! CATTTTGGTATATAAAATTA+GGG + chr4.4:26894737-26894756 MS.gene059531:intron 15.0%
!!! TTGAATTAATTTTATGGAAA+AGG - chr4.4:26894925-26894944 None:intergenic 15.0%
!! CAAAACAAAATTTCTATACT+AGG + chr4.4:26894867-26894886 MS.gene059531:intron 20.0%
!!! GCATTTTGGTATATAAAATT+AGG + chr4.4:26894736-26894755 MS.gene059531:intron 20.0%
!!! GTAGATGAAAAATCTTTTTT+TGG + chr4.4:26894568-26894587 MS.gene059531:CDS 20.0%
! AAAAATGAAATGTATGCAAC+AGG + chr4.4:26894069-26894088 MS.gene059531:intron 25.0%
! AAATTGCAGCAGTATATATA+TGG - chr4.4:26895091-26895110 None:intergenic 25.0%
! AATAAAGTGGAAGAAACTAA+AGG + chr4.4:26894154-26894173 MS.gene059531:CDS 25.0%
! ACATACTTCTTAATATTTGC+AGG + chr4.4:26894112-26894131 MS.gene059531:CDS 25.0%
! CATACTTCTTAATATTTGCA+GGG + chr4.4:26894113-26894132 MS.gene059531:CDS 25.0%
! CCAAAATGCAAGAAAATATA+AGG - chr4.4:26894725-26894744 None:intergenic 25.0%
! TCAGTAATACTTACTATTAG+TGG - chr4.4:26894802-26894821 None:intergenic 25.0%
! TTTCATCTACATCAAAAAGA+AGG - chr4.4:26894558-26894577 None:intergenic 25.0%
!! CCTTATATTTTCTTGCATTT+TGG + chr4.4:26894722-26894741 MS.gene059531:intron 25.0%
!!! CATAAATTGTTGATTTTTGC+AGG + chr4.4:26894474-26894493 MS.gene059531:intron 25.0%
AATGTGATTGTATGGTACAA+TGG - chr4.4:26893967-26893986 None:intergenic 30.0%
ATATGTGTACAATGGTACAA+TGG - chr4.4:26894020-26894039 None:intergenic 30.0%
ATGATCTTGGTTTATTCTCT+TGG + chr4.4:26894223-26894242 MS.gene059531:CDS 30.0%
CACAAACAAAAAGTGAATCA+GGG + chr4.4:26893881-26893900 MS.gene059531:CDS 30.0%
CCAATCTGATTGATAAAAAG+AGG - chr4.4:26894951-26894970 None:intergenic 30.0%
GGTAGTTCAAATCATACAAA+AGG + chr4.4:26893921-26893940 MS.gene059531:CDS 30.0%
TATCAATCAGATTGGAGAAT+TGG + chr4.4:26894956-26894975 MS.gene059531:intron 30.0%
TGACAAAGATATGTGTACAA+TGG - chr4.4:26894028-26894047 None:intergenic 30.0%
TGCATGCAATTATCAAACTT+AGG - chr4.4:26895117-26895136 None:intergenic 30.0%
! CCTCTTTTTATCAATCAGAT+TGG + chr4.4:26894948-26894967 MS.gene059531:intron 30.0%
!!! GGATTTTGGGTTTATATAGT+TGG + chr4.4:26895208-26895227 MS.gene059531:CDS 30.0%
AAAAGGCTAACATGTGTCTT+AGG - chr4.4:26894908-26894927 None:intergenic 35.0%
AAAGGAACAGTAACATTTCC+TGG + chr4.4:26894172-26894191 MS.gene059531:CDS 35.0%
AAAGGCTAACATGTGTCTTA+GGG - chr4.4:26894907-26894926 None:intergenic 35.0%
ACCTAATATAGCACCAACAA+AGG - chr4.4:26895236-26895255 None:intergenic 35.0%
CCTAATATAGCACCAACAAA+GGG - chr4.4:26895235-26895254 None:intergenic 35.0%
CTTAACGACTGAATTAGAGA+TGG + chr4.4:26894425-26894444 MS.gene059531:intron 35.0%
GATTGAATATTAGAACCTGC+TGG - chr4.4:26894601-26894620 None:intergenic 35.0%
GCACAAACAAAAAGTGAATC+AGG + chr4.4:26893880-26893899 MS.gene059531:CDS 35.0%
TATATAGTTGGACCCTTTGT+TGG + chr4.4:26895220-26895239 MS.gene059531:CDS 35.0%
TATATGCATGCCTGTTTAAG+CGG - chr4.4:26894313-26894332 None:intergenic 35.0%
TCCTGGAATTTGTGTAACAT+GGG + chr4.4:26894189-26894208 MS.gene059531:CDS 35.0%
TGTATGATTTGAACTACCTC+AGG - chr4.4:26893919-26893938 None:intergenic 35.0%
TTCCTGGAATTTGTGTAACA+TGG + chr4.4:26894188-26894207 MS.gene059531:CDS 35.0%
! AAAAGTGAATCAGGGTTTTG+TGG + chr4.4:26893889-26893908 MS.gene059531:CDS 35.0%
! AAGTGTTTGAGACGACAAAA+TGG - chr4.4:26894408-26894427 None:intergenic 35.0%
! AGTGTTTGAGACGACAAAAT+GGG - chr4.4:26894407-26894426 None:intergenic 35.0%
! GCACATCTACAAAGGATTTT+GGG + chr4.4:26895195-26895214 MS.gene059531:CDS 35.0%
! TGCACATCTACAAAGGATTT+TGG + chr4.4:26895194-26895213 MS.gene059531:CDS 35.0%
AATCAGATTGGAGAATTGGC+AGG + chr4.4:26894960-26894979 MS.gene059531:intron 40.0%
ACCTGTGCAGCAAGATATAA+TGG - chr4.4:26894502-26894521 None:intergenic 40.0%
ATATGCATGCCTGTTTAAGC+GGG - chr4.4:26894312-26894331 None:intergenic 40.0%
ATCAGATTGGAGAATTGGCA+GGG + chr4.4:26894961-26894980 MS.gene059531:intron 40.0%
CATGCTAATATGTGCCCTAA+GGG + chr4.4:26894761-26894780 MS.gene059531:intron 40.0%
CCTGGAATTTGTGTAACATG+GGG + chr4.4:26894190-26894209 MS.gene059531:CDS 40.0%
GGGTCTATCAGTTATGATCT+TGG + chr4.4:26894210-26894229 MS.gene059531:CDS 40.0%
GTAGTGATGCACATCTACAA+AGG + chr4.4:26895187-26895206 MS.gene059531:CDS 40.0%
TAGTAGACGTCTTCATTGCT+GGG + chr4.4:26895006-26895025 MS.gene059531:CDS 40.0%
TATGCAACAGGTAATAGCAG+AGG + chr4.4:26894081-26894100 MS.gene059531:intron 40.0%
TATTCTCTTGGTCACATCTC+TGG + chr4.4:26894235-26894254 MS.gene059531:CDS 40.0%
TCATGCTAATATGTGCCCTA+AGG + chr4.4:26894760-26894779 MS.gene059531:intron 40.0%
TGTGACTGCTGGATTGAAAT+GGG - chr4.4:26894263-26894282 None:intergenic 40.0%
TGTTGTATCTGCAGTTTCCA+CGG + chr4.4:26894660-26894679 MS.gene059531:CDS 40.0%
!! CCCTTTGTTGGTGCTATATT+AGG + chr4.4:26895232-26895251 MS.gene059531:CDS 40.0%
!! CGTAGTGGTTCTGAATAAAG+TGG + chr4.4:26894141-26894160 MS.gene059531:CDS 40.0%
!! TTTTGAGGAAGCTTGAGCTT+CGG - chr4.4:26895312-26895331 None:intergenic 40.0%
!!! GTGTCTCTAGAGCAAGTTTT+CGG + chr4.4:26895332-26895351 MS.gene059531:CDS 40.0%
!!! TTGCTCTAGAGACACTTTTG+AGG - chr4.4:26895327-26895346 None:intergenic 40.0%
!!! TTTTTTGGAACAGTACCAGC+AGG + chr4.4:26894583-26894602 MS.gene059531:CDS 40.0%
AGTTTCCACGGACAATAGAG+CGG + chr4.4:26894672-26894691 MS.gene059531:CDS 45.0%
CAGGTAATAGCAGAGGTGAT+AGG + chr4.4:26894088-26894107 MS.gene059531:intron 45.0%
CCCCATGTTACACAAATTCC+AGG - chr4.4:26894193-26894212 None:intergenic 45.0%
CTGTGACTGCTGGATTGAAA+TGG - chr4.4:26894264-26894283 None:intergenic 45.0%
CTTCGGCTTAATTCTCTGAG+TGG - chr4.4:26895295-26895314 None:intergenic 45.0%
GCCATTATATCTTGCTGCAC+AGG + chr4.4:26894498-26894517 MS.gene059531:CDS 45.0%
GTAGTAGACGTCTTCATTGC+TGG + chr4.4:26895005-26895024 MS.gene059531:CDS 45.0%
TAGATGTGCATCACTACAGC+AGG - chr4.4:26895184-26895203 None:intergenic 45.0%
TCATTGATGCACCTGACACA+GGG - chr4.4:26895141-26895160 None:intergenic 45.0%
TCGTCATTTCCCGCTTAAAC+AGG + chr4.4:26894300-26894319 MS.gene059531:CDS 45.0%
TTCATTGATGCACCTGACAC+AGG - chr4.4:26895142-26895161 None:intergenic 45.0%
! AATCCCTAACTTGTGCCCTT+AGG - chr4.4:26894779-26894798 None:intergenic 45.0%
! ATCCCTAACTTGTGCCCTTA+GGG - chr4.4:26894778-26894797 None:intergenic 45.0%
! ATGAACCCAGCCAGAACTTT+TGG + chr4.4:26895157-26895176 MS.gene059531:CDS 45.0%
! TGAACCCAGCCAGAACTTTT+GGG + chr4.4:26895158-26895177 MS.gene059531:CDS 45.0%
ACTAACCGCTCTATTGTCCG+TGG - chr4.4:26894680-26894699 None:intergenic 50.0%
CTAGGATCTGTCCTTGCTAG+TGG + chr4.4:26894523-26894542 MS.gene059531:CDS 50.0%
GAATTGGCAGGGATTGCTGT+TGG + chr4.4:26894972-26894991 MS.gene059531:CDS 50.0%
GGCACAATGTTCCACTAGCA+AGG - chr4.4:26894537-26894556 None:intergenic 50.0%
TACAGCAGGCCCAAAAGTTC+TGG - chr4.4:26895170-26895189 None:intergenic 50.0%
TATCTTGCTGCACAGGTGCT+AGG + chr4.4:26894505-26894524 MS.gene059531:CDS 50.0%
! TGCCCTAAGGGCACAAGTTA+GGG + chr4.4:26894773-26894792 MS.gene059531:intron 50.0%
!! AGGGTTTTGTGGTTCTCCTG+AGG + chr4.4:26893900-26893919 MS.gene059531:CDS 50.0%
!! ATTTGCAGGGTGTTGCGTAG+TGG + chr4.4:26894126-26894145 MS.gene059531:CDS 50.0%
ATTGCATGCAGCCCTGTGTC+AGG + chr4.4:26895127-26895146 MS.gene059531:intron 55.0%
GCGAAGCTAACTGTGACTGC+TGG - chr4.4:26894274-26894293 None:intergenic 55.0%
! CAGGCCCAAAAGTTCTGGCT+GGG - chr4.4:26895165-26895184 None:intergenic 55.0%
! GTGCCCTAAGGGCACAAGTT+AGG + chr4.4:26894772-26894791 MS.gene059531:intron 55.0%
! GCAGGCCCAAAAGTTCTGGC+TGG - chr4.4:26895166-26895185 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 26893859 26895357 26893859 ID=MS.gene059531
chr4.4 mRNA 26893859 26895357 26893859 ID=MS.gene059531.t1;Parent=MS.gene059531
chr4.4 exon 26893859 26893942 26893859 ID=MS.gene059531.t1.exon1;Parent=MS.gene059531.t1
chr4.4 CDS 26893859 26893942 26893859 ID=cds.MS.gene059531.t1;Parent=MS.gene059531.t1
chr4.4 exon 26894091 26894321 26894091 ID=MS.gene059531.t1.exon2;Parent=MS.gene059531.t1
chr4.4 CDS 26894091 26894321 26894091 ID=cds.MS.gene059531.t1;Parent=MS.gene059531.t1
chr4.4 exon 26894496 26894693 26894496 ID=MS.gene059531.t1.exon3;Parent=MS.gene059531.t1
chr4.4 CDS 26894496 26894693 26894496 ID=cds.MS.gene059531.t1;Parent=MS.gene059531.t1
chr4.4 exon 26894966 26895027 26894966 ID=MS.gene059531.t1.exon4;Parent=MS.gene059531.t1
chr4.4 CDS 26894966 26895027 26894966 ID=cds.MS.gene059531.t1;Parent=MS.gene059531.t1
chr4.4 exon 26895138 26895357 26895138 ID=MS.gene059531.t1.exon5;Parent=MS.gene059531.t1
chr4.4 CDS 26895138 26895357 26895138 ID=cds.MS.gene059531.t1;Parent=MS.gene059531.t1
Gene Sequence

>MS.gene059531

ATGGAAGAAGGTGATCTTAAGGCACAAACAAAAAGTGAATCAGGGTTTTGTGGTTCTCCTGAGGTAGTTCAAATCATACAAAAGGTACACATATTAATAATTCTACCATTGTACCATACAATCACATTAATAATATATAGTTTTCATTTATATAGTTACCATTGTACCATTGTACACATATCTTTGTCACTACTAATGAATGTTTTATTTAAAAATGAAATGTATGCAACAGGTAATAGCAGAGGTGATAGGAACATACTTCTTAATATTTGCAGGGTGTTGCGTAGTGGTTCTGAATAAAGTGGAAGAAACTAAAGGAACAGTAACATTTCCTGGAATTTGTGTAACATGGGGTCTATCAGTTATGATCTTGGTTTATTCTCTTGGTCACATCTCTGGTGCCCATTTCAATCCAGCAGTCACAGTTAGCTTCGCAATCTATCGTCATTTCCCGCTTAAACAGGCATGCATATACTTATGACAACTTGATCTACTCAGCTCACATTGTTATAAAAAGTTGAATTAACGAAAAAAATAATTACTCTCCCATTTTGTCGTCTCAAACACTTAACGACTGAATTAGAGATGGATTTCTTGTTTTTCGAACATATGTAACATAAATTGTTGATTTTTGCAGGTGCCATTATATCTTGCTGCACAGGTGCTAGGATCTGTCCTTGCTAGTGGAACATTGTGCCTTCTTTTTGATGTAGATGAAAAATCTTTTTTTGGAACAGTACCAGCAGGTTCTAATATTCAATCTCTTGTCTTTGAGATACTCACATCATTTCTCTTGATGTTTGTTGTATCTGCAGTTTCCACGGACAATAGAGCGGTTAGTATACTCAGTTTAGTTTAATTTACCTTATATTTTCTTGCATTTTGGTATATAAAATTAGGGTCATGCTAATATGTGCCCTAAGGGCACAAGTTAGGGATTCCACTAATAGTAAGTATTACTGAGAAACATTAATTTTCAATTTTTTAATAATAAATTGCCACTATTTTCAAAACAAAATTTCTATACTAGGTTCATTCACATGTGCCCTAAGACACATGTTAGCCTTTTCCATAAAATTAATTCAAATTCCTCTTTTTATCAATCAGATTGGAGAATTGGCAGGGATTGCTGTTGGCATGACAATCGTAGTAGACGTCTTCATTGCTGGGTAATTTCAACACTATTACTATATATATAGTTTCAATGGTTTTACTTATCATAATGAAGTCCATATATATACTGCTGCAATTTAATCCTAAGTTTGATAATTGCATGCAGCCCTGTGTCAGGTGCATCAATGAACCCAGCCAGAACTTTTGGGCCTGCTGTAGTGATGCACATCTACAAAGGATTTTGGGTTTATATAGTTGGACCCTTTGTTGGTGCTATATTAGGTGCATCAGCTTACAACTTGATTAGATTTACTGATAAACCACTCAGAGAATTAAGCCGAAGCTCAAGCTTCCTCAAAAGTGTCTCTAGAGCAAGTTTTCGGTAG
Protein sequence

>MS.gene059531.t1

MEEGDLKAQTKSESGFCGSPEVVQIIQKVIAEVIGTYFLIFAGCCVVVLNKVEETKGTVTFPGICVTWGLSVMILVYSLGHISGAHFNPAVTVSFAIYRHFPLKQVPLYLAAQVLGSVLASGTLCLLFDVDEKSFFGTVPAGSNIQSLVFEILTSFLLMFVVSAVSTDNRAIGELAGIAVGMTIVVDVFIAGPVSGASMNPARTFGPAVVMHIYKGFWVYIVGPFVGAILGASAYNLIRFTDKPLRELSRSSSFLKSVSRASFR