AlfalfaGEDB | MS.gene022283

M. sativa cultivar XinJiangDaYe / MS.gene022283

KEGG: K10881
GO: GO:0005575 GO:0006405 GO:0006406 GO:0006810 GO:0006913 GO:0008150 GO:0008541 GO:0009987 GO:0015931 GO:0016043 GO:0022607 GO:0032991 GO:0034622 GO:0043248 GO:0043933 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0065003 GO:0071702 GO:0071705 GO:0071840
PFAM: PF05160.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022283.t1	XP_013461011.1	100	73	0	0	1	73	1	73	9.20E-08	65.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022283.t1	I3SYL1	100.0	73	0	0	1	73	1	73	6.6e-08	65.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene051630	MS.gene022283	PPI
MS.gene67460	MS.gene022283	PPI
MS.gene01697	MS.gene022283	PPI
MS.gene24375	MS.gene022283	PPI
MS.gene01284	MS.gene022283	PPI
MS.gene36633	MS.gene022283	PPI
MS.gene67099	MS.gene022283	PPI
MS.gene057145	MS.gene022283	PPI
MS.gene21169	MS.gene022283	PPI
MS.gene28454	MS.gene022283	PPI
MS.gene012127	MS.gene022283	PPI
MS.gene24542	MS.gene022283	PPI
MS.gene09974	MS.gene022283	PPI
MS.gene004328	MS.gene022283	PPI
MS.gene70283	MS.gene022283	PPI
MS.gene48400	MS.gene022283	PPI
MS.gene70282	MS.gene022283	PPI
MS.gene030685	MS.gene022283	PPI
MS.gene064786	MS.gene022283	PPI
MS.gene64789	MS.gene022283	PPI
MS.gene42312	MS.gene022283	PPI
MS.gene57218	MS.gene022283	PPI
MS.gene52815	MS.gene022283	PPI
MS.gene072221	MS.gene022283	PPI
MS.gene69373	MS.gene022283	PPI
MS.gene002648	MS.gene022283	PPI
MS.gene024828	MS.gene022283	PPI
MS.gene69461	MS.gene022283	PPI
MS.gene064694	MS.gene022283	PPI
MS.gene017622	MS.gene022283	PPI
MS.gene013607	MS.gene022283	PPI
MS.gene89672	MS.gene022283	PPI
MS.gene002306	MS.gene022283	PPI
MS.gene68079	MS.gene022283	PPI
MS.gene02265	MS.gene022283	PPI
MS.gene48279	MS.gene022283	PPI
MS.gene48281	MS.gene022283	PPI
MS.gene014485	MS.gene022283	PPI
MS.gene06729	MS.gene022283	PPI
MS.gene58136	MS.gene022283	PPI
MS.gene062770	MS.gene022283	PPI
MS.gene58134	MS.gene022283	PPI
MS.gene033335	MS.gene022283	PPI
MS.gene025808	MS.gene022283	PPI
MS.gene072220	MS.gene022283	PPI
MS.gene40486	MS.gene022283	PPI
MS.gene21801	MS.gene022283	PPI
MS.gene06946	MS.gene022283	PPI
MS.gene28183	MS.gene022283	PPI
MS.gene030686	MS.gene022283	PPI
MS.gene59485	MS.gene022283	PPI
MS.gene011376	MS.gene022283	PPI
MS.gene014393	MS.gene022283	PPI
MS.gene057144	MS.gene022283	PPI
MS.gene36746	MS.gene022283	PPI
MS.gene32974	MS.gene022283	PPI
MS.gene044013	MS.gene022283	PPI
MS.gene44230	MS.gene022283	PPI
MS.gene011065	MS.gene022283	PPI
MS.gene058061	MS.gene022283	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022283.t1	MTR_3g078130	100.000	73	0	0	1	73	1	73	4.87e-44	136

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022283.t1	AT1G64750	76.712	73	16	1	1	72	1	73	3.24e-11	53.5
MS.gene022283.t1	AT1G64750	76.712	73	16	1	1	72	1	73	3.24e-11	53.5

Find 17 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAACAATGGGAGGATGAT+TGG	0.329365	3.3:-66286500	MS.gene022283:CDS
TCAACAATGGGAGGATGATT+GGG	0.443446	3.3:-66286499	MS.gene022283:CDS
ATTTCTCTGTTCAGCTGAGA+AGG	0.461195	3.3:-66286455	MS.gene022283:CDS
TGTTCAGCTGAGAAGGGAGT+TGG	0.494913	3.3:-66286448	MS.gene022283:CDS
GAAAGAAGTAACTCAACAAT+GGG	0.498445	3.3:-66286511	MS.gene022283:CDS
GAAGAGTTTGAAATCAATGA+AGG	0.502774	3.3:-66287589	MS.gene022283:intron
GAGTGGGATGACATGGAGGA+AGG	0.532354	3.3:-66286534	MS.gene022283:CDS
TTTCTCTGTTCAGCTGAGAA+GGG	0.558420	3.3:-66286454	MS.gene022283:CDS
AGCCTTTGCTTCAGTCGCCA+TGG	0.560336	3.3:+66287660	None:intergenic
GGAAAGAAGTAACTCAACAA+TGG	0.565472	3.3:-66286512	MS.gene022283:CDS
ATCCATGGCGACTGAAGCAA+AGG	0.576258	3.3:-66287662	None:intergenic
ACTTGCAGAGTGGGATGACA+TGG	0.578383	3.3:-66286541	MS.gene022283:intron
AGTGGGATGACATGGAGGAA+GGG	0.591985	3.3:-66286533	MS.gene022283:CDS
TGAAGCAAAGGCTGTGACCG+AGG	0.600680	3.3:-66287650	MS.gene022283:CDS
TAGATCGATCTTGACATCCT+CGG	0.618819	3.3:+66287633	None:intergenic
AGAAGTAACTCAACAATGGG+AGG	0.631574	3.3:-66286508	MS.gene022283:CDS
TGCAGAGTGGGATGACATGG+AGG	0.657206	3.3:-66286538	MS.gene022283:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTTTCAAATTACATGATT+GGG	+	chr3.3:66287028-66287047	None:intergenic	20.0%
!!	TAAGTTTCAAATTACATGAT+TGG	+	chr3.3:66287029-66287048	None:intergenic	20.0%
!	AACATCACTGAAAATTTCTT+AGG	+	chr3.3:66286598-66286617	None:intergenic	25.0%
!	ATTGGCATTTGTATGTTAAT+TGG	-	chr3.3:66287402-66287421	MS.gene022283:intron	25.0%
!	ATTTCATACACGAAATAAAG+TGG	+	chr3.3:66287069-66287088	None:intergenic	25.0%
!	CTTATACATAAGCACTTATT+TGG	-	chr3.3:66286949-66286968	MS.gene022283:intron	25.0%
!!	AGTTGTTTCATAAACTATCA+TGG	+	chr3.3:66286875-66286894	None:intergenic	25.0%
!!	TGTTAATCCTATTTCTCATT+AGG	-	chr3.3:66287316-66287335	MS.gene022283:intron	25.0%
!!!	TGAATCTCATTTAGTTTGAT+TGG	-	chr3.3:66287384-66287403	MS.gene022283:intron	25.0%
	ACAGCACTTATATACTAGAT+AGG	+	chr3.3:66286767-66286786	None:intergenic	30.0%
	CAAATTACATGATTGGGTTA+TGG	+	chr3.3:66287022-66287041	None:intergenic	30.0%
	GAAAGAAGTAACTCAACAAT+GGG	-	chr3.3:66287572-66287591	MS.gene022283:intron	30.0%
	TTGGCATTCAATTAATTACG+AGG	-	chr3.3:66286724-66286743	MS.gene022283:intron	30.0%
	TTTCTTGACGACTTGAAATT+AGG	-	chr3.3:66287444-66287463	MS.gene022283:intron	30.0%
!	GAACATTTGTTAGACTGTTT+GGG	-	chr3.3:66286804-66286823	MS.gene022283:intron	30.0%
!	GAAGAGTTTGAAATCAATGA+AGG	-	chr3.3:66286494-66286513	MS.gene022283:CDS	30.0%
!	TCTAGTATATAAGTGCTGTT+TGG	-	chr3.3:66286768-66286787	MS.gene022283:intron	30.0%
!	TGAACATTTGTTAGACTGTT+TGG	-	chr3.3:66286803-66286822	MS.gene022283:intron	30.0%
	ACATTAGATTGTGAGTGCAA+TGG	+	chr3.3:66287486-66287505	None:intergenic	35.0%
	ATACACGAAATAAAGTGGTG+AGG	+	chr3.3:66287064-66287083	None:intergenic	35.0%
	ATTAGGCATGAACACTTCTA+GGG	-	chr3.3:66287333-66287352	MS.gene022283:intron	35.0%
	GCATTCAATTAATTACGAGG+AGG	-	chr3.3:66286727-66286746	MS.gene022283:intron	35.0%
	GCTGCATCATTTATATAAGC+AGG	+	chr3.3:66287171-66287190	None:intergenic	35.0%
	GGAAAGAAGTAACTCAACAA+TGG	-	chr3.3:66287571-66287590	MS.gene022283:intron	35.0%
	GTTCATGCCTAATGAGAAAT+AGG	+	chr3.3:66287326-66287345	None:intergenic	35.0%
	TTACATGATTGGGTTATGGT+TGG	+	chr3.3:66287018-66287037	None:intergenic	35.0%
	TTCAAGTCGTCAAGAAAAGT+TGG	+	chr3.3:66287441-66287460	None:intergenic	35.0%
!	GACTTGAAATTAGGAGGTTT+TGG	-	chr3.3:66287453-66287472	MS.gene022283:intron	35.0%
!	TGTTTTACAACTTGCAGAGT+GGG	-	chr3.3:66287533-66287552	MS.gene022283:intron	35.0%
!!	ACTTCTAGGGCGAAAATTAT+AGG	-	chr3.3:66287346-66287365	MS.gene022283:intron	35.0%
!!	TATTCATTAGTTTGCTTCCG+TGG	-	chr3.3:66286641-66286660	MS.gene022283:intron	35.0%
!!	TTGTTTTACAACTTGCAGAG+TGG	-	chr3.3:66287532-66287551	MS.gene022283:intron	35.0%
	AGAAGTAACTCAACAATGGG+AGG	-	chr3.3:66287575-66287594	MS.gene022283:intron	40.0%
	AGACTGTTTGGGAGAACTTA+TGG	-	chr3.3:66286815-66286834	MS.gene022283:intron	40.0%
	ATTTCTCTGTTCAGCTGAGA+AGG	-	chr3.3:66287628-66287647	MS.gene022283:CDS	40.0%
	CATTAGGCATGAACACTTCT+AGG	-	chr3.3:66287332-66287351	MS.gene022283:intron	40.0%
	CTTGACGACTTGAAATTAGG+AGG	-	chr3.3:66287447-66287466	MS.gene022283:intron	40.0%
	GTCGTCAAGAAAAGTTGGAT+AGG	+	chr3.3:66287436-66287455	None:intergenic	40.0%
	TAGATCGATCTTGACATCCT+CGG	+	chr3.3:66286453-66286472	None:intergenic	40.0%
	TCAACAATGGGAGGATGATT+GGG	-	chr3.3:66287584-66287603	MS.gene022283:intron	40.0%
	TCCCTAATTCGTTTCTTCCA+CGG	+	chr3.3:66286661-66286680	None:intergenic	40.0%
	TCCGTGGAAGAAACGAATTA+GGG	-	chr3.3:66286657-66286676	MS.gene022283:intron	40.0%
	TGAGCTAGACTTTGTGTGTT+TGG	-	chr3.3:66286705-66286724	MS.gene022283:intron	40.0%
	TTCCGTGGAAGAAACGAATT+AGG	-	chr3.3:66286656-66286675	MS.gene022283:intron	40.0%
	TTTCTCTGTTCAGCTGAGAA+GGG	-	chr3.3:66287629-66287648	MS.gene022283:CDS	40.0%
	ATGCCACTACTCTCTTCTCA+TGG	-	chr3.3:66287292-66287311	MS.gene022283:intron	45.0%
	CTCAACAATGGGAGGATGAT+TGG	-	chr3.3:66287583-66287602	MS.gene022283:intron	45.0%
	TGCCACTACTCTCTTCTCAT+GGG	-	chr3.3:66287293-66287312	MS.gene022283:intron	45.0%
	ACTTGCAGAGTGGGATGACA+TGG	-	chr3.3:66287542-66287561	MS.gene022283:intron	50.0%
	AGTGGGATGACATGGAGGAA+GGG	-	chr3.3:66287550-66287569	MS.gene022283:intron	50.0%
	CACCCATGAGAAGAGAGTAG+TGG	+	chr3.3:66287298-66287317	None:intergenic	50.0%
	TGTTCAGCTGAGAAGGGAGT+TGG	-	chr3.3:66287635-66287654	MS.gene022283:CDS	50.0%
	GAGTGGGATGACATGGAGGA+AGG	-	chr3.3:66287549-66287568	MS.gene022283:intron	55.0%
	TGAAGCAAAGGCTGTGACCG+AGG	-	chr3.3:66286433-66286452	MS.gene022283:CDS	55.0%
	TGCAGAGTGGGATGACATGG+AGG	-	chr3.3:66287545-66287564	MS.gene022283:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	66286425	66287680	66286425	ID=MS.gene022283
chr3.3	mRNA	66286425	66287680	66286425	ID=MS.gene022283.t1;Parent=MS.gene022283
chr3.3	exon	66287590	66287680	66287590	ID=MS.gene022283.t1.exon1;Parent=MS.gene022283.t1
chr3.3	CDS	66287590	66287680	66287590	ID=cds.MS.gene022283.t1;Parent=MS.gene022283.t1
chr3.3	exon	66286425	66286555	66286425	ID=MS.gene022283.t1.exon2;Parent=MS.gene022283.t1
chr3.3	CDS	66286425	66286555	66286425	ID=cds.MS.gene022283.t1;Parent=MS.gene022283.t1

Gene Sequence

>MS.gene022283

ATGGCGACTGAAGCAAAGGCTGTGACCGAGGATGTCAAGATCGATCTATTCGAAGACGATGATGAGTTCGAAGAGTTTGAAATCAATGAAGGTATGAATCAGTAAACAACGTTGCTTCGCTGATGCAATTATCTTTGCTTCTGTTTTAAGTTTACATTTAGTTAAGTGCTCCTAAGAAATTTTCAGTGATGTTATCATCAAATGATTTATAAGTTATATTCATTAGTTTGCTTCCGTGGAAGAAACGAATTAGGGATTTCATCGTGTATTGTTGCACATTTGAGCTAGACTTTGTGTGTTTGGCATTCAATTAATTACGAGGAGGTTATGAAAATTTAACCTATCTAGTATATAAGTGCTGTTTGGATTTGAGTAACATGAACATTTGTTAGACTGTTTGGGAGAACTTATGGAAACAACTTATGACAGATCACTTTCATAAGCTCTCCATGATAGTTTATGAAACAACTTATAACTTATATGAAAATAGTTTGAATTTATTTTATGCTTTGTTATAAAAACTGCTTATACATAAGCACTTATTTGGTAAGCGCTTGTGCTATAAGCACTTAATTAATTAAGCTGTATGTCCAACCATAACCCAATCATGTAATTTGAAACTTATTTGATACTTCACCTCACCACTTTATTTCGTGTATGAAATAATATGATATTTAAATATACATATCTACTATTAAATCATATATACTTGCAAATGTCTAAACTTTTATATACATTATTGTCCTGCTTATATAAATGATGCAGCATTTAGATTTGCTAGATTCACGTTGTACACAGCGTTAATGAAGATGTAAGTTTGCTAATTTTTTGTGCGTGTGTCTCACAGTGCATGCAAACTGTAATTTTATGCCACTACTCTCTTCTCATGGGTGTTAATCCTATTTCTCATTAGGCATGAACACTTCTAGGGCGAAAATTATAGGACTTATTTAATTGCTTGAATCTCATTTAGTTTGATTGGCATTTGTATGTTAATTGGCTTCACTGCCTATCCAACTTTTCTTGACGACTTGAAATTAGGAGGTTTTGGATTTCTGCCATTGCACTCACAATCTAATGTTGCTAACTATTTCTTTTTGCGCTTCTTTGTTTTACAACTTGCAGAGTGGGATGACATGGAGGAAGGGAAAGAAGTAACTCAACAATGGGAGGATGATTGGGATGATGATGATGTTAGCGATGATTTCTCTGTTCAGCTGAGAAGGGAGTTGGAGAAGACTACTGATAAGAATTAA

Protein sequence

>MS.gene022283.t1

MATEAKAVTEDVKIDLFEDDDEFEEFEINEEWDDMEEGKEVTQQWEDDWDDDDVSDDFSVQLRRELEKTTDKN